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Yong-ha Park - One of the best experts on this subject based on the ideXlab platform.
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proposal of the genus Thermoactinomyces sensu stricto and three new genera laceyella thermoflavimicrobium and seinonella on the basis of phenotypic phylogenetic and chemotaxonomic analyses
International Journal of Systematic and Evolutionary Microbiology, 2005Co-Authors: Jung-hoon Yoon, Yong Kook Shin, In Gi Kim, Yong-ha ParkAbstract:Phylogenetic analysis based on 16S rRNA gene sequences revealed that Thermoactinomyces species with validly published names can be assigned to four clusters or lineages. The type strains of Thermoactinomyces sacchari and Thermoactinomyces putidus were differentiated from the type strains of Thermoactinomyces vulgaris and Thermoactinomyces intermedius by the predominant menaquinone and fatty acid profiles. The type strains of Thermoactinomyces dichotomicus and Thermoactinomyces peptonophilus formed lines of descent distinct from other Thermoactinomyces species. Thermoactinomyces dichotomicus KCTC 3667T was distinguishable from the type strains of Thermoactinomyces vulgaris and Thermoactinomyces intermedius by the contents of two fatty acids, iso-C(16 : 0) and iso-C(17 : 0). Thermoactinomyces dichotomicus could be distinguished from other Thermoactinomyces species by DNA G+C content and some phenotypic properties, particularly its property of forming a yellow colour. The type strain of Thermoactinomyces peptonophilus was distinguishable from other Thermoactinomyces species by differences in menaquinone profile, major fatty acids, DNA G+C content and some physiological properties including optimal growth temperature. On the basis of these data, the creation of three new genera, Laceyella, Thermoflavimicrobium and Seinonella, is proposed in addition to the genus Thermoactinomyces sensu stricto. The genus Laceyella gen. nov. is proposed to accommodate Thermoactinomyces sacchari and Thermoactinomyces putidus as Laceyella sacchari comb. nov. and Laceyella putida comb. nov., the genus Thermoflavimicrobium gen. nov. is proposed for Thermoactinomyces dichotomicus as Thermoflavimicrobium dichotomicum comb. nov. and the genus Seinonella gen. nov. is proposed for Thermoactinomyces peptonophilus as Seinonella peptonophila comb. nov.
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dna dna relatedness among Thermoactinomyces species Thermoactinomyces candidus as a synonym of Thermoactinomyces vulgaris and Thermoactinomyces thalpophilus as a synonym of Thermoactinomyces sacchari
International Journal of Systematic and Evolutionary Microbiology, 2000Co-Authors: Jung-hoon Yoon, Yong Kook Shin, Yong-ha ParkAbstract:DNA-DNA relatedness of all validly described Thermoactinomyces species was determined to infer the genetic relationships between them. The levels of DNA-DNA relatedness among the type strains of Thermoactinomyces species ranged from 2-5 to 92.8%. Based on DNA relatedness data, the type strains of Thermoactinomyces intermedius, Thermoactinomyces putidus, Thermoactinomyces dichotomicus and Thermoactinomyces peptonophilus were considered to be distinct species of the genus Thermoactinomyces. However, the relationship between the type strains of Thermoactinomyces vulgaris and Thermoactinomyces candidus and the relationship between the type strains of Thermoactinomyces sacchari and Thermoactinomyces thalpophilus were reevaluated from levels of DNA-DNA relatedness. The independent DNA relatedness values between Thermoactinomyces vulgaris KCTC 9076T and Thermoactinomyces candidus KCTC 9557T were 90.8 and 92.8%. Thermoactinomyces thalpophilus KCTC 9789T and Thermoactinomyces sacchari KCTC 9790T exhibited independent values of 85.6 and 87.3%. Accordingly, on the basis of DNA-DNA relatedness data together with 16S rDNA sequence data determined recently, it is proposed that Thermoactinomyces candidus should be considered as a synonym of Thermoactinomyces vulgaris and Thermoactinomyces thalpophilus be considered as a synonym of Thermoactinomyces sacchari.
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phylogenetic analysis of the genus Thermoactinomyces based on 16s rdna sequences
International Journal of Systematic and Evolutionary Microbiology, 2000Co-Authors: Jung-hoon Yoon, Yong-ha ParkAbstract:The almost complete 16S rDNA sequences of the type strains of eight validly described species and two invalid species of the genus Thermoactinomyces were determined and phylogenetically analysed. The validly described Thermoactinomyces species formed phylogenetic lineages related to the family Bacillaceae, as shown previously. However, the available strains of 'Thermoactinomyces glaucus' and 'Thermoactinomyces monosporus' exhibited their closest phylogenetic affinities not to the genus Thermoactinomyces but to the genus Saccharomonospora. Some Thermoactinomyces species were shown to be closely related from 16S rDNA sequence analysis. Particularly, Thermoactinomyces vulgaris KCTC 9076T and Thermoactinomyces candidus KCTC 9557T had the same 16S rDNA sequences and Thermoactinomyces thalpophilus KCTC 9789T and Thermoactinomyces sacchari KCTC 9790T showed 16S rDNA similarity value of almost 100%. From phylogenetic analysis based on 16S rDNA sequences, it is suggested that the genus Thermoactinomyces should be taxonomically re-evaluated using other useful taxonomic markers.
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DNA-DNA relatedness among Thermoactinomyces species: Thermoactinomyces candidus as a synonym of Thermoactinomyces vulgaris and Thermoactinomyces thalpophilus as a synonym of Thermoactinomyces sacchari
Society for General Microbiology, 2000Co-Authors: Jung-hoon Yoon, Yong Kook Shin, Yong-ha ParkAbstract:DNA-DNA relatedness of all validly described Thermoactinomyces species was determined to infer the genetic relationships between them. The levels of DNA-DNA relatedness among the type strains of Thermoactinomyces species ranged from 2.5 to 92.8%. Based on DNA relatedness data, the type strains of Thermoactinomyces intermedius, Thermoactinomyces putidus, Thermoactinomyces dichotomicus and Thermoactinomyces peptonophilus were considered to be distinct species of the genus Thermoactinomyces. However, the relationship between the type strains of Thermoactinomyces vulgaris and Thermoactinomyces candidus and the relationship between the type strains of Thermoactinomyces sacchari and Thermoactinomyces thalpophilus were reevaluated from levels of DNA-DNA relatedness. The independent DNA relatedness values between Thermoactinomyces vulgaris KCTC 9076(T) and Thermoactinomyces candidus KCTC 9557(T) were 90.8 and 92.8%. Thermoactinomyces thalpophilus KCTC 9789(T) and Thermoactinomyces sacchari KCTC 9790(T) exhibited independent values of 85.6 and 87.3%. Accordingly, on the basis of DNA-DNA relatedness data together with 16S rDNA sequence data determined recently, it is proposed that Thermoactinomyces candidus should be considered as a synonym of Thermoactinomyces vulgaris and Thermoactinomyces thalpophilus be considered as a synonym of Thermoactinomyces sacchari.ope
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Phylogenetic analysis of the genus Thermoactinomyces based on 16S rDNA sequences
Society for General Microbiology, 2000Co-Authors: Jung-hoon Yoon, Yong-ha ParkAbstract:The almost complete 16S rDNA sequences of the type strains of eight validly described species and two invalid species of the genus Thermoactinomyces were determined and phylogenetically analysed. The validly described Thermoactinomyces species formed phylogenetic lineages related to the family Bacillaceae, as shown previously. However, the available strains of 'Thermoactinomyces glaucus' and 'Thermoactinomyces monosporus' exhibited their closest phylogenetic affinities not to the genus Thermoactinomyces but to the genus Saccharomonospora. Some Thermoactinomyces species were shown to be closely related from 16S rDNA sequence analysis. Particularly, Thermoactinomyces vulgaris KCTC 9076(T) and Thermoactinomyces candidus KCTC 9557(T) had the same 16S rDNA sequences and Thermoactinomyces thalpophilus KCTC 9789(T) and Thermoactinomyces sacchari KCTC 9790(T) showed 16S rDNA similarity value of almost 100%. From phylogenetic analysis based on 16S rDNA sequences, it is suggested that the genus Thermoactinomyces should be taxonomically re-evaluated using other useful taxonomic markers.ope
Amit Verma - One of the best experts on this subject based on the ideXlab platform.
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production of thermostable organic solvent tolerant keratinolytic protease from Thermoactinomyces sp rm4 iaa production and plant growth promotion
Frontiers in Microbiology, 2016Co-Authors: Amit Verma, Hukum Singh, Mohammad S Anwar, Shailendra Kumar, Mohammad Ansari, Sanjeev AgrawalAbstract:There are several reports about the optimization of protease production, but only few have optimized the production of organic solvent tolerant keratinolytic proteases that show remarkable exploitation in the development of the non-polluting processes in biotechnological industries. The present study was carried with aim to optimize the production of a thermostable organic solvent tolerant keratinolytic protease Thermoactinomyces sp. RM4 utilizing chicken feathers. Thermoactinomyces sp. RM4 isolated from the soil sample collected from a rice mill wasteyard site near Kashipur, Uttrakhand was identified on the basis of 16S rDNA analysis. The production of organic solvent tolerant keratinolytic protease enzyme by Thermoactinomyces sp. RM4 was optimized by varying physical culture conditions such as pH (10.0), temperature (60°C), inoculum percentage (2%), feather concentration (2%) and agitation rate (2g) for feather degradation. The result showed that Thermoactinomyces sp. RM4 potentially produces extra-cellular thermostable organic solvent tolerant keratinolytic protease in the culture medium. Further, the feather hydrolysate from keratinase production media showed plant growth promoting activity by producing indole-3-acetic acid (IAA) itself. The present findings suggest that keratinolytic protease from Thermoactinomyces sp. RM4 offers enormous industrial applications due to its organic solvent tolerant property in peptide synthesis, practical role in feather degradation and potential function in plant growth promoting activity, which might be a superior candidate to keep ecosystem healthy and functional.
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alkaline protease from Thermoactinomyces sp rs1 mitigates industrial pollution
Protoplasma, 2014Co-Authors: Amit Verma, Mohammad Ansari, Mohmmad Shahbaz Anwar, Ruchi Agrawal, Sanjeev AgrawalAbstract:Proteases have found a wide application in the several industrial processes, such as laundry detergents, protein recovery or solubilization, prion degradation, meat tenderizations, and in bating of hides and skins in leather industries. But the main hurdle in industrial application of proteases is their economical production on a large scale. The present investigation aimed to exploit the locally available inexpensive agricultural and household wastes for alkaline protease production using Thermoactinomyces sp. RS1 via solid-state fermentation (SSF) technique. The alkaline enzyme is potentially useful as an additive in commercial detergents to mitigate pollution load due to extensive use of caustic soda-based detergents. Thermoactinomyces sp. RS1 showed good protease production under SSF conditions of 55 °C, pH 9, and 50 % moisture content with potato peels as solid substrate. The presented findings revealed that crude alkaline protease produced by Thermoactinomyces sp. RS1 via SSF is of potential application in silver recovery from used X-ray films.
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Potential of Alkaline Protease Isolated from Thermoactinomyces sp. RM4 as an Alternative to Conventional Chemicals in Leather Industry Dehairing Process
Jr. of Agril. Env. and Biotech. Microbiology, 2011Co-Authors: Amit Verma, Hukum Singh Pal, Rachna Singh, Sanjeev AgarwalAbstract:Alkaline proteases have found a wide application in several industrial processes, such as laundry detergents, protein recovery or solubilization, prion degradation, meat tenderizations, in bating of hides and skins in leather industries. The conventional methods in leather processing involve the use of sulfide, lime and other chemicals, creating environmental pollution and safety hazards. Thus, for environmental reasons, the biotreament of leather using an enzymatic approach is preferable as it offers several advantages, e.g. easy control, speed and waste reduction, thus being ecofriendly. In present study, a bacterial isolate Thermoactinomyces sp. RM4, isolated from soil sample by spread plating method on nutrient agar (pH 8) at 60.0°C. The optimum pH and temperature of the enzyme isolated from Thermoactinomyces sp. RM4 was found to be 10.0 and 80.0°C, respectively and retains 45% of its activity at 90°C for 3 hours. This isolate Thermoactinomyces sp. RM4 shows good dehairing of hide within short incubation time, intact hair recovery and clean pelt.
Jung-hoon Yoon - One of the best experts on this subject based on the ideXlab platform.
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proposal of the genus Thermoactinomyces sensu stricto and three new genera laceyella thermoflavimicrobium and seinonella on the basis of phenotypic phylogenetic and chemotaxonomic analyses
International Journal of Systematic and Evolutionary Microbiology, 2005Co-Authors: Jung-hoon Yoon, Yong Kook Shin, In Gi Kim, Yong-ha ParkAbstract:Phylogenetic analysis based on 16S rRNA gene sequences revealed that Thermoactinomyces species with validly published names can be assigned to four clusters or lineages. The type strains of Thermoactinomyces sacchari and Thermoactinomyces putidus were differentiated from the type strains of Thermoactinomyces vulgaris and Thermoactinomyces intermedius by the predominant menaquinone and fatty acid profiles. The type strains of Thermoactinomyces dichotomicus and Thermoactinomyces peptonophilus formed lines of descent distinct from other Thermoactinomyces species. Thermoactinomyces dichotomicus KCTC 3667T was distinguishable from the type strains of Thermoactinomyces vulgaris and Thermoactinomyces intermedius by the contents of two fatty acids, iso-C(16 : 0) and iso-C(17 : 0). Thermoactinomyces dichotomicus could be distinguished from other Thermoactinomyces species by DNA G+C content and some phenotypic properties, particularly its property of forming a yellow colour. The type strain of Thermoactinomyces peptonophilus was distinguishable from other Thermoactinomyces species by differences in menaquinone profile, major fatty acids, DNA G+C content and some physiological properties including optimal growth temperature. On the basis of these data, the creation of three new genera, Laceyella, Thermoflavimicrobium and Seinonella, is proposed in addition to the genus Thermoactinomyces sensu stricto. The genus Laceyella gen. nov. is proposed to accommodate Thermoactinomyces sacchari and Thermoactinomyces putidus as Laceyella sacchari comb. nov. and Laceyella putida comb. nov., the genus Thermoflavimicrobium gen. nov. is proposed for Thermoactinomyces dichotomicus as Thermoflavimicrobium dichotomicum comb. nov. and the genus Seinonella gen. nov. is proposed for Thermoactinomyces peptonophilus as Seinonella peptonophila comb. nov.
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dna dna relatedness among Thermoactinomyces species Thermoactinomyces candidus as a synonym of Thermoactinomyces vulgaris and Thermoactinomyces thalpophilus as a synonym of Thermoactinomyces sacchari
International Journal of Systematic and Evolutionary Microbiology, 2000Co-Authors: Jung-hoon Yoon, Yong Kook Shin, Yong-ha ParkAbstract:DNA-DNA relatedness of all validly described Thermoactinomyces species was determined to infer the genetic relationships between them. The levels of DNA-DNA relatedness among the type strains of Thermoactinomyces species ranged from 2-5 to 92.8%. Based on DNA relatedness data, the type strains of Thermoactinomyces intermedius, Thermoactinomyces putidus, Thermoactinomyces dichotomicus and Thermoactinomyces peptonophilus were considered to be distinct species of the genus Thermoactinomyces. However, the relationship between the type strains of Thermoactinomyces vulgaris and Thermoactinomyces candidus and the relationship between the type strains of Thermoactinomyces sacchari and Thermoactinomyces thalpophilus were reevaluated from levels of DNA-DNA relatedness. The independent DNA relatedness values between Thermoactinomyces vulgaris KCTC 9076T and Thermoactinomyces candidus KCTC 9557T were 90.8 and 92.8%. Thermoactinomyces thalpophilus KCTC 9789T and Thermoactinomyces sacchari KCTC 9790T exhibited independent values of 85.6 and 87.3%. Accordingly, on the basis of DNA-DNA relatedness data together with 16S rDNA sequence data determined recently, it is proposed that Thermoactinomyces candidus should be considered as a synonym of Thermoactinomyces vulgaris and Thermoactinomyces thalpophilus be considered as a synonym of Thermoactinomyces sacchari.
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phylogenetic analysis of the genus Thermoactinomyces based on 16s rdna sequences
International Journal of Systematic and Evolutionary Microbiology, 2000Co-Authors: Jung-hoon Yoon, Yong-ha ParkAbstract:The almost complete 16S rDNA sequences of the type strains of eight validly described species and two invalid species of the genus Thermoactinomyces were determined and phylogenetically analysed. The validly described Thermoactinomyces species formed phylogenetic lineages related to the family Bacillaceae, as shown previously. However, the available strains of 'Thermoactinomyces glaucus' and 'Thermoactinomyces monosporus' exhibited their closest phylogenetic affinities not to the genus Thermoactinomyces but to the genus Saccharomonospora. Some Thermoactinomyces species were shown to be closely related from 16S rDNA sequence analysis. Particularly, Thermoactinomyces vulgaris KCTC 9076T and Thermoactinomyces candidus KCTC 9557T had the same 16S rDNA sequences and Thermoactinomyces thalpophilus KCTC 9789T and Thermoactinomyces sacchari KCTC 9790T showed 16S rDNA similarity value of almost 100%. From phylogenetic analysis based on 16S rDNA sequences, it is suggested that the genus Thermoactinomyces should be taxonomically re-evaluated using other useful taxonomic markers.
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DNA-DNA relatedness among Thermoactinomyces species: Thermoactinomyces candidus as a synonym of Thermoactinomyces vulgaris and Thermoactinomyces thalpophilus as a synonym of Thermoactinomyces sacchari
Society for General Microbiology, 2000Co-Authors: Jung-hoon Yoon, Yong Kook Shin, Yong-ha ParkAbstract:DNA-DNA relatedness of all validly described Thermoactinomyces species was determined to infer the genetic relationships between them. The levels of DNA-DNA relatedness among the type strains of Thermoactinomyces species ranged from 2.5 to 92.8%. Based on DNA relatedness data, the type strains of Thermoactinomyces intermedius, Thermoactinomyces putidus, Thermoactinomyces dichotomicus and Thermoactinomyces peptonophilus were considered to be distinct species of the genus Thermoactinomyces. However, the relationship between the type strains of Thermoactinomyces vulgaris and Thermoactinomyces candidus and the relationship between the type strains of Thermoactinomyces sacchari and Thermoactinomyces thalpophilus were reevaluated from levels of DNA-DNA relatedness. The independent DNA relatedness values between Thermoactinomyces vulgaris KCTC 9076(T) and Thermoactinomyces candidus KCTC 9557(T) were 90.8 and 92.8%. Thermoactinomyces thalpophilus KCTC 9789(T) and Thermoactinomyces sacchari KCTC 9790(T) exhibited independent values of 85.6 and 87.3%. Accordingly, on the basis of DNA-DNA relatedness data together with 16S rDNA sequence data determined recently, it is proposed that Thermoactinomyces candidus should be considered as a synonym of Thermoactinomyces vulgaris and Thermoactinomyces thalpophilus be considered as a synonym of Thermoactinomyces sacchari.ope
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Phylogenetic analysis of the genus Thermoactinomyces based on 16S rDNA sequences
Society for General Microbiology, 2000Co-Authors: Jung-hoon Yoon, Yong-ha ParkAbstract:The almost complete 16S rDNA sequences of the type strains of eight validly described species and two invalid species of the genus Thermoactinomyces were determined and phylogenetically analysed. The validly described Thermoactinomyces species formed phylogenetic lineages related to the family Bacillaceae, as shown previously. However, the available strains of 'Thermoactinomyces glaucus' and 'Thermoactinomyces monosporus' exhibited their closest phylogenetic affinities not to the genus Thermoactinomyces but to the genus Saccharomonospora. Some Thermoactinomyces species were shown to be closely related from 16S rDNA sequence analysis. Particularly, Thermoactinomyces vulgaris KCTC 9076(T) and Thermoactinomyces candidus KCTC 9557(T) had the same 16S rDNA sequences and Thermoactinomyces thalpophilus KCTC 9789(T) and Thermoactinomyces sacchari KCTC 9790(T) showed 16S rDNA similarity value of almost 100%. From phylogenetic analysis based on 16S rDNA sequences, it is suggested that the genus Thermoactinomyces should be taxonomically re-evaluated using other useful taxonomic markers.ope
Sanjeev Agrawal - One of the best experts on this subject based on the ideXlab platform.
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production of thermostable organic solvent tolerant keratinolytic protease from Thermoactinomyces sp rm4 iaa production and plant growth promotion
Frontiers in Microbiology, 2016Co-Authors: Amit Verma, Hukum Singh, Mohammad S Anwar, Shailendra Kumar, Mohammad Ansari, Sanjeev AgrawalAbstract:There are several reports about the optimization of protease production, but only few have optimized the production of organic solvent tolerant keratinolytic proteases that show remarkable exploitation in the development of the non-polluting processes in biotechnological industries. The present study was carried with aim to optimize the production of a thermostable organic solvent tolerant keratinolytic protease Thermoactinomyces sp. RM4 utilizing chicken feathers. Thermoactinomyces sp. RM4 isolated from the soil sample collected from a rice mill wasteyard site near Kashipur, Uttrakhand was identified on the basis of 16S rDNA analysis. The production of organic solvent tolerant keratinolytic protease enzyme by Thermoactinomyces sp. RM4 was optimized by varying physical culture conditions such as pH (10.0), temperature (60°C), inoculum percentage (2%), feather concentration (2%) and agitation rate (2g) for feather degradation. The result showed that Thermoactinomyces sp. RM4 potentially produces extra-cellular thermostable organic solvent tolerant keratinolytic protease in the culture medium. Further, the feather hydrolysate from keratinase production media showed plant growth promoting activity by producing indole-3-acetic acid (IAA) itself. The present findings suggest that keratinolytic protease from Thermoactinomyces sp. RM4 offers enormous industrial applications due to its organic solvent tolerant property in peptide synthesis, practical role in feather degradation and potential function in plant growth promoting activity, which might be a superior candidate to keep ecosystem healthy and functional.
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alkaline protease from Thermoactinomyces sp rs1 mitigates industrial pollution
Protoplasma, 2014Co-Authors: Amit Verma, Mohammad Ansari, Mohmmad Shahbaz Anwar, Ruchi Agrawal, Sanjeev AgrawalAbstract:Proteases have found a wide application in the several industrial processes, such as laundry detergents, protein recovery or solubilization, prion degradation, meat tenderizations, and in bating of hides and skins in leather industries. But the main hurdle in industrial application of proteases is their economical production on a large scale. The present investigation aimed to exploit the locally available inexpensive agricultural and household wastes for alkaline protease production using Thermoactinomyces sp. RS1 via solid-state fermentation (SSF) technique. The alkaline enzyme is potentially useful as an additive in commercial detergents to mitigate pollution load due to extensive use of caustic soda-based detergents. Thermoactinomyces sp. RS1 showed good protease production under SSF conditions of 55 °C, pH 9, and 50 % moisture content with potato peels as solid substrate. The presented findings revealed that crude alkaline protease produced by Thermoactinomyces sp. RS1 via SSF is of potential application in silver recovery from used X-ray films.
Howard W Larsh - One of the best experts on this subject based on the ideXlab platform.
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chemical and serological comparison of two antigen extracts of Thermoactinomyces candidus
Mycoses, 2009Co-Authors: Gary E Hollick, Nancy K Hall, Howard W LarshAbstract:Summary: Immunologically reactive fractions were isolated from two antigen extracts of Thermoactinomyces candidus (Thermoactinomyces pyridine extract-TPE and double dialysis antigen-DDA). Activity was demonstrated using immunoelectrophoresis and crossed immunoelectrophoresis against rabbit antisera to T. candidus spores. Chemical analysis of DDA revealed 11 to 16 bands on polyacrylamide gel electrophoresis including five glycoprotein bands. TPE contained 8 to 11 protein bands with 3 glycoprotein components. Both antigens showed variation in sequential preparations. Zusammenfassung: Aus 2 Antigenextrakten von Thermoactinomyces candidus (Thermoactinomyces-Pyridin-Extrakt [TPE] und Doppeldialyse-Antigen [DDA]) wur-den die immunologisch reaktiven Fraktionen isoliert. Mittels Immunelektrophorese und zweidimensionaler Immunelektrophorese wurde ihre Aktivitat gegen T.-candidus-Sporen-Antiserum vom Kaninchen nachgewiesen. Die chemische Analyse von DDA ergab bei der Polyacrylamid-Gel-Elektrophorese 11 bis 16 Streifen (Bande) einschliefi-lich 5 Glukoprotein-Streifen. TPE enthielt 8–11 Proteinstreifen mit 3 Glukoprotein-Komponenten. Beide Antigene zeigten bei Sequenzversuchen mit neuen Praparationen Variationserscheinungen.
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peripheral and alveolar response in guinea pigs to an aerosol exposure of Thermoactinomyces candidus spores periphere und alveolare immunantwort in meerschweinchen nach aerosol exposition mit Thermoactinomyces candidus sporen
Mycoses, 2009Co-Authors: Gary E Hollick, Nancy K Hall, Howard W LarshAbstract:Summary: The development of peripheral and alveolar cell mediated immunity (CMI) in guinea pigs was studied after an aerosol sensitization with Thermoactinomyces candidus spores. Skin tests, the production of macrophage inhibition factor, and lymphocyte transformation studies revealed no development of CMI in the peripheral system. Cell stimulation studies using alveolar cells revealed the development of CMI after the second exposure. However, the reactivity diminished following the third exposure. This occurred in spite of the fact that the total number of alveolar cells remained significantly above control animal cell counts. The possible relationship between these findings and individuals with asymptomatic hypersensitivity pneumonitis is discussed. Zusammenfassung: An Meerschweinchen wurde die Entwicklung der peripheren und alveolaren zellvermittelten Immunantwort nach Sensibilisierung mit Sporen von Thermoactinomyces candidus in einem Aerosol untersucht. Im peripheren System konnte die Entwicklung einer zellvermittelten Immunantwort weder durch Hautteste, noch durch die Produktion von Makrophagen inhibierendem Faktor noch im Lymphozyten-Transformationstest nachgewiesen werden. Dagegen zeigten Untersuchungen zum Nachweis einer spezifischen Stimulierung alveolarer Zellen die Entwicklung einer zellvermittelten Immunantwort nach der zweiten Exposition. Die Reaktivitat nahm jedoch im Anschlus an eine dritte Exposition wieder ab. Dieser Ruckgang der Reaktivitat entwickelte sich, obwohl die Gesamtzahl der alveolaren Zellen signifikant hoher blieb als die Zellzahl in Kontrolltieren. Die mogliche Bedeutung dieser Befunde fur das Verstandnis der Vorgange bei Personen mit asymptomatischer Hypersensivitatspneumonie wird diskutiert.