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Ichiro Sekiya - One of the best experts on this subject based on the ideXlab platform.
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Fibrous Synovium Releases Higher Numbers of Mesenchymal Stem Cells Than Adipose Synovium in a Suspended Synovium Culture Model.
Arthroscopy, 2016Co-Authors: Kenta Katagiri, Takeshi Muneta, Yu Matsukura, Nobutake Ozeki, Mitsuru Mizuno, Hisako Katano, Ichiro SekiyaAbstract:Purpose To develop an in vitro model, the "suspended Synovium culture model," to demonstrate the mobilization of mesenchymal stem cells (MSCs) from the Synovium into a noncontacted culture dish through culture medium. In addition, to examine which Synovium, fibrous Synovium or adipose Synovium, released more MSCs in the knee with osteoarthritis. Methods Human synovial tissue was harvested during total knee arthroplasty from knee joints of 34 patients with osteoarthritis (28 patients: only fibrous Synovium, 6 patients: fibrous and adipose Synovium). One gram of Synovium was suspended with a thread in a bottle containing 40 mL of culture medium and a 3.5-cm-diameter culture dish at the bottom. After 7 days, the culture dish in the bottle was examined. For the cells harvested, multipotentiality and surface epitopes were analyzed. The numbers of colonies derived from fibrous Synovium and adipose Synovium were also compared. Results Colonies of spindle-shaped cells were observed in the culture dish in all 28 donors. Colonies numbered 26 on average, and the cells derived from colony-forming cells had multipotentiality for chondrogenesis, adipogenesis, calcification, and surface epitopes similar to MSCs. The number was colonies was significantly higher in fibrous Synovium than in adipose Synovium ( P Conclusions We developed a suspended Synovium culture model. Suspended Synovium was able to release MSCs into a noncontacted culture dish through medium in a bottle. Fibrous Synovium was found to release greater numbers of MSCs than adipose Synovium in our culture model. Clinical Relevance This model could be a valuable tool to screen drugs capable of releasing MSCs from the Synovium into synovial fluid.
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comparison of mesenchymal tissues derived stem cells for in vivo chondrogenesis suitable conditions for cell therapy of cartilage defects in rabbit
Cell and Tissue Research, 2008Co-Authors: Hideyuki Koga, Tomoyuki Mochizuki, Takeshi Muneta, Akimoto Nimura, Tsuyoshi Nagase, Youngjin Ju, Ichiro SekiyaAbstract:We previously compared mesenchymal stem cells (MSCs) from a variety of mesenchymal tissues and demonstrated that Synovium-MSCs had the best expansion and chondrogenic ability in vitro in humans and rats. In this study, we compared the in vivo chondrogenic potential of rabbit MSCs. We also examined other parameters to clarify suitable conditions for in vitro and in vivo cartilage formation. MSCs were isolated from bone marrow, Synovium, adipose tissue, and muscle of adult rabbits. Proliferation potential and in vitro chondrogenic potential were compared. Toxicity of the tracer DiI for in vitro chondrogenesis was also examined. MSCs from each tissue were embedded in collagen gel and transplanted into full thickness cartilage defects of rabbits. Cartilage matrix production was compared histologically. The effects of cell density and periosteal patch on the in vivo chondrogenic potential of Synovium-MSCs were also examined. Synovium- and muscle-MSCs had a higher proliferation potential than other cells. Pellets from Synovium- and bone-marrow-MSCs showed abundant cartilage matrix. DiI had no significant influence on in vitro cartilage formation. After transplantation into cartilage defects, Synovium- and bone-marrow-MSCs produced much more cartilage matrix than other cells. When Synovium-MSCs were transplanted at a higher cell density and with a periosteal patch, more abundant cartilage matrix was observed. Thus, Synovium- and bone-marrow-MSCs had greater in vivo chondrogenic potential than adipose- and muscle-MSCs, but Synovium-MSCs had the advantage of a greater proliferation potential. Higher cell density and a periosteum patch were needed to obtain a high production of cartilage matrix by Synovium-MSCs.
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comparison of rat mesenchymal stem cells derived from bone marrow Synovium periosteum adipose tissue and muscle
Cell and Tissue Research, 2007Co-Authors: Hideya Yoshimura, Takeshi Muneta, Akimoto Nimura, Akiko Yokoyama, Hideyuki Koga, Ichiro SekiyaAbstract:Mesenchymal stem cells (MSCs) are increasingly being reported as occurring in a variety of tissues. Although MSCs from human bone marrow are relatively easy to harvest, the isolation of rodent MSCs is more difficult, thereby limiting the number of experiments in vivo. To determine a suitable cell source, we isolated rat MSCs from bone marrow, Synovium, periosteum, adipose, and muscle and compared their properties for yield, expansion, and multipotentiality. After two passages, the cells in each population were CD11b (−), CD45 (−), and CD90 (+). The colony number per nucleated cells derived from Synovium was 100-fold higher than that for cells derived from bone marrow. With regard to expansion potential, Synovium-derived cells were the highest in colony-forming efficiency, fold increase, and growth kinetics. An in vitro chondrogenesis assay demonstrated that the pellets derived from Synovium were heavier, because of their greater production of cartilage matrix, than those from other tissues, indicating their superiority in chondrogenesis. Synovium-derived cells retained their chondrogenic potential after a few passages. The Oil Red-O positive colony-rate assay demonstrated higher adipogenic potential in Synovium- and adipose-derived cells. Alkaline phosphatase activity was greater in periosteum- and muscle-derived cells during calcification. The yield and proliferation potential of rat MSCs from solid tissues was much better than those from bone marrow. In particular, Synovium-derived cells had the greatest potential for both proliferation and chondrogenesis, indicating their usefulness for cartilage study in a rat model.
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higher chondrogenic potential of fibrous Synovium and adipose Synovium derived cells compared with subcutaneous fat derived cells distinguishing properties of mesenchymal stem cells in humans
Arthritis & Rheumatism, 2006Co-Authors: Tomoyuki Mochizuki, Takeshi Muneta, Yusuke Sakaguchi, Akimoto Nimura, Akiko Yokoyama, Hideyuki Koga, Ichiro SekiyaAbstract:Objective Mesenchymal stem cells from Synovium have a greater proliferation and chondrogenic potential than do those from bone marrow, periosteum, fat, and muscle. This study was undertaken to compare fibrous Synovium and adipose Synovium (components of the Synovium with subSynovium) to determine which is a more suitable source for mesenchymal stem cells, especially for cartilage regeneration, and to examine the features of adipose Synovium–derived cells, fibrous Synovium–derived cells, and subcutaneous fat–derived cells to determine their similarities. Methods Human fibrous Synovium, adipose Synovium, and subcutaneous fat were harvested from 4 young donors and 4 elderly donors. After digestion, the nucleated cells were plated at a density considered proper to expand at a maximum rate without colony-to-colony contact. The surface epitopes, proliferative capacity, cloning efficiency, and chondrogenic, osteogenic, and adipogenic differentiation potentials of the cells were compared. Results Fibrous Synovium– and adipose Synovium–derived cells were higher in STRO-1 and CD106 and lower in CD10 compared with subcutaneous fat–derived cells. Cells derived from fibrous and adipose Synovium had higher proliferative potential and colony-forming efficiency compared with subcutaneous fat–derived cells, both in mixed-population and in single-cell–derived cultures. In chondrogenic assays, pellets from fibrous Synovium– and adipose Synovium–derived cells produced more cartilage matrix than did cell pellets from subcutaneous fat. Osteogenic ability was also higher in fibrous Synovium– and adipose Synovium–derived cells, whereas adipogenic potential was nearly indistinguishable among the 3 populations. Differentiation potential of the cells was similar between young and elderly donors. Conclusion Cells derived from the fibrous Synovium and from the adipose Synovium demonstrate comparable chondrogenic potential. Adipose Synovium–derived cells are more similar to fibrous Synovium–derived cells than to subcutaneous fat–derived cells.
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Higher chondrogenic potential of fibrous Synovium– and adipose Synovium–derived cells compared with subcutaneous fat–derived cells: Distinguishing properties of mesenchymal stem cells in humans
Arthritis & Rheumatism, 2006Co-Authors: Tomoyuki Mochizuki, Takeshi Muneta, Yusuke Sakaguchi, Akimoto Nimura, Akiko Yokoyama, Hideyuki Koga, Ichiro SekiyaAbstract:Objective Mesenchymal stem cells from Synovium have a greater proliferation and chondrogenic potential than do those from bone marrow, periosteum, fat, and muscle. This study was undertaken to compare fibrous Synovium and adipose Synovium (components of the Synovium with subSynovium) to determine which is a more suitable source for mesenchymal stem cells, especially for cartilage regeneration, and to examine the features of adipose Synovium–derived cells, fibrous Synovium–derived cells, and subcutaneous fat–derived cells to determine their similarities. Methods Human fibrous Synovium, adipose Synovium, and subcutaneous fat were harvested from 4 young donors and 4 elderly donors. After digestion, the nucleated cells were plated at a density considered proper to expand at a maximum rate without colony-to-colony contact. The surface epitopes, proliferative capacity, cloning efficiency, and chondrogenic, osteogenic, and adipogenic differentiation potentials of the cells were compared. Results Fibrous Synovium– and adipose Synovium–derived cells were higher in STRO-1 and CD106 and lower in CD10 compared with subcutaneous fat–derived cells. Cells derived from fibrous and adipose Synovium had higher proliferative potential and colony-forming efficiency compared with subcutaneous fat–derived cells, both in mixed-population and in single-cell–derived cultures. In chondrogenic assays, pellets from fibrous Synovium– and adipose Synovium–derived cells produced more cartilage matrix than did cell pellets from subcutaneous fat. Osteogenic ability was also higher in fibrous Synovium– and adipose Synovium–derived cells, whereas adipogenic potential was nearly indistinguishable among the 3 populations. Differentiation potential of the cells was similar between young and elderly donors. Conclusion Cells derived from the fibrous Synovium and from the adipose Synovium demonstrate comparable chondrogenic potential. Adipose Synovium–derived cells are more similar to fibrous Synovium–derived cells than to subcutaneous fat–derived cells.
Takeshi Muneta - One of the best experts on this subject based on the ideXlab platform.
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Fibrous Synovium Releases Higher Numbers of Mesenchymal Stem Cells Than Adipose Synovium in a Suspended Synovium Culture Model.
Arthroscopy, 2016Co-Authors: Kenta Katagiri, Takeshi Muneta, Yu Matsukura, Nobutake Ozeki, Mitsuru Mizuno, Hisako Katano, Ichiro SekiyaAbstract:Purpose To develop an in vitro model, the "suspended Synovium culture model," to demonstrate the mobilization of mesenchymal stem cells (MSCs) from the Synovium into a noncontacted culture dish through culture medium. In addition, to examine which Synovium, fibrous Synovium or adipose Synovium, released more MSCs in the knee with osteoarthritis. Methods Human synovial tissue was harvested during total knee arthroplasty from knee joints of 34 patients with osteoarthritis (28 patients: only fibrous Synovium, 6 patients: fibrous and adipose Synovium). One gram of Synovium was suspended with a thread in a bottle containing 40 mL of culture medium and a 3.5-cm-diameter culture dish at the bottom. After 7 days, the culture dish in the bottle was examined. For the cells harvested, multipotentiality and surface epitopes were analyzed. The numbers of colonies derived from fibrous Synovium and adipose Synovium were also compared. Results Colonies of spindle-shaped cells were observed in the culture dish in all 28 donors. Colonies numbered 26 on average, and the cells derived from colony-forming cells had multipotentiality for chondrogenesis, adipogenesis, calcification, and surface epitopes similar to MSCs. The number was colonies was significantly higher in fibrous Synovium than in adipose Synovium ( P Conclusions We developed a suspended Synovium culture model. Suspended Synovium was able to release MSCs into a noncontacted culture dish through medium in a bottle. Fibrous Synovium was found to release greater numbers of MSCs than adipose Synovium in our culture model. Clinical Relevance This model could be a valuable tool to screen drugs capable of releasing MSCs from the Synovium into synovial fluid.
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comparison of mesenchymal tissues derived stem cells for in vivo chondrogenesis suitable conditions for cell therapy of cartilage defects in rabbit
Cell and Tissue Research, 2008Co-Authors: Hideyuki Koga, Tomoyuki Mochizuki, Takeshi Muneta, Akimoto Nimura, Tsuyoshi Nagase, Youngjin Ju, Ichiro SekiyaAbstract:We previously compared mesenchymal stem cells (MSCs) from a variety of mesenchymal tissues and demonstrated that Synovium-MSCs had the best expansion and chondrogenic ability in vitro in humans and rats. In this study, we compared the in vivo chondrogenic potential of rabbit MSCs. We also examined other parameters to clarify suitable conditions for in vitro and in vivo cartilage formation. MSCs were isolated from bone marrow, Synovium, adipose tissue, and muscle of adult rabbits. Proliferation potential and in vitro chondrogenic potential were compared. Toxicity of the tracer DiI for in vitro chondrogenesis was also examined. MSCs from each tissue were embedded in collagen gel and transplanted into full thickness cartilage defects of rabbits. Cartilage matrix production was compared histologically. The effects of cell density and periosteal patch on the in vivo chondrogenic potential of Synovium-MSCs were also examined. Synovium- and muscle-MSCs had a higher proliferation potential than other cells. Pellets from Synovium- and bone-marrow-MSCs showed abundant cartilage matrix. DiI had no significant influence on in vitro cartilage formation. After transplantation into cartilage defects, Synovium- and bone-marrow-MSCs produced much more cartilage matrix than other cells. When Synovium-MSCs were transplanted at a higher cell density and with a periosteal patch, more abundant cartilage matrix was observed. Thus, Synovium- and bone-marrow-MSCs had greater in vivo chondrogenic potential than adipose- and muscle-MSCs, but Synovium-MSCs had the advantage of a greater proliferation potential. Higher cell density and a periosteum patch were needed to obtain a high production of cartilage matrix by Synovium-MSCs.
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comparison of rat mesenchymal stem cells derived from bone marrow Synovium periosteum adipose tissue and muscle
Cell and Tissue Research, 2007Co-Authors: Hideya Yoshimura, Takeshi Muneta, Akimoto Nimura, Akiko Yokoyama, Hideyuki Koga, Ichiro SekiyaAbstract:Mesenchymal stem cells (MSCs) are increasingly being reported as occurring in a variety of tissues. Although MSCs from human bone marrow are relatively easy to harvest, the isolation of rodent MSCs is more difficult, thereby limiting the number of experiments in vivo. To determine a suitable cell source, we isolated rat MSCs from bone marrow, Synovium, periosteum, adipose, and muscle and compared their properties for yield, expansion, and multipotentiality. After two passages, the cells in each population were CD11b (−), CD45 (−), and CD90 (+). The colony number per nucleated cells derived from Synovium was 100-fold higher than that for cells derived from bone marrow. With regard to expansion potential, Synovium-derived cells were the highest in colony-forming efficiency, fold increase, and growth kinetics. An in vitro chondrogenesis assay demonstrated that the pellets derived from Synovium were heavier, because of their greater production of cartilage matrix, than those from other tissues, indicating their superiority in chondrogenesis. Synovium-derived cells retained their chondrogenic potential after a few passages. The Oil Red-O positive colony-rate assay demonstrated higher adipogenic potential in Synovium- and adipose-derived cells. Alkaline phosphatase activity was greater in periosteum- and muscle-derived cells during calcification. The yield and proliferation potential of rat MSCs from solid tissues was much better than those from bone marrow. In particular, Synovium-derived cells had the greatest potential for both proliferation and chondrogenesis, indicating their usefulness for cartilage study in a rat model.
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higher chondrogenic potential of fibrous Synovium and adipose Synovium derived cells compared with subcutaneous fat derived cells distinguishing properties of mesenchymal stem cells in humans
Arthritis & Rheumatism, 2006Co-Authors: Tomoyuki Mochizuki, Takeshi Muneta, Yusuke Sakaguchi, Akimoto Nimura, Akiko Yokoyama, Hideyuki Koga, Ichiro SekiyaAbstract:Objective Mesenchymal stem cells from Synovium have a greater proliferation and chondrogenic potential than do those from bone marrow, periosteum, fat, and muscle. This study was undertaken to compare fibrous Synovium and adipose Synovium (components of the Synovium with subSynovium) to determine which is a more suitable source for mesenchymal stem cells, especially for cartilage regeneration, and to examine the features of adipose Synovium–derived cells, fibrous Synovium–derived cells, and subcutaneous fat–derived cells to determine their similarities. Methods Human fibrous Synovium, adipose Synovium, and subcutaneous fat were harvested from 4 young donors and 4 elderly donors. After digestion, the nucleated cells were plated at a density considered proper to expand at a maximum rate without colony-to-colony contact. The surface epitopes, proliferative capacity, cloning efficiency, and chondrogenic, osteogenic, and adipogenic differentiation potentials of the cells were compared. Results Fibrous Synovium– and adipose Synovium–derived cells were higher in STRO-1 and CD106 and lower in CD10 compared with subcutaneous fat–derived cells. Cells derived from fibrous and adipose Synovium had higher proliferative potential and colony-forming efficiency compared with subcutaneous fat–derived cells, both in mixed-population and in single-cell–derived cultures. In chondrogenic assays, pellets from fibrous Synovium– and adipose Synovium–derived cells produced more cartilage matrix than did cell pellets from subcutaneous fat. Osteogenic ability was also higher in fibrous Synovium– and adipose Synovium–derived cells, whereas adipogenic potential was nearly indistinguishable among the 3 populations. Differentiation potential of the cells was similar between young and elderly donors. Conclusion Cells derived from the fibrous Synovium and from the adipose Synovium demonstrate comparable chondrogenic potential. Adipose Synovium–derived cells are more similar to fibrous Synovium–derived cells than to subcutaneous fat–derived cells.
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Higher chondrogenic potential of fibrous Synovium– and adipose Synovium–derived cells compared with subcutaneous fat–derived cells: Distinguishing properties of mesenchymal stem cells in humans
Arthritis & Rheumatism, 2006Co-Authors: Tomoyuki Mochizuki, Takeshi Muneta, Yusuke Sakaguchi, Akimoto Nimura, Akiko Yokoyama, Hideyuki Koga, Ichiro SekiyaAbstract:Objective Mesenchymal stem cells from Synovium have a greater proliferation and chondrogenic potential than do those from bone marrow, periosteum, fat, and muscle. This study was undertaken to compare fibrous Synovium and adipose Synovium (components of the Synovium with subSynovium) to determine which is a more suitable source for mesenchymal stem cells, especially for cartilage regeneration, and to examine the features of adipose Synovium–derived cells, fibrous Synovium–derived cells, and subcutaneous fat–derived cells to determine their similarities. Methods Human fibrous Synovium, adipose Synovium, and subcutaneous fat were harvested from 4 young donors and 4 elderly donors. After digestion, the nucleated cells were plated at a density considered proper to expand at a maximum rate without colony-to-colony contact. The surface epitopes, proliferative capacity, cloning efficiency, and chondrogenic, osteogenic, and adipogenic differentiation potentials of the cells were compared. Results Fibrous Synovium– and adipose Synovium–derived cells were higher in STRO-1 and CD106 and lower in CD10 compared with subcutaneous fat–derived cells. Cells derived from fibrous and adipose Synovium had higher proliferative potential and colony-forming efficiency compared with subcutaneous fat–derived cells, both in mixed-population and in single-cell–derived cultures. In chondrogenic assays, pellets from fibrous Synovium– and adipose Synovium–derived cells produced more cartilage matrix than did cell pellets from subcutaneous fat. Osteogenic ability was also higher in fibrous Synovium– and adipose Synovium–derived cells, whereas adipogenic potential was nearly indistinguishable among the 3 populations. Differentiation potential of the cells was similar between young and elderly donors. Conclusion Cells derived from the fibrous Synovium and from the adipose Synovium demonstrate comparable chondrogenic potential. Adipose Synovium–derived cells are more similar to fibrous Synovium–derived cells than to subcutaneous fat–derived cells.
Yusuke Sakaguchi - One of the best experts on this subject based on the ideXlab platform.
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higher chondrogenic potential of fibrous Synovium and adipose Synovium derived cells compared with subcutaneous fat derived cells distinguishing properties of mesenchymal stem cells in humans
Arthritis & Rheumatism, 2006Co-Authors: Tomoyuki Mochizuki, Takeshi Muneta, Yusuke Sakaguchi, Akimoto Nimura, Akiko Yokoyama, Hideyuki Koga, Ichiro SekiyaAbstract:Objective Mesenchymal stem cells from Synovium have a greater proliferation and chondrogenic potential than do those from bone marrow, periosteum, fat, and muscle. This study was undertaken to compare fibrous Synovium and adipose Synovium (components of the Synovium with subSynovium) to determine which is a more suitable source for mesenchymal stem cells, especially for cartilage regeneration, and to examine the features of adipose Synovium–derived cells, fibrous Synovium–derived cells, and subcutaneous fat–derived cells to determine their similarities. Methods Human fibrous Synovium, adipose Synovium, and subcutaneous fat were harvested from 4 young donors and 4 elderly donors. After digestion, the nucleated cells were plated at a density considered proper to expand at a maximum rate without colony-to-colony contact. The surface epitopes, proliferative capacity, cloning efficiency, and chondrogenic, osteogenic, and adipogenic differentiation potentials of the cells were compared. Results Fibrous Synovium– and adipose Synovium–derived cells were higher in STRO-1 and CD106 and lower in CD10 compared with subcutaneous fat–derived cells. Cells derived from fibrous and adipose Synovium had higher proliferative potential and colony-forming efficiency compared with subcutaneous fat–derived cells, both in mixed-population and in single-cell–derived cultures. In chondrogenic assays, pellets from fibrous Synovium– and adipose Synovium–derived cells produced more cartilage matrix than did cell pellets from subcutaneous fat. Osteogenic ability was also higher in fibrous Synovium– and adipose Synovium–derived cells, whereas adipogenic potential was nearly indistinguishable among the 3 populations. Differentiation potential of the cells was similar between young and elderly donors. Conclusion Cells derived from the fibrous Synovium and from the adipose Synovium demonstrate comparable chondrogenic potential. Adipose Synovium–derived cells are more similar to fibrous Synovium–derived cells than to subcutaneous fat–derived cells.
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Higher chondrogenic potential of fibrous Synovium– and adipose Synovium–derived cells compared with subcutaneous fat–derived cells: Distinguishing properties of mesenchymal stem cells in humans
Arthritis & Rheumatism, 2006Co-Authors: Tomoyuki Mochizuki, Takeshi Muneta, Yusuke Sakaguchi, Akimoto Nimura, Akiko Yokoyama, Hideyuki Koga, Ichiro SekiyaAbstract:Objective Mesenchymal stem cells from Synovium have a greater proliferation and chondrogenic potential than do those from bone marrow, periosteum, fat, and muscle. This study was undertaken to compare fibrous Synovium and adipose Synovium (components of the Synovium with subSynovium) to determine which is a more suitable source for mesenchymal stem cells, especially for cartilage regeneration, and to examine the features of adipose Synovium–derived cells, fibrous Synovium–derived cells, and subcutaneous fat–derived cells to determine their similarities. Methods Human fibrous Synovium, adipose Synovium, and subcutaneous fat were harvested from 4 young donors and 4 elderly donors. After digestion, the nucleated cells were plated at a density considered proper to expand at a maximum rate without colony-to-colony contact. The surface epitopes, proliferative capacity, cloning efficiency, and chondrogenic, osteogenic, and adipogenic differentiation potentials of the cells were compared. Results Fibrous Synovium– and adipose Synovium–derived cells were higher in STRO-1 and CD106 and lower in CD10 compared with subcutaneous fat–derived cells. Cells derived from fibrous and adipose Synovium had higher proliferative potential and colony-forming efficiency compared with subcutaneous fat–derived cells, both in mixed-population and in single-cell–derived cultures. In chondrogenic assays, pellets from fibrous Synovium– and adipose Synovium–derived cells produced more cartilage matrix than did cell pellets from subcutaneous fat. Osteogenic ability was also higher in fibrous Synovium– and adipose Synovium–derived cells, whereas adipogenic potential was nearly indistinguishable among the 3 populations. Differentiation potential of the cells was similar between young and elderly donors. Conclusion Cells derived from the fibrous Synovium and from the adipose Synovium demonstrate comparable chondrogenic potential. Adipose Synovium–derived cells are more similar to fibrous Synovium–derived cells than to subcutaneous fat–derived cells.
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in vitro chondrogenesis of human Synovium derived mesenchymal stem cells optimal condition and comparison with bone marrow derived cells
Journal of Cellular Biochemistry, 2006Co-Authors: Shinichi Shirasawa, Yusuke Sakaguchi, Ichiro Sekiya, Kazuyoshi Yagishita, Shizuko Ichinose, Takeshi MunetaAbstract:There are increasing reports that mesenchymal stem cells (MSCs) are present in various tissues other than bone marrow, including Synovium. Here we investigated the optimal conditions for in vitro chondrogenesis of human Synovium-derived MSCs and compared these cells with bone marrow-derived MSCs, especially in terms of their chondrogenesis potential. Synovium and bone marrow were harvested from six donors during knee operations for ligament injuries. Digested Synovium cells or nucleated cells from bone marrow were expanded clonally. A pellet culture system was used for chondrogenesis, and the best combination of up to three cytokines of the seven assessed. Synovium-derived MSCs plated at a lower density expanded more rapidly. Contrary to previous reports, a combination of TGFβ and dexamethasone was not sufficient to induce chondrogenesis. However, addition of BMP2 to TGFβ and dexamethasone dramatically increased cartilage pellet size and the synthesis of cartilage matrix. The cartilage pellets were also analyzed by electron microscopy and immunohistology. DNA content per pellet decreased during chondrogenesis, indicating the pellet increased its size through the accumulation of newly synthesized extracellular matrix. Sequential chondrogenic gene expression was demonstrated by RT-PCR. Synovium-derived MSCs looked similar to the bone marrow-derived MSCs in their surface epitopes and proliferation potential; however, cartilage pellets from Synovium were significantly larger than those from bone marrow in patient-matched comparisons. We demonstrated that the combination of TGFβ, dexamethasone, and BMP2 was optimal for in vitro chondrogenesis of Synovium-derived MSCs and that the Synovium-derived MSCs have a greater chondrogenesis potential than bone marrow-derived MSCs. © 2005 Wiley-Liss, Inc.
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comparison of human stem cells derived from various mesenchymal tissues superiority of Synovium as a cell source
Arthritis & Rheumatism, 2005Co-Authors: Yusuke Sakaguchi, Ichiro Sekiya, Kazuyoshi Yagishita, Takeshi MunetaAbstract:OBJECTIVE: To compare the properties of human mesenchymal stem cells (MSCs) isolated from bone marrow, Synovium, periosteum, skeletal muscle, and adipose tissue. METHODS: Human mesenchymal tissues were obtained from 8 donors during knee surgery for ligament injury. After collagenase digestion or gradient-density separation, nucleated cells were plated at an appropriate density for expansion at the maximum rate without colony-to-colony contact. Yield, expandability, differentiation potential, and epitope profile were compared among MSCs from the 5 different tissue sources. RESULTS: Colony number per 10(3) nucleated cells was lower, and cell number per colony was higher, in bone marrow than in other mesenchymal tissues. When the cells were replated at low density every 14 days, bone marrow-, Synovium-, and periosteum-derived cells retained their proliferation ability even at passage 10. In chondrogenesis studies in which the cells were pelleted and cultured in vitro, pellets from bone marrow-, Synovium-, and periosteum-derived cells were shown to be larger and stained more extensively for cartilage matrix. Synovium-derived cells, in particular, had the greatest ability for chondrogenesis. In adipogenesis experiments, the frequency of oil red O-positive colonies was highest in Synovium- and adipose tissue-derived cells. In studies of osteogenesis, the rate of alizarin red-positive colonies was highest in bone marrow-, Synovium-, and periosteum-derived cells. For epitope profiling, 15 surface antigens were measured. Most appeared to have similar epitope profiles irrespective of cell source. CONCLUSION: Our findings indicate that there are significant differences in MSC properties according to tissue source, beyond donor and experimental variation. Superiority of Synovium as a potential source of MSCs for clinical applications was demonstrated.
Hideyuki Koga - One of the best experts on this subject based on the ideXlab platform.
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comparison of mesenchymal tissues derived stem cells for in vivo chondrogenesis suitable conditions for cell therapy of cartilage defects in rabbit
Cell and Tissue Research, 2008Co-Authors: Hideyuki Koga, Tomoyuki Mochizuki, Takeshi Muneta, Akimoto Nimura, Tsuyoshi Nagase, Youngjin Ju, Ichiro SekiyaAbstract:We previously compared mesenchymal stem cells (MSCs) from a variety of mesenchymal tissues and demonstrated that Synovium-MSCs had the best expansion and chondrogenic ability in vitro in humans and rats. In this study, we compared the in vivo chondrogenic potential of rabbit MSCs. We also examined other parameters to clarify suitable conditions for in vitro and in vivo cartilage formation. MSCs were isolated from bone marrow, Synovium, adipose tissue, and muscle of adult rabbits. Proliferation potential and in vitro chondrogenic potential were compared. Toxicity of the tracer DiI for in vitro chondrogenesis was also examined. MSCs from each tissue were embedded in collagen gel and transplanted into full thickness cartilage defects of rabbits. Cartilage matrix production was compared histologically. The effects of cell density and periosteal patch on the in vivo chondrogenic potential of Synovium-MSCs were also examined. Synovium- and muscle-MSCs had a higher proliferation potential than other cells. Pellets from Synovium- and bone-marrow-MSCs showed abundant cartilage matrix. DiI had no significant influence on in vitro cartilage formation. After transplantation into cartilage defects, Synovium- and bone-marrow-MSCs produced much more cartilage matrix than other cells. When Synovium-MSCs were transplanted at a higher cell density and with a periosteal patch, more abundant cartilage matrix was observed. Thus, Synovium- and bone-marrow-MSCs had greater in vivo chondrogenic potential than adipose- and muscle-MSCs, but Synovium-MSCs had the advantage of a greater proliferation potential. Higher cell density and a periosteum patch were needed to obtain a high production of cartilage matrix by Synovium-MSCs.
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comparison of rat mesenchymal stem cells derived from bone marrow Synovium periosteum adipose tissue and muscle
Cell and Tissue Research, 2007Co-Authors: Hideya Yoshimura, Takeshi Muneta, Akimoto Nimura, Akiko Yokoyama, Hideyuki Koga, Ichiro SekiyaAbstract:Mesenchymal stem cells (MSCs) are increasingly being reported as occurring in a variety of tissues. Although MSCs from human bone marrow are relatively easy to harvest, the isolation of rodent MSCs is more difficult, thereby limiting the number of experiments in vivo. To determine a suitable cell source, we isolated rat MSCs from bone marrow, Synovium, periosteum, adipose, and muscle and compared their properties for yield, expansion, and multipotentiality. After two passages, the cells in each population were CD11b (−), CD45 (−), and CD90 (+). The colony number per nucleated cells derived from Synovium was 100-fold higher than that for cells derived from bone marrow. With regard to expansion potential, Synovium-derived cells were the highest in colony-forming efficiency, fold increase, and growth kinetics. An in vitro chondrogenesis assay demonstrated that the pellets derived from Synovium were heavier, because of their greater production of cartilage matrix, than those from other tissues, indicating their superiority in chondrogenesis. Synovium-derived cells retained their chondrogenic potential after a few passages. The Oil Red-O positive colony-rate assay demonstrated higher adipogenic potential in Synovium- and adipose-derived cells. Alkaline phosphatase activity was greater in periosteum- and muscle-derived cells during calcification. The yield and proliferation potential of rat MSCs from solid tissues was much better than those from bone marrow. In particular, Synovium-derived cells had the greatest potential for both proliferation and chondrogenesis, indicating their usefulness for cartilage study in a rat model.
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higher chondrogenic potential of fibrous Synovium and adipose Synovium derived cells compared with subcutaneous fat derived cells distinguishing properties of mesenchymal stem cells in humans
Arthritis & Rheumatism, 2006Co-Authors: Tomoyuki Mochizuki, Takeshi Muneta, Yusuke Sakaguchi, Akimoto Nimura, Akiko Yokoyama, Hideyuki Koga, Ichiro SekiyaAbstract:Objective Mesenchymal stem cells from Synovium have a greater proliferation and chondrogenic potential than do those from bone marrow, periosteum, fat, and muscle. This study was undertaken to compare fibrous Synovium and adipose Synovium (components of the Synovium with subSynovium) to determine which is a more suitable source for mesenchymal stem cells, especially for cartilage regeneration, and to examine the features of adipose Synovium–derived cells, fibrous Synovium–derived cells, and subcutaneous fat–derived cells to determine their similarities. Methods Human fibrous Synovium, adipose Synovium, and subcutaneous fat were harvested from 4 young donors and 4 elderly donors. After digestion, the nucleated cells were plated at a density considered proper to expand at a maximum rate without colony-to-colony contact. The surface epitopes, proliferative capacity, cloning efficiency, and chondrogenic, osteogenic, and adipogenic differentiation potentials of the cells were compared. Results Fibrous Synovium– and adipose Synovium–derived cells were higher in STRO-1 and CD106 and lower in CD10 compared with subcutaneous fat–derived cells. Cells derived from fibrous and adipose Synovium had higher proliferative potential and colony-forming efficiency compared with subcutaneous fat–derived cells, both in mixed-population and in single-cell–derived cultures. In chondrogenic assays, pellets from fibrous Synovium– and adipose Synovium–derived cells produced more cartilage matrix than did cell pellets from subcutaneous fat. Osteogenic ability was also higher in fibrous Synovium– and adipose Synovium–derived cells, whereas adipogenic potential was nearly indistinguishable among the 3 populations. Differentiation potential of the cells was similar between young and elderly donors. Conclusion Cells derived from the fibrous Synovium and from the adipose Synovium demonstrate comparable chondrogenic potential. Adipose Synovium–derived cells are more similar to fibrous Synovium–derived cells than to subcutaneous fat–derived cells.
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Higher chondrogenic potential of fibrous Synovium– and adipose Synovium–derived cells compared with subcutaneous fat–derived cells: Distinguishing properties of mesenchymal stem cells in humans
Arthritis & Rheumatism, 2006Co-Authors: Tomoyuki Mochizuki, Takeshi Muneta, Yusuke Sakaguchi, Akimoto Nimura, Akiko Yokoyama, Hideyuki Koga, Ichiro SekiyaAbstract:Objective Mesenchymal stem cells from Synovium have a greater proliferation and chondrogenic potential than do those from bone marrow, periosteum, fat, and muscle. This study was undertaken to compare fibrous Synovium and adipose Synovium (components of the Synovium with subSynovium) to determine which is a more suitable source for mesenchymal stem cells, especially for cartilage regeneration, and to examine the features of adipose Synovium–derived cells, fibrous Synovium–derived cells, and subcutaneous fat–derived cells to determine their similarities. Methods Human fibrous Synovium, adipose Synovium, and subcutaneous fat were harvested from 4 young donors and 4 elderly donors. After digestion, the nucleated cells were plated at a density considered proper to expand at a maximum rate without colony-to-colony contact. The surface epitopes, proliferative capacity, cloning efficiency, and chondrogenic, osteogenic, and adipogenic differentiation potentials of the cells were compared. Results Fibrous Synovium– and adipose Synovium–derived cells were higher in STRO-1 and CD106 and lower in CD10 compared with subcutaneous fat–derived cells. Cells derived from fibrous and adipose Synovium had higher proliferative potential and colony-forming efficiency compared with subcutaneous fat–derived cells, both in mixed-population and in single-cell–derived cultures. In chondrogenic assays, pellets from fibrous Synovium– and adipose Synovium–derived cells produced more cartilage matrix than did cell pellets from subcutaneous fat. Osteogenic ability was also higher in fibrous Synovium– and adipose Synovium–derived cells, whereas adipogenic potential was nearly indistinguishable among the 3 populations. Differentiation potential of the cells was similar between young and elderly donors. Conclusion Cells derived from the fibrous Synovium and from the adipose Synovium demonstrate comparable chondrogenic potential. Adipose Synovium–derived cells are more similar to fibrous Synovium–derived cells than to subcutaneous fat–derived cells.
Akimoto Nimura - One of the best experts on this subject based on the ideXlab platform.
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comparison of mesenchymal tissues derived stem cells for in vivo chondrogenesis suitable conditions for cell therapy of cartilage defects in rabbit
Cell and Tissue Research, 2008Co-Authors: Hideyuki Koga, Tomoyuki Mochizuki, Takeshi Muneta, Akimoto Nimura, Tsuyoshi Nagase, Youngjin Ju, Ichiro SekiyaAbstract:We previously compared mesenchymal stem cells (MSCs) from a variety of mesenchymal tissues and demonstrated that Synovium-MSCs had the best expansion and chondrogenic ability in vitro in humans and rats. In this study, we compared the in vivo chondrogenic potential of rabbit MSCs. We also examined other parameters to clarify suitable conditions for in vitro and in vivo cartilage formation. MSCs were isolated from bone marrow, Synovium, adipose tissue, and muscle of adult rabbits. Proliferation potential and in vitro chondrogenic potential were compared. Toxicity of the tracer DiI for in vitro chondrogenesis was also examined. MSCs from each tissue were embedded in collagen gel and transplanted into full thickness cartilage defects of rabbits. Cartilage matrix production was compared histologically. The effects of cell density and periosteal patch on the in vivo chondrogenic potential of Synovium-MSCs were also examined. Synovium- and muscle-MSCs had a higher proliferation potential than other cells. Pellets from Synovium- and bone-marrow-MSCs showed abundant cartilage matrix. DiI had no significant influence on in vitro cartilage formation. After transplantation into cartilage defects, Synovium- and bone-marrow-MSCs produced much more cartilage matrix than other cells. When Synovium-MSCs were transplanted at a higher cell density and with a periosteal patch, more abundant cartilage matrix was observed. Thus, Synovium- and bone-marrow-MSCs had greater in vivo chondrogenic potential than adipose- and muscle-MSCs, but Synovium-MSCs had the advantage of a greater proliferation potential. Higher cell density and a periosteum patch were needed to obtain a high production of cartilage matrix by Synovium-MSCs.
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comparison of rat mesenchymal stem cells derived from bone marrow Synovium periosteum adipose tissue and muscle
Cell and Tissue Research, 2007Co-Authors: Hideya Yoshimura, Takeshi Muneta, Akimoto Nimura, Akiko Yokoyama, Hideyuki Koga, Ichiro SekiyaAbstract:Mesenchymal stem cells (MSCs) are increasingly being reported as occurring in a variety of tissues. Although MSCs from human bone marrow are relatively easy to harvest, the isolation of rodent MSCs is more difficult, thereby limiting the number of experiments in vivo. To determine a suitable cell source, we isolated rat MSCs from bone marrow, Synovium, periosteum, adipose, and muscle and compared their properties for yield, expansion, and multipotentiality. After two passages, the cells in each population were CD11b (−), CD45 (−), and CD90 (+). The colony number per nucleated cells derived from Synovium was 100-fold higher than that for cells derived from bone marrow. With regard to expansion potential, Synovium-derived cells were the highest in colony-forming efficiency, fold increase, and growth kinetics. An in vitro chondrogenesis assay demonstrated that the pellets derived from Synovium were heavier, because of their greater production of cartilage matrix, than those from other tissues, indicating their superiority in chondrogenesis. Synovium-derived cells retained their chondrogenic potential after a few passages. The Oil Red-O positive colony-rate assay demonstrated higher adipogenic potential in Synovium- and adipose-derived cells. Alkaline phosphatase activity was greater in periosteum- and muscle-derived cells during calcification. The yield and proliferation potential of rat MSCs from solid tissues was much better than those from bone marrow. In particular, Synovium-derived cells had the greatest potential for both proliferation and chondrogenesis, indicating their usefulness for cartilage study in a rat model.
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higher chondrogenic potential of fibrous Synovium and adipose Synovium derived cells compared with subcutaneous fat derived cells distinguishing properties of mesenchymal stem cells in humans
Arthritis & Rheumatism, 2006Co-Authors: Tomoyuki Mochizuki, Takeshi Muneta, Yusuke Sakaguchi, Akimoto Nimura, Akiko Yokoyama, Hideyuki Koga, Ichiro SekiyaAbstract:Objective Mesenchymal stem cells from Synovium have a greater proliferation and chondrogenic potential than do those from bone marrow, periosteum, fat, and muscle. This study was undertaken to compare fibrous Synovium and adipose Synovium (components of the Synovium with subSynovium) to determine which is a more suitable source for mesenchymal stem cells, especially for cartilage regeneration, and to examine the features of adipose Synovium–derived cells, fibrous Synovium–derived cells, and subcutaneous fat–derived cells to determine their similarities. Methods Human fibrous Synovium, adipose Synovium, and subcutaneous fat were harvested from 4 young donors and 4 elderly donors. After digestion, the nucleated cells were plated at a density considered proper to expand at a maximum rate without colony-to-colony contact. The surface epitopes, proliferative capacity, cloning efficiency, and chondrogenic, osteogenic, and adipogenic differentiation potentials of the cells were compared. Results Fibrous Synovium– and adipose Synovium–derived cells were higher in STRO-1 and CD106 and lower in CD10 compared with subcutaneous fat–derived cells. Cells derived from fibrous and adipose Synovium had higher proliferative potential and colony-forming efficiency compared with subcutaneous fat–derived cells, both in mixed-population and in single-cell–derived cultures. In chondrogenic assays, pellets from fibrous Synovium– and adipose Synovium–derived cells produced more cartilage matrix than did cell pellets from subcutaneous fat. Osteogenic ability was also higher in fibrous Synovium– and adipose Synovium–derived cells, whereas adipogenic potential was nearly indistinguishable among the 3 populations. Differentiation potential of the cells was similar between young and elderly donors. Conclusion Cells derived from the fibrous Synovium and from the adipose Synovium demonstrate comparable chondrogenic potential. Adipose Synovium–derived cells are more similar to fibrous Synovium–derived cells than to subcutaneous fat–derived cells.
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Higher chondrogenic potential of fibrous Synovium– and adipose Synovium–derived cells compared with subcutaneous fat–derived cells: Distinguishing properties of mesenchymal stem cells in humans
Arthritis & Rheumatism, 2006Co-Authors: Tomoyuki Mochizuki, Takeshi Muneta, Yusuke Sakaguchi, Akimoto Nimura, Akiko Yokoyama, Hideyuki Koga, Ichiro SekiyaAbstract:Objective Mesenchymal stem cells from Synovium have a greater proliferation and chondrogenic potential than do those from bone marrow, periosteum, fat, and muscle. This study was undertaken to compare fibrous Synovium and adipose Synovium (components of the Synovium with subSynovium) to determine which is a more suitable source for mesenchymal stem cells, especially for cartilage regeneration, and to examine the features of adipose Synovium–derived cells, fibrous Synovium–derived cells, and subcutaneous fat–derived cells to determine their similarities. Methods Human fibrous Synovium, adipose Synovium, and subcutaneous fat were harvested from 4 young donors and 4 elderly donors. After digestion, the nucleated cells were plated at a density considered proper to expand at a maximum rate without colony-to-colony contact. The surface epitopes, proliferative capacity, cloning efficiency, and chondrogenic, osteogenic, and adipogenic differentiation potentials of the cells were compared. Results Fibrous Synovium– and adipose Synovium–derived cells were higher in STRO-1 and CD106 and lower in CD10 compared with subcutaneous fat–derived cells. Cells derived from fibrous and adipose Synovium had higher proliferative potential and colony-forming efficiency compared with subcutaneous fat–derived cells, both in mixed-population and in single-cell–derived cultures. In chondrogenic assays, pellets from fibrous Synovium– and adipose Synovium–derived cells produced more cartilage matrix than did cell pellets from subcutaneous fat. Osteogenic ability was also higher in fibrous Synovium– and adipose Synovium–derived cells, whereas adipogenic potential was nearly indistinguishable among the 3 populations. Differentiation potential of the cells was similar between young and elderly donors. Conclusion Cells derived from the fibrous Synovium and from the adipose Synovium demonstrate comparable chondrogenic potential. Adipose Synovium–derived cells are more similar to fibrous Synovium–derived cells than to subcutaneous fat–derived cells.
