Taenia Pisiformis

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Shaohua Zhang - One of the best experts on this subject based on the ideXlab platform.

  • Characterization of exosome-like vesicles derived from Taenia Pisiformis cysticercus and their immunoregulatory role on macrophages.
    Parasites & Vectors, 2020
    Co-Authors: Liqun Wang, Shaohua Zhang, Panhong Liang, Taoshan Li, Yanping Li
    Abstract:

    BACKGROUND Taenia Pisiformis is one of the most common intestinal parasites in canines, and leads to serious economic losses in the rabbit breeding industry. Exosome-like vesicles from parasites play crucial roles in host-parasite interactions by transferring cargo from parasites to host cells and by modulating host immunological response through inducing production of host-derived cytokines. Nevertheless, the mechanism by which exosome-like vesicles from T. Pisiformis cysticercus regulate the macrophage immune response remains unknown. METHODS Using ultracentrifugation, we isolated exosome-like vesicles from excretory/secretory products (ESP) of T. Pisiformis cysticercus. The morphology and size of purified vesicles were confirmed by transmission electron microscopy (TEM) and nanoparticle tracking analysis (NTA). The components of proteins and miRNAs within these vesicles were identified by proteomic analysis and high-throughput small RNA sequencing. The biological function of targets of exosomal miRNAs was predicted by Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway analysis. Moreover, the expression of Th1- and Th2-type immune response associated cytokines in RAW264.7 macrophages were evaluated by qPCR and ELISA. We found that exosome-like vesicles were typical cup-shaped vesicles with diameters from 30 to 150 nm. A total of 87 proteins were identified by proteomic analysis, including proteins prominently associated with exosome-like vesicles biogenesis and vesicle trafficking. 41 known miRNAs and 18 novel miRNAs were identified in the exosome-like vesicles. Eleven selected miRNAs, including 7 known miRNAs (miR-71-5p, miR-10a-5p, miR-let-7-5p, miR-745-3p, miR-219-5p, miR-124-3p and miR-4989-3p) and 4 novel miRNAs (novel-mir-3, novel-mir-7, novel-mir-8 and novel-mir-11) were validated to exist in metacestiodes and exosome-like vesicles of T. Pisiformis cysticercus by qPCR. The functions of most targets of exosomal miRNAs were mainly associated with signal transduction and the immune system. Additionally, T. Pisiformis cysticercus-derived vesicles induced the production of IL-4, IL-6, IL-10, IL-13 and Arg-1, but downregulated the expression of IL-12, IFN-γ and iNOS in RAW264.7 macrophages. CONCLUSIONS We demonstrated that proteins and miRNAs enclosed within exosome-like vesicles from T. Pisiformis cysticercus have immunomodulatory functions. Furthermore, exosome-like vesicles were shown to induce the macrophage Th2-type immune response in vitro. Our study suggests that exosome-like vesicles play an important role in the interaction between cysticerci and their hosts.

  • characterization of exosome like vesicles derived from Taenia Pisiformis cysticercus and their immunoregulatory role on macrophages
    Parasites & Vectors, 2020
    Co-Authors: Liqun Wang, Shaohua Zhang, Panhong Liang, Taoshan Li, Yanping Li
    Abstract:

    Background Taenia Pisiformis is one of the most common intestinal parasites in canines, and leads to serious economic losses in the rabbit breeding industry. Exosome-like vesicles from parasites play crucial roles in host-parasite interactions by transferring cargo from parasites to host cells and by modulating host immunological response through inducing production of host-derived cytokines. Nevertheless, the mechanism by which exosome-like vesicles from T. Pisiformis cysticercus regulate the macrophage immune response remains unknown. Methods Using ultracentrifugation, we isolated exosome-like vesicles from excretory/secretory products (ESP) of T. Pisiformis cysticercus. The morphology and size of purified vesicles were confirmed by transmission electron microscopy (TEM) and nanoparticle tracking analysis (NTA). The components of proteins and miRNAs within these vesicles were identified by proteomic analysis and high-throughput small RNA sequencing. The biological function of targets of exosomal miRNAs was predicted by Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway analysis. Moreover, the expression of Th1- and Th2-type immune response associated cytokines in RAW264.7 macrophages were evaluated by qPCR and ELISA. We found that exosome-like vesicles were typical cup-shaped vesicles with diameters from 30 to 150 nm. A total of 87 proteins were identified by proteomic analysis, including proteins prominently associated with exosome-like vesicles biogenesis and vesicle trafficking. 41 known miRNAs and 18 novel miRNAs were identified in the exosome-like vesicles. Eleven selected miRNAs, including 7 known miRNAs (miR-71-5p, miR-10a-5p, miR-let-7-5p, miR-745-3p, miR-219-5p, miR-124-3p and miR-4989-3p) and 4 novel miRNAs (novel-mir-3, novel-mir-7, novel-mir-8 and novel-mir-11) were validated to exist in metacestiodes and exosome-like vesicles of T. Pisiformis cysticercus by qPCR. The functions of most targets of exosomal miRNAs were mainly associated with signal transduction and the immune system. Additionally, T. Pisiformis cysticercus-derived vesicles induced the production of IL-4, IL-6, IL-10, IL-13 and Arg-1, but downregulated the expression of IL-12, IFN-γ and iNOS in RAW264.7 macrophages. Conclusions We demonstrated that proteins and miRNAs enclosed within exosome-like vesicles from T. Pisiformis cysticercus have immunomodulatory functions. Furthermore, exosome-like vesicles were shown to induce the macrophage Th2-type immune response in vitro. Our study suggests that exosome-like vesicles play an important role in the interaction between cysticerci and their hosts.

  • Characterization of exosome-like vesicles derived from Taenia Pisiformis cysticercus and their immunoregulatory role on macrophages
    2020
    Co-Authors: Liqun Wang, Shaohua Zhang, Panhong Liang, Taoshan Li, Yanping Li
    Abstract:

    Abstract Background: Taenia Pisiformis is one of the most common intestinal parasites in canines, and leads to serious economic losses in the rabbit breeding industry. Exosome-like vesicles from parasites play crucial roles in host-parasite interactions by transferring cargo from parasites to host cells and by modulating host immunological response through inducing production of host-derived cytokines. Nevertheless, the mechanism by which exosome-like vesicles from T. Pisiformis cysticercus regulate the macrophage immune response remains unknown. Methods: Using ultracentrifugation, we isolated exosome-like vesicles from excretory/secretory products (ESP) of T. Pisiformis cysticercus. The morphology and size of purified vesicles were confirmed by transmission electron microscopy (TEM) and nanoparticle tracking analysis (NTA). The components of proteins and miRNAs within these vesicles were identified by proteomic analysis and high-throughput small RNA sequencing. The biological function of targets of exosomal miRNAs was predicted by Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway analysis. Moreover, the expression of Th1- and Th2-type immune response associated cytokines in RAW264.7 macrophages were evaluated by qPCR and ELISA. We found that exosome-like vesicles were typical cup-shaped vesicles with diameters from 30 to 150 nm. A total of 87 proteins were identified by proteomic analysis, including proteins prominently associated with exosome-like vesicles biogenesis and vesicle trafficking. 41 known miRNAs and 18 novel miRNAs were identified in the exosome-like vesicles. Eleven selected miRNAs, including 7 known miRNAs (miR-71-5p, miR-10a-5p, miR-let-7-5p, miR-745-3p, miR-219-5p, miR-124-3p and miR-4989-3p) and 4 novel miRNAs (novel-mir-3, novel-mir-7, novel-mir-8 and novel-mir-11) were validated to exist in metacestiodes and exosome-like vesicles of T. Pisiformis cysticercus by qPCR. The functions of most targets of exosomal miRNAs were mainly associated with signal transduction and the immune system. Additionally, T. Pisiformis cysticercus-derived vesicles induced the production of IL-4, IL-6, IL-10, IL-13 and Arg-1, but downregulated the expression of IL-12, IFN-γ and iNOS in RAW264.7 macrophages. Conclusions: We demonstrated that proteins and miRNAs enclosed within exosome-like vesicles from T. Pisiformis cysticercus have immunomodulatory functions. Furthermore, exosome-like vesicles were shown to induce the macrophage Th2-type immune response in vitro . Our study suggests that exosome-like vesicles play an important role in the interaction between cysticerci and their hosts. Keywords: Cysticercus Pisiformis, Exosome-like vesicles, Macrophages, Th2-type immune response.

  • screening and verification for proteins that interact with leucine aminopeptidase of Taenia Pisiformis using a yeast two hybrid system
    Parasitology Research, 2019
    Co-Authors: Shaohua Zhang
    Abstract:

    Leucine aminopeptidase of Taenia Pisiformis (TpLAP) belonging to the M17 peptidase family has been implicated as a stage-differentially expressed protein in the adult stage of T. Pisiformis. In order to further dissect the biological functions of TpLAP in the growth and development of adult worms, TpLAP-interacting partners were investigated. In this study, a yeast two-hybrid (Y2H) cDNA library from adult T. Pisiformis was constructed. Using pGBKT7-TpLAP as bait, proteins interacting with TpLAP were screened by Y2H system and positive preys were sequenced and analyzed using the Basic Local Alignment Search Tool (BLAST). Our results showed that six genuine TpLAP-interacting proteins, including LAP, dynein light chain (DLC), SUMO-conjugating enzyme (UBC9), histone-lysine n-methyltransferase, trans-acting transcriptional, and one unknown protein, were identified via Y2H assay. Furthermore, the interaction between TpLAP and UBC9 of T. Pisiformis (TpUBC9), an important protein involved in SUMOylation pathway, was further validated by one-to-one Y2H assay, co-immunoprecipitation, and confocal analysis. These findings provide a deeper understanding of the biological functions of TpLAP and offer the first clue that TpLAP may act as a novel SUMOylated substrate, suggesting that the SUMO modification pathway plays an important role in regulation of adult worm growth and development.

  • comparative transcriptomic analysis of the larval and adult stages of Taenia Pisiformis
    Genes, 2019
    Co-Authors: Shaohua Zhang
    Abstract:

    Taenia Pisiformis is a tapeworm causing economic losses in the rabbit breeding industry worldwide. Due to the absence of genomic data, our knowledge on the developmental process of T. Pisiformis is still inadequate. In this study, to better characterize differential and specific genes and pathways associated with the parasite developments, a comparative transcriptomic analysis of the larval stage (TpM) and the adult stage (TpA) of T. Pisiformis was performed by Illumina RNA sequencing (RNA-seq) technology and de novo analysis. In total, 68,588 unigenes were assembled with an average length of 789 nucleotides (nt) and N50 of 1485 nt. Further, we identified 4093 differentially expressed genes (DEGs) in TpA versus TpM, of which 3186 DEGs were upregulated and 907 were downregulated. Gene Ontology (GO) and Kyoto Encyclopedia of Genes (KEGG) analyses revealed that most DEGs involved in metabolic processes and Wnt signaling pathway were much more active in the TpA stage. Quantitative real-time PCR (qPCR) validated that the expression levels of the selected 10 DEGs were consistent with those in RNA-seq, indicating that the transcriptomic data are reliable. The present study provides comparative transcriptomic data concerning two developmental stages of T. Pisiformis, which will be of great value for future functional studies on the regulatory mechanisms behind adult worm pathogenesis and for developing drugs and vaccines against this important parasite.

Guangyou Yang - One of the best experts on this subject based on the ideXlab platform.

  • genetic characteristics of chinese isolates of the tapeworm Taenia Pisiformis based on two mitochondrial genes
    Journal of Helminthology, 2015
    Co-Authors: Deying Yang, Xiang Nong, Yan Fu, Xiaobin Gu, X Peng, Shuai Wang, Guangyou Yang
    Abstract:

    Cysticercosis is caused by infections with embryonated eggs of the tapeworm Taenia Pisiformis . Knowledge of the genetic characteristics of T. Pisiformis could be applied to study the epidemiology and transmission of this parasite. In this study, 61 isolates of intraperitoneal cysticerci from eight geographically distinct regions in Sichuan province, China, were subjected to a molecular analysis in order to determine their intra-regional genetic characteristics. Partial sequences of the mitochondrial cytochrome c oxidase subunit I ( cox1 , 1427 bp) and NADH dehydrogenase 1 ( nad1 , 738 bp) were concatenated. Five haplotypes were identified, and 89.04% of total genetic variation was found in collections of T. Pisiformis isolates from a single region. According to the phylogenetic reconstruction, the T. Pisiformis isolates from eight regions did not form geographical clusters. Our study highlights the genetic characteristics of T. Pisiformis with the aim of accelerating the genetic research and control of cysticercosis.

  • evaluation of a novel dot elisa assay utilizing a recombinant protein for the effective diagnosis of Taenia Pisiformis larval infections
    Veterinary Parasitology, 2014
    Co-Authors: Lin Chen, Deying Yang, Xiaobin Gu, Xuerong Peng, Guangyou Yang
    Abstract:

    Cysticercosis, caused by the larvae of Taenia Pisiformis, is a common disease in domestic breeds of the rabbit Oryctolagus cuniculus that results in economic losses. At present, there is no convenient and effective method for the rapid detection of T. Pisiformis larvae. Here, we developed and tested the efficacy of a Dot-ELISA assay for the diagnosis of T. Pisiformis larval infections in rabbits, based on the expression of the recombinant fusion protein (rTp1) from the Tp1 gene. Rapid amplification of cDNA ends (RACE) was used to amplify the 3′ ends of the Tp1 gene, based on the unigene similar to Ts1 gene (EU009656.1) which comes from transcriptome sequencing of T. Pisiformis. The Tp1 gene was successfully amplified, cloned and expressed in BL21 (DE3). Western blot analysis revealed that the recombinant Tp1 protein is specifically recognized by rabbit T. Pisiformis cysticercosis antisera. This purified recombinant fusion protein, rTp1, was probed by Dot-ELISA with sera from rabbits infected with T. Pisiformis larvae and with other parasitic infections. Results showed that this Dot-ELISA assay had both high sensitivity (92.9–97.6%) and specificity (95.2–98.4%) to detect T. Pisiformis larval infections. We also found very low levels of cross-reaction with other parasitic infections. This study has revealed that our novel Dot-ELISA assay utilizing the recombinant fusion protein, rTp1, has a strong potential for the effective diagnosis of T. Pisiformis infections in rabbits.

  • expression of the tpanxb1 gene from Taenia Pisiformis and its potential diagnostic value by dot elisa
    Journal of Parasitology, 2014
    Co-Authors: Deying Yang, Lin Chen, Xiang Nong, Xiaobin Gu, Xuhang Wu, Xuerong Peng, Xuan Zhou, Mei Li, Zuqin Chen, Guangyou Yang
    Abstract:

    Abstract:  Cysticercosis, caused by the larvae of Taenia Pisiformis, is a common disease in rabbits that results in economic losses. To date, there has been limited information available on the early detection of infection by this parasite. This study describes a dot-ELISA method based on an autologous antigen annexin B1 (Tpanxb1). Its potential for serodiagnosis of rabbit cysticercosis was also evaluated. Western blot analysis revealed that the recombinant Tpanxb1 (rTpanxb1) protein could be specifically recognized by rabbit anti-sera. In serum trials, the antibodies could be detected by dot-ELISA using rTpanxb1 at 14 days post-infection. The positive response was present for up to 49 days post-infection. Based on the necropsy results of 169 rabbit samples, the relative sensitivity and specificity of the dot-ELISA were 94.55% and 92.86%, respectively. This study provides a foundation for studying the immunological function of annexin and its application to control Taenia cestodes.

  • protection against Taenia Pisiformis larval infection induced by a recombinant oncosphere antigen vaccine
    Genetics and Molecular Research, 2014
    Co-Authors: Lin Chen, Deying Yang, Xiang Nong, Xing Huang, Yan Fu, Xiaobin Gu, Shuxian Wang, X Peng, Guangyou Yang
    Abstract:

    Taenia Pisiformis larvae cause significant health problems to rabbits. At present, it is not known whether the recombinant antigen from the T. Pisiformis oncosphere is able to confer protective immunity against T. Pisiformis larval infection. The full-length cDNA was cloned into a pET32a (+) vector, and the recombinant protein was then expressed in BL21 (DE3) cells. Vaccination with the purified rTpUbc2 coupled with QuilA was carried out in New Zealand rabbits to evaluate the immunoprotective effect against T. Pisiformis infection. The full-length open reading frame of the TpUbc2 gene was 444 bp, and encoded a 16.63-kDa protein. Finally, rTpUbc2 was used to evaluate the ability to induce immunoprotective responses in rabbits. A 79.3-90.8% reduction (P < 0.01) in the recovery of larvae was observed in the experimental group compared to the control group. Specific anti- rTpUbc2 antibodies from immunized rabbits had significantly higher levels of IgG (P < 0.01) compared to the control group; however, no significant difference in IgA levels was found between groups

  • genetic variation of Taenia Pisiformis collected from sichuan china based on the mitochondrial cytochrome b gene
    Korean Journal of Parasitology, 2013
    Co-Authors: Deying Yang, Xiang Nong, Yan Fu, Xiaobin Gu, Shuxian Wang, Xuerong Peng, Guangyou Yang
    Abstract:

    Taenia Pisiformis is one of the most important parasites of canines and rabbits. T. Pisiformis cysticercus (the larval stage) causes severe damage to rabbit breeding, which results in huge economic losses. In this study, the genetic variation of T. Pisiformis was determined in Sichuan Province, China. Fragments of the mitochondrial cytochrome b (cytb) (922 bp) gene were amplified in 53 isolates from 8 regions of T. Pisiformis. Overall, 12 haplotypes were found in these 53 cytb sequences. Molecular genetic variations showed 98.4% genetic variation derived from intra-region. FST and Nm values suggested that 53 isolates were not genetically differentiated and had low levels of genetic diversity. Neutrality indices of the cytb sequences showed the evolution of T. Pisiformis followed a neutral mode. Phylogenetic analysis revealed no correlation between phylogeny and geographic distribution. These findings indicate that 53 isolates of T. Pisiformis keep a low genetic variation, which provide useful knowledge for monitoring changes in parasite populations for future control strategies.

Lin Chen - One of the best experts on this subject based on the ideXlab platform.

  • comparative transcriptomes analysis of Taenia Pisiformis at different development stages
    bioRxiv, 2018
    Co-Authors: Lin Chen, Yu Jing, Jing Xu, Wei Wang, Lily Ji, Chengzhong Yang, Hua Yu
    Abstract:

    Abstract To understand the characteristics of the transcriptional group of Taenia Pisiformis at different developmental stages, and to lay the foundation for the screening of vaccine antigens and drug target genes, the transcriptomes of adult and larva of T. Pisiformis were assembled and analyzed using bioinformatic tools. A total of 36,951 unigenes with a mean length of 950bp were formed, among which 12,665, 8,188, 7,577, and 6,293 unigenes have been annotated respectively by sequence similarity analysis with four databases (NR, Swiss-Prot, KOG, and KEGG). It should be noted there are 5,662 unigenes that share good similarity with the four databases and get a relatively perfect functional annotation. Besides, a total of 10,247 differentially expressed genes were screened. To be specific, 6,910 unigenes were up-regulated in the larva stage while 3,337 were down-regulated in the adult stage. To sum up, this study sequenced and analyzed the transcriptomes of the larval and adult stages of T. Pisiformis. The results of differentially expressed genes in these two stages could provide basis for functional genomics, immunology and gene expression profiles of T. Pisiformis.

  • evaluation of a novel dot elisa assay utilizing a recombinant protein for the effective diagnosis of Taenia Pisiformis larval infections
    Veterinary Parasitology, 2014
    Co-Authors: Lin Chen, Deying Yang, Xiaobin Gu, Xuerong Peng, Guangyou Yang
    Abstract:

    Cysticercosis, caused by the larvae of Taenia Pisiformis, is a common disease in domestic breeds of the rabbit Oryctolagus cuniculus that results in economic losses. At present, there is no convenient and effective method for the rapid detection of T. Pisiformis larvae. Here, we developed and tested the efficacy of a Dot-ELISA assay for the diagnosis of T. Pisiformis larval infections in rabbits, based on the expression of the recombinant fusion protein (rTp1) from the Tp1 gene. Rapid amplification of cDNA ends (RACE) was used to amplify the 3′ ends of the Tp1 gene, based on the unigene similar to Ts1 gene (EU009656.1) which comes from transcriptome sequencing of T. Pisiformis. The Tp1 gene was successfully amplified, cloned and expressed in BL21 (DE3). Western blot analysis revealed that the recombinant Tp1 protein is specifically recognized by rabbit T. Pisiformis cysticercosis antisera. This purified recombinant fusion protein, rTp1, was probed by Dot-ELISA with sera from rabbits infected with T. Pisiformis larvae and with other parasitic infections. Results showed that this Dot-ELISA assay had both high sensitivity (92.9–97.6%) and specificity (95.2–98.4%) to detect T. Pisiformis larval infections. We also found very low levels of cross-reaction with other parasitic infections. This study has revealed that our novel Dot-ELISA assay utilizing the recombinant fusion protein, rTp1, has a strong potential for the effective diagnosis of T. Pisiformis infections in rabbits.

  • expression of the tpanxb1 gene from Taenia Pisiformis and its potential diagnostic value by dot elisa
    Journal of Parasitology, 2014
    Co-Authors: Deying Yang, Lin Chen, Xiang Nong, Xiaobin Gu, Xuhang Wu, Xuerong Peng, Xuan Zhou, Mei Li, Zuqin Chen, Guangyou Yang
    Abstract:

    Abstract:  Cysticercosis, caused by the larvae of Taenia Pisiformis, is a common disease in rabbits that results in economic losses. To date, there has been limited information available on the early detection of infection by this parasite. This study describes a dot-ELISA method based on an autologous antigen annexin B1 (Tpanxb1). Its potential for serodiagnosis of rabbit cysticercosis was also evaluated. Western blot analysis revealed that the recombinant Tpanxb1 (rTpanxb1) protein could be specifically recognized by rabbit anti-sera. In serum trials, the antibodies could be detected by dot-ELISA using rTpanxb1 at 14 days post-infection. The positive response was present for up to 49 days post-infection. Based on the necropsy results of 169 rabbit samples, the relative sensitivity and specificity of the dot-ELISA were 94.55% and 92.86%, respectively. This study provides a foundation for studying the immunological function of annexin and its application to control Taenia cestodes.

  • protection against Taenia Pisiformis larval infection induced by a recombinant oncosphere antigen vaccine
    Genetics and Molecular Research, 2014
    Co-Authors: Lin Chen, Deying Yang, Xiang Nong, Xing Huang, Yan Fu, Xiaobin Gu, Shuxian Wang, X Peng, Guangyou Yang
    Abstract:

    Taenia Pisiformis larvae cause significant health problems to rabbits. At present, it is not known whether the recombinant antigen from the T. Pisiformis oncosphere is able to confer protective immunity against T. Pisiformis larval infection. The full-length cDNA was cloned into a pET32a (+) vector, and the recombinant protein was then expressed in BL21 (DE3) cells. Vaccination with the purified rTpUbc2 coupled with QuilA was carried out in New Zealand rabbits to evaluate the immunoprotective effect against T. Pisiformis infection. The full-length open reading frame of the TpUbc2 gene was 444 bp, and encoded a 16.63-kDa protein. Finally, rTpUbc2 was used to evaluate the ability to induce immunoprotective responses in rabbits. A 79.3-90.8% reduction (P < 0.01) in the recovery of larvae was observed in the experimental group compared to the control group. Specific anti- rTpUbc2 antibodies from immunized rabbits had significantly higher levels of IgG (P < 0.01) compared to the control group; however, no significant difference in IgA levels was found between groups

  • analysis of codon usage patterns in Taenia Pisiformis through annotated transcriptome data
    Biochemical and Biophysical Research Communications, 2013
    Co-Authors: Lin Chen, Deying Yang, Xiang Nong, Xing Huang, Yan Fu, Xiaobin Gu, Shuxian Wang, Xuhang Wu, Xuerong Peng, Guangyou Yang
    Abstract:

    Abstract Taenia Pisiformis (Cestoidea; Cyclophyllidea; Taeniidae) tapeworms infect the small intestine of canids and felines, such as dogs and foxes. Synonymous codon usage in T. Pisiformis was examined through 8118 reconstructed annotations of transcriptome sequences. The mean value of GC content for the reconstructed genes was 49.48%. Twenty-four codons were determined as “optimal codons”. Approximately all translational optimal codons (except CGU) ended on G or C. The gene positions on the primary axis were strongly positively correlated with GC content at the third codon positions and GC content of individual genes. At the same time, the gene expression level assessed by the CAI, the hydrophobicity and aromaticity of encoded proteins were correlated with the GC content at the third codon positions and the effective number of codons (ENC), respectively. We infer that the gene expression level, the hydrophobicity and the aromaticity of the encoded proteins also influenced codon usage in T. Pisiformis . Knowledge of the codon usage pattern in T. Pisiformis can improve our understanding of the mechanisms of biased usage of synonymous codons and can help in selecting appropriate host expression systems for potential vaccine genes of T. Pisiformis.

Deying Yang - One of the best experts on this subject based on the ideXlab platform.

  • genetic characteristics of chinese isolates of the tapeworm Taenia Pisiformis based on two mitochondrial genes
    Journal of Helminthology, 2015
    Co-Authors: Deying Yang, Xiang Nong, Yan Fu, Xiaobin Gu, X Peng, Shuai Wang, Guangyou Yang
    Abstract:

    Cysticercosis is caused by infections with embryonated eggs of the tapeworm Taenia Pisiformis . Knowledge of the genetic characteristics of T. Pisiformis could be applied to study the epidemiology and transmission of this parasite. In this study, 61 isolates of intraperitoneal cysticerci from eight geographically distinct regions in Sichuan province, China, were subjected to a molecular analysis in order to determine their intra-regional genetic characteristics. Partial sequences of the mitochondrial cytochrome c oxidase subunit I ( cox1 , 1427 bp) and NADH dehydrogenase 1 ( nad1 , 738 bp) were concatenated. Five haplotypes were identified, and 89.04% of total genetic variation was found in collections of T. Pisiformis isolates from a single region. According to the phylogenetic reconstruction, the T. Pisiformis isolates from eight regions did not form geographical clusters. Our study highlights the genetic characteristics of T. Pisiformis with the aim of accelerating the genetic research and control of cysticercosis.

  • evaluation of a novel dot elisa assay utilizing a recombinant protein for the effective diagnosis of Taenia Pisiformis larval infections
    Veterinary Parasitology, 2014
    Co-Authors: Lin Chen, Deying Yang, Xiaobin Gu, Xuerong Peng, Guangyou Yang
    Abstract:

    Cysticercosis, caused by the larvae of Taenia Pisiformis, is a common disease in domestic breeds of the rabbit Oryctolagus cuniculus that results in economic losses. At present, there is no convenient and effective method for the rapid detection of T. Pisiformis larvae. Here, we developed and tested the efficacy of a Dot-ELISA assay for the diagnosis of T. Pisiformis larval infections in rabbits, based on the expression of the recombinant fusion protein (rTp1) from the Tp1 gene. Rapid amplification of cDNA ends (RACE) was used to amplify the 3′ ends of the Tp1 gene, based on the unigene similar to Ts1 gene (EU009656.1) which comes from transcriptome sequencing of T. Pisiformis. The Tp1 gene was successfully amplified, cloned and expressed in BL21 (DE3). Western blot analysis revealed that the recombinant Tp1 protein is specifically recognized by rabbit T. Pisiformis cysticercosis antisera. This purified recombinant fusion protein, rTp1, was probed by Dot-ELISA with sera from rabbits infected with T. Pisiformis larvae and with other parasitic infections. Results showed that this Dot-ELISA assay had both high sensitivity (92.9–97.6%) and specificity (95.2–98.4%) to detect T. Pisiformis larval infections. We also found very low levels of cross-reaction with other parasitic infections. This study has revealed that our novel Dot-ELISA assay utilizing the recombinant fusion protein, rTp1, has a strong potential for the effective diagnosis of T. Pisiformis infections in rabbits.

  • expression of the tpanxb1 gene from Taenia Pisiformis and its potential diagnostic value by dot elisa
    Journal of Parasitology, 2014
    Co-Authors: Deying Yang, Lin Chen, Xiang Nong, Xiaobin Gu, Xuhang Wu, Xuerong Peng, Xuan Zhou, Mei Li, Zuqin Chen, Guangyou Yang
    Abstract:

    Abstract:  Cysticercosis, caused by the larvae of Taenia Pisiformis, is a common disease in rabbits that results in economic losses. To date, there has been limited information available on the early detection of infection by this parasite. This study describes a dot-ELISA method based on an autologous antigen annexin B1 (Tpanxb1). Its potential for serodiagnosis of rabbit cysticercosis was also evaluated. Western blot analysis revealed that the recombinant Tpanxb1 (rTpanxb1) protein could be specifically recognized by rabbit anti-sera. In serum trials, the antibodies could be detected by dot-ELISA using rTpanxb1 at 14 days post-infection. The positive response was present for up to 49 days post-infection. Based on the necropsy results of 169 rabbit samples, the relative sensitivity and specificity of the dot-ELISA were 94.55% and 92.86%, respectively. This study provides a foundation for studying the immunological function of annexin and its application to control Taenia cestodes.

  • protection against Taenia Pisiformis larval infection induced by a recombinant oncosphere antigen vaccine
    Genetics and Molecular Research, 2014
    Co-Authors: Lin Chen, Deying Yang, Xiang Nong, Xing Huang, Yan Fu, Xiaobin Gu, Shuxian Wang, X Peng, Guangyou Yang
    Abstract:

    Taenia Pisiformis larvae cause significant health problems to rabbits. At present, it is not known whether the recombinant antigen from the T. Pisiformis oncosphere is able to confer protective immunity against T. Pisiformis larval infection. The full-length cDNA was cloned into a pET32a (+) vector, and the recombinant protein was then expressed in BL21 (DE3) cells. Vaccination with the purified rTpUbc2 coupled with QuilA was carried out in New Zealand rabbits to evaluate the immunoprotective effect against T. Pisiformis infection. The full-length open reading frame of the TpUbc2 gene was 444 bp, and encoded a 16.63-kDa protein. Finally, rTpUbc2 was used to evaluate the ability to induce immunoprotective responses in rabbits. A 79.3-90.8% reduction (P < 0.01) in the recovery of larvae was observed in the experimental group compared to the control group. Specific anti- rTpUbc2 antibodies from immunized rabbits had significantly higher levels of IgG (P < 0.01) compared to the control group; however, no significant difference in IgA levels was found between groups

  • genetic variation of Taenia Pisiformis collected from sichuan china based on the mitochondrial cytochrome b gene
    Korean Journal of Parasitology, 2013
    Co-Authors: Deying Yang, Xiang Nong, Yan Fu, Xiaobin Gu, Shuxian Wang, Xuerong Peng, Guangyou Yang
    Abstract:

    Taenia Pisiformis is one of the most important parasites of canines and rabbits. T. Pisiformis cysticercus (the larval stage) causes severe damage to rabbit breeding, which results in huge economic losses. In this study, the genetic variation of T. Pisiformis was determined in Sichuan Province, China. Fragments of the mitochondrial cytochrome b (cytb) (922 bp) gene were amplified in 53 isolates from 8 regions of T. Pisiformis. Overall, 12 haplotypes were found in these 53 cytb sequences. Molecular genetic variations showed 98.4% genetic variation derived from intra-region. FST and Nm values suggested that 53 isolates were not genetically differentiated and had low levels of genetic diversity. Neutrality indices of the cytb sequences showed the evolution of T. Pisiformis followed a neutral mode. Phylogenetic analysis revealed no correlation between phylogeny and geographic distribution. These findings indicate that 53 isolates of T. Pisiformis keep a low genetic variation, which provide useful knowledge for monitoring changes in parasite populations for future control strategies.

Xiaobin Gu - One of the best experts on this subject based on the ideXlab platform.

  • genetic characteristics of chinese isolates of the tapeworm Taenia Pisiformis based on two mitochondrial genes
    Journal of Helminthology, 2015
    Co-Authors: Deying Yang, Xiang Nong, Yan Fu, Xiaobin Gu, X Peng, Shuai Wang, Guangyou Yang
    Abstract:

    Cysticercosis is caused by infections with embryonated eggs of the tapeworm Taenia Pisiformis . Knowledge of the genetic characteristics of T. Pisiformis could be applied to study the epidemiology and transmission of this parasite. In this study, 61 isolates of intraperitoneal cysticerci from eight geographically distinct regions in Sichuan province, China, were subjected to a molecular analysis in order to determine their intra-regional genetic characteristics. Partial sequences of the mitochondrial cytochrome c oxidase subunit I ( cox1 , 1427 bp) and NADH dehydrogenase 1 ( nad1 , 738 bp) were concatenated. Five haplotypes were identified, and 89.04% of total genetic variation was found in collections of T. Pisiformis isolates from a single region. According to the phylogenetic reconstruction, the T. Pisiformis isolates from eight regions did not form geographical clusters. Our study highlights the genetic characteristics of T. Pisiformis with the aim of accelerating the genetic research and control of cysticercosis.

  • evaluation of a novel dot elisa assay utilizing a recombinant protein for the effective diagnosis of Taenia Pisiformis larval infections
    Veterinary Parasitology, 2014
    Co-Authors: Lin Chen, Deying Yang, Xiaobin Gu, Xuerong Peng, Guangyou Yang
    Abstract:

    Cysticercosis, caused by the larvae of Taenia Pisiformis, is a common disease in domestic breeds of the rabbit Oryctolagus cuniculus that results in economic losses. At present, there is no convenient and effective method for the rapid detection of T. Pisiformis larvae. Here, we developed and tested the efficacy of a Dot-ELISA assay for the diagnosis of T. Pisiformis larval infections in rabbits, based on the expression of the recombinant fusion protein (rTp1) from the Tp1 gene. Rapid amplification of cDNA ends (RACE) was used to amplify the 3′ ends of the Tp1 gene, based on the unigene similar to Ts1 gene (EU009656.1) which comes from transcriptome sequencing of T. Pisiformis. The Tp1 gene was successfully amplified, cloned and expressed in BL21 (DE3). Western blot analysis revealed that the recombinant Tp1 protein is specifically recognized by rabbit T. Pisiformis cysticercosis antisera. This purified recombinant fusion protein, rTp1, was probed by Dot-ELISA with sera from rabbits infected with T. Pisiformis larvae and with other parasitic infections. Results showed that this Dot-ELISA assay had both high sensitivity (92.9–97.6%) and specificity (95.2–98.4%) to detect T. Pisiformis larval infections. We also found very low levels of cross-reaction with other parasitic infections. This study has revealed that our novel Dot-ELISA assay utilizing the recombinant fusion protein, rTp1, has a strong potential for the effective diagnosis of T. Pisiformis infections in rabbits.

  • expression of the tpanxb1 gene from Taenia Pisiformis and its potential diagnostic value by dot elisa
    Journal of Parasitology, 2014
    Co-Authors: Deying Yang, Lin Chen, Xiang Nong, Xiaobin Gu, Xuhang Wu, Xuerong Peng, Xuan Zhou, Mei Li, Zuqin Chen, Guangyou Yang
    Abstract:

    Abstract:  Cysticercosis, caused by the larvae of Taenia Pisiformis, is a common disease in rabbits that results in economic losses. To date, there has been limited information available on the early detection of infection by this parasite. This study describes a dot-ELISA method based on an autologous antigen annexin B1 (Tpanxb1). Its potential for serodiagnosis of rabbit cysticercosis was also evaluated. Western blot analysis revealed that the recombinant Tpanxb1 (rTpanxb1) protein could be specifically recognized by rabbit anti-sera. In serum trials, the antibodies could be detected by dot-ELISA using rTpanxb1 at 14 days post-infection. The positive response was present for up to 49 days post-infection. Based on the necropsy results of 169 rabbit samples, the relative sensitivity and specificity of the dot-ELISA were 94.55% and 92.86%, respectively. This study provides a foundation for studying the immunological function of annexin and its application to control Taenia cestodes.

  • protection against Taenia Pisiformis larval infection induced by a recombinant oncosphere antigen vaccine
    Genetics and Molecular Research, 2014
    Co-Authors: Lin Chen, Deying Yang, Xiang Nong, Xing Huang, Yan Fu, Xiaobin Gu, Shuxian Wang, X Peng, Guangyou Yang
    Abstract:

    Taenia Pisiformis larvae cause significant health problems to rabbits. At present, it is not known whether the recombinant antigen from the T. Pisiformis oncosphere is able to confer protective immunity against T. Pisiformis larval infection. The full-length cDNA was cloned into a pET32a (+) vector, and the recombinant protein was then expressed in BL21 (DE3) cells. Vaccination with the purified rTpUbc2 coupled with QuilA was carried out in New Zealand rabbits to evaluate the immunoprotective effect against T. Pisiformis infection. The full-length open reading frame of the TpUbc2 gene was 444 bp, and encoded a 16.63-kDa protein. Finally, rTpUbc2 was used to evaluate the ability to induce immunoprotective responses in rabbits. A 79.3-90.8% reduction (P < 0.01) in the recovery of larvae was observed in the experimental group compared to the control group. Specific anti- rTpUbc2 antibodies from immunized rabbits had significantly higher levels of IgG (P < 0.01) compared to the control group; however, no significant difference in IgA levels was found between groups

  • genetic variation of Taenia Pisiformis collected from sichuan china based on the mitochondrial cytochrome b gene
    Korean Journal of Parasitology, 2013
    Co-Authors: Deying Yang, Xiang Nong, Yan Fu, Xiaobin Gu, Shuxian Wang, Xuerong Peng, Guangyou Yang
    Abstract:

    Taenia Pisiformis is one of the most important parasites of canines and rabbits. T. Pisiformis cysticercus (the larval stage) causes severe damage to rabbit breeding, which results in huge economic losses. In this study, the genetic variation of T. Pisiformis was determined in Sichuan Province, China. Fragments of the mitochondrial cytochrome b (cytb) (922 bp) gene were amplified in 53 isolates from 8 regions of T. Pisiformis. Overall, 12 haplotypes were found in these 53 cytb sequences. Molecular genetic variations showed 98.4% genetic variation derived from intra-region. FST and Nm values suggested that 53 isolates were not genetically differentiated and had low levels of genetic diversity. Neutrality indices of the cytb sequences showed the evolution of T. Pisiformis followed a neutral mode. Phylogenetic analysis revealed no correlation between phylogeny and geographic distribution. These findings indicate that 53 isolates of T. Pisiformis keep a low genetic variation, which provide useful knowledge for monitoring changes in parasite populations for future control strategies.