The Experts below are selected from a list of 8553 Experts worldwide ranked by ideXlab platform
Jan Kopecky - One of the best experts on this subject based on the ideXlab platform.
-
Ixodes ricinus tick saliva modulates Tick-Borne Encephalitis Virus infection of dendritic cells.
Microbes and Infection, 2010Co-Authors: Anna Fialová, Zdeněk Cimburek, Giandomenica Iezzi, Jan KopeckyAbstract:Tick-Borne Encephalitis Virus is an important human pathogen, naturally delivered into host skin via a tick bite. To examine the effects of the Virus on dendritic cell biology, we cultured dendritic cells with two Tick-Borne Encephalitis Virus strains of different virulence in the presence of Ixodes ricinus tick saliva. Tick saliva treatment increased proportion of Virus-infected cells, led to a decrease in Virus-induced TNF-α and IL-6 production and to reduced Virus-induced apoptosis. Our data indicate that tick saliva modulate Virus-mediated alterations in dendritic cells, thus probably being involved in the early infection process in the host.
-
Rapid subtyping of Tick-Borne Encephalitis Virus isolates using multiplex RT-PCR.
Journal of Virological Methods, 2007Co-Authors: Hana Šťastná, Irina Golovljova, Jan Kopecky, Libor GrubhofferAbstract:Tick-Borne Encephalitis Virus, an emerging pathogen in several countries in Europe and Asia, has been divided into three subtypes (European, Siberian and Far Eastern). These subtypes are associated with different severities of the disease. For that reason, early determination of the subtype in a clinical sample or in ticks removed from a patient in areas of co-circulation of two or three subtypes is of high importance. The development of a simple method of multiplex RT-PCR for rapid and easy subtyping of Tick-Borne Encephalitis Virus isolates is reported to fill this requirement. The method is based on the unique combination of oligonucleotide primers hybridizing with subtype-specific "signature" positions of the sequence encoding the viral envelope protein. The developed multiplex RT-PCR also appears to be a useful method in studies focused on the molecular-epidemiology of Tick-Borne Encephalitis Virus.
-
Rapid subtyping of Tick-Borne Encephalitis Virus isolates using multiplex RT-PCR.
Journal of Virological Methods, 2007Co-Authors: Hana Šťastná, Irina Golovljova, Jan Kopecky, Libor GrubhofferAbstract:Tick-Borne Encephalitis Virus, an emerging pathogen in several countries in Europe and Asia, has been divided into three subtypes (European, Siberian and Far Eastern). These subtypes are associated with different severities of the disease. For that reason, early determination of the subtype in a clinical sample or in ticks removed from a patient in areas of co-circulation of two or three subtypes is of high importance. The development of a simple method of multiplex RT-PCR for rapid and easy subtyping of Tick-Borne Encephalitis Virus isolates is reported to fill this requirement. The method is based on the unique combination of oligonucleotide primers hybridizing with subtype-specific "signature" positions of the sequence encoding the viral envelope protein. The developed multiplex RT-PCR also appears to be a useful method in studies focused on the molecular-epidemiology of Tick-Borne Encephalitis Virus.
-
Differences in maturation of Tick-Borne Encephalitis Virus in mammalian and tick cell line.
Intervirology, 2006Co-Authors: Filip Šenigl, Libor Grubhoffer, Jan KopeckyAbstract:Objective: The maturation process of Tick-Borne Encephalitis Virus (TBEV) in the tick RA-257 and porcine PS cells was studied by transmission electron microscopy and the E and NS1 p
Magnus Johansson - One of the best experts on this subject based on the ideXlab platform.
-
The role of the poly(A) tract in the replication and virulence of Tick-Borne Encephalitis Virus
Scientific Reports, 2016Co-Authors: Naveed Asghar, Wessam Melik, Emma Nilsson, Anna K. Överby, Yi Ping Lee, Andrea Kröger, Richard Lindqvist, Magnus JohanssonAbstract:The Tick-Borne Encephalitis Virus (TBEV) is a flaviVirus transmitted to humans, usually via tick bites. The Virus causes Tick-Borne Encephalitis (TBE) in humans, and symptoms range from mild flu-li ...
-
Revisiting Recombination Signal in the Tick-Borne Encephalitis Virus: A Simulation Approach.
PLOS ONE, 2016Co-Authors: Yann J. K. Bertrand, Magnus Johansson, Peter NorbergAbstract:The hypothesis of wide spread reticulate evolution in Tick-Borne Encephalitis Virus (TBEV) has recently gained momentum with several publications describing past recombination events involving vari ...
-
Tick-Borne Encephalitis Virus sequenced directly from questing and blood-feeding ticks reveals quasispecies variance
PLoS ONE, 2014Co-Authors: Naveed Asghar, Wessam Melik, Pontus Lindblom, Åshild Kristine Andreassen, Anna K. Överby, Richard Lindqvist, Mats Haglund, Pia Forsberg, Per-eric Lindgren, Magnus JohanssonAbstract:The increased distribution of the Tick-Borne Encephalitis Virus (TBEV) in Scandinavia highlights the importance of characterizing novel sequences within the natural foci. In this study, two TBEV st ...
-
Genomic Sequencing of Tick-Borne Encephalitis Virus frin Questing and Blood-Feeding Ixodes ricinus
2013Co-Authors: Naveed Asghar, Melik Wessam, Pontus Lindblom, Per-erik Lindgren, Åshild Kristine Andreassen, Magnus JohanssonAbstract:Genomic Sequencing of Tick-Borne Encephalitis Virus frin Questing and Blood-Feeding Ixodes ricinus
-
Novel PDZ dependent cell associations of the NS5 proteins of Tick-Borne Encephalitis Virus and West-Nile Virus
2012Co-Authors: Ellen Ellencrona, Wessam Melik, Magnus JohanssonAbstract:Novel PDZ dependent cell associations of the NS5 proteins of Tick-Borne Encephalitis Virus and West-Nile Virus
Jacek Dutkiewicz - One of the best experts on this subject based on the ideXlab platform.
-
prevalence of tick borne Encephalitis Virus in ixodes ricinus and dermacentor reticulatus ticks collected from the lublin region eastern poland
Ticks and Tick-borne Diseases, 2011Co-Authors: Angelina Wojcikfatla, Jacek Zwolinski, Violetta Zając, Ewa Cisak, Jacek DutkiewiczAbstract:Abstract A total of 875 nymphal and adult Ixodes ricinus ticks and 148 adult Dermacentor reticulatus ticks were collected by flagging lower vegetation in the Lublin region (eastern Poland) and examined for the presence of RNA of Tick-Borne Encephalitis Virus (TBEV) by nested RT-PCR. The minimum infection rate of I. ricinus ticks with TBEV amounted to 1.6% while the infection rate of D. reticulatus ticks was 10.8%. The results suggest that D. reticulatus may be a potential vector of TBEV in Central Europe.
Andrey V. Timofeev - One of the best experts on this subject based on the ideXlab platform.
-
Nonstructural protein 1 of Tick-Borne Encephalitis Virus activates the expression of immunoproteasome subunits
Molecular Biology, 2016Co-Authors: Y. V. Kuzmenko, Galina G. Karganova, Andrey V. Timofeev, E. S. Starodubova, V. L. KarpovAbstract:The interaction of viral proteins with host cell components plays an important role in antiviral immune response. One of the key steps of antiviral defense is the formation of immunoproteasomes. The effect of nonstructural protein 1 (NS1) of Tick-Borne Encephalitis Virus on the immunoproteasome formation was studied. It was shown that cell expression of NS1 does not reduce the efficacy of the immunoproteasome generation in response to interferon-γ stimulation and even increases the content of the immunoproteasome subunits without the interferon-γ treatment. Thus, NS1 of Tick-Borne Encephalitis Virus activates, rather than blocks the mechanisms of immune defense in the cell.
-
Synthetic fragments of the NS1 protein of the Tick-Borne Encephalitis Virus exhibiting a protective effect
Russian Journal of Bioorganic Chemistry, 2007Co-Authors: T. D. Volkova, M. F. Vorovich, Andrey V. Timofeev, D. O. Koroev, M. A. Titova, M. B. Oboznaya, M. P. Filatova, S. V. Ozherelkov, O. M. VolpinaAbstract:Potentially immunoactive regions of the NS1 nonstructural protein of the Tick-Borne Encephalitis Virus that can stimulate the antibody formation in vivo and protect animals from this disease were chosen on the basis of theoretical calculations. Eleven 16-to 27-aa peptides containing the chosen regions were synthesized. The ability of the free peptides (without any high-molecular-mass carrier) to stimulate the production of antipeptide antibodies in mice of three lines and ensure the formation of protective immunity was studied. Most of these peptides were shown to exhibit the immunogenic activity in a free state. Five fragments that can protect mice from the infection by a lethal dose of Tick-Borne Encephalitis Virus were found.
-
Vaccinia Virus recombinant expressing gene of Tick-Borne Encephalitis Virus non-structural NS1 protein elicits protective activity in mice.
Immunology letters, 2003Co-Authors: M V Khoretonenko, Mikhail F. Vorovitch, N V Ovsyannikova, J R Stephenson, Anatoly D. Altstein, Andrey V. Timofeev, L. G. Zakharova, G. V. Pashvykina, Alexander M ShneiderAbstract:A vaccinia Virus recombinant containing the non-structural Tick-Borne Encephalitis Virus (TBEV) NS1 gene was developed. The recombinant expressed native dimeric form of the NS1 protein in infected cells and protected mice against lethal infection with TBEV.
-
Synthesis, Immunogenicity, and Antigenicity of the Glycoprotein E Fragments of the Tick-Borne Encephalitis Virus
Russian Journal of Bioorganic Chemistry, 2001Co-Authors: T. D. Volkova, O. M. Vol'pina, M. F. Vorovich, Maxim N. Zhmak, V. T. Ivanov, Andrey V. TimofeevAbstract:Six peptide fragments of the envelope protein E of the Tick-Borne Encephalitis Virus involving the predicted T-helper epitopes were synthesized. Their ability to induce antibodies without conjugation with any high-molecular-mass carrier was studied in mice of three lines. Five of six synthesized peptides exhibited immunogenic properties, which differed in dependence on the haplotype of immunized mice. The peptide binding to the antiviral antibodies was studied, and two peptides were revealed that demonstrated a high ability to recognize the viral antibodies in the horse and human sera. These peptides are promising for the development of diagnostic agents for the Tick-Borne Encephalitis Virus.
-
Synthesis, immunogenicity and antigenic characteristics of the glycoprotein E fragments from the Tick-Borne Encephalitis Virus
Russian Journal of Bioorganic Chemistry, 2001Co-Authors: T. D. Volkova, M. F. Vorovich, Maxim N. Zhmak, V. T. Ivanov, Olga M. Volpina, Andrey V. TimofeevAbstract:Six peptide fragments of the envelope protein E of the Tick-Borne Encephalitis Virus involving the predicted T-helper epitopes were synthesized. Their ability to induce antibodies without conjugation with any high-molecular-mass carrier was studied in mice of three lines. Five of six synthesized peptides exhibited immunogenic properties, which differed in dependence on the haplotype of immunized mice. The peptide binding to the antiviral antibodies was studied, and two peptides were revealed that demonstrated a high ability to recognize the viral antibodies in the horse and human sera. These peptides are promising for the development of diagnostic agents for the Tick-Borne Encephalitis Virus.
Libor Grubhoffer - One of the best experts on this subject based on the ideXlab platform.
-
Rapid subtyping of Tick-Borne Encephalitis Virus isolates using multiplex RT-PCR.
Journal of Virological Methods, 2007Co-Authors: Hana Šťastná, Irina Golovljova, Jan Kopecky, Libor GrubhofferAbstract:Tick-Borne Encephalitis Virus, an emerging pathogen in several countries in Europe and Asia, has been divided into three subtypes (European, Siberian and Far Eastern). These subtypes are associated with different severities of the disease. For that reason, early determination of the subtype in a clinical sample or in ticks removed from a patient in areas of co-circulation of two or three subtypes is of high importance. The development of a simple method of multiplex RT-PCR for rapid and easy subtyping of Tick-Borne Encephalitis Virus isolates is reported to fill this requirement. The method is based on the unique combination of oligonucleotide primers hybridizing with subtype-specific "signature" positions of the sequence encoding the viral envelope protein. The developed multiplex RT-PCR also appears to be a useful method in studies focused on the molecular-epidemiology of Tick-Borne Encephalitis Virus.
-
Rapid subtyping of Tick-Borne Encephalitis Virus isolates using multiplex RT-PCR.
Journal of Virological Methods, 2007Co-Authors: Hana Šťastná, Irina Golovljova, Jan Kopecky, Libor GrubhofferAbstract:Tick-Borne Encephalitis Virus, an emerging pathogen in several countries in Europe and Asia, has been divided into three subtypes (European, Siberian and Far Eastern). These subtypes are associated with different severities of the disease. For that reason, early determination of the subtype in a clinical sample or in ticks removed from a patient in areas of co-circulation of two or three subtypes is of high importance. The development of a simple method of multiplex RT-PCR for rapid and easy subtyping of Tick-Borne Encephalitis Virus isolates is reported to fill this requirement. The method is based on the unique combination of oligonucleotide primers hybridizing with subtype-specific "signature" positions of the sequence encoding the viral envelope protein. The developed multiplex RT-PCR also appears to be a useful method in studies focused on the molecular-epidemiology of Tick-Borne Encephalitis Virus.
-
Differences in maturation of Tick-Borne Encephalitis Virus in mammalian and tick cell line.
Intervirology, 2006Co-Authors: Filip Šenigl, Libor Grubhoffer, Jan KopeckyAbstract:Objective: The maturation process of Tick-Borne Encephalitis Virus (TBEV) in the tick RA-257 and porcine PS cells was studied by transmission electron microscopy and the E and NS1 p
