The Experts below are selected from a list of 2796 Experts worldwide ranked by ideXlab platform
Silvia Licoccia - One of the best experts on this subject based on the ideXlab platform.
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layered tetratitanate intercalating Sulfanilic Acid for organic inorganic proton conductors
Solid State Ionics, 2012Co-Authors: Catia De Bonis, Barbara Mecheri, Enrico Traversa, Masaru Miyayama, Ana C. Tavares, Alessandra Depifanio, Silvia LicocciaAbstract:Abstract A hydrous layered tetratitanate derivative intercalating Sulfanilic Acid (SA-H 2 Ti 4 O 9 ) to be used as filler in proton conducting nanocomposite membranes for fuel cell applications was synthesized and characterized. The morphology, composition and structural features of the organic/inorganic compound were investigated by field emission scanning electron microscopy (FE-SEM), elemental analysis, X-ray diffraction (XRD), Fourier transform infrared (FT-IR) spectroscopy, and its thermal stability was tested by thermogravimetric and differential thermal analysis (TG/DTA). The effects caused by the presence of the organic guest molecules bearing sulfonic Acid groups on the proton conductivity of the material were investigated. Conductivity was measured by electrochemical impedance spectroscopy (EIS) while water mobility was tested by dynamic vapour sorption (DVS) measurements. The higher conductivity shown by SA-H 2 Ti 4 O 9 with respect to that of H 2 Ti 4 O 9 is correlated to an increase of both the amount of proton-donor groups and water mobility due to the intercalation of Sulfanilic Acid. Sulfonated polyetheretherketone (SPEEK)-based composite membranes containing different amount of SA-H 2 Ti 4 O 9 were prepared and characterized in terms of microstructural (FE-SEM) features and proton conductivity (EIS). The composite membranes showed higher conductivity values than those of pure SPEEK due to the presence of the organically-modified two-dimensional filler.
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Layered tetratitanate intercalating Sulfanilic Acid for organic/inorganic proton conductors
Solid State Ionics, 2012Co-Authors: Catia De Bonis, Alessandra D'epifanio, Barbara Mecheri, Enrico Traversa, Masaru Miyayama, Ana C. Tavares, Silvia LicocciaAbstract:Abstract A hydrous layered tetratitanate derivative intercalating Sulfanilic Acid (SA-H 2 Ti 4 O 9 ) to be used as filler in proton conducting nanocomposite membranes for fuel cell applications was synthesized and characterized. The morphology, composition and structural features of the organic/inorganic compound were investigated by field emission scanning electron microscopy (FE-SEM), elemental analysis, X-ray diffraction (XRD), Fourier transform infrared (FT-IR) spectroscopy, and its thermal stability was tested by thermogravimetric and differential thermal analysis (TG/DTA). The effects caused by the presence of the organic guest molecules bearing sulfonic Acid groups on the proton conductivity of the material were investigated. Conductivity was measured by electrochemical impedance spectroscopy (EIS) while water mobility was tested by dynamic vapour sorption (DVS) measurements. The higher conductivity shown by SA-H 2 Ti 4 O 9 with respect to that of H 2 Ti 4 O 9 is correlated to an increase of both the amount of proton-donor groups and water mobility due to the intercalation of Sulfanilic Acid. Sulfonated polyetheretherketone (SPEEK)-based composite membranes containing different amount of SA-H 2 Ti 4 O 9 were prepared and characterized in terms of microstructural (FE-SEM) features and proton conductivity (EIS). The composite membranes showed higher conductivity values than those of pure SPEEK due to the presence of the organically-modified two-dimensional filler.
Gábor Rákhely - One of the best experts on this subject based on the ideXlab platform.
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Starvation- and xenobiotic-related transcriptomic responses of the Sulfanilic Acid-degrading bacterium, Novosphingobium resinovorum SA1.
Applied microbiology and biotechnology, 2017Co-Authors: Botond Hegedüs, Katalin Perei, Péter B. Kós, Gábor Bende, Naila Bounedjoum, Gergely Maróti, Krisztián Laczi, Márk Szuhaj, Gábor RákhelyAbstract:Novosphingobium resinovorum SA1 was the first single isolate capable of degrading Sulfanilic Acid, a widely used representative of sulfonated aromatic compounds. The genome of the strain was recently sequenced, and here, we present whole-cell transcriptome analyses of cells exposed to Sulfanilic Acid as compared to cells grown on glucose. The comparison of the transcript profiles suggested that the primary impact of Sulfanilic Acid on the cell transcriptome was a starvation-like effect. The genes of the peripheral, central, and common pathways of Sulfanilic Acid biodegradation had distinct transcript profiles. The peripheral genes located on a plasmid had very high basal expressions which were hardly upregulated by Sulfanilic Acid. The genomic context and the codon usage preference of these genes suggested that they were acquired by horizontal gene transfer. The genes of the central pathways were remarkably inducible by Sulfanilic Acid indicating the presence of a substrate-specific regulatory system in the cells. Surprisingly, the genes of the common part of the metabolic pathway had low and Sulfanilic Acid-independent transcript levels. The approach applied resulted in the identification of the genes of proteins involved in auxiliary processes such as electron transfer, substrate and iron transports, sulfite oxidases, and sulfite transporters. The whole transcriptome analysis revealed that the cells exposed to xenobiotics had multiple responses including general starvation-like, substrate-specific, and substrate-related effects. From the results, we propose that the genes of the peripheral, central, and common parts of the pathway have been evolved independently.
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Complete genome sequence of Novosphingobium resinovorum SA1, a versatile xenobiotic-degrading bacterium capable of utilizing Sulfanilic Acid.
Journal of biotechnology, 2016Co-Authors: Botond Hegedűs, Katalin Perei, Péter B. Kós, Gergely Maróti, Balázs Bálint, Han Ming Gan, Gábor RákhelyAbstract:Sulfanilic Acid (4-aminobenzenesulfonic Acid) is a sulfonated aromatic amine widely used in chemical industries for synthesis of various organic dyes and sulfa drugs. There are quite a few microbial co-cultures or single isolates capable of completely degrading this compound. Novosphingobium resinovorum SA1 was the first single bacterium which could utilize Sulfanilic Acid as its sole carbon, nitrogen and sulfur source. The strain has versatile catabolic routes for the bioconversion of numerous other aromatic compounds. Here, the complete genome sequence of the N. resinovorum SA1 strain is reported. The genome consists of a circular chromosome of 3.8 Mbp and four extrachromosomal elements between 67 and 1 759.8 kbp in size. Three alternative 3-ketoadipate pathways were identified on the plasmids. Sulfanilic Acid is decomposed via a modified 3-ketoadipate pathway and the oxygenases involved form a phylogenetically separate branch on the tree. Sequence analysis of these elements might provide a genetic background for deeper insight into the versatile catabolic metabolism of various aromatic xenobiotics, including Sulfanilic Acid and its derivatives. Moreover, this is also a good model strain for understanding the role and evolution of multiple genetic elements within a single strain.
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Biodegradation of Sulfanilic Acid by Pseudomonas paucimobilis.
Applied microbiology and biotechnology, 2001Co-Authors: Katalin Perei, Gábor Rákhely, Ibolya Kiss, B. Polyák, Kornél L. KovácsAbstract:An aerobic bacterium, isolated from a contaminated site, was able to degrade Sulfanilic Acid (4-aminobenzenesulfonic Acid) and was identified as Pseudomonas paucimobilis. The isolate could grow on Sulfanilic Acid (SA) as its sole carbon and nitrogen source and metabolized the target compound to biomass. The bioconversion capacity depended on the Sulfanilic Acid concentration; greater than 98% elimination of the hazardous compound was achieved at low (10 mM) Sulfanilic Acid concentration, and the yield was greater than 70% at 50 mM concentration of the contaminant. The maximum conversion rate was 1.5 mmol Sulfanilic Acid/h per mg wet cells at 30 °C. Ca-alginate-phytagel proved a good matrix for immobilization of P. paucimobilis, with essentially unaltered biodegradation activity. Removal of Sulfanilic Acid from contaminated industrial waste water was demonstrated. SDS-PAGE analysis of the crude extract revealed novel proteins appearing upon induction with Sulfanilic Acid and related compounds, which indicated alternative degradation mechanisms involving various inducible enzymes.
Catia De Bonis - One of the best experts on this subject based on the ideXlab platform.
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layered tetratitanate intercalating Sulfanilic Acid for organic inorganic proton conductors
Solid State Ionics, 2012Co-Authors: Catia De Bonis, Barbara Mecheri, Enrico Traversa, Masaru Miyayama, Ana C. Tavares, Alessandra Depifanio, Silvia LicocciaAbstract:Abstract A hydrous layered tetratitanate derivative intercalating Sulfanilic Acid (SA-H 2 Ti 4 O 9 ) to be used as filler in proton conducting nanocomposite membranes for fuel cell applications was synthesized and characterized. The morphology, composition and structural features of the organic/inorganic compound were investigated by field emission scanning electron microscopy (FE-SEM), elemental analysis, X-ray diffraction (XRD), Fourier transform infrared (FT-IR) spectroscopy, and its thermal stability was tested by thermogravimetric and differential thermal analysis (TG/DTA). The effects caused by the presence of the organic guest molecules bearing sulfonic Acid groups on the proton conductivity of the material were investigated. Conductivity was measured by electrochemical impedance spectroscopy (EIS) while water mobility was tested by dynamic vapour sorption (DVS) measurements. The higher conductivity shown by SA-H 2 Ti 4 O 9 with respect to that of H 2 Ti 4 O 9 is correlated to an increase of both the amount of proton-donor groups and water mobility due to the intercalation of Sulfanilic Acid. Sulfonated polyetheretherketone (SPEEK)-based composite membranes containing different amount of SA-H 2 Ti 4 O 9 were prepared and characterized in terms of microstructural (FE-SEM) features and proton conductivity (EIS). The composite membranes showed higher conductivity values than those of pure SPEEK due to the presence of the organically-modified two-dimensional filler.
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Layered tetratitanate intercalating Sulfanilic Acid for organic/inorganic proton conductors
Solid State Ionics, 2012Co-Authors: Catia De Bonis, Alessandra D'epifanio, Barbara Mecheri, Enrico Traversa, Masaru Miyayama, Ana C. Tavares, Silvia LicocciaAbstract:Abstract A hydrous layered tetratitanate derivative intercalating Sulfanilic Acid (SA-H 2 Ti 4 O 9 ) to be used as filler in proton conducting nanocomposite membranes for fuel cell applications was synthesized and characterized. The morphology, composition and structural features of the organic/inorganic compound were investigated by field emission scanning electron microscopy (FE-SEM), elemental analysis, X-ray diffraction (XRD), Fourier transform infrared (FT-IR) spectroscopy, and its thermal stability was tested by thermogravimetric and differential thermal analysis (TG/DTA). The effects caused by the presence of the organic guest molecules bearing sulfonic Acid groups on the proton conductivity of the material were investigated. Conductivity was measured by electrochemical impedance spectroscopy (EIS) while water mobility was tested by dynamic vapour sorption (DVS) measurements. The higher conductivity shown by SA-H 2 Ti 4 O 9 with respect to that of H 2 Ti 4 O 9 is correlated to an increase of both the amount of proton-donor groups and water mobility due to the intercalation of Sulfanilic Acid. Sulfonated polyetheretherketone (SPEEK)-based composite membranes containing different amount of SA-H 2 Ti 4 O 9 were prepared and characterized in terms of microstructural (FE-SEM) features and proton conductivity (EIS). The composite membranes showed higher conductivity values than those of pure SPEEK due to the presence of the organically-modified two-dimensional filler.
Nan Gao - One of the best experts on this subject based on the ideXlab platform.
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Silver nanoparticles modified with Sulfanilic Acid for one-step colorimetric and visual determination of histidine in serum
Microchimica Acta, 2016Co-Authors: Pengcheng Huang, Juan Song, Nan GaoAbstract:The authors demonstrate a single-step histidine assay that is based on the use of silver nanoparticles modified with Sulfanilic Acid (SAA-AgNPs). The presence of histidine leads to a visually detectable gradual color change from bright yellow via orange to purple. The effect is assumed to be mainly due to strong π-π stacking, electrostatic interaction, and hydrogen bonding between SAA and histidine. The assay has a 52.7 nM detection limit and works in the 0 to 3.5 μM concentration range. It is selective over other compounds when using appropriate masking agents. The method has been successfully applied to the colorimetric determination of histidine in (spiked) serum samples. Recoveries ranged between 97 % and 107 %, and relative standard deviations are
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silver nanoparticles modified with Sulfanilic Acid for one step colorimetric and visual determination of histidine in serum
Mikrochimica Acta, 2016Co-Authors: Pengcheng Huang, Juan Song, Nan GaoAbstract:The authors demonstrate a single-step histidine assay that is based on the use of silver nanoparticles modified with Sulfanilic Acid (SAA-AgNPs). The presence of histidine leads to a visually detectable gradual color change from bright yellow via orange to purple. The effect is assumed to be mainly due to strong π-π stacking, electrostatic interaction, and hydrogen bonding between SAA and histidine. The assay has a 52.7 nM detection limit and works in the 0 to 3.5 μM concentration range. It is selective over other compounds when using appropriate masking agents. The method has been successfully applied to the colorimetric determination of histidine in (spiked) serum samples. Recoveries ranged between 97 % and 107 %, and relative standard deviations are <0.92 % (for n = 3). The method was also applied to detect polyhistidine-tagged cysteine (His-His-His-His-His-His-Cys) which can be quantified in concentrations down to 5.57 nM. This finding links the method to His-tag technology.
Juan Song - One of the best experts on this subject based on the ideXlab platform.
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Silver nanoparticles modified with Sulfanilic Acid for one-step colorimetric and visual determination of histidine in serum
Microchimica Acta, 2016Co-Authors: Pengcheng Huang, Juan Song, Nan GaoAbstract:The authors demonstrate a single-step histidine assay that is based on the use of silver nanoparticles modified with Sulfanilic Acid (SAA-AgNPs). The presence of histidine leads to a visually detectable gradual color change from bright yellow via orange to purple. The effect is assumed to be mainly due to strong π-π stacking, electrostatic interaction, and hydrogen bonding between SAA and histidine. The assay has a 52.7 nM detection limit and works in the 0 to 3.5 μM concentration range. It is selective over other compounds when using appropriate masking agents. The method has been successfully applied to the colorimetric determination of histidine in (spiked) serum samples. Recoveries ranged between 97 % and 107 %, and relative standard deviations are
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silver nanoparticles modified with Sulfanilic Acid for one step colorimetric and visual determination of histidine in serum
Mikrochimica Acta, 2016Co-Authors: Pengcheng Huang, Juan Song, Nan GaoAbstract:The authors demonstrate a single-step histidine assay that is based on the use of silver nanoparticles modified with Sulfanilic Acid (SAA-AgNPs). The presence of histidine leads to a visually detectable gradual color change from bright yellow via orange to purple. The effect is assumed to be mainly due to strong π-π stacking, electrostatic interaction, and hydrogen bonding between SAA and histidine. The assay has a 52.7 nM detection limit and works in the 0 to 3.5 μM concentration range. It is selective over other compounds when using appropriate masking agents. The method has been successfully applied to the colorimetric determination of histidine in (spiked) serum samples. Recoveries ranged between 97 % and 107 %, and relative standard deviations are <0.92 % (for n = 3). The method was also applied to detect polyhistidine-tagged cysteine (His-His-His-His-His-His-Cys) which can be quantified in concentrations down to 5.57 nM. This finding links the method to His-tag technology.
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Colorimetric detection of melamine in pretreated milk using silver nanoparticles functionalized with Sulfanilic Acid
Food Control, 2015Co-Authors: Juan Song, Yiqun WanAbstract:Abstract Multiple methods have been published to detect melamine, only a few offer sensitivities below 50 nM with complicated procedures and sophisticated equipments. We demonstrate here a simple, rapid and lower-cost assay with high sensitivity for the melamine detection in milk samples using Sulfanilic Acid-modified silver nanoparticles (SAA-AgNPs). Due to the special chemical structures, SAA shows similar response to its analogues, which reveals that the selectivity and sensitivity of SAA itself is poor. However, the formation of SAA-AgNPs dramatically improves the selectivity of SAA and only melamine reacts with SAA-AgNPs. The possible mechanism is discussed. The interaction between exocyclic amine of melamine and SAA induces rapid aggregation of SAA-AgNPs accompanied by a naked-eye visible color change, resulting in precise quantification of melamine that can be monitored by a simple UV-visible spectrometer. Metal ions, amino Acids and sugars that are common in milk have negligible interference influences. The extinction ratio (A 620nm /A 390nm ) is correlated with the melamine concentration over the range of 0.1–3.1 μM. The detection limit is 10.6 nM, which is much lower than the safety limits (8 μM for infant formula in China, 20 μM in both the USA and EU and 1.2 μM in the CAC review for melamine in liquid infant formula). The method is applied successfully to determine melamine in pretreated milk products, indicating the potential practical use for the products suspected of melamine exposure.
