The Experts below are selected from a list of 198 Experts worldwide ranked by ideXlab platform
Patrick J Cozzone - One of the best experts on this subject based on the ideXlab platform.
-
multiparametric optimization of 31p nmr spectroscopic analysis of phospholipids in crude Tissue Extracts 2 line width and spectral resolution
Analytical Chemistry, 2010Co-Authors: Norbert W Lutz, Patrick J CozzoneAbstract:The quality of NMR spectra in general and of spectra to be used for analysis of compound mixtures in particular is essentially defined by two basic parameters: signal-to-noise ratio and spectral resolution. The latter is determined by signal dispersion (chemical shift differences) and line widths. The present study focuses on multiparametric optimization of spectral resolution in (31)P NMR spectra of phospholipids from brain Tissue Extracts. This report presents, for the first time, a systematic and comprehensive study of phospholipid (31)P NMR line widths as a function of four experimental parameters: (i) Extract concentration, (ii) concentration of a chelating agent, (iii) pH of the aqueous component of the solvent system, and (iv) temperature of the NMR measurement. Theoretical underpinnings of observed line width variations (transversal relaxation effects) are briefly discussed. In conjunction with an analogous, concurrently published report on chemical shift effects in the same Tissue Extract system, this multiparametric line width study provides a complete set of methodological guidelines for (i) generating well-defined Tissue Extracts, and (ii) choosing matched and optimized measurement conditions for highly reproducible and well-resolved (31)P NMR spectra of brain phospholipids. This study also offers a comprehensive database and a strategy for rational and efficient optimization of phospholipid spectra from other Tissue Extracts.
-
Multiparametric optimization of (31)P NMR spectroscopic analysis of phospholipids in crude Tissue Extracts. 2. Line width and spectral resolution
Analytical Chemistry, 2010Co-Authors: Norbert W Lutz, Patrick J CozzoneAbstract:The quality of NMR spectra in general and of spectra to be used for analysis of compound mixtures in particular is essentially defined by two basic parameters: signal-to-noise ratio and spectral resolution. The latter is determined by signal dispersion (chemical shift differences) and line widths. The present study focuses on multiparametric optimization of spectral resolution in (31)P NMR spectra of phospholipids from brain Tissue Extracts. This report presents, for the first time, a systematic and comprehensive study of phospholipid (31)P NMR line widths as a function of four experimental parameters: (i) Extract concentration, (ii) concentration of a chelating agent, (iii) pH of the aqueous component of the solvent system, and (iv) temperature of the NMR measurement. Theoretical underpinnings of observed line width variations (transversal relaxation effects) are briefly discussed. In conjunction with an analogous, concurrently published report on chemical shift effects in the same Tissue Extract system, this multiparametric line width study provides a complete set of methodological guidelines for (i) generating well-defined Tissue Extracts, and (ii) choosing matched and optimized measurement conditions for highly reproducible and well-resolved (31)P NMR spectra of brain phospholipids. This study also offers a comprehensive database and a strategy for rational and efficient optimization of phospholipid spectra from other Tissue Extracts.
Norbert W Lutz - One of the best experts on this subject based on the ideXlab platform.
-
multiparametric optimization of 31p nmr spectroscopic analysis of phospholipids in crude Tissue Extracts 2 line width and spectral resolution
Analytical Chemistry, 2010Co-Authors: Norbert W Lutz, Patrick J CozzoneAbstract:The quality of NMR spectra in general and of spectra to be used for analysis of compound mixtures in particular is essentially defined by two basic parameters: signal-to-noise ratio and spectral resolution. The latter is determined by signal dispersion (chemical shift differences) and line widths. The present study focuses on multiparametric optimization of spectral resolution in (31)P NMR spectra of phospholipids from brain Tissue Extracts. This report presents, for the first time, a systematic and comprehensive study of phospholipid (31)P NMR line widths as a function of four experimental parameters: (i) Extract concentration, (ii) concentration of a chelating agent, (iii) pH of the aqueous component of the solvent system, and (iv) temperature of the NMR measurement. Theoretical underpinnings of observed line width variations (transversal relaxation effects) are briefly discussed. In conjunction with an analogous, concurrently published report on chemical shift effects in the same Tissue Extract system, this multiparametric line width study provides a complete set of methodological guidelines for (i) generating well-defined Tissue Extracts, and (ii) choosing matched and optimized measurement conditions for highly reproducible and well-resolved (31)P NMR spectra of brain phospholipids. This study also offers a comprehensive database and a strategy for rational and efficient optimization of phospholipid spectra from other Tissue Extracts.
-
Multiparametric optimization of (31)P NMR spectroscopic analysis of phospholipids in crude Tissue Extracts. 2. Line width and spectral resolution
Analytical Chemistry, 2010Co-Authors: Norbert W Lutz, Patrick J CozzoneAbstract:The quality of NMR spectra in general and of spectra to be used for analysis of compound mixtures in particular is essentially defined by two basic parameters: signal-to-noise ratio and spectral resolution. The latter is determined by signal dispersion (chemical shift differences) and line widths. The present study focuses on multiparametric optimization of spectral resolution in (31)P NMR spectra of phospholipids from brain Tissue Extracts. This report presents, for the first time, a systematic and comprehensive study of phospholipid (31)P NMR line widths as a function of four experimental parameters: (i) Extract concentration, (ii) concentration of a chelating agent, (iii) pH of the aqueous component of the solvent system, and (iv) temperature of the NMR measurement. Theoretical underpinnings of observed line width variations (transversal relaxation effects) are briefly discussed. In conjunction with an analogous, concurrently published report on chemical shift effects in the same Tissue Extract system, this multiparametric line width study provides a complete set of methodological guidelines for (i) generating well-defined Tissue Extracts, and (ii) choosing matched and optimized measurement conditions for highly reproducible and well-resolved (31)P NMR spectra of brain phospholipids. This study also offers a comprehensive database and a strategy for rational and efficient optimization of phospholipid spectra from other Tissue Extracts.
Verónica I. Dumit - One of the best experts on this subject based on the ideXlab platform.
-
Induction of Tyrosine Hydroxylase Gene Expression in Embryonal Carcinoma Stem Cells Using a Natural Tissue-Specific Inducer.
Developmental neurobiology, 2019Co-Authors: Nazila Momendoust, Jamal Moshtaghian, Fariba Esmaeili, Fariba Dehghanian, Verónica I. DumitAbstract:A large number of studies have focused on the generation of dopaminergic neurons from pluripotent cells. Differentiation of stem cells into distinct cell types is influenced by Tissue-specific microenvironment. Since, central nervous system undergoes further development during postnatal life, in the present study neonatal rat brain Tissue Extract (NRBE) was applied to direct the differentiation of embryonal carcinoma stem cell line, P19 into dopaminergic (DA) phenotypes. Additionally, a neuroprotective drug, deprenyl was used alone or in combination with the Extract. Results from morphological, immunofluorescence, and qPCR analyses showed that during a period of one to three weeks, a large percentage of stem cells were differentiated into neural cells. The results also indicated the greater effect of NRBE on the differentiation of the cells into tyrosine hydroxylase-expressing cells. MS analysis of NRBE showed the enrichment of gene ontology terms related to cell differentiation and neurogenesis. Network analysis of the studied genes and some DA markers resulted in the suggestion of potential regulatory candidates such as AVP, ACHE, LHFPL5, and DLK1 genes. In conclusion, NRBE as a natural native inducer was apparently able to simulate the brain microenvironment and support neural differentiation of P19 cells.
S. Gennero - One of the best experts on this subject based on the ideXlab platform.
-
Lung-Tissue Extract as an alternative to serum for surveillance for brucellosis in chamois.
Preventive veterinary medicine, 2000Co-Authors: E Ferroglio, L Rossi, S. GenneroAbstract:Abstract Serological surveys are the most-used way to study diseases in free-ranging wild animals. However, the difficulty in obtaining a sufficient number of suitable serum samples is a major problem. To resolve this problem, we investigated the possibility of using lung-Tissue Extract in place of blood serum for searching for antibodies against Brucella abortus. Antibodies titres against B. abortus was tested from blood serum and lung-Tissue Extract from 112 chamois and 99 cattle. Although in complement-fixation-test, lung-Tissue Extract titres usually were one-to three-fold lower than serum titres, there was a good agreement between serum and lung-Tissue Extract positivity both in chamois and in cattle. The lung-Tissue Extract appears a suitable resource in monitoring brucellosis in chamois.
Ute Schäfer - One of the best experts on this subject based on the ideXlab platform.
-
Embryonic stem cells produce neurotrophins in response to cerebral Tissue Extract: Cell line-dependent differences.
Journal of neuroscience research, 2007Co-Authors: Kristine Bentz, Marek Molcanyi, Peter Riess, Andrea Elbers, Esther Pohl, Agapios Sachinidis, Jürgen Hescheler, Edmund Neugebauer, Ute SchäferAbstract:In the present study, we compare the capacity of two different embryonic stem (ES) cell lines to secrete neurotrophins in response to cerebral Tissue Extract derived from healthy or injured rat brains. The intrinsic capacity of the embryonic cell lines BAC7 (feeder cell-dependent cultivation) to release brain-derived neurotrophic factor (BDNF) or neurotrophin-3 (NT-3) exceeded the release of these factors by CGR8 cells (feeder cell-free growth) by factors of 10 and 4, respectively. Nerve growth factor (NGF) was secreted only by BAC7 cells. Conditioning of cell lines with cerebral Tissue Extract derived from healthy or fluid percussion-injured rat brains resulted in a significant time-dependent increase in BDNF release in both cell lines. The increase in BDNF release by BAC7 cells was more pronounced when cells were incubated with brain Extract derived from injured brain. However, differences in neurotrophin release associated with the origin of brain Extract were at no time statistically significant. Neutrophin-3 and NGF release was inhibited when cell lines were exposed to cerebral Tissue Extract. The magnitude of the response to cerebral Tissue Extract was dependent on the intrinsic capacity of the cell lines to release neurotrophins. Our results clearly demonstrate significant variations in the intrinsic capability of different stem cell lines to produce neurotrophic factors. Furthermore, a significant modulation of neurotrophic factor release was observed following conditioning of cell lines with Tissue Extract derived from rat brains. A significant modulation of neurotrophin release dependent on the source of cerebral Tissue Extract used was not observed.
