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Hatice Kati - One of the best experts on this subject based on the ideXlab platform.
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arthrobacter Pityocampae sp nov isolated from Thaumetopoea Pityocampa lep thaumetopoeidae
International Journal of Systematic and Evolutionary Microbiology, 2014Co-Authors: Ikbal Agah Ince, Zihni Demirbag, Hatice KatiAbstract:A bacterium (strain Tp2T) was isolated from a caterpillar of the pine processionary moth, Thaumetopoea Pityocampa (Den. & Schiff.) (Lepidoptera: Thaumetopoeidae), a destructive pine forest pest. The bacterium is a Gram-stain-positive, red-pigmented coccus, oxidase-negative, nitrate-reducing, non-motile and non-spore-forming. Strain Tp2T was subjected to a taxonomic study using polyphasic approach that included morphological and biochemical characterizations, 16S rRNA gene sequence analysis, DNA–DNA hybridization, DNA G+C content analysis, comparative fatty acid profiles, and analyses of quinones and polar lipids. The 16S rRNA gene sequence of strain Tp2T revealed that Arthrobacter agilis DSM 20550T was the closest known strain (98 % 16S rRNA gene sequence similarity). DNA–DNA hybridization of A. agilis DSM 20550T and strain Tp2T resulted in a DNA–DNA relatedness value of 11.9 % (20.2 % reciprocal). The DNA base composition of strain Tp2T was 69.5 mol%, which is consistent with the other recognized members of Actinobacteria that have a high G+C content in their genome. The polar lipid pattern of strain Tp2T consisted of diphosphatidylglycerol (major), phosphatidylglycerol and phosphatidylinositol and unknown glycolipids. The cellular fatty acids were anteiso C15 : 0 and anteiso C17 : 0 and the major menaquinone was MK-9(II-H2). The peptidoglycan type was A3α with an l-Lys–l-Thr–l-Ala3 interpeptide bridge. The above-mentioned characterization qualifies strain Tp2T as genotypically and phenotypically distinct from closely related species of the genus Arthrobacter with validly published names. Strain Tp2T is therefore proposed to represent a novel species of the genus Arthrobacter , described as Arthrobacter Pityocampae sp. nov. The type strain is Tp2T ( = DSM 21719T = NCCB 100254T).
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Brevibacterium Pityocampae sp. nov., isolated from caterpillars of Thaumetopoea Pityocampa (Lepidoptera, Thaumetopoeidae).
International Journal of Systematic and Evolutionary Microbiology, 2010Co-Authors: Hatice Kati, Ikbal Agah Ince, İsmail Demir, Zihni DemirbagAbstract:This work deals with the taxonomic study of a bacterium, strain Tp12(T), isolated from caterpillars of the pine processionary moth (Thaumetopoea Pityocampa Denis & Schiffermuller, 1775; Lepidoptera, Thaumetopoeidae). The isolate was assigned to the genus Brevibacterium on the basis of a polyphasic taxonomic study, including morphological and biochemical characteristics, 16S rRNA gene sequence analysis, fatty acid analysis and DNA G+C content. The highest 16S rRNA gene sequence similarity to this isolate was approximately 96 %, with the type strains of Brevibacterium album and Brevibacterium samyangense. Cellular fatty acids of the isolate are of the branched type, with the major components being anteiso-C(15 : 0) and anteiso-C(17 : 0). The DNA G+C content was 69.8 mol%. Although the strain was related to B. album and B. samyangense according to 16S rRNA gene sequence analysis, it differed from any known species of Brevibacterium. Based on this evidence, the novel species Brevibacterium Pityocampae sp. nov. is proposed, with strain Tp12(T) (=DSM 21720(T) =NCCB 100255(T)) as the type strain.
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Characterization of two Bacillus thuringiensis ssp. morrisoni strains isolated from Thaumetopoea Pityocampa (Lep., Thaumetopoeidae)
Biocontrol Science and Technology, 2009Co-Authors: Hatice Kati, Ikbal Agah Ince, Kazim Sezen, Serife Isci, Zihni DemirbagAbstract:Abstract The pine processionary moth Thaumetopoea Pityocampa Den. and Schiff. (Lep., Thaumetopoeidae) is one of the most harmful insect pests for pine species in Mediterranean countries including Turkey. Two Bacillus thuringiensis isolates obtained from T. Pityocampa were identified and characterized in terms of crystal shape using electron microscopy, SDS–PAGE analysis, cry gene contents, H-serotype and insecticidal activity. Examination by a scanning electron microscope showed that Tp6 and Tp14 isolates have flat square and bipyramidal crystal shapes, respectively. PCR analysis showed that Tp6 contains cry3 gene and Tp14 isolate contains cry1 and cry2 genes. On the other hand, the presence of Cry3 and Cry1 proteins were confirmed by observation of approximately 65- and 130-kDa proteins by SDS–PAGE in Tp6 and Tp14 isolates, respectively. According to H-serotype results, these isolates were identified as Bacillus thuringiensis ssp. morrisoni (H8a8b). Toxicity tests were performed against six insect specie...
Carole Kerdelhué - One of the best experts on this subject based on the ideXlab platform.
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Coding-Complete Genome Sequences of an Iteradensovirus and an Alphapermutotetra-Like Virus Identified from the Pine Processionary Moth (Thaumetopoea Pityocampa) in Portugal.
Microbiology resource announcements, 2021Co-Authors: Franck Dorkeld, Carole Kerdelhué, Réjane Streiff, Marie Helene OgliastroAbstract:The coding-complete genome sequences of an iteradensovirus (family Parvoviridae) and an alphapermutotetra-like virus (family Permutotetraviridae) were discovered from transcriptomic data sets obtained from Thaumetopoea Pityocampa larvae collected in Portugal. Each of the coding-complete genome sequences of these viruses contains three main open reading frames (ORFs).
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Ecological consequences of shifts in phenology in Thaumetopoea Pityocampa
2019Co-Authors: M. Branco, Carole Kerdelhué, Christian Burban, H. Santos, S. Rocha, M.-r. PaivaAbstract:Forest defoliators of temperate regions have evolutionarily adjusted their phenology to cope with food resources availability and seasonal climate fluctuations. It is critical in such environments that reproductive and developments stages coincide with suitable environmental conditions. In the pine processionary moth Thaumetopoea Pityocampa, changes in phenology can occur gradually, such as in the course of adaptation to climate with latitude and altitude, or abruptly, when mutations occur in one or more genes regulating life cycle events. Abrupt changes can either cause an eco-evolutionary dead-end, or on the contrary bring ecological opportunities, such as escape from predators, or adaptations to climate changes. Here, we report the ecological consequences of a phenological shift in a particular population of the pine processionary moth. In typical populations of this species, the adult emergence and oviposition occur in the summer, while the larval stages develop in the winter. In the shifted population, the adults mate in the spring and the larvae develop through summer. Our observations demonstrated ecological implications of the shifted life cycle and ongoing adaptations. Changes in the thermal biology, phenotypic morphological traits, fecundity and egg mortality are evidenced. Feeding constraints imposed by season and host plant physiology are also observed. Finally, competition between sympatric typical and "shifted" populations may lead to competitive exclusion. Altogether our results show a comprehensive understanding of the implications of phenology in the ecology of this pine defoliator.
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Exploration of viral communities associated with lepidoptera : A study of Thaumetopoea Pityocampa (Notodontidae) and the European corn borer species complex Ostrinia nubilalis / O. scapulalis
2018Co-Authors: Franck Dorkeld, Carole Kerdelhué, Bernard Duvic, Marie Helene OgliastroAbstract:Exploration of viral communities associated with lepidoptera : A study of Thaumetopoea Pityocampa (Notodontidae) and the European corn borer species complex Ostrinia nubilalis / O. scapulalis. 10. International Workshop on the Molecular Biology and Genetics of the Lepidoptera
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Evidence of potential hybridization in the Thaumetopoea Pityocampa-wilkinsoni complex
Agricultural and Forest Entomology, 2018Co-Authors: Edoardo Petrucco-toffolo, Carole Kerdelhué, Kahraman Ipekdal, Andrea Basso, Mauro Simonato, Andrea BattistiAbstract:1 The winter pine processionary moth complex includes some major defoliating insects of Pinus and Cedrus forests in southern Europe and the Mediterranean Basin, where they also cause health problems to humans and animals. 2 The complex includes at least two species that were separated recently based on molecular and morphological evidence: Thaumetopoea Pityocampa in the west and Thaumetopoea wilkinsoni in the east of the Mediterranean Basin. 3 Individuals from two populations, selected as representative of Th. Pityocampa and Th. wilkinsoni, were used to test whether hybridization is possible under controlled conditions. 4 The hybrid offspring showed intermediate morphological and performance traits, whereas heterosis for pupal weight was detected in one of the hybrid lines. The genetic analysis confirmed the crosses. 5 Both species have large phenological plasticity and may come into contact at the edge of their range, where they could hybridize. 6 Based on the evidence accumulated so far, it is recommended that the current species designations are maintained, although a deeper study of the trait variability is required, especially in the contact zones.
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Proteome Analysis of Urticating Setae From Thaumetopoea Pityocampa (Lepidoptera: Notodontidae).
Journal of medical entomology, 2017Co-Authors: Laura Berardi, Carole Kerdelhué, Franck Dorkeld, Micaela Pivato, Giorgio Arrigoni, E. Mitali, Anna Rita Trentin, M. Olivieri, Sabine Nidelet, Emeric DuboisAbstract:Thaumetopoea Pityocampa (Denis & Schiffermuller) (Lepidoptera: Notodontidae) is harmful to conifer trees because of defoliation and to public health because of the release of urticating setae from the caterpillars. Contact with setae by humans and domestic animals induces dermatitis, usually localized to the exposed areas. Recent studies demonstrated the presence of a complex urticating mechanism where proteins present in the setae may play a role as activators of immune responses. Yet, limited information is available at present about the proteins occurring in the setae of T. Pityocampa. Using a refined method for protein extraction from the setae, and a combination of liquid chromatography tandem-mass spectrometry (LC-MS/MS), de novo assembly of transcriptomic data, and sequence similarity searches, an extensive data set of 353 proteins was obtained. These were further categorized by molecular function, biological process, and cellular location. All the 353 proteins identified were found to match through BLAST search with at least one Lepidoptera sequence available in databases. We found the previously known allergens Tha p 1 and Tha p 2 described from T. Pityocampa, as well as enzymes involved in chitin biosynthesis, one of the principal components of the setae, and serine proteases that were responsible for inflammatory and allergic reactions in other urticating Lepidoptera. This new proteomic database may allow for a better understanding of the complexity of allergenic reactions due to T. Pityocampa and to other Lepidoptera sharing similar defense systems.
Ikbal Agah Ince - One of the best experts on this subject based on the ideXlab platform.
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arthrobacter Pityocampae sp nov isolated from Thaumetopoea Pityocampa lep thaumetopoeidae
International Journal of Systematic and Evolutionary Microbiology, 2014Co-Authors: Ikbal Agah Ince, Zihni Demirbag, Hatice KatiAbstract:A bacterium (strain Tp2T) was isolated from a caterpillar of the pine processionary moth, Thaumetopoea Pityocampa (Den. & Schiff.) (Lepidoptera: Thaumetopoeidae), a destructive pine forest pest. The bacterium is a Gram-stain-positive, red-pigmented coccus, oxidase-negative, nitrate-reducing, non-motile and non-spore-forming. Strain Tp2T was subjected to a taxonomic study using polyphasic approach that included morphological and biochemical characterizations, 16S rRNA gene sequence analysis, DNA–DNA hybridization, DNA G+C content analysis, comparative fatty acid profiles, and analyses of quinones and polar lipids. The 16S rRNA gene sequence of strain Tp2T revealed that Arthrobacter agilis DSM 20550T was the closest known strain (98 % 16S rRNA gene sequence similarity). DNA–DNA hybridization of A. agilis DSM 20550T and strain Tp2T resulted in a DNA–DNA relatedness value of 11.9 % (20.2 % reciprocal). The DNA base composition of strain Tp2T was 69.5 mol%, which is consistent with the other recognized members of Actinobacteria that have a high G+C content in their genome. The polar lipid pattern of strain Tp2T consisted of diphosphatidylglycerol (major), phosphatidylglycerol and phosphatidylinositol and unknown glycolipids. The cellular fatty acids were anteiso C15 : 0 and anteiso C17 : 0 and the major menaquinone was MK-9(II-H2). The peptidoglycan type was A3α with an l-Lys–l-Thr–l-Ala3 interpeptide bridge. The above-mentioned characterization qualifies strain Tp2T as genotypically and phenotypically distinct from closely related species of the genus Arthrobacter with validly published names. Strain Tp2T is therefore proposed to represent a novel species of the genus Arthrobacter , described as Arthrobacter Pityocampae sp. nov. The type strain is Tp2T ( = DSM 21719T = NCCB 100254T).
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Brevibacterium Pityocampae sp. nov., isolated from caterpillars of Thaumetopoea Pityocampa (Lepidoptera, Thaumetopoeidae).
International Journal of Systematic and Evolutionary Microbiology, 2010Co-Authors: Hatice Kati, Ikbal Agah Ince, İsmail Demir, Zihni DemirbagAbstract:This work deals with the taxonomic study of a bacterium, strain Tp12(T), isolated from caterpillars of the pine processionary moth (Thaumetopoea Pityocampa Denis & Schiffermuller, 1775; Lepidoptera, Thaumetopoeidae). The isolate was assigned to the genus Brevibacterium on the basis of a polyphasic taxonomic study, including morphological and biochemical characteristics, 16S rRNA gene sequence analysis, fatty acid analysis and DNA G+C content. The highest 16S rRNA gene sequence similarity to this isolate was approximately 96 %, with the type strains of Brevibacterium album and Brevibacterium samyangense. Cellular fatty acids of the isolate are of the branched type, with the major components being anteiso-C(15 : 0) and anteiso-C(17 : 0). The DNA G+C content was 69.8 mol%. Although the strain was related to B. album and B. samyangense according to 16S rRNA gene sequence analysis, it differed from any known species of Brevibacterium. Based on this evidence, the novel species Brevibacterium Pityocampae sp. nov. is proposed, with strain Tp12(T) (=DSM 21720(T) =NCCB 100255(T)) as the type strain.
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Characterization of two Bacillus thuringiensis ssp. morrisoni strains isolated from Thaumetopoea Pityocampa (Lep., Thaumetopoeidae)
Biocontrol Science and Technology, 2009Co-Authors: Hatice Kati, Ikbal Agah Ince, Kazim Sezen, Serife Isci, Zihni DemirbagAbstract:Abstract The pine processionary moth Thaumetopoea Pityocampa Den. and Schiff. (Lep., Thaumetopoeidae) is one of the most harmful insect pests for pine species in Mediterranean countries including Turkey. Two Bacillus thuringiensis isolates obtained from T. Pityocampa were identified and characterized in terms of crystal shape using electron microscopy, SDS–PAGE analysis, cry gene contents, H-serotype and insecticidal activity. Examination by a scanning electron microscope showed that Tp6 and Tp14 isolates have flat square and bipyramidal crystal shapes, respectively. PCR analysis showed that Tp6 contains cry3 gene and Tp14 isolate contains cry1 and cry2 genes. On the other hand, the presence of Cry3 and Cry1 proteins were confirmed by observation of approximately 65- and 130-kDa proteins by SDS–PAGE in Tp6 and Tp14 isolates, respectively. According to H-serotype results, these isolates were identified as Bacillus thuringiensis ssp. morrisoni (H8a8b). Toxicity tests were performed against six insect specie...
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A cytoplasmic polyhedrosis virus isolated from the pine processionary caterpillar, Thaumetopoea Pityocampa
Journal of Microbiology and Biotechnology, 2007Co-Authors: Ikbal Agah Ince, İsmail Demir, Zihni Demirbag, Remziye NalcaciogluAbstract:A cytoplasmic polyhedrosis virus (CPV) was isolated from the larvae of Thaumetopoea Pityocampa and shown to cause an infection of midgut cells. This viral infection revealed several important diagnostic symptoms, including discoloration of the posterior midgut, reduced feeding, and extended development time of the larvae. The virus infection is lethal to Thaumetopoea Pityocampa, and with the increasing doses kills the larvae within 4-5 days post infection. Electron microscopy studies showed typical cytoplasmic polyhedral inclusion bodies that are icosahedral, and ranged from 2.4 to 5.3 μm in diameter. Electrophoretic analysis of the RNA genome showed that the virus has a genome composed of 10 equimolar RNA segments with the sizes of 3,907, 3,716, 3,628, 3,249, 2,726, 1,914, 1,815, 1,256, 1,058, and 899 bp, respectively. Based on morphology and nucleic acid analysis, this virus was named Thaumetopoea Pityocampa cytoplasmic polyhedrosis virus (TpCPV), and belongs to the genus Cypovirus, family Reoviridae.
Mehmet Kanat - One of the best experts on this subject based on the ideXlab platform.
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Effect of defoliation by Thaumetopoea Pityocampa (Den. & Schiff.) (Lepidoptera: Thaumetopoeidae) on annual diameter increment of Pinus brutia Ten. in Turkey
Annals of Forest Science, 2005Co-Authors: Mehmet Kanat, M. Hakki Alma, Fatih SivrikayaAbstract:In this study, the influence of defoliation by pine processionary moth, Thaumetopoea Pityocampa (Den. & Schiff.) (Lepidoptera: Thaumetopoeidae) on the annual diameter increment of Turkish Calabrian pine, Pinus brutia Ten. in Kahramanmaras province, Turkey, was investigated. The study was carried out between 1995 and 1998. During the four years, the defoliation of pine processionary moth caused a significant decrease (average 21% for four years, P
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Effect of defoliation by Thaumetopoea Pityocampa (Den. & Schiff.) (Lepidoptera: Thaumetopoeidae) on annual diameter increment of Pinus brutia Ten. in Turkey
Annals of Forest Science, 2005Co-Authors: Mehmet Kanat, M. Hakkı Alma, Fatih SivrikayaAbstract:L'influence de defeuillaisons par la processionnaire du Pin Thaumetopoea Pityocampa, sur l'accroissement annuel en diametre de Pinus brutia, a ete testee dans la province de Kahramanmaras, en Turquie. L'etude fut conduite de 1995 a 1998. Durant ces 4 annees, les defeuillaisons ont cause une diminution significative (en moyenne 21 % pour les 4 ans, P < 0,05) de l'accroissement annuel en diametre, en comparaison avec des temoins non defeuilles.
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effect of defoliation by Thaumetopoea Pityocampa den schiff lepidoptera thaumetopoeidae on annual diameter increment of pinus brutia ten in turkey
Annals of Forest Science, 2005Co-Authors: Mehmet Kanat, Hakki M Alma, Fatih SivrikayaAbstract:L'influence de defeuillaisons par la processionnaire du Pin Thaumetopoea Pityocampa, sur l'accroissement annuel en diametre de Pinus brutia, a ete testee dans la province de Kahramanmaras, en Turquie. L'etude fut conduite de 1995 a 1998. Durant ces 4 annees, les defeuillaisons ont cause une diminution significative (en moyenne 21 % pour les 4 ans, P < 0,05) de l'accroissement annuel en diametre, en comparaison avec des temoins non defeuilles.
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insecticidal effects of essential oils from various plants against larvae of pine processionary moth Thaumetopoea Pityocampa schiff lepidoptera thaumetopoeidae
Pest Management Science, 2004Co-Authors: Mehmet Kanat, Hakki M AlmaAbstract:Along with sulfate turpentine, the essential oils obtained by steam distillation from nine plant species naturally grown in Turkish forests were tested at three different concentrations to evaluate their effectiveness against the larvae of pine processionary moth (Thaumetopoea Pityocampa Schiff). The results indicated that the essential oils from the nine species and sulfate turpentine were effective against the larvae of T Pityocampa. The most effective essential oil in the control of the larvae was steam-distilled wood turpentine, followed by thyme herb oil, juniper berry oil, laurel leaf oil, lavender flower oil, eucalyptus leaf oil, lavender leaf oil, cypress berry oil, essential oil of styrax and sulfate turpentine, respectively, in terms of mean mortality time. It is therefore feasible to use these essential oils as environment-friendly insecticides in the control of T Pityocampa.
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Insecticidal effects of essential oils from various plants against larvae of pine processionary moth (Thaumetopoea Pityocampa Schiff) (Lepidoptera: Thaumetopoeidae)
Pest Management Science, 2004Co-Authors: Mehmet Kanat, M. Hakkı AlmaAbstract:Along with sulfate turpentine, the essential oils obtained by steam distillation from nine plant species naturally grown in Turkish forests were tested at three different concentrations to evaluate their effectiveness against the larvae of pine processionary moth (Thaumetopoea Pityocampa Schiff). The results indicated that the essential oils from the nine species and sulfate turpentine were effective against the larvae of T Pityocampa. The most effective essential oil in the control of the larvae was steam-distilled wood turpentine, followed by thyme herb oil, juniper berry oil, laurel leaf oil, lavender flower oil, eucalyptus leaf oil, lavender leaf oil, cypress berry oil, essential oil of styrax and sulfate turpentine, respectively, in terms of mean mortality time. It is therefore feasible to use these essential oils as environment-friendly insecticides in the control of T Pityocampa. Copyright © 2003 Society of Chemical Industry
Zihni Demirbag - One of the best experts on this subject based on the ideXlab platform.
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Pine processionary moth (Thaumetopoea Pityocampa, Lepidoptera: Thaumetopoeidae) larvae are highly susceptible to the entomopathogenic fungi Metarhizium brunneum and Beauveria bassiana
Biocontrol Science and Technology, 2017Co-Authors: Emine Sönmez, İsmail Demir, Tariq M. Butt, James C. Bull, Zihni DemirbagAbstract:ABSTRACTThe larvae of the pine processionary moth (PPM), Thaumetopoea Pityocampa, feed on the needles of pine and cedar. The urticating hairs of older instars pose a threat to human and animal heal...
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arthrobacter Pityocampae sp nov isolated from Thaumetopoea Pityocampa lep thaumetopoeidae
International Journal of Systematic and Evolutionary Microbiology, 2014Co-Authors: Ikbal Agah Ince, Zihni Demirbag, Hatice KatiAbstract:A bacterium (strain Tp2T) was isolated from a caterpillar of the pine processionary moth, Thaumetopoea Pityocampa (Den. & Schiff.) (Lepidoptera: Thaumetopoeidae), a destructive pine forest pest. The bacterium is a Gram-stain-positive, red-pigmented coccus, oxidase-negative, nitrate-reducing, non-motile and non-spore-forming. Strain Tp2T was subjected to a taxonomic study using polyphasic approach that included morphological and biochemical characterizations, 16S rRNA gene sequence analysis, DNA–DNA hybridization, DNA G+C content analysis, comparative fatty acid profiles, and analyses of quinones and polar lipids. The 16S rRNA gene sequence of strain Tp2T revealed that Arthrobacter agilis DSM 20550T was the closest known strain (98 % 16S rRNA gene sequence similarity). DNA–DNA hybridization of A. agilis DSM 20550T and strain Tp2T resulted in a DNA–DNA relatedness value of 11.9 % (20.2 % reciprocal). The DNA base composition of strain Tp2T was 69.5 mol%, which is consistent with the other recognized members of Actinobacteria that have a high G+C content in their genome. The polar lipid pattern of strain Tp2T consisted of diphosphatidylglycerol (major), phosphatidylglycerol and phosphatidylinositol and unknown glycolipids. The cellular fatty acids were anteiso C15 : 0 and anteiso C17 : 0 and the major menaquinone was MK-9(II-H2). The peptidoglycan type was A3α with an l-Lys–l-Thr–l-Ala3 interpeptide bridge. The above-mentioned characterization qualifies strain Tp2T as genotypically and phenotypically distinct from closely related species of the genus Arthrobacter with validly published names. Strain Tp2T is therefore proposed to represent a novel species of the genus Arthrobacter , described as Arthrobacter Pityocampae sp. nov. The type strain is Tp2T ( = DSM 21719T = NCCB 100254T).
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Brevibacterium Pityocampae sp. nov., isolated from caterpillars of Thaumetopoea Pityocampa (Lepidoptera, Thaumetopoeidae).
International Journal of Systematic and Evolutionary Microbiology, 2010Co-Authors: Hatice Kati, Ikbal Agah Ince, İsmail Demir, Zihni DemirbagAbstract:This work deals with the taxonomic study of a bacterium, strain Tp12(T), isolated from caterpillars of the pine processionary moth (Thaumetopoea Pityocampa Denis & Schiffermuller, 1775; Lepidoptera, Thaumetopoeidae). The isolate was assigned to the genus Brevibacterium on the basis of a polyphasic taxonomic study, including morphological and biochemical characteristics, 16S rRNA gene sequence analysis, fatty acid analysis and DNA G+C content. The highest 16S rRNA gene sequence similarity to this isolate was approximately 96 %, with the type strains of Brevibacterium album and Brevibacterium samyangense. Cellular fatty acids of the isolate are of the branched type, with the major components being anteiso-C(15 : 0) and anteiso-C(17 : 0). The DNA G+C content was 69.8 mol%. Although the strain was related to B. album and B. samyangense according to 16S rRNA gene sequence analysis, it differed from any known species of Brevibacterium. Based on this evidence, the novel species Brevibacterium Pityocampae sp. nov. is proposed, with strain Tp12(T) (=DSM 21720(T) =NCCB 100255(T)) as the type strain.
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Characterization of two Bacillus thuringiensis ssp. morrisoni strains isolated from Thaumetopoea Pityocampa (Lep., Thaumetopoeidae)
Biocontrol Science and Technology, 2009Co-Authors: Hatice Kati, Ikbal Agah Ince, Kazim Sezen, Serife Isci, Zihni DemirbagAbstract:Abstract The pine processionary moth Thaumetopoea Pityocampa Den. and Schiff. (Lep., Thaumetopoeidae) is one of the most harmful insect pests for pine species in Mediterranean countries including Turkey. Two Bacillus thuringiensis isolates obtained from T. Pityocampa were identified and characterized in terms of crystal shape using electron microscopy, SDS–PAGE analysis, cry gene contents, H-serotype and insecticidal activity. Examination by a scanning electron microscope showed that Tp6 and Tp14 isolates have flat square and bipyramidal crystal shapes, respectively. PCR analysis showed that Tp6 contains cry3 gene and Tp14 isolate contains cry1 and cry2 genes. On the other hand, the presence of Cry3 and Cry1 proteins were confirmed by observation of approximately 65- and 130-kDa proteins by SDS–PAGE in Tp6 and Tp14 isolates, respectively. According to H-serotype results, these isolates were identified as Bacillus thuringiensis ssp. morrisoni (H8a8b). Toxicity tests were performed against six insect specie...
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A cytoplasmic polyhedrosis virus isolated from the pine processionary caterpillar, Thaumetopoea Pityocampa
Journal of Microbiology and Biotechnology, 2007Co-Authors: Ikbal Agah Ince, İsmail Demir, Zihni Demirbag, Remziye NalcaciogluAbstract:A cytoplasmic polyhedrosis virus (CPV) was isolated from the larvae of Thaumetopoea Pityocampa and shown to cause an infection of midgut cells. This viral infection revealed several important diagnostic symptoms, including discoloration of the posterior midgut, reduced feeding, and extended development time of the larvae. The virus infection is lethal to Thaumetopoea Pityocampa, and with the increasing doses kills the larvae within 4-5 days post infection. Electron microscopy studies showed typical cytoplasmic polyhedral inclusion bodies that are icosahedral, and ranged from 2.4 to 5.3 μm in diameter. Electrophoretic analysis of the RNA genome showed that the virus has a genome composed of 10 equimolar RNA segments with the sizes of 3,907, 3,716, 3,628, 3,249, 2,726, 1,914, 1,815, 1,256, 1,058, and 899 bp, respectively. Based on morphology and nucleic acid analysis, this virus was named Thaumetopoea Pityocampa cytoplasmic polyhedrosis virus (TpCPV), and belongs to the genus Cypovirus, family Reoviridae.