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Ran Duan - One of the best experts on this subject based on the ideXlab platform.
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arsenite resistance accumulation and volatilization properties of Trichoderma asperellum sm 12f1 penicillium janthinellum sm 12f4 and fusarium oxysporum cz 8f1
Clean-soil Air Water, 2015Co-Authors: Qiufen Feng, Xibai Zeng, Yangzhu Zhang, Lingyu Bai, Ran Duan, Zhiling LinAbstract:It is expected that microorganisms capable of arsenic (As) accumulation and volatilization can be used to remediate As-contaminated environments. In this paper, the arsenite [As(III)] resistance, accumulation, and volatilization properties of Trichoderma asperellum SM-12F1, Penicillium janthinellum SM-12F4, and Fusarium oxysporum CZ-8F1 were investigated. The results indicated that all three fungal strains exhibited significant As(III)-resistance. After being exposed to 40 and 80 mg/L As(III) for five days, the dry mycelium weights of P. janthinellum SM-12F4 and F. oxysporum CZ-8F1 were significantly increased compared to the controls. The spiked As(III) initially inhibited and subsequently promoted fungal growth (mainly in the logarithmic growth phase). The maximal biomasses of 0.25, 0.29, and 0.29 g for T. asperellum SM-12F1, P. janthinellum SM-12F4, and F. oxysporum CZ-8F1 after exposure to As(III), respectively, were all higher than their corresponding controls without As(III). When exposed to 40 mg/L As(III) for five days, the efficiencies of As(III) accumulation for T. asperellum SM-12F1, P. janthinellum SM-12F4, and F. oxysporum CZ-8F1 were 53.02, 13.67, and 13.65 µg/g/day, respectively, and the efficiencies of As(III) volatilization were 51.87, 54.34, and 46.35 µg/g/day, respectively. It seems that these fungal strains can be used in the future to tackle As-contaminated environments.
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arsenate reduction and methylation in the cells of Trichoderma asperellum sm 12f1 penicillium janthinellum sm 12f4 and fusarium oxysporum cz 8f1 investigated with x ray absorption near edge structure
Journal of Hazardous Materials, 2012Co-Authors: Xibai Zeng, Ran Duan, L Y Bai, Jinfen Wang, Sheng JiangAbstract:Synchrotron radiation-based X-ray absorption near edge structure (XANES) was introduced to directly analysis chemical species of arsenic (As) in the cells of Trichoderma asperellum SM-12F1, Penicillium janthinellum SM-12F4, and Fusarium oxysporum CZ-8F1 capable of As accumulation and volatilisation. After exposure to As(V) of 500 mg L-1 for 15 days, a total of 60.5% and 65.3% of the accumulated As in the cells of T. asperellum SM-12F1 and P. janthinellum SM-12F4, respectively, was As(III), followed by 31.3% and 32.4% DMA (dimethylarsinic acid), 8.3% and 2.3% MMA (monomethylarsonic acid), respectively. However, for F. oxysporum CZ-8F1, 54.5% of the accumulated As was As(III), followed by 37.8% MMA and 7.7% As(V). The reduction and methylation of As(V) formed As(III), MMA, and DMA as the primacy products, and the reduction of As(V) might be more easily processed than the methylation. These results will help to understanding the mechanisms of As detoxification and its future application in bioremediation. (C) 2012 Elsevier B.V. All rights reserved.
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arsenic biotransformation by arsenic resistant fungi Trichoderma asperellum sm 12f1 penicillium janthinellum sm 12f4 and fusarium oxysporum cz 8f1
Science of The Total Environment, 2011Co-Authors: Xibai Zeng, Lingyu Bai, Ran DuanAbstract:Abstract Bioremediation of arsenic (As)-contaminated soil using microorganisms has been a focus of research because it is environment friendly and cost-effective. The As-resistant fungi Trichoderma asperellum SM-12F1, Penicillium janthinellum SM-12F4, and Fusarium oxysporum CZ-8F1 were exposed to 50 mg l− 1 of As(V), and the biotransformation of As and the concomitant variance of Eh and pH in the media were studied. Fresh weights of all three isolates increased and then decreased depended on cultivation period. After cultivation for 2 or 3 days, the As(V) added to the media had been completely changed into As(III), whilst As(V) was predominate in fungal cells with concomitantly little As(III) during cultivation. After 15 days, little monomethylarsonic acid (MMA) and dimethylarsinic acid (DMA) besides of As(V) and As(III) were found in the cells of T. asperellum SM-12F1, and the total As content was the highest in cells of P. janthinellum SM-12F4 (about 41.5 μg) according to the quantitative analysis of As speciation in cultures. Moreover, when cultivation period reached 3 days, the Eh and pH in the media of T. asperellum SM-12F1 (312.5 mV and 4.8), P. janthinellum SM-12F4 (411.1 mV and 4.2), and F. oxysporum CZ-8F1 (269.4 mV and 4.8) might not responsible for the reduction of As(V) based on the previous study. Therefore, it is speculated that import/export, reduction, and methylation of As are conducted in fungal cells. Future studies investigating the biochemical behaviour of fungi responding to As are needed to gain a better understanding of bioremediation of As-contaminated soils.
Xibai Zeng - One of the best experts on this subject based on the ideXlab platform.
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arsenite resistance accumulation and volatilization properties of Trichoderma asperellum sm 12f1 penicillium janthinellum sm 12f4 and fusarium oxysporum cz 8f1
Clean-soil Air Water, 2015Co-Authors: Qiufen Feng, Xibai Zeng, Yangzhu Zhang, Lingyu Bai, Ran Duan, Zhiling LinAbstract:It is expected that microorganisms capable of arsenic (As) accumulation and volatilization can be used to remediate As-contaminated environments. In this paper, the arsenite [As(III)] resistance, accumulation, and volatilization properties of Trichoderma asperellum SM-12F1, Penicillium janthinellum SM-12F4, and Fusarium oxysporum CZ-8F1 were investigated. The results indicated that all three fungal strains exhibited significant As(III)-resistance. After being exposed to 40 and 80 mg/L As(III) for five days, the dry mycelium weights of P. janthinellum SM-12F4 and F. oxysporum CZ-8F1 were significantly increased compared to the controls. The spiked As(III) initially inhibited and subsequently promoted fungal growth (mainly in the logarithmic growth phase). The maximal biomasses of 0.25, 0.29, and 0.29 g for T. asperellum SM-12F1, P. janthinellum SM-12F4, and F. oxysporum CZ-8F1 after exposure to As(III), respectively, were all higher than their corresponding controls without As(III). When exposed to 40 mg/L As(III) for five days, the efficiencies of As(III) accumulation for T. asperellum SM-12F1, P. janthinellum SM-12F4, and F. oxysporum CZ-8F1 were 53.02, 13.67, and 13.65 µg/g/day, respectively, and the efficiencies of As(III) volatilization were 51.87, 54.34, and 46.35 µg/g/day, respectively. It seems that these fungal strains can be used in the future to tackle As-contaminated environments.
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arsenate reduction and methylation in the cells of Trichoderma asperellum sm 12f1 penicillium janthinellum sm 12f4 and fusarium oxysporum cz 8f1 investigated with x ray absorption near edge structure
Journal of Hazardous Materials, 2012Co-Authors: Xibai Zeng, Ran Duan, L Y Bai, Jinfen Wang, Sheng JiangAbstract:Synchrotron radiation-based X-ray absorption near edge structure (XANES) was introduced to directly analysis chemical species of arsenic (As) in the cells of Trichoderma asperellum SM-12F1, Penicillium janthinellum SM-12F4, and Fusarium oxysporum CZ-8F1 capable of As accumulation and volatilisation. After exposure to As(V) of 500 mg L-1 for 15 days, a total of 60.5% and 65.3% of the accumulated As in the cells of T. asperellum SM-12F1 and P. janthinellum SM-12F4, respectively, was As(III), followed by 31.3% and 32.4% DMA (dimethylarsinic acid), 8.3% and 2.3% MMA (monomethylarsonic acid), respectively. However, for F. oxysporum CZ-8F1, 54.5% of the accumulated As was As(III), followed by 37.8% MMA and 7.7% As(V). The reduction and methylation of As(V) formed As(III), MMA, and DMA as the primacy products, and the reduction of As(V) might be more easily processed than the methylation. These results will help to understanding the mechanisms of As detoxification and its future application in bioremediation. (C) 2012 Elsevier B.V. All rights reserved.
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arsenic biotransformation by arsenic resistant fungi Trichoderma asperellum sm 12f1 penicillium janthinellum sm 12f4 and fusarium oxysporum cz 8f1
Science of The Total Environment, 2011Co-Authors: Xibai Zeng, Lingyu Bai, Ran DuanAbstract:Abstract Bioremediation of arsenic (As)-contaminated soil using microorganisms has been a focus of research because it is environment friendly and cost-effective. The As-resistant fungi Trichoderma asperellum SM-12F1, Penicillium janthinellum SM-12F4, and Fusarium oxysporum CZ-8F1 were exposed to 50 mg l− 1 of As(V), and the biotransformation of As and the concomitant variance of Eh and pH in the media were studied. Fresh weights of all three isolates increased and then decreased depended on cultivation period. After cultivation for 2 or 3 days, the As(V) added to the media had been completely changed into As(III), whilst As(V) was predominate in fungal cells with concomitantly little As(III) during cultivation. After 15 days, little monomethylarsonic acid (MMA) and dimethylarsinic acid (DMA) besides of As(V) and As(III) were found in the cells of T. asperellum SM-12F1, and the total As content was the highest in cells of P. janthinellum SM-12F4 (about 41.5 μg) according to the quantitative analysis of As speciation in cultures. Moreover, when cultivation period reached 3 days, the Eh and pH in the media of T. asperellum SM-12F1 (312.5 mV and 4.8), P. janthinellum SM-12F4 (411.1 mV and 4.2), and F. oxysporum CZ-8F1 (269.4 mV and 4.8) might not responsible for the reduction of As(V) based on the previous study. Therefore, it is speculated that import/export, reduction, and methylation of As are conducted in fungal cells. Future studies investigating the biochemical behaviour of fungi responding to As are needed to gain a better understanding of bioremediation of As-contaminated soils.
Martínez Coca Benedicto - One of the best experts on this subject based on the ideXlab platform.
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Antagonismo de seis cepas de Trichoderma asperellum Samuels, Lieckfeldt & Nirenberg sobre Colletotrichum spp.
Centro Nacional de Sanidad Agropecuaria, 2021Co-Authors: Infante Martínez Danay, Martínez Coca BenedictoAbstract:The present work was aimed at evaluating the antagonistic effect of six strains of Trichoderma asperellum Samuels, Lieckfeldt & Nirenberg against two isolates of Colletotrichum spp., insolated from guava (Psidium guajava L.) and banana (Musa spp.). The antagonistic capacity of the six strains of T. asperellum was determined by evaluating the antibiosis at 48 h before the contact between both fungi and competition for space (diameter of the colonies and the percentage of inhibition of radial growth of the pathogen at 96 h) through the Dual Culture method. The metabolic activity of Trichoderma was variable on the growth of the isolates of the pathogen; however, the strain Ta.13 exerted inhibition on growth of both pathogens. The strains Ta.75, Ta.78, and Ta.90 of T. asperellum were located in class 1 of the Bell et al. scale, against the isolate of Colletotrichum spp. from banana, with the exception of the rest. Regarding the guava isolate, all strains were placed in class 2 of the scale. In general, all strains evaluated inhibited growth of pathogen isolates in more than 70 %, with respect to the control, although some strains stood out with more than 80 % inhibition of the pathogen growth. These results are of great importance from the practical point of view, since Colletotrichum causes great economic postharvest losses in tropical regions.El presente trabajo tuvo como objetivo evaluar el efecto antagónico de seis cepas de Trichoderma asperellum Samuels, Lieckfeldt & Nirenberg sobre dos aislamientos de Colletotrichum spp., obtenidos de plátano (Musa spp.) y guayaba (Psidium guajava L.). Para ello, se realizó el aislamiento del patógeno. La capacidad antagónica de seis cepas de T. asperellum se determinó evaluando la antibiosis a las 48 h antes del contacto entre ambos hongos y la competencia por espacio (diámetro de las colonias y el porcentaje de inhibición del crecimiento radial del patógeno, a las 96 h) a través de la técnica del Cultivo Dual. La actividad metabólica de Trichoderma fue variable sobre el crecimiento de los aislados del patógeno; no obstante, la cepa Ta.13 ejerció inhibición sobre el crecimiento de ambos patógenos. Las cepas Ta.75, Ta.78 y Ta.90 de T. asperellum se ubicaron en la clase 1 de la Escala de Bell et al., frente al aislado de Colletotrichum spp. de plátano, con excepción del resto. Mientras que, sobre el de guayaba, todas las cepas se ubicaron en la clase 2 de la escala. En general, todas las cepas evaluadas inhibieron en más de 70 % el crecimiento de los aislados del patógeno, con respecto al control; aunque se destacaron algunas con más de 80 % de inhibición del crecimiento de los patógenos. Estos resultados revisten gran importancia desde el punto de vista práctico, ya que Colletotrichum causa grandes pérdidas económicas en poscosecha, en regiones tropicales
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Efectos de Trichoderma asperellum Samuels, Lieckfeldt & Nirenberg y Azofert ® sobre el crecimiento y desarrollo de Phaseolus vulgaris L.
Centro Nacional de Sanidad Agropecuaria, 2021Co-Authors: González-marquetti Ivonne, Ynfante-martínez Danay, Gorrita Susana, Morales-mena Belkis, Nápoles, María Caridad, Delgado-oramas, Belkis Peteira, Martínez Coca BenedictoAbstract:The aim of this work was to evaluate the effect of Trichoderma asperellum Samuels, Lieckfeldt & Nirenberg (Ta. 13, Ta. 78 and Ta. 90) combined with Azofert® on growth and development of Phaseolus vulgaris L. (cv. 'BAT-304') under semi-controlled conditions. Evaluations were carried out in 32-day-old plants sown at two times: 1) plant sowing and soil inoculation with Trichoderma at the same time, 2) plant sowing seven days after soil inoculation with the fungus. The plant height, root length, number of leaves, number of flowers, number of tendrils and pods, fresh and dry weight of plant aerial parts and roots, Trichoderma endophytic capacity in roots, nodulation effectiveness by Rhizobium and the induction of peroxidase enzymes (POD), polyphenoloxidases (PPO), and phenylalanine ammonium lyases (PAL) were determined. All results were contrasted according to simple analysis of variance followed by the least significant difference test. The co-inoculation of Ta. 13 and Ta. 78 with Azofert® had a synergist effect on growth and defense enzymes stimulation of P. vulgaris. The ability of the T. asperellum strains studied to endophytically colonize the roots of P. vulgaris (cv. 'BAT-304') and their compatibility with Azofert® were verified, under controlled conditions. In general, the treatments to which T. asperellum was applied from the moment of sowing presented the best values of the growth and development parameters evaluated. The co-inoculation of Ta. 13 and Ta. 78 with Azofert® had a synergistic effect on growth stimulation and expression of defense enzymes PAL, PPO and POD of P. vulgaris.El objetivo de este trabajo fue evaluar el efecto combinado de tres cepas de Trichoderma asperellum Samuels, Lieckfeldt & Nirenberg (Ta. 13, Ta. 78 y Ta. 90) con Azofert® (Rhizobium leguminosarum cepa CF1) sobre el crecimiento y desarrollo de plantas de frijol común Phaseolus vulgaris L. (cv. 'BAT-304') en condiciones semicontroladas. Las evaluaciones se realizaron en plantas de 32 días, sembradas en dos momentos: 1) siembra e inoculación de Trichoderma en el suelo al unísono; 2: siembra de las plantas siete días después de la inoculación del suelo con el hongo. Se determinó la altura de las plantas, la longitud de la raíz, el número de hojas, flores, zarcillos y vainas, la masa fresca y seca del área foliar y radicular, la capacidad endófita de Trichoderma en las raíces, la efectividad de nodulación de Rhizobium y la inducción de enzimas peroxidasas (POD), polifenoloxidasas (PPO) y fenilalanina amonio liasas (PAL). Los resultados de los tratamientos, en ambas formas de inoculación, se contrastaron según análisis de varianza simple seguido de la prueba de mínima diferencia significativa. Se comprobó la capacidad de las cepas de T. asperellum estudiadas de colonizar endofíticamente las raíces de P. vulgaris (cv. 'BAT-304') y su compatibilidad con Azofert®, en condiciones controladas. De manera general, los tratamientos a los que se les aplicó T. asperellum desde el momento de la siembra presentaron los mejores valores de los parámetros de crecimiento y desarrollo evaluados. La coinoculación de las cepas Ta. 13 y Ta. 78 con Azofert® tuvo un efecto sinérgico sobre la estimulación del crecimiento y la expresión de enzimas de defensa PAL, PPO y POD de P. vulgaris
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Efecto de la aplicación conjunta de Pochonia chlamydosporia (Goddard) Zare y Gams y Trichoderma asperellum Samuels, Lieckfeldt y Nirenberg en vitroplantas de banano (Musa sp.)
Centro Nacional de Sanidad Agropecuaria, 2021Co-Authors: Arévalo Ortega Jersys, Martínez Coca Benedicto, Ynfante-martínez Danay, Hernández Socorro, Miguel A., Alfonso De La Cruz, Rolisbel, Hidalgo-díaz LeopoldoAbstract:The objective of this work was to evaluate the in vitro and ex vitro combination of P. chlamydosporia (IMI SD 187) and Trichoderma asperellum (Ta. 13) in the acclimatization of banana vitroplants Bluggoe type (AABB) ‘FHIA-03’. Firstly, the in vitro compatibility, through space competence (radial growth) and growth inhibition percentage at 24, 48, and 120 h, was assessed by the dual culture technique. In second place, it was evaluated the application of these fungi separately or in combination during the ex vitro adaptation phase of the vitroplants.. In total, 12 treatments were included, the treaments with each fungus and the control without fungus. The vitroplants were transplanted into polypropylene trays of 70 alveolus containing red ferralitic lixiviated soil and organic amendment (1:3 v/v) as substrate. P. chlamydosporia strain IMI SD 187 (KlamiC® Bionematicide) was applied with T. asperellum strain Ta.13 (SevetriC Biofungicide) by submerging the root before transplanting or by spraying the plant at 3 and 20 days post planting. The trays were randomly arranged in the adaptation area. At 30 days, the plant vegetative growth and the substrate and root colonization by P. chlamydosporia were assessed. In vitro, T. asperellum grew faster than P. chlamydosporia, but there was no direct hyphal contact between them. P. chlamydosporia colonized the root system and the rhizosphere of banana vitroplants when this fungus was applied separately or in combination with T. asperellum; both fungi promoted vitroplant growth. The results show that P. chlamydosporia and T. asperellum are good candidates for banana biomanagement strategies.El objetivo de este trabajo fue evaluar la combinación in vitro y ex vitro de P. chlamydosporia (IMI SD 187) y Trichoderma asperellum (Ta. 13) en la aclimatización de vitroplantas del banano de cocción del tipo Bluggoe (AABB) ‘FHIA-03’. Primeramente, se determinó la compatibilidad in vitro, mediante la competencia por espacio (crecimiento radial) y el porcentaje de inhibición del crecimiento a las 24, 48 y 120 h en Cultivo Dual. Posteriormente, se evaluó la aplicación de estos hongos de manera independiente o en combinación, durante la fase de adaptación ex vitro de vitroplantas, y un control sin aplicación; en total 12 tratamientos. Las vitroplantas se plantaron en bandejas de polipropileno de 70 alvéolos con sustrato: suelo ferralítico rojo lixiviado y cachaza (1:3 v/v). P. chlamydosporia cepa IMI SD 187 (Bionematicida KlamiC®) se aplicó con T. asperellum cepa Ta.13 (Biofungicida SevetriC) mediante inmersión de raíces previo al trasplante, o por aspersión a los 3 y 20 días posteriores al trasplante. Las bandejas se distribuyeron al azar en el área de aclimatización. A los 30 días, se evaluó el crecimiento de las vitroplantas y la colonización de P. chlamydosporia en el sustrato y las raíces. In vitro, T. asperellum creció más rápido que P. chlamydosporia, pero no hubo contacto hifal directo entre ambos hongos. P. chlamydosporia colonizó el sistema radical y la rizosfera de las vitroplantas cuando se aplicó de forma independiente y en combinación con T. asperellum y ambos hongos promovieron el crecimiento vegetal. Los resultados indican que P. chlamydosporia y T. asperellum son buenos candidatos para estrategias de biomanejo en banano
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Trichoderma asperellum Samuels, Lieckfeldt & Nirenberg cepa MV 020013 - promisorio agente para el manejo de Fusarium oxysporum cubense R4T
Centro Nacional de Sanidad Agropecuaria, 2020Co-Authors: Martínez Coca Benedicto, Fernando Torres Luis, Pineda MiltonAbstract:Dear editor:The Tropical Race 4 (TR4) of Fusarium oxysporum f. sp. cubense (FOC) is a threat to the production of plantains and bananas in Latin America due to its devastating effect and difficult control. Based on these antecedents, the strain MV 020013 of Trichoderma asperellum Samuels, Lieckfeldt & Nirenberg (Ta.13), from the National Center for Plant and Animal Health (CENSA) in Cuba, was evaluated against a FOC-TR4 isolate at Real Strong AgriCulture Innovention, in the Philippines. The dual-culture technique was used in a PDA medium at 28°C for 12 days. The inhibition percentage of pathogen growth on the co-culture was calculated and compared with that of the control without the antagonist. This strain of T. asperellum showed high efficacy in controlling this pathogen. At the sixth day of culture, the percentage of FOC TR4 inhibition was 55.60 %, and, on day 12, the antagonist strain had overgrown the Fusarium colony and completely inhibited its growth. This makes evident the presence of mycoparasitism and the possible release of toxic metabolites to the plant pathogen by the antagonist. This result shows the potential of the strain Ta. 13 of T. asperellum against the causal agent of Panama Disease TR4, being a promising strain that should be evaluated to determine the form of application, dose and efficacy in the control of this disease under field conditions.Sincerely, Dear editor:The Tropical Race 4 (TR4) of Fusarium oxysporum f. sp. cubense (FOC) is a threat to the production of plantains and bananas in Latin America due to its devastating effect and difficult control. Based on these antecedents, the strain MV 020013 of Trichoderma asperellum Samuels, Lieckfeldt & Nirenberg (Ta.13), from the National Center for Plant and Animal Health (CENSA) in Cuba, was evaluated against a FOC-TR4 isolate at Real Strong AgriCulture Innovention, in the Philippines. The dual-culture technique was used in a PDA medium at 28°C for 12 days. The inhibition percentage of pathogen growth on the co-culture was calculated and compared with that of the control without the antagonist. This strain of T. asperellum showed high efficacy in controlling this pathogen. At the sixth day of culture, the percentage of FOC TR4 inhibition was 55.60 %, and, on day 12, the antagonist strain had overgrown the Fusarium colony and completely inhibited its growth. This makes evident the presence of mycoparasitism and the possible release of toxic metabolites to the plant pathogen by the antagonist. This result shows the potential of the strain Ta. 13 of T. asperellum against the causal agent of Panama Disease TR4, being a promising strain that should be evaluated to determine the form of application, dose and efficacy in the control of this disease under field conditions.Sincerely,
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Evaluación in vitro de cepas de Trichoderma asperellum Samuels, Lieckfeldt & Nirenberg como promisorios agentes para el control de Alternaria solani Sorauer
Centro Nacional de Sanidad Agropecuaria, 2020Co-Authors: Martínez Coca Benedicto, Ronnie Gakenge Edwin, Miranda Cabrera IleanaAbstract:The aim of this work was to evaluate in vitro antagonism of 13 Cuban Trichoderma asperellum Samuels, Lieckfeldt & Nirenberg (Ta.) strains against Alternaria solani Sorauer. The modes of action of T. asperellum strains against A. solani in dual culture were determined. The results showed differences between the antagonist strains. The strains Ta. 90, Ta. 78, Ta. 75, and Ta. 85 were highlighted by their antibiotic effect. In the space competition, only Ta. 85 colonized 100 % of the pathogen (Bell et al. scale Grade 1.), while Ta. 17, Ta. 3, Ta. 90, Ta. 78, and Ta. 28 had more than 80 % growth over A. solani (Grade 2 of the scale). However, Ta. 75, Ta. 78, Ta. 79, and Ta. 85 strains proved to be good space competitors against the pathogen, with more than 50 % inhibition of radial growth of the pathogen. The strains Ta. 13, Ta. 56 and Ta. 85 had four hyphal interactions with A. solani; the most observed were vacuolization, penetration and granulation. The strains Ta. 1, Ta. 3, Ta. 12, Ta. 13, Ta. 25, Ta. 28, Ta. 56 and Ta. 85 caused significant reduction of the area occupied by the pathogen at 400 h, relative to the size at 96 h. The strains Ta. 85, Ta. 1 and Ta. 12 were highlighted in this action; the strain Ta. 85 was selected as the most promising; however, Ta. 28, Ta. 56 and Ta. 13 showed good qualities for evaluation in field conditions.El objetivo de este trabajo fue evaluar el antagonismo in vitro de 13 cepas cubanas de Trichoderma asperellum Samuels, Lieckfeldt & Nirenberg frente Alternaria solani Sorauer. Para ello se determinaron los modos de acción de las cepas de T. asperellum frente a A. solani en cultivo dual. Los resultados mostraron diferencias entre las cepas del antagonista. En la antibiosis se destacaron las cepas Ta. 90, Ta. 78, Ta. 75 y Ta. 85. En la competencia por espacio, solo la Ta. 85 colonizó el 100 % del patógeno (grado 1 de la escala de Bell et al.), mientras que Ta. 17, Ta. 3, Ta. 90, Ta. 78 y Ta. 28 tuvieron un crecimiento mayor al 80 % sobre A. solani, grado 2 de la escala. No obstante, las cepas Ta. 75, Ta. 78, Ta. 79 y Ta. 85 mostraron ser buenas competidoras por espacio frente al patógeno, con más de 50 % de inhibición del crecimiento radial del mismo. Las cepas Ta. 13, Ta. 56 y Ta. 85 presentaron cuatro interacciones hifales frente a A. solani. Las más observadas fueron la vacuolización, penetración y granulación. Las cepas Ta. 1, Ta. 3, Ta. 12, Ta. 13, Ta. 25, Ta. 28, Ta. 56 y Ta. 85 provocaron reducción significativa del área ocupada por el patógeno a las 400 h, con relación al tamaño a las 96 h. En esta acción sobresalieron las cepas Ta. 85, Ta 1 y Ta 12; la cepa Ta.85 se seleccionó como la más promisoria. No obstante, Ta.28, Ta. 56 y Ta. 13 mostraron buenas cualidades para su evaluación en condiciones de campo
Xiu-li Yin - One of the best experts on this subject based on the ideXlab platform.
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trichobisabolins a h eight new bisabolane derivatives from the marine alga epiphytic fungus Trichoderma asperellum y6 2
Fitoterapia, 2019Co-Authors: Zhenzhen Shi, Feng-ping Miao, Sheng-tao Fang, Xiu-li YinAbstract:Abstract Eight new bisabolane derivatives, trichobisabolins A-H, along with two known ones, (3R,6R,7R)-1,10-bisaboladien-3-ol (9) and (3R,6R,7S)-1,10-bisaboladien-3,6-diol (10) were isolated from the culture of Trichoderma asperellum Y6–2, obtained from the surface of the marine red alga Chondrus ocellatus. Their structures and relative configurations were identified by interpretation of 1D/2D NMR and MS data. Compounds 1–8 were assayed for inhibiting the growth of some marine-derived organisms, including four marine phytoplankton species, one marine zooplankton species, and five pathogenic bacteria. All of them exhibited inhibition against the marine phytoplanktons with IC50 values ranging from 2.1–78 μg/mL, compounds 4 and 8 showed weak lethality to the marine zooplankton, and none of them had inhibition against the five pathogenic bacteria.
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Cyclonerane Derivatives from the Algicolous Endophytic Fungus Trichoderma asperellum A-YMD-9-2
MDPI AG, 2019Co-Authors: Yin-ping Song, Feng-ping Miao, Xianghong Liu, Xiu-li YinAbstract:Seven previously unreported cyclonerane derivatives, namely, 3,7,11-trihydroxycycloneran-10-one, cycloneran-3,7,10,11-tetraol, cycloneran-3,7,11-triol, 11,12,15-trinorcycloneran-3,7,10-triol, 7,10S-epoxycycloneran-3,15-diol, 7,10R-epoxycycloneran-3,15-diol, and (10Z)-15-acetoxy-10-cycloneren-3,7-diol, were isolated in addition to the known (10Z)-cyclonerotriol, (10E)-cyclonerotriol, catenioblin C, and chokol E from the culture of Trichoderma asperellum A-YMD-9-2, an endophytic fungus obtained from the marine red alga Gracilaria verrucosa. The structures of previously unreported compounds were established by spectroscopic techniques, including 1D/2D NMR, MS, and IR. The isolation of these new cyclonerane derivatives greatly adds to the structural diversity of unusual cyclonerane sesquiterpenes, and several isolates exhibit potent inhibition against some marine phytoplankton species
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tricholumin a a highly transformed ergosterol derivative from the alga endophytic fungus Trichoderma asperellum
Organic Letters, 2018Co-Authors: Yin-ping Song, Feng-ping Miao, Sheng-tao Fang, Zhenzhen Shi, Xiu-li YinAbstract:Tricholumin A (1) with an unprecedented carbon skeleton was isolated from the fungus Trichoderma asperellum cf44-2, an endophyte from the marine brown alga Sargassum sp. Its structure and relative configuration were identified by extensive 1D/2D NMR and mass spectrometric data, and the absolute configuration was assigned by X-ray diffraction and ECD calculations. Compound 1 represents a highly transformed ergosterol derivative, and it exhibited inhibition of some pathogenic microbes and marine phytoplankton species.
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Halogenated and Nonhalogenated Metabolites from the Marine-Alga-Endophytic Fungus Trichoderma asperellum cf44-2
MDPI AG, 2018Co-Authors: Yin-ping Song, Feng-ping Miao, Sheng-tao Fang, Xiu-li YinAbstract:One new bisabolane sesquiterpene, bisabolan-1,10,11-triol (1), one new norbisabolane sesquiterpene, 12-nor-11-acetoxybisabolen-3,6,7-triol (2), two new naturally occurring monoterpenes, (7S)- and (7R)-1-hydroxy-3-p-menthen-9-oic acids (3 and 4), one new naturally occurring trichodenone, dechlorotrichodenone C (5), one new chlorine-containing trichodenone, 3-hydroxytrichodenone C (6), one new diketopiperazine, methylcordysinin A (7), and one new naturally occurring oxazole derivative, 4-oxazolepropanoic acid (8), were isolated from the culture of a marine brown alga-endophytic strain (cf44-2) of Trichoderma asperellum. Their structures and relative configurations were determined by extensive 1D/2D NMR and mass spectrometric data, and the absolute configurations of 3–6 were assigned by analysis of the ECD spectra aided by quantum chemical computations. Compounds 1, 2, 5, and 6 showed growth inhibition of some marine phytoplankton species and pathogenic bacteria
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trichobisabolins a h eight new bisabolane derivatives from the marine alga epiphytic fungus Trichoderma asperellum y6 2
Fitoterapia, 2019Co-Authors: Zhenzhen Shi, Feng-ping Miao, Sheng-tao Fang, Xiu-li YinAbstract:Abstract Eight new bisabolane derivatives, trichobisabolins A-H, along with two known ones, (3R,6R,7R)-1,10-bisaboladien-3-ol (9) and (3R,6R,7S)-1,10-bisaboladien-3,6-diol (10) were isolated from the culture of Trichoderma asperellum Y6–2, obtained from the surface of the marine red alga Chondrus ocellatus. Their structures and relative configurations were identified by interpretation of 1D/2D NMR and MS data. Compounds 1–8 were assayed for inhibiting the growth of some marine-derived organisms, including four marine phytoplankton species, one marine zooplankton species, and five pathogenic bacteria. All of them exhibited inhibition against the marine phytoplanktons with IC50 values ranging from 2.1–78 μg/mL, compounds 4 and 8 showed weak lethality to the marine zooplankton, and none of them had inhibition against the five pathogenic bacteria.
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tricholumin a a highly transformed ergosterol derivative from the alga endophytic fungus Trichoderma asperellum
Organic Letters, 2018Co-Authors: Yin-ping Song, Feng-ping Miao, Sheng-tao Fang, Zhenzhen Shi, Xiu-li YinAbstract:Tricholumin A (1) with an unprecedented carbon skeleton was isolated from the fungus Trichoderma asperellum cf44-2, an endophyte from the marine brown alga Sargassum sp. Its structure and relative configuration were identified by extensive 1D/2D NMR and mass spectrometric data, and the absolute configuration was assigned by X-ray diffraction and ECD calculations. Compound 1 represents a highly transformed ergosterol derivative, and it exhibited inhibition of some pathogenic microbes and marine phytoplankton species.
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bisabolane cyclonerane and harziane derivatives from the marine alga endophytic fungus Trichoderma asperellum cf44 2
Phytochemistry, 2018Co-Authors: Yin-ping Song, Feng-ping Miao, Xianghong Liu, Zhenzhen Shi, Sheng-tao FangAbstract:Three undescribed bisabolane derivatives, trichaspin, trichaspsides A and B, three undescribed cyclonerane sesquiterpenes, 9-cycloneren-3,7,11-triol, 11-cycloneren-3,7,10-triol, and 7,10-epoxycycloneran-3,11,12-triol, and one undescribed harziane diterpene, 11-hydroxy-9-harzien-3-one, were obtained from the culture of Trichoderma asperellum cf44-2, an endophyte of the marine brown alga Sargassum sp. Their structures and relative configurations were assigned by analysis of 1D/2D NMR and MS data, and their absolute configurations were established by ECD or specific optical rotation data. Trichaspin features an unprecedented ethylated bisabolane skeleton, while trichaspsides A and B represent the first aminoglycosides of bisabolane and norbisabolane sesquiterpenes, respectively. Nine of the compounds were evaluated for inhibition of five marine-derived pathogenic bacteria and toxicity to a marine zooplankton.
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Halogenated and Nonhalogenated Metabolites from the Marine-Alga-Endophytic Fungus Trichoderma asperellum cf44-2
MDPI AG, 2018Co-Authors: Yin-ping Song, Feng-ping Miao, Sheng-tao Fang, Xiu-li YinAbstract:One new bisabolane sesquiterpene, bisabolan-1,10,11-triol (1), one new norbisabolane sesquiterpene, 12-nor-11-acetoxybisabolen-3,6,7-triol (2), two new naturally occurring monoterpenes, (7S)- and (7R)-1-hydroxy-3-p-menthen-9-oic acids (3 and 4), one new naturally occurring trichodenone, dechlorotrichodenone C (5), one new chlorine-containing trichodenone, 3-hydroxytrichodenone C (6), one new diketopiperazine, methylcordysinin A (7), and one new naturally occurring oxazole derivative, 4-oxazolepropanoic acid (8), were isolated from the culture of a marine brown alga-endophytic strain (cf44-2) of Trichoderma asperellum. Their structures and relative configurations were determined by extensive 1D/2D NMR and mass spectrometric data, and the absolute configurations of 3–6 were assigned by analysis of the ECD spectra aided by quantum chemical computations. Compounds 1, 2, 5, and 6 showed growth inhibition of some marine phytoplankton species and pathogenic bacteria
