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Asav Engin - One of the best experts on this subject based on the ideXlab platform.
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SENSITIVE DETERMINATION OF 3,4-DIHYDROXY-L-PHENYLALANINE BY A CLOUD FUNNEL MUSHROOM (Clitocybe nebularis (Batsch), P. Kumm.) Homogenate-BASED AMPEROMETRIC BIOSENSOR
'Trakya University Journal of Natural Sciences', 2021Co-Authors: Asav EnginAbstract:3,4-dihydroxy-L-phenylalanine (L-DOPA) is one of the precursor molecules for the biosynthesis of neurotransmitters in the brain. Monitoring of L-DOPA levels as a drug or biomolecule in biological fluids is crucial for the treatment of patients suffering from Parkinson's Disease. This study aimed to construct a cloud funnel mushroom (Clitocybe nebularis (Batsch), P. Kumm.) Tissue Homogenate-based biosensor for precise and sensitive detection of L-DOPA in artificial plasma and urine. For this purpose, in the fabrication of the biosensor, Tissue Homogenate of C. nebularis was immobilized into a carbon paste electrode by using graphite, mineral oil, gelatine and glutaraldehyde. The amperometric signals corresponding to 600 s were recorded as response current for each L-DOPA concentration. All amperometric measurements were carried out at.700 mV (versus Ag vertical bar AgCl). The present biosensor successfully detected L-DOPA with a linear dynamic range at 2.5-100 mu M and Limit of Detection (LOD) value as 0.76 mu M, as well as standard deviation as +/- 0.41 mu M and coefficient of variation as 0.82% (n=16). Additionally, the determination of L-DOPA spiked in artificial plasma and urine was carried out successfully. The present work would be the first study that utilized C. nebularis Tissue as a biosensor component.WOS:00070895960001
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Sensitive Determination of 3,4-Dihydroxy-L-Phenylalanine by a Cloud Funnel Mushroom (Clitocybe Nebularis (Batsch), P. Kumm.) Homogenate-Based Amperometric Biosensor
'Trakya University Journal of Natural Sciences', 2021Co-Authors: Asav EnginAbstract:DergiPark: 969982trkjnat3,4-dihidroksifenilalanin (L-DOPA), beyinde nörotransmitter sentezi için öncül moleküllerden biridir. Biyolojik sıvılarda, ilaç veya biyomolekül olarak L-DOPA düzeylerinin izlenmesi, Parkinson hastalığına sahip kişilerin tedavi süreci için önemlidir. Bu çalışmanın amacı, sentetik plazma ve idrar örneklerinde L-DOPA molekülünün doğru ve duyarlı bir tayinine yönelik, bulutlu huni mantarı (Clitocybe nebularis (Batsch), P. Kumm.) doku homejenatı temelli bir biyosensör sistemi geliştirmektir. Bu bağlamda, biyosensör yapımında, C. nebularis doku homojenatı; grafit, mineral yağ, jelatin ve glutaraldehit kullanılarak karbon pasta elektrot içine immobilize edilmişlerdir. 600. saniyeye karşılık gelen amperometrik sinyaller her L-DOPA konsantrasyonu için yanıt akımı olarak kaydedilmiştir. Tüm amperometrik ölçümler ?700 mV (vs Ag|AgCl) potansiyelinde gerçekleştirilmiştir. Geliştirilen biyosensör, L-DOPA molekülünü 2,5-100 µM tayin aralığında ve 0,76 µM tayin limitinin yanı sıra ±0,41 µM standart sapma (n= 16). ve %0,82 varyasyon katsayısı ile saptayabilmiştir. Ayrıca sentetik plazma ve idrar içerisine eklenmiş L-DOPA miktarının da tayini başarı ile gerçekleştirilmiştir. Bu çalışma C. nebularis dokusunun ilk kez bir biyosensör bileşeni olarak kullanıldığı çalışmadır.3,4-dihydroxy-L-phenylalanine (L-DOPA) is one of the precursor molecules for the biosynthesis of neurotransmitters in the brain. Monitoring of L-DOPA levels as a drug or biomolecule in biological fluids is crucial for the treatment of patients suffering from Parkinson’s Disease. This study aimed to construct a cloud funnel mushroom (Clitocybe nebularis (Batsch), P. Kumm.) Tissue Homogenate-based biosensor for precise and sensitive detection of L-DOPA in artificial plasma and urine. For this purpose, in the fabrication of the biosensor, Tissue Homogenate of C. nebularis was immobilized into a carbon paste electrode by using graphite, mineral oil, gelatine and glutaraldehyde. The amperometric signals corresponding to 600 s were recorded as response current for each L-DOPA concentration. All amperometric measurements were carried out at ?700 mV (versus Ag|AgCl). The present biosensor successfully detected L-DOPA with a linear dynamic range at 2.5-100 µM and Limit of Detection (LOD) value as 0.76 µM, as well as standard deviation as ±0.41 µM and coefficient of variation as 0.82% (n=16). Additionally, the determination of L-DOPA spiked in artificial plasma and urine was carried out successfully. The present work would be the first study that utilized C. nebularis Tissue as a biosensor component.
Jarogniew J łuszczki - One of the best experts on this subject based on the ideXlab platform.
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comparison of mouse plasma and brain Tissue Homogenate sample pretreatment methods prior to high performance liquid chromatography for a new 1 2 4 triazole derivative with anticonvulsant activity
Journal of Separation Science, 2015Co-Authors: Jolanta Flieger, Anna Kowalska, Magdalena Pizon, Tomasz Plech, Jarogniew J łuszczkiAbstract:The focus of the study was to develop a bio-analytical assay for a 1,2,4-triazole derivative from plasma and brain Tissue Homogenate samples. The goal was to compare analytical techniques that facilitate high accuracy with simplified sample processing. In this study, commonly used standard protein precipitation and solid-phase extraction methods utilizing C18 and cartridges of Hybrid technology were compared in terms of their ability for sample pretreatment and removal of biological matrices before high-performance liquid chromatography quantification. Fast classical reversed-phase chromatography on a C18 column paired with selective sample preparation using Hybrid solid-phase extraction technology resulted in the most precise bio-analytical determination of the hydrophobic 1,2,4-triazole derivative in both biological samples studied. The obtained recovery values were above 95% with the coefficient of variation lower than 5%.
Jolanta Flieger - One of the best experts on this subject based on the ideXlab platform.
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comparison of mouse plasma and brain Tissue Homogenate sample pretreatment methods prior to high performance liquid chromatography for a new 1 2 4 triazole derivative with anticonvulsant activity
Journal of Separation Science, 2015Co-Authors: Jolanta Flieger, Anna Kowalska, Magdalena Pizon, Tomasz Plech, Jarogniew J łuszczkiAbstract:The focus of the study was to develop a bio-analytical assay for a 1,2,4-triazole derivative from plasma and brain Tissue Homogenate samples. The goal was to compare analytical techniques that facilitate high accuracy with simplified sample processing. In this study, commonly used standard protein precipitation and solid-phase extraction methods utilizing C18 and cartridges of Hybrid technology were compared in terms of their ability for sample pretreatment and removal of biological matrices before high-performance liquid chromatography quantification. Fast classical reversed-phase chromatography on a C18 column paired with selective sample preparation using Hybrid solid-phase extraction technology resulted in the most precise bio-analytical determination of the hydrophobic 1,2,4-triazole derivative in both biological samples studied. The obtained recovery values were above 95% with the coefficient of variation lower than 5%.
Stephen A Wise - One of the best experts on this subject based on the ideXlab platform.
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certification of a frozen mussel Tissue standard reference material srm 1974a for trace organic constituents
Fresenius Journal of Analytical Chemistry, 1997Co-Authors: Michele M Schantz, Melinda J Hays, Katherine S Sharpless, Dianne L. Poster, Rabia Demiralp, Robert R Greenberg, Reenie M. Parris, Barbara J. Porter, Lane C Sander, Stephen A WiseAbstract:NIST SRM 1974a, Organics in Mussel Tissue (Mytilus edulis), has been issued as a frozen Tissue Homogenate with certified mass fractions for 15 polycyclic aromatic hydrocarbons (PAHs), 20 polychlorinated biphenyl (PCB) congeners, and 7 chlorinated pesticides. Noncertified mass fractions are provided for an additional 18 PAHs, 4 PCB congeners, 4 chlorinated pesticides, 28 inorganic constituents, 16 aliphatic hydrocarbons, and methylmercury. The mass fractions for the measured PAHs range from approximately 1 μg/kg to 164 μg/kg dry mass while the mass fractions for the measured PCB congeners range from approximately 3 μg/kg to 150 μg/kg dry mass.
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certification of polychlorinated biphenyl congeners and chlorinated pesticides in a whale blubber standard reference material
Analytical Chemistry, 1995Co-Authors: Michele M Schantz, Barbara J Koster, Susannah B Schiller, Lisa M Oakley, Stephen A WiseAbstract:A Standard Reference Material (SRM) made from whale blubber has been developed for the validation of methods used for the determination of polychlorinated biphenyl (PCB) congeners and chlorinated pesticides. This material, which is a frozen blubber Tissue Homogenate, was analyzed using three different analytical techniques. These techniques were based on gas chromatography with electron capture detection on two stationary phases with different selectivity for the separation of PCB congeners and gas chromatography with mass spectrometric detection. The results from these three techniques were in good agreement and were combined to provide certified concentrations for 27 PCB congeners and 15 chlorinated pesticides.
Kaishun Bi - One of the best experts on this subject based on the ideXlab platform.
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uflc ms ms method for simultaneous determination of six lignans of schisandra chinensis turcz baill in normal and insomniac rats brain microdialysates and Homogenate samples towards an in depth study for its sedative hypnotic activity
Journal of Mass Spectrometry, 2013Co-Authors: Qing Li, Dan Su, Xiuli Ou, Kelin Chen, Xiaohui Chen, Kaishun BiAbstract:Schisandra chinensis (Turcz.) Baill., a traditional Chinese medicine, has been clinically used for the treatment of insomnia for centuries. The insomnia mechanism and the possible active ingredients of S. chinensis remain largely unknown. The objective of this study was to develop a method to detect its components which could pass through the blood brain barrier (BBB) by determining the brain microdialysate and brain Tissue Homogenate samples and then obtain the pharmacokinetic profile in brain for comprehensive understanding of its hypnotic clinical efficacy. Therefore, an efficient, sensitive and selective ultra fast liquid chromatography/tandem mass spectrometry method for the simultaneous determination of six sedative and hypnotic lignans (schisandrin, schisandrol B, schisantherin A, deoxyshisandrin, γ-schisandrin and gomisin N) of Schisandra chinensis (Turcz.) Baill. in rat brain Tissue Homogenate and brain microdialysates has been developed and validated. The analysis was performed on a Shim-pack XR-ODS column (75 mm × 3.0 mm, 2.2 µm) using gradient elution with the mobile phase consisting of acetonitrile and 0.1% formic acid water. The method was validated in brain Homogenate and microdialysate samples, which all showed good linearity over a wide concentration range (r2 > 0.99), and the obtained lower limit of quantification was 0.1 ng · ml−1 for the analytes in brain microdialysate samples. The intra- and inter-day assay variability was less than 15% for all analytes. The study proved the six lignans, as sedative and hypnotic ingredients, could pass through the BBB with brain targeting, distributed mainly in the hypothalamus and possessed complete pharmacokinetics process in brain. The results also indicated that significant difference in pharmacokinetic parameters of the analytes was observed between two groups, while absorptions of these analytes in insomniac group were significantly better than those in normal group. Copyright © 2013 John Wiley & Sons, Ltd.
