The Experts below are selected from a list of 210 Experts worldwide ranked by ideXlab platform
Manuel González-guerrero - One of the best experts on this subject based on the ideXlab platform.
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Medicago truncatula copper transporter 1 (MtCOPT1) delivers copper for Symbiotic Nitrogen Fixation
The New phytologist, 2018Co-Authors: Marta Senovilla, Michael K. Udvardi, Viviana Escudero, Isidro Abreu, Rosario Castro-rodríguez, Igor S. Kryvoruchko, Juan Imperial, Manuel González-guerreroAbstract:Copper is an essential nutrient for Symbiotic Nitrogen Fixation. This element is delivered by the host plant to the nodule, where membrane copper (Cu) transporter would introduce it into the cell to synthesize cupro-proteins. COPT family members in the model legume Medicago truncatula were identified and their expression determined. Yeast complementation assays, confocal microscopy and phenotypical characterization of a Tnt1 insertional mutant line were carried out in the nodule-specific M. truncatula COPT family member. Medicago truncatula genome encodes eight COPT transporters. MtCOPT1 (Medtr4g019870) is the only nodule-specific COPT gene. It is located in the plasma membrane of the differentiation, interzone and early Fixation zones. Loss of MtCOPT1 function results in a Cu-mitigated reduction of biomass production when the plant obtains its Nitrogen exclusively from Symbiotic Nitrogen Fixation. Mutation of MtCOPT1 results in diminished Nitrogenase activity in nodules, likely an indirect effect from the loss of a Cu-dependent function, such as cytochrome oxidase activity in copt1-1 bacteroids. These data are consistent with a model in which MtCOPT1 transports Cu from the apoplast into nodule cells to provide Cu for essential metabolic processes associated with Symbiotic Nitrogen Fixation.
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An Iron-Activated Citrate Transporter, MtMATE67, Is Required for Symbiotic Nitrogen Fixation
Plant physiology, 2017Co-Authors: Igor S. Kryvoruchko, Manuel Tejada-jiménez, Pratyush Routray, Senjuti Sinharoy, Ivone Torres-jerez, Lydia Finney, Jin Nakashima, Catalina I. Pislariu, Vagner A. Benedito, Manuel González-guerreroAbstract:Iron (Fe) is an essential micronutrient for Symbiotic Nitrogen Fixation in legume nodules, where it is required for the activity of bacterial Nitrogenase, plant leghemoglobin, respiratory oxidases, and other Fe proteins in both organisms. Fe solubility and transport within and between plant tissues is facilitated by organic chelators, such as nicotianamine and citrate. We have characterized a nodule-specific citrate transporter of the multidrug and toxic compound extrusion family, MtMATE67 of Medicago truncatula The MtMATE67 gene was induced early during nodule development and expressed primarily in the invasion zone of mature nodules. The MtMATE67 protein was localized to the plasma membrane of nodule cells and also the symbiosome membrane surrounding bacteroids in infected cells. In oocytes, MtMATE67 transported citrate out of cells in an Fe-activated manner. Loss of MtMATE67 gene function resulted in accumulation of Fe in the apoplasm of nodule cells and a substantial decrease in Symbiotic Nitrogen Fixation and plant growth. Taken together, the results point to a primary role of MtMATE67 in citrate efflux from nodule cells in response to an Fe signal. This efflux is necessary to ensure Fe(III) solubility and mobility in the apoplasm and uptake into nodule cells. Likewise, MtMATE67-mediated citrate transport into the symbiosome space would increase the solubility and availability of Fe(III) for rhizobial bacteroids.
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Medicago truncatula copper transporter1 (MtCOPT1) delivers copper for Symbiotic Nitrogen Fixation
2017Co-Authors: Marta Senovilla, Michael K. Udvardi, Viviana Escudero, Isidro Abreu, Rosario Castro-rodríguez, Igor S. Kryvoruchko, Juan Imperial, Manuel González-guerreroAbstract:- Copper is an essential nutrient for Symbiotic Nitrogen Fixation. This element is delivered by the host plant to the nodule, where membrane copper transporter would introduce it into the cell to synthesize cuproproteins. - COPT family members in model legume Medicago truncatula were identified and their expression determined. Yeast complementation assays, confocal microscopy, and phenotypical characterization of a Tnt1 insertional mutant line were carried out in the nodule-specific M. truncatula COPT family member. - Medicago truncatula genome encodes eight COPT transporters. MtCOPT1 (Medtr4g019870) is the only nodule-specific COPT gene. It is located in the plasma membrane of the differentiation, interzone and early Fixation zones. Loss of MtCOPT1 function results in a copper-mitigated reduction of biomass production when the plant obtains its Nitrogen exclusively from Symbiotic Nitrogen Fixation. Mutation of MtCOPT1 results in diminished Nitrogenase activity in nodules, likely an indirect effect from the loss of a copper-dependent function, such as cytochrome oxidase activity in copt1-1 bacteroids. - These data are consistent with a model in which MtCOPT1 transports copper from the apoplast into nodule cells to provide copper for essential metabolic processes associated with Symbiotic Nitrogen Fixation.
Michael K. Udvardi - One of the best experts on this subject based on the ideXlab platform.
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celebrating 20 years of genetic discoveries in legume nodulation and Symbiotic Nitrogen Fixation
The Plant Cell, 2020Co-Authors: Sonali Roy, Kirankumar S. Mysore, Catalina I. Pislariu, Wei Liu, Raja Sekhar Nandety, Ashley D Crook, Julia Frugoli, Rebecca Dickstein, Michael K. UdvardiAbstract:Since 1999, various forward- and reverse-genetic approaches have uncovered nearly 200 genes required for Symbiotic Nitrogen Fixation (SNF) in legumes. These discoveries advanced our understanding of the evolution of SNF in plants and its relationship to other beneficial endosymbioses, signaling between plants and microbes, the control of microbial infection of plant cells, the control of plant cell division leading to nodule development, autoregulation of nodulation, intracellular accommodation of bacteria, nodule oxygen homeostasis, the control of bacteroid differentiation, metabolism and transport supporting symbiosis, and the control of nodule senescence. This review catalogs and contextualizes all of the plant genes currently known to be required for SNF in two model legume species, Medicago truncatula and Lotus japonicus, and two crop species, Glycine max (soybean) and Phaseolus vulgaris (common bean). We also briefly consider the future of SNF genetics in the era of pan-genomics and genome editing.
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Medicago truncatula copper transporter 1 (MtCOPT1) delivers copper for Symbiotic Nitrogen Fixation
The New phytologist, 2018Co-Authors: Marta Senovilla, Michael K. Udvardi, Viviana Escudero, Isidro Abreu, Rosario Castro-rodríguez, Igor S. Kryvoruchko, Juan Imperial, Manuel González-guerreroAbstract:Copper is an essential nutrient for Symbiotic Nitrogen Fixation. This element is delivered by the host plant to the nodule, where membrane copper (Cu) transporter would introduce it into the cell to synthesize cupro-proteins. COPT family members in the model legume Medicago truncatula were identified and their expression determined. Yeast complementation assays, confocal microscopy and phenotypical characterization of a Tnt1 insertional mutant line were carried out in the nodule-specific M. truncatula COPT family member. Medicago truncatula genome encodes eight COPT transporters. MtCOPT1 (Medtr4g019870) is the only nodule-specific COPT gene. It is located in the plasma membrane of the differentiation, interzone and early Fixation zones. Loss of MtCOPT1 function results in a Cu-mitigated reduction of biomass production when the plant obtains its Nitrogen exclusively from Symbiotic Nitrogen Fixation. Mutation of MtCOPT1 results in diminished Nitrogenase activity in nodules, likely an indirect effect from the loss of a Cu-dependent function, such as cytochrome oxidase activity in copt1-1 bacteroids. These data are consistent with a model in which MtCOPT1 transports Cu from the apoplast into nodule cells to provide Cu for essential metabolic processes associated with Symbiotic Nitrogen Fixation.
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Medicago truncatula copper transporter1 (MtCOPT1) delivers copper for Symbiotic Nitrogen Fixation
2017Co-Authors: Marta Senovilla, Michael K. Udvardi, Viviana Escudero, Isidro Abreu, Rosario Castro-rodríguez, Igor S. Kryvoruchko, Juan Imperial, Manuel González-guerreroAbstract:- Copper is an essential nutrient for Symbiotic Nitrogen Fixation. This element is delivered by the host plant to the nodule, where membrane copper transporter would introduce it into the cell to synthesize cuproproteins. - COPT family members in model legume Medicago truncatula were identified and their expression determined. Yeast complementation assays, confocal microscopy, and phenotypical characterization of a Tnt1 insertional mutant line were carried out in the nodule-specific M. truncatula COPT family member. - Medicago truncatula genome encodes eight COPT transporters. MtCOPT1 (Medtr4g019870) is the only nodule-specific COPT gene. It is located in the plasma membrane of the differentiation, interzone and early Fixation zones. Loss of MtCOPT1 function results in a copper-mitigated reduction of biomass production when the plant obtains its Nitrogen exclusively from Symbiotic Nitrogen Fixation. Mutation of MtCOPT1 results in diminished Nitrogenase activity in nodules, likely an indirect effect from the loss of a copper-dependent function, such as cytochrome oxidase activity in copt1-1 bacteroids. - These data are consistent with a model in which MtCOPT1 transports copper from the apoplast into nodule cells to provide copper for essential metabolic processes associated with Symbiotic Nitrogen Fixation.
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Symbiotic Nitrogen Fixation research in the postgenomics era
New Phytologist, 2002Co-Authors: Gillian Colebatch, Ben Trevaskis, Michael K. UdvardiAbstract:Summary Nitrogen-fixing symbioses between legumes and rhizobia are important for sustainable agriculture and contribute significantly to the global Nitrogen cycle. The genomes of two rhizobial species, Mesorhizobium loti and Sinorhizobium meliloti have now been completely sequenced. Hundreds of thousands of expressed sequence tags representing tens of thousands of different genes from three major legume species; soybean, Medicago truncatula and Lotus japonicus have also been deposited in the public domain. Lotus japonicus recently became the focus of a genome project that aims to sequence one third of the entire genome over the next 5 yr. With this as a backdrop, the stage is set for a renaissance in symbiosis research, which will provide new insight into the complex molecular interplay that underpins Symbiotic Nitrogen Fixation. This review considers how functional genomics might contribute to this renaissance.
Viviana Escudero - One of the best experts on this subject based on the ideXlab platform.
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Nicotianamine Synthase 2 Is Required for Symbiotic Nitrogen Fixation in Medicago truncatula Nodules.
Frontiers in plant science, 2020Co-Authors: Viviana Escudero, Isidro Abreu, Eric Del Sastre, Manuel Tejada-jiménez, Camille Larue, Lorena Novoa-aponte, Jiangqi Wen, Kirankumar S. Mysore, Jorge Castillo-gonzalez, Javier AbadíaAbstract:Symbiotic Nitrogen Fixation carried out by the interaction between legumes and diazotrophic bacteria known as rhizobia requires relatively large levels of transition metals. These elements are cofactors of many key enzymes involved in this process. Metallic micronutrients are obtained from soil by the roots and directed to sink organs by the vasculature, in a process mediated by a number of metal transporters and small organic molecules that facilitate metal delivery in the plant fluids. Among the later, nicotianamine is one of the most important. Synthesized by nicotianamine synthases (NAS), this molecule forms metal complexes participating in intracellular metal homeostasis and long-distance metal trafficking. Here we characterized the NAS2 gene from model legume Medicago truncatula. MtNAS2 is located in the root vasculature and in all nodule tissues in the infection and Fixation zones. Symbiotic Nitrogen Fixation requires of MtNAS2 function, as indicated by the loss of Nitrogenase activity in the insertional mutant nas2-1, phenotype reverted by reintroduction of a wild-type copy of MtNAS2. This would result from the altered iron distribution in nas2-1 nodules shown with X-ray fluorescence. Moreover, iron speciation is also affected in these nodules. These data suggest a role of nicotianamine in iron delivery for Symbiotic Nitrogen Fixation.
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Nicotianamine synthase 2 is required for Symbiotic Nitrogen Fixation in Medicago truncatula nodules
2019Co-Authors: Viviana Escudero, Isidro Abreu, Eric Del Sastre, Manuel Tejada-jiménez, Camille Larue, Lorena Novoa-aponte, Jiangqi Wen, Kirankumar S. Mysore, Javier Abadía, José M. ArgüelloAbstract:SUMMARY Symbiotic Nitrogen Fixation carried out by the interaction between legumes and diazotrophic bacteria known as rhizobia requires of relatively large levels of transition metals. These elements act as cofactors of many key enzymes involved in this process. Metallic micronutrients are obtained from soil by the roots and directed to sink organs by the vasculature, in a process participated by a number of metal transporters and small organic molecules that mediate metal delivery in the plant fluids. Among the later, nicotianamine is one of the most important. Synthesized by nicotianamine synthases (NAS), this non-proteinogenic amino acid forms metal complexes participating in intracellular metal homeostasis and long-distance metal trafficking. Here we characterized the NAS2 gene from model legume Medicago truncatula. MtNAS2 is located in the root vasculature and in all nodule tissues in the infection and Fixation zones. Symbiotic Nitrogen Fixation requires of MtNAS2 function, as indicated by the loss of Nitrogenase activity in the insertional mutant nas2-1, a phenotype reverted by reintroduction of a wild-type copy of MtNAS2. This would be the result of the altered iron distribution in nas2-1 nodules, as indicated by X-ray fluorescence studies. Moreover, iron speciation is also affected in these nodules. These data suggest a role of nicotianamine in iron delivery for Symbiotic Nitrogen Fixation. Significance Statement Nicotianamine synthesis mediated by MtNAS2 is important for iron allocation for Symbiotic Nitrogen Fixation by rhizobia in Medicago truncatula root nodules.
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Medicago truncatula copper transporter 1 (MtCOPT1) delivers copper for Symbiotic Nitrogen Fixation
The New phytologist, 2018Co-Authors: Marta Senovilla, Michael K. Udvardi, Viviana Escudero, Isidro Abreu, Rosario Castro-rodríguez, Igor S. Kryvoruchko, Juan Imperial, Manuel González-guerreroAbstract:Copper is an essential nutrient for Symbiotic Nitrogen Fixation. This element is delivered by the host plant to the nodule, where membrane copper (Cu) transporter would introduce it into the cell to synthesize cupro-proteins. COPT family members in the model legume Medicago truncatula were identified and their expression determined. Yeast complementation assays, confocal microscopy and phenotypical characterization of a Tnt1 insertional mutant line were carried out in the nodule-specific M. truncatula COPT family member. Medicago truncatula genome encodes eight COPT transporters. MtCOPT1 (Medtr4g019870) is the only nodule-specific COPT gene. It is located in the plasma membrane of the differentiation, interzone and early Fixation zones. Loss of MtCOPT1 function results in a Cu-mitigated reduction of biomass production when the plant obtains its Nitrogen exclusively from Symbiotic Nitrogen Fixation. Mutation of MtCOPT1 results in diminished Nitrogenase activity in nodules, likely an indirect effect from the loss of a Cu-dependent function, such as cytochrome oxidase activity in copt1-1 bacteroids. These data are consistent with a model in which MtCOPT1 transports Cu from the apoplast into nodule cells to provide Cu for essential metabolic processes associated with Symbiotic Nitrogen Fixation.
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Medicago truncatula copper transporter1 (MtCOPT1) delivers copper for Symbiotic Nitrogen Fixation
2017Co-Authors: Marta Senovilla, Michael K. Udvardi, Viviana Escudero, Isidro Abreu, Rosario Castro-rodríguez, Igor S. Kryvoruchko, Juan Imperial, Manuel González-guerreroAbstract:- Copper is an essential nutrient for Symbiotic Nitrogen Fixation. This element is delivered by the host plant to the nodule, where membrane copper transporter would introduce it into the cell to synthesize cuproproteins. - COPT family members in model legume Medicago truncatula were identified and their expression determined. Yeast complementation assays, confocal microscopy, and phenotypical characterization of a Tnt1 insertional mutant line were carried out in the nodule-specific M. truncatula COPT family member. - Medicago truncatula genome encodes eight COPT transporters. MtCOPT1 (Medtr4g019870) is the only nodule-specific COPT gene. It is located in the plasma membrane of the differentiation, interzone and early Fixation zones. Loss of MtCOPT1 function results in a copper-mitigated reduction of biomass production when the plant obtains its Nitrogen exclusively from Symbiotic Nitrogen Fixation. Mutation of MtCOPT1 results in diminished Nitrogenase activity in nodules, likely an indirect effect from the loss of a copper-dependent function, such as cytochrome oxidase activity in copt1-1 bacteroids. - These data are consistent with a model in which MtCOPT1 transports copper from the apoplast into nodule cells to provide copper for essential metabolic processes associated with Symbiotic Nitrogen Fixation.
J. Nösberger - One of the best experts on this subject based on the ideXlab platform.
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Symbiotic Nitrogen Fixation of White Clover in a Mixed Sward is not Limited by Height of Repeated Cutting
Journal of Agronomy and Crop Science, 1994Co-Authors: Thakshala Seresinhe, U. A. Hartwig, W. Kessler, J. NösbergerAbstract:The aim of this study was to investigate whether Symbiotic Nitrogen Fixation in white clover nodules limits Nitrogen supply and hence clover growth by repeated defoliation at two cutting heights. Other possible factors governing Symbiotic Nitrogen Fixation in the field were also elucidated. Using 15N, a 2-year field experiment including white clover (Trifolium repens L. cv. Ladino) and perennial ryegrass (Loliumperenne L. cv. Bastion) in monocultures and in mixtures was conducted in Eschikon, Switzerland. The effect of two cutting heights (4 cm and 10 cm above ground level) on the performance of Symbiotic Nitrogen Fixation of white clover in the different sward-types was investigated. After each harvest, the plots were fertilized with 3 g N m-2(equivalent to 30 kg N ha-1 cut-1 or 210 kg N ha-1 year-1). In both years, white clover grown in a mixture with grass received a significantly higher percentage of Nitrogen from Symbiotic Fixation compared with clover grown in monoculture. This phenomenon is attributed to the strong competitiveness of ryegrass in soil Nitrogen uptake. Consequently, white clover in the clover-ryegrass mixture was more dependent on Symbiotic Nitrogen Fixation than that grown in monoculture. The cutting height did not preferentially influence Symbiotic Nitrogen Fixation, as opposed to the uptake of mineral Nitrogen from the soil. From this finding it is suggested that Symbiotic Nitrogen Fixation did not limit the supply of Nitrogen to clover and hence its growth. It is proposed that Symbiotic Nitrogen Fixation in white clover is regulated by the demand for Nitrogen rather than by the availability of carbohydrate reserves in the stolons. Symbiotic Nitrogen Fixation should thus be looked upon as an integrated plant growth factor and not as an isolated phenomenon.
Isidro Abreu - One of the best experts on this subject based on the ideXlab platform.
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Nicotianamine Synthase 2 Is Required for Symbiotic Nitrogen Fixation in Medicago truncatula Nodules.
Frontiers in plant science, 2020Co-Authors: Viviana Escudero, Isidro Abreu, Eric Del Sastre, Manuel Tejada-jiménez, Camille Larue, Lorena Novoa-aponte, Jiangqi Wen, Kirankumar S. Mysore, Jorge Castillo-gonzalez, Javier AbadíaAbstract:Symbiotic Nitrogen Fixation carried out by the interaction between legumes and diazotrophic bacteria known as rhizobia requires relatively large levels of transition metals. These elements are cofactors of many key enzymes involved in this process. Metallic micronutrients are obtained from soil by the roots and directed to sink organs by the vasculature, in a process mediated by a number of metal transporters and small organic molecules that facilitate metal delivery in the plant fluids. Among the later, nicotianamine is one of the most important. Synthesized by nicotianamine synthases (NAS), this molecule forms metal complexes participating in intracellular metal homeostasis and long-distance metal trafficking. Here we characterized the NAS2 gene from model legume Medicago truncatula. MtNAS2 is located in the root vasculature and in all nodule tissues in the infection and Fixation zones. Symbiotic Nitrogen Fixation requires of MtNAS2 function, as indicated by the loss of Nitrogenase activity in the insertional mutant nas2-1, phenotype reverted by reintroduction of a wild-type copy of MtNAS2. This would result from the altered iron distribution in nas2-1 nodules shown with X-ray fluorescence. Moreover, iron speciation is also affected in these nodules. These data suggest a role of nicotianamine in iron delivery for Symbiotic Nitrogen Fixation.
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Medicago truncatula Yellow Stripe1-Like3 gene is involved in Symbiotic Nitrogen Fixation
2019Co-Authors: Rosario Castro-rodríguez, Isidro Abreu, Lorena Novoa-aponte, Jiangqi Wen, Kirankumar S. Mysore, Javier Abadía, María Reguera, Ana Mijovilovich, Francisco J. Jiménez-pastor, Ana Álvarez-fernándezAbstract:Symbiotic Nitrogen Fixation carried out in legume root nodules requires transition metals. These nutrients are delivered by the host plant to the endoSymbiotic Nitrogen-fixing bacteria living with the nodule cells, a process in which vascular transport is essential. As occurs in root-to-shoot transport, members of the Yellow Stripe-Like (YSL) family of metal transporters should also be required for root-to-nodule metal delivery. The genome of the model legume Medicago truncatula encodes for eight YSL proteins, four of them with a high degree of similarity to Arabidopsis thaliana YSLs involved in long-distance metal trafficking. Among them, MtYSL3 is a plasma membrane protein expressed by vascular cells in roots and nodules, and by cortical nodule cells. Reducing expression levels of this gene had no major effect on plant physiology when assimilable Nitrogen was provided in the nutrient solution. However, nodule functioning was severely impaired, with a significant reduction of Nitrogen Fixation capabilities. Further, iron and zinc accumulation and distribution changed. Iron was retained in the apical region of the nodule, while zinc became strongly accumulated in the nodule veins in the ysl3 mutant. These data suggest a role of MtYSL3 in vascular delivery of iron and zinc to Symbiotic Nitrogen Fixation.
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Nicotianamine synthase 2 is required for Symbiotic Nitrogen Fixation in Medicago truncatula nodules
2019Co-Authors: Viviana Escudero, Isidro Abreu, Eric Del Sastre, Manuel Tejada-jiménez, Camille Larue, Lorena Novoa-aponte, Jiangqi Wen, Kirankumar S. Mysore, Javier Abadía, José M. ArgüelloAbstract:SUMMARY Symbiotic Nitrogen Fixation carried out by the interaction between legumes and diazotrophic bacteria known as rhizobia requires of relatively large levels of transition metals. These elements act as cofactors of many key enzymes involved in this process. Metallic micronutrients are obtained from soil by the roots and directed to sink organs by the vasculature, in a process participated by a number of metal transporters and small organic molecules that mediate metal delivery in the plant fluids. Among the later, nicotianamine is one of the most important. Synthesized by nicotianamine synthases (NAS), this non-proteinogenic amino acid forms metal complexes participating in intracellular metal homeostasis and long-distance metal trafficking. Here we characterized the NAS2 gene from model legume Medicago truncatula. MtNAS2 is located in the root vasculature and in all nodule tissues in the infection and Fixation zones. Symbiotic Nitrogen Fixation requires of MtNAS2 function, as indicated by the loss of Nitrogenase activity in the insertional mutant nas2-1, a phenotype reverted by reintroduction of a wild-type copy of MtNAS2. This would be the result of the altered iron distribution in nas2-1 nodules, as indicated by X-ray fluorescence studies. Moreover, iron speciation is also affected in these nodules. These data suggest a role of nicotianamine in iron delivery for Symbiotic Nitrogen Fixation. Significance Statement Nicotianamine synthesis mediated by MtNAS2 is important for iron allocation for Symbiotic Nitrogen Fixation by rhizobia in Medicago truncatula root nodules.
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Medicago truncatula copper transporter 1 (MtCOPT1) delivers copper for Symbiotic Nitrogen Fixation
The New phytologist, 2018Co-Authors: Marta Senovilla, Michael K. Udvardi, Viviana Escudero, Isidro Abreu, Rosario Castro-rodríguez, Igor S. Kryvoruchko, Juan Imperial, Manuel González-guerreroAbstract:Copper is an essential nutrient for Symbiotic Nitrogen Fixation. This element is delivered by the host plant to the nodule, where membrane copper (Cu) transporter would introduce it into the cell to synthesize cupro-proteins. COPT family members in the model legume Medicago truncatula were identified and their expression determined. Yeast complementation assays, confocal microscopy and phenotypical characterization of a Tnt1 insertional mutant line were carried out in the nodule-specific M. truncatula COPT family member. Medicago truncatula genome encodes eight COPT transporters. MtCOPT1 (Medtr4g019870) is the only nodule-specific COPT gene. It is located in the plasma membrane of the differentiation, interzone and early Fixation zones. Loss of MtCOPT1 function results in a Cu-mitigated reduction of biomass production when the plant obtains its Nitrogen exclusively from Symbiotic Nitrogen Fixation. Mutation of MtCOPT1 results in diminished Nitrogenase activity in nodules, likely an indirect effect from the loss of a Cu-dependent function, such as cytochrome oxidase activity in copt1-1 bacteroids. These data are consistent with a model in which MtCOPT1 transports Cu from the apoplast into nodule cells to provide Cu for essential metabolic processes associated with Symbiotic Nitrogen Fixation.
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Medicago truncatula copper transporter1 (MtCOPT1) delivers copper for Symbiotic Nitrogen Fixation
2017Co-Authors: Marta Senovilla, Michael K. Udvardi, Viviana Escudero, Isidro Abreu, Rosario Castro-rodríguez, Igor S. Kryvoruchko, Juan Imperial, Manuel González-guerreroAbstract:- Copper is an essential nutrient for Symbiotic Nitrogen Fixation. This element is delivered by the host plant to the nodule, where membrane copper transporter would introduce it into the cell to synthesize cuproproteins. - COPT family members in model legume Medicago truncatula were identified and their expression determined. Yeast complementation assays, confocal microscopy, and phenotypical characterization of a Tnt1 insertional mutant line were carried out in the nodule-specific M. truncatula COPT family member. - Medicago truncatula genome encodes eight COPT transporters. MtCOPT1 (Medtr4g019870) is the only nodule-specific COPT gene. It is located in the plasma membrane of the differentiation, interzone and early Fixation zones. Loss of MtCOPT1 function results in a copper-mitigated reduction of biomass production when the plant obtains its Nitrogen exclusively from Symbiotic Nitrogen Fixation. Mutation of MtCOPT1 results in diminished Nitrogenase activity in nodules, likely an indirect effect from the loss of a copper-dependent function, such as cytochrome oxidase activity in copt1-1 bacteroids. - These data are consistent with a model in which MtCOPT1 transports copper from the apoplast into nodule cells to provide copper for essential metabolic processes associated with Symbiotic Nitrogen Fixation.
