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Nurhan Arslan - One of the best experts on this subject based on the ideXlab platform.
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flow properties of Sugar Beet Pulp cellulose and intrinsic viscosity molecular weight relationship
Carbohydrate Polymers, 2003Co-Authors: Hasan Togrul, Nurhan ArslanAbstract:Abstract Cellulose was extracted with 5, 10, 15% NaOH at 25, 35, 45 °C for 10, 16, 22 h from defatted, protein, pectin and hemicellulose free, delignified Sugar Beet Pulp. Extraction with 10% NaOH at 35 °C for 22 h gave the highest yield of the removal of substances with the exception of cellulose. The effects of temperature and concentration on the viscosity of Sugar Beet Pulp cellulose solutions were examined at different temperatures and concentration levels. Twenty-eight different model equations that describe the combined effects of temperature and concentration on the viscosity were derived. Theoretical models describing the temperature and concentration dependence of viscosity were fitted to the experimental data and the model parameters in equations were determined by multiple regression analysis of the experimental data in the temperature range 10–60 °C and in the concentration range 0.5–10 kg/m 3 . The viscosity of cellulose from Sugar Beet Pulp can be predicted by the single equation: ln η=−2.8C 0.0186 +1031C 0.025 /T+2654 exp (0.1896C)/T 2 . The average molecular weight was measured by light scattering technique. Intrinsic viscosities and molecular weights of cellulose obtained at different extraction conditions ranged between 0.347–0.0836 m 3 /kg and 303,200–893,500 kg/kg mol, respectively. The molecular weight dependence of the intrinsic viscosity of the Sugar Beet Pulp cellulose solutions was expressed by Mark-Houwink-Sakurada equation. The intrinsic viscosity–molecular weight relationship was found as η i =2.313×10 −6 ( M w,ave ) 0.7665 .
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Flow properties of Sugar Beet Pulp cellulose and intrinsic viscosity–molecular weight relationship
Carbohydrate Polymers, 2003Co-Authors: Hasan Togrul, Nurhan ArslanAbstract:Abstract Cellulose was extracted with 5, 10, 15% NaOH at 25, 35, 45 °C for 10, 16, 22 h from defatted, protein, pectin and hemicellulose free, delignified Sugar Beet Pulp. Extraction with 10% NaOH at 35 °C for 22 h gave the highest yield of the removal of substances with the exception of cellulose. The effects of temperature and concentration on the viscosity of Sugar Beet Pulp cellulose solutions were examined at different temperatures and concentration levels. Twenty-eight different model equations that describe the combined effects of temperature and concentration on the viscosity were derived. Theoretical models describing the temperature and concentration dependence of viscosity were fitted to the experimental data and the model parameters in equations were determined by multiple regression analysis of the experimental data in the temperature range 10–60 °C and in the concentration range 0.5–10 kg/m3. The viscosity of cellulose from Sugar Beet Pulp can be predicted by the single equation: ln η=−2.8C 0.0186 +1031C 0.025 /T+2654 exp (0.1896C)/T 2 . The average molecular weight was measured by light scattering technique. Intrinsic viscosities and molecular weights of cellulose obtained at different extraction conditions ranged between 0.347–0.0836 m3/kg and 303,200–893,500 kg/kg mol, respectively. The molecular weight dependence of the intrinsic viscosity of the Sugar Beet Pulp cellulose solutions was expressed by Mark-Houwink-Sakurada equation. The intrinsic viscosity–molecular weight relationship was found as ηi=2.313×10−6(Mw,ave)0.7665.
Hasan Togrul - One of the best experts on this subject based on the ideXlab platform.
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flow properties of Sugar Beet Pulp cellulose and intrinsic viscosity molecular weight relationship
Carbohydrate Polymers, 2003Co-Authors: Hasan Togrul, Nurhan ArslanAbstract:Abstract Cellulose was extracted with 5, 10, 15% NaOH at 25, 35, 45 °C for 10, 16, 22 h from defatted, protein, pectin and hemicellulose free, delignified Sugar Beet Pulp. Extraction with 10% NaOH at 35 °C for 22 h gave the highest yield of the removal of substances with the exception of cellulose. The effects of temperature and concentration on the viscosity of Sugar Beet Pulp cellulose solutions were examined at different temperatures and concentration levels. Twenty-eight different model equations that describe the combined effects of temperature and concentration on the viscosity were derived. Theoretical models describing the temperature and concentration dependence of viscosity were fitted to the experimental data and the model parameters in equations were determined by multiple regression analysis of the experimental data in the temperature range 10–60 °C and in the concentration range 0.5–10 kg/m 3 . The viscosity of cellulose from Sugar Beet Pulp can be predicted by the single equation: ln η=−2.8C 0.0186 +1031C 0.025 /T+2654 exp (0.1896C)/T 2 . The average molecular weight was measured by light scattering technique. Intrinsic viscosities and molecular weights of cellulose obtained at different extraction conditions ranged between 0.347–0.0836 m 3 /kg and 303,200–893,500 kg/kg mol, respectively. The molecular weight dependence of the intrinsic viscosity of the Sugar Beet Pulp cellulose solutions was expressed by Mark-Houwink-Sakurada equation. The intrinsic viscosity–molecular weight relationship was found as η i =2.313×10 −6 ( M w,ave ) 0.7665 .
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Flow properties of Sugar Beet Pulp cellulose and intrinsic viscosity–molecular weight relationship
Carbohydrate Polymers, 2003Co-Authors: Hasan Togrul, Nurhan ArslanAbstract:Abstract Cellulose was extracted with 5, 10, 15% NaOH at 25, 35, 45 °C for 10, 16, 22 h from defatted, protein, pectin and hemicellulose free, delignified Sugar Beet Pulp. Extraction with 10% NaOH at 35 °C for 22 h gave the highest yield of the removal of substances with the exception of cellulose. The effects of temperature and concentration on the viscosity of Sugar Beet Pulp cellulose solutions were examined at different temperatures and concentration levels. Twenty-eight different model equations that describe the combined effects of temperature and concentration on the viscosity were derived. Theoretical models describing the temperature and concentration dependence of viscosity were fitted to the experimental data and the model parameters in equations were determined by multiple regression analysis of the experimental data in the temperature range 10–60 °C and in the concentration range 0.5–10 kg/m3. The viscosity of cellulose from Sugar Beet Pulp can be predicted by the single equation: ln η=−2.8C 0.0186 +1031C 0.025 /T+2654 exp (0.1896C)/T 2 . The average molecular weight was measured by light scattering technique. Intrinsic viscosities and molecular weights of cellulose obtained at different extraction conditions ranged between 0.347–0.0836 m3/kg and 303,200–893,500 kg/kg mol, respectively. The molecular weight dependence of the intrinsic viscosity of the Sugar Beet Pulp cellulose solutions was expressed by Mark-Houwink-Sakurada equation. The intrinsic viscosity–molecular weight relationship was found as ηi=2.313×10−6(Mw,ave)0.7665.
J.-f. Thibault - One of the best experts on this subject based on the ideXlab platform.
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binding of divalent metal cations by Sugar Beet Pulp
Carbohydrate Polymers, 1997Co-Authors: V M Dronnet, Monique A V Axelos, Catherine M.g.c. Renard, J.-f. ThibaultAbstract:The binding of divalent metal cations (Ca2+, Cd2+, Cu2+, Ni2+, Pb2+ and Zn2+) by Sugar-Beet Pulp was studied. In the presence of 0.1 m NaNO3 the level of metal cation uptake was found to reach its maximum value very rapidly with the speed increasing both with the Sugar-Beet Pulp concentration and with increasing initial pH of the suspension. Using a pH-metric method and by comparing binding isotherms drawn by measuring the free cation concentration after equilibration, a clear scale of decreasing selectivity was found as follows: Cu2+~Pb2+⪢Cd2+~Zn2+ > Ni2+ > Ca2+. Binding followed the Langmuir-type isotherm except for Ca2+. Adsorption may contribute to the binding phenomenon in addition to ion exchange, which may include electrostatic interactions and even chelation in the case of the more strongly bound cations. Sugar-Beet Pulp, which is cheap and highly selective, therefore seems to be a promising substrate to entrap heavy metals in aqueous solutions.
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enzymatic saccharification of Sugar Beet Pulp
Enzyme and Microbial Technology, 1996Co-Authors: Valérie Micard, Catherine M.g.c. Renard, J.-f. ThibaultAbstract:Abstract Nine commercial enzymatic preparations have been tested for their ability to release ferulic acid, rhamnose, arabinose, and galacturonic acid from Sugar-Beet Pulp. SP 584, SP 585, and SP 342 from Novo Nordisk gave the highest release of ferulic acid, arabinose, and rhamnose. SP 584 and SP 585 degraded the Pulp more rapidly than SP 342. Only SP 342 released free ferulic acid mainly in the early stages of hydrolysis; the ferulic acid was later released under esterified form. Admixtures of SP 342 or SP 249 with SP 584 or SP 585 released no free ferulic acid. The optimal pH for degradation of Sugar-Beet Pulp by SP 584 in acetate buffer was 4; however, more (or similar levels of) galacturonic acid, arabinose, rhamnose, and galactose were solubilized when the reaction was carried out in water with faster release of galactose, arabinose, and galacturonic acid. A larger-scale hydrolysis by SP 584, carried out in water with a longer incubation time (120 h) and a higher enzyme/Sugar-Beet Pulp ratio (3%), resulted in release of ∼70% of ferulic acid and 65% of diferulic acid, ∼80% of galacturonic acid (92% as monomers) and ∼70% of the Pulp neutral Sugars (∼70–85% as monomers).
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structure identification of feruloylated oligosaccharides from Sugar Beet Pulp by nmr spectroscopy
Carbohydrate Research, 1994Co-Authors: Ian J Colquhoun, J.-f. Thibault, Mariechristine Ralet, Craig B Faulds, Gary WilliamsonAbstract:1D NMR (1H and 13C) and 2D NMR spectroscopy have been used to determine the structure of feruloylated oligosaccharides obtained by enzymic degradation or mild acid hydrolysis of Sugar-Beet Pulp. Feruloylated oligosaccharides derived from pectic neutral side-chains containing arabinose or galactose residues were identified. In the feruloylated arabinose oligosaccharides, feruloyl groups were linked to 0-2 of l,-Araf residues. The structure of the feruloylated arabinose disaccharide was identified as 0-[2-0-(trans-feruloyl)-α-l-Ara f]-(1 → 5)-l-Araf and that of the feruloylated arabinose trisaccharide as O-α-l-Araf-(1 → 3)-O-[2-0-(trans-feruloyl)-α-l-Araf]-(1 → 5)-l-Araf. The structure of the feruloylated galactose disaccharide was identified as 0-[6-0-(trans-feruloyl)-β-d-Galp]-(1 → 4)-d-Galp. From our results, we suggest that the feruloyl groups present in Sugar-Beet Pulp are linked to the arabinofuranosyl residues of the main core of α-(1 → 5)-linked arabinan chains and to the galactopyranosyl residues of the main core of β-(1 → 4)-linked type I galactan chains.
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studies on enzymic release of ferulic acid from Sugar Beet Pulp
Lwt - Food Science and Technology, 1994Co-Authors: Valérie Micard, Catherine M.g.c. Renard, J.-f. ThibaultAbstract:Abstract Eighteen commercial enzymic preparations have been tested for their ability to release ferulic acid from primary cell-walls of Sugar-Beet Pulp. Three release patterns were found: (a) no or little, esterified ferulic acid; (b) only esterified ferulic acid; or (c) a mixture of free and esterified ferulic acid. No enzymic complex released only free ferulic acid. Release kinetics were studied on two complexes, 'Hemicellulase REG II' (Gist-Brocades), which released the highest amount of esterified ferulic acid, and 'Pektolase LM' (Grindsted), which released one of the highest amounts of free ferulic acid with the lowest proportion of esterified ferulic acid. 'Pektolase LM' digested Sugar-Beet Pulp rapidly, first releasing esterified ferulic acid, which was later deesterified. 'Hemicellulase REG II' showed an initial lag phase followed by fast liberation (between 32 and 48 h of incubation) of esterified ferulic acid. Sugar-Beet Pulp hydrolysates from 'Pektolase LM' and 'Hemicellulase REG II' were fractionated on DEAE Sephacel. The results showed that esterified ferulic acid was present either linked to fragments of pectic polysaccharides poor in arabinose (∼40% of esterified ferulic acid injected) or to arabinose oligomers (∼60%).
Joanna Berlowska - One of the best experts on this subject based on the ideXlab platform.
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Enzymatic Conversion of Sugar Beet Pulp: A Comparison of Simultaneous Saccharification and Fermentation and Separate Hydrolysis and Fermentation for Lactic Acid Production.
Food Technology and Biotechnology, 2018Co-Authors: Joanna Berlowska, Dorota Kregiel, Sebastian Borowski, Weronika Cieciura-włoch, Halina Kalinowska, Ewelina Pawlikowska, Michał Binczarski, Izabela WitońskaAbstract:: This study compares the efficiency of lactic acid production by separate hydrolysis and fermentation (SHF) or simultaneous saccharification and fermentation (SSF) of Sugar Beet Pulp, a byproduct of industrial Sugar production. In experiments, Sugar Beet Pulp was hydrolyzed using five commercial enzymes. A series of shake flask fermentations were conducted using five selected strains of lactic acid bacteria (LAB). The differences in the activities of the enzymes for degrading the principal Sugar Beet Pulp components were reflected in the different yields of total reducing Sugars. The highest yields after hydrolysis and the lowest quantities of insoluble residues were obtained using a mixture (1:1) of Viscozyme® and Ultraflo® Max. In the SHF process, only a portion of the soluble Sugars released by the enzymes from the Sugar Beet Pulp was assimilated by the LAB strains. In SSF, low enzyme loads led to reduction in the efficiency of Sugar accumulation. The risk of carbon catabolic repression was reduced. Our results suggest that SSF has advantages over SHF, including lower processing costs and higher productivity. Lactic acid yield in SSF mode (approx. 30 g/L) was 80-90% higher than that in SHF.
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Integrated Bioethanol Fermentation/Anaerobic Digestion for Valorization of Sugar Beet Pulp
Energies, 2017Co-Authors: Joanna Berlowska, Sebastian Borowski, Maria Balcerek, Katarzyna Pielech-przybylska, Weronika Cieciura, Dorota KregielAbstract:Large amounts of waste biomass are generated in Sugar factories from the processing of Sugar Beets. After diffusion with hot water to draw the Sugar from the Beet pieces, a wet material remains called Pulp. In this study, waste Sugar Beet Pulp biomass was enzymatically depolymerized, and the obtained hydrolyzates were subjected to fermentation processes. Bioethanol, biomethane, and biohydrogen were produced directly from the substrate or in combined mode. Stillage, a distillery by-product, was used as a feedstock for anaerobic digestion. During biosynthesis of ethanol, most of the carbohydrates released from the Sugar Beet Pulp were utilized by a co-culture of Saccharomyces cerevisiae Ethanol Red, and Scheffersomyces stipitis LOCK0047 giving 12.6 g/L of ethanol. Stillage containing unfermented Sugars (mainly arabinose, galactose and raffinose) was found to be a good substrate for methane production (444 dm3 CH4/kg volatile solids (VS)). Better results were achieved with this medium than with enzymatic saccharified biomass. Thermal pre-treatment and adjusting the pH of the inoculum resulted in higher hydrogen production. The largest (p < 0.05) hydrogen yield (252 dm3 H2/kg VS) was achieved with Sugar Beet stillage (SBS). In contrast, without pre-treatment the same medium yielded 35 dm3 H2/kg VS. However, dark fermentation of biohydrogen was more efficient when Sugar Beet Pulp hydrolyzate was used.
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simultaneous saccharification and fermentation of Sugar Beet Pulp for efficient bioethanol production
BioMed Research International, 2016Co-Authors: Joanna Berlowska, Katarzyna Pielechprzybylska, Urszula Dziekonskakubczak, Piotr Patelski, Piotr Dziugan, Maria Balcerek, Dorota KregielAbstract:Sugar Beet Pulp, a byproduct of Sugar Beet processing, can be used as a feedstock in second-generation ethanol production. The objective of this study was to investigate the effects of pretreatment, of the dosage of cellulase and hemicellulase enzyme preparations used, and of aeration on the release of fermentable Sugars and ethanol yield during simultaneous saccharification and fermentation (SSF) of Sugar Beet Pulp-based worts. Pressure-thermal pretreatment was applied to Sugar Beet Pulp suspended in 2% w/w sulphuric acid solution at a ratio providing 12% dry matter. Enzymatic hydrolysis was conducted using Viscozyme and Ultraflo Max (Novozymes) enzyme preparations (0.015–0.02 mL/g dry matter). Two yeast strains were used for fermentation: Ethanol Red (S. cerevisiae) (1 g/L) and Pichia stipitis (0.5 g/L), applied sequentially. The results show that efficient simultaneous saccharification and fermentation of Sugar Beet Pulp was achieved. A 6 h interval for enzymatic activation between the application of enzyme preparations and inoculation with Ethanol Red further improved the fermentation performance, with the highest ethanol concentration reaching g/L and fermentation efficiency relative to the theoretical yield.
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a low cost method for obtaining high value bio based propylene glycol from Sugar Beet Pulp
RSC Advances, 2015Co-Authors: Joanna Berlowska, Halina Kalinowska, Michal Binczarski, Marta Dudkiewicz, I Witonska, A StanishevskyAbstract:A new low-cost pathway for the production of high-value propylene glycol (PG) is proposed. This route of waste biomass utilization employs catalytic reduction of lactic acid obtained from fermented enzymatic digests of Sugar Beet Pulp.
Simon Trakhtenberg - One of the best experts on this subject based on the ideXlab platform.
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Sugar Beet Pulp and apple pomace dietary fibers improve lipid metabolism in rats fed cholesterol
Food Chemistry, 2001Co-Authors: Maria Leontowicz, Elzbieta Bartnikowska, Hanna Leontowicz, G Kulasek, Shela Gorinstein, Simon TrakhtenbergAbstract:The eAect of diets supplemented with Sugar Beet Pulp fiber (SBP, 10%) and apple pomace fiber (AP, 10%) on lipids and lipids peroxides was investigated in 60 male Wistar rats. The rats were divided into six groups of 10 and adapted to cholesterol-free or 0.3% cholesterol diets. The basal diet (BD) contained wheat meal, barley meal, wheat hulls, meat-bone meal, barley sprouts, skimmed milk, fodder yeast, mineral and vitamin mixtures. The Control group (Control) consumed BD only. To the BD were added 3 g/kg cholesterol (Chol), 100 g/kg dry Sugar Beet Pulp fiber (SBP), both 100 g/kg Sugar Beet Pulp fiber and 3 g/kg cholesterol (SBP+Chol), 100 g/kg apple pomace fiber (AP), both 100 g/kg apple pomace fiber and 3 g/kg cholesterol (AP+Chol). The experiment lasted 40 days. Plasma total cholesterol (TC), LDL cholesterol (LDL-C), HDL cholesterol (HDL-C), triglycerides (TG), total phospholipids (TPH), HDL phospholipids (HDL-PH), lipid peroxides (LP) and liver TC concentration were measured. Groups did not diAer before the experiment. In the Chol+SBP and the Chol+AP vs. Chol group the Sugar Beet Pulp and apple pomace dietary fiber supplemented diet significantly (P<0.05) hindered the rise of plasma lipids: (a) TCˇ2.97 vs. 3.69 mmol/l, ˇ20% and 3.01 vs 3.69 mmol/l, ˇ18.4%, respectively; (b) LDL-C ˇ1.36 vs. 2.02 mmol/l, ˇ32.6% and 1.39 vs. 2.02 mmol/l, ˇ31.2%, respectively; (c) TGˇ0.73 vs. 0.88 mmol/l, and 0.75 vs. 0.88 mmol/l;ˇ17 andˇ14.8%, respectively, and TC in liver (17.1 vs. 24.3 mmol/g,ˇ29.6% and 17.9 v. 24.3 mmol/g,ˇ26.3%, respectively. Sugar Beet and apple pomace fiber-supplemented diets significantly hindered the decrease in HDL-PH (0.79 vs. 0.63 mmol/l,ˇ25.3%, P<0.025 and 0.75 vs. 0.63 mmol/l,ˇ19%, P<0.05, respectively) and decreased the level of TPH (1.34 vs. 1.74 mmol/l,ˇ23%, P<0.005 and 1.37 vs. 1.74 mmol/l,ˇ21.3%, P<0.01, respectively). Both Sugar Beet Pulp fiber and apple pomace fiber, in rats fed the basal diet without cholesterol, did not significantly aAect the variables measured. Neither Sugar Beet Pulp fiber or apple pomace fiber-supplemented diets influenced the level of lipid peroxides. These results demonstrate that Sugar Beet Pulp fiber and to a lesser degree apple pomace fiber possess hypolipidemic properties. This is more evident when Sugar Beet Pulp fiber or apple pomace fiber are added to the diet of rats fed cholesterol. The hypolipidemic eAects of both Sugar Beet Pulp fiber and apple pomace fiber can be attributed to their water-soluble parts. The Sugar Beet Pulp and apple pomace fibers have no antioxidant properties. # 2000 Elsevier Science Ltd. All rights reserved.
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Sugar Beet Pulp and apple pomace dietary fibers improve lipid metabolism in rats fed cholesterol
Food Chemistry, 2001Co-Authors: Maria Leontowicz, Elzbieta Bartnikowska, Hanna Leontowicz, G Kulasek, Shela Gorinstein, Simon TrakhtenbergAbstract:The eAect of diets supplemented with Sugar Beet Pulp fiber (SBP, 10%) and apple pomace fiber (AP, 10%) on lipids and lipids peroxides was investigated in 60 male Wistar rats. The rats were divided into six groups of 10 and adapted to cholesterol-free or 0.3% cholesterol diets. The basal diet (BD) contained wheat meal, barley meal, wheat hulls, meat-bone meal, barley sprouts, skimmed milk, fodder yeast, mineral and vitamin mixtures. The Control group (Control) consumed BD only. To the BD were added 3 g/kg cholesterol (Chol), 100 g/kg dry Sugar Beet Pulp fiber (SBP), both 100 g/kg Sugar Beet Pulp fiber and 3 g/kg cholesterol (SBP+Chol), 100 g/kg apple pomace fiber (AP), both 100 g/kg apple pomace fiber and 3 g/kg cholesterol (AP+Chol). The experiment lasted 40 days. Plasma total cholesterol (TC), LDL cholesterol (LDL-C), HDL cholesterol (HDL-C), triglycerides (TG), total phospholipids (TPH), HDL phospholipids (HDL-PH), lipid peroxides (LP) and liver TC concentration were measured. Groups did not diAer before the experiment. In the Chol+SBP and the Chol+AP vs. Chol group the Sugar Beet Pulp and apple pomace dietary fiber supplemented diet significantly (P
