The Experts below are selected from a list of 99 Experts worldwide ranked by ideXlab platform
Victor J. Marks - One of the best experts on this subject based on the ideXlab platform.
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Rapid Toluidine Blue Stain for Mohs' Micrographic Surgery
Dermatologic surgery : official publication for American Society for Dermatologic Surgery [et al.], 2006Co-Authors: Michael M. Todd, James W. Lee, Victor J. MarksAbstract:Background Toluidine Blue is a useful Stain for detecting basal cell carcinoma during Mohs' micrographic surgery. Objective To demonstrate the efficacy of alkalinization on the Toluidine Blue Stain. Methods A 1% aqueous Toluidine Blue–1% aqueous sodium borate solution was used to Stain microscope slides for basal cell carcinoma during Mohs' micrographic surgery. Results Total Toluidine Blue Staining time was reduced to less than 2.5 minutes, without compromising the quality of the Stain. Conclusions The rapid Toluidine Blue Stain reduces Staining time while maintaining Staining quality, including the advantages specific to the Toluidine Blue Stain. MICHAEL M. TODD, MD, JAMES W. LEE, HT(ASCP), AND VICTOR J. MARKS, MD, HAVE INDICATED NO SIGNIFICANT INTEREST WITH COMMERCIAL SUPPORTERS.
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Rapid Toluidine Blue Stain for Mohs' Micrographic Surgery
Dermatologic surgery : official publication for American Society for Dermatologic Surgery [et al.], 2006Co-Authors: Michael M. Todd, James W. Lee, Victor J. MarksAbstract:Toluidine Blue is a useful Stain for detecting basal cell carcinoma during Mohs' micrographic surgery. To demonstrate the efficacy of alkalinization on the Toluidine Blue Stain. A 1% aqueous Toluidine Blue-1% aqueous sodium borate solution was used to Stain microscope slides for basal cell carcinoma during Mohs' micrographic surgery. Total Toluidine Blue Staining time was reduced to less than 2.5 minutes, without compromising the quality of the Stain. The rapid Toluidine Blue Stain reduces Staining time while maintaining Staining quality, including the advantages specific to the Toluidine Blue Stain.
Hongyang Wang - One of the best experts on this subject based on the ideXlab platform.
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Analysis of microdissected cells by two-dimensional LC-MS approaches.
Methods in molecular biology (Clifton N.J.), 2008Co-Authors: Yi-hong, Ye Xiong Tan, Hu Zhou, Lei Zhang, Qi Chang Xia, Hongyang WangAbstract:Laser capture microdissection (LCM) is a powerful tool that enables the isolation of specific cell types from tissue sections, overcoming the problem of tissue heterogeneity and contamination. We combined the LCM with isotope-coded affinity tag (ICAT) technology and two-dimensional liquid chromatography to investigate the qualitative and quantitative proteomes of hepatocellular carcinoma (HCC). The effects of three different histochemical Stains on tissue sections have been compared, and Toluidine Blue Stain was proved as the most suitable Stain for LCM followed by proteomic analysis. The solubilized proteins from microdissected HCC and non-HCC hepatocytes were qualitatively and quantitatively analyzed with two-dimensional liquid chromatography tandem mass spectrometry (2D-LC-MS/MS) alone or coupled with cleavable isotope-coded affinity tag (cICAT) labeling technology. A total of 644 proteins were qualitatively identified and 261 proteins were unambiguously quantified. These results showed that the clinical proteomic method using LCM coupled with ICAT and 2D-LC-MS/MS can carry out not only large-scale but also accurate qualitative and quantitative analysis.
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Accurate Qualitative and Quantitative Proteomic Analysis of Clinical Hepatocellular Carcinoma Using Laser Capture Microdissection Coupled with Isotope-coded Affinity Tag and Two-dimensional Liquid Chromatography Mass Spectrometry
Molecular & cellular proteomics : MCP, 2004Co-Authors: Yi Hong, Ye Xiong Tan, Hu Zhou, Lei Zhang, Qi Chang Xia, Hongyang WangAbstract:Laser capture microdissection (LCM) is a powerful tool that enables the isolation of specific cell types from tissue sections, overcoming the problem of tissue heterogeneity and contamination. This study combined the LCM with isotope-coded affinity tag (ICAT) technology and two-dimensional liquid chromatography to investigate the qualitative and quantitative proteomes of hepatocellular carcinoma (HCC). The effects of three different histochemical Stains on tissue sections have been compared, and Toluidine Blue Stain was proved as the most suitable Stain for LCM followed by proteomic analysis. The solubilized proteins from microdissected HCC and non-HCC hepatocytes were qualitatively and quantitatively analyzed with two-dimensional liquid chromatography tandem mass spectrometry (2D-LC-MS/MS) alone or coupled with cleavable ICAT labeling technology. A total of 644 proteins were qualitative identified, and 261 proteins were unambiguously quantitated. These results show that the clinical proteomic method using LCM coupled with ICAT and 2D-LC-MS/MS can carry out not only large-scale but also accurate qualitative and quantitative analysis.
Joel B Epstein - One of the best experts on this subject based on the ideXlab platform.
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analysis of oral lesion biopsies identified and evaluated by visual examination chemiluminescence and Toluidine Blue
Oral Oncology, 2008Co-Authors: Joel B Epstein, Joshua D Epstein, S A Lonky, Sol Silverman, M A BrideAbstract:Summary Conventional visual examination and palpation remains the gold-standard for the identification of oral mucosal lesions. The purpose of this study was to investigate the adjunctive value of a chemiluminescent light source (ViziLite ® , Zila Pharmaceuticals, Phoenix, Arizona) and application of pharmaceutical grade Toluidine Blue (TBlue 630™ , Zila Pharmaceuticals, Phoenix, Arizona) to further assess lesions identified during the conventional oral soft tissue examination. Lesions deemed clinically suspicious by visual examination under incandescent light were further assessed under chemiluminescence and then application of Toluidine Blue Stain. Differences between the conventional visual examination and chemiluminescent examination were noted on four characteristics which may aid in lesion identification. Tissue retention of Toluidine Blue Stain was documented. Each suspicious lesion was biopsied and diagnosed based upon routine histopathology. Both adjunctive exams were evaluated by comparing the histologic diagnosis. The additive value of Toluidine Blue Stain retention was assessed in lesions diagnosed as “serious pathology” defined as severe dysplasia, carcinoma in situ and squamous cell carcinoma. Ninety-seven clinically suspicious lesions in 84 patients were identified. The chemiluminescent exam improved the brightness and/or sharpness of margin in 61.8% of identified lesions. Biopsied lesions with Toluidine Blue Stain retention reduced the false positive rate by 55.26% while maintaining a 100% negative predictive value (NPV). Chemiluminescence was shown to increase the brightness and margins of mucosal lesions in a majority of cases and therefore may assist in identification of mucosal lesions not considered under traditional visual examination. Toluidine Blue Stain retention was associated with a large reduction in biopsies showing benign histology (false positive biopsy results), while maintaining a 100% NPV for the presence of severe dysplasia or cancer. Practitioners may consider use of these adjuncts in practice, however the results presented are based upon experienced providers in referral centers for mucosal disease or cancer centers and therefore positive findings may be an indication for referral to experienced providers.
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Toluidine Blue Staining Identifies High-Risk Primary Oral Premalignant Lesions with Poor Outcome
Cancer research, 2005Co-Authors: Lewei Zhang, Joel B Epstein, Michele Williams, Catherine F. Poh, Denise M. Laronde, Scott Durham, Hisae Nakamura, Ken Berean, Alan HovanAbstract:There is a pressing need for the development of visual aids that will facilitate the detection of oral premalignant lesions (OPLs) with a high-risk of progression. Preliminary data suggest that Toluidine Blue Stain may be preferentially retained by OPLs with high-risk molecular clones. In this study, we monitored OPLs from 100 patients without any history of oral cancer for an average of 44 months in order to evaluate the association of Toluidine Blue status with clinicopathologic risk factors, molecular patterns (microsatellite analysis on seven chromosome arms: 3p, 9p, 4q, 8p, 11q, 13q, and 17p) and outcome. Toluidine Blue-positive Staining correlated with clinicopathologic risk factors and high-risk molecular risk patterns. Significantly, a >6-fold elevation in cancer risk was observed for Toluidine Blue-positive lesions, with positive retention of the dye present in 12 of the 15 lesions that later progressed to cancer (P = 0.0008). This association of Toluidine Blue status with risk factors and outcome was evident even when the analysis was restricted to OPLs with low-grade or no dysplasia. Our results suggest the potential use of Toluidine Blue in identifying high-risk OPLs.
Alan Hovan - One of the best experts on this subject based on the ideXlab platform.
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Toluidine Blue Staining Identifies High-Risk Primary Oral Premalignant Lesions with Poor Outcome
Cancer research, 2005Co-Authors: Lewei Zhang, Joel B Epstein, Michele Williams, Catherine F. Poh, Denise M. Laronde, Scott Durham, Hisae Nakamura, Ken Berean, Alan HovanAbstract:There is a pressing need for the development of visual aids that will facilitate the detection of oral premalignant lesions (OPLs) with a high-risk of progression. Preliminary data suggest that Toluidine Blue Stain may be preferentially retained by OPLs with high-risk molecular clones. In this study, we monitored OPLs from 100 patients without any history of oral cancer for an average of 44 months in order to evaluate the association of Toluidine Blue status with clinicopathologic risk factors, molecular patterns (microsatellite analysis on seven chromosome arms: 3p, 9p, 4q, 8p, 11q, 13q, and 17p) and outcome. Toluidine Blue-positive Staining correlated with clinicopathologic risk factors and high-risk molecular risk patterns. Significantly, a >6-fold elevation in cancer risk was observed for Toluidine Blue-positive lesions, with positive retention of the dye present in 12 of the 15 lesions that later progressed to cancer (P = 0.0008). This association of Toluidine Blue status with risk factors and outcome was evident even when the analysis was restricted to OPLs with low-grade or no dysplasia. Our results suggest the potential use of Toluidine Blue in identifying high-risk OPLs.
Michael M. Todd - One of the best experts on this subject based on the ideXlab platform.
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Rapid Toluidine Blue Stain for Mohs' Micrographic Surgery
Dermatologic surgery : official publication for American Society for Dermatologic Surgery [et al.], 2006Co-Authors: Michael M. Todd, James W. Lee, Victor J. MarksAbstract:Background Toluidine Blue is a useful Stain for detecting basal cell carcinoma during Mohs' micrographic surgery. Objective To demonstrate the efficacy of alkalinization on the Toluidine Blue Stain. Methods A 1% aqueous Toluidine Blue–1% aqueous sodium borate solution was used to Stain microscope slides for basal cell carcinoma during Mohs' micrographic surgery. Results Total Toluidine Blue Staining time was reduced to less than 2.5 minutes, without compromising the quality of the Stain. Conclusions The rapid Toluidine Blue Stain reduces Staining time while maintaining Staining quality, including the advantages specific to the Toluidine Blue Stain. MICHAEL M. TODD, MD, JAMES W. LEE, HT(ASCP), AND VICTOR J. MARKS, MD, HAVE INDICATED NO SIGNIFICANT INTEREST WITH COMMERCIAL SUPPORTERS.
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Rapid Toluidine Blue Stain for Mohs' Micrographic Surgery
Dermatologic surgery : official publication for American Society for Dermatologic Surgery [et al.], 2006Co-Authors: Michael M. Todd, James W. Lee, Victor J. MarksAbstract:Toluidine Blue is a useful Stain for detecting basal cell carcinoma during Mohs' micrographic surgery. To demonstrate the efficacy of alkalinization on the Toluidine Blue Stain. A 1% aqueous Toluidine Blue-1% aqueous sodium borate solution was used to Stain microscope slides for basal cell carcinoma during Mohs' micrographic surgery. Total Toluidine Blue Staining time was reduced to less than 2.5 minutes, without compromising the quality of the Stain. The rapid Toluidine Blue Stain reduces Staining time while maintaining Staining quality, including the advantages specific to the Toluidine Blue Stain.