The Experts below are selected from a list of 3720 Experts worldwide ranked by ideXlab platform
Edward J. Kennelly - One of the best experts on this subject based on the ideXlab platform.
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lc ms metabolomics and chemotaxonomy of caffeine containing holly ilex species and related taxa in the aquifoliaceae
Journal of Agricultural and Food Chemistry, 2019Co-Authors: Adam Negrin, Chunlin Long, Timothy J. Motley, Edward J. KennellyAbstract:Ilex species have been consumed traditionally as medicinal teas worldwide. Though the presence of caffeine has been reported in several species, little is known about secondary-metabolite diversity within and among these taxa. Leaf samples of Ilex guayusa, Ilex paraguariensis, and Ilex vomitoria were analyzed by liquid chromatography-mass spectrometry and comparative metabolite profiling with Ilex cassine and other Ilex species to identify chemotaxonomic markers, delimit species, and provide an assessment of chemodiversity. Purine alkaloids were detected and quantified in I. guayusa, I. paraguariensis, and I. vomitoria. Reports of caffeine for I. cassine were not corroborated, suggesting that I. vomitoria was the traditional source of the Native North American tea yaupon. The tetramethyluric acid, theacrine, was detected for the first time in the genus Ilex as a low-level chemotaxonomic marker in I. vomitoria samples. Chemotaxonomy and metabolomics support a close relationship for caffeine-containing Ilex species.
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LC-MS Metabolomics and Chemotaxonomy of Caffeine-Containing Holly (Ilex) Species and Related Taxa in the Aquifoliaceae
2019Co-Authors: Adam Negrin, Chunlin Long, Timothy J. Motley, Edward J. KennellyAbstract:Ilex species have been consumed traditionally as medicinal teas worldwide. Though the presence of caffeine has been reported in several species, little is known about secondary-metabolite diversity within and among these taxa. Leaf samples of Ilex guayusa, Ilex paraguariensis, and Ilex vomitoria were analyzed by liquid chromatography–mass spectrometry and comparative metabolite profiling with Ilex cassine and other Ilex species to identify chemotaxonomic markers, delimit species, and provide an assessment of chemodiversity. Purine alkaloids were detected and quantified in I. guayusa, I. paraguariensis, and I. vomitoria. Reports of caffeine for I. cassine were not corroborated, suggesting that I. vomitoria was the traditional source of the Native North American tea yaupon. The tetramethyluric acid, theacrine, was detected for the first time in the genus Ilex as a low-level chemotaxonomic marker in I. vomitoria samples. Chemotaxonomy and metabolomics support a close relationship for caffeine-containing Ilex species
Nuria Busquets - One of the best experts on this subject based on the ideXlab platform.
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CUlex flavivirus infection in a CUlex pipiens mosquito colony and its effects on vector competence for Rift Valley fever phlebovirus
Parasites & Vectors, 2018Co-Authors: Sandra Talavera, Lotty Birnberg, Ana I. Núñez, Francesc Muñoz-muñoz, Ana Vazquez, Nuria BusquetsAbstract:Rift Valley fever is a mosquito-borne zoonotic disease that affects domestic ruminants and humans. CUlex flavivirus is an insect-specific flavivirus that naturally exists in field mosquito populations. The influence of CUlex flavivirus on Rift Valley fever phlebovirus (RVFV) vector competence of CUlex pipiens has not been investigated. CUlex flavivirus infection in a Cx. pipiens colony was studied by CUlex flavivirus oral feeding and intrathoracical inoculation. Similarly, vector competence of Cx. pipiens infected with CUlex flavivirus was evaluated for RVFV. Infection, dissemination, transmission rates and transmission efficiency of CUlex flavivirus-infected and non-infected Cx. pipiens artificially fed with RVFV infected blood were assessed. CUlex flavivirus was able to infect Cx. pipiens after intrathoracically inoculation in Cx. pipiens mosquitos but not after CUlex flavivirus oral feeding. CUlex flavivirus did not affect RVFV infection, dissemination and transmission in Cx. pipiens mosquitoes. RVFV could be detected from saliva of both the CUlex flavivirus-positive and negative Cx. pipiens females without significant differences. Moreover, RVFV did not interfere with the CUlex flavivirus infection in Cx. pipiens mosquitoes. CUlex flavivirus infected and non-infected Cx. pipiens transmit RVFV. CUlex flavivirus existing in field-collected Cx. pipiens populations does not affect their vector competence for RVFV. CUlex flavivirus may not be an efficient tool for RVFV control in mosquitoes.
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CUlex flavivirus infection in a CUlex pipiens mosquito colony and its effects on vector competence for Rift Valley fever phlebovirus
BMC, 2018Co-Authors: Sandra Talavera, Lotty Birnberg, Ana I. Núñez, Francesc Muñoz-muñoz, Ana Vazquez, Nuria BusquetsAbstract:Abstract Background Rift Valley fever is a mosquito-borne zoonotic disease that affects domestic ruminants and humans. CUlex flavivirus is an insect-specific flavivirus that naturally exists in field mosquito populations. The influence of CUlex flavivirus on Rift Valley fever phlebovirus (RVFV) vector competence of CUlex pipiens has not been investigated. Methods CUlex flavivirus infection in a Cx. pipiens colony was studied by CUlex flavivirus oral feeding and intrathoracical inoculation. Similarly, vector competence of Cx. pipiens infected with CUlex flavivirus was evaluated for RVFV. Infection, dissemination, transmission rates and transmission efficiency of CUlex flavivirus-infected and non-infected Cx. pipiens artificially fed with RVFV infected blood were assessed. Results CUlex flavivirus was able to infect Cx. pipiens after intrathoracically inoculation in Cx. pipiens mosquitos but not after CUlex flavivirus oral feeding. CUlex flavivirus did not affect RVFV infection, dissemination and transmission in Cx. pipiens mosquitoes. RVFV could be detected from saliva of both the CUlex flavivirus-positive and negative Cx. pipiens females without significant differences. Moreover, RVFV did not interfere with the CUlex flavivirus infection in Cx. pipiens mosquitoes. Conclusions CUlex flavivirus infected and non-infected Cx. pipiens transmit RVFV. CUlex flavivirus existing in field-collected Cx. pipiens populations does not affect their vector competence for RVFV. CUlex flavivirus may not be an efficient tool for RVFV control in mosquitoes
Adam Negrin - One of the best experts on this subject based on the ideXlab platform.
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lc ms metabolomics and chemotaxonomy of caffeine containing holly ilex species and related taxa in the aquifoliaceae
Journal of Agricultural and Food Chemistry, 2019Co-Authors: Adam Negrin, Chunlin Long, Timothy J. Motley, Edward J. KennellyAbstract:Ilex species have been consumed traditionally as medicinal teas worldwide. Though the presence of caffeine has been reported in several species, little is known about secondary-metabolite diversity within and among these taxa. Leaf samples of Ilex guayusa, Ilex paraguariensis, and Ilex vomitoria were analyzed by liquid chromatography-mass spectrometry and comparative metabolite profiling with Ilex cassine and other Ilex species to identify chemotaxonomic markers, delimit species, and provide an assessment of chemodiversity. Purine alkaloids were detected and quantified in I. guayusa, I. paraguariensis, and I. vomitoria. Reports of caffeine for I. cassine were not corroborated, suggesting that I. vomitoria was the traditional source of the Native North American tea yaupon. The tetramethyluric acid, theacrine, was detected for the first time in the genus Ilex as a low-level chemotaxonomic marker in I. vomitoria samples. Chemotaxonomy and metabolomics support a close relationship for caffeine-containing Ilex species.
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LC-MS Metabolomics and Chemotaxonomy of Caffeine-Containing Holly (Ilex) Species and Related Taxa in the Aquifoliaceae
2019Co-Authors: Adam Negrin, Chunlin Long, Timothy J. Motley, Edward J. KennellyAbstract:Ilex species have been consumed traditionally as medicinal teas worldwide. Though the presence of caffeine has been reported in several species, little is known about secondary-metabolite diversity within and among these taxa. Leaf samples of Ilex guayusa, Ilex paraguariensis, and Ilex vomitoria were analyzed by liquid chromatography–mass spectrometry and comparative metabolite profiling with Ilex cassine and other Ilex species to identify chemotaxonomic markers, delimit species, and provide an assessment of chemodiversity. Purine alkaloids were detected and quantified in I. guayusa, I. paraguariensis, and I. vomitoria. Reports of caffeine for I. cassine were not corroborated, suggesting that I. vomitoria was the traditional source of the Native North American tea yaupon. The tetramethyluric acid, theacrine, was detected for the first time in the genus Ilex as a low-level chemotaxonomic marker in I. vomitoria samples. Chemotaxonomy and metabolomics support a close relationship for caffeine-containing Ilex species
Sandra Talavera - One of the best experts on this subject based on the ideXlab platform.
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CUlex flavivirus infection in a CUlex pipiens mosquito colony and its effects on vector competence for Rift Valley fever phlebovirus
Parasites & Vectors, 2018Co-Authors: Sandra Talavera, Lotty Birnberg, Ana I. Núñez, Francesc Muñoz-muñoz, Ana Vazquez, Nuria BusquetsAbstract:Rift Valley fever is a mosquito-borne zoonotic disease that affects domestic ruminants and humans. CUlex flavivirus is an insect-specific flavivirus that naturally exists in field mosquito populations. The influence of CUlex flavivirus on Rift Valley fever phlebovirus (RVFV) vector competence of CUlex pipiens has not been investigated. CUlex flavivirus infection in a Cx. pipiens colony was studied by CUlex flavivirus oral feeding and intrathoracical inoculation. Similarly, vector competence of Cx. pipiens infected with CUlex flavivirus was evaluated for RVFV. Infection, dissemination, transmission rates and transmission efficiency of CUlex flavivirus-infected and non-infected Cx. pipiens artificially fed with RVFV infected blood were assessed. CUlex flavivirus was able to infect Cx. pipiens after intrathoracically inoculation in Cx. pipiens mosquitos but not after CUlex flavivirus oral feeding. CUlex flavivirus did not affect RVFV infection, dissemination and transmission in Cx. pipiens mosquitoes. RVFV could be detected from saliva of both the CUlex flavivirus-positive and negative Cx. pipiens females without significant differences. Moreover, RVFV did not interfere with the CUlex flavivirus infection in Cx. pipiens mosquitoes. CUlex flavivirus infected and non-infected Cx. pipiens transmit RVFV. CUlex flavivirus existing in field-collected Cx. pipiens populations does not affect their vector competence for RVFV. CUlex flavivirus may not be an efficient tool for RVFV control in mosquitoes.
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CUlex flavivirus infection in a CUlex pipiens mosquito colony and its effects on vector competence for Rift Valley fever phlebovirus
BMC, 2018Co-Authors: Sandra Talavera, Lotty Birnberg, Ana I. Núñez, Francesc Muñoz-muñoz, Ana Vazquez, Nuria BusquetsAbstract:Abstract Background Rift Valley fever is a mosquito-borne zoonotic disease that affects domestic ruminants and humans. CUlex flavivirus is an insect-specific flavivirus that naturally exists in field mosquito populations. The influence of CUlex flavivirus on Rift Valley fever phlebovirus (RVFV) vector competence of CUlex pipiens has not been investigated. Methods CUlex flavivirus infection in a Cx. pipiens colony was studied by CUlex flavivirus oral feeding and intrathoracical inoculation. Similarly, vector competence of Cx. pipiens infected with CUlex flavivirus was evaluated for RVFV. Infection, dissemination, transmission rates and transmission efficiency of CUlex flavivirus-infected and non-infected Cx. pipiens artificially fed with RVFV infected blood were assessed. Results CUlex flavivirus was able to infect Cx. pipiens after intrathoracically inoculation in Cx. pipiens mosquitos but not after CUlex flavivirus oral feeding. CUlex flavivirus did not affect RVFV infection, dissemination and transmission in Cx. pipiens mosquitoes. RVFV could be detected from saliva of both the CUlex flavivirus-positive and negative Cx. pipiens females without significant differences. Moreover, RVFV did not interfere with the CUlex flavivirus infection in Cx. pipiens mosquitoes. Conclusions CUlex flavivirus infected and non-infected Cx. pipiens transmit RVFV. CUlex flavivirus existing in field-collected Cx. pipiens populations does not affect their vector competence for RVFV. CUlex flavivirus may not be an efficient tool for RVFV control in mosquitoes
Valentino M Gantz - One of the best experts on this subject based on the ideXlab platform.
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optimized crispr tools and site directed transgenesis towards gene drive development in cUlex quinquefasciatus mosquitoes
Nature Communications, 2021Co-Authors: Xuechun Feng, Alena L Bishop, Victor Lopez Del Amo, Enzo Mameli, Megan Lee, Norbert Perrimon, Valentino M GantzAbstract:CUlex mosquitoes are a global vector for multiple human and animal diseases, including West Nile virus, lymphatic filariasis, and avian malaria, posing a constant threat to public health, livestock, companion animals, and endangered birds. While rising insecticide resistance has threatened the control of CUlex mosquitoes, advances in CRISPR genome-editing tools have fostered the development of alternative genetic strategies such as gene drive systems to fight disease vectors. However, though gene-drive technology has quickly progressed in other mosquitoes, advances have been lacking in CUlex. Here, we develop a CUlex-specific Cas9/gRNA expression toolkit and use site-directed homology-based transgenesis to generate and validate a CUlex quinquefasciatus Cas9-expressing line. We show that gRNA scaffold variants improve transgenesis efficiency in both CUlex quinquefasciatus and Drosophila melanogaster and boost gene-drive performance in the fruit fly. These findings support future technology development to control CUlex mosquitoes and provide valuable insight for improving these tools in other species. CUlex mosquitoes are a global vector for insect-borne diseases, though progress with genetic tools lags behind other mosquito species. Here the authors present a Cas9-based toolkit and methods that could support future gene drive development in these mosquitoes.
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optimized crispr tools and site directed transgenesis in cUlex quinquefasciatus mosquitoes for gene drive development
bioRxiv, 2021Co-Authors: Xuechun Feng, Alena L Bishop, Enzo Mameli, Megan Lee, Norbert Perrimon, Lopez V Del Amo, Valentino M GantzAbstract:CUlex mosquitoes are a global vector for multiple human and animal diseases, including West Nile virus, lymphatic filariasis, and avian malaria, posing a constant threat to public health, livestock, companion animals, and endangered birds. While rising insecticide resistance has threatened the control of CUlex mosquitoes, advances in CRISPR genome-editing tools have fostered the development of alternative genetic strategies such as gene drive systems to fight disease vectors. However, though gene-drive technology has quickly progressed in other mosquitoes, advances have been lacking in CUlex. Here, we developed a CUlex-specific Cas9/gRNA expression toolkit and used site-directed homology-based transgenesis to generate and validate a CUlex quinquefasciatus Cas9-expressing line. We showed that gRNA scaffold variants improve transgenesis efficiency in both CUlex and Drosophila and boost gene-drive performance in the fruit fly. These findings support future technology development to control CUlex mosquitoes and provide valuable insight for improving these tools in other species.
