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Herrera-cabrera B.e. - One of the best experts on this subject based on the ideXlab platform.
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VARIABLES DE RENDIMIENTO EN Vanilla Planifolia Jacks. ex Andrews
'Colegio de Postgraduados', 2018Co-Authors: Herrera-cabrera B.e.Abstract:De la producción nacional de vainilla (Vanilla Planifolia Jacks. ex Andrews), 80% corresponde a la región del Totonacapa del estado de Veracruz, México, con problemas de rendimiento (0.6 t ha-1) y fluctuación anua
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NUEVAS ÁREAS PARA EL CULTIVO DE VAINILLA (Vanilla Planifolia Jacks. ex Andrews.) EN HIDALGO, MÉXICO
'Colegio de Postgraduados', 2018Co-Authors: Herrera-cabrera B.e.Abstract:En la actualidad la producción de vainilla (Vanilla Planifolia Jacks. ex Andrews.), no es suficiente para impactar en los mercados nacionales e internacionales, y además existen limitantes sanitarias en el cultivo y caída prematura del fruto en regiones productora de Veracruz
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LA DIVERSIDAD DE VAINILLA (Vanilla Planifolia Jacks. ex Andrews) EN MÉXICO: RECURSO GENÉTICO ESTRATÉGICO PARA EL DESARROLLO RURAL
'Colegio de Postgraduados', 2018Co-Authors: Herrera-cabrera B.e.Abstract:Vainilla (Vanilla Planifolia Jacks. ex Andrews) es un recurso genético importante del trópico mexicano, sin embargo, su cultivo presenta problemas de índole social y biológic
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MACROPROYECTO VAINILLA (Vanilla Planifolia Jacks. ex Andrews); INVESTIGACIÓN INTERDISCIPLINARIA INTERINSTITUCIONAL-VINCULADA A DEMANDAS
'Colegio de Postgraduados', 2018Co-Authors: Herrera-cabrera B.e.Abstract:La producción de vainilla (Vanilla Planifolia Jacks. ex Andrews) (Orquidaceae) formalizada por el Comité Sistema Producto Nacional de la Vainilla (CSPNV) en la demanda sectorial 2012-04-190442, consideró cinco elementos estratégicos para atender la productividad integral del cultivo, y fortalecer su competitividad en México: 1) Conservación y manejo de germoplasma, 2)Caída prematura del fruto, 3) Sistemas de producción, 4) Nutrición vegetal y 5) Beneficiado y transformación
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VARIACIÓN DE AROMA EN Vanilla Planifolia Jacks. ex Andrews SILVESTRE Y CULTIVADA
'Colegio de Postgraduados', 2018Co-Authors: Herrera-cabrera B.e.Abstract:The primary gene pool of Vanilla Planifolia is located in Mexico, suggesting the existence of aromatic variation. Different varieties and wild relatives of V. Planifolia were evaluated, to determine the variation of the four phytochemicals that define the quality of the aroma in cured fruits from the region of Totonacapan Puebla-Veracruz and Oaxaca, Mexico. Phenolic compounds were quantified: vanillin, vanillic acid, p-hydroxybenzaldehyde, p-hydroxybenzoic, through high pressure liquid chromatography (HPLC). Twenty-five harvests from varieties in Puebla-Veracruz were compared, in contrast with six sites of wild populations in Oaxaca. The aromatic variation in varieties is determined by six groups that correspond to five genotypic groups, which have decreased the concentration of the three minor compounds (MC): p-hydroxybenzoic acid, vanillic acid and p-hydroxybenzaldehyde, in relation to the vanillin content. The aromatic variation in wild populations is defined by four groups, where the aroma responds to the existence of a higher concentration of minor compounds compared to vanillin.En México se localiza el acervo genético primario de Vanilla Planifolia, lo que sugiere la existencia de variación aromática. Se evaluaron diferentes cultivares y parientes silvestres de V. Planifolia, para determinar la variación de los cuatro fitoquímicos que definen la calidad del aroma en frutos beneficiados procedentes de la región del Totonacapan Puebla- Veracruz y de Oaxaca, México. Se cuantificaron compuestos fenólicos: vainillina, ácido vaníllico, p-hidroxibenzaldehído, p-hidroxibenzoico, mediante cromatografía líquida de alta presión (HPLC). Se compararon 25 recolectas de cultivares de Puebla-Veracruz, en contraste con seis sitios de poblaciones silvestres de Oaxaca. La variación aromática en cultivares esta determinada por seis grupos que corresponden a cinco grupos genotípicos, los cuales han disminuido la concentración de los tres compuestos menores (CM): ácido p-hidroxibenzóico, ácido vaníllico e p-hidroxibenzaldehído, en relación al contenido de vainillina. La variación aromática en poblaciones silvestres está definida por cuatro grupos, donde el aroma responde a la existencia de mayor concentración de compuestos menores en relación a vainillina
Yukiko Negishi - One of the best experts on this subject based on the ideXlab platform.
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BIOSYNTHESIS OF VANILLIN VIA FERULIC ACID IN Vanilla Planifolia
2020Co-Authors: Osamu Negishi, Yukiko NegishiAbstract:Abstract Several 14 C-labelled precursors were fed to Vanilla bean disks and their conversions to glucovanillin examined. The results showed radioactivities of 11%, 15%, 29% and 24% were incorporated into glucovanillin within 24 hr from 14 C-labelled phenylalanine, 4-coumaric acid, ferulic acid and methionine, respectively. In the process of incorporation of methionine into glucovanillin, 14 CH 3 of methionine was also trapped by unlabelled ferulic acid. However, 14 C-labelled 4-hydroxybenzaldehyde and 4-hydroxybenzyl alcohol were not converted to glucovanillin. These results suggest that the synthetic pathway in Vanilla Planifolia for vanillin is 4-coumaric acid →→ ferulic acid → vanillin → glucovanillin
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Phenylpropanoid 2,3-dioxygenase involved in the cleavage of the ferulic acid side chain to form vanillin and glyoxylic acid in Vanilla Planifolia.
Bioscience biotechnology and biochemistry, 2017Co-Authors: Osamu Negishi, Yukiko NegishiAbstract:Enzyme catalyzing the cleavage of the phenylpropanoid side chain was partially purified by ion exchange and gel filtration column chromatography after (NH4)2SO4 precipitation. Enzyme activities were dependent on the concentration of dithiothreitol (DTT) or glutathione (GSH) and activated by addition of 0.5 mM Fe2+. Enzyme activity for ferulic acid was as high as for 4-coumaric acid in the presence of GSH, suggesting that GSH acts as an endogenous reductant in vanillin biosynthesis. Analyses of the enzymatic reaction products with quantitative NMR (qNMR) indicated that an amount of glyoxylic acid (GA) proportional to vanillin was released from ferulic acid by the enzymatic reaction. These results suggest that phenylpropanoid 2,3-dioxygenase is involved in the cleavage of the ferulic acid side chain to form vanillin and GA in Vanilla Planifolia.
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Phenylpropanoid 2,3-dioxygenase involved in the cleavage of the ferulic acid side chain to form vanillin and glyoxylic acid in Vanilla Planifolia
2017Co-Authors: Osamu Negishi, Yukiko NegishiAbstract:Enzyme catalyzing the cleavage of the phenylpropanoid side chain was partially purified by ion exchange and gel filtration column chromatography after (NH4)2SO4 precipitation. Enzyme activities were dependent on the concentration of dithiothreitol (DTT) or glutathione (GSH) and activated by addition of 0.5 mM Fe2+. Enzyme activity for ferulic acid was as high as for 4-coumaric acid in the presence of GSH, suggesting that GSH acts as an endogenous reductant in vanillin biosynthesis. Analyses of the enzymatic reaction products with quantitative NMR (qNMR) indicated that an amount of glyoxylic acid (GA) proportional to vanillin was released from ferulic acid by the enzymatic reaction. These results suggest that phenylpropanoid 2,3-dioxygenase is involved in the cleavage of the ferulic acid side chain to form vanillin and GA in Vanilla Planifolia. Phenylpropanoid 2,3-dioxygenase is involved in the cleavage of the ferulic acid side chain to form vanillin and glyoxylic acid in Vanilla Planifolia.
Fang Chen - One of the best experts on this subject based on the ideXlab platform.
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A re-evaluation of the final step of vanillin biosynthesis in the orchid Vanilla Planifolia.
Phytochemistry, 2017Co-Authors: Hailian Yang, Andrzej Podstolski, Galina Kourteva, Fang Chen, Jaime Barros-rios, Xiaolan Rao, Hui Shen, Chenggang Liu, Faith C. Belanger, Daphna Havkin-frenkelAbstract:Abstract A recent publication describes an enzyme from the Vanilla orchid Vanilla Planifolia with the ability to convert ferulic acid directly to vanillin. The authors propose that this represents the final step in the biosynthesis of vanillin, which is then converted to its storage form, glucovanillin, by glycosylation. The existence of such a “vanillin synthase” could enable biotechnological production of vanillin from ferulic acid using a “natural” Vanilla enzyme. The proposed vanillin synthase exhibits high identity to cysteine proteases, and is identical at the protein sequence level to a protein identified in 2003 as being associated with the conversion of 4-coumaric acid to 4-hydroxybenzaldehyde. We here demonstrate that the recombinant cysteine protease-like protein, whether expressed in an in vitro transcription-translation system, E. coli , yeast, or plants, is unable to convert ferulic acid to vanillin. Rather, the protein is a component of an enzyme complex that preferentially converts 4-coumaric acid to 4-hydroxybenzaldehyde, as demonstrated by the purification of this complex and peptide sequencing. Furthermore, RNA sequencing provides evidence that this protein is expressed in many tissues of V. Planifolia irrespective of whether or not they produce vanillin. On the basis of our results, V. Planifolia does not appear to contain a cysteine protease-like “vanillin synthase” that can, by itself, directly convert ferulic acid to vanillin. The pathway to vanillin in V. Planifolia is yet to be conclusively determined.
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a deep transcriptomic analysis of pod development in the Vanilla orchid Vanilla Planifolia
BMC Genomics, 2014Co-Authors: Xiaolan Rao, Daphna Havkinfrenkel, Faith C. Belanger, Nick Krom, Yuhong Tang, Thomas Widiez, Richard A Dixon, Fang ChenAbstract:Pods of the Vanilla orchid (Vanilla Planifolia) accumulate large amounts of the flavor compound vanillin (3-methoxy, 4-hydroxy-benzaldehyde) as a glucoside during the later stages of their development. At earlier stages, the developing seeds within the pod synthesize a novel lignin polymer, catechyl (C) lignin, in their coats. Genomic resources for determining the biosynthetic routes to these compounds and other flavor components in V. Planifolia are currently limited. Using next-generation sequencing technologies, we have generated very large gene sequence datasets from Vanilla pods at different times of development, and representing different tissue types, including the seeds, hairs, placental and mesocarp tissues. This developmental series was chosen as being the most informative for interrogation of pathways of vanillin and C-lignin biosynthesis in the pod and seed, respectively. The combined 454/Illumina RNA-seq platforms provide both deep sequence coverage and high quality de novo transcriptome assembly for this non-model crop species. The annotated sequence data provide a foundation for understanding multiple aspects of the biochemistry and development of the Vanilla bean, as exemplified by the identification of candidate genes involved in lignin biosynthesis. Our transcriptome data indicate that C-lignin formation in the seed coat involves coordinate expression of monolignol biosynthetic genes with the exception of those encoding the caffeoyl coenzyme A 3-O-methyltransferase for conversion of caffeoyl to feruloyl moieties. This database provides a general resource for further studies on this important flavor species.
Galina Kourteva - One of the best experts on this subject based on the ideXlab platform.
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A re-evaluation of the final step of vanillin biosynthesis in the orchid Vanilla Planifolia.
Phytochemistry, 2017Co-Authors: Hailian Yang, Andrzej Podstolski, Galina Kourteva, Fang Chen, Jaime Barros-rios, Xiaolan Rao, Hui Shen, Chenggang Liu, Faith C. Belanger, Daphna Havkin-frenkelAbstract:Abstract A recent publication describes an enzyme from the Vanilla orchid Vanilla Planifolia with the ability to convert ferulic acid directly to vanillin. The authors propose that this represents the final step in the biosynthesis of vanillin, which is then converted to its storage form, glucovanillin, by glycosylation. The existence of such a “vanillin synthase” could enable biotechnological production of vanillin from ferulic acid using a “natural” Vanilla enzyme. The proposed vanillin synthase exhibits high identity to cysteine proteases, and is identical at the protein sequence level to a protein identified in 2003 as being associated with the conversion of 4-coumaric acid to 4-hydroxybenzaldehyde. We here demonstrate that the recombinant cysteine protease-like protein, whether expressed in an in vitro transcription-translation system, E. coli , yeast, or plants, is unable to convert ferulic acid to vanillin. Rather, the protein is a component of an enzyme complex that preferentially converts 4-coumaric acid to 4-hydroxybenzaldehyde, as demonstrated by the purification of this complex and peptide sequencing. Furthermore, RNA sequencing provides evidence that this protein is expressed in many tissues of V. Planifolia irrespective of whether or not they produce vanillin. On the basis of our results, V. Planifolia does not appear to contain a cysteine protease-like “vanillin synthase” that can, by itself, directly convert ferulic acid to vanillin. The pathway to vanillin in V. Planifolia is yet to be conclusively determined.
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unusual 4 hydroxybenzaldehyde synthase activity from tissue cultures of the Vanilla orchid Vanilla Planifolia
Phytochemistry, 2002Co-Authors: Andrzej Podstolski, Daphna Havkinfrenkel, Jacek Malinowski, Jack W. Blount, Galina KourtevaAbstract:Abstract Tissue cultures of the Vanilla orchid, Vanilla Planifolia, produce the flavor compound vanillin (4-hydroxy-3-methoxybenzaldehyde) and vanillin precursors such as 4-hydroxybenzaldehyde. A constitutively expressed enzyme activity catalyzing chain shortening of a hydroxycinnamic acid, believed to be the first reaction specific for formation of Vanilla flavor compounds, was identified in these cultures. The enzyme converts 4-coumaric acid non-oxidatively to 4-hydroxybenzaldehyde in the presence of a thiol reagent but with no co-factor requirement. Several forms of this 4-hydroxybenzaldehyde synthase (4HBS) were resolved and partially purified by a combination of hydrophobic interaction, ion exchange and gel filtration chromatography. These forms appear to be interconvertible. The unusual properties of the 4HBS, and its appearance in different protein fractions, raise questions as to its physiological role in vanillin biosynthesis in vivo.
Osamu Negishi - One of the best experts on this subject based on the ideXlab platform.
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BIOSYNTHESIS OF VANILLIN VIA FERULIC ACID IN Vanilla Planifolia
2020Co-Authors: Osamu Negishi, Yukiko NegishiAbstract:Abstract Several 14 C-labelled precursors were fed to Vanilla bean disks and their conversions to glucovanillin examined. The results showed radioactivities of 11%, 15%, 29% and 24% were incorporated into glucovanillin within 24 hr from 14 C-labelled phenylalanine, 4-coumaric acid, ferulic acid and methionine, respectively. In the process of incorporation of methionine into glucovanillin, 14 CH 3 of methionine was also trapped by unlabelled ferulic acid. However, 14 C-labelled 4-hydroxybenzaldehyde and 4-hydroxybenzyl alcohol were not converted to glucovanillin. These results suggest that the synthetic pathway in Vanilla Planifolia for vanillin is 4-coumaric acid →→ ferulic acid → vanillin → glucovanillin
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Phenylpropanoid 2,3-dioxygenase involved in the cleavage of the ferulic acid side chain to form vanillin and glyoxylic acid in Vanilla Planifolia.
Bioscience biotechnology and biochemistry, 2017Co-Authors: Osamu Negishi, Yukiko NegishiAbstract:Enzyme catalyzing the cleavage of the phenylpropanoid side chain was partially purified by ion exchange and gel filtration column chromatography after (NH4)2SO4 precipitation. Enzyme activities were dependent on the concentration of dithiothreitol (DTT) or glutathione (GSH) and activated by addition of 0.5 mM Fe2+. Enzyme activity for ferulic acid was as high as for 4-coumaric acid in the presence of GSH, suggesting that GSH acts as an endogenous reductant in vanillin biosynthesis. Analyses of the enzymatic reaction products with quantitative NMR (qNMR) indicated that an amount of glyoxylic acid (GA) proportional to vanillin was released from ferulic acid by the enzymatic reaction. These results suggest that phenylpropanoid 2,3-dioxygenase is involved in the cleavage of the ferulic acid side chain to form vanillin and GA in Vanilla Planifolia.
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Phenylpropanoid 2,3-dioxygenase involved in the cleavage of the ferulic acid side chain to form vanillin and glyoxylic acid in Vanilla Planifolia
2017Co-Authors: Osamu Negishi, Yukiko NegishiAbstract:Enzyme catalyzing the cleavage of the phenylpropanoid side chain was partially purified by ion exchange and gel filtration column chromatography after (NH4)2SO4 precipitation. Enzyme activities were dependent on the concentration of dithiothreitol (DTT) or glutathione (GSH) and activated by addition of 0.5 mM Fe2+. Enzyme activity for ferulic acid was as high as for 4-coumaric acid in the presence of GSH, suggesting that GSH acts as an endogenous reductant in vanillin biosynthesis. Analyses of the enzymatic reaction products with quantitative NMR (qNMR) indicated that an amount of glyoxylic acid (GA) proportional to vanillin was released from ferulic acid by the enzymatic reaction. These results suggest that phenylpropanoid 2,3-dioxygenase is involved in the cleavage of the ferulic acid side chain to form vanillin and GA in Vanilla Planifolia. Phenylpropanoid 2,3-dioxygenase is involved in the cleavage of the ferulic acid side chain to form vanillin and glyoxylic acid in Vanilla Planifolia.
