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M. P. Jadhav - One of the best experts on this subject based on the ideXlab platform.

  • Embryo excision and growth evaluation of chickpea (Cicer arietinum) on Tissue Culture Medium
    Journal of Pharmacognosy and Phytochemistry, 2017
    Co-Authors: Pp Behere, B.u. Rathod, N.r. Dattagonde, M. P. Jadhav
    Abstract:

    In present study efforts were made to improve to study the effects of different growth regulators with different concentration on excised embryo from overnight soaked mature seeds of Chickpea. For study of Chickpea, seeds were sterilized and soaked overnight aseptically and then used for explants isolation. For both explants we use the mature Chickpea seeds. These seeds used for isolation of embryonic axis and Cotyladory Node explants. Both explants inoculated on different concentrations of growth regulators. Among those MS media fortified with 3 mg/L BAP shows maximum shoot initiation. The mature embryonic axis explants of cv. Vijay (Phule G-81-1-1) showed maximum growth and development in all types of media for all types growth conditions. The MS media fortified with 0.3 mg/L IBA as growth regulators gave better results for root formation in both types of explants and in both cultivars. In MS media without growth regulators, very poor response had occurred.

  • Development and validation of liquid chromatography-tandem mass spectrometry method for detection and quantification of flunisolide in Tissue Culture Medium.
    Analytical Abstracts, 2013
    Co-Authors: M. P. Jadhav, Yufei Tang, Guenther Hochhaus
    Abstract:

    A robust, sensitive, and selective liquid chromatography-tandem mass spectrometry (LC-MS/MS) method capable of quantifying flunisolide in the Tissue Culture matrix was developed and validated. Both flunisolide and dexamethasone (internal standard) were extracted from Tissue Culture Medium, with 5% fetal bovine serum, 1% nonessential amino acids, and 1% penicillin/streptomycin by simple liquid-liquid extraction. The analytes were chromatographically separated (10 ng/mL for flunisolide) using a C8 column (4.6 mm × 150 mm; 5 µm particle size). The mobile phase was comprised of methanol:water (80:20 v/v). The analytes were separated at baseline within 2.5 min. using a flow rate of 1 mL/min. Mass spectrometry detection was carried out in negative atmospheric pressure chemical ionization mode. The calibration curves for the analyte were linear over the range of 10-200 ng/mL (R2 ≥ 0.9968, n = 6). The inter and intra-batch mean percent accuracy ranged within 97.7-110.6% and the precision range was 3.5-10.4% (% coefficient of variation ≤ 15%). Stability studies revealed that the analyte was stable in the matrix for at least six hours at room temperature and at the end of three successive freeze and thaw cycles. The method reported here is specific for flunisolide quantification was also used in monolayer efflux assay using CaCo2 cell lines, which will eventually aid P-glycoprotein transport studies for flunisolide.

  • Development and Validation of Liquid Chromatography-Tandem Mass Spectrometry Method for Detection and Quantification of Flunisolide in Tissue Culture Medium
    Analytical Letters, 2013
    Co-Authors: M. P. Jadhav, Yufei Tang, Guenther Hochhaus
    Abstract:

    A robust, sensitive, and selective liquid chromatography-tandem mass spectrometry (LC-MS/MS) method capable of quantifying flunisolide in the Tissue Culture matrix was developed and validated. Both flunisolide and dexamethasone (internal standard) were extracted from Tissue Culture Medium, with 5% fetal bovine serum, 1% nonessential amino acids, and 1% penicillin/streptomycin by simple liquid–liquid extraction. The analytes were chromatographically separated (10 ng/mL for flunisolide) using a C8 column (4.6 mm × 150 mm; 5 µm particle size). The mobile phase was comprised of methanol:water (80:20 v/v). The analytes were separated at baseline within 2.5 min. using a flow rate of 1 mL/min. Mass spectrometry detection was carried out in negative atmospheric pressure chemical ionization mode. The calibration curves for the analyte were linear over the range of 10–200 ng/mL (R2 ≥ 0.9968, n = 6). The inter and intra-batch mean percent accuracy ranged within 97.7–110.6% and the precision range was 3.5–10.4% (% co...

Guenther Hochhaus - One of the best experts on this subject based on the ideXlab platform.

  • Development and validation of liquid chromatography-tandem mass spectrometry method for detection and quantification of flunisolide in Tissue Culture Medium.
    Analytical Abstracts, 2013
    Co-Authors: M. P. Jadhav, Yufei Tang, Guenther Hochhaus
    Abstract:

    A robust, sensitive, and selective liquid chromatography-tandem mass spectrometry (LC-MS/MS) method capable of quantifying flunisolide in the Tissue Culture matrix was developed and validated. Both flunisolide and dexamethasone (internal standard) were extracted from Tissue Culture Medium, with 5% fetal bovine serum, 1% nonessential amino acids, and 1% penicillin/streptomycin by simple liquid-liquid extraction. The analytes were chromatographically separated (10 ng/mL for flunisolide) using a C8 column (4.6 mm × 150 mm; 5 µm particle size). The mobile phase was comprised of methanol:water (80:20 v/v). The analytes were separated at baseline within 2.5 min. using a flow rate of 1 mL/min. Mass spectrometry detection was carried out in negative atmospheric pressure chemical ionization mode. The calibration curves for the analyte were linear over the range of 10-200 ng/mL (R2 ≥ 0.9968, n = 6). The inter and intra-batch mean percent accuracy ranged within 97.7-110.6% and the precision range was 3.5-10.4% (% coefficient of variation ≤ 15%). Stability studies revealed that the analyte was stable in the matrix for at least six hours at room temperature and at the end of three successive freeze and thaw cycles. The method reported here is specific for flunisolide quantification was also used in monolayer efflux assay using CaCo2 cell lines, which will eventually aid P-glycoprotein transport studies for flunisolide.

  • Development and Validation of Liquid Chromatography-Tandem Mass Spectrometry Method for Detection and Quantification of Flunisolide in Tissue Culture Medium
    Analytical Letters, 2013
    Co-Authors: M. P. Jadhav, Yufei Tang, Guenther Hochhaus
    Abstract:

    A robust, sensitive, and selective liquid chromatography-tandem mass spectrometry (LC-MS/MS) method capable of quantifying flunisolide in the Tissue Culture matrix was developed and validated. Both flunisolide and dexamethasone (internal standard) were extracted from Tissue Culture Medium, with 5% fetal bovine serum, 1% nonessential amino acids, and 1% penicillin/streptomycin by simple liquid–liquid extraction. The analytes were chromatographically separated (10 ng/mL for flunisolide) using a C8 column (4.6 mm × 150 mm; 5 µm particle size). The mobile phase was comprised of methanol:water (80:20 v/v). The analytes were separated at baseline within 2.5 min. using a flow rate of 1 mL/min. Mass spectrometry detection was carried out in negative atmospheric pressure chemical ionization mode. The calibration curves for the analyte were linear over the range of 10–200 ng/mL (R2 ≥ 0.9968, n = 6). The inter and intra-batch mean percent accuracy ranged within 97.7–110.6% and the precision range was 3.5–10.4% (% co...

Bagian Reproduksi - One of the best experts on this subject based on the ideXlab platform.

  • TINGKAT PERKEMBANGAN AWAL EMBRIO SAPI IN VITRO MENGGUNAKAN MEDIA TUNGGAL BERBAHAN DASAR Tissue Culture Medium (TCM) 199 Early Bovine Embryonic Development Rate in Vitro Using Single Medium Based on Tissue Culture Medium (TCM) 199
    2013
    Co-Authors: Mohamad Agus Setiadi, Bagian Reproduksi
    Abstract:

    Research on early bovine embryonic development in vitro has been done. Oocytes were collected by aspiration technique from slaughter house ovarian and classified based on the number of cumulus cell layers and homogenous of cytoplasm. Maturation media consisted of Tissue Culture Medium (TCM) 199 supplemented with 10 IU/ml pregnant mare’s serum gonadotropin (PMSG), 10 IU/ml human chorionic gonadotropin (hCG), and 10% fetal bovine serum (FBS). Maturation were done in 5% CO2 incubator 39 C for 24 h. Fertilization was done using two different media namely routine fertilization media and media based on TCM 199 supplemented with BSA and heparin. After fertilization periode, oocytes were denuded and then Cultured in TCM 199 supplemented with essential and nonessential amino acid and 10% FBS for three days. Maturation, fertilization, and Culture were done in 5% CO2 incubator 39 C. Results of the experment revealed that number of mature oocytes (MII) in our system reaches 81.5%. Meanwhile number of embryonic development were higher in routine media compared to media based on TCM 199 (44.4% vs 23.2%). However, there were no signifcant difference in the number 4-8 cells embryo in both media. It is concluded that single media based on TCM 199 could be used for in vitro embryo production. However further research are required on addition comppound to improve fertilization and embryonic development in the media based on TCM 199.

Joon-seok Chae - One of the best experts on this subject based on the ideXlab platform.

  • The Effect of Oral Administration of Tissue Culture Medium Waste of Korean Wild Ginseng on Meat Quality of Broiler Chickens
    Korean Journal of Poultry Science, 2010
    Co-Authors: Jae-won Seol, Jae-hong Park, Joon-seok Chae, Hyung-sub Kang, Kyeong-seon Ryu, Chun-seong Kang, Sang-youel Park
    Abstract:

    E&T Co. Ltd., Nonsan 320-930, KoreaABSTRACT TCM-KWG (Tissue Culture Medium waste after harvest of Korean wild ginseng) (panax ginseng) is left over of Tissue Culture Medium used to grow Korean wild ginseng (KWG). The present study was conducted to investigate the effect of TCM-KWG on meat quality and the possible of application as additives in broiler chickens. A day old broiler chickens randomized in 6 groups (n=60/groups) were administered orally with 2, 4, 8, 16, 32 mL/L TCM-KWG through drinking water with one untreated control group. After administration for five weeks, we analyzed chemical composition and meat quality. Crude ash increased approximately 20% in TCM-KWG treatment groups as compared to control group whereas the concentration of moisture, crude protein and crude fat did not show any significant difference. Crude ash is essential to enhance skeleton formation and physiological function. TCM-KWG treatment gradually decreased the pH value of breast meat whereas it did not change the purge loss and cooking loss. The pH value of meat is important for preservation of meat for longer periods and high durability strength. These results suggest that TCM-KWG treatment may improve the quality of meat and can be apply as food additives in chickens.(Key words : Panax ginseng, TCM-KWG, crude ash, pH value, broiler chicken)To whom correspondence should be addressed : sypark@chonbuk.ac.kr

  • Control of Fowl Typhoid Using Tissue Culture Medium Waste After Harvest of Korean Wild Ginseng (Panax ginseng)
    The Journal of Applied Poultry Research, 2005
    Co-Authors: Devendra H. Shah, Jae-won Seol, Jae-hong Park, Hyung-sub Kang, Kyeong-seon Ryu, Chun-seong Kang, Sang-youel Park, J.-t. Kwon, M.-r. Cho, Joon-seok Chae
    Abstract:

    Abstract Fowl typhoid (FT), caused by Salmonella gallinarum (SG), is responsible for heavy economic losses through morbidity, mortality, and reduced egg production to the poultry industry worldwide. The use of alternative medications to control FT is important in light of emergence of multiple drug-resistant strains of SG and the public's desire to have organically grown poultry. We report the efficient utilization of Tissue Culture Medium waste after harvest of Korean wild ginseng (TCM-KWG) (Panax ginseng) as an alternative medicine in the control of FT. In a series of experiments performed in this study, we found that oral administration of TCM-KWG through drinking water significantly reduced the mortality, organ invasiveness, and fecal shedding of SG in 1-d-old chickens experimentally infected with virulent SG. The results were positively correlated with higher dose and longer duration of pretreatment with TCM-KWG in relation to reduction in mortality caused by FT. The results of this work indicate that the TCM-KWG may be used as a cost-effective and environmentally friendly alternative medicine to control and minimize the losses due to FT in chickens.

Rosana Maria Dos Reis - One of the best experts on this subject based on the ideXlab platform.

  • Supplemented Tissue Culture Medium 199 is a better Medium for in vitro maturation of oocytes from women with polycystic ovary syndrome women than human tubal fluid
    Fertility and Sterility, 2008
    Co-Authors: Carlos Henrique Medeiros De Araújo, D. Nogueira, Maria Cristina Picinato Medeiros De Araújo, Wellington P. Martins, Rui Alberto Ferriani, Rosana Maria Dos Reis
    Abstract:

    Objective To compare oocyte maturation, fertilization and cleavage rates, and embryonic developmental quality after Culture of human immature oocytes from polycystic ovary syndrome (PCOS) patients in human tubal fluid (HTF) or Tissue Culture Medium (TCM) 199. Design Prospective, randomized, controlled trial. Setting University hospital. Patient(s) Thirteen women undergoing 23 in vitro maturation cycles, from whom 119 oocytes were retrieved. Intervention(s) Cumulus-enclosed germinal vesicle–stage oocytes matured in TCM-199-supplemented or HTF-supplemented media. Main Outcome Measure(s) Oocyte maturation and fertilization rates, embryonic developmental quality. Result(s) Significant differences were observed between TCM 199 and HTF regarding maturation rate (82% vs. 56.9%), fertilization rate (70% vs. 39.4%), and embryo quality (81.3% vs. 41.7%). Conclusion(s) Human tubal fluid Medium, although widely used for embryo fertilization and maintenance in IVF techniques, is not an appropriate Medium for the maturation of oocytes obtained from PCOS patients in nonstimulated cycles.