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Cletus P Kurtzman - One of the best experts on this subject based on the ideXlab platform.
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Blastobotrys americana sp. nov., Blastobotrys illinoisensis sp. nov., Blastobotrys malaysiensis sp. nov., Blastobotrys muscicola sp. nov., Blastobotrys peoriensis sp. nov. and Blastobotrys raffinosifermentans sp. nov., novel anamorphic yeast species.
International journal of systematic and evolutionary microbiology, 2020Co-Authors: Cletus P KurtzmanAbstract:The genus Blastobotrys, which now includes species previously assigned to the synonymous genera Arxula and Sympodiomyces, represents the anamorph of the ascosporogenous genus Trichomonascus. Six novel species are proposed for assignment to Blastobotrys. They were detected from their unique nucleotide sequences in large-subunit rDNA, ITS1-5.8S-ITS2 rDNA, mitochondrial small-subunit rDNA and the cytochrome oxidase II gene. The proposed novel species are Blastobotrys americana sp. nov. (type strain NRRL Y-6844(T)=CBS 10337(T); substrate unknown; Kansas, USA), Blastobotrys illinoisensis sp. nov. (type strain NRRL YB-1343(T)=CBS 10339(T); from forest debris; Illinois, USA), Blastobotrys malaysiensis sp. nov. (type strain NRRL Y-6417(T)=CBS 10336(T); from soil; Malaysia), Blastobotrys muscicola sp. nov. (type strain NRRL Y-7993(T)=CBS 10338(T); from moss; Louisiana, USA), Blastobotrys peoriensis sp. nov. (type strain NRRL YB-2290(T)=CBS 10340(T); from a fungus; Peoria, IL, USA) and Blastobotrys raffinosifermentans sp. nov. (type strain NRRL Y-27150(T)=CBS 6800(T); substrate unknown).
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glucosylation and other biotransformations of t 2 toxin by yeasts of the Trichomonascus clade
Applied and Environmental Microbiology, 2012Co-Authors: Susan P Mccormick, Neil P J Price, Cletus P KurtzmanAbstract:Trichothecenes are sesquiterpenoid toxins produced by Fusarium species. Since these mycotoxins are very stable, there is interest in microbial transformations that can remove toxins from contaminated grain or cereal products. Twenty-three yeast species assigned to the Trichomonascus clade (Saccharomycotina, Ascomycota), including four Trichomonascus species and 19 anamorphic species presently classified in Blastobotrys, were tested for their ability to convert the trichothecene T-2 toxin to less-toxic products. These species gave three types of biotransformations: acetylation to 3-acetyl T-2 toxin, glycosylation to T-2 toxin 3-glucoside, and removal of the isovaleryl group to form neosolaniol. Some species gave more than one type of biotransformation. Three Blastobotrys species converted T-2 toxin into T-2 toxin 3-glucoside, a compound that has been identified as a masked mycotoxin in Fusarium-infected grain. This is the first report of a microbial whole-cell method for producing trichothecene glycosides, and the potential large-scale availability of T-2 toxin 3-glucoside will facilitate toxicity testing and development of methods for detection of this compound in agricultural and other products.
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Relationships among genera of the Saccharomycotina (Ascomycota) from multigene phylogenetic analysis of type species
Fems Yeast Research, 2012Co-Authors: Cletus P Kurtzman, Christie J RobnettAbstract:Relationships among ascomycetous yeast genera (subphylum Saccharomycotina, phylum Ascomycota) have been uncertain. In the present study, type species of 70 currently recognized genera are compared from divergence in the nearly entire nuclear gene sequences for large subunit rRNA, small subunit (SSU) rRNA, translation elongation factor-1α, and RNA polymerase II, subunits 1 (RPB1) and 2 (RPB2). The analysis substantiates earlier proposals that all known ascomycetous yeast genera now assigned to the Saccharomycotina represent a single clade. Maximum likelihood analysis resolved the taxa into eight large multigenus clades and four-one- and two-genus clades. Maximum parsimony and neighbor-joining analyses gave similar results. Genera of the family Saccharomycetaceae remain as one large clade as previously demonstrated, to which the genus Cyniclomyces is now assigned. Pichia, Saturnispora, Kregervanrija, Dekkera, Ogataea and Ambrosiozyma are members of a single large clade, which is separate from the clade that includes Barnettozyma, Cyberlindnera, Phaffomyces, Starmera and Wickerhamomyces. Other clades include Kodamaea, Metschnikowia, Debaryomyces, Cephaloascus and related genera, which are separate from the clade that includes Zygoascus, Trichomonascus, Yarrowia and others. This study once again demonstrates that there is limited congruence between a system of classification based on phenotype and a system determined from DNA sequences.
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Diddensiella caesifluorescens gen. nov., sp. nov., a riboflavin-producing yeast species of the family Trichomonascaceae.
International journal of systematic and evolutionary microbiology, 2012Co-Authors: Gabor Peter, Denes Dlauchy, Neil P J Price, Cletus P KurtzmanAbstract:Four strains of a novel heterothallic yeast species were isolated from rotten wood collected in or near the Pilis Mountains in Hungary. The strains produced riboflavin in liquid culture. Analysis of gene sequences for the D1/D2 domains of the LSU nuclear rRNA, as well as analysis of concatenated gene sequences for the D1/D2 nuclear LSU rRNA, mitochondrial SSU rRNA and cytochrome oxidase II placed the novel species in a small clade including only two recognized species, Candida santjacobensis and Candida transvaalensis, in the family Trichomonascaceae. DNA sequence analyses demonstrated that the novel species was distinct from all currently recognized teleomorphic yeast genera. The name Diddensiella caesifluorescens gen nov., sp. nov. is proposed to accommodate the novel genus and species. The new genus proposed here can be recognized only from gene sequence analysis, because the characters of its asexual reproduction and ascospore formation are shared by several members of the genera Trichomonascus, Sugiyamaella and Spencermartinsiella. The type and isotype strains of D. caesifluorescens are NCAIM Y.01949(T) ( = NRRL Y-48781(T) = CBS 12613(T)) and NCAIM Y.01956(I) ( = NRRL Y-48782(I) = CBS 12614(I)), respectively. In view of their close relatedness to D. caesifluorescens, C. santjacobensis and C. transvaalensis are transferred to the genus Diddensiella as new combinations in accordance with changes in the International Code of Nomenclature for algae, fungi and plants.
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Trichomonascus H.S. Jackson emend. Kurtzman & Robnett (2007)
The Yeasts, 2011Co-Authors: Maudy Th. Smith, G. Sybren De Hoog, David Malloch, Cletus P KurtzmanAbstract:Publisher Summary This chapter studies the genus Trichomonascus. In the determination of the asexual reproduction it is seen that colonies are often dry in texture and tannish-white. Asexual reproduction is by multilateral budding and the formation of pseudohyphae and true hyphae, which often produce short chains of blastoconidia that form sympodially. Septa have micropores.The anamorph genus is Blastobotrys. In sexual reproduction it is found that the species may be heterothallic or homothallic. Gametangia may form on hyphal tips or on hyphae after fusion of two intercalary cells by lateral outgrowths. Asci are globose to ellipsoidal with firm, thick walls and contain two to four ascospores. Ascospores are long-ellipsoidal to fusiform or hat- or helmet-shaped. Asci may bear a sterile apical cell. The chapter also discusses physiology/biochemistry and phylogenetic placement of the genus, in which sugars may be fermented, nitrate is not assimilated, and the diazonium blue B reaction is negative. The type species taken is Trichomonascus mycophagus. The fungus is unusual because it is characterized by an ascus that develops from a hyphal tip and is soon accompanied by a slender “trichogyne-like” hypha that arises from the basal cell supporting the ascus. The tip of the trichogyne-like hypha fuses with the top of the ascus, giving the appearance of having completed a fertilization process. The hypha and the apex of the ascus, which becomes a separate cell, broaden and fuse to form an inverted, lipid-filled, claw-like structure that partially enclosed the ascus.
Denes Dlauchy - One of the best experts on this subject based on the ideXlab platform.
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Trichomonascus apis sp. nov., a heterothallic yeast species from honeycomb.
International journal of systematic and evolutionary microbiology, 2020Co-Authors: Gabor Peter, Judit Tornai-lehoczki, Denes DlauchyAbstract:Four strains of a novel heterothallic yeast species were isolated from pollen-storing cells of a honeycomb of honeybee (Apis mellifera) in Hungary. Analysis of the D1/D2 domain of the large-subunit (26S) rRNA gene sequences placed the strains in the Trichomonascus clade. The four strains share identical D1/D2 sequences and differ by 24 substitutions and nine indels from the genetically most closely related species, Blastobotrys attinorum. The name Trichomonascus apis sp. nov. is proposed for the novel species. The carbon-source assimilation spectrum of T. apis sp. nov. is rather broad. Unlike B. attinorum, it assimilates sucrose, trehalose, d-glucuronate and succinate and does not grow at 37 degrees C, thus enabling the two taxa to be distinguished. The type and isotype strains of Trichomonascus apis are NCAIM Y.01848(T) (=CBS 10922(T) =NRRL Y-48475(T)) and NCAIM Y.01849(IT) (=CBS 10923(IT) =NRRL Y-48476(IT)), respectively.
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Diddensiella caesifluorescens gen. nov., sp. nov., a riboflavin-producing yeast species of the family Trichomonascaceae.
International journal of systematic and evolutionary microbiology, 2012Co-Authors: Gabor Peter, Denes Dlauchy, Neil P J Price, Cletus P KurtzmanAbstract:Four strains of a novel heterothallic yeast species were isolated from rotten wood collected in or near the Pilis Mountains in Hungary. The strains produced riboflavin in liquid culture. Analysis of gene sequences for the D1/D2 domains of the LSU nuclear rRNA, as well as analysis of concatenated gene sequences for the D1/D2 nuclear LSU rRNA, mitochondrial SSU rRNA and cytochrome oxidase II placed the novel species in a small clade including only two recognized species, Candida santjacobensis and Candida transvaalensis, in the family Trichomonascaceae. DNA sequence analyses demonstrated that the novel species was distinct from all currently recognized teleomorphic yeast genera. The name Diddensiella caesifluorescens gen nov., sp. nov. is proposed to accommodate the novel genus and species. The new genus proposed here can be recognized only from gene sequence analysis, because the characters of its asexual reproduction and ascospore formation are shared by several members of the genera Trichomonascus, Sugiyamaella and Spencermartinsiella. The type and isotype strains of D. caesifluorescens are NCAIM Y.01949(T) ( = NRRL Y-48781(T) = CBS 12613(T)) and NCAIM Y.01956(I) ( = NRRL Y-48782(I) = CBS 12614(I)), respectively. In view of their close relatedness to D. caesifluorescens, C. santjacobensis and C. transvaalensis are transferred to the genus Diddensiella as new combinations in accordance with changes in the International Code of Nomenclature for algae, fungi and plants.
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Trichomonascus apis sp nov a heterothallic yeast species from honeycomb
International Journal of Systematic and Evolutionary Microbiology, 2009Co-Authors: Gabor Peter, Judit Tornailehoczki, Denes DlauchyAbstract:Four strains of a novel heterothallic yeast species were isolated from pollen-storing cells of a honeycomb of honeybee (Apis mellifera) in Hungary. Analysis of the D1/D2 domain of the large-subunit (26S) rRNA gene sequences placed the strains in the Trichomonascus clade. The four strains share identical D1/D2 sequences and differ by 24 substitutions and nine indels from the genetically most closely related species, Blastobotrys attinorum. The name Trichomonascus apis sp. nov. is proposed for the novel species. The carbon-source assimilation spectrum of T. apis sp. nov. is rather broad. Unlike B. attinorum, it assimilates sucrose, trehalose, d-glucuronate and succinate and does not grow at 37 °C, thus enabling the two taxa to be distinguished. The type and isotype strains of Trichomonascus apis are NCAIM Y.01848T (=CBS 10922T =NRRL Y-48475T) and NCAIM Y.01849IT (=CBS 10923IT =NRRL Y-48476IT), respectively.
Gabor Peter - One of the best experts on this subject based on the ideXlab platform.
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Trichomonascus apis sp. nov., a heterothallic yeast species from honeycomb.
International journal of systematic and evolutionary microbiology, 2020Co-Authors: Gabor Peter, Judit Tornai-lehoczki, Denes DlauchyAbstract:Four strains of a novel heterothallic yeast species were isolated from pollen-storing cells of a honeycomb of honeybee (Apis mellifera) in Hungary. Analysis of the D1/D2 domain of the large-subunit (26S) rRNA gene sequences placed the strains in the Trichomonascus clade. The four strains share identical D1/D2 sequences and differ by 24 substitutions and nine indels from the genetically most closely related species, Blastobotrys attinorum. The name Trichomonascus apis sp. nov. is proposed for the novel species. The carbon-source assimilation spectrum of T. apis sp. nov. is rather broad. Unlike B. attinorum, it assimilates sucrose, trehalose, d-glucuronate and succinate and does not grow at 37 degrees C, thus enabling the two taxa to be distinguished. The type and isotype strains of Trichomonascus apis are NCAIM Y.01848(T) (=CBS 10922(T) =NRRL Y-48475(T)) and NCAIM Y.01849(IT) (=CBS 10923(IT) =NRRL Y-48476(IT)), respectively.
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Diddensiella caesifluorescens gen. nov., sp. nov., a riboflavin-producing yeast species of the family Trichomonascaceae.
International journal of systematic and evolutionary microbiology, 2012Co-Authors: Gabor Peter, Denes Dlauchy, Neil P J Price, Cletus P KurtzmanAbstract:Four strains of a novel heterothallic yeast species were isolated from rotten wood collected in or near the Pilis Mountains in Hungary. The strains produced riboflavin in liquid culture. Analysis of gene sequences for the D1/D2 domains of the LSU nuclear rRNA, as well as analysis of concatenated gene sequences for the D1/D2 nuclear LSU rRNA, mitochondrial SSU rRNA and cytochrome oxidase II placed the novel species in a small clade including only two recognized species, Candida santjacobensis and Candida transvaalensis, in the family Trichomonascaceae. DNA sequence analyses demonstrated that the novel species was distinct from all currently recognized teleomorphic yeast genera. The name Diddensiella caesifluorescens gen nov., sp. nov. is proposed to accommodate the novel genus and species. The new genus proposed here can be recognized only from gene sequence analysis, because the characters of its asexual reproduction and ascospore formation are shared by several members of the genera Trichomonascus, Sugiyamaella and Spencermartinsiella. The type and isotype strains of D. caesifluorescens are NCAIM Y.01949(T) ( = NRRL Y-48781(T) = CBS 12613(T)) and NCAIM Y.01956(I) ( = NRRL Y-48782(I) = CBS 12614(I)), respectively. In view of their close relatedness to D. caesifluorescens, C. santjacobensis and C. transvaalensis are transferred to the genus Diddensiella as new combinations in accordance with changes in the International Code of Nomenclature for algae, fungi and plants.
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Trichomonascus apis sp nov a heterothallic yeast species from honeycomb
International Journal of Systematic and Evolutionary Microbiology, 2009Co-Authors: Gabor Peter, Judit Tornailehoczki, Denes DlauchyAbstract:Four strains of a novel heterothallic yeast species were isolated from pollen-storing cells of a honeycomb of honeybee (Apis mellifera) in Hungary. Analysis of the D1/D2 domain of the large-subunit (26S) rRNA gene sequences placed the strains in the Trichomonascus clade. The four strains share identical D1/D2 sequences and differ by 24 substitutions and nine indels from the genetically most closely related species, Blastobotrys attinorum. The name Trichomonascus apis sp. nov. is proposed for the novel species. The carbon-source assimilation spectrum of T. apis sp. nov. is rather broad. Unlike B. attinorum, it assimilates sucrose, trehalose, d-glucuronate and succinate and does not grow at 37 °C, thus enabling the two taxa to be distinguished. The type and isotype strains of Trichomonascus apis are NCAIM Y.01848T (=CBS 10922T =NRRL Y-48475T) and NCAIM Y.01849IT (=CBS 10923IT =NRRL Y-48476IT), respectively.
Christie J Robnett - One of the best experts on this subject based on the ideXlab platform.
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Relationships among genera of the Saccharomycotina (Ascomycota) from multigene phylogenetic analysis of type species
Fems Yeast Research, 2012Co-Authors: Cletus P Kurtzman, Christie J RobnettAbstract:Relationships among ascomycetous yeast genera (subphylum Saccharomycotina, phylum Ascomycota) have been uncertain. In the present study, type species of 70 currently recognized genera are compared from divergence in the nearly entire nuclear gene sequences for large subunit rRNA, small subunit (SSU) rRNA, translation elongation factor-1α, and RNA polymerase II, subunits 1 (RPB1) and 2 (RPB2). The analysis substantiates earlier proposals that all known ascomycetous yeast genera now assigned to the Saccharomycotina represent a single clade. Maximum likelihood analysis resolved the taxa into eight large multigenus clades and four-one- and two-genus clades. Maximum parsimony and neighbor-joining analyses gave similar results. Genera of the family Saccharomycetaceae remain as one large clade as previously demonstrated, to which the genus Cyniclomyces is now assigned. Pichia, Saturnispora, Kregervanrija, Dekkera, Ogataea and Ambrosiozyma are members of a single large clade, which is separate from the clade that includes Barnettozyma, Cyberlindnera, Phaffomyces, Starmera and Wickerhamomyces. Other clades include Kodamaea, Metschnikowia, Debaryomyces, Cephaloascus and related genera, which are separate from the clade that includes Zygoascus, Trichomonascus, Yarrowia and others. This study once again demonstrates that there is limited congruence between a system of classification based on phenotype and a system determined from DNA sequences.
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multigene phylogenetic analysis of the Trichomonascus wickerhamiella and zygoascus yeast clades and the proposal of sugiyamaella gen nov and 14 new species combinations
Fems Yeast Research, 2007Co-Authors: Cletus P Kurtzman, Christie J RobnettAbstract:Relationships among species assigned to the ascosporic yeast genera Sporopachydermia , Stephanoascus , Trichomonascus , Wickerhamiella and Zygoascus , and to the associated anamorphic genera Arxula , Blastobotrys , Sympodiomyces and Trigonopsis , were determined from phylogenetic analyses of gene sequences from the nearly complete large-subunit rRNA gene, the mitochondrial small-subunit rRNA gene, and cytochrome oxidase II. The genus Stephanoascus is polyphyletic, resulting in reassignment of two species to the older genus Trichomonascus and the third to Sugiyamaella gen. nov. (type species Sugiyamaella smithiae ). The genera Sporopachydermia , Wickerhamiella and Zygoascus appear to be monophyletic. The species Pichia ofunaensis and P. tannicola are proposed for transfer to Zygoascus . Arxula , Blastobotrys and Sympodiomyces are members of the Trichomonascus clade, with the genus Blastobotrys having taxonomic priority for anamorphic states. Trigonopsis variabilis and three species of Candida represent a distinct clade. From the foregoing gene sequence analyses, the new ascosporic genus Sugiyamaella is proposed, as are 14 new species combinations and the new family Trichomonascaceae .
Judit Tornailehoczki - One of the best experts on this subject based on the ideXlab platform.
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Trichomonascus apis sp nov a heterothallic yeast species from honeycomb
International Journal of Systematic and Evolutionary Microbiology, 2009Co-Authors: Gabor Peter, Judit Tornailehoczki, Denes DlauchyAbstract:Four strains of a novel heterothallic yeast species were isolated from pollen-storing cells of a honeycomb of honeybee (Apis mellifera) in Hungary. Analysis of the D1/D2 domain of the large-subunit (26S) rRNA gene sequences placed the strains in the Trichomonascus clade. The four strains share identical D1/D2 sequences and differ by 24 substitutions and nine indels from the genetically most closely related species, Blastobotrys attinorum. The name Trichomonascus apis sp. nov. is proposed for the novel species. The carbon-source assimilation spectrum of T. apis sp. nov. is rather broad. Unlike B. attinorum, it assimilates sucrose, trehalose, d-glucuronate and succinate and does not grow at 37 °C, thus enabling the two taxa to be distinguished. The type and isotype strains of Trichomonascus apis are NCAIM Y.01848T (=CBS 10922T =NRRL Y-48475T) and NCAIM Y.01849IT (=CBS 10923IT =NRRL Y-48476IT), respectively.
