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V G Sukumaran - One of the best experts on this subject based on the ideXlab platform.
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evaluation of antimicrobial efficacy of herbal alternatives Triphala and green tea polyphenols mtad and 5 sodium hypochlorite against enterococcus faecalis biofilm formed on tooth substrate an in vitro study
Journal of Endodontics, 2010Co-Authors: J Prabhakar, M Senthilkumar, M S Priya, K Mahalakshmi, P K Sehgal, V G SukumaranAbstract:Abstract Introduction The purpose of this study was to evaluate the antimicrobial efficacy of Triphala, green tea polyphenols (GTP), MTAD, and 5% sodium hypochlorite against E. faecalis biofilm formed on tooth substrate. Methods Extracted human teeth were biomechanically prepared, vertically sectioned, placed in the tissue culture wells exposing the root canal surface to E. faecalis to form a biofilm. At the end of the 3rd and 6th weeks all groups were treated for 10 minutes with the test solutions and control and were analyzed qualitatively and quantitatively. Results Qualitative assay with 3-week biofilm showed complete inhibition of bacterial growth with Triphala, MTAD and NaOCl, except GTP and saline, which showed presence of bacterial growth. In quantitative analysis, GTP- and saline-treated tooth samples have shown 1516 ± 17.2 CFU/mL, 156.4 × 10 9 ± 3.1 × 10 9 CFU/mL respectively. Qualitative assay with 6-week biofilm showed growth when treated with Triphala, GTP and MTAD whereas NaOCl has shown complete inhibition. All groups except NaOCl showed eight log reduction when compared to control when analyzed quantitatively. Conclusions 5% sodium hypochlorite showed maximum antibacterial activity against E. Faecalis biofilm formed on tooth substrate. Triphala, green tea polyphenols and MTAD showed statistically significant antibacterial activity. The use of herbal alternatives as a root canal irrigant might prove to be advantageous considering the several undesirable characteristics of NaOCl.
Hari Mohan - One of the best experts on this subject based on the ideXlab platform.
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free radical scavenging reactions and phytochemical analysis of Triphala an ayurvedic formulation
Current Science, 2006Co-Authors: G H Naik, K I Priyadarsini, Hari MohanAbstract:In order to understand the factors responsible for the potent antioxidant and radioprotecting ability of Triphala, it has been evaluated for radical scavenging ability, xanthine oxidase inhibitory activity and phytochemical (phenolics) content. The radical scavenging experiments were carried out using fast reaction kinetic tools and the reactivity of Triphala towards different radicals such as hydroxyl radicals, superoxide radicals, DPPH and ABTS • •was determined. When Triphala was tested for superoxide radical scavenging activity using xanthine and xanthine oxidase assay, it was observed that in addition to reacting with superoxide radical, it also inhibited uric acid formation, indicative of xanthine oxidase enzyme inhibitory activity. Phytochemical ana lysis showed that Triphala is rich in phenols/polyphenols (38 ± 3%) and tannins (35 ± 3%), while flavonoids were found to be absent. HPLC analysis showed that Triphala contains 73 ± 5 mg gallic acid per gram of Triphala, which was found to increase to 150 ± 5 mg/g upon acid hydrolysis. Relevance of these studies to the antioxidant and radio protection properties of Triphala has also been discussed.
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in vitro antioxidant studies and free radical reactions of Triphala an ayurvedic formulation and its constituents
Phytotherapy Research, 2005Co-Authors: G H Naik, Kaushala Prasad Mishra, K I Priyadarsini, R G Bhagirathi, B Mishra, M M Banavalikar, Hari MohanAbstract:The aqueous extract of the fruits of Emblica officinalis (T1), Terminalia chebula (T2) and Terminalia belerica (T3) and their equiproportional mixture Triphala were evaluated for their in vitro antioxidant activity. gamma-Radiation induced strand break formation in plasmid DNA (pBR322) was effectively inhibited by Triphala and its constituents in the concentration range 25-200 microg/mL with a percentage inhibition of T1 (30%-83%), T2 (21%-71%), T3 (8%-58%) and Triphala (17%-63%). They also inhibited radiation induced lipid peroxidation in rat liver microsomes effectively with IC(50) values less than 15 microg/mL. The extracts were found to possess the ability to scavenge free radicals such as DPPH and superoxide. As the phenolic compounds present in these extracts are mostly responsible for their radical scavenging activity, the total phenolic contents present in these extracts were determined and expressed in terms of gallic acid equivalents and were found to vary from 33% to 44%. These studies revealed that all three constituents of Triphala are active and they exhibit slightly different activities under different conditions. T1 shows greater efficiency in lipid peroxidation and plasmid DNA assay, while T2 has greater radical scavenging activity. Thus their mixture, Triphala, is expected to be more efficient due to the combined activity of the individual components.
Evanprince Sabina - One of the best experts on this subject based on the ideXlab platform.
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antiinflammatory effect of the indian ayurvedic herbal formulation Triphala on adjuvant induced arthritis in mice
Phytotherapy Research, 2007Co-Authors: Mahaboobkhan Rasool, Evanprince SabinaAbstract:In the present study, attempts have been made to evaluate the antiarthritic effect of the Indian Ayurvedic herbal formulation Triphala on adjuvant-induced arthritis in mice and to compare it with that of the non-steroidal antiinflammatory drug indomethacin. Arthritis was induced by intradermal injection of complete Freund's adjuvant (0.1 mL) into the right hind paw of Swiss albino mice. Triphala (1 g/kg/bxwt) and indomethacin (3 mg/kg/bxwt) were administered orally for 8 days (from day 11 to 18) after adjuvant injection. The levels of lysosomal enzymes, tissue marker enzymes, glycoproteins and paw thickness were increased in adjuvant-induced arthritic animals. The body weight was found to be reduced when compared with the control animals. These physical and biochemical changes observed in arthritic animals were altered significantly to near normal conditions after oral administration of Triphala (1 g/kg/bxwt). The results obtained clearly indicate the fact that the Indian Ayurvedic herbal formulation Triphala has promising antiinflammatory activity.
Dayanandan Mani - One of the best experts on this subject based on the ideXlab platform.
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Protective effect of Triphala Rasayana against paracetamol-induced hepato–renal toxicity in mice
Elsevier, 2015Co-Authors: Dewasya Pratap Singh, Dayanandan ManiAbstract:Background: Paracetamol, a widely used analgesic and antipyretic, is known to cause liver and renal injury in humans when administered in higher and repeated doses that cause acute liver injury. Triphala is a well-known Ayurvedic Rasayana formulation that is prescribed for balancing of Vata, Pitta and Kapha. Traditionally, it is used for the treatment of liver and kidney diseases. Objective: The present study was undertaken to examine the protective effect of Triphala extract against paracetamol-induced hepato–renal injury in Swiss albino mice. Materials and Methods: Swiss albino mice (weight 20–25 g) were used in this study. The mice were divided into five groups of six animals each. The aqueous extract of Triphala was given orally at two different doses (100 and 300 mg/kg body weight) for seven consecutive days, followed by a single intraperitoneal injection of paracetamol (500 mg/kg body weight) to induce hepato–renal toxicity. Serum levels of liver enzymes, aspartate aminotransferase (AST), alanine aminotransferase (ALT), alkaline phosphatase (ALP), bilirubin, creatinine, urea and uric acid were measured as indices of liver and renal injury. Statistics: All the statistical analyses were performed with the help of one-way analysis of variance (ANOVA) followed by Student–Newman–Keuls test as post hoc test. Results were considered statistically significant when P < 0.05. Results: Pre-treatment with Triphala extract at 100 mg/kg and 300 mg/kg body weight exhibited a significant (P < 0.01) hepatoprotective activity. The protective effect of Triphala extract at 300 mg/kg body weight appears more effective than 100 mg/kg body weight. Conclusion: The present study gives an evidence of the protective role of Triphala extract against paracetamol-induced hepato–renal toxicity and validates its traditional claim in the Ayurveda system
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protective effect of Triphala rasayana against paracetamol induced hepato renal toxicity in mice
Journal of Ayurveda and Integrative Medicine, 2014Co-Authors: Dewasya Pratap Singh, Dayanandan ManiAbstract:Background: Paracetamol, a widely used analgesic and antipyretic, is known to cause liver and renal injury in humans when administered in higher and repeated doses that cause acute liver injury. Triphala is a well-known Ayurvedic Rasayana formulation that is prescribed for balancing of Vata, Pitta and Kapha. Traditionally, it is used for the treatment of liver and kidney diseases. Objective: The present study was undertaken to examine the protective effect of Triphala extract against paracetamol-induced hepato–renal injury in Swiss albino mice. Materials and Methods: Swiss albino mice (weight 20–25 g) were used in this study. The mice were divided into five groups of six animals each. The aqueous extract of Triphala was given orally at two different doses (100 and 300 mg/kg body weight) for seven consecutive days, followed by a single intraperitoneal injection of paracetamol (500 mg/kg body weight) to induce hepato–renal toxicity. Serum levels of liver enzymes, aspartate aminotransferase (AST), alanine aminotransferase (ALT), alkaline phosphatase (ALP), bilirubin, creatinine, urea and uric acid were measured as indices of liver and renal injury. Statistics: All the statistical analyses were performed with the help of one-way analysis of variance (ANOVA) followed by Student–Newman–Keuls test as post hoc test. Results were considered statistically significant when P Results: Pre-treatment with Triphala extract at 100 mg/kg and 300 mg/kg body weight exhibited a significant ( P Triphala extract at 300 mg/kg body weight appears more effective than 100 mg/kg body weight. Conclusion: The present study gives an evidence of the protective role of Triphala extract against paracetamol-induced hepato–renal toxicity and validates its traditional claim in the Ayurveda system.
J Prabhakar - One of the best experts on this subject based on the ideXlab platform.
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Evaluation of antimicrobial efficacy of Triphala (an Indian Ayurvedic herbal formulation) and 0.2% chlorhexidine against Streptococcus mutans biofilm formed on tooth substrate: An in vitro study
Wolters Kluwer Medknow Publications, 2014Co-Authors: J Prabhakar, M S Priya, S Balagopal, S Selvi, M SenthilkumarAbstract:Background: Streptococcus mutans is one of the most important cariogenic species of the human oral microbial flora. Biofilm style of microbial growth thought to resist the actions of antimicrobials. Aim: The purpose of this study was to evaluate the antimicrobial efficacy of Triphala, and 0.2% chlorhexidine against S. mutans biofilm formed on tooth substrate. Settings and Design: Randomized control trial. Methods: Extracted human mandibular premolars sectioned below the cemento-enamel junction were placed in the tissue culture wells exposing the crown surface to S. mutans to form a biofilm. At the end of 3 rd and 7 th day, all groups were treated for 10 min with the test solutions and control and were analyzed qualitatively and quantitatively. Statistical Analysis Used: One-way ANOVA. Results: Qualitative assay with 3 days biofilm showed complete inhibition of bacterial growth with Triphala, but 0.2% chlorhexidine and saline showed the presence of bacterial growth. In quantitative analysis, 0.2% chlorhexidine and Saline treated tooth samples have shown 1052 Χ 10 4 ± 15.1 Χ 10 4 CFU/ml, 141.3 Χ 10 9 ± 2.1Χ10 9 CFU/ml, respectively. Qualitative assay with 7 days biofilm on crown portion showed dense growth when treated with 0.2% chlorhexidine and saline, whereas Triphala has shown minimal growth. In Quantitative analysis, Triphala showed statistically significant result when compared with 0.2% chlorhexidine and saline. Conclusion: Triphala showed statistically significant antibacterial activity against S. mutans biofilm formed on tooth substrate. The incorporation of Triphala in mouth rinse could prove to be effective in reducing S. mutans count in the oral cavity
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evaluation of antimicrobial efficacy of herbal alternatives Triphala and green tea polyphenols mtad and 5 sodium hypochlorite against enterococcus faecalis biofilm formed on tooth substrate an in vitro study
Journal of Endodontics, 2010Co-Authors: J Prabhakar, M Senthilkumar, M S Priya, K Mahalakshmi, P K Sehgal, V G SukumaranAbstract:Abstract Introduction The purpose of this study was to evaluate the antimicrobial efficacy of Triphala, green tea polyphenols (GTP), MTAD, and 5% sodium hypochlorite against E. faecalis biofilm formed on tooth substrate. Methods Extracted human teeth were biomechanically prepared, vertically sectioned, placed in the tissue culture wells exposing the root canal surface to E. faecalis to form a biofilm. At the end of the 3rd and 6th weeks all groups were treated for 10 minutes with the test solutions and control and were analyzed qualitatively and quantitatively. Results Qualitative assay with 3-week biofilm showed complete inhibition of bacterial growth with Triphala, MTAD and NaOCl, except GTP and saline, which showed presence of bacterial growth. In quantitative analysis, GTP- and saline-treated tooth samples have shown 1516 ± 17.2 CFU/mL, 156.4 × 10 9 ± 3.1 × 10 9 CFU/mL respectively. Qualitative assay with 6-week biofilm showed growth when treated with Triphala, GTP and MTAD whereas NaOCl has shown complete inhibition. All groups except NaOCl showed eight log reduction when compared to control when analyzed quantitatively. Conclusions 5% sodium hypochlorite showed maximum antibacterial activity against E. Faecalis biofilm formed on tooth substrate. Triphala, green tea polyphenols and MTAD showed statistically significant antibacterial activity. The use of herbal alternatives as a root canal irrigant might prove to be advantageous considering the several undesirable characteristics of NaOCl.
