The Experts below are selected from a list of 231 Experts worldwide ranked by ideXlab platform
Ilhan Gurbuz - One of the best experts on this subject based on the ideXlab platform.
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Evaluation of the antiulcerogenic activity profile of a flavonol diglucoside from Equisetum palustre L.
Journal of ethnopharmacology, 2010Co-Authors: Erdem Yesilada, Ilhan GurbuzAbstract:Abstract Ethnopharmacological relevance The aerial parts of Equisetum palustre L. are used to treat peptic ulcer disease in Turkey. In a previous study, a flavonol diglucoside i.e., kaempferol 3- O -1″-β- d -glucopyranosyl-3- O -1″′-β- d -glucopyranoside (KGG) was isolated as the major antiulcerogenic constituent from the plant. Aim of the study The present study was undertaken to evaluate the antiulcer activity profile of KGG using various in vivo experimental ulcer models as well as by assessing gastric biochemical parameters. Material and methods KGG was obtained from the ethanol extract of the aerial parts of the plant by successive chromatographical methods. The activity profile of the compound was investigated using several Ulcerogenesis models such as indomethacin-, indomethacin plus HCl/EtOH-, cysteamine-, serotonin-, N G -nitro- l -arginine methyl ester plus EtOH-, diethyldithiocarbamate-, N -ethylmaleimide plus EtOH-, water immersion and restraint stress-, pyloric ligation-induced ulcers. In addition, effects of KGG on the biochemical parameters of gastric juice; i.e., inhibition of titratable gastric acidity, acid output, gastric pH, gastric secretion volume and peptic activity were studied. Results KGG exerted statistically significant gastroprotective activity against indomethacin-, indomethacin plus HCl/EtOH- and N -ethylmaleimide plus EtOH-induced Ulcerogenesis. Moreover, KGG demonstrated weak activity against N G -nitro- l -arginine methyl ester plus EtOH, water immersion and immobilization-induced stress, pyloric ligation-induced and diethyldithiocarbamate-induced gastric ulcer models, and also it was ineffective in the prevention of ulcers induced by serotonin and cysteamine. On the other hand, among the gastric biochemical parameters studied, KGG was only found to increase the gastric acid pH from 2.03 to 3.35. Conclusion Results of this investigation have clearly demonstrated that KGG was found to improve the cytoprotective mechanisms of the gastric mucosa. On the other hand, a weak activity profile was observed on the parameters affecting the gastric acidity (water immersion and restraint-induced-, pyloric ligation-induced-Ulcerogenesis and titratable acidity).
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isolation of anti ulcerogenic sesquiterpene lactones from centaurea solstitialis l ssp solstitialis through bioassay guided fractionation procedures in rats
Journal of Ethnopharmacology, 2004Co-Authors: Erdem Yesilada, Ilhan Gurbuz, Irem I Tatli, Erdal Bedir, Ikhlas A. KhanAbstract:Abstract The fresh spiny flowers of Centaurea solstitialis ssp. solstitialis (CSS) are used for the treatment of peptic ulcers in Turkey. Ethanol (80%) extract of CSS exhibited significant anti-ulcerogenic effect on the ethanol-induced Ulcerogenesis model in rats. The ethanol extract was further fractionated by successive solvent extractions with n-hexane, chloroform, ethyl acetate and n-butanol. All fractions showed significant anti-ulcerogenic activity, however, the effect of the chloroform fraction was found to be more prominent with 99.5% ulcer inhibition. Bioassay-guided fractionation yielded sesquiterpene lactones as the active components. The main components responsible for the activity of the chloroform fraction were determined as chlorojanerin and 13-acetyl solstitialin A which were elucidated by HR-ESI and 1H, 13C and 2D NMR spectroscopic techniques.
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Evaluation of in vivo Biological Activity Profile of Isoorientin
Zeitschrift fur Naturforschung. C Journal of biosciences, 2004Co-Authors: Esra Küpeli, Ilhan Gurbuz, Mustafa Aslan, Erdem YesiladaAbstract:Anti-nociceptive, anti-inflammatory and gastroprotective activities of the known C-glycosyl flavonoid, isoorientin, were studied in rats and mice. For the anti-nociceptive activity assessment the p-benzoquinone-induced writing test, for the anti-inflammatory activity the carrageenan-induced hind paw edema model in mice, and for the gastroprotective activity the EtOH-induced Ulcerogenesis model in rats were used. Isoorientin was shown to possess significant anti-nociceptive and anti-inflammatory activities at 15 mg/kg and 30 mg/kg doses, without inducing any apparent acute toxicity as well as gastric damage. However, the compound did not possess any significant gastroprotective activity against EtOH-induced Ulcerogenesis.
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Anti-ulcerogenic lignans from Taxus baccata L.
Zeitschrift fur Naturforschung. C Journal of biosciences, 2004Co-Authors: Ilhan Gurbuz, Erdem Yesilada, Nurgun Erdemoglu, Bilge SenerAbstract:Four lignan type compounds, lariciresinol, taxiresinol, isolariciresinol and 3-demethyl-iso-lariciresinol, were isolated from the heartwood of Taxus baccata L. (Taxaceae) growing in Turkey through chromatographic techniques. In vivo anti-ulcerogenic potency of these compounds was investigated on ethanol-induced Ulcerogenesis model in rats at two different doses, 50 and 100 mg/kg. All compounds were shown to possess significant anti-ulcerogenic activity at both doses. However, the effect of taxiresinol was the most prominent.
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ANTI-ULCEROGENIC ACTIVITY OF FERULAGO ISAURICA AND F. SYRIACA GROWING IN TURKEY
Galenos Yayinevi, 2004Co-Authors: Ilhan Gurbuz, Ceyda S. Erdurak, Maksut Çoskun, Erdem YesiladaAbstract:Abstract In order to evaluate the claimed folkloric utilisation of Ferulago specses, the anti-ulcerogenic activities of lyophilised aqueous extracts obtained from the aerial parts and roots of Ferulago isaurica Pesmen and F. syriaca Boiss. (Apiaceae) have been studied using etOH-induced Ulcerogenesis model in rats. All plant parts exhibited statistically significant gastroprotective activity against in vivo model eöployed in the present study however the highest activity was observed for the roots of F. isaurica (100%). Özet Ferulago türlerinin halk arasindaki kullanimini degerlendirmek üzere, Ferulago isaurica Pesmen ve F. syriaca Boiss.`in (Apiaceae) toprak üstü kisimlari ve köklerinden ayri ayri hazirlananliyofilize sulu ekstrelerin antiülserojenik etkisi siçanlarda EtOH ile olusturulan ülser modeli ile çalisilmistir. Tüm ekstreler uygulanan modele karsi istatistiksel olarak belirgin bir gastroptektif aktivite gösterirken, en yüksek aktivite F. isaurica köklerinde görülmüstür (%100)
Erdem Yesilada - One of the best experts on this subject based on the ideXlab platform.
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Evaluation of the antiulcerogenic activity profile of a flavonol diglucoside from Equisetum palustre L.
Journal of ethnopharmacology, 2010Co-Authors: Erdem Yesilada, Ilhan GurbuzAbstract:Abstract Ethnopharmacological relevance The aerial parts of Equisetum palustre L. are used to treat peptic ulcer disease in Turkey. In a previous study, a flavonol diglucoside i.e., kaempferol 3- O -1″-β- d -glucopyranosyl-3- O -1″′-β- d -glucopyranoside (KGG) was isolated as the major antiulcerogenic constituent from the plant. Aim of the study The present study was undertaken to evaluate the antiulcer activity profile of KGG using various in vivo experimental ulcer models as well as by assessing gastric biochemical parameters. Material and methods KGG was obtained from the ethanol extract of the aerial parts of the plant by successive chromatographical methods. The activity profile of the compound was investigated using several Ulcerogenesis models such as indomethacin-, indomethacin plus HCl/EtOH-, cysteamine-, serotonin-, N G -nitro- l -arginine methyl ester plus EtOH-, diethyldithiocarbamate-, N -ethylmaleimide plus EtOH-, water immersion and restraint stress-, pyloric ligation-induced ulcers. In addition, effects of KGG on the biochemical parameters of gastric juice; i.e., inhibition of titratable gastric acidity, acid output, gastric pH, gastric secretion volume and peptic activity were studied. Results KGG exerted statistically significant gastroprotective activity against indomethacin-, indomethacin plus HCl/EtOH- and N -ethylmaleimide plus EtOH-induced Ulcerogenesis. Moreover, KGG demonstrated weak activity against N G -nitro- l -arginine methyl ester plus EtOH, water immersion and immobilization-induced stress, pyloric ligation-induced and diethyldithiocarbamate-induced gastric ulcer models, and also it was ineffective in the prevention of ulcers induced by serotonin and cysteamine. On the other hand, among the gastric biochemical parameters studied, KGG was only found to increase the gastric acid pH from 2.03 to 3.35. Conclusion Results of this investigation have clearly demonstrated that KGG was found to improve the cytoprotective mechanisms of the gastric mucosa. On the other hand, a weak activity profile was observed on the parameters affecting the gastric acidity (water immersion and restraint-induced-, pyloric ligation-induced-Ulcerogenesis and titratable acidity).
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isolation of anti ulcerogenic sesquiterpene lactones from centaurea solstitialis l ssp solstitialis through bioassay guided fractionation procedures in rats
Journal of Ethnopharmacology, 2004Co-Authors: Erdem Yesilada, Ilhan Gurbuz, Irem I Tatli, Erdal Bedir, Ikhlas A. KhanAbstract:Abstract The fresh spiny flowers of Centaurea solstitialis ssp. solstitialis (CSS) are used for the treatment of peptic ulcers in Turkey. Ethanol (80%) extract of CSS exhibited significant anti-ulcerogenic effect on the ethanol-induced Ulcerogenesis model in rats. The ethanol extract was further fractionated by successive solvent extractions with n-hexane, chloroform, ethyl acetate and n-butanol. All fractions showed significant anti-ulcerogenic activity, however, the effect of the chloroform fraction was found to be more prominent with 99.5% ulcer inhibition. Bioassay-guided fractionation yielded sesquiterpene lactones as the active components. The main components responsible for the activity of the chloroform fraction were determined as chlorojanerin and 13-acetyl solstitialin A which were elucidated by HR-ESI and 1H, 13C and 2D NMR spectroscopic techniques.
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Evaluation of in vivo Biological Activity Profile of Isoorientin
Zeitschrift fur Naturforschung. C Journal of biosciences, 2004Co-Authors: Esra Küpeli, Ilhan Gurbuz, Mustafa Aslan, Erdem YesiladaAbstract:Anti-nociceptive, anti-inflammatory and gastroprotective activities of the known C-glycosyl flavonoid, isoorientin, were studied in rats and mice. For the anti-nociceptive activity assessment the p-benzoquinone-induced writing test, for the anti-inflammatory activity the carrageenan-induced hind paw edema model in mice, and for the gastroprotective activity the EtOH-induced Ulcerogenesis model in rats were used. Isoorientin was shown to possess significant anti-nociceptive and anti-inflammatory activities at 15 mg/kg and 30 mg/kg doses, without inducing any apparent acute toxicity as well as gastric damage. However, the compound did not possess any significant gastroprotective activity against EtOH-induced Ulcerogenesis.
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Anti-ulcerogenic lignans from Taxus baccata L.
Zeitschrift fur Naturforschung. C Journal of biosciences, 2004Co-Authors: Ilhan Gurbuz, Erdem Yesilada, Nurgun Erdemoglu, Bilge SenerAbstract:Four lignan type compounds, lariciresinol, taxiresinol, isolariciresinol and 3-demethyl-iso-lariciresinol, were isolated from the heartwood of Taxus baccata L. (Taxaceae) growing in Turkey through chromatographic techniques. In vivo anti-ulcerogenic potency of these compounds was investigated on ethanol-induced Ulcerogenesis model in rats at two different doses, 50 and 100 mg/kg. All compounds were shown to possess significant anti-ulcerogenic activity at both doses. However, the effect of taxiresinol was the most prominent.
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ANTI-ULCEROGENIC ACTIVITY OF FERULAGO ISAURICA AND F. SYRIACA GROWING IN TURKEY
Galenos Yayinevi, 2004Co-Authors: Ilhan Gurbuz, Ceyda S. Erdurak, Maksut Çoskun, Erdem YesiladaAbstract:Abstract In order to evaluate the claimed folkloric utilisation of Ferulago specses, the anti-ulcerogenic activities of lyophilised aqueous extracts obtained from the aerial parts and roots of Ferulago isaurica Pesmen and F. syriaca Boiss. (Apiaceae) have been studied using etOH-induced Ulcerogenesis model in rats. All plant parts exhibited statistically significant gastroprotective activity against in vivo model eöployed in the present study however the highest activity was observed for the roots of F. isaurica (100%). Özet Ferulago türlerinin halk arasindaki kullanimini degerlendirmek üzere, Ferulago isaurica Pesmen ve F. syriaca Boiss.`in (Apiaceae) toprak üstü kisimlari ve köklerinden ayri ayri hazirlananliyofilize sulu ekstrelerin antiülserojenik etkisi siçanlarda EtOH ile olusturulan ülser modeli ile çalisilmistir. Tüm ekstreler uygulanan modele karsi istatistiksel olarak belirgin bir gastroptektif aktivite gösterirken, en yüksek aktivite F. isaurica köklerinde görülmüstür (%100)
Kunio Nakashima - One of the best experts on this subject based on the ideXlab platform.
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Prolactin prevents acute stress-induced hypocalcemia and Ulcerogenesis by acting in the brain of rat.
Endocrinology, 2004Co-Authors: Takahiko Fujikawa, Hideaki Soya, Kellie L. K. Tamashiro, Randall R. Sakai, Bruce S. Mcewen, Naoya Nakai, Masato Ogata, Ikukatsu Suzuki, Kunio NakashimaAbstract:Stress causes hypocalcemia and Ulcerogenesis in rats. In rats under stressful conditions, a rapid and transient increase in circulating prolactin (PRL) is observed, and this enhanced PRL induces PRL receptors (PRLR) in the choroid plexus of rat brain. In this study we used restraint stress in water to elucidate the mechanism by which PRLR in the rat brain mediate the protective effect of PRL against stress-induced hypocalcemia and Ulcerogenesis. We show that rat PRL acts through the long form of PRLR in the hypothalamus. This is followed by an increase in the long form of PRLR mRNA expression in the choroid plexus of the brain, which provides protection against restraint stress in water-induced hypocalcemia and gastric erosions. We also show that PRL induces the expression of PRLR protein and corticotropin-releasing factor mRNA in the paraventricular nucleus. These results suggest that the PRL levels increase in response to stress, and it moves from the circulation to the cerebrospinal fluid to act on the central nervous system and thereby plays an important role in helping to protect against acute stress-induced hypocalcemia and gastric erosions.
Takahiko Fujikawa - One of the best experts on this subject based on the ideXlab platform.
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0013-7227/04/$15.00/0 Endocrinology 145(4):2006–2013 Printed in U.S.A. Copyright © 2004 by The Endocrine Society doi: 10.1210/en.2003-1446 Prolactin Prevents Acute Stress-Induced Hypocalcemia and Ulcerogenesis by Acting in the Brain of Rat
2013Co-Authors: Takahiko Fujikawa, Hideaki Soya, Kellie L. K. Tamashiro, Randall R. Sakai, Bruce S. Mcewen, Naoya Nakai, Masato Ogata, Ikukatsu SuzukiAbstract:Stress causes hypocalcemia and Ulcerogenesis in rats. In rats under stressful conditions, a rapid and transient increase in circulating prolactin (PRL) is observed, and this enhanced PRL induces PRL receptors (PRLR) in the choroid plexus of rat brain. In this study we used restraint stress in water to elucidate the mechanism by which PRLR in the rat brain mediate the protective effect of PRL against stress-induced hypocalcemia and Ulcerogenesis. We show that rat PRL acts through the long form of PRLR in the hypothalamus. This is followed by an increase in the long form of PRLR mRNA expression in the choroid plexus of the brain, which provides protection against restraint stress in water-induced hypocalcemia and gastric erosions. We also show that PRL induces the expression of PRLR protein and corticotropin-releasing factor mRNA in the paraventricular nucleus. These results sugges
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Prolactin prevents acute stress-induced hypocalcemia and Ulcerogenesis by acting in the brain of rat.
Endocrinology, 2004Co-Authors: Takahiko Fujikawa, Hideaki Soya, Kellie L. K. Tamashiro, Randall R. Sakai, Bruce S. Mcewen, Naoya Nakai, Masato Ogata, Ikukatsu Suzuki, Kunio NakashimaAbstract:Stress causes hypocalcemia and Ulcerogenesis in rats. In rats under stressful conditions, a rapid and transient increase in circulating prolactin (PRL) is observed, and this enhanced PRL induces PRL receptors (PRLR) in the choroid plexus of rat brain. In this study we used restraint stress in water to elucidate the mechanism by which PRLR in the rat brain mediate the protective effect of PRL against stress-induced hypocalcemia and Ulcerogenesis. We show that rat PRL acts through the long form of PRLR in the hypothalamus. This is followed by an increase in the long form of PRLR mRNA expression in the choroid plexus of the brain, which provides protection against restraint stress in water-induced hypocalcemia and gastric erosions. We also show that PRL induces the expression of PRLR protein and corticotropin-releasing factor mRNA in the paraventricular nucleus. These results suggest that the PRL levels increase in response to stress, and it moves from the circulation to the cerebrospinal fluid to act on the central nervous system and thereby plays an important role in helping to protect against acute stress-induced hypocalcemia and gastric erosions.
Ikukatsu Suzuki - One of the best experts on this subject based on the ideXlab platform.
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0013-7227/04/$15.00/0 Endocrinology 145(4):2006–2013 Printed in U.S.A. Copyright © 2004 by The Endocrine Society doi: 10.1210/en.2003-1446 Prolactin Prevents Acute Stress-Induced Hypocalcemia and Ulcerogenesis by Acting in the Brain of Rat
2013Co-Authors: Takahiko Fujikawa, Hideaki Soya, Kellie L. K. Tamashiro, Randall R. Sakai, Bruce S. Mcewen, Naoya Nakai, Masato Ogata, Ikukatsu SuzukiAbstract:Stress causes hypocalcemia and Ulcerogenesis in rats. In rats under stressful conditions, a rapid and transient increase in circulating prolactin (PRL) is observed, and this enhanced PRL induces PRL receptors (PRLR) in the choroid plexus of rat brain. In this study we used restraint stress in water to elucidate the mechanism by which PRLR in the rat brain mediate the protective effect of PRL against stress-induced hypocalcemia and Ulcerogenesis. We show that rat PRL acts through the long form of PRLR in the hypothalamus. This is followed by an increase in the long form of PRLR mRNA expression in the choroid plexus of the brain, which provides protection against restraint stress in water-induced hypocalcemia and gastric erosions. We also show that PRL induces the expression of PRLR protein and corticotropin-releasing factor mRNA in the paraventricular nucleus. These results sugges
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Prolactin prevents acute stress-induced hypocalcemia and Ulcerogenesis by acting in the brain of rat.
Endocrinology, 2004Co-Authors: Takahiko Fujikawa, Hideaki Soya, Kellie L. K. Tamashiro, Randall R. Sakai, Bruce S. Mcewen, Naoya Nakai, Masato Ogata, Ikukatsu Suzuki, Kunio NakashimaAbstract:Stress causes hypocalcemia and Ulcerogenesis in rats. In rats under stressful conditions, a rapid and transient increase in circulating prolactin (PRL) is observed, and this enhanced PRL induces PRL receptors (PRLR) in the choroid plexus of rat brain. In this study we used restraint stress in water to elucidate the mechanism by which PRLR in the rat brain mediate the protective effect of PRL against stress-induced hypocalcemia and Ulcerogenesis. We show that rat PRL acts through the long form of PRLR in the hypothalamus. This is followed by an increase in the long form of PRLR mRNA expression in the choroid plexus of the brain, which provides protection against restraint stress in water-induced hypocalcemia and gastric erosions. We also show that PRL induces the expression of PRLR protein and corticotropin-releasing factor mRNA in the paraventricular nucleus. These results suggest that the PRL levels increase in response to stress, and it moves from the circulation to the cerebrospinal fluid to act on the central nervous system and thereby plays an important role in helping to protect against acute stress-induced hypocalcemia and gastric erosions.