The Experts below are selected from a list of 707997 Experts worldwide ranked by ideXlab platform
Jennifer Dien Bard - One of the best experts on this subject based on the ideXlab platform.
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back to the basics biochemical testing for pathogen identification in the era of matrix assisted laser desorption ionization time of flight Mass Spectrometry maldi tof ms
Journal of Clinical Microbiology, 2019Co-Authors: Utsav Pandey, Samia N Naccache, Jennifer Dien BardAbstract:Inarguably, matrix-assisted laser desorption ionization–time of flight Mass Spectrometry (MALDI-TOF MS) has revolutionized the pathogen identification process in the clinical microbiology laboratory. However, identification of closely related species of organisms using MALDI-TOF MS remains
Xiang Zhang - One of the best experts on this subject based on the ideXlab platform.
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disco distance and spectrum correlation optimization alignment for two dimensional gas chromatography time of flight Mass Spectrometry based metabolomics
Analytical Chemistry, 2010Co-Authors: Bing Wang, Aiqin Fang, John Heim, Bogdan Bogdanov, Scott Pugh, Mark Libardoni, Xiang ZhangAbstract:A novel peak alignment algorithm using a distance and spectrum correlation optimization (DISCO) method has been developed for two-dimensional gas chromatography Time-of-Flight Mass Spectrometry (GC...
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comprehensive two dimensional gas chromatography time of flight Mass Spectrometry peak sorting algorithm
Journal of Chromatography A, 2008Co-Authors: Cheolhwan Oh, Xiaodong Huang, Fred E Regnier, Charles Buck, Xiang ZhangAbstract:Abstract We report a novel peak sorting method for the two-dimensional gas chromatography/Time-of-Flight Mass Spectrometry (GC × GC/TOF-MS) system. The objective of peak sorting is to recognize peaks from the same metabolite occurring in different samples from thousands of peaks detected in the analytical procedure. The developed algorithm is based on the fact that the chromatographic peaks for a given analyte have similar retention times in all of the chromatograms. Raw instrument data are first processed by ChromaTOF (Leco) software to provide the peak tables. Our algorithm achieves peak sorting by utilizing the first- and second-dimension retention times in the peak tables and the Mass spectra generated during the process of electron impact ionization. The algorithm searches the peak tables for the peaks generated by the same type of metabolite using several search criteria. Our software also includes options to eliminate non-target peaks from the sorting results, e.g., peaks of contaminants. The developed software package has been tested using a mixture of standard metabolites and another mixture of standard metabolites spiked into human serum. Manual validation demonstrates high accuracy of peak sorting with this algorithm.
M W F Nielen - One of the best experts on this subject based on the ideXlab platform.
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Generic sample preparation combined with high-resolution liquid chromatography–Time-of-Flight Mass Spectrometry for unification of urine screening in doping-control laboratories
Analytical and Bioanalytical Chemistry, 2010Co-Authors: R. J. B. Peters, A A M Stolker, J E Oosterink, C. Georgakopoulos, M W F NielenAbstract:A unification of doping-control screening procedures of prohibited small molecule substances—including stimulants, narcotics, steroids, β2-agonists and diuretics—is highly urgent in order to free resources for new classes such as banned proteins. Conceptually this may be achieved by the use of a combination of one gas chromatography–Time-of-Flight Mass Spectrometry method and one liquid chromatography–Time-of-Flight Mass Spectrometry method. In this work a quantitative screening method using high-resolution liquid chromatography in combination with accurate-Mass Time-of-Flight Mass Spectrometry was developed and validated for determination of glucocorticosteroids, β2-agonists, thiazide diuretics, and narcotics and stimulants in urine. To enable the simultaneous isolation of all the compounds of interest and the necessary purification of the resulting extracts, a generic extraction and hydrolysis procedure was combined with a solid-phase extraction modified for these groups of compounds. All 56 compounds are determined using positive electrospray ionisation with the exception of the thiazide diuretics for which the best sensitivity was obtained by using negative electrospray ionisation. The results show that, with the exception of clenhexyl, procaterol, and reproterol, all compounds can be detected below the respective minimum required performance level and the results for linearity, repeatability, within-lab reproducibility, and accuracy show that the method can be used for quantitative screening. If qualitative screening is sufficient the instrumental analysis may be limited to positive ionisation, because all analytes including the thiazides can be detected at the respective minimum required levels in the positive mode. The results show that the application of accurate-Mass Time-of-Flight Mass Spectrometry in combination with generic extraction and purification procedures is suitable for unification and expansion of the window of screening methods of doping laboratories. Moreover, the full-scan accurate-Mass data sets obtained still allow retrospective examination for emerging doping agents, without re-analyzing the samples.
Cecilia Jernberg - One of the best experts on this subject based on the ideXlab platform.
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rapid subtyping of yersinia enterocolitica by matrix assisted laser desorption ionization time of flight Mass Spectrometry maldi tof ms for diagnostics and surveillance
Journal of Clinical Microbiology, 2013Co-Authors: Kristina Rizzardi, Tara Wahab, Cecilia JernbergAbstract:ABSTRACT In this study, an alternative to the current traditional bioserotyping techniques was developed for subtyping Y. enterocolitica using matrix-assisted laser desorption ionization–time of flight Mass Spectrometry (MALDI-TOF MS). The most common pathogenic bioserotypes could easily be distinguished using only a few bioserotype-specific biomarkers. However, biochemical methods should still be used to distinguish biotype 1A from 1B.
Sara E Cosgrove - One of the best experts on this subject based on the ideXlab platform.
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can matrix assisted laser desorption ionization time of flight Mass Spectrometry maldi tof enhance antimicrobial stewardship efforts in the acute care setting
Infection Control and Hospital Epidemiology, 2013Co-Authors: Pranita D Tamma, Kennard Tan, Veronique Nussenblatt, Alison E Turnbull, Karen C Carroll, Sara E CosgroveAbstract:We evaluated 222 hospitalized patients whose clinical isolates were tested using standard methods and matrix-assisted laser desorption ionization Time-of-Flight Mass Spectrometry (MALDI-TOF). MALDI-TOF could have reduced time to appropriate therapy for 28.8% and 44.6% patients based on the treating physician's choices and stewardship team recommendations, respectively. Clinicians should be aware of scenarios in which MALDI-TOF can optimize antibiotic therapy.