The Experts below are selected from a list of 780 Experts worldwide ranked by ideXlab platform
Lizardo Inicio, Katherine Yuliana - One of the best experts on this subject based on the ideXlab platform.
-
Colonizacion bacteriana en conos de papel utilizados por los estudiantes de estomatologia en sus terapias pulpares
Universidad Privada Antenor Orrego - UPAO, 2017Co-Authors: Lizardo Inicio, Katherine YulianaAbstract:Objetive: To determine the bacterial colonizat ion on the paper cones used by stomatology students of the Universidad Privada Antenor Orrego in their endodontic therapies in 2016. Materials and Methods: A prospective, cross-curricular, descriptive and observat ional study was carried out. A total of 290 samples of paper cones collected from a total of 145 stomatology students were evaluated during their endodontic therapies in endodontics I, endodontics II, integral clinic I, integral clinical II and stomatologic internship. Students were asked to let us randomly select 2 paper cones, which were collected after the student would use them to dry their root canal in their endodontic treatment. These cones wer e placed in test tubes containing Thioglycollate Broth and immediately transferred to the Mult idisciplinary Research Laboratory (LABINM) of the Universidad Privada Antenor Orrego to be incubated at 37 ° C for 16 and 24 hours. An inoculum was then obtained from the medium of each test tube, which was seeded in a petri dish containing blood agar supplemented with 5% percent of human blood and on a MacConkey agar plate respectively. Both plates were then incubated at 37 ° C for 24 hours to check for bacterial growth by observation of bacterial colony forming units. The collected data were expressed in frequencies and percentages using descriptive and inferent ial statist ics, as well as the nonparametric Kruskal Wallis test for the comparison of the groups. Data processing was performed using Excel and IBM SPSS softwares. The results were presented in tables of frequencies and statist ical graphs.Objetivo: Determinar la colonización bacteriana en los conos de papel utilizados por los estudiantes de estomatología de la Universidad Privada Antenor Orrego en sus terapias endodónticas en el año 2016. Materiales y Método: Se realizó un estudio prospectivo, transversal, descriptivo y observacional. Se evaluaron 290 muestras de conos de papel recolectados de un total de 145 estudiantes de estomatología durante la realización de sus terapias endodónticas en las asignaturas de endodoncia I, endodoncia II, clínica integral I, clínica integral II e internado estomatológico. Se solicitó a los estudiantes, nos permitiera escoger al azar 2 conos de papel, los cuáles fueron recolectados en el momento que el estudiante los utilizaría para secar sus conductos radiculares en su tratamiento endodóntico. Dichos conos fueron colocados en tubos de ensayo que contenían caldo tioglicolato, inmediatamente después fueron trasladados al Laboratorio de Investigación Multidisciplinaria (LABINM) de la Universidad Privada Antenor Orrego para ser incubados a 37° C durante 16 y 24 horas. Posteriormente se obtuvo un inóculo del medio de cada tubo de ensayo, el cual se sembró en una placa petri que contenía agar BHI suplementado con 5% de sangre humana y en una placa de agar MacConkey respectivamente. Luego ambas placas se incubaron a 37°C durante 24 horas para verificar si hubo crecimiento bacteriano mediante la observación de unidades formadoras de colonias bacterianas. Los datos recolectados fueron expresados en frecuencias y porcentajes utilizando estadística descriptiva e inferencial, así como también se empleó la prueba no paramétrica Kruskal Wallis para la comparación de los grupos. El procesamiento de los datos se realizó utilizando softwares Excel e IBM SPSS. Los resultados se presentaron en tablas de frecuencias y gráficos estadísticos
-
Colonización bacteriana en conos de papel utilizados por los estudiantes de estomatología en sus terapias pulpares
Universidad Privada Antenor Orrego - UPAO, 2017Co-Authors: Lizardo Inicio, Katherine YulianaAbstract:Objetivo: Determinar la colonización bacteriana en los conos de papel utilizados por los estudiantes de estomatología de la Universidad Privada Antenor Orrego en sus terapias endodónticas en el año 2016. Materiales y Método: Se realizó un estudio prospectivo, transversal, descriptivo y observacional. Se evaluaron 290 muestras de conos de papel recolectados de un total de 145 estudiantes de estomatología durante la realización de sus terapias endodónticas en las asignaturas de endodoncia I, endodoncia II, clínica integral I, clínica integral II e internado estomatológico. Se solicitó a los estudiantes, nos permitiera escoger al azar 2 conos de papel, los cuáles fueron recolectados en el momento que el estudiante los utilizaría para secar sus conductos radiculares en su tratamiento endodóntico. Dichos conos fueron colocados en tubos de ensayo que contenían caldo tioglicolato, inmediatamente después fueron trasladados al Laboratorio de Investigación Multidisciplinaria (LABINM) de la Universidad Privada Antenor Orrego para ser incubados a 37° C durante 16 y 24 horas. Posteriormente se obtuvo un inóculo del medio de cada tubo de ensayo, el cual se sembró en una placa petri que contenía agar BHI suplementado con 5% de sangre humana y en una placa de agar MacConkey respectivamente. Luego ambas placas se incubaron a 37°C durante 24 horas para verificar si hubo crecimiento bacteriano mediante la observación de unidades formadoras de colonias bacterianas. Los datos recolectados fueron expresados en frecuencias y porcentajes utilizando estadística descriptiva e inferencial, así como también se empleó la prueba no paramétrica Kruskal Wallis para la comparación de los grupos. El procesamiento de los datos se realizó utilizando softwares Excel e IBM SPSS. Los resultados se presentaron en tablas de frecuencias y gráficos estadísticos.Objetive: To determine the bacterial colonizat ion on the paper cones used by stomatology students of the Universidad Privada Antenor Orrego in their endodontic therapies in 2016. Materials and Methods: A prospective, cross-curricular, descriptive and observat ional study was carried out. A total of 290 samples of paper cones collected from a total of 145 stomatology students were evaluated during their endodontic therapies in endodontics I, endodontics II, integral clinic I, integral clinical II and stomatologic internship. Students were asked to let us randomly select 2 paper cones, which were collected after the student would use them to dry their root canal in their endodontic treatment. These cones wer e placed in test tubes containing Thioglycollate Broth and immediately transferred to the Mult idisciplinary Research Laboratory (LABINM) of the Universidad Privada Antenor Orrego to be incubated at 37 ° C for 16 and 24 hours. An inoculum was then obtained from the medium of each test tube, which was seeded in a petri dish containing blood agar supplemented with 5% percent of human blood and on a MacConkey agar plate respectively. Both plates were then incubated at 37 ° C for 24 hours to check for bacterial growth by observation of bacterial colony forming units. The collected data were expressed in frequencies and percentages using descriptive and inferent ial statist ics, as well as the nonparametric Kruskal Wallis test for the comparison of the groups. Data processing was performed using Excel and IBM SPSS softwares. The results were presented in tables of frequencies and statist ical graphs.Tesi
Johnson Rajeswar - One of the best experts on this subject based on the ideXlab platform.
-
Alpha toxin specific PCR for detection of toxigenic strains of Clostridium perfringens in Poultry
2013Co-Authors: Malmarugan Shanmugasamy, Johnson RajeswarAbstract:Aim: Isolation of Clostridium perfringens from necrotic enteritis cases in poultry and confirmation by alpha toxin specific PCR Materials and methods: Robertson cooked meat medium with Brain Heart Infusion Broth was used for isolation of C. perfringens from intestinal contents of necrotic enteritis suspected birds. Positive cultures from perfringens agar were further confirmed by biochemical tests and subjected to alpha toxin specific PCR. Results and Discussion: Twenty Clostridium perfringens isolates were isolated from intestinal contents of thirty five NE suspected birds. Out of the twenty isolates, fourteen were isolated from commercial broilers of 2 to 6 wk of age and six from commercial layers of 9 to 15 wk of age. Frequency of isolation of C. perfringens was more with Robertson cooked meat medium with BHI Broth than Thioglycollate Broth alone. When positive cultures were streaked on to clostridial agar appreciable luxuriant growths were obtained and the selective streaking of these colonies on perfringens agar with supplements revealed rough and black colonies with sulphate reduction. The isolates produced rough and black colonies with sulphate reduction on perfringens agar, double zone haemolysis on sheep blood agar, stormy clot fermentation on milk medium and opalescence on egg yolk medium. The isolates were found negative for oxidase, catalase, liquefied gelatin, fermented glucose, maltose, lactose and sucrose except mannitol. All the fourteen isolates obtained from commercial broilers proved the alpha toxin producing strains of C. perfringens when they were subjected to alpha toxin specific PCR. Conclusion: This study revealed alpha toxin specific PCR is highly useful for detection of toxigenic strains of Clostridium perfringens in poultr
-
Alpha toxin specific PCR for detection of toxigenic strains of Clostridium perfringens in Poultry
Veterinary World, 2012Co-Authors: Malmarugan Shanmugasamy, Johnson RajeswarAbstract:Aim : Isolation of clostridium perfirngens from necrotic enteritis cases in poultry and confirmation by alpha toxin specific PCR Materials and methods: Robertson cooked meat medium with Brain Heart Infusion Broth was used for isolation of C. perfringens from intestinal contents of necrotic enteritis suspected birds. Positive cultures from perfringens agar were further confirmed by biochemical tests and subjected to alpha toxin specific PCR. Results: Twenty Clostridium perfringens isolates were isolated from intestinal contents of thirty five NE suspected birds. Out of the twenty isolates, fourteen were isolated from commercial broilers of 2 to 6 wk of age and six from commercial layers of 9 to 15 wk of age. Frequency of isolation of C. perfringens was more with Robertson cooked meat medium with BHI Broth than Thioglycollate Broth alone. When positive cultures were streaked on to clostridial agar appreciable luxuriant growths were obtained and the selective streaking of these colonies on perfringens agar with supplements revealed rough and black colonies with sulphate reduction. The isolates produced rough and black colonies with sulphate reduction on perfringens agar, double zone haemolysis on sheep blood agar, stormy clot fermentation on milk medium and opalescence on egg yolk medium. The isolates were found negative for oxidase, catalase, liquefied gelatin, fermented glucose, maltose, lactose and sucrose except mannitol. All the fourteen isolates obtained from commercial broilers proved the alpha toxin producing strains of C. perfringens when they were subjected to alpha toxin specific PCR. Conclusion : This study revealed alpha toxin specific PCR is highly useful for detection of toxigenic strains of Clostridium perfringens in poultry [Vet. World 2012; 5(6.000): 365-368
Raheleh Karimiravesh - One of the best experts on this subject based on the ideXlab platform.
-
Original Article Inhibitory Effect of Lactobacillus rhamnosus on Pathogenic Bacteria Isolated from Women with Bacterial Vaginosis
2016Co-Authors: Gita Eslami, Sudabeh Taheri, Eznollah Azargashb, Raheleh KarimiraveshAbstract:Background: Considering the high prevalence of bacterial vaginosis and its association with urinary tract infection in women and treatment of gynecologic problems occur when a high recurrence of bacterial vaginosis is often treated with antibiotics. The purpose of this study is to investigate the inhibitory effect of Lactobacillus rhamnosus on pathogenic bacteria isolated from women with bacterial vaginosis, respectively. Materials and Methods: 96 samples from women with bacterial vaginosis discharge referred to health centers dependent Shahid Beheshti University in 91-92 were taken by a gynecologist with a dacron swab and put in sterile tubes containing TSB Broth and Thioglycollate Broth and were immediately sent to the lab location in cold chain for the next stages of investigation. From Thioglycollate and TSB medium was cultured on blood agar and EMB and Palkam and Differential diagnosis environments, and then incubated for 24 h at 37°C. Strains of Lactobacillus rhamnosus were cultured in MRSA environment and were transfered to the lab. After purification of pathogenic bacteria, MIC methods and antibiogram, Lactobacillus rhamnosus inhibitory effect on pathogenic bacteria is checked. Statistical analysis was done by SPSS software v.16. Results: The results of this study show the inhibitory effect of Lactobacillus rhamnosus on some pathogenic bacteria that cause bacterial vaginosis, including Staphylococcus aureus, Staphylococcus epidermidis
-
Inhibitory effect of Lactobacillus rhamnosus on pathogenic bacteria isolated from women with bacterial vaginosis
Shahid Beheshti University of Medical Sciences, 2014Co-Authors: Gita Eslami, Sudabeh Taheri, Eznollah Azargashb, Raheleh KarimiraveshAbstract:Background: Considering the high prevalence of bacterial vaginosis and its association with urinary tract infection in women and treatment of gynecologic problems occur when a high recurrence of bacterial vaginosis is often treated with antibiotics. The purpose of this study is to investigate the inhibitory effect of Lactobacillus rhamnosus on pathogenic bacteria isolated from women with bacterial vaginosis, respectively.Materials and Methods: 96 samples from women with bacterial vaginosis discharge referred to health centers dependent Shahid Beheshti University in 91-92 were taken by a gynecologist with a dacron swab and put in sterile tubes containing TSB Broth and Thioglycollate Broth and were immediately sent to the lab location in cold chain for the next stages of investigation. From Thioglycollate and TSB medium was cultured on blood agar and EMB and Palkam and Differential diagnosis environments, and then incubated for 24 h at 37°C. Strains of Lactobacillus rhamnosus were cultured in MRSA environment and were transfered to the lab. After purification of pathogenic bacteria, MIC methods and antibiogram, Lactobacillus rhamnosus inhibitory effect on pathogenic bacteria is checked. Statistical analysis was done by SPSS software v.16.Results: The results of this study show the inhibitory effect of Lactobacillus rhamnosus on some pathogenic bacteria that cause bacterial vaginosis, including Staphylococcus aureus, Staphylococcus epidermidis, Staphylococcus saprophyticus, Streptococcus agalactiae, Entrococcus, Listeria monocytogenes and E.Coli. Microscopic examination of stained smears of the large number of Lactobacillus and pathogenic bacteria showed reduced. The prevalence of abnormal vaginal discharge, history of drug use means of preventing pregnancy and douching, respectively, 61%, 55%, 42% and 13% respectively. Significant difference was observed between the use and non-use of IUD in women with bacterial vaginosis infection.Conclusion: Our findings indicated the Inhibitory effect of Lactobacillus rhamnosus on the pathogenic bacteria that cause bacterial vaginosis. The results of this study confirm the hypothesis of inhibit of pathogens growth that cause bacterial vaginosis supported by probiotics and can have beneficial effects in the prevention and treatment of bacterial vaginosis.
Sethi G. - One of the best experts on this subject based on the ideXlab platform.
-
Novel synthetic biscoumarins target tumor necrosis factor-α in hepatocellular carcinoma in vitro and in vivo
'American Society for Biochemistry & Molecular Biology (ASBMB)', 2014Co-Authors: Kumar Keerthy H., Mohan C. D., Siveen K. S., Fuchs J. E., Rangappa S., Sundaram M. S., Li F., Girish K. S., Sethi G.Abstract:TNF is a pleotropic cytokine known to be involved in the progression of several pro-inflammatory disorders. Many therapeutic agents have been designed to counteract the effect of TNF in rheumatoid arthritis as well as a number of cancers. In the present study we have synthesized and evaluated the anti-cancer activity of novel biscoumarins in vitro and in vivo. Among new compounds, BIHC was found to be the most cytotoxic agent against the HepG2 cell line while exhibiting less toxicity toward normal hepatocytes. Furthermore, BIHC inhibited the proliferation of various hepatocellular carcinoma (HCC) cells in a doseand time-dependent manner. Subsequently, using in silico target prediction, BIHC was predicted as a TNF blocker. Experimental validation was able to confirm this hypothesis, where BIHC could significantly inhibit the recombinant mouse TNF-binding to its antibody with an IC50 of 16.5 μM. Furthermore, in silico docking suggested a binding mode of BIHC similar to a ligand known to disrupt the native, trimeric structure of TNF, and also validated with molecular dynamics simulations. Moreover, we have demonstrated the down-regulation of p65 phos-phorylation and other NF-KB-regulated gene products upon BIHC treatment, and on the phenotypic level the compound shows inhibition of CXCLF2-induced invasion of HepG2 cells. Also, we demonstrate that BIHC inhibits infiltration of macrophages to the peritoneal cavity and suppresses the activity of TNF-α in vivo in mice primed with Thioglycollate Broth and lipopolysaccharide. We comprehensively validated the TNF-α inhibitory efficacy of BIHC in an inflammatory bowel disease mice model. © 2014 by I he American bociety tor Biochemistry and Molecular Biology, Inc
-
Novel Synthetic Biscoumarins Target Tumor Necrosis Factor- in Hepatocellular Carcinoma in Vitro and in Vivo*
'American Society for Biochemistry & Molecular Biology (ASBMB)', 2014Co-Authors: Kumar Keerthy H., Rangappa S., Li F., Sethi G., Mohan C.d., Siveen K.s., Fuchs J.e., Sundaram M.s., Girish K.s., . BasappaaAbstract:TNF is a pleotropic cytokine known to be involved in the progression of several pro-inflammatory disorders. Many therapeutic agents have been designed to counteract the effect of TNF in rheumatoid arthritis as well as a number of cancers. In the present study we have synthesized and evaluated the anti-cancer activity of novel biscoumarins in vitro and in vivo. Among new compounds, BIHC was found to be the most cytotoxic agent against the HepG2 cell line while exhibiting less toxicity toward normal hepatocytes. Furthermore, BIHC inhibited the proliferation of various hepatocellular carcinoma (HCC) cells in a doseand time-dependent manner. Subsequently, using in silico target prediction, BIHC was predicted as a TNF blocker. Experimental validation was able to confirm this hypothesis, where BIHC could significantly inhibit the recombinant mouse TNF-binding to its antibody with an IC50 of 16.5 μM. Furthermore, in silico docking suggested a binding mode of BIHC similar to a ligand known to disrupt the native, trimeric structure of TNF, and also validated with molecular dynamics simulations. Moreover, we have demonstrated the down-regulation of p65 phos-phorylation and other NF-KB-regulated gene products upon BIHC treatment, and on the phenotypic level the compound shows inhibition of CXCLF2-induced invasion of HepG2 cells. Also, we demonstrate that BIHC inhibits infiltration of macrophages to the peritoneal cavity and suppresses the activity of TNF-α in vivo in mice primed with Thioglycollate Broth and lipopolysaccharide. We comprehensively validated the TNF-α inhibitory efficacy of BIHC in an inflammatory bowel disease mice model. © 2014 by I he American bociety tor Biochemistry and Molecular Biology, Inc
Daniele L Dallavecchia - One of the best experts on this subject based on the ideXlab platform.
-
efficacy of uv c ray sterilization of calliphora vicina diptera calliphoridae eggs for use in maggot debridement therapy
Journal of Medical Entomology, 2019Co-Authors: Daniele L Dallavecchia, Elisabete Ricardo, Valeria Magalhaes Aguiar, Alexandre Sousa Da Silva, Acacio Goncalves RodriguesAbstract:: Maggot debridement therapy (MDT) is a simple wound debridement technique. It is a natural treatment licensed by the Food and Drug Administration (FDA) and is increasingly used in the United States and in Europe. This treatment is safe when the larvae originate from laboratory stocks of eggs that have been sterilized. In this study, a simple, inexpensive microbe decontamination technique is described. It yields eggs that are free of chemical residues and are easy to handle, meeting the growing demand for medicinal larvae in hospitals or medical centers. Three treatments (T1, T2, T3) involving 3, 6, and 12 min of exposure to ultraviolet (UV-C) rays, respectively, were compared. Egg sterility was evaluated by culture in Thioglycollate Broth, incubated at 32°C ± 2.5°C under aerobic conditions for up to 14 d. The UV-C radiation sterilization process obtained satisfactory results after 12 min exposure (treatment 3). Larval viability was 57%, pupal viability was 54%, and 54% of the adults emerged. The sex ratio was 50%, within the expected values. There were no morphological abnormalities associated to the UV-C treatment in the flies. In conclusion sterilization by UV-C rays is indicated to obtain sterile larvae destined for MDT.