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Badiaa Lyoussi - One of the best experts on this subject based on the ideXlab platform.
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Vascular effects of Tanacetum Vulgare L. leaf extract: In vitro pharmacological study
Journal of ethnopharmacology, 2008Co-Authors: Sanaa Lahlou, Khadija Cherkaoui Tangi, Badiaa Lyoussi, Nicole MorelAbstract:Aim of the study: Tanacetum Vulgare L. (Asteraceae/Compositae) is principally used in traditional Moroccan medicine as anti hypertensive remedy. The aim of the present study was to investigate the in vitro vascular activity of the aqueous extract of Tanacetum Vulgare L Materials and Methods: The activity of Tanacetum Vulgare L. extract was tested on contractile response of Wistar rat aorta to high KCl and noradrenaline and on endothelium-dependent relaxation evoked by acetylcholine. Results: The addition of Tanacetum extract during the plateau phase of noradrenaline-evoked contraction produced a rapid relaxation that reached a maximum of 30% of the contraction and was suppressed by the NO synthase inhibitor N-G-nitro-L-arginine. At higher extract concentrations this rapid relaxation was followed by a slowly developing, N-G-nitro-L-arginine-resistant, relaxing effect. Tanacetum extract also depressed KCl-evoked contraction by 30% at maximum. This effect was abolished in the presence of N-G-nitro-L-arginine. The endothelium-dependent relaxation induced by acetylcholine was depressed in the presence of Tanacetum extract in the bathing solution. Conclusion: :This study indicates that the aqueous extract of Tanacetum possesses NO-mediated and NO-independent vasorelaxing properties in vitro. (C) 2008 Elsevier Ireland Ltd. All rights reserved.
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acute and chronic toxicity of a lyophilised aqueous extract of Tanacetum Vulgare leaves in rodents
Journal of Ethnopharmacology, 2008Co-Authors: Sanaa Lahlou, Zafar H Israili, Badiaa LyoussiAbstract:Abstract Aim of the study The present investigation was carried out to evaluate the safety of an aqueous extract of tansy (Tanacetum Vulgare L.) leaves by determining its potential toxicity after acute and chronic administration in rodents. Materials and Methods For the acute study, a lyophilized aqueous extract of tansy leaves was administered to mice in single doses of 0-13 g/kg given by gavage as well as intraperitoneal doses of 0–4.5 g/kg. General behavior adverse effects and mortality were determined for up to 14 days. In the chronic dose study, the extract was administered orally at doses of 0, 100, 300 and 600 mg/kg daily for 90 days to rats. Biochemical and hematological parameters were determined after 30 and 60 days, and then at the end of 90 days of daily administration. Results In the acute study in mice, the crude aqueous extract of tansy leaves caused dose-dependent general behavior adverse effects and mortality. The no-observed adverse effect levels (NOAEL) of the tansy extract were 7.0 g/kg and 1.0 g/kg, and the lowest-observed adverse effect levels (LOAEL) were 9.0 g/kg and 1.5 g/kg, when given by the oral and intraperitoneal routes, respectively. Mortality increased with increasing doses, with LD 50 of 9.9 g/kg and 2.8 g/kg for the oral and intraperitonal modes of administration, respectively. In the chronic study in rats, daily oral administration of the crude aqueous extract of tansy leaves for up to 90 days did not result in death or significant changes in the biological (except for hypoglycemia) and hematological parameters. Conclusions Because of the relatively high NOAEL values in the acute study in mice, and lack of significant effect on biological and hematological parameters in rats after 90 days of daily doses, the tansy extract does not appear to have significant toxicity. In view of the dose of tansy consumed in traditional medicine, there is a wide margin of safety for the therapeutic use of the aqueous extract of Tanacetum Vulgare leaves.
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diuretic activity of the aqueous extracts of carum carvi and Tanacetum Vulgare in normal rats
Journal of Ethnopharmacology, 2007Co-Authors: Sanaa Lahlou, Adil Tahraoui, Zafar H Israili, Badiaa LyoussiAbstract:In the Moroccan traditional medicine, the ripe fruits of Carum carvi L. (Apiaceae) and the leaves of Tanacetum Vulgare L. (Asteraceae/Compositae), two widely available plant materials, are used as diuretics. Since, the diuretic activity of these substances has not been investigated in scientifically controlled studies, the aim of the present study was to evaluate the diuretic potential of aqueous extracts of Carum carvi fruit (caraway) and the leaves of Tanacetum Vulgare (tansy) in normal rats after acute and sub-chronic oral administration. Water extracts of Carum carvi and Tanacetum Vulgare (100 mg/kg) or the reference drug, furosemide (10 mg/kg) were administrated orally to male Wistar rats and their urine output was quantitated at several intervals of time after the dose. After single doses of the extracts of both caraway seeds and tansy leaves, urine output was significantly increased at all time points, and at 24 h after the dose, the total volume of urine excreted was similar for the plant extracts and furosemide. Both extracts increased urinary levels of Na(+) and K(+), to about the same extent, while furosemide increased urinary levels of only Na(+) and decreased urinary K(+). Despite changes in urinary excretion of the electrolytes, plasma Na(+) and K(+) levels were not affected by any of the three substances. In the 8-day sub-chronic study, all three substances induced significant diuresis and natriuresis; only tansy increased urinary potassium excretion. The plant extracts did not appear to have renal toxicity or any other adverse effects during the study period. In conclusion, water extracts of both Carum carvi and Tanacetum Vulgare have strong diuretic action confirming their ethnopharmacological use. From the pattern of excretion of water, sodium and potassium, it may be deduced that there are atleast two types of active principals present in these extracts, one having a furosemide-like activity and the other a thiazide-like activity.
Francisco Parra - One of the best experts on this subject based on the ideXlab platform.
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a spiroketal enol ether derivative from Tanacetum Vulgare selectively inhibits hsv 1 and hsv 2 glycoprotein accumulation in vero cells
Antiviral Research, 2015Co-Authors: Ángel L. Álvarez, Santiago Melon, Kevin P Dalton, Solomon Habtemariam, Ahmed Abdel E. Moneim, Francisco ParraAbstract:The inhibitory effects of Tanacetum Vulgare rhizome extracts on HSV-1 and HSV-2 in vitro replication were assessed. Unlike extracts obtained from the aerial parts, adsorption inhibition and virucidal activities seemed not to be relevant for the observed antiviral action of tansy rhizome extracts. Instead, the most significant effects were the inhibition of virus penetration and a novel mechanism consisting of the specific arrest of viral gene expression and consequently the decrease of viral protein accumulation within infected cells. Through a bioactivity-guided fractionation protocol we isolated and identified the spiroketal-enol ether derivative (E)-2-(2,4-hexadiynyliden)-1,6-dioxaspiro[4.5]dec-3-ene as the active compound responsible for this inhibitory effect.
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in vitro anti hsv 1 and hsv 2 activity of Tanacetum Vulgare extracts and isolated compounds an approach to their mechanisms of action
Phytotherapy Research, 2010Co-Authors: Ángel L. Álvarez, Solomon Habtemariam, Malindra Juanbadaturuge, Caroline Jackson, Francisco ParraAbstract:Herpes simplex viruses (HSV-1 and HSV-2) are responsible for long-term latent infections in humans, with periods of recurring viral replication associated to lesions around the lips, eyes, mucous membrane of the oral cavity or the genitals. The lack of an effective vaccine, the moderate to high toxicity of the available synthetic antiherpes compounds and the appearance of resistant viral strains emphasize the need for new inhibitors. Tanacetum Vulgare, commonly known as tansy, has been used for treating rheumatic pain, skin eruption and diuretic conditions as well as an anthelmintic, antihypertensive, stimulant, emmenagogue, carminative, antiseptic, antihypertensive, antispasmodic and antioxidant agent. The anti HSV-1 activity of tansy aerial parts, ethyl acetate extract and the isolated compound parthenolide, has been reported recently. In this work, through a comprehensive mechanistic-based antiherpetic activity study, it was revealed that constituents other than parthenolide are responsible for the antiviral activity of tansy.
Sanaa Lahlou - One of the best experts on this subject based on the ideXlab platform.
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Vascular effects of Tanacetum Vulgare L. leaf extract: In vitro pharmacological study
Journal of ethnopharmacology, 2008Co-Authors: Sanaa Lahlou, Khadija Cherkaoui Tangi, Badiaa Lyoussi, Nicole MorelAbstract:Aim of the study: Tanacetum Vulgare L. (Asteraceae/Compositae) is principally used in traditional Moroccan medicine as anti hypertensive remedy. The aim of the present study was to investigate the in vitro vascular activity of the aqueous extract of Tanacetum Vulgare L Materials and Methods: The activity of Tanacetum Vulgare L. extract was tested on contractile response of Wistar rat aorta to high KCl and noradrenaline and on endothelium-dependent relaxation evoked by acetylcholine. Results: The addition of Tanacetum extract during the plateau phase of noradrenaline-evoked contraction produced a rapid relaxation that reached a maximum of 30% of the contraction and was suppressed by the NO synthase inhibitor N-G-nitro-L-arginine. At higher extract concentrations this rapid relaxation was followed by a slowly developing, N-G-nitro-L-arginine-resistant, relaxing effect. Tanacetum extract also depressed KCl-evoked contraction by 30% at maximum. This effect was abolished in the presence of N-G-nitro-L-arginine. The endothelium-dependent relaxation induced by acetylcholine was depressed in the presence of Tanacetum extract in the bathing solution. Conclusion: :This study indicates that the aqueous extract of Tanacetum possesses NO-mediated and NO-independent vasorelaxing properties in vitro. (C) 2008 Elsevier Ireland Ltd. All rights reserved.
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acute and chronic toxicity of a lyophilised aqueous extract of Tanacetum Vulgare leaves in rodents
Journal of Ethnopharmacology, 2008Co-Authors: Sanaa Lahlou, Zafar H Israili, Badiaa LyoussiAbstract:Abstract Aim of the study The present investigation was carried out to evaluate the safety of an aqueous extract of tansy (Tanacetum Vulgare L.) leaves by determining its potential toxicity after acute and chronic administration in rodents. Materials and Methods For the acute study, a lyophilized aqueous extract of tansy leaves was administered to mice in single doses of 0-13 g/kg given by gavage as well as intraperitoneal doses of 0–4.5 g/kg. General behavior adverse effects and mortality were determined for up to 14 days. In the chronic dose study, the extract was administered orally at doses of 0, 100, 300 and 600 mg/kg daily for 90 days to rats. Biochemical and hematological parameters were determined after 30 and 60 days, and then at the end of 90 days of daily administration. Results In the acute study in mice, the crude aqueous extract of tansy leaves caused dose-dependent general behavior adverse effects and mortality. The no-observed adverse effect levels (NOAEL) of the tansy extract were 7.0 g/kg and 1.0 g/kg, and the lowest-observed adverse effect levels (LOAEL) were 9.0 g/kg and 1.5 g/kg, when given by the oral and intraperitoneal routes, respectively. Mortality increased with increasing doses, with LD 50 of 9.9 g/kg and 2.8 g/kg for the oral and intraperitonal modes of administration, respectively. In the chronic study in rats, daily oral administration of the crude aqueous extract of tansy leaves for up to 90 days did not result in death or significant changes in the biological (except for hypoglycemia) and hematological parameters. Conclusions Because of the relatively high NOAEL values in the acute study in mice, and lack of significant effect on biological and hematological parameters in rats after 90 days of daily doses, the tansy extract does not appear to have significant toxicity. In view of the dose of tansy consumed in traditional medicine, there is a wide margin of safety for the therapeutic use of the aqueous extract of Tanacetum Vulgare leaves.
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diuretic activity of the aqueous extracts of carum carvi and Tanacetum Vulgare in normal rats
Journal of Ethnopharmacology, 2007Co-Authors: Sanaa Lahlou, Adil Tahraoui, Zafar H Israili, Badiaa LyoussiAbstract:In the Moroccan traditional medicine, the ripe fruits of Carum carvi L. (Apiaceae) and the leaves of Tanacetum Vulgare L. (Asteraceae/Compositae), two widely available plant materials, are used as diuretics. Since, the diuretic activity of these substances has not been investigated in scientifically controlled studies, the aim of the present study was to evaluate the diuretic potential of aqueous extracts of Carum carvi fruit (caraway) and the leaves of Tanacetum Vulgare (tansy) in normal rats after acute and sub-chronic oral administration. Water extracts of Carum carvi and Tanacetum Vulgare (100 mg/kg) or the reference drug, furosemide (10 mg/kg) were administrated orally to male Wistar rats and their urine output was quantitated at several intervals of time after the dose. After single doses of the extracts of both caraway seeds and tansy leaves, urine output was significantly increased at all time points, and at 24 h after the dose, the total volume of urine excreted was similar for the plant extracts and furosemide. Both extracts increased urinary levels of Na(+) and K(+), to about the same extent, while furosemide increased urinary levels of only Na(+) and decreased urinary K(+). Despite changes in urinary excretion of the electrolytes, plasma Na(+) and K(+) levels were not affected by any of the three substances. In the 8-day sub-chronic study, all three substances induced significant diuresis and natriuresis; only tansy increased urinary potassium excretion. The plant extracts did not appear to have renal toxicity or any other adverse effects during the study period. In conclusion, water extracts of both Carum carvi and Tanacetum Vulgare have strong diuretic action confirming their ethnopharmacological use. From the pattern of excretion of water, sodium and potassium, it may be deduced that there are atleast two types of active principals present in these extracts, one having a furosemide-like activity and the other a thiazide-like activity.
Celso Vataru Nakamura - One of the best experts on this subject based on the ideXlab platform.
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Tanacetum Vulgare antiherpes virus activity of crude extract and the purified compound parthenolide
Phytotherapy Research, 2009Co-Authors: Thelma Onozato, Benedito Prado Dias Filho, Diógenes Aparício Garcia Cortez, Celso Vataru Nakamura, Tânia UedanakamuraAbstract:The present study demonstrated that the ethyl acetate extract and the isolated compound, parthenolide, from aerial parts of Tanacetum Vulgare L. (Asteraceae), protected Vero cells from herpes simplex virus (HSV-1) infection in vitro. The extract and parthenolide were assayed against HSV-1 by the sulforhodamine B colorimetric assay and exhibited anti-HSV-1 activity with an EC50 of 40 µg/mL and 0.3 µg/mL, respectively. In order to determine which stage of the virus–cell interaction was affected by parthenolide, the pure compound was used. No effect was observed when both viruses and cells were pretreated, or during early stages of infection, suggesting that parthenolide interfered with virus replication after the penetration stage, inhibiting approximately 40% of plaques formed at a concentration of 2.5 µg/mL when compared with an untreated control. Copyright © 2009 John Wiley & Sons, Ltd.
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efeito de extratos de plantas medicinais no crescimento de leishmania l amazonensis e trypanosoma cruzi
Revista Brasileira De Ciencias Farmaceuticas, 2005Co-Authors: Patrícia Shima Luize, Tatiana Shioji Tiuman, Luis Gustavo Morello, Paloma Korehiza Maza, Benedito Prado Dias Filho, Diógenes Aparício Garcia Cortez, João Carlos Palazzo De Mello, Tânia Uedanakamura, Celso Vataru NakamuraAbstract:This study describes the screening of extracts obtained from 19 species of plants used in Brazilian traditional medicine for treatment of a variety of diseases. The extracts were tested against axenic amastigote and promastigote forms of Leishmania (L.) amazonensis, and epimastigote forms of Trypanosoma cruzi in vitro at a concentration of 100 mg/ml. Baccharis trimera, Cymbopogon citratus, Matricaria chamomilla, Mikania glomerata, Ocimum gratissimum, Piper regnellii, Prunus domestica, Psidium guajava, Sambucus canadensis, Stryphnodendron adstringens, Tanacetum parthenium, and Tanacetum Vulgare showed significant effects against one or both parasites, with a percentage of growth inhibition between 49.5 and 99%. The extracts showed no cytotoxic effect on sheep erythrocytes. These medicinal plants may be sources of new compounds that are clinically active against L. amazonensis and T. cruzi.
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Effect of plant extracts used in folk medicine on cell growth and differentiation of Herpetomonas samuelpessoai (Kinetoplastida, Trypanosomatidae) cultivated in defined medium Efeito de extratos de plantas utilizadas na medicina popular no cresciment
Eduem - Editora da Universidade Estadual de Maringá, 2002Co-Authors: Fabiola Barbieri Holetz, Benedito Prado Dias Filho, Diógenes Aparício Garcia Cortez, João Carlos Palazzo De Mello, Tania Ueda Nakamura, Celso Vataru NakamuraAbstract:This work reports the effect of 15 medicinal plants on cell growth and differentiation of Herpetomonas samuelpessoai, a non-pathogenic trypanosomatid, used as biological model for its similar antigens to Trypanosoma cruzi. Crude extracts (1,000 g/ml) or essential oil (250 g/ml) were added in a defined medium. Cell growth was estimated by counting in Neubauer’s chamber and cell differentiation was examined by light microscope. Ocimum gratissimum, Lippia alba, Piper regnellii, Stryphnodendron adstringens, and Tanacetum Vulgare showed antiprotozoan activity, Psidium guajava and Punica granatum a lower activity and Achillea millefolium, Eugenia uniflora, Mikania glomerata, Plantago major, and Spilanthes acmella had no activity. In contrast, Arctium lappa, Erythrina speciosa, and Sambucus Canadensis stimulated H. samuelpessoai growth. Only L. alba and S. acmella stimulated cell differentiation in this flagellate. These results indicate that medicinal plants possess active compounds against H. samuelpessoai. Thus, this protozoan seems to be a suitable model for screening plants containing trypanocidal drugsNeste trabalho, verificou-se o efeito de 15 plantas medicinais no crescimento e diferenciação celular de Herpetomonas samuelpessoai, um tripanosomatídeo não patogênico utilizado como modelo biológico, que apresenta antígenos semelhantes aos do Trypanosoma cruzi. Extratos brutos (1.000 g/ml) ou óleo essencial (250 µg/ml) foram adicionados ao meio definido. O crescimento celular foi determinado pela contagem em câmara de Newbauer e a diferenciação celular examinada por microscopia ótica. Ocimum gratissimum, Lippia alba, Piper regnellii, Stryphnodendron adstringens, e Tanacetum Vulgare mostraram atividade antiprotozoário, Psidium guajava e Punica granatum menor atividade e Achillea millefolium, Eugenia uniflora, Mikania glomerata, Plantago major, e Spilanthes acmella não apresentaram atividade. Por outro lado, Arctium lappa, Erythrina speciosa, e Sambucus canadensis estimularam o crescimento de H. samuelpessoai e L. alba e S. acmella a diferenciação celular deste flagelado. Estes resultados indicam que plantas medicinais possuem princípios ativos contra H. samuelpessoai, o qual parece ser útil como modelo para seleção de plantas que contém drogas tripanomicida
Tânia Uedanakamura - One of the best experts on this subject based on the ideXlab platform.
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Tanacetum Vulgare antiherpes virus activity of crude extract and the purified compound parthenolide
Phytotherapy Research, 2009Co-Authors: Thelma Onozato, Benedito Prado Dias Filho, Diógenes Aparício Garcia Cortez, Celso Vataru Nakamura, Tânia UedanakamuraAbstract:The present study demonstrated that the ethyl acetate extract and the isolated compound, parthenolide, from aerial parts of Tanacetum Vulgare L. (Asteraceae), protected Vero cells from herpes simplex virus (HSV-1) infection in vitro. The extract and parthenolide were assayed against HSV-1 by the sulforhodamine B colorimetric assay and exhibited anti-HSV-1 activity with an EC50 of 40 µg/mL and 0.3 µg/mL, respectively. In order to determine which stage of the virus–cell interaction was affected by parthenolide, the pure compound was used. No effect was observed when both viruses and cells were pretreated, or during early stages of infection, suggesting that parthenolide interfered with virus replication after the penetration stage, inhibiting approximately 40% of plaques formed at a concentration of 2.5 µg/mL when compared with an untreated control. Copyright © 2009 John Wiley & Sons, Ltd.
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efeito de extratos de plantas medicinais no crescimento de leishmania l amazonensis e trypanosoma cruzi
Revista Brasileira De Ciencias Farmaceuticas, 2005Co-Authors: Patrícia Shima Luize, Tatiana Shioji Tiuman, Luis Gustavo Morello, Paloma Korehiza Maza, Benedito Prado Dias Filho, Diógenes Aparício Garcia Cortez, João Carlos Palazzo De Mello, Tânia Uedanakamura, Celso Vataru NakamuraAbstract:This study describes the screening of extracts obtained from 19 species of plants used in Brazilian traditional medicine for treatment of a variety of diseases. The extracts were tested against axenic amastigote and promastigote forms of Leishmania (L.) amazonensis, and epimastigote forms of Trypanosoma cruzi in vitro at a concentration of 100 mg/ml. Baccharis trimera, Cymbopogon citratus, Matricaria chamomilla, Mikania glomerata, Ocimum gratissimum, Piper regnellii, Prunus domestica, Psidium guajava, Sambucus canadensis, Stryphnodendron adstringens, Tanacetum parthenium, and Tanacetum Vulgare showed significant effects against one or both parasites, with a percentage of growth inhibition between 49.5 and 99%. The extracts showed no cytotoxic effect on sheep erythrocytes. These medicinal plants may be sources of new compounds that are clinically active against L. amazonensis and T. cruzi.
