The Experts below are selected from a list of 3168 Experts worldwide ranked by ideXlab platform
Ting Xu - One of the best experts on this subject based on the ideXlab platform.
-
Development of a one-step immunoassay for Triazophos using camel single-domain antibody–alkaline phosphatase fusion protein
Analytical and Bioanalytical Chemistry, 2019Co-Authors: Kai Wang, Zhiping Liu, Guochun Ding, Natalia Vasylieva, Shirley J. Gee, Dongyang Li, Bruce D. Hammock, Ji Li, Qing X Li, Ting XuAbstract:Triazophos is mainly used in Asian and African countries for the control of insects in agricultural production. Camelid variable domains of heavy-chain antibodies (VHHs) show great promise in monitoring environmental chemicals such as pesticides. To improve the rate of success in the generation of VHHs against Triazophos, genes specifically encoding VHH fragments from the unique allotype IgG3a of an immunized Camelus bactrianus were amplified by using a pair of novel primers and introduced to construct a diverse VHH library. Five out of seven isolated positive clones, including the VHH T1 with the highest affinity to Triazophos, were derived from the allotype IgG3a. A one-step enzyme-linked immunosorbent assay (ELISA) using VHH T1 genetically fused with alkaline phosphatase (AP) had a half-maximum inhibition concentration of 6.6 ng/mL for Triazophos. This assay showed negligible cross-reactivity with a list of important organophosphate pesticides (
-
development of a one step immunoassay for Triazophos using camel single domain antibody alkaline phosphatase fusion protein
Analytical and Bioanalytical Chemistry, 2019Co-Authors: Kai Wang, Guochun Ding, Natalia Vasylieva, Dongyang Li, Bruce D. Hammock, Ji Li, Qing X Li, Ting XuAbstract:Triazophos is mainly used in Asian and African countries for the control of insects in agricultural production. Camelid variable domains of heavy-chain antibodies (VHHs) show great promise in monitoring environmental chemicals such as pesticides. To improve the rate of success in the generation of VHHs against Triazophos, genes specifically encoding VHH fragments from the unique allotype IgG3a of an immunized Camelus bactrianus were amplified by using a pair of novel primers and introduced to construct a diverse VHH library. Five out of seven isolated positive clones, including the VHH T1 with the highest affinity to Triazophos, were derived from the allotype IgG3a. A one-step enzyme-linked immunosorbent assay (ELISA) using VHH T1 genetically fused with alkaline phosphatase (AP) had a half-maximum inhibition concentration of 6.6 ng/mL for Triazophos. This assay showed negligible cross-reactivity with a list of important organophosphate pesticides (< 0.1%). The average recoveries of Triazophos from water, soil, and apple samples determined by the one-step ELISA ranged from 83 to 108%, having a good correlation with those by a gas chromatography mass spectrometry (R2 = 0.99). The VHH-AP fusion protein shows potential for the analysis of Triazophos in various matrices.
Linquan Ge - One of the best experts on this subject based on the ideXlab platform.
-
the effects of Triazophos applied to transgenic bt rice on the nutritional indexes nlvg expression and population growth of nilaparvata lugens stal under elevated co2
Pesticide Biochemistry and Physiology, 2015Co-Authors: Linquan Ge, Jincai Wu, Fang Ouyang, Feng GeAbstract:Abstract The brown planthopper, Nilaparvata lugens (Stal) (Hemiptera: Delphacidae), is a typical pest in which population resurgence can be induced by insecticides. Warmer global temperatures, associated with anthropogenic climate change, are likely to have marked ecological effects on terrestrial ecosystems. However, the effects of elevated CO 2 (eCO 2 ) concentrations on the resurgence of N. lugens that have been treated with pesticides used for transgenic Bt rice cultivation are not fully understood. The present study investigated changes in the protein content, soluble sugar content, free amino acid level, vitellogenin ( Nlvg ) mRNA expression, and the population growth of N. lugens on transgenic Bt rice (TT51) following triazaophos foliar spray under conditions of eCO 2 . The results showed that the protein content in the fat bodies and ovaries of N. lugens adult females in TT51 treated with 40 ppm Triazophos under eCO 2 was significantly higher than under ambient CO 2 (aCO 2 ) and was also higher than that in females feeding on the non-transgenic parent (MH63) under aCO 2 at different days after emergence (DAEs). The soluble sugar content and free amino level of adult females in TT51 treated with 40 ppm Triazophos under eCO 2 was significantly higher than under aCO 2 and was also higher than in MH63 under aCO 2 at 1 and 3 DAE. The Nlvg mRNA expression level of N. lugens adult females in TT51 treated with 40 ppm Triazophos under eCO 2 was significantly higher than under aCO 2 and was also higher than in MH63 under aCO 2 at 1 and 3 DAE. The population number of N. lugens in TT51 treated with 40 ppm Triazophos under eCO 2 was significantly higher than under aCO 2 and was also higher than in MH63 under aCO 2 . The present findings provide important information for integrated pest management with transgenic varieties and a better understanding of the resurgence mechanism of N. lugens under eCO 2 .
-
effects of Triazophos on biochemical substances of transgenic bt rice and its nontarget pest nilaparvata lugens stal under elevated co2
Pesticide Biochemistry and Physiology, 2013Co-Authors: Linquan Ge, Jincai Wu, Fang Ouyang, Feng GeAbstract:The brown planthopper (BPH), Nilaparvata lugens Stal, is a serious rice pest throughout Asia. Recent outbreaks of N. lugens populations were mainly associated with the overuse of pesticides and resistance to insecticides. Warmer global temperatures that are associated with anthropogenic climate change are likely to have marked ecological effects on terrestrial ecosystems. However, the effects of elevated CO2 concentrations on the biochemical, physiological and nutrient quality of transgenic Bt rice that has been treated with pesticides and on the control efficacy of the pesticides are not understood. The present study investigated changes in soluble sugar content, free amino acid levels, oxalic acid levels, flavonoids levels, and Triazophos residues in transgenic Bt rice (TT51) and the control efficacy of Triazophos for N. lugens following Triazophos foliar spray under conditions of elevated CO2 (eCO2). Our findings showed that the soluble sugar content of TT51 treated with Triazophos under eCO2 was significantly higher than that under ambient CO2 (aCO2) and also higher than that of the non-transgenic parent (MH63) under aCO2. However, the results for free amino acid levels were the opposite of those for soluble sugar levels. The oxalic acid and flavonoid contents of rice plants significantly decreased with increases in Triazophos concentration, CO2 concentration, and days after treatment (DAT). The oxalic acid and flavonoid contents of TT51 treated with Triazophos under eCO2 were significantly lower than those under aCO2 and also lower than those of MH63 under aCO2. The residue concentration of Triazophos varied with CO2 concentration, rice variety, and DAT. The residues in TT51 treated with 80 ppm of trizaopos under eCO2 were significantly lower than those under aCO2 and those in MH63 under aCO2. The survival rate of nymphs N. lugens in TT51 under eCO2 was significantly higher than that under aCO2 and that in MH63 under aCO2 at 1 DAT or 15 DAT after the release of 2nd instars nymphs. These findings indicated that (1) for TT51, Triazophos reduced the resistance of rice plants to N. lugens with an elevated CO2 concentration, as N. lugens consumed more phloem sap on TT51 plants; (2) Triazophos dissipation in TT51 under eCO2 was significantly faster than that under aCO2 and that in MH63 under aCO2; (3) the control efficacy of Triazophos for N. lugens significantly decreased under eCO2. The present findings provide important information for integrated pest management among transgenic varieties.
-
the effects of Triazophos on the trehalose content trehalase activity and their gene expression in the brown planthopper nilaparvata lugens stal hemiptera delphacidae
Pesticide Biochemistry and Physiology, 2011Co-Authors: Linquan Ge, Kefei Zhao, Liujuan Huang, Jincai WuAbstract:A previous study demonstrated that the flight capacity of Nilaparvata lugens adults treated with Triazophos was enhanced significantly. However, the physiological and regulative mechanisms of the flight enhancement are not well understood. Trehalose is a primary blood sugar in insects, and the enzyme trehalase is involved in energy metabolism. The present study investigated the effects of Triazophos on the trehalose content, trehalase activity (soluble trehalase and membrane-bound trehalase) and the mRNA transcript levels of their corresponding genes (NlTre-1 and NlTre-2) in fifth instar nymphs, as well as in the brachypterous and macropterous N. lugens adult females. Our findings showed that the trehalose content in fifth instar nymphs as well as in the brachypterous and the macropterous adults significantly decreased following Triazophos treatment. However, the glucose content, soluble trehalase activity and expression level of NlTre-1 mRNA increased significantly compared to the controls. No significant enhancement of NlTre-2 expression was found, indicating that regulation of energy metabolism of Triazophos-induced flight capacity in N. lugens was not associated with NlTre-2 expression. In addition, soluble trehalase activity and the expression level of NlTre-1 mRNA in the macropterous females was significantly higher than that in the brachypterous females. The present findings provide valuable information on the molecular and regulative mechanisms of the increased flight capacity found in adult N. lugens after treatment with Triazophos.
Kai Wang - One of the best experts on this subject based on the ideXlab platform.
-
Development of a one-step immunoassay for Triazophos using camel single-domain antibody–alkaline phosphatase fusion protein
Analytical and Bioanalytical Chemistry, 2019Co-Authors: Kai Wang, Zhiping Liu, Guochun Ding, Natalia Vasylieva, Shirley J. Gee, Dongyang Li, Bruce D. Hammock, Ji Li, Qing X Li, Ting XuAbstract:Triazophos is mainly used in Asian and African countries for the control of insects in agricultural production. Camelid variable domains of heavy-chain antibodies (VHHs) show great promise in monitoring environmental chemicals such as pesticides. To improve the rate of success in the generation of VHHs against Triazophos, genes specifically encoding VHH fragments from the unique allotype IgG3a of an immunized Camelus bactrianus were amplified by using a pair of novel primers and introduced to construct a diverse VHH library. Five out of seven isolated positive clones, including the VHH T1 with the highest affinity to Triazophos, were derived from the allotype IgG3a. A one-step enzyme-linked immunosorbent assay (ELISA) using VHH T1 genetically fused with alkaline phosphatase (AP) had a half-maximum inhibition concentration of 6.6 ng/mL for Triazophos. This assay showed negligible cross-reactivity with a list of important organophosphate pesticides (
-
development of a one step immunoassay for Triazophos using camel single domain antibody alkaline phosphatase fusion protein
Analytical and Bioanalytical Chemistry, 2019Co-Authors: Kai Wang, Guochun Ding, Natalia Vasylieva, Dongyang Li, Bruce D. Hammock, Ji Li, Qing X Li, Ting XuAbstract:Triazophos is mainly used in Asian and African countries for the control of insects in agricultural production. Camelid variable domains of heavy-chain antibodies (VHHs) show great promise in monitoring environmental chemicals such as pesticides. To improve the rate of success in the generation of VHHs against Triazophos, genes specifically encoding VHH fragments from the unique allotype IgG3a of an immunized Camelus bactrianus were amplified by using a pair of novel primers and introduced to construct a diverse VHH library. Five out of seven isolated positive clones, including the VHH T1 with the highest affinity to Triazophos, were derived from the allotype IgG3a. A one-step enzyme-linked immunosorbent assay (ELISA) using VHH T1 genetically fused with alkaline phosphatase (AP) had a half-maximum inhibition concentration of 6.6 ng/mL for Triazophos. This assay showed negligible cross-reactivity with a list of important organophosphate pesticides (< 0.1%). The average recoveries of Triazophos from water, soil, and apple samples determined by the one-step ELISA ranged from 83 to 108%, having a good correlation with those by a gas chromatography mass spectrometry (R2 = 0.99). The VHH-AP fusion protein shows potential for the analysis of Triazophos in various matrices.
Feng Ge - One of the best experts on this subject based on the ideXlab platform.
-
the effects of Triazophos applied to transgenic bt rice on the nutritional indexes nlvg expression and population growth of nilaparvata lugens stal under elevated co2
Pesticide Biochemistry and Physiology, 2015Co-Authors: Linquan Ge, Jincai Wu, Fang Ouyang, Feng GeAbstract:Abstract The brown planthopper, Nilaparvata lugens (Stal) (Hemiptera: Delphacidae), is a typical pest in which population resurgence can be induced by insecticides. Warmer global temperatures, associated with anthropogenic climate change, are likely to have marked ecological effects on terrestrial ecosystems. However, the effects of elevated CO 2 (eCO 2 ) concentrations on the resurgence of N. lugens that have been treated with pesticides used for transgenic Bt rice cultivation are not fully understood. The present study investigated changes in the protein content, soluble sugar content, free amino acid level, vitellogenin ( Nlvg ) mRNA expression, and the population growth of N. lugens on transgenic Bt rice (TT51) following triazaophos foliar spray under conditions of eCO 2 . The results showed that the protein content in the fat bodies and ovaries of N. lugens adult females in TT51 treated with 40 ppm Triazophos under eCO 2 was significantly higher than under ambient CO 2 (aCO 2 ) and was also higher than that in females feeding on the non-transgenic parent (MH63) under aCO 2 at different days after emergence (DAEs). The soluble sugar content and free amino level of adult females in TT51 treated with 40 ppm Triazophos under eCO 2 was significantly higher than under aCO 2 and was also higher than in MH63 under aCO 2 at 1 and 3 DAE. The Nlvg mRNA expression level of N. lugens adult females in TT51 treated with 40 ppm Triazophos under eCO 2 was significantly higher than under aCO 2 and was also higher than in MH63 under aCO 2 at 1 and 3 DAE. The population number of N. lugens in TT51 treated with 40 ppm Triazophos under eCO 2 was significantly higher than under aCO 2 and was also higher than in MH63 under aCO 2 . The present findings provide important information for integrated pest management with transgenic varieties and a better understanding of the resurgence mechanism of N. lugens under eCO 2 .
-
effects of Triazophos on biochemical substances of transgenic bt rice and its nontarget pest nilaparvata lugens stal under elevated co2
Pesticide Biochemistry and Physiology, 2013Co-Authors: Linquan Ge, Jincai Wu, Fang Ouyang, Feng GeAbstract:The brown planthopper (BPH), Nilaparvata lugens Stal, is a serious rice pest throughout Asia. Recent outbreaks of N. lugens populations were mainly associated with the overuse of pesticides and resistance to insecticides. Warmer global temperatures that are associated with anthropogenic climate change are likely to have marked ecological effects on terrestrial ecosystems. However, the effects of elevated CO2 concentrations on the biochemical, physiological and nutrient quality of transgenic Bt rice that has been treated with pesticides and on the control efficacy of the pesticides are not understood. The present study investigated changes in soluble sugar content, free amino acid levels, oxalic acid levels, flavonoids levels, and Triazophos residues in transgenic Bt rice (TT51) and the control efficacy of Triazophos for N. lugens following Triazophos foliar spray under conditions of elevated CO2 (eCO2). Our findings showed that the soluble sugar content of TT51 treated with Triazophos under eCO2 was significantly higher than that under ambient CO2 (aCO2) and also higher than that of the non-transgenic parent (MH63) under aCO2. However, the results for free amino acid levels were the opposite of those for soluble sugar levels. The oxalic acid and flavonoid contents of rice plants significantly decreased with increases in Triazophos concentration, CO2 concentration, and days after treatment (DAT). The oxalic acid and flavonoid contents of TT51 treated with Triazophos under eCO2 were significantly lower than those under aCO2 and also lower than those of MH63 under aCO2. The residue concentration of Triazophos varied with CO2 concentration, rice variety, and DAT. The residues in TT51 treated with 80 ppm of trizaopos under eCO2 were significantly lower than those under aCO2 and those in MH63 under aCO2. The survival rate of nymphs N. lugens in TT51 under eCO2 was significantly higher than that under aCO2 and that in MH63 under aCO2 at 1 DAT or 15 DAT after the release of 2nd instars nymphs. These findings indicated that (1) for TT51, Triazophos reduced the resistance of rice plants to N. lugens with an elevated CO2 concentration, as N. lugens consumed more phloem sap on TT51 plants; (2) Triazophos dissipation in TT51 under eCO2 was significantly faster than that under aCO2 and that in MH63 under aCO2; (3) the control efficacy of Triazophos for N. lugens significantly decreased under eCO2. The present findings provide important information for integrated pest management among transgenic varieties.
Jincai Wu - One of the best experts on this subject based on the ideXlab platform.
-
the effects of Triazophos applied to transgenic bt rice on the nutritional indexes nlvg expression and population growth of nilaparvata lugens stal under elevated co2
Pesticide Biochemistry and Physiology, 2015Co-Authors: Linquan Ge, Jincai Wu, Fang Ouyang, Feng GeAbstract:Abstract The brown planthopper, Nilaparvata lugens (Stal) (Hemiptera: Delphacidae), is a typical pest in which population resurgence can be induced by insecticides. Warmer global temperatures, associated with anthropogenic climate change, are likely to have marked ecological effects on terrestrial ecosystems. However, the effects of elevated CO 2 (eCO 2 ) concentrations on the resurgence of N. lugens that have been treated with pesticides used for transgenic Bt rice cultivation are not fully understood. The present study investigated changes in the protein content, soluble sugar content, free amino acid level, vitellogenin ( Nlvg ) mRNA expression, and the population growth of N. lugens on transgenic Bt rice (TT51) following triazaophos foliar spray under conditions of eCO 2 . The results showed that the protein content in the fat bodies and ovaries of N. lugens adult females in TT51 treated with 40 ppm Triazophos under eCO 2 was significantly higher than under ambient CO 2 (aCO 2 ) and was also higher than that in females feeding on the non-transgenic parent (MH63) under aCO 2 at different days after emergence (DAEs). The soluble sugar content and free amino level of adult females in TT51 treated with 40 ppm Triazophos under eCO 2 was significantly higher than under aCO 2 and was also higher than in MH63 under aCO 2 at 1 and 3 DAE. The Nlvg mRNA expression level of N. lugens adult females in TT51 treated with 40 ppm Triazophos under eCO 2 was significantly higher than under aCO 2 and was also higher than in MH63 under aCO 2 at 1 and 3 DAE. The population number of N. lugens in TT51 treated with 40 ppm Triazophos under eCO 2 was significantly higher than under aCO 2 and was also higher than in MH63 under aCO 2 . The present findings provide important information for integrated pest management with transgenic varieties and a better understanding of the resurgence mechanism of N. lugens under eCO 2 .
-
effects of Triazophos on biochemical substances of transgenic bt rice and its nontarget pest nilaparvata lugens stal under elevated co2
Pesticide Biochemistry and Physiology, 2013Co-Authors: Linquan Ge, Jincai Wu, Fang Ouyang, Feng GeAbstract:The brown planthopper (BPH), Nilaparvata lugens Stal, is a serious rice pest throughout Asia. Recent outbreaks of N. lugens populations were mainly associated with the overuse of pesticides and resistance to insecticides. Warmer global temperatures that are associated with anthropogenic climate change are likely to have marked ecological effects on terrestrial ecosystems. However, the effects of elevated CO2 concentrations on the biochemical, physiological and nutrient quality of transgenic Bt rice that has been treated with pesticides and on the control efficacy of the pesticides are not understood. The present study investigated changes in soluble sugar content, free amino acid levels, oxalic acid levels, flavonoids levels, and Triazophos residues in transgenic Bt rice (TT51) and the control efficacy of Triazophos for N. lugens following Triazophos foliar spray under conditions of elevated CO2 (eCO2). Our findings showed that the soluble sugar content of TT51 treated with Triazophos under eCO2 was significantly higher than that under ambient CO2 (aCO2) and also higher than that of the non-transgenic parent (MH63) under aCO2. However, the results for free amino acid levels were the opposite of those for soluble sugar levels. The oxalic acid and flavonoid contents of rice plants significantly decreased with increases in Triazophos concentration, CO2 concentration, and days after treatment (DAT). The oxalic acid and flavonoid contents of TT51 treated with Triazophos under eCO2 were significantly lower than those under aCO2 and also lower than those of MH63 under aCO2. The residue concentration of Triazophos varied with CO2 concentration, rice variety, and DAT. The residues in TT51 treated with 80 ppm of trizaopos under eCO2 were significantly lower than those under aCO2 and those in MH63 under aCO2. The survival rate of nymphs N. lugens in TT51 under eCO2 was significantly higher than that under aCO2 and that in MH63 under aCO2 at 1 DAT or 15 DAT after the release of 2nd instars nymphs. These findings indicated that (1) for TT51, Triazophos reduced the resistance of rice plants to N. lugens with an elevated CO2 concentration, as N. lugens consumed more phloem sap on TT51 plants; (2) Triazophos dissipation in TT51 under eCO2 was significantly faster than that under aCO2 and that in MH63 under aCO2; (3) the control efficacy of Triazophos for N. lugens significantly decreased under eCO2. The present findings provide important information for integrated pest management among transgenic varieties.
-
the effects of Triazophos on the trehalose content trehalase activity and their gene expression in the brown planthopper nilaparvata lugens stal hemiptera delphacidae
Pesticide Biochemistry and Physiology, 2011Co-Authors: Linquan Ge, Kefei Zhao, Liujuan Huang, Jincai WuAbstract:A previous study demonstrated that the flight capacity of Nilaparvata lugens adults treated with Triazophos was enhanced significantly. However, the physiological and regulative mechanisms of the flight enhancement are not well understood. Trehalose is a primary blood sugar in insects, and the enzyme trehalase is involved in energy metabolism. The present study investigated the effects of Triazophos on the trehalose content, trehalase activity (soluble trehalase and membrane-bound trehalase) and the mRNA transcript levels of their corresponding genes (NlTre-1 and NlTre-2) in fifth instar nymphs, as well as in the brachypterous and macropterous N. lugens adult females. Our findings showed that the trehalose content in fifth instar nymphs as well as in the brachypterous and the macropterous adults significantly decreased following Triazophos treatment. However, the glucose content, soluble trehalase activity and expression level of NlTre-1 mRNA increased significantly compared to the controls. No significant enhancement of NlTre-2 expression was found, indicating that regulation of energy metabolism of Triazophos-induced flight capacity in N. lugens was not associated with NlTre-2 expression. In addition, soluble trehalase activity and the expression level of NlTre-1 mRNA in the macropterous females was significantly higher than that in the brachypterous females. The present findings provide valuable information on the molecular and regulative mechanisms of the increased flight capacity found in adult N. lugens after treatment with Triazophos.
