The Experts below are selected from a list of 1404 Experts worldwide ranked by ideXlab platform
Mirko Manetti - One of the best experts on this subject based on the ideXlab platform.
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Telocytes constitute a widespread interstitial meshwork in the lamina propria and underlying striated muscle of human tongue
Nature Publishing Group, 2019Co-Authors: Irene Rosa, Lidia Ibba-manneschi, Mirca Marini, Cecilia Taverna, Luca Novelli, Mirko ManettiAbstract:Abstract Telocytes have recently emerged as unique interstitial cells defined by their extremely long, thin and moniliform prolongations termed telopodes. Despite growing evidence that these cells consistently reside in the stromal compartment of various organs from human beings, studies dealing with Telocytes in structures of the oral cavity are scarce. Hence, the present morphologic study was undertaken to explore for the first time the presence and specific localization of Telocytes within tissues of the normal human tongue, a complex muscular organ whose main functions include taste, speech, and food manipulation in the oral cavity. Telocytes were initially identified by CD34 immunostaining and confirmed by CD34/PDGFRα double immunofluorescence and transmission electron microscopy. CD34+/PDGFRα+ Telocytes were organized in interstitial meshworks either in the tongue lamina propria or in the underlying striated muscle. Lingual Telocytes were immunonegative for CD31, c-kit and α-SMA. Telopodes were finely distributed throughout the stromal space and concentrated beneath the lingual epithelium and around CD31+ vessels, skeletal muscle bundles/fibers, and intramuscular nerves and ganglia. They also enveloped salivary gland units outside the α-SMA+ myoepithelial cells and delimited lymphoid aggregates. These findings establish Telocytes as a previously overlooked interstitial cell population worth investigating further in the setting of human tongue pathophysiology
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reappraising the microscopic anatomy of human testis identification of Telocyte networks in the peritubular and intertubular stromal space
Scientific Reports, 2018Co-Authors: Mirca Marini, Irene Rosa, Daniele Guasti, Lidia Ibbamanneschi, Mauro Gacci, Eleonora Sgambati, Mirko ManettiAbstract:Telocytes are a recently described stromal cell type widely distributed in various organs including the female and male reproductive systems. This study was aimed to investigate for the first time the existence, distribution and characteristics of Telocytes in normal human testis by an integrated morphological approach (immunohistochemistry, immunofluorescence and transmission electron microscopy). We found that Telocytes displaying typical long and moniliform prolongations and coexpressing CD34 and PDGFRα formed networks in the outer layer of peritubular tissue and around Leydig cells and vessels in the intertubular stroma. Testicular Telocytes were immunophenotypically negative for CD31, c-kit/CD117 as well as α-SMA, thus making them clearly distinguishable from myoid cells/myofibroblasts located in the inner layer of peritubular tissue. Transmission electron microscopy confirmed the presence of cells ultrastructurally identifiable as Telocytes (i.e. cells with telopodes alternating podomers and podoms) in the aforementioned locations. Intercellular contacts between neighboring Telocytes and telopodes were observed throughout the testicular stromal compartment. Telopodes intimately surrounded and often established close contacts with peritubular myoid cells/myofibroblasts, Leydig cells and vessels. Extracellular vesicles were also frequently detected near telopodes. In summary, we demonstrated that Telocytes are a previously neglected stromal component of human testis with potential implications in tissue homeostasis deserving further investigation.
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Morphological evidence of Telocytes in human synovium
Nature Publishing Group, 2018Co-Authors: Irene Rosa, Lidia Ibba-manneschi, Mirca Marini, Daniele Guasti, Mirko ManettiAbstract:Abstract A new cell type named Telocyte (i.e. cell with distinctive prolongations called telopodes) has recently been identified in the stroma of various organs in humans. However, no study has yet reported the existence of Telocytes in the synovial membrane of diarthrodial joints. This work was therefore undertaken to search for Telocytes in the normal human synovium using transmission electron microscopy, immunohistochemistry and immunofluorescence. Ultrastructural analyses demonstrated the presence of numerous spindle-shaped Telocytes in the whole synovial sublining layer. Synovial Telocytes exhibited very long and thin moniliform telopodes and were particularly concentrated at the boundary between the lining and sublining layers and around blood vessels. Light microscopy confirmed the presence of CD34-positive Telocytes in the aforementioned locations. Moreover, synovial Telocytes coexpressed CD34 and platelet-derived growth factor receptor α. Double immunostaining further allowed to unequivocally differentiate synovial Telocytes (CD34-positive/CD31-negative) from vascular endothelial cells (CD34-positive/CD31-positive). The in vitro examination of fibroblast-like synoviocyte primary cultures revealed the coexistence of different cell types, including CD34-positive Telocytes projecting typical moniliform telopodes. In conclusion, our work provides the first evidence that Telocytes do exist in the human synovium and lays the groundwork for future studies on synovial Telocytes in a variety of degenerative and destructive joint diseases
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Telocytes in Chronic Inflammatory and Fibrotic Diseases.
Advances in experimental medicine and biology, 2016Co-Authors: Lidia Ibba-manneschi, Irene Rosa, Mirko ManettiAbstract:Telocytes are a peculiar stromal (interstitial) cell type implicated in tissue homeostasis and development, as well as in the pathophysiology of several disorders. Severe damage and reduction of Telocytes have been reported during fibrotic remodeling of multiple organs in various diseases, including scleroderma, Crohn’s disease, ulcerative colitis, and liver fibrosis, as well as in chronic inflammatory lesions like those of primary Sjogren’s syndrome and psoriasis. Owing to their close relationship with stem cells, Telocytes are also supposed to contribute to tissue repair/regeneration. Indeed, Telocytes are universally considered as “connecting cells” mostly oriented to intercellular signaling. On the basis of recent promising experimental findings, in the near future, Telocyte transplantation might represent a novel therapeutic opportunity to control the evolution of chronic inflammatory and fibrotic diseases. Notably, there is evidence to support that Telocytes could help in preventing abnormal activation of immune cells and fibroblasts, as well as in attenuating the altered matrix organization during the fibrotic process. By targeting Telocytes alone or in tandem with stem cells, we might be able to promote regeneration and prevent the evolution to irreversible tissue injury. Besides exogenous transplantation, exploring pharmacological or non-pharmacological methods to enhance the growth and/or survival of Telocytes could be an additional therapeutic strategy for many disorders.
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Telocyte implications in human pathology: An overview
Seminars in cell & developmental biology, 2016Co-Authors: Lidia Ibba-manneschi, Irene Rosa, Mirko ManettiAbstract:Telocytes are a recently described interstitial cell population widely distributed in the stromal compartment of many organs in vertebrates, including humans. Owing to their close spatial relationship with multiple cell types, Telocytes are universally considered as 'connecting cells' mostly committed to intercellular signaling by converting the interstitium into an integrated system that drives organ development and contributes to the maintenance of local tissue homeostasis. Increasing evidence indicates that Telocytes may cooperate with tissue-resident stem cells to foster organ repair and regeneration, and that Telocyte damage and dysfunction may occur in several disorders. The goal of this review is to provide an overview of the most recent findings concerning the implication of Telocytes in a variety of pathologic conditions in humans, including heart disease, chronic inflammation and multiorgan fibrosis. Based on recent promising experimental data, there is realistic hope that by targeting Telocytes alone or in tandem with stem cells, we might be able to promote organ regeneration and/or prevent irreversible end-stage organ damage in different pathologies.
Irene Rosa - One of the best experts on this subject based on the ideXlab platform.
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Telocytes constitute a widespread interstitial meshwork in the lamina propria and underlying striated muscle of human tongue
Nature Publishing Group, 2019Co-Authors: Irene Rosa, Lidia Ibba-manneschi, Mirca Marini, Cecilia Taverna, Luca Novelli, Mirko ManettiAbstract:Abstract Telocytes have recently emerged as unique interstitial cells defined by their extremely long, thin and moniliform prolongations termed telopodes. Despite growing evidence that these cells consistently reside in the stromal compartment of various organs from human beings, studies dealing with Telocytes in structures of the oral cavity are scarce. Hence, the present morphologic study was undertaken to explore for the first time the presence and specific localization of Telocytes within tissues of the normal human tongue, a complex muscular organ whose main functions include taste, speech, and food manipulation in the oral cavity. Telocytes were initially identified by CD34 immunostaining and confirmed by CD34/PDGFRα double immunofluorescence and transmission electron microscopy. CD34+/PDGFRα+ Telocytes were organized in interstitial meshworks either in the tongue lamina propria or in the underlying striated muscle. Lingual Telocytes were immunonegative for CD31, c-kit and α-SMA. Telopodes were finely distributed throughout the stromal space and concentrated beneath the lingual epithelium and around CD31+ vessels, skeletal muscle bundles/fibers, and intramuscular nerves and ganglia. They also enveloped salivary gland units outside the α-SMA+ myoepithelial cells and delimited lymphoid aggregates. These findings establish Telocytes as a previously overlooked interstitial cell population worth investigating further in the setting of human tongue pathophysiology
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reappraising the microscopic anatomy of human testis identification of Telocyte networks in the peritubular and intertubular stromal space
Scientific Reports, 2018Co-Authors: Mirca Marini, Irene Rosa, Daniele Guasti, Lidia Ibbamanneschi, Mauro Gacci, Eleonora Sgambati, Mirko ManettiAbstract:Telocytes are a recently described stromal cell type widely distributed in various organs including the female and male reproductive systems. This study was aimed to investigate for the first time the existence, distribution and characteristics of Telocytes in normal human testis by an integrated morphological approach (immunohistochemistry, immunofluorescence and transmission electron microscopy). We found that Telocytes displaying typical long and moniliform prolongations and coexpressing CD34 and PDGFRα formed networks in the outer layer of peritubular tissue and around Leydig cells and vessels in the intertubular stroma. Testicular Telocytes were immunophenotypically negative for CD31, c-kit/CD117 as well as α-SMA, thus making them clearly distinguishable from myoid cells/myofibroblasts located in the inner layer of peritubular tissue. Transmission electron microscopy confirmed the presence of cells ultrastructurally identifiable as Telocytes (i.e. cells with telopodes alternating podomers and podoms) in the aforementioned locations. Intercellular contacts between neighboring Telocytes and telopodes were observed throughout the testicular stromal compartment. Telopodes intimately surrounded and often established close contacts with peritubular myoid cells/myofibroblasts, Leydig cells and vessels. Extracellular vesicles were also frequently detected near telopodes. In summary, we demonstrated that Telocytes are a previously neglected stromal component of human testis with potential implications in tissue homeostasis deserving further investigation.
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Morphological evidence of Telocytes in human synovium
Nature Publishing Group, 2018Co-Authors: Irene Rosa, Lidia Ibba-manneschi, Mirca Marini, Daniele Guasti, Mirko ManettiAbstract:Abstract A new cell type named Telocyte (i.e. cell with distinctive prolongations called telopodes) has recently been identified in the stroma of various organs in humans. However, no study has yet reported the existence of Telocytes in the synovial membrane of diarthrodial joints. This work was therefore undertaken to search for Telocytes in the normal human synovium using transmission electron microscopy, immunohistochemistry and immunofluorescence. Ultrastructural analyses demonstrated the presence of numerous spindle-shaped Telocytes in the whole synovial sublining layer. Synovial Telocytes exhibited very long and thin moniliform telopodes and were particularly concentrated at the boundary between the lining and sublining layers and around blood vessels. Light microscopy confirmed the presence of CD34-positive Telocytes in the aforementioned locations. Moreover, synovial Telocytes coexpressed CD34 and platelet-derived growth factor receptor α. Double immunostaining further allowed to unequivocally differentiate synovial Telocytes (CD34-positive/CD31-negative) from vascular endothelial cells (CD34-positive/CD31-positive). The in vitro examination of fibroblast-like synoviocyte primary cultures revealed the coexistence of different cell types, including CD34-positive Telocytes projecting typical moniliform telopodes. In conclusion, our work provides the first evidence that Telocytes do exist in the human synovium and lays the groundwork for future studies on synovial Telocytes in a variety of degenerative and destructive joint diseases
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Telocytes in Chronic Inflammatory and Fibrotic Diseases.
Advances in experimental medicine and biology, 2016Co-Authors: Lidia Ibba-manneschi, Irene Rosa, Mirko ManettiAbstract:Telocytes are a peculiar stromal (interstitial) cell type implicated in tissue homeostasis and development, as well as in the pathophysiology of several disorders. Severe damage and reduction of Telocytes have been reported during fibrotic remodeling of multiple organs in various diseases, including scleroderma, Crohn’s disease, ulcerative colitis, and liver fibrosis, as well as in chronic inflammatory lesions like those of primary Sjogren’s syndrome and psoriasis. Owing to their close relationship with stem cells, Telocytes are also supposed to contribute to tissue repair/regeneration. Indeed, Telocytes are universally considered as “connecting cells” mostly oriented to intercellular signaling. On the basis of recent promising experimental findings, in the near future, Telocyte transplantation might represent a novel therapeutic opportunity to control the evolution of chronic inflammatory and fibrotic diseases. Notably, there is evidence to support that Telocytes could help in preventing abnormal activation of immune cells and fibroblasts, as well as in attenuating the altered matrix organization during the fibrotic process. By targeting Telocytes alone or in tandem with stem cells, we might be able to promote regeneration and prevent the evolution to irreversible tissue injury. Besides exogenous transplantation, exploring pharmacological or non-pharmacological methods to enhance the growth and/or survival of Telocytes could be an additional therapeutic strategy for many disorders.
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Telocyte implications in human pathology: An overview
Seminars in cell & developmental biology, 2016Co-Authors: Lidia Ibba-manneschi, Irene Rosa, Mirko ManettiAbstract:Telocytes are a recently described interstitial cell population widely distributed in the stromal compartment of many organs in vertebrates, including humans. Owing to their close spatial relationship with multiple cell types, Telocytes are universally considered as 'connecting cells' mostly committed to intercellular signaling by converting the interstitium into an integrated system that drives organ development and contributes to the maintenance of local tissue homeostasis. Increasing evidence indicates that Telocytes may cooperate with tissue-resident stem cells to foster organ repair and regeneration, and that Telocyte damage and dysfunction may occur in several disorders. The goal of this review is to provide an overview of the most recent findings concerning the implication of Telocytes in a variety of pathologic conditions in humans, including heart disease, chronic inflammation and multiorgan fibrosis. Based on recent promising experimental data, there is realistic hope that by targeting Telocytes alone or in tandem with stem cells, we might be able to promote organ regeneration and/or prevent irreversible end-stage organ damage in different pathologies.
Lidia Ibba-manneschi - One of the best experts on this subject based on the ideXlab platform.
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Telocytes constitute a widespread interstitial meshwork in the lamina propria and underlying striated muscle of human tongue
Nature Publishing Group, 2019Co-Authors: Irene Rosa, Lidia Ibba-manneschi, Mirca Marini, Cecilia Taverna, Luca Novelli, Mirko ManettiAbstract:Abstract Telocytes have recently emerged as unique interstitial cells defined by their extremely long, thin and moniliform prolongations termed telopodes. Despite growing evidence that these cells consistently reside in the stromal compartment of various organs from human beings, studies dealing with Telocytes in structures of the oral cavity are scarce. Hence, the present morphologic study was undertaken to explore for the first time the presence and specific localization of Telocytes within tissues of the normal human tongue, a complex muscular organ whose main functions include taste, speech, and food manipulation in the oral cavity. Telocytes were initially identified by CD34 immunostaining and confirmed by CD34/PDGFRα double immunofluorescence and transmission electron microscopy. CD34+/PDGFRα+ Telocytes were organized in interstitial meshworks either in the tongue lamina propria or in the underlying striated muscle. Lingual Telocytes were immunonegative for CD31, c-kit and α-SMA. Telopodes were finely distributed throughout the stromal space and concentrated beneath the lingual epithelium and around CD31+ vessels, skeletal muscle bundles/fibers, and intramuscular nerves and ganglia. They also enveloped salivary gland units outside the α-SMA+ myoepithelial cells and delimited lymphoid aggregates. These findings establish Telocytes as a previously overlooked interstitial cell population worth investigating further in the setting of human tongue pathophysiology
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Morphological evidence of Telocytes in human synovium
Nature Publishing Group, 2018Co-Authors: Irene Rosa, Lidia Ibba-manneschi, Mirca Marini, Daniele Guasti, Mirko ManettiAbstract:Abstract A new cell type named Telocyte (i.e. cell with distinctive prolongations called telopodes) has recently been identified in the stroma of various organs in humans. However, no study has yet reported the existence of Telocytes in the synovial membrane of diarthrodial joints. This work was therefore undertaken to search for Telocytes in the normal human synovium using transmission electron microscopy, immunohistochemistry and immunofluorescence. Ultrastructural analyses demonstrated the presence of numerous spindle-shaped Telocytes in the whole synovial sublining layer. Synovial Telocytes exhibited very long and thin moniliform telopodes and were particularly concentrated at the boundary between the lining and sublining layers and around blood vessels. Light microscopy confirmed the presence of CD34-positive Telocytes in the aforementioned locations. Moreover, synovial Telocytes coexpressed CD34 and platelet-derived growth factor receptor α. Double immunostaining further allowed to unequivocally differentiate synovial Telocytes (CD34-positive/CD31-negative) from vascular endothelial cells (CD34-positive/CD31-positive). The in vitro examination of fibroblast-like synoviocyte primary cultures revealed the coexistence of different cell types, including CD34-positive Telocytes projecting typical moniliform telopodes. In conclusion, our work provides the first evidence that Telocytes do exist in the human synovium and lays the groundwork for future studies on synovial Telocytes in a variety of degenerative and destructive joint diseases
-
Telocytes in Chronic Inflammatory and Fibrotic Diseases.
Advances in experimental medicine and biology, 2016Co-Authors: Lidia Ibba-manneschi, Irene Rosa, Mirko ManettiAbstract:Telocytes are a peculiar stromal (interstitial) cell type implicated in tissue homeostasis and development, as well as in the pathophysiology of several disorders. Severe damage and reduction of Telocytes have been reported during fibrotic remodeling of multiple organs in various diseases, including scleroderma, Crohn’s disease, ulcerative colitis, and liver fibrosis, as well as in chronic inflammatory lesions like those of primary Sjogren’s syndrome and psoriasis. Owing to their close relationship with stem cells, Telocytes are also supposed to contribute to tissue repair/regeneration. Indeed, Telocytes are universally considered as “connecting cells” mostly oriented to intercellular signaling. On the basis of recent promising experimental findings, in the near future, Telocyte transplantation might represent a novel therapeutic opportunity to control the evolution of chronic inflammatory and fibrotic diseases. Notably, there is evidence to support that Telocytes could help in preventing abnormal activation of immune cells and fibroblasts, as well as in attenuating the altered matrix organization during the fibrotic process. By targeting Telocytes alone or in tandem with stem cells, we might be able to promote regeneration and prevent the evolution to irreversible tissue injury. Besides exogenous transplantation, exploring pharmacological or non-pharmacological methods to enhance the growth and/or survival of Telocytes could be an additional therapeutic strategy for many disorders.
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Telocyte implications in human pathology: An overview
Seminars in cell & developmental biology, 2016Co-Authors: Lidia Ibba-manneschi, Irene Rosa, Mirko ManettiAbstract:Telocytes are a recently described interstitial cell population widely distributed in the stromal compartment of many organs in vertebrates, including humans. Owing to their close spatial relationship with multiple cell types, Telocytes are universally considered as 'connecting cells' mostly committed to intercellular signaling by converting the interstitium into an integrated system that drives organ development and contributes to the maintenance of local tissue homeostasis. Increasing evidence indicates that Telocytes may cooperate with tissue-resident stem cells to foster organ repair and regeneration, and that Telocyte damage and dysfunction may occur in several disorders. The goal of this review is to provide an overview of the most recent findings concerning the implication of Telocytes in a variety of pathologic conditions in humans, including heart disease, chronic inflammation and multiorgan fibrosis. Based on recent promising experimental data, there is realistic hope that by targeting Telocytes alone or in tandem with stem cells, we might be able to promote organ regeneration and/or prevent irreversible end-stage organ damage in different pathologies.
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Telocytes in minor salivary glands of primary Sjögren's syndrome: association with the extent of inflammation and ectopic lymphoid neogenesis
Journal of cellular and molecular medicine, 2015Co-Authors: Alessia Alunno, Lidia Ibba-manneschi, Irene Rosa, Onelia Bistoni, Sara Caterbi, Roberto Gerli, Mirko ManettiAbstract:It has been recently reported that Telocytes, a stromal (interstitial) cell subset involved in the control of local tissue homeostasis, are hampered in the target organs of inflammatory/autoimmune disorders. Since no data concerning Telocytes in minor salivary glands (MSGs) are currently available, aim of the study was to evaluate Telocyte distribution in MSGs with normal architecture, non-specific chronic sialadenitis (NSCS) and primary Sjogren's syndrome (pSS)-focal lymphocytic sialadenitis. Twelve patients with pSS and 16 sicca non-pSS subjects were enrolled in the study. MSGs were evaluated by haematoxylin and eosin staining and immunofluorescence for CD3/CD20 and CD21 to assess focus score, Tarpley biopsy score, T/B cell segregation and germinal center (GC)-like structures. Telocytes were identified by immunoperoxidase-based immunohistochemistry for CD34 and CD34/platelet-derived growth factor receptor α double immunofluorescence. Telocytes were numerous in the stromal compartment of normal MSGs, where their long cytoplasmic processes surrounded vessels and encircled both the excretory ducts and the secretory units. In NSCS, despite the presence of a certain degree of inflammation, Telocytes were normally represented. Conversely, Telocytes were markedly reduced in MSGs from pSS patients compared to normal and NSCS MSGs. Such a decrease was associated with both worsening of glandular inflammation and progression of ectopic lymphoid neogenesis, periductal Telocytes being reduced in the presence of smaller inflammatory foci and completely absent in the presence of GC-like structures. Our findings suggest that a loss of MSG Telocytes might have important pathophysiological implications in pSS. The specific pro-inflammatory cytokine milieu of pSS MSGs might be one of the causes of Telocyte loss.
Xiangdong Wang - One of the best experts on this subject based on the ideXlab platform.
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Influence of gene modification in biological behaviors and responses of mouse lung Telocytes to inflammation.
Journal of translational medicine, 2019Co-Authors: Dongli Song, Yile Zhou, Hao Fang, Xiangdong WangAbstract:Telocytes play key roles in maintenance of organ/tissue function and prevention of organ injury. However, there are great challenges to investigate Telocytes functions using primary Telocytes, due to the difficulties of isolation, identification, and stability. The present study aims at constructing continuous cell strain of mouse lung Telocyte cell line with stable characters by gene modification and investigating biological behaviors and responses of gene-modified Telocytes to inflammation. Mouse primary lung Telocytes were isolated and identified using immune-labeling markers and immunoelectron microscopy. Primary Telocytes were transformed with Simian vacuolating virus 40 small and large T antigen (SV40). Biological characters, behaviors morphology, and proliferation of those gene-modified Telocytes were defined and monitored dynamically for 50 generations, as compared with primary lung Telocytes. Cell cycle of mouse primary lung Telocytes or gene-modified Telocytes was detected by flow cytometry. Gene modified Telocytes of generations 5, 10, 30 and 50 were observed with telopodes and also showed CD34 and ckit positive. Multiple cellular morphology were also observed on Telocyte cell-line under monitor of celliq and enhanced cell proliferation were showed. SV40 transduction was also reduced apoptosis and increased the ratio of S and G2 phases in Telocyte cell-line. We successfully constructed mouse lung Telocyte cell-line which maintained the biological properties and behaviors as primary Telocytes and could responses to inflammation induced by LPS. Thus, gene-modified lung Telocytes, Telocyte Line, would provide a cell tool for researchers exploring the roles and applications of Telocytes involved in physiological and pathological states in future.
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The History of Telocyte Discovery and Understanding.
Advances in experimental medicine and biology, 2016Co-Authors: Jian Wang, Meiling Jin, Zhitu Zhu, Xiangdong WangAbstract:Telocytes (TCs) are identified as a peculiar cell type of interstitial cells in various organs. The typical features of TCs from the other cells are the extending cellular process as telopodes with alternation of podomeres and podoms. Before the year of 2010, TCs were considered as interstitial Cajal-like cells because of the similar morphology and immunohistochemical features with interstitial cells of Cajal which were found more than 100 years ago and considered to be pacemakers for gut motility. Subsequently, it demonstrated that TCs were not Cajal-like cells, and thus the new name “Telocyte” was proposed in 2010. With the help of different techniques, e.g., transmission electron microscopy, immunohistochemistry, or omics science, TCs have been detected in various tissues and organs from different species. The pathological role of TCs in different diseases was also studied. According to observation in situ or in vitro, TCs played a vital role in mechanical support, signaling transduction, tissue renewal or repair, immune surveillance, and mechanical sensor via establishing homo- or heterogenous junctions with neighboring cells to form 3D network or release extracellular vesicles to form juxtacrine and paracrine. This review will introduce the origin, distribution, morphology, functions, omics science, methods, and interaction of TCs with other cells and provide a better understanding of the new cell type.
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Roles of Telocytes in the Development of Angiogenesis.
Advances in experimental medicine and biology, 2016Co-Authors: Yonghua Zheng, Xiangdong WangAbstract:Telocytes are cells with telopodes, which distinguish them from other interstitial cells. According to the study of lung, it was confirmed that Telocytes were mainly distributed in the alveolar interstitial tissues connected tightly with alveolar epithelia cells and participated in the structure of air-blood barrier, in the small vein and bronchioles and in the interstitial space of smooth muscle participated in the frame structure of the blood and bronchioles. Telocytes are positive to CD34 and C-kit which expressed on the surface of hemopoietic stem cells, and are proposed to participate in the angiogenesis. In this chapter, we try to clarify the morphological characteristics of lung Telocytes, both in tissues and culture, and introduce the experiences on the method of Telocytes isolation and primary culture. The proteomics analysis of lung Telocytes through iTRAQ (isobaric tags for relative and absolute quantification) was also discussed and it will provide new research directions in the future.
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Human lung Telocytes could promote the proliferation and angiogenesis of human pulmonary microvascular endothelial cells in vitro
Molecular and cellular therapies, 2014Co-Authors: Yonghua Zheng, Chunxue Bai, Xiaoke Chen, Mengjia Qian, Miaomiao Zhang, Ding Zhang, Qun Wang, Xiangdong WangAbstract:In the previous studies, Telocytes were found near the capillaries in many tissues, especially on the extracellular matrix of blood vessels and positive to CD34 and c-kit. Therefore, the present study aimed to explore if Telocytes could produce angiogenesis associated cytokines, promote the proliferation and the angiogenesis of vascular endothelial cells in vitro. Human lung Telocytes were isolated and cultured, and were identified by immunofluorescence cytochemistry with CD34, c-kit and vimentin. Telocytes conditional media (TCM) was prepared, and the expressions of angiogenesis associated cytokines in TCM were detected by ELISA. Human pulmonary microvascular endothelial cells (HPMECs) were cultured with DMEM media or TCM for 72 hours. The proliferation of HPMECs was continuously detected with CCK-8 kit at an interval of 12 hours. HPMECs were also injured by lipopolysaccharide, and cultured with TCM and DMEM respectively, and the tube formation capacity was detected. Telocytes were positive for CD34, c-kit and vimentin. The expressions of VEGF and EGF in TCM were significantly higher, the proliferation of HPMECs cultured with TCM significantly increased, and the tube formation of HPMECs injured by endotoxin was improved with the culture of TCM, as compared with the culture of DMEM. The present study provides the evidence that human lung Telocytes could produce the growth factors, such as VEGF and EGF. Telocytes conditional media induced the proliferation of pulmonary endothelial cells and prevented from endotoxin-induced compromise of pulmonary endothelial angiogenesis.
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Telocytes in the urinary system
Journal of translational medicine, 2012Co-Authors: Yonghua Zheng, Tongyu Zhu, Miao Lin, Xiangdong WangAbstract:Background Telocytes, a new type of interstitial cells, have been identified in many organs in mammals. The present studies aimed at investigating the ultrastructure, distribution and interactions of Telocytes with surrounding cells in the urinary system of rats, to confirm the existence of Telocytes in kidneys, ureter and urinary bladder.
Klaus H. Kaestner - One of the best experts on this subject based on the ideXlab platform.
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Emerging diverse roles of Telocytes.
Development (Cambridge England), 2019Co-Authors: Ayano Kondo, Klaus H. KaestnerAbstract:Since the first description of 'interstitial cells of Cajal' in the mammalian gut in 1911, scientists have found structurally similar cells, now termed Telocytes, in numerous tissues throughout the body. These cells have recently sparked renewed interest, facilitated through the development of a molecular handle to genetically manipulate their function in tissue homeostasis and disease. In this Primer, we discuss the discovery of Telocytes, their physical properties, distribution and function, focusing on recent developments in the functional analysis of Foxl1-positive Telocytes in the intestinal stem cell niche, and, finally, the current challenges of studying Telocytes as a distinct cell type.
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Subepithelial Telocytes are an important source of Wnts that supports intestinal crypts
Nature, 2018Co-Authors: Michal Shoshkes-carmel, Shalev Itzkovitz, Yue J. Wang, Kirk J. Wangensteen, Beáta Tóth, Ayano Kondo, Efi E. Massasa, Klaus H. KaestnerAbstract:Tissues that undergo rapid cellular turnover, such as the mammalian haematopoietic system or the intestinal epithelium, are dependent on stem and progenitor cells that proliferate to provide differentiated cells to maintain organismal health. Stem and progenitor cells, in turn, are thought to rely on signals and growth factors provided by local niche cells to support their function and self-renewal. Several cell types have been hypothesized to provide the signals required for the proliferation and differentiation of the intestinal stem cells in intestinal crypts^ 1 – 6 . Here we identify subepithelial Telocytes as an important source of Wnt proteins, without which intestinal stem cells cannot proliferate and support epithelial renewal. Telocytes are large but rare mesenchymal cells that are marked by expression of FOXL1 and form a subepithelial plexus that extends from the stomach to the colon. While supporting the entire epithelium, FOXL1^+ Telocytes compartmentalize the production of Wnt ligands and inhibitors to enable localized pathway activation. Conditional genetic ablation of porcupine ( Porcn ), which is required for functional maturation of all Wnt proteins, in mouse FOXL1^+ Telocytes causes rapid cessation of Wnt signalling to intestinal crypts, followed by loss of proliferation of stem and transit amplifying cells and impaired epithelial renewal. Thus, FOXL1^+ Telocytes are an important source of niche signals to intestinal stem cells. Subepithelial Telocytes are identified as a source of Wnt signals that enable proliferation and differentiation of intestinal stem cells, an essential function for maintenance of the intestinal epithelium.
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Subepithelial Telocytes are an important source of Wnts that supports intestinal crypts
Nature, 2018Co-Authors: Michal Shoshkes-carmel, Shalev Itzkovitz, Yue J. Wang, Kirk J. Wangensteen, Beáta Tóth, Ayano Kondo, Efi E. Massasa, Klaus H. KaestnerAbstract:Tissues that undergo rapid cellular turnover, such as the mammalian haematopoietic system or the intestinal epithelium, are dependent on stem and progenitor cells that proliferate to provide differentiated cells to maintain organismal health. Stem and progenitor cells, in turn, are thought to rely on signals and growth factors provided by local niche cells to support their function and self-renewal. Several cell types have been hypothesized to provide the signals required for the proliferation and differentiation of the intestinal stem cells in intestinal crypts1-6. Here we identify subepithelial Telocytes as an important source of Wnt proteins, without which intestinal stem cells cannot proliferate and support epithelial renewal. Telocytes are large but rare mesenchymal cells that are marked by expression of FOXL1 and form a subepithelial plexus that extends from the stomach to the colon. While supporting the entire epithelium, FOXL1+ Telocytes compartmentalize the production of Wnt ligands and inhibitors to enable localized pathway activation. Conditional genetic ablation of porcupine (Porcn), which is required for functional maturation of all Wnt proteins, in mouse FOXL1+ Telocytes causes rapid cessation of Wnt signalling to intestinal crypts, followed by loss of proliferation of stem and transit amplifying cells and impaired epithelial renewal. Thus, FOXL1+ Telocytes are an important source of niche signals to intestinal stem cells.
