The Experts below are selected from a list of 264 Experts worldwide ranked by ideXlab platform
Zhaohui Li - One of the best experts on this subject based on the ideXlab platform.
-
A fluorescent nanosphere-based immunochromatography test strip for ultrasensitive and point-of-care detection of Tetanus Antibody in human serum.
Analytical and Bioanalytical Chemistry, 2019Co-Authors: Juan Chen, Hongmin Meng, Lingbo Qu, Ying An, Ran Yang, Zhaohui LiAbstract:Tetanus still possesses a high infection risk and leads a number of human deaths in poor nations. Point-of-care and ultrasensitive detection of Tetanus Antibody levels in serum is the key to decrease the risk of Tetanus infection and improve the health of people. In this work, by using ultra bright fluorescent nanospheres (FNs) and portable lateral flow test strip (LFTS), a point-of-care and ultrasensitive sensing method has been developed for the detection of Tetanus antibodies in human serum. This assay works quite well for Tetanus antibodies in the concentration range from 0.0002 to 0.0220 IU/mL with a low detection limit of 0.00011 IU/mL, which is 100-fold lower than conventional gold-based LFTSs. The high sensitivity makes this method suitable for use to detect the low-abundant target in real samples. Besides, this cost-effective FN-based LFTS assay possesses good selectivity, high accuracy, and satisfactory reliability, which holds great potential as a robust candidate for routine medical diagnosis and rapid home testing.
-
quantum dot based lateral flow test strips for highly sensitive detection of the Tetanus Antibody
ACS Omega, 2019Co-Authors: Junyan Wang, Hongmin Meng, Juan Chen, Lin Zhang, Lingbo Qu, Zhaohui LiAbstract:Tetanus possesses a high infection rate and remains a leading cause of infection in some developing countries. To reduce Tetanus infection, point-of-care (POC) monitoring of the serum Tetanus Antibody level is highly desired. Here, we report fluorescent lateral flow test strips (LFTS) for simple, sensitive, and rapid detection of the Tetanus Antibody. By using a sandwich immunoreaction, LFTS are easily prepared. In the existence of the Tetanus Antibody, goat anti-human IgG (Fc)-labeled quantum dots (QDs) are captured on the test line via the formation of a sandwich structure complex of QD-labeled goat anti-human IgG (Fc)–Tetanus Antibody–Tetanus antigens. The assay can be completed in 30 min. These LFTS possess high sensitivity for Tetanus Antibody detection, with a detection limit of 0.001 IU mL–1, which is 10 times lower than that of the Au nanoparticle-based test strip. The developed sensing system was also successfully applied for detection of the Tetanus Antibody in the human serum. Moreover, these s...
-
a lateral flow assay for the determination of human Tetanus Antibody in whole blood by using gold nanoparticle labeled Tetanus antigen
Mikrochimica Acta, 2018Co-Authors: Junyan Wang, Hongmin Meng, Lin Zhang, Zhaohui Li, Hongqi Wang, Jianjun Li, Lingbo QuAbstract:The authors describe a lateral flow assay (LFA) for the Antibody against the infectious bacterium Clostridium tetani. Gold nanoparticles (AuNPs) were linked to Tetanus antigen and are captured in the test line via the formation of a sandwich structure composed of AuNP-labeled Tetanus antigen, Tetanus Antibody, and Tetanus antigen. This leads to the formation of a characteristic red line due to the accumulation of AuNPs. The formation of the color line allows for a highly sensitive and selective detection of Tetanus Antibody, both with bare eyes and by smartphone-based quantitative analysis. This assay offers a wide detection range from 0 to 0.5 IU·mL−1 and has a linear relationship from 0.01 to 0.1 IU·mL−1 with an experimental detection limit of 0.01 IU·mL−1. This assay is simple, fast, inexpensive and highly selective. When applied to the detection of Tetanus Antibody in spiked whole blood, it provided reliable results that compared well to those obtained with a commercial ELISA kit.
Lingbo Qu - One of the best experts on this subject based on the ideXlab platform.
-
A fluorescent nanosphere-based immunochromatography test strip for ultrasensitive and point-of-care detection of Tetanus Antibody in human serum.
Analytical and Bioanalytical Chemistry, 2019Co-Authors: Juan Chen, Hongmin Meng, Lingbo Qu, Ying An, Ran Yang, Zhaohui LiAbstract:Tetanus still possesses a high infection risk and leads a number of human deaths in poor nations. Point-of-care and ultrasensitive detection of Tetanus Antibody levels in serum is the key to decrease the risk of Tetanus infection and improve the health of people. In this work, by using ultra bright fluorescent nanospheres (FNs) and portable lateral flow test strip (LFTS), a point-of-care and ultrasensitive sensing method has been developed for the detection of Tetanus antibodies in human serum. This assay works quite well for Tetanus antibodies in the concentration range from 0.0002 to 0.0220 IU/mL with a low detection limit of 0.00011 IU/mL, which is 100-fold lower than conventional gold-based LFTSs. The high sensitivity makes this method suitable for use to detect the low-abundant target in real samples. Besides, this cost-effective FN-based LFTS assay possesses good selectivity, high accuracy, and satisfactory reliability, which holds great potential as a robust candidate for routine medical diagnosis and rapid home testing.
-
quantum dot based lateral flow test strips for highly sensitive detection of the Tetanus Antibody
ACS Omega, 2019Co-Authors: Junyan Wang, Hongmin Meng, Juan Chen, Lin Zhang, Lingbo Qu, Zhaohui LiAbstract:Tetanus possesses a high infection rate and remains a leading cause of infection in some developing countries. To reduce Tetanus infection, point-of-care (POC) monitoring of the serum Tetanus Antibody level is highly desired. Here, we report fluorescent lateral flow test strips (LFTS) for simple, sensitive, and rapid detection of the Tetanus Antibody. By using a sandwich immunoreaction, LFTS are easily prepared. In the existence of the Tetanus Antibody, goat anti-human IgG (Fc)-labeled quantum dots (QDs) are captured on the test line via the formation of a sandwich structure complex of QD-labeled goat anti-human IgG (Fc)–Tetanus Antibody–Tetanus antigens. The assay can be completed in 30 min. These LFTS possess high sensitivity for Tetanus Antibody detection, with a detection limit of 0.001 IU mL–1, which is 10 times lower than that of the Au nanoparticle-based test strip. The developed sensing system was also successfully applied for detection of the Tetanus Antibody in the human serum. Moreover, these s...
-
a lateral flow assay for the determination of human Tetanus Antibody in whole blood by using gold nanoparticle labeled Tetanus antigen
Mikrochimica Acta, 2018Co-Authors: Junyan Wang, Hongmin Meng, Lin Zhang, Zhaohui Li, Hongqi Wang, Jianjun Li, Lingbo QuAbstract:The authors describe a lateral flow assay (LFA) for the Antibody against the infectious bacterium Clostridium tetani. Gold nanoparticles (AuNPs) were linked to Tetanus antigen and are captured in the test line via the formation of a sandwich structure composed of AuNP-labeled Tetanus antigen, Tetanus Antibody, and Tetanus antigen. This leads to the formation of a characteristic red line due to the accumulation of AuNPs. The formation of the color line allows for a highly sensitive and selective detection of Tetanus Antibody, both with bare eyes and by smartphone-based quantitative analysis. This assay offers a wide detection range from 0 to 0.5 IU·mL−1 and has a linear relationship from 0.01 to 0.1 IU·mL−1 with an experimental detection limit of 0.01 IU·mL−1. This assay is simple, fast, inexpensive and highly selective. When applied to the detection of Tetanus Antibody in spiked whole blood, it provided reliable results that compared well to those obtained with a commercial ELISA kit.
Kazım Karaarslan - One of the best experts on this subject based on the ideXlab platform.
-
Tetanus immunity in nursing home residents of Bolu, Turkey
BMC Public Health, 2005Co-Authors: Oguz Karabay, Ali Tamer, Fatma Ozkardes, Kazım KaraarslanAbstract:Background Tetanus is a serious but vaccine-preventable disease and fatality rate of the disease is high in the neonates and the elderly. The aim of this study was to detect the Tetanus Antibody prevalence in the over sixty-year age residents of the nursing homes in Bolu. Methods A voluntary-based study was done in the residents of two nursing homes in Bolu, Turkey. Blood samples were taken from 71 volunteers residing in there nursing homes. Tetanus IgG antibodies were measured by a commercial ELISA kit. Results Among overall subjects, only 11 (15.7 %) had the protective Tetanus Antibody titers at the time of the study. Totally, 10 subjects were examined in emergency rooms due to trauma or accidents within the last ten years and, four (40%) of them had protective Antibody levels. Of the remaining 61 subjects only 7 (11%) had protective Antibody levels (p < 0.05) [Relative Risk = 3.49, 95% Confidence Interval 1.24–9.77]. Conclusions Tetanus Antibody level is below the protective level in the majority of the over-sixty-year-age subjects residing in the nursing homes. Each over sixty-year age person in our country should be vaccinated. Until this is accomplished, at least, nursing home residents should be vaccinated during registration.
-
Tetanus immunity in nursing home residents of Bolu, Turkey
BMC Public Health, 2005Co-Authors: Oguz Karabay, Ali Tamer, Fatma Ozkardes, Kazım KaraarslanAbstract:Background Tetanus is a serious but vaccine-preventable disease and fatality rate of the disease is high in the neonates and the elderly. The aim of this study was to detect the Tetanus Antibody prevalence in the over sixty-year age residents of the nursing homes in Bolu.
Scott A Halperin - One of the best experts on this subject based on the ideXlab platform.
-
tolerability and Antibody response in adolescents and adults revaccinated with Tetanus toxoid reduced diphtheria toxoid and acellular pertussis vaccine adsorbed tdap 4 5 years after a previous dose
Vaccine, 2011Co-Authors: Scott A Halperin, Shelly A Mcneil, Joanne M Langley, Mark M Blatter, Marc Dionne, Joanne Embree, Roehl Johnson, Thomas Latiolais, William Meekison, Francisco NoyaAbstract:BACKGROUND: Although decennial adult boosters of Tetanus and diphtheria toxoids are recommended in Canada and the United States, a second dose of pertussis vaccine is not currently recommended for adults. METHODS: This open-label, postmarketing, multicenter study evaluated the tolerability and immunogenicity of a second dose of an adult formulation of Tetanus, diphtheria, and pertussis vaccine (Tdap) in adolescents and adults 5 years after a first dose. RESULTS: A total of 545 participants from previous Tdap vaccine studies, ranging in age from 15 to 69 years, participated in this study. Of these participants, 94.2% had at least one solicited adverse event after the booster dose such as injection-site erythema (28.6%), swelling (25.6%), or pain (87.6%) or a systemic adverse event such as myalgia (61.0%), headache (53.2%), malaise (38.2%), or fever (6.5%). These adverse events were slightly more frequent than after the initial dose. Postvaccination, 100% of participants had a Tetanus Antibody level ≥0.10IU/mL and 95% had a diphtheria Antibody level ≥0.10IU/mL. For pertussis, 82.1% (pertussis toxoid), 96.7% (filamentous hemagglutinin), 95.6% (pertactin), and 99.8% (fimbriae) had a postvaccination Antibody threshold of ≥50EU/mL. CONCLUSION: A second dose of Tdap vaccine 5 years after the initial dose was well tolerated and immunogenic in adolescents and adults.
-
kinetics of the Antibody response to Tetanus diphtheria acellular pertussis vaccine in women of childbearing age and postpartum women
Clinical Infectious Diseases, 2011Co-Authors: Beth A Halperin, Shelly A Mcneil, Joanne M Langley, A Morris, Donna Mackinnoncameron, Jill Mutch, Donna Macdougall, Scott A HalperinAbstract:Background. Because adolescents and adults act as a primary source of pertussis infection for infants, vaccination of mothers immediately postpartum is a potential strategy to reduce transmission (cocoon strategy). For this to be effective, high levels of antibodies must be achieved rapidly after vaccination. We sought to determine whether the Antibody response to Tetanus-diphtheria-acellular pertussis vaccine (Tdap) is sufficiently rapid to support the cocoon strategy. Methods. Two sequential studies were performed. The first was a nonrandomized, open study of a 5-pertussiscomponent Tdap vaccine (Tetanus toxoid, diphtheria toxoid, pertussis toxoid [PT], filamentous hemagglutinin [FHA], fimbriae types 2 and 3 [FIM], and pertactin [PRN]) given to women of childbearing age; the second was a randomized, open study of Tdap or no vaccine in postpartum women. Serum levels of immunoglobin (Ig) G and IgA against pertussis antigens, serum levels of IgG against diphtheria and Tetanus, and breast milk levels of IgA against pertussis antigens were measured at various times after vaccination. Results. In both studies, the Antibody response was relatively rapid, with serum IgG and IgA levels beginning to increase noticeably by days 5‐7 and approaching peak levels by day 14. Greater than 68% and 84.4% of IgG and IgA responders, respectively, achieved $90% of their maximum titer by day 14. The diphtheria and Tetanus Antibody kinetics followed a similar time course. Breast milk levels of IgA against PT, FHA, and FIM were first detectable at day 7, peaked by day 10, and then slowly decreased through day 28. Antibodies against PRN showed a similar response, although the peak occurred at day 14. There were no significant Antibody responses in the control group. Conclusions. Although the Antibody response to a dose of Tdap in healthy nonpregnant women of childbearing age and postpartum women occurs by day 14 and is suggestive of an anamnestic immune response, it may not be sufficiently rapid to protect infants in the first weeks of life.
Felicity T Cutts - One of the best experts on this subject based on the ideXlab platform.
-
maternal hiv infection and placental malaria reduce transplacental Antibody transfer and Tetanus Antibody levels in newborns in kenya
The Journal of Infectious Diseases, 2007Co-Authors: Phillippa M Cumberland, Caroline Shulman, P Chris A Maple, J N Bulmer, Edgar K Dorman, Ken Kawuondo, Kevin Marsh, Felicity T CuttsAbstract:Background. In clinical trials, maternal Tetanus toxoid (TT) vaccination is effective in protecting newborns against Tetanus infection, but inadequate placental transfer of Tetanus antibodies may contribute to lower-than-expected rates of protection in routine practice. We studied the effect of placental malaria and maternal human immunodeficiency virus (HIV) infection on placental transfer of antibodies to Tetanus.Methods. A total of 704 maternal-cord paired serum samples were tested by ELISA for antibodies to Tetanus. The HIV status of all women was determined by an immunoglobulin G Antibody-capture particle-adherence test, and placental malaria was determined by placental biopsy. Maternal history of TT vaccination was recorded.Results. Tetanus Antibody levels were reduced by 52% (95% confidence interval [CI], 30%-67%) in newborns of HIV-infected women and by 48% (95% CI, 26%-62%) in newborns whose mothers had active-chronic or past placental malaria. Thirty-seven mothers (5.3%) and 55 newborns (7.8%) had Tetanus Antibody levels <0.1 IU/mL (i.e., were seronegative). Mothers' self-reported history of lack of Tetanus immunization was the strongest predictor of seronegativity and of Tetanus Antibody levels in maternal and cord serum.Conclusion. Malarial and HIV infections may hinder efforts to eliminate maternal and neonatal Tetanus, making implementation of the current policy for mass vaccination of women of childbearing age an urgent priority.
-
Maternal HIV Infection and Placental Malaria Reduce Transplacental Antibody Transfer and Tetanus Antibody Levels in Newborns in Kenya
The Journal of Infectious Diseases, 2007Co-Authors: Phillippa M Cumberland, Caroline Shulman, P Chris A Maple, J N Bulmer, Edgar K Dorman, Ken Kawuondo, Kevin Marsh, Felicity T CuttsAbstract:Background. In clinical trials, maternal Tetanus toxoid (TT) vaccination is effective in protecting newborns against Tetanus infection, but inadequate placental transfer of Tetanus antibodies may contribute to lower-than-expected rates of protection in routine practice. We studied the effect of placental malaria and maternal human immunodeficiency virus (HIV) infection on placental transfer of antibodies to Tetanus.Methods. A total of 704 maternal-cord paired serum samples were tested by ELISA for antibodies to Tetanus. The HIV status of all women was determined by an immunoglobulin G Antibody-capture particle-adherence test, and placental malaria was determined by placental biopsy. Maternal history of TT vaccination was recorded.Results. Tetanus Antibody levels were reduced by 52% (95% confidence interval [CI], 30%-67%) in newborns of HIV-infected women and by 48% (95% CI, 26%-62%) in newborns whose mothers had active-chronic or past placental malaria. Thirty-seven mothers (5.3%) and 55 newborns (7.8%) had Tetanus Antibody levels