The Experts below are selected from a list of 18 Experts worldwide ranked by ideXlab platform
Toshinao Goda - One of the best experts on this subject based on the ideXlab platform.
-
feeding rats dietary resistant starch shifts the peak of sglt1 gene expression and histone h3 acetylation on the gene from the Upper jejunum toward the Ileum
Journal of Agricultural and Food Chemistry, 2009Co-Authors: Masaya Shimada, Kazuki Mochizuki, Toshinao GodaAbstract:Sodium glucose cotransporter 1 (SGLT1) participates in the incorporation of glucose from the lumen to enterocytes in the small intestine. We examined whether dietary resistant starch (RS), an autoclaved high amylose starch that is digested more slowly than regular cornstarch in the small intestine, alters SGLT1 mRNA levels along the jejunum-Ileum of rats. The SGLT1 mRNA level was lower in the Upper jejunum in rats fed an RS diet than in those fed a regular cornstarch diet, whereas it was higher in the lower jejunum/Upper Ileum. Furthermore, using chromatin immunoprecipitation (ChIP) assay, we demonstrated that histone H3 acetylation on the promoter/enhancer and transcriptional regions was reduced in the Upper jejunum and elevated in the lower jejunum/Upper Ileum by feeding rats an RS diet. On the other hand, HNF-1 binding on the region around transcription start site of the SGLT1 gene was not altered in each jejunoileal segment by feeding rats an RS diet. Our results suggest that a shift of the expression...
Masaya Shimada - One of the best experts on this subject based on the ideXlab platform.
-
feeding rats dietary resistant starch shifts the peak of sglt1 gene expression and histone h3 acetylation on the gene from the Upper jejunum toward the Ileum
Journal of Agricultural and Food Chemistry, 2009Co-Authors: Masaya Shimada, Kazuki Mochizuki, Toshinao GodaAbstract:Sodium glucose cotransporter 1 (SGLT1) participates in the incorporation of glucose from the lumen to enterocytes in the small intestine. We examined whether dietary resistant starch (RS), an autoclaved high amylose starch that is digested more slowly than regular cornstarch in the small intestine, alters SGLT1 mRNA levels along the jejunum-Ileum of rats. The SGLT1 mRNA level was lower in the Upper jejunum in rats fed an RS diet than in those fed a regular cornstarch diet, whereas it was higher in the lower jejunum/Upper Ileum. Furthermore, using chromatin immunoprecipitation (ChIP) assay, we demonstrated that histone H3 acetylation on the promoter/enhancer and transcriptional regions was reduced in the Upper jejunum and elevated in the lower jejunum/Upper Ileum by feeding rats an RS diet. On the other hand, HNF-1 binding on the region around transcription start site of the SGLT1 gene was not altered in each jejunoileal segment by feeding rats an RS diet. Our results suggest that a shift of the expression...
Kazuki Mochizuki - One of the best experts on this subject based on the ideXlab platform.
-
feeding rats dietary resistant starch shifts the peak of sglt1 gene expression and histone h3 acetylation on the gene from the Upper jejunum toward the Ileum
Journal of Agricultural and Food Chemistry, 2009Co-Authors: Masaya Shimada, Kazuki Mochizuki, Toshinao GodaAbstract:Sodium glucose cotransporter 1 (SGLT1) participates in the incorporation of glucose from the lumen to enterocytes in the small intestine. We examined whether dietary resistant starch (RS), an autoclaved high amylose starch that is digested more slowly than regular cornstarch in the small intestine, alters SGLT1 mRNA levels along the jejunum-Ileum of rats. The SGLT1 mRNA level was lower in the Upper jejunum in rats fed an RS diet than in those fed a regular cornstarch diet, whereas it was higher in the lower jejunum/Upper Ileum. Furthermore, using chromatin immunoprecipitation (ChIP) assay, we demonstrated that histone H3 acetylation on the promoter/enhancer and transcriptional regions was reduced in the Upper jejunum and elevated in the lower jejunum/Upper Ileum by feeding rats an RS diet. On the other hand, HNF-1 binding on the region around transcription start site of the SGLT1 gene was not altered in each jejunoileal segment by feeding rats an RS diet. Our results suggest that a shift of the expression...
Isao Tada - One of the best experts on this subject based on the ideXlab platform.
-
the expulsion of echinostoma trivolvis worm kinetics and intestinal cytopathology in conventional and congenitally athymic balb c mice
Parasitology, 1993Co-Authors: Takahiro Fujino, Bernard Fried, Isao TadaAbstract:: The infectivity and distribution of Echinostoma trivolvis were studied in male, conventional and congenitally athymic nude mice, each infected with 30 metacercarial cysts. In conventional mice, worm recoveries at 6 and 8 days post-exposure were 58.3 and 54.0%, respectively. Worm recovery declined to 44.0% by day 10, to 4.3% by day 13, and 0% by day 17. In athymic mice, worm recoveries at 6 and 8 days post-exposure were 61.7 and 36.3%, respectively. Worm recovery declined to 27.7% by day 10, to 0.7% by day 13, and 0% by day 17. The distribution of worms demonstrated a posteriad migration over time in both groups. Kinetic changes in the number of goblet and mucosal mast cells in the Upper Ileum of mice infected with E. trivolvis were examined. In conventional mice, the number of goblet cells increased rapidly to reach a peak at day 13 and then declined gradually. The number of goblet cells in athymic mice also increased to reach a peak at day 13, and then declined rapidly. However, the number of goblet cells in athymic mice was always less than that in conventional mice. The mast cell number in infected conventional mice increased rapidly to reach a peak at day 17 and then declined. There was no increase in the mast cell number of infected athymic mice throughout the experiment. Whereas common pathological changes occurred in the intestines of both mice groups infected with echinostomes some ultrastructural differences were observed in the gut epithelial cells of conventional versus athymic mice.
Takahiro Fujino - One of the best experts on this subject based on the ideXlab platform.
-
the expulsion of echinostoma trivolvis worm kinetics and intestinal cytopathology in conventional and congenitally athymic balb c mice
Parasitology, 1993Co-Authors: Takahiro Fujino, Bernard Fried, Isao TadaAbstract:: The infectivity and distribution of Echinostoma trivolvis were studied in male, conventional and congenitally athymic nude mice, each infected with 30 metacercarial cysts. In conventional mice, worm recoveries at 6 and 8 days post-exposure were 58.3 and 54.0%, respectively. Worm recovery declined to 44.0% by day 10, to 4.3% by day 13, and 0% by day 17. In athymic mice, worm recoveries at 6 and 8 days post-exposure were 61.7 and 36.3%, respectively. Worm recovery declined to 27.7% by day 10, to 0.7% by day 13, and 0% by day 17. The distribution of worms demonstrated a posteriad migration over time in both groups. Kinetic changes in the number of goblet and mucosal mast cells in the Upper Ileum of mice infected with E. trivolvis were examined. In conventional mice, the number of goblet cells increased rapidly to reach a peak at day 13 and then declined gradually. The number of goblet cells in athymic mice also increased to reach a peak at day 13, and then declined rapidly. However, the number of goblet cells in athymic mice was always less than that in conventional mice. The mast cell number in infected conventional mice increased rapidly to reach a peak at day 17 and then declined. There was no increase in the mast cell number of infected athymic mice throughout the experiment. Whereas common pathological changes occurred in the intestines of both mice groups infected with echinostomes some ultrastructural differences were observed in the gut epithelial cells of conventional versus athymic mice.
