Ureter Obstruction

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Jørgen Frøkiær - One of the best experts on this subject based on the ideXlab platform.

  • Renal water and sodium handling during gradated unilateral Ureter Obstruction.
    Scandinavian Journal of Urology and Nephrology, 2009
    Co-Authors: T. S. Pedersen, Jens Christian Djurhuus, Jørgen Frøkiær
    Abstract:

    Objective: Unilateral complete Obstruction of the Ureter (UUO) is associated with characteristic changes in renal function. To improve the understanding of how urine concentration directly is affected by changes in pelvic pressure, changes in renal salt and water handling along the nephron and collecting duct were examined. Material and Methods: Pelvic pressure was raised stepwise using an adjustable lever inserted in the right Ureter. Urine samples were collected from the tip of the catheter by way of an overflow system. Water and sodium handling in the distal and proximal tubules were measured by the lithium clearance technique. Renal blood flow (RBF) was measured with implanted ultrasonic flow probes. Catheters were placed in both renal veins and glomerular filtration rate (GFR) and filtration fraction were calculated using renal extraction of 51 Cr-EDTA independent of urine sampling. Results: The sequence of changes for each parameter is provided. The parameters did not show a uniform pattern from whi...

  • Age-dependent renal expression of acid-base transporters in neonatal Ureter Obstruction.
    Pediatric Nephrology, 2009
    Co-Authors: Guixian Wang, Jianguo Wen, Jens Christian Djurhuus, Sukru Oguzkan Topcu, Troels Ring, Tae-hwan Kwon, Søren Nielsen, Jørgen Frøkiær
    Abstract:

    Congenital obstructive nephropathy accounts for a major proportion of renal insufficiency in infancy and childhood. In an earlier investigation we demonstrated that bilateral complete Ureteral Obstruction (BUO) in rats is associated with inadequate urinary acidification [Am J Physiol Renal Physiol. 295(2):F497-506, 2008]. The aim of the study reported here was to determine whether this defect is also associated with unilateral Ureteral Obstruction (UUO), which is clinically more common than BUO. The time-course of the changes in protein expression levels of major renal acid–base transporters was examined at 7 and 14 weeks in rats with neonatally induced partial unilateral Ureteral Obstruction (PUUO), which was performed within the first 48 h of life. We observed that protein expression of the renal acid–base transporters NHE3, NBC1, NBCn1, pendrin and Na+-K+-ATPase was increased in both obstructed and non-obstructed kidneys 7 weeks after the induction of neonatal PUUO. This was confirmed by immunocytochemistry. In contrast, 14 weeks after the induction of PUUO, there was a significant downregulation of the renal acid–base transporters NBC1, NBCn1 and Na+-K+-ATPase in the obstructed kidneys. These time/age-dependent changes in protein expression were associated with parallel changes in renal function resulting in urine acidification in response to exogenous acid loading. In conclusion, these results show that downregulation of protein expression is a time/age-dependent response to PUUO, which could contribute to the decreased net acid excretion and development of metabolic acidosis in neonatal rats with PUUO.

  • Renal hemodynamic response to gradated Ureter Obstruction in the pig
    Nephron, 2008
    Co-Authors: Jan J Hvistendahl, Thomas Pedersen, Hans H. Jørgensen, Michael Rehling, Jørgen Frøkiær
    Abstract:

    In anesthetized pigs with ultrasonic flow probes mounted on each renal artery and catheters placed in the abdominal aorta and both renal veins, renal blood flow (RBF), glomerular filtration rate (GFR)

  • Ureter Obstruction alters expression of renal acid-base transport proteins in rat kidney
    American Journal of Physiology-renal Physiology, 2008
    Co-Authors: Guixian Wang, Jianguo Wen, Jens Christian Djurhuus, Troels Ring, Søren Nielsen, Soo Wan Kim, Weidong Wang, Jørgen Frøkiær
    Abstract:

    Urinary tract Obstruction impairs renal function and is often associated with a urinary acidification defect caused by diminished net H+ secretion and/or HCO3− reabsorption. To identify the molecul...

  • reduced renal vascular resistance in response to verapamil during gradated Ureter Obstruction in pigs
    Urological Research, 2001
    Co-Authors: Jan J Hvistendahl, Thomas Pedersen, Gitte M Hvistendahl, J C Djurhuus, Jørgen Frøkiær
    Abstract:

    Unilateral Ureteral Obstruction (UUO) is associated with reductions in ipsilateral renal blood flow (RBF) and glomerular filtration rate (GFR) caused by an active preglomerular vasoconstriction, where angiotensin II (ANGII) may be an important mediator. Calcium-channel blockers preferentially dilate preglomerular vessels and abolish the vasoconstrictor actions of ANGII in preglomerular arterioles of the hydronephrotic rat kidney. In this study, we, therefore, examined the effects of the calcium-channel blocker verapamil (3.65 µg/kg per minute i.v.) on RBF, GFR and renal vascular resistance (RVR) in our pig model with UUO, where ultrasonic flow probes are mounted on each renal artery and catheters placed in the abdominal aorta and both renal veins. Verapamil treatment was associated with a 34% reduction in ipsilateral RBF (from 182.6±20.5 ml/min to 120.6±12.2 ml/min, P<0.001), which was similar to the 27% reduction in ipsilateral RBF in controls (from 194.6±13.1 ml/min to 140.6±15.2 ml/min, P<0.001). Ipsilateral GFR was reduced by 70% in the verapamil-treated pigs (from 29.0±2.6 to 8.5±0.9 ml/min, P<0.001) and by 73% in control animals (from 29.2±3.1 to 7.6±2.1 ml/min, p<0.001). However, the increase in RVR was significantly attenuated in the verapamil-treated pigs. Ipsilateral RVR increased by 19% in the verapamil-treated pigs (from 0.585±0.076 to 0.726±0.081 mmHg/min/ml, P<0.05) compared with a 34% increase in control pigs (from 0.560±0.056 to 0.854±0.091 mmHg/min per milliliter, P<0.001), suggesting that an intact calcium-channel may be important for the increase in renal vascular resistance during unilateral Ureter Obstruction. In conclusion, the present study shows that verapamil is able to modulate the increase in renal vascular resistance in response to increased pelvic pressure.

Jens Christian Djurhuus - One of the best experts on this subject based on the ideXlab platform.

  • Renal water and sodium handling during gradated unilateral Ureter Obstruction.
    Scandinavian Journal of Urology and Nephrology, 2009
    Co-Authors: T. S. Pedersen, Jens Christian Djurhuus, Jørgen Frøkiær
    Abstract:

    Objective: Unilateral complete Obstruction of the Ureter (UUO) is associated with characteristic changes in renal function. To improve the understanding of how urine concentration directly is affected by changes in pelvic pressure, changes in renal salt and water handling along the nephron and collecting duct were examined. Material and Methods: Pelvic pressure was raised stepwise using an adjustable lever inserted in the right Ureter. Urine samples were collected from the tip of the catheter by way of an overflow system. Water and sodium handling in the distal and proximal tubules were measured by the lithium clearance technique. Renal blood flow (RBF) was measured with implanted ultrasonic flow probes. Catheters were placed in both renal veins and glomerular filtration rate (GFR) and filtration fraction were calculated using renal extraction of 51 Cr-EDTA independent of urine sampling. Results: The sequence of changes for each parameter is provided. The parameters did not show a uniform pattern from whi...

  • Age-dependent renal expression of acid-base transporters in neonatal Ureter Obstruction.
    Pediatric Nephrology, 2009
    Co-Authors: Guixian Wang, Jianguo Wen, Jens Christian Djurhuus, Sukru Oguzkan Topcu, Troels Ring, Tae-hwan Kwon, Søren Nielsen, Jørgen Frøkiær
    Abstract:

    Congenital obstructive nephropathy accounts for a major proportion of renal insufficiency in infancy and childhood. In an earlier investigation we demonstrated that bilateral complete Ureteral Obstruction (BUO) in rats is associated with inadequate urinary acidification [Am J Physiol Renal Physiol. 295(2):F497-506, 2008]. The aim of the study reported here was to determine whether this defect is also associated with unilateral Ureteral Obstruction (UUO), which is clinically more common than BUO. The time-course of the changes in protein expression levels of major renal acid–base transporters was examined at 7 and 14 weeks in rats with neonatally induced partial unilateral Ureteral Obstruction (PUUO), which was performed within the first 48 h of life. We observed that protein expression of the renal acid–base transporters NHE3, NBC1, NBCn1, pendrin and Na+-K+-ATPase was increased in both obstructed and non-obstructed kidneys 7 weeks after the induction of neonatal PUUO. This was confirmed by immunocytochemistry. In contrast, 14 weeks after the induction of PUUO, there was a significant downregulation of the renal acid–base transporters NBC1, NBCn1 and Na+-K+-ATPase in the obstructed kidneys. These time/age-dependent changes in protein expression were associated with parallel changes in renal function resulting in urine acidification in response to exogenous acid loading. In conclusion, these results show that downregulation of protein expression is a time/age-dependent response to PUUO, which could contribute to the decreased net acid excretion and development of metabolic acidosis in neonatal rats with PUUO.

  • Ureter Obstruction alters expression of renal acid-base transport proteins in rat kidney
    American Journal of Physiology-renal Physiology, 2008
    Co-Authors: Guixian Wang, Jianguo Wen, Jens Christian Djurhuus, Troels Ring, Søren Nielsen, Soo Wan Kim, Weidong Wang, Jørgen Frøkiær
    Abstract:

    Urinary tract Obstruction impairs renal function and is often associated with a urinary acidification defect caused by diminished net H+ secretion and/or HCO3− reabsorption. To identify the molecul...

  • Reduced renal vascular resistance in response to verapamil during gradated Ureter Obstruction in pigs.
    Urological Research, 2001
    Co-Authors: Jan J Hvistendahl, Jens Christian Djurhuus, Thomas Pedersen, Gitte M Hvistendahl, Jørgen Frøkiær
    Abstract:

    Unilateral Ureteral Obstruction (UUO) is associated with reductions in ipsilateral renal blood flow (RBF) and glomerular filtration rate (GFR) caused by an active preglomerular vasoconstriction, where angiotensin II (ANGII) may be an important mediator. Calcium-channel blockers preferentially dilate preglomerular vessels and abolish the vasoconstrictor actions of ANGII in preglomerular arterioles of the hydronephrotic rat kidney. In this study, we, therefore, examined the effects of the calcium-channel blocker verapamil (3.65 µg/kg per minute i.v.) on RBF, GFR and renal vascular resistance (RVR) in our pig model with UUO, where ultrasonic flow probes are mounted on each renal artery and catheters placed in the abdominal aorta and both renal veins. Verapamil treatment was associated with a 34% reduction in ipsilateral RBF (from 182.6±20.5 ml/min to 120.6±12.2 ml/min, P

  • EXPERIMENTAL PARTIAL UNILATERAL Ureter Obstruction. I. PRESSURE FLOW RELATIONSHIP IN A RAT MODEL WITH MILD AND SEVERE ACUTE Ureter Obstruction
    The Journal of Urology, 1998
    Co-Authors: Jianguo Wen, Yue Chen, Jørgen Frøkiær, Troels Munch Jørgensen, Jens Christian Djurhuus
    Abstract:

    AbstractPurpose: To create an animal model with mild and severe partial unilateral Ureter Obstruction in young rats using a modified Ulm and Miller technique and to characterize the model by acute renal pelvic pressure measurements.Material and Methods: During anesthesia the upper fourth (n = 15) or the upper two-thirds of the left Ureter (n = 15) was embedded into the psoas muscle causing either a mild or severe partial Obstruction. Sham-operated control rats were prepared in paralllel (n = 20). The baseline pelvic pressure, the perfusion pelvic pressure (perfusion rates: 0.2 to 1.0 ml. per minute) and peristaltic waves were recorded after a resting period.Results: Mean baseline pelvic pressure and perfusion pelvic pressure were significantly higher in obstructed kidneys than in non-obstructed kidneys, and significantly higher in severely obstructed kidneys than in mildly obstructed kidneys (p

Jianguo Wen - One of the best experts on this subject based on the ideXlab platform.

  • effect of erythropoietin on the expression and function of renal aquaporin 2 after release of bilateral Ureter Obstruction in young rats
    Chinese Journal of Applied Clinical Pediatrics, 2016
    Co-Authors: Yan Wang, Jianguo Wen, Chuanchuan Ren, Li Yang, Yutao Lyu, Jiafeng Xie, Shuman Huang, Zhiming Jia, Qingwei Wang
    Abstract:

    Objective To investigate the effect of erythropoietin (EPO) on the expression of aquaporin-2 (AQP2) in the kidney of young SD rats after release of bilateral Ureter Obstruction (BUO-R). Methods Thirty-two young SD rats were equally divided into 4 groups randomly (BUO group, BUO-R group, BUO-R+ EPO group and Sham group, 8 rats in each group). The BUO model was built through bilateral Ureteral ligation.EPO (500 U/kg) was given to BUO-R+ EPO rats at 2 h after release of BUO, and then repeated 6 h, 12 h, 24 h and 36 h thereafter and the same volume of 9 g/L saline was simultaneously given to BUO-R rats.The Sham group was prepared in parallel by laparotomy and free dissection of bilateral Ureters but not ligated.Both side kidneys were harvested 48 h (72 h for Sham group) after release of BUO to examine the effect of EPO on the expression of AQP2 in inner medulla by immunohistochemistry, Real-time PCR and Western blot.The urine samples were collected by using metabolic cage before death. Results The osmotic pressure of BUO-R+ EPO group was higher than that of BUO-R group, but lower than that of Sham group (P<0.05). Immunohistochemistry showed that the collecting duct wall thinned and lumen enlarged.After the pictures were analysized by using Image-Pro Plus software, it showed that the expression of AQP2 in collecting duct in BUO group was significantly down-regulated compared with that in Sham group, whereas, it was slightly weaker in BUO-R group and BUO-R+ EPO group than Sham group(P<0.05). These results were further confirmed by adopting Western blot, and the relative quantity of AQP2 in BUO group was also the lowest of the four groups (P<0.05). Real-time PCR showed that the level of AQP2 mRNA in Sham group was (24.30±1.03) folds of BUO group, (10.60±1.05) folds of BUO-R group and (5.70±1.01) folds of BUO-R+ EPO group, respectively. Conclusion EPO could promote not only the recovery of AQP2 mRNA and protein expression but also the recovery of AQP2 function in young BUO-R rats. Key words: Erythropoietin; Ureter Obstruction; Aquaporin-2; Kidney

  • Effect of erythropoietin on the expression and function of renal aquaporin-2 after release of bilateral Ureter Obstruction in young rats
    Chinese Journal of Applied Clinical Pediatrics, 2016
    Co-Authors: Yan Wang, Jianguo Wen, Chuanchuan Ren, Li Yang, Yutao Lyu, Jiafeng Xie, Shuman Huang, Zhiming Jia, Qingwei Wang
    Abstract:

    Objective To investigate the effect of erythropoietin (EPO) on the expression of aquaporin-2 (AQP2) in the kidney of young SD rats after release of bilateral Ureter Obstruction (BUO-R). Methods Thirty-two young SD rats were equally divided into 4 groups randomly (BUO group, BUO-R group, BUO-R+ EPO group and Sham group, 8 rats in each group). The BUO model was built through bilateral Ureteral ligation.EPO (500 U/kg) was given to BUO-R+ EPO rats at 2 h after release of BUO, and then repeated 6 h, 12 h, 24 h and 36 h thereafter and the same volume of 9 g/L saline was simultaneously given to BUO-R rats.The Sham group was prepared in parallel by laparotomy and free dissection of bilateral Ureters but not ligated.Both side kidneys were harvested 48 h (72 h for Sham group) after release of BUO to examine the effect of EPO on the expression of AQP2 in inner medulla by immunohistochemistry, Real-time PCR and Western blot.The urine samples were collected by using metabolic cage before death. Results The osmotic pressure of BUO-R+ EPO group was higher than that of BUO-R group, but lower than that of Sham group (P

  • effect of erythropoietin on expression of aquaporin 2 in kidney of young rats with partial unilateral Ureter Obstruction
    Chinese Journal of Applied Clinical Pediatrics, 2013
    Co-Authors: Jianguo Wen, Chuanchuan Ren, L Yutao, Lu Xing, Li Yang, Liu Zhenzhen, Yan Wang, S U Zhiqiang, Mingliang Xia, Qingwei Wang
    Abstract:

    Objective To investigate the effect of erythropoietin (EPO) on expression of aquaporin-2 (AQP2) in the kidney of young SD rats with partial unilateral Ureter Obstruction (PUUO). Methods Twenty-four young SD rats were randomly divided into 3 groups (PUUO group, n=8; PUUO+ EPO group, n=8; sham group, n=8). The PUUO model was built by embedding the upper quarter of the left Ureter into psoas muscle.EPO(500 U/kg) was given to PUUO+ EPO rats at 1 h after surgery, and then repeated 1, 3, 5 days later after surgery by intraperitoneal injection and the same volume of 9 g/L saline was simultaneously given to PUUO rats.The sham group was prepared in parallel by way of laparotomy and free dissection of the left Ureter.In 1 week after surgery, the left kidneys of rats were harvested to examine the effect of EPO on the expression of AQP2 in inner medulla by immunohistochemistry, real-time PCR and Western blot. Results Immunohistochemistry showed that the expression of AQP2 in the collecting duct in PUUO group was significantly downregulated than those in sham group and PUUO+ EPO group, whereas, it was slightly weaker in PUUO+ EPO group than those in sham group.These results were further confirmed by Western blot, and the relative quantity of AQP2 in PUUO group was also lower than those in PUUO+ EPO group and sham group (0.290±0.016 vs 0.640±0.106 vs 0.840±0.134, P<0.05). Real-time PCR showed that the level of AQP2 mRNA in PUUO group was lower than those in PUUO+ EPO group and sham group (ΔCt values of 3 groups were -7.60±0.18 vs -9.70±0.54 vs -10.80±0.31, P<0.05). Conclusions EPO can partially prevent the downregulation of AQP2 in young PUUO rats, and its mechanism needs to be further investigated. Key words: Erythropoietin; Partial unilateral Ureter Obstruction; Aquaporin-2; Kidney

  • Effect of erythropoietin on expression of aquaporin-2 in kidney of young rats with partial unilateral Ureter Obstruction
    Chinese Journal of Applied Clinical Pediatrics, 2013
    Co-Authors: Jianguo Wen, Chuanchuan Ren, Lu Xing, Li Yang, Liu Zhenzhen, Yan Wang, Mingliang Xia, Qingwei Wang
    Abstract:

    Objective To investigate the effect of erythropoietin (EPO) on expression of aquaporin-2 (AQP2) in the kidney of young SD rats with partial unilateral Ureter Obstruction (PUUO). Methods Twenty-four young SD rats were randomly divided into 3 groups (PUUO group, n=8; PUUO+ EPO group, n=8; sham group, n=8). The PUUO model was built by embedding the upper quarter of the left Ureter into psoas muscle.EPO(500 U/kg) was given to PUUO+ EPO rats at 1 h after surgery, and then repeated 1, 3, 5 days later after surgery by intraperitoneal injection and the same volume of 9 g/L saline was simultaneously given to PUUO rats.The sham group was prepared in parallel by way of laparotomy and free dissection of the left Ureter.In 1 week after surgery, the left kidneys of rats were harvested to examine the effect of EPO on the expression of AQP2 in inner medulla by immunohistochemistry, real-time PCR and Western blot. Results Immunohistochemistry showed that the expression of AQP2 in the collecting duct in PUUO group was significantly downregulated than those in sham group and PUUO+ EPO group, whereas, it was slightly weaker in PUUO+ EPO group than those in sham group.These results were further confirmed by Western blot, and the relative quantity of AQP2 in PUUO group was also lower than those in PUUO+ EPO group and sham group (0.290±0.016 vs 0.640±0.106 vs 0.840±0.134, P

  • Age-dependent renal expression of acid-base transporters in neonatal Ureter Obstruction.
    Pediatric Nephrology, 2009
    Co-Authors: Guixian Wang, Jianguo Wen, Jens Christian Djurhuus, Sukru Oguzkan Topcu, Troels Ring, Tae-hwan Kwon, Søren Nielsen, Jørgen Frøkiær
    Abstract:

    Congenital obstructive nephropathy accounts for a major proportion of renal insufficiency in infancy and childhood. In an earlier investigation we demonstrated that bilateral complete Ureteral Obstruction (BUO) in rats is associated with inadequate urinary acidification [Am J Physiol Renal Physiol. 295(2):F497-506, 2008]. The aim of the study reported here was to determine whether this defect is also associated with unilateral Ureteral Obstruction (UUO), which is clinically more common than BUO. The time-course of the changes in protein expression levels of major renal acid–base transporters was examined at 7 and 14 weeks in rats with neonatally induced partial unilateral Ureteral Obstruction (PUUO), which was performed within the first 48 h of life. We observed that protein expression of the renal acid–base transporters NHE3, NBC1, NBCn1, pendrin and Na+-K+-ATPase was increased in both obstructed and non-obstructed kidneys 7 weeks after the induction of neonatal PUUO. This was confirmed by immunocytochemistry. In contrast, 14 weeks after the induction of PUUO, there was a significant downregulation of the renal acid–base transporters NBC1, NBCn1 and Na+-K+-ATPase in the obstructed kidneys. These time/age-dependent changes in protein expression were associated with parallel changes in renal function resulting in urine acidification in response to exogenous acid loading. In conclusion, these results show that downregulation of protein expression is a time/age-dependent response to PUUO, which could contribute to the decreased net acid excretion and development of metabolic acidosis in neonatal rats with PUUO.

Qingwei Wang - One of the best experts on this subject based on the ideXlab platform.

  • Protective effect of erythropoietin on renal function after releasing of bilateral Ureter Obstruction in rats and mechanism
    Chinese journal of experimental surgery, 2017
    Co-Authors: Yan Wang, Chuanchuan Ren, Xuepei Zhang, Yutao Lyu, Jiafeng Xie, Qingwei Wang
    Abstract:

    Objective To investigate the mechanism of protective effect of erythropoietin (EPO) on renal function after releasing of bilateral Ureter Obstruction (BUO) in SD rats. Methods Thirty-two SD rats were divided into 4 groups randomly (BUO group, BUO-R group, BUO-R+ EPO group and Sham group; n=8). The BUO model was built by bilateral Ureteral ligation. EPO was given to BUO-R+ EPO rats 1 h after releasing of BUO, and then repeated 12, 24, 36, 48 h thereafter and the same volume of normal saline was simultaneously given to BUO-R rats. The urine samples were collected 24 h before death and then blood samples were collected from inferior vena cava. The kidneys at both sides were harvested 72 h after releasing of BUO to examine the effect of EPO on the expression of tumor necrosis factor-α (TNF-α) and interleukin (IL)-6 by immunohistochemistry and real-time quantitative polymerase chain reaction (Real-time PCR). Results The osmotic pressure in BUO-R+ EPO group was higher than that in BUO-R group, while lower than that in Sham group [(1 164±93) vs. (715±76) vs. (1 835±98) mosm/kg, P=0.000]. The hematal concentrations of urea and CREA in BUO group [(58.40±4.63) mmol/L and (277.30±11.26) μmol/L] were higher than those in other three groups, while they were higher in BUO-R+ EPO group [(12.20±2.34) mmol/L and (34.70±4.58) μmol/L] than those in Sham group [(6.40±0.75) mmol/L and (20.50±3.71) μmol/L], but lower than those in BUO-R group [(25.70±3.26) mmol/L and (52.60±7.83) μmol/L], P=0.000. Immunohistochemistry showed the expression levels of TNF-α and IL-6 were strongest in BUO group and weakest in Sham group, while those were stronger in BUO-R group than in BUO-R+ EPO group. Real-time PCR showed that the expression of TNF-α and IL-6 mRNA in BUO group was the most, while that in Sham group was the least, and that in BUO-R+ EPO group was less than in BUO-R group. Conclusion EPO could promote the recovery of renal function by inhibiting inflammatory reaction in BUO-R rats. Key words: Erythropoietin; Ureter Obstruction; Kidney; Inflammatory reaction

  • the protective effect of erythropoietin on renal function after releasing of bilateral Ureter Obstruction in young rat
    Chinese Journal of Urology, 2016
    Co-Authors: Yan Wang, Chuanchuan Ren, Qingwei Wang, Yutao Lyu, Jiafeng Xie, Lingang Cui, Wen Zhu
    Abstract:

    Objective To investigate the protective effect of erythropoietin (EPO) on renal function after releasing of bilateral Ureters Obstruction (BUO-R) in young SD rats. Methods From October 2015 to June 2016, forty young SD rats were bought and equally divided into 4 groups randomly (BUO group, BUO-R group, BUO-R+ EPO group and Sham group; n=10) according to random number table. The BUO model was built by bilateral Ureteral ligation. EPO (500 U/kg) was given to BUO-R+ EPO rats immediately after releasing of BUO, and then repeated 2d, 4d and 6d thereafter and the same volume of normal saline was simultaneously given to BUO-R rats. The Sham group was prepared in parallel by laparotomy and free dissection of bilateral Ureters but not ligated. The urine samples were collected by metabolic cage 24h before death and then blood samples (1 ml) were collected from inferior vena cava. Results The urine osmolarity of Sham group was the highest [(1 794±103)mOsm/kg], BUO-R+ EPO group was (1 257±82)mOsm/kg, and BUO-R group was the lowest [(756±69)mOsm/kg]; Whereas the urine output of Sham group was the lowest [(26±5)μl/(min·kg)], BUO-R+ EPO group was (48±7)μl/(min·kg), and BUO-R group was the highest [(71±9)μl/(min·kg)]; P<0.05. The concentrations of urinary K+ , Na+ and creatinine in BUO-R+ EPO group were [(133.8±5.8)mmol/L, (134.7±4.9)mmol/L and (3 549±482)μmol/L]lower than those in Sham group [(154.7±6.5)mmol/L, (149.3±7.8)mmol/L and (4 537±624)μmol/L], but higher than those in BUO-R group [(112.4±7.2)mmol/L, (106.5±6.3)mmol/L and (2 612±536)μmol/L], P<0.05. The concentrations of hematal K+ , urea and creatinine in three experimental groups were all higher than those in Sham group; of which the hematal K+ , urea and creatinine in BUO group were the highest[(12.1±0.7)mmol/L, (47.6±6.0)mmol/L and (252.8±18.7)μmol/L], the BUO-R group took the second place [(8.5±0.5)mmol/L, (7.8±0.4)mmol/L and (55.2±4.8)μmol/L], and the BUO-R+ EPO group came third [(7.4±0.5)mmol/L, (6.4±0.3)mmol/L and (36.4±3.6)μmol/L], while the Sham group were the lowest [(6.8±0.3)mmol/L, (5.6±0.24)mmol/L and (23.6±2.7)μmol/L]; P<0.05. While the hematal Na+ concentration in BUO group was the lowest [(126.4±5.9)mmol/L], and BUO-R group was the highest [(147.3±4.9)mmol/L], the Sham group was the second lowest [(136.7±4.3)mmol/L], and the BUO-R+ EPO group was the second highest [(141.5±5.7)mmol/L], P<0.05. Conclusion EPO could promote the recovery of renal function and normalize the altered renal water and salt handling function in young BUO-R rats. Key words: Erythropoietin; Ureter Obstruction; Renal function

  • The protective effect of erythropoietin on renal function after releasing of bilateral Ureter Obstruction in young rat
    Chinese Journal of Urology, 2016
    Co-Authors: Yan Wang, Chuanchuan Ren, Qingwei Wang, Yutao Lyu, Jiafeng Xie, Lingang Cui, Wen Zhu
    Abstract:

    Objective To investigate the protective effect of erythropoietin (EPO) on renal function after releasing of bilateral Ureters Obstruction (BUO-R) in young SD rats. Methods From October 2015 to June 2016, forty young SD rats were bought and equally divided into 4 groups randomly (BUO group, BUO-R group, BUO-R+ EPO group and Sham group; n=10) according to random number table. The BUO model was built by bilateral Ureteral ligation. EPO (500 U/kg) was given to BUO-R+ EPO rats immediately after releasing of BUO, and then repeated 2d, 4d and 6d thereafter and the same volume of normal saline was simultaneously given to BUO-R rats. The Sham group was prepared in parallel by laparotomy and free dissection of bilateral Ureters but not ligated. The urine samples were collected by metabolic cage 24h before death and then blood samples (1 ml) were collected from inferior vena cava. Results The urine osmolarity of Sham group was the highest [(1 794±103)mOsm/kg], BUO-R+ EPO group was (1 257±82)mOsm/kg, and BUO-R group was the lowest [(756±69)mOsm/kg]; Whereas the urine output of Sham group was the lowest [(26±5)μl/(min·kg)], BUO-R+ EPO group was (48±7)μl/(min·kg), and BUO-R group was the highest [(71±9)μl/(min·kg)]; P

  • effect of erythropoietin on the expression and function of renal aquaporin 2 after release of bilateral Ureter Obstruction in young rats
    Chinese Journal of Applied Clinical Pediatrics, 2016
    Co-Authors: Yan Wang, Jianguo Wen, Chuanchuan Ren, Li Yang, Yutao Lyu, Jiafeng Xie, Shuman Huang, Zhiming Jia, Qingwei Wang
    Abstract:

    Objective To investigate the effect of erythropoietin (EPO) on the expression of aquaporin-2 (AQP2) in the kidney of young SD rats after release of bilateral Ureter Obstruction (BUO-R). Methods Thirty-two young SD rats were equally divided into 4 groups randomly (BUO group, BUO-R group, BUO-R+ EPO group and Sham group, 8 rats in each group). The BUO model was built through bilateral Ureteral ligation.EPO (500 U/kg) was given to BUO-R+ EPO rats at 2 h after release of BUO, and then repeated 6 h, 12 h, 24 h and 36 h thereafter and the same volume of 9 g/L saline was simultaneously given to BUO-R rats.The Sham group was prepared in parallel by laparotomy and free dissection of bilateral Ureters but not ligated.Both side kidneys were harvested 48 h (72 h for Sham group) after release of BUO to examine the effect of EPO on the expression of AQP2 in inner medulla by immunohistochemistry, Real-time PCR and Western blot.The urine samples were collected by using metabolic cage before death. Results The osmotic pressure of BUO-R+ EPO group was higher than that of BUO-R group, but lower than that of Sham group (P<0.05). Immunohistochemistry showed that the collecting duct wall thinned and lumen enlarged.After the pictures were analysized by using Image-Pro Plus software, it showed that the expression of AQP2 in collecting duct in BUO group was significantly down-regulated compared with that in Sham group, whereas, it was slightly weaker in BUO-R group and BUO-R+ EPO group than Sham group(P<0.05). These results were further confirmed by adopting Western blot, and the relative quantity of AQP2 in BUO group was also the lowest of the four groups (P<0.05). Real-time PCR showed that the level of AQP2 mRNA in Sham group was (24.30±1.03) folds of BUO group, (10.60±1.05) folds of BUO-R group and (5.70±1.01) folds of BUO-R+ EPO group, respectively. Conclusion EPO could promote not only the recovery of AQP2 mRNA and protein expression but also the recovery of AQP2 function in young BUO-R rats. Key words: Erythropoietin; Ureter Obstruction; Aquaporin-2; Kidney

  • Effect of erythropoietin on the expression and function of renal aquaporin-2 after release of bilateral Ureter Obstruction in young rats
    Chinese Journal of Applied Clinical Pediatrics, 2016
    Co-Authors: Yan Wang, Jianguo Wen, Chuanchuan Ren, Li Yang, Yutao Lyu, Jiafeng Xie, Shuman Huang, Zhiming Jia, Qingwei Wang
    Abstract:

    Objective To investigate the effect of erythropoietin (EPO) on the expression of aquaporin-2 (AQP2) in the kidney of young SD rats after release of bilateral Ureter Obstruction (BUO-R). Methods Thirty-two young SD rats were equally divided into 4 groups randomly (BUO group, BUO-R group, BUO-R+ EPO group and Sham group, 8 rats in each group). The BUO model was built through bilateral Ureteral ligation.EPO (500 U/kg) was given to BUO-R+ EPO rats at 2 h after release of BUO, and then repeated 6 h, 12 h, 24 h and 36 h thereafter and the same volume of 9 g/L saline was simultaneously given to BUO-R rats.The Sham group was prepared in parallel by laparotomy and free dissection of bilateral Ureters but not ligated.Both side kidneys were harvested 48 h (72 h for Sham group) after release of BUO to examine the effect of EPO on the expression of AQP2 in inner medulla by immunohistochemistry, Real-time PCR and Western blot.The urine samples were collected by using metabolic cage before death. Results The osmotic pressure of BUO-R+ EPO group was higher than that of BUO-R group, but lower than that of Sham group (P

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  • Protective effect of erythropoietin on renal function after releasing of bilateral Ureter Obstruction in rats and mechanism
    Chinese journal of experimental surgery, 2017
    Co-Authors: Yan Wang, Chuanchuan Ren, Xuepei Zhang, Yutao Lyu, Jiafeng Xie, Qingwei Wang
    Abstract:

    Objective To investigate the mechanism of protective effect of erythropoietin (EPO) on renal function after releasing of bilateral Ureter Obstruction (BUO) in SD rats. Methods Thirty-two SD rats were divided into 4 groups randomly (BUO group, BUO-R group, BUO-R+ EPO group and Sham group; n=8). The BUO model was built by bilateral Ureteral ligation. EPO was given to BUO-R+ EPO rats 1 h after releasing of BUO, and then repeated 12, 24, 36, 48 h thereafter and the same volume of normal saline was simultaneously given to BUO-R rats. The urine samples were collected 24 h before death and then blood samples were collected from inferior vena cava. The kidneys at both sides were harvested 72 h after releasing of BUO to examine the effect of EPO on the expression of tumor necrosis factor-α (TNF-α) and interleukin (IL)-6 by immunohistochemistry and real-time quantitative polymerase chain reaction (Real-time PCR). Results The osmotic pressure in BUO-R+ EPO group was higher than that in BUO-R group, while lower than that in Sham group [(1 164±93) vs. (715±76) vs. (1 835±98) mosm/kg, P=0.000]. The hematal concentrations of urea and CREA in BUO group [(58.40±4.63) mmol/L and (277.30±11.26) μmol/L] were higher than those in other three groups, while they were higher in BUO-R+ EPO group [(12.20±2.34) mmol/L and (34.70±4.58) μmol/L] than those in Sham group [(6.40±0.75) mmol/L and (20.50±3.71) μmol/L], but lower than those in BUO-R group [(25.70±3.26) mmol/L and (52.60±7.83) μmol/L], P=0.000. Immunohistochemistry showed the expression levels of TNF-α and IL-6 were strongest in BUO group and weakest in Sham group, while those were stronger in BUO-R group than in BUO-R+ EPO group. Real-time PCR showed that the expression of TNF-α and IL-6 mRNA in BUO group was the most, while that in Sham group was the least, and that in BUO-R+ EPO group was less than in BUO-R group. Conclusion EPO could promote the recovery of renal function by inhibiting inflammatory reaction in BUO-R rats. Key words: Erythropoietin; Ureter Obstruction; Kidney; Inflammatory reaction

  • the protective effect of erythropoietin on renal function after releasing of bilateral Ureter Obstruction in young rat
    Chinese Journal of Urology, 2016
    Co-Authors: Yan Wang, Chuanchuan Ren, Qingwei Wang, Yutao Lyu, Jiafeng Xie, Lingang Cui, Wen Zhu
    Abstract:

    Objective To investigate the protective effect of erythropoietin (EPO) on renal function after releasing of bilateral Ureters Obstruction (BUO-R) in young SD rats. Methods From October 2015 to June 2016, forty young SD rats were bought and equally divided into 4 groups randomly (BUO group, BUO-R group, BUO-R+ EPO group and Sham group; n=10) according to random number table. The BUO model was built by bilateral Ureteral ligation. EPO (500 U/kg) was given to BUO-R+ EPO rats immediately after releasing of BUO, and then repeated 2d, 4d and 6d thereafter and the same volume of normal saline was simultaneously given to BUO-R rats. The Sham group was prepared in parallel by laparotomy and free dissection of bilateral Ureters but not ligated. The urine samples were collected by metabolic cage 24h before death and then blood samples (1 ml) were collected from inferior vena cava. Results The urine osmolarity of Sham group was the highest [(1 794±103)mOsm/kg], BUO-R+ EPO group was (1 257±82)mOsm/kg, and BUO-R group was the lowest [(756±69)mOsm/kg]; Whereas the urine output of Sham group was the lowest [(26±5)μl/(min·kg)], BUO-R+ EPO group was (48±7)μl/(min·kg), and BUO-R group was the highest [(71±9)μl/(min·kg)]; P<0.05. The concentrations of urinary K+ , Na+ and creatinine in BUO-R+ EPO group were [(133.8±5.8)mmol/L, (134.7±4.9)mmol/L and (3 549±482)μmol/L]lower than those in Sham group [(154.7±6.5)mmol/L, (149.3±7.8)mmol/L and (4 537±624)μmol/L], but higher than those in BUO-R group [(112.4±7.2)mmol/L, (106.5±6.3)mmol/L and (2 612±536)μmol/L], P<0.05. The concentrations of hematal K+ , urea and creatinine in three experimental groups were all higher than those in Sham group; of which the hematal K+ , urea and creatinine in BUO group were the highest[(12.1±0.7)mmol/L, (47.6±6.0)mmol/L and (252.8±18.7)μmol/L], the BUO-R group took the second place [(8.5±0.5)mmol/L, (7.8±0.4)mmol/L and (55.2±4.8)μmol/L], and the BUO-R+ EPO group came third [(7.4±0.5)mmol/L, (6.4±0.3)mmol/L and (36.4±3.6)μmol/L], while the Sham group were the lowest [(6.8±0.3)mmol/L, (5.6±0.24)mmol/L and (23.6±2.7)μmol/L]; P<0.05. While the hematal Na+ concentration in BUO group was the lowest [(126.4±5.9)mmol/L], and BUO-R group was the highest [(147.3±4.9)mmol/L], the Sham group was the second lowest [(136.7±4.3)mmol/L], and the BUO-R+ EPO group was the second highest [(141.5±5.7)mmol/L], P<0.05. Conclusion EPO could promote the recovery of renal function and normalize the altered renal water and salt handling function in young BUO-R rats. Key words: Erythropoietin; Ureter Obstruction; Renal function

  • The protective effect of erythropoietin on renal function after releasing of bilateral Ureter Obstruction in young rat
    Chinese Journal of Urology, 2016
    Co-Authors: Yan Wang, Chuanchuan Ren, Qingwei Wang, Yutao Lyu, Jiafeng Xie, Lingang Cui, Wen Zhu
    Abstract:

    Objective To investigate the protective effect of erythropoietin (EPO) on renal function after releasing of bilateral Ureters Obstruction (BUO-R) in young SD rats. Methods From October 2015 to June 2016, forty young SD rats were bought and equally divided into 4 groups randomly (BUO group, BUO-R group, BUO-R+ EPO group and Sham group; n=10) according to random number table. The BUO model was built by bilateral Ureteral ligation. EPO (500 U/kg) was given to BUO-R+ EPO rats immediately after releasing of BUO, and then repeated 2d, 4d and 6d thereafter and the same volume of normal saline was simultaneously given to BUO-R rats. The Sham group was prepared in parallel by laparotomy and free dissection of bilateral Ureters but not ligated. The urine samples were collected by metabolic cage 24h before death and then blood samples (1 ml) were collected from inferior vena cava. Results The urine osmolarity of Sham group was the highest [(1 794±103)mOsm/kg], BUO-R+ EPO group was (1 257±82)mOsm/kg, and BUO-R group was the lowest [(756±69)mOsm/kg]; Whereas the urine output of Sham group was the lowest [(26±5)μl/(min·kg)], BUO-R+ EPO group was (48±7)μl/(min·kg), and BUO-R group was the highest [(71±9)μl/(min·kg)]; P

  • effect of erythropoietin on the expression and function of renal aquaporin 2 after release of bilateral Ureter Obstruction in young rats
    Chinese Journal of Applied Clinical Pediatrics, 2016
    Co-Authors: Yan Wang, Jianguo Wen, Chuanchuan Ren, Li Yang, Yutao Lyu, Jiafeng Xie, Shuman Huang, Zhiming Jia, Qingwei Wang
    Abstract:

    Objective To investigate the effect of erythropoietin (EPO) on the expression of aquaporin-2 (AQP2) in the kidney of young SD rats after release of bilateral Ureter Obstruction (BUO-R). Methods Thirty-two young SD rats were equally divided into 4 groups randomly (BUO group, BUO-R group, BUO-R+ EPO group and Sham group, 8 rats in each group). The BUO model was built through bilateral Ureteral ligation.EPO (500 U/kg) was given to BUO-R+ EPO rats at 2 h after release of BUO, and then repeated 6 h, 12 h, 24 h and 36 h thereafter and the same volume of 9 g/L saline was simultaneously given to BUO-R rats.The Sham group was prepared in parallel by laparotomy and free dissection of bilateral Ureters but not ligated.Both side kidneys were harvested 48 h (72 h for Sham group) after release of BUO to examine the effect of EPO on the expression of AQP2 in inner medulla by immunohistochemistry, Real-time PCR and Western blot.The urine samples were collected by using metabolic cage before death. Results The osmotic pressure of BUO-R+ EPO group was higher than that of BUO-R group, but lower than that of Sham group (P<0.05). Immunohistochemistry showed that the collecting duct wall thinned and lumen enlarged.After the pictures were analysized by using Image-Pro Plus software, it showed that the expression of AQP2 in collecting duct in BUO group was significantly down-regulated compared with that in Sham group, whereas, it was slightly weaker in BUO-R group and BUO-R+ EPO group than Sham group(P<0.05). These results were further confirmed by adopting Western blot, and the relative quantity of AQP2 in BUO group was also the lowest of the four groups (P<0.05). Real-time PCR showed that the level of AQP2 mRNA in Sham group was (24.30±1.03) folds of BUO group, (10.60±1.05) folds of BUO-R group and (5.70±1.01) folds of BUO-R+ EPO group, respectively. Conclusion EPO could promote not only the recovery of AQP2 mRNA and protein expression but also the recovery of AQP2 function in young BUO-R rats. Key words: Erythropoietin; Ureter Obstruction; Aquaporin-2; Kidney

  • Effect of erythropoietin on the expression and function of renal aquaporin-2 after release of bilateral Ureter Obstruction in young rats
    Chinese Journal of Applied Clinical Pediatrics, 2016
    Co-Authors: Yan Wang, Jianguo Wen, Chuanchuan Ren, Li Yang, Yutao Lyu, Jiafeng Xie, Shuman Huang, Zhiming Jia, Qingwei Wang
    Abstract:

    Objective To investigate the effect of erythropoietin (EPO) on the expression of aquaporin-2 (AQP2) in the kidney of young SD rats after release of bilateral Ureter Obstruction (BUO-R). Methods Thirty-two young SD rats were equally divided into 4 groups randomly (BUO group, BUO-R group, BUO-R+ EPO group and Sham group, 8 rats in each group). The BUO model was built through bilateral Ureteral ligation.EPO (500 U/kg) was given to BUO-R+ EPO rats at 2 h after release of BUO, and then repeated 6 h, 12 h, 24 h and 36 h thereafter and the same volume of 9 g/L saline was simultaneously given to BUO-R rats.The Sham group was prepared in parallel by laparotomy and free dissection of bilateral Ureters but not ligated.Both side kidneys were harvested 48 h (72 h for Sham group) after release of BUO to examine the effect of EPO on the expression of AQP2 in inner medulla by immunohistochemistry, Real-time PCR and Western blot.The urine samples were collected by using metabolic cage before death. Results The osmotic pressure of BUO-R+ EPO group was higher than that of BUO-R group, but lower than that of Sham group (P

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