The Experts below are selected from a list of 665964 Experts worldwide ranked by ideXlab platform
Richard M. Caprioli - One of the best experts on this subject based on the ideXlab platform.
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processing maldi mass spectra to improve mass spectral direct Tissue Analysis
International Journal of Mass Spectrometry, 2007Co-Authors: Jeremy L Norris, Dale S Cornett, James A Mobley, Malin Andersson, Erin H Seeley, Pierre Chaurand, Richard M. CaprioliAbstract:Profiling and imaging biological specimens using MALDI mass spectrometry has significant potential to contribute to our understanding and diagnosis of disease. The technique is efficient and high-throughput providing a wealth of data about the biological state of the sample from a very simple and direct experiment. However, in order for these techniques to be put to use for clinical purposes, the approaches used to process and analyze the data must improve. This study examines some of the existing tools to baseline subtract, normalize, align, and remove spectral noise for MALDI data, comparing the advantages of each. A preferred workflow is presented that can be easily implemented for data in ASCII format. The advantages of using such an approach are discussed for both molecular profiling and imaging mass spectrometry.
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Direct Tissue Analysis by Matrix-Assisted Laser Desorption Ionization Mass Spectrometry: Application to Kidney Biology
Seminars in Nephrology, 2007Co-Authors: Kristen D. Herring, Stacey R. Oppenheimer, Richard M. CaprioliAbstract:Direct Tissue Analysis using matrix-assisted laser desorption ionization mass spectrometry (MALDI MS) provides in situ molecular Analysis of a wide variety of biological molecules including xenobiotics. This technology allows measurement of these species in their native biological environment without the use of target-specific reagents such as antibodies. It can be used to profile discrete cellular regions and obtain region-specific images, providing information on the relative abundance and spatial distribution of proteins, peptides, lipids, and drugs. In this article, we report the sample preparation, MS data acquisition and Analysis, and protein identification methodologies used in our laboratory for profiling/imaging MS and how this has been applied to kidney disease and toxicity.
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direct Tissue Analysis using matrix assisted laser desorption ionization mass spectrometry practical aspects of sample preparation
Journal of Mass Spectrometry, 2003Co-Authors: Sarah A. Schwartz, Michelle L. Reyzer, Richard M. CaprioliAbstract:Practical guidelines for the preparation of Tissue sections for direct Analysis by matrix-assisted laser desorption/ionization (MALDI) mass spectrometry are presented. Techniques for proper sample handling including Tissue storage, sectioning and mounting are described. Emphasis is placed on optimizing matrix parameters such as the type of matrix molecule used, matrix concentration, and solvent composition. Several different techniques for matrix application are illustrated. Optimal instrument parameters and the necessity for advanced data Analysis approaches with regards to direct Tissue Analysis are also discussed.
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direct Tissue Analysis using matrix assisted laser desorption ionization mass spectrometry practical aspects of sample preparation
Journal of Mass Spectrometry, 2003Co-Authors: Sarah A. Schwartz, Michelle L. Reyzer, Richard M. CaprioliAbstract:Practical guidelines for the preparation of Tissue sections for direct Analysis by matrix-assisted laser desorption/ionization (MALDI) mass spectrometry are presented. Techniques for proper sample handling including Tissue storage, sectioning and mounting are described. Emphasis is placed on optimizing matrix parameters such as the type of matrix molecule used, matrix concentration, and solvent composition. Several different techniques for matrix application are illustrated. Optimal instrument parameters and the necessity for advanced data Analysis approaches with regards to direct Tissue Analysis are also discussed. Copyright © 2003 John Wiley & Sons, Ltd.
Sarah A. Schwartz - One of the best experts on this subject based on the ideXlab platform.
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direct Tissue Analysis using matrix assisted laser desorption ionization mass spectrometry practical aspects of sample preparation
Journal of Mass Spectrometry, 2003Co-Authors: Sarah A. Schwartz, Michelle L. Reyzer, Richard M. CaprioliAbstract:Practical guidelines for the preparation of Tissue sections for direct Analysis by matrix-assisted laser desorption/ionization (MALDI) mass spectrometry are presented. Techniques for proper sample handling including Tissue storage, sectioning and mounting are described. Emphasis is placed on optimizing matrix parameters such as the type of matrix molecule used, matrix concentration, and solvent composition. Several different techniques for matrix application are illustrated. Optimal instrument parameters and the necessity for advanced data Analysis approaches with regards to direct Tissue Analysis are also discussed.
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direct Tissue Analysis using matrix assisted laser desorption ionization mass spectrometry practical aspects of sample preparation
Journal of Mass Spectrometry, 2003Co-Authors: Sarah A. Schwartz, Michelle L. Reyzer, Richard M. CaprioliAbstract:Practical guidelines for the preparation of Tissue sections for direct Analysis by matrix-assisted laser desorption/ionization (MALDI) mass spectrometry are presented. Techniques for proper sample handling including Tissue storage, sectioning and mounting are described. Emphasis is placed on optimizing matrix parameters such as the type of matrix molecule used, matrix concentration, and solvent composition. Several different techniques for matrix application are illustrated. Optimal instrument parameters and the necessity for advanced data Analysis approaches with regards to direct Tissue Analysis are also discussed. Copyright © 2003 John Wiley & Sons, Ltd.
Heinz Hofler - One of the best experts on this subject based on the ideXlab platform.
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direct molecular Tissue Analysis by maldi imaging mass spectrometry in the field of gastrointestinal disease
Gastroenterology, 2012Co-Authors: Benjamin Balluff, Sandra Rauser, Heinz Hofler, Matthias P Ebert, Jens T Siveke, Axel WalchAbstract:*Institute of Pathology, Research Unit Analytical Pathology, Helmholtz Zentrum Munchen, German Research Center for Environmental Health, Neuherberg, Germany; ‡ § Department of Medicine II, Klinikum rechts der Isar, Technische Universitat Munchen, Munich, Germany; Department of Medicine II, Universitatsklinikum Mannheim, University of Heidelberg, Mannheim, Germany; and Institute of Pathology, Technische Universitat Munchen, Munich, Germany
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MALDI imaging mass spectrometry for direct Tissue Analysis: Technological advancements and recent applications
Histochemistry and Cell Biology, 2011Co-Authors: Benjamin Balluff, C Schone, Heinz Hofler, Axel K. WalchAbstract:Matrix assisted laser desorption/ionization (MALDI) imaging mass spectrometry (IMS) is a method that allows the investigation of the molecular content of Tissues within its morphological context. Since it is able to measure the distribution of hundreds of analytes at once, while being label free, this method has great potential which has been increasingly recognized in the field of Tissue-based research. In the last few years, MALDI-IMS has been successfully used for the molecular assessment of Tissue samples mainly in biomedical research and also in other scientific fields. The present article will give an update on the application of MALDI-IMS in clinical and preclinical research. It will also give an overview of the multitude of technical advancements of this method in recent years. This includes developments in instrumentation, sample preparation, computational data Analysis and protein identification. It will also highlight a number of emerging fields for application of MALDI-IMS like drug imaging where MALDI-IMS is used for studying the spatial distribution of drugs in Tissues.
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MALDI imaging mass spectrometry for direct Tissue Analysis: A new frontier for molecular histology
Histochemistry and Cell Biology, 2008Co-Authors: Axel K. Walch, Sren Oliver Deininger, Sandra Rauser, Heinz HoflerAbstract:Matrix-assisted laser desorption/ionization (MALDI) imaging mass spectrometry (IMS) is a powerful tool for investigating the distribution of proteins and small molecules within biological systems through the in situ Analysis of Tissue sections. MALDI-IMS can determine the distribution of hundreds of unknown compounds in a single measurement and enables the acquisition of cellular expression profiles while maintaining the cellular and molecular integrity. In recent years, a great many advances in the practice of imaging mass spectrometry have taken place, making the technique more sensitive, robust, and ultimately useful. In this review, we focus on the current state of the art of MALDI-IMS, describe basic technological developments for MALDI-IMS of animal and human Tissues, and discuss some recent applications in basic research and in clinical settings.
Akos Vertes - One of the best experts on this subject based on the ideXlab platform.
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High-throughput cell and Tissue Analysis with enhanced molecular coverage by laser ablation electrospray ionization mass spectrometry using ion mobility separation
2015Co-Authors: Bindesh Shrestha, Akos VertesAbstract:ABSTRACT: Ambient ionization methods, such as laser ablation electrospray ionization (LAESI), facilitate the direct Analysis of unperturbed cells and Tissues in their native states. However, the lack of a separation step in these ionization techniques results in limited molecular coverage due to interferences, ion suppression effects, and the lack of ability to differentiate between structural isomers and isobaric species. In this contribution, LAESI mass spectrometry (MS) coupled with ion mobility separation (IMS) is utilized for the direct Analysis of protein mixtures, megakaryoblast cell pellets, mouse brain sections, and Arabidopsis thaliana leaves. We demonstrate that the collision cross sections of ions generated by LAESI are similar to the ones obtained by ESI. In various applications, LAESI-IMS-MS allows for the high-throughput separation and mass spectrometric detection of biomolecules on the millisecond time scale with enhanced molecular coverage. For example, direct Analysis of mouse brain Tissue without IMS had yielded ∼300 ionic species, whereas with IMS over 1 100 different ions were detected. Differentiating between ions of similar mass-to-charge ratios with dissimilar drift times in complex biological samples removes some systematic distortions in isotope distributio
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high throughput cell and Tissue Analysis with enhanced molecular coverage by laser ablation electrospray ionization mass spectrometry using ion mobility separation
Analytical Chemistry, 2014Co-Authors: Bindesh Shrestha, Akos VertesAbstract:Ambient ionization methods, such as laser ablation electrospray ionization (LAESI), facilitate the direct Analysis of unperturbed cells and Tissues in their native states. However, the lack of a separation step in these ionization techniques results in limited molecular coverage due to interferences, ion suppression effects, and the lack of ability to differentiate between structural isomers and isobaric species. In this contribution, LAESI mass spectrometry (MS) coupled with ion mobility separation (IMS) is utilized for the direct Analysis of protein mixtures, megakaryoblast cell pellets, mouse brain sections, and Arabidopsis thaliana leaves. We demonstrate that the collision cross sections of ions generated by LAESI are similar to the ones obtained by ESI. In various applications, LAESI-IMS-MS allows for the high-throughput separation and mass spectrometric detection of biomolecules on the millisecond time scale with enhanced molecular coverage. For example, direct Analysis of mouse brain Tissue without...
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High-Throughput Cell and Tissue Analysis with Enhanced Molecular Coverage by Laser Ablation Electrospray Ionization Mass Spectrometry Using Ion Mobility Separation
2014Co-Authors: Bindesh Shrestha, Akos VertesAbstract:Ambient ionization methods, such as laser ablation electrospray ionization (LAESI), facilitate the direct Analysis of unperturbed cells and Tissues in their native states. However, the lack of a separation step in these ionization techniques results in limited molecular coverage due to interferences, ion suppression effects, and the lack of ability to differentiate between structural isomers and isobaric species. In this contribution, LAESI mass spectrometry (MS) coupled with ion mobility separation (IMS) is utilized for the direct Analysis of protein mixtures, megakaryoblast cell pellets, mouse brain sections, and Arabidopsis thaliana leaves. We demonstrate that the collision cross sections of ions generated by LAESI are similar to the ones obtained by ESI. In various applications, LAESI-IMS-MS allows for the high-throughput separation and mass spectrometric detection of biomolecules on the millisecond time scale with enhanced molecular coverage. For example, direct Analysis of mouse brain Tissue without IMS had yielded ∼300 ionic species, whereas with IMS over 1 100 different ions were detected. Differentiating between ions of similar mass-to-charge ratios with dissimilar drift times in complex biological samples removes some systematic distortions in isotope distribution patterns and improves the fidelity of molecular identification. Coupling IMS with LAESI-MS also expands the dynamic range by increasing the signal-to-noise ratio due to the separation of isobaric or other interfering ionic species. We have also shown that identification of potential biomarkers by LAESI can be enhanced by using the drift times of individual ions as an additional parameter in supervised orthogonal projections to latent structures discriminant Analysis. Comparative Analysis of drift time versus mass-to-charge ratio plots was performed for similar Tissue samples to pinpoint significant metabolic differences
Axel K. Walch - One of the best experts on this subject based on the ideXlab platform.
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MALDI imaging mass spectrometry for direct Tissue Analysis: Technological advancements and recent applications
Histochemistry and Cell Biology, 2011Co-Authors: Benjamin Balluff, C Schone, Heinz Hofler, Axel K. WalchAbstract:Matrix assisted laser desorption/ionization (MALDI) imaging mass spectrometry (IMS) is a method that allows the investigation of the molecular content of Tissues within its morphological context. Since it is able to measure the distribution of hundreds of analytes at once, while being label free, this method has great potential which has been increasingly recognized in the field of Tissue-based research. In the last few years, MALDI-IMS has been successfully used for the molecular assessment of Tissue samples mainly in biomedical research and also in other scientific fields. The present article will give an update on the application of MALDI-IMS in clinical and preclinical research. It will also give an overview of the multitude of technical advancements of this method in recent years. This includes developments in instrumentation, sample preparation, computational data Analysis and protein identification. It will also highlight a number of emerging fields for application of MALDI-IMS like drug imaging where MALDI-IMS is used for studying the spatial distribution of drugs in Tissues.
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MALDI imaging mass spectrometry for direct Tissue Analysis: A new frontier for molecular histology
Histochemistry and Cell Biology, 2008Co-Authors: Axel K. Walch, Sren Oliver Deininger, Sandra Rauser, Heinz HoflerAbstract:Matrix-assisted laser desorption/ionization (MALDI) imaging mass spectrometry (IMS) is a powerful tool for investigating the distribution of proteins and small molecules within biological systems through the in situ Analysis of Tissue sections. MALDI-IMS can determine the distribution of hundreds of unknown compounds in a single measurement and enables the acquisition of cellular expression profiles while maintaining the cellular and molecular integrity. In recent years, a great many advances in the practice of imaging mass spectrometry have taken place, making the technique more sensitive, robust, and ultimately useful. In this review, we focus on the current state of the art of MALDI-IMS, describe basic technological developments for MALDI-IMS of animal and human Tissues, and discuss some recent applications in basic research and in clinical settings.
