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Elissa Doty - One of the best experts on this subject based on the ideXlab platform.
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expanded polytetrafluoroethylene but not oxidized regenerated cellulose prevents adhesion formation and reformation in a mouse uterine horn model of Surgical Injury
Fertility and Sterility, 1993Co-Authors: A F Haney, Elissa DotyAbstract:Objective To evaluate the ability of the two currently available Surgical barriers, oxidized regenerated cellulose and expanded-polytetrafluoroethylene (PTFE), to prevent postSurgical adhesions. Design Murine uterine horns were approximated in the midline and the contacting uterine surfaces injured by electrocautery, cutting, and scratching, with and without barriers interposed. Sham-operated and experimental animals had adhesions assessed visually and histologically 7days postoperatively. In another group, adhesions were created and then lysed 7days later with barriers interposed. Readhesion formation was assessed 14days after lysis with the PTFE being removed 7days after lysis. Setting Research laboratory Results Adhesions occurred at 58.5% of the electrocautery sites without barriers, 100% of the readhesion sites with recautery for hemostasis, and 92% of the recautery sites without hemostasis. None of the sham-operated sites developed adhesions. When oxidized regenerated cellulose was interposed, adhesions were observed at 36% of uninjured uterine horn sites, 62% with single and 92% with double electrocautery injuries and 90% of the reformation sites. The PTFE did not cause adhesions in uninjured controls and completely prevented adhesion formation and reformation, regardless of the type of Injury or whether hemostasis was achieved. A thin cellular membrane, continuous with the uterine serosa, enveloped the PTFE. Conclusions Expanded-polytetrafluoroethylene, but not oxidized regenerated cellulose, prevents adhesion formation and reformation in this murine uterine horn model. Additionally, oxidized regenerated cellulose was adhesiogenic even without Surgical Injury.
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Reproductive animal researchExpanded-polytetrafluoroethylene but not oxidized regenerated cellulose prevents adhesion formation and reformation in a mouse uterine horn model of Surgical Injury*
Fertility and Sterility, 1993Co-Authors: Elissa DotyAbstract:Objective To evaluate the ability of the two currently available Surgical barriers, oxidized regenerated cellulose and expanded-polytetrafluoroethylene (PTFE), to prevent postSurgical adhesions. Design Murine uterine horns were approximated in the midline and the contacting uterine surfaces injured by electrocautery, cutting, and scratching, with and without barriers interposed. Sham-operated and experimental animals had adhesions assessed visually and histologically 7days postoperatively. In another group, adhesions were created and then lysed 7days later with barriers interposed. Readhesion formation was assessed 14days after lysis with the PTFE being removed 7days after lysis. Setting Research laboratory Results Adhesions occurred at 58.5% of the electrocautery sites without barriers, 100% of the readhesion sites with recautery for hemostasis, and 92% of the recautery sites without hemostasis. None of the sham-operated sites developed adhesions. When oxidized regenerated cellulose was interposed, adhesions were observed at 36% of uninjured uterine horn sites, 62% with single and 92% with double electrocautery injuries and 90% of the reformation sites. The PTFE did not cause adhesions in uninjured controls and completely prevented adhesion formation and reformation, regardless of the type of Injury or whether hemostasis was achieved. A thin cellular membrane, continuous with the uterine serosa, enveloped the PTFE. Conclusions Expanded-polytetrafluoroethylene, but not oxidized regenerated cellulose, prevents adhesion formation and reformation in this murine uterine horn model. Additionally, oxidized regenerated cellulose was adhesiogenic even without Surgical Injury.
Catherine Seager - One of the best experts on this subject based on the ideXlab platform.
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mode of Surgical Injury influences the source of urothelial progenitors during bladder defect repair
Stem cell reports, 2017Co-Authors: Frank Mattias Schafer, Khalid Algarrahi, Alyssa Savarino, Xuehui Yang, Catherine SeagerAbstract:The bladder urothelium functions as a urine-blood barrier and consists of basal, intermediate, and superficial cell populations. Reconstructive procedures such as augmentation cystoplasty and focal mucosal resection involve localized Surgical damage to the bladder wall whereby focal segments of the urothelium and underlying submucosa are respectively removed or replaced and regeneration ensues. We demonstrate using lineage-tracing systems that urothelial regeneration following augmentation cystoplasty with acellular grafts exclusively depends on host keratin 5-expressing basal cells to repopulate all lineages of the de novo urothelium at implant sites. Conversely, repair of focal mucosal defects not only employs this mechanism, but in parallel host intermediate cell daughters expressing uroplakin 2 give rise to themselves and are also contributors to superficial cells in neotissues. These results highlight the diversity of urothelial regenerative responses to Surgical Injury and may lead to advancements in bladder tissue engineering approaches.
Chantal Pichon - One of the best experts on this subject based on the ideXlab platform.
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Enhanced Achilles tendon healing by fibromodulin gene transfer
Nanomedicine: Nanotechnology Biology and Medicine, 2015Co-Authors: Anthony Delalande, Marie-pierre Gosselin, Arnaud Suwalski, William Guilmain, Chloé Leduc, Mathieu Berchel, Paul-alain Jaffres, Patrick Baril, Patrick Midoux, Chantal PichonAbstract:Tendon Injury is a major musculoskeletal disorder with a high public health impact. We propose a non-viral based strategy of gene therapy for the treatment of tendon injuries using histidylated vectors. Gene delivery of fibromodulin, a proteoglycan involved in collagen assembly was found to promote rat Achilles tendon repair in vivo and in vitro. In vivo liposome-based transfection of fibromodulin led to a better healing after Surgical Injury, biomechanical properties were better restored compared to untransfected control. These measures were confirmed by histological observations and scoring. To get better understandings of the mechanisms underlying fibromodulin transfection, an in vitro tendon healing model was developed. In vitro, polymer-based transfection of fibromodulin led to the best wound enclosure speed and a pronounced migration of tenocytes primary cultures was observed. These results suggest that fibromodulin non-viral gene therapy could be proposed as a new therapeutic strategy to accelerate tendon healing.
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enhanced achilles tendon healing by fibromodulin gene transfer
Nanomedicine: Nanotechnology Biology and Medicine, 2015Co-Authors: Anthony Delalande, Marie-pierre Gosselin, Arnaud Suwalski, William Guilmain, Chloé Leduc, Mathieu Berchel, Paul-alain Jaffres, Patrick Baril, Patrick Midoux, Chantal PichonAbstract:Abstract Tendon Injury is a major musculoskeletal disorder with a high public health impact. We propose a non-viral based strategy of gene therapy for the treatment of tendon injuries using histidylated vectors. Gene delivery of fibromodulin, a proteoglycan involved in collagen assembly was found to promote rat Achilles tendon repair in vivo and in vitro. In vivo liposome-based transfection of fibromodulin led to a better healing after Surgical Injury, biomechanical properties were better restored compared to untransfected control. These measures were confirmed by histological observations and scoring. To get better understandings of the mechanisms underlying fibromodulin transfection, an in vitro tendon healing model was developed. In vitro , polymer-based transfection of fibromodulin led to the best wound enclosure speed and a pronounced migration of tenocytes primary cultures was observed. These results suggest that fibromodulin non-viral gene therapy could be proposed as a new therapeutic strategy to accelerate tendon healing. From the Clinical Editor Tendon Injury is relatively common and healing remains unsatisfactory. In this study, the effects of liposomal-based delivery of fibromodulin gene were investigated in a rat Achilles tendon Injury model. The positive results observed would provide a new therapeutic strategy in clinical setting in the future.
Keith C Ozaki - One of the best experts on this subject based on the ideXlab platform.
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Surgical Injury induces local and distant adipose tissue browning
Adipocyte, 2016Co-Authors: Alban Longchamp, Ming Tao, Alexander Bartelt, Kui Ding, Lydia Lynch, Christopher Hine, Jeanmarc Corpataux, Bruce S Kristal, James R Mitchell, Keith C OzakiAbstract:The adipose organ, which comprises brown, white and beige adipocytes, possesses remarkable plasticity in response to feeding and cold exposure. The development of beige adipocytes in white adipose tissue (WAT), a process called browning, represents a promising route to treat metabolic disorders. While Surgical procedures constantly traumatize adipose tissue, its impact on adipocyte phenotype remains to be established. Herein, we studied the effect of trauma on adipocyte phenotype one day after sham, incision control, or Surgical Injury to the left inguinal adipose compartment. Caloric restriction was used to control for surgery-associated body temperature changes and weight loss. We characterized the trauma-induced cellular and molecular changes in subcutaneous, visceral, interscapular, and perivascular adipose tissue using histology, immunohistochemistry, gene expression, and flow cytometry analysis. After one day, Surgical trauma stimulated adipose tissue browning at the site of Injury and, importantly, in the contralateral inguinal depot. Browning was not present after incision only, and was largely independent of surgery-associated body temperature and weight loss. Adipose trauma rapidly recruited monocytes to the injured site and promoted alternatively activated macrophages. Conversely, PDGF receptor-positive beige progenitors were reduced. In this study, we identify adipose trauma as an unexpected driver of selected local and remote adipose tissue browning, holding important implications for the biologic response to Surgical Injury.
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preoperative dietary restriction reduces intimal hyperplasia and protects from ischemia reperfusion Injury
Journal of Vascular Surgery, 2016Co-Authors: Christine R Mauro, Alban Longchamp, Bruce S Kristal, Keith C Ozaki, Peng Yu, Humberto J Trevinovillerreal, James R MitchellAbstract:Objective Whereas chronic overnutrition is a risk factor for Surgical complications, long-term dietary restriction (reduced food intake without malnutrition) protects in preclinical models of Surgical stress. Building on the emerging concept that acute preoperative dietary perturbations can affect the body's response to Surgical stress, we hypothesized that short-term high-fat diet (HFD) feeding before surgery is detrimental, whereas short-term nutrient/energy restriction before surgery can reverse negative outcomes. We tested this hypothesis in two distinct murine models of vascular Surgical Injury, ischemia-reperfusion (IR) and intimal hyperplasia (IH). Methods Short-term overnutrition was achieved by feeding mice a HFD consisting of 60% calories from fat for 2 weeks. Short-term dietary restriction consisted of either 1 week of restricted access to a protein-free diet (protein/energy restriction) or 3 days of water-only fasting immediately before surgery; after surgery, all mice were given ad libitum access to a complete diet. To assess the impact of preoperative nutrition on Surgical outcome, mice were challenged in one of two fundamentally distinct Surgical Injury models: IR Injury to either kidney or liver, or a carotid focal stenosis model of IH. Results Three days of fasting or 1 week of preoperative protein/energy restriction attenuated IH development measured 28 days after focal carotid stenosis. One week of preoperative protein/energy restriction also reduced plasma urea, creatinine, and damage to the corticomedullary junction after renal IR and decreased aspartate transaminase, alanine transaminase, and hemorrhagic necrosis after hepatic IR. However, exposure to a HFD for 2 weeks before surgery had no significant impact on kidney or hepatic function after IR or IH after focal carotid stenosis. Conclusions Short-term dietary restriction immediately before surgery significantly attenuated the vascular wall hyperplastic response and improved IR outcome. The findings suggest plasticity in the body's response to these vascular Surgical injuries that can be manipulated by novel yet practical preoperative dietary interventions.
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lack of interleukin 1 signaling results in perturbed early vein graft wall adaptations
Surgery, 2013Co-Authors: Binh T Nguyen, Ming Tao, Christine R Mauro, Tianyu Jiang, Yuqi Wang, Keith C OzakiAbstract:Background Vein grafts fail as the result of wall maladaptations to Surgical Injury and hemodynamic perturbations. Interleukin-1 signaling has emerged as an important mediator of the vascular response to trauma and hemodynamically induced vascular lesions. We therefore hypothesized that interleukin-1 signaling drives early vein graft wall adaptations. Methods Using interleukin-1 type I receptor knockout (IL-1RI−/−) and wild-type (B6129SF2/J) mice, we investigated morphologic changes 28 days after interposition isograft from donor inferior vena cava to recipient carotid artery, without (n = 19) or with (n = 13) outflow restriction. The impact of mouse strain on the response to vein arterialization also was evaluated between B6129SF2/J (n = 18) and C57BL/6J (n = 19) mice. Results No differences were observed in the traditional end points of intimal thickness and calculated luminal area, yet media+adventitia thickness of the vein graft wall of IL-1RI−/− mice was 44% to 52% less than wild-type mice, at the both proximal (P Conclusion When lacking IL-1 signaling, the vein graft wall adapts differently compared with the injured artery, showing typical intima hyperplasia although attenuated media+adventitia thickening. B6129SF2/J mice exhibit more media+adventitia response than C57BL/6J mice. The inflammatory networks that underlie the vein response to arterialization hold many roles in the adaptation of the total wall; thus, the utility of anti-inflammatory approaches to extend the durability of vein grafts comes into question.
Tsang, Albert Siu Hong - One of the best experts on this subject based on the ideXlab platform.
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Experimental Studies in Tendinopathy and The Effects of Mesenchymal Stem Cells on Healing: An Ovine Model
School of Veterinary Science, 2020Co-Authors: Tsang, Albert Siu HongAbstract:Tendon injuries are a major problem in athletic horses and represent the most common form of musculoskeletal Injury. Recurrent Injury is common and re-Injury rates of up to 53% have been demonstrated within 3 years. Historically, clinical treatments have limited efficacy; producing suboptimal results and not reducing re-Injury risk. Stem cells have been injected into clinical and experimental equine superficial digital flexor tendon (SDFT) injuries with encouraging results. The aims of this thesis were to investigate the possible adverse effects that localised tendon Injury could have on adjacent, regional tendons, as a potential cause of re-Injury, and to determine the optimal time for implantation of mesenchymal stem cells following Injury to enhance tendon healing. The effects of these interventions were assessed through biomechanical, histopathological and biomolecular analyses on an ovine model of tendinopathy. Focal Surgical Injury to the SDFT resulted in a mild tendinopathy within the deep digital flexor tendon eight weeks after the initial Injury. These changes appeared to resolve over time, with improvements in histological and gene expression parameters, with no significant biomechanical effects observed at 12 and 26 weeks. Complete transection of the extensor tendons did not affect the health and function of either flexor tendon at eight weeks. Treatment of focal Surgical Injury of the SDFT with a single injection of allogeneic bone marrow-derived mesenchymal stem cells at two weeks resulted in improvements in histological, gene expression and biomechanical parameters at 26 weeks. Alternatively, treatment with mesenchymal stem cells at eight weeks showed no significant differences compared with operated controls. The results of this study suggest that early therapy with stem cells following Injury is likely to enhance healing and provide a better outcome.Access is restricted to staff and students of the University of Sydney . UniKey credentials are required. Non university access may be obtained by visiting the University of Sydney Library
