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Partha Roy - One of the best experts on this subject based on the ideXlab platform.

  • Determination of bioavailable-zinc from biofortified wheat using a coupled in vitro digestion/Caco-2 reporter-gene based Assay
    Journal of Food Composition and Analysis, 2012
    Co-Authors: Rajani Salunke, Nidhi Rawat, Vijay K. Tiwari, Kumari Neelam, Gursharn S. Randhawa, Harcharan Singh Dhaliwal, Partha Roy
    Abstract:

    Abstract The zinc bioavailability from wheat- Aegilops derivatives and Triticum monococcum low phytic acid ( lpa ) mutants with varying zinc concentrations was assessed using a novel metal response element (MRE)-binding transcription factor-1 (MTF-1)/MRE luciferase reporter gene based Transactivation Assay in the coupled in vitro digestion/Caco-2 model. The zinc content in the genotypes studied ranged from 19 to 65 μg/g. The bioavailability enhancement of zinc as determined from the luciferase response of MTF1/MRE transfected cells to cooked digests of biofortified derivatives and the lpa mutants was recorded as a 1.2–2.0 fold-increase, corresponding to 1.8–3.3 fold increases in the zinc content over the respective WL711-cultivar and T. monococcum wild-type controls. The strong positive and negative correlations observed for zinc bioavailability with respect to zinc content and phytate:zinc molar ratios in the derivatives ( r  = 0.74 and −0.76) as well as lpa mutants ( r  = 0.89 and −0.94) illustrate the significance of using these parameters in zinc biofortification programmes. Genotypes with phytate:zinc molar ratios of less than 15, with remarkably enhanced zinc bioavailability, were identified. This method could thus serve as a sensitive Transactivation Assay for a screening approach that could accurately rank bioavailability of zinc from biofortified staples for subsequent animal studies and advance select lines for human studies.

  • Detection of potential (anti)progestagenic endocrine disruptors using a recombinant human progesterone receptor binding and Transactivation Assay.
    Molecular and cellular endocrinology, 2008
    Co-Authors: Gunda Viswanath, Sujata Halder, Gunda Divya, Chandrajeet B Majumder, Partha Roy
    Abstract:

    The present work describes the identification of (anti)progestin endocrine disrupting chemicals (EDC) using a two step screening system. In the first step a competitive binding Assay was developed using recombinant human progesterone receptor (hPR). The tested chemicals were of various classes like insecticides, their metabolites, industrial chemicals and waste water treatment plant (WWTP) effluents. All the tested chemicals demonstrated a high affinity binding for hPR. The average IC50 values of the test chemicals were within the range of 1-25microM. In the second step of screening, a mammalian cell-based hPR Transactivation Assay was developed where HEK 293 cells were co-transfected with hPR and luciferase reporter gene under the control of progesterone-response element. Stimulation of the cells with progesterone resulted in about 25-fold up regulation of luciferase activity, with EC50 value of 4nM. Potent anti-progesterone, RU486, significantly inhibited progesterone-induced Transactivation and non-progestagenic steroids failed to transactivate hPR till 1microM concentrations. The chemicals showing high binding affinities in competitive binding Assays were then tested in Transactivation Assay and all of them were found to be anti-progestative except WWTP effluents. Transactivation Assays using extracted water samples from five different WWTP effluents showed that it was rich in progestative compounds. The levels of induction caused by these effluents were in the range of 15-25% of induction by progesterone and they represented about 6ng/l equivalent progesterone activities. In conclusion, we demonstrated that this two step Assay provides an efficient screening tool for the detection of (anti)progestative EDC in various samples.

Angelo Petrozza - One of the best experts on this subject based on the ideXlab platform.

  • a tilling allele of the tomato aux iaa9 gene offers new insights into fruit set mechanisms and perspectives for breeding seedless tomatoes
    Molecular Breeding, 2015
    Co-Authors: Andrea Mazzucato, Francesco Cellini, Mondher Bouzayen, Mohamed Zouine, Isabelle Mila, Silvia Minoia, Angelo Petrozza
    Abstract:

    Parthenocarpy is a desired trait in fruit crops; it enables fruit set under environmental conditions suboptimal for pollination, and seedless fruits represent a valuable consumer product. We employed TILLING-based screening of a mutant tomato population to find genetic lesions in Aux/IAA9, a negative regulator of the auxin response involved in the control of fruit set. We identified three mutations located in the coding region of this gene, including two singlebase substitutions and one single-base deletion, which leads to a frame shift and premature stop codon. The transcription of IAA9 was strongly reduced in the frame-shift mutant, and partial loss of mutated protein activity was evidenced by an in vitro Transactivation Assay. Whereas missense mutations were predicted to be tolerated and did not cause mutant phenotypes, the frame-shift mutation-induced phenotypes expected for a loss of IAA9 function, including altered axillary shoot growth, reduced leaf compoundness and a strong tendency to produce parthenocarpic fruits. Mutant flowers showed pleiotropic anther cone defects, a phenotype frequently associated with parthenocarpy in tomato and other species. Mutant fruits were larger than those of the seeded control, with higher brix

  • A TILLING allele of the tomato Aux/IAA9 gene offers new insights into fruit set mechanisms and perspectives for breeding seedless tomatoes
    Molecular Breeding, 2015
    Co-Authors: Andrea Mazzucato, Francesco Cellini, Mondher Bouzayen, Mohamed Zouine, Isabelle Mila, Silvia Minoia, Angelo Petrozza, Maurizio E. Picarella, Fabrizio Ruiu, Filomena Carriero
    Abstract:

    Parthenocarpy is a desired trait in fruit crops; it enables fruit set under environmental conditions suboptimal for pollination, and seedless fruits represent a valuable consumer product. We employed TILLING-based screening of a mutant tomato population to find genetic lesions in Aux/IAA9, a negative regulator of the auxin response involved in the control of fruit set. We identified three mutations located in the coding region of this gene, including two singlebase substitutions and one single-base deletion, which leads to a frame shift and premature stop codon. The transcription of IAA9 was strongly reduced in the frame-shift mutant, and partial loss of mutated protein activity was evidenced by an in vitro Transactivation Assay. Whereas missense mutations were predicted to be tolerated and did not cause mutant phenotypes, the frame-shift mutation-induced phenotypes expected for a loss of IAA9 function, including altered axillary shoot growth, reduced leaf compoundness and a strong tendency to produce parthenocarpic fruits. Mutant flowers showed pleiotropic anther cone defects, a phenotype frequently associated with parthenocarpy in tomato and other species. Mutant fruits were larger than those of the seeded control, with higher brix

Mondher Bouzayen - One of the best experts on this subject based on the ideXlab platform.

  • a tilling allele of the tomato aux iaa9 gene offers new insights into fruit set mechanisms and perspectives for breeding seedless tomatoes
    Molecular Breeding, 2015
    Co-Authors: Andrea Mazzucato, Francesco Cellini, Mondher Bouzayen, Mohamed Zouine, Isabelle Mila, Silvia Minoia, Angelo Petrozza
    Abstract:

    Parthenocarpy is a desired trait in fruit crops; it enables fruit set under environmental conditions suboptimal for pollination, and seedless fruits represent a valuable consumer product. We employed TILLING-based screening of a mutant tomato population to find genetic lesions in Aux/IAA9, a negative regulator of the auxin response involved in the control of fruit set. We identified three mutations located in the coding region of this gene, including two singlebase substitutions and one single-base deletion, which leads to a frame shift and premature stop codon. The transcription of IAA9 was strongly reduced in the frame-shift mutant, and partial loss of mutated protein activity was evidenced by an in vitro Transactivation Assay. Whereas missense mutations were predicted to be tolerated and did not cause mutant phenotypes, the frame-shift mutation-induced phenotypes expected for a loss of IAA9 function, including altered axillary shoot growth, reduced leaf compoundness and a strong tendency to produce parthenocarpic fruits. Mutant flowers showed pleiotropic anther cone defects, a phenotype frequently associated with parthenocarpy in tomato and other species. Mutant fruits were larger than those of the seeded control, with higher brix

  • A TILLING allele of the tomato Aux/IAA9 gene offers new insights into fruit set mechanisms and perspectives for breeding seedless tomatoes
    Molecular Breeding, 2015
    Co-Authors: Andrea Mazzucato, Francesco Cellini, Mondher Bouzayen, Mohamed Zouine, Isabelle Mila, Silvia Minoia, Angelo Petrozza, Maurizio E. Picarella, Fabrizio Ruiu, Filomena Carriero
    Abstract:

    Parthenocarpy is a desired trait in fruit crops; it enables fruit set under environmental conditions suboptimal for pollination, and seedless fruits represent a valuable consumer product. We employed TILLING-based screening of a mutant tomato population to find genetic lesions in Aux/IAA9, a negative regulator of the auxin response involved in the control of fruit set. We identified three mutations located in the coding region of this gene, including two singlebase substitutions and one single-base deletion, which leads to a frame shift and premature stop codon. The transcription of IAA9 was strongly reduced in the frame-shift mutant, and partial loss of mutated protein activity was evidenced by an in vitro Transactivation Assay. Whereas missense mutations were predicted to be tolerated and did not cause mutant phenotypes, the frame-shift mutation-induced phenotypes expected for a loss of IAA9 function, including altered axillary shoot growth, reduced leaf compoundness and a strong tendency to produce parthenocarpic fruits. Mutant flowers showed pleiotropic anther cone defects, a phenotype frequently associated with parthenocarpy in tomato and other species. Mutant fruits were larger than those of the seeded control, with higher brix

Mohamed Zouine - One of the best experts on this subject based on the ideXlab platform.

  • a tilling allele of the tomato aux iaa9 gene offers new insights into fruit set mechanisms and perspectives for breeding seedless tomatoes
    Molecular Breeding, 2015
    Co-Authors: Andrea Mazzucato, Francesco Cellini, Mondher Bouzayen, Mohamed Zouine, Isabelle Mila, Silvia Minoia, Angelo Petrozza
    Abstract:

    Parthenocarpy is a desired trait in fruit crops; it enables fruit set under environmental conditions suboptimal for pollination, and seedless fruits represent a valuable consumer product. We employed TILLING-based screening of a mutant tomato population to find genetic lesions in Aux/IAA9, a negative regulator of the auxin response involved in the control of fruit set. We identified three mutations located in the coding region of this gene, including two singlebase substitutions and one single-base deletion, which leads to a frame shift and premature stop codon. The transcription of IAA9 was strongly reduced in the frame-shift mutant, and partial loss of mutated protein activity was evidenced by an in vitro Transactivation Assay. Whereas missense mutations were predicted to be tolerated and did not cause mutant phenotypes, the frame-shift mutation-induced phenotypes expected for a loss of IAA9 function, including altered axillary shoot growth, reduced leaf compoundness and a strong tendency to produce parthenocarpic fruits. Mutant flowers showed pleiotropic anther cone defects, a phenotype frequently associated with parthenocarpy in tomato and other species. Mutant fruits were larger than those of the seeded control, with higher brix

  • A TILLING allele of the tomato Aux/IAA9 gene offers new insights into fruit set mechanisms and perspectives for breeding seedless tomatoes
    Molecular Breeding, 2015
    Co-Authors: Andrea Mazzucato, Francesco Cellini, Mondher Bouzayen, Mohamed Zouine, Isabelle Mila, Silvia Minoia, Angelo Petrozza, Maurizio E. Picarella, Fabrizio Ruiu, Filomena Carriero
    Abstract:

    Parthenocarpy is a desired trait in fruit crops; it enables fruit set under environmental conditions suboptimal for pollination, and seedless fruits represent a valuable consumer product. We employed TILLING-based screening of a mutant tomato population to find genetic lesions in Aux/IAA9, a negative regulator of the auxin response involved in the control of fruit set. We identified three mutations located in the coding region of this gene, including two singlebase substitutions and one single-base deletion, which leads to a frame shift and premature stop codon. The transcription of IAA9 was strongly reduced in the frame-shift mutant, and partial loss of mutated protein activity was evidenced by an in vitro Transactivation Assay. Whereas missense mutations were predicted to be tolerated and did not cause mutant phenotypes, the frame-shift mutation-induced phenotypes expected for a loss of IAA9 function, including altered axillary shoot growth, reduced leaf compoundness and a strong tendency to produce parthenocarpic fruits. Mutant flowers showed pleiotropic anther cone defects, a phenotype frequently associated with parthenocarpy in tomato and other species. Mutant fruits were larger than those of the seeded control, with higher brix

Isabelle Mila - One of the best experts on this subject based on the ideXlab platform.

  • a tilling allele of the tomato aux iaa9 gene offers new insights into fruit set mechanisms and perspectives for breeding seedless tomatoes
    Molecular Breeding, 2015
    Co-Authors: Andrea Mazzucato, Francesco Cellini, Mondher Bouzayen, Mohamed Zouine, Isabelle Mila, Silvia Minoia, Angelo Petrozza
    Abstract:

    Parthenocarpy is a desired trait in fruit crops; it enables fruit set under environmental conditions suboptimal for pollination, and seedless fruits represent a valuable consumer product. We employed TILLING-based screening of a mutant tomato population to find genetic lesions in Aux/IAA9, a negative regulator of the auxin response involved in the control of fruit set. We identified three mutations located in the coding region of this gene, including two singlebase substitutions and one single-base deletion, which leads to a frame shift and premature stop codon. The transcription of IAA9 was strongly reduced in the frame-shift mutant, and partial loss of mutated protein activity was evidenced by an in vitro Transactivation Assay. Whereas missense mutations were predicted to be tolerated and did not cause mutant phenotypes, the frame-shift mutation-induced phenotypes expected for a loss of IAA9 function, including altered axillary shoot growth, reduced leaf compoundness and a strong tendency to produce parthenocarpic fruits. Mutant flowers showed pleiotropic anther cone defects, a phenotype frequently associated with parthenocarpy in tomato and other species. Mutant fruits were larger than those of the seeded control, with higher brix

  • A TILLING allele of the tomato Aux/IAA9 gene offers new insights into fruit set mechanisms and perspectives for breeding seedless tomatoes
    Molecular Breeding, 2015
    Co-Authors: Andrea Mazzucato, Francesco Cellini, Mondher Bouzayen, Mohamed Zouine, Isabelle Mila, Silvia Minoia, Angelo Petrozza, Maurizio E. Picarella, Fabrizio Ruiu, Filomena Carriero
    Abstract:

    Parthenocarpy is a desired trait in fruit crops; it enables fruit set under environmental conditions suboptimal for pollination, and seedless fruits represent a valuable consumer product. We employed TILLING-based screening of a mutant tomato population to find genetic lesions in Aux/IAA9, a negative regulator of the auxin response involved in the control of fruit set. We identified three mutations located in the coding region of this gene, including two singlebase substitutions and one single-base deletion, which leads to a frame shift and premature stop codon. The transcription of IAA9 was strongly reduced in the frame-shift mutant, and partial loss of mutated protein activity was evidenced by an in vitro Transactivation Assay. Whereas missense mutations were predicted to be tolerated and did not cause mutant phenotypes, the frame-shift mutation-induced phenotypes expected for a loss of IAA9 function, including altered axillary shoot growth, reduced leaf compoundness and a strong tendency to produce parthenocarpic fruits. Mutant flowers showed pleiotropic anther cone defects, a phenotype frequently associated with parthenocarpy in tomato and other species. Mutant fruits were larger than those of the seeded control, with higher brix