The Experts below are selected from a list of 243 Experts worldwide ranked by ideXlab platform
Yukui Zhang - One of the best experts on this subject based on the ideXlab platform.
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quantitative proteomics analysis of deer antlerogenic periosteal cells reveals potential bioactive factors in Velvet Antlers
Journal of Chromatography A, 2020Co-Authors: Zhigang Sui, Yukui Zhang, Hongmei Sun, Yejing Weng, Xiaodan Zhang, Mingwei Sun, Rui Sun, Baofeng Zhao, Zhen Liang, Lihua ZhangAbstract:Abstract Velvet Antlers (VA) have been used as medicines and nutraceuticals for over 2000 years. Meanwhile, deer Antlers are the only mammalian organs that can fully regenerate after annual shedding. The antler formation and regeneration rely on the stem cells resident in antlerogenic periosteum (AP), transplantation of which can induce ectopic antler formation. Here, a comprehensive quantitative proteomic analysis of antlerogenic periosteal cells (AP cells), compared with the adjacent facial periosteal cells (FP cells), was carried out, from both extracellular and intracellular perspectives. In this study, the stable isotope labeling by amino acids in cell culture (SILAC) was applied to ensure the precision of quantification. Then, the protein equalization strategy and reverse-phase liquid chromatography (RPLC) separation in high pH were utilized to improve the depth of proteome profiling. Proteomics analysis of the conditioned media (CM) from AP and FP cells showed that significantly over-expressed extracellular proteins in AP cells were involved in cell proliferation, angiogenesis and neurogenesis. Combining the extracellular and intracellular proteomes, we found several potential secreted proteins might regulate antler formation and regeneration, such as SFRP4 and LUM. These results provide new insight into the underlying mechanism of antler formation and regeneration.
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bioactive components of Velvet Antlers and their pharmacological properties
Journal of Pharmaceutical and Biomedical Analysis, 2014Co-Authors: Lihua Zhang, Yukui ZhangAbstract:Abstract Velvet antler is one of the most important animal medicines, and has been used with a variety of functions, such as anti-fatigue, tissue repair and health promotion. In the past few years, the investigation on chemical compositions, bioactive components, and pharmacological effects has been performed, which demonstrates that Velvet Antlers could be used as an important health-promoting tonic with great nutritional and medicinal values. This review focuses on the recent advance in studying the bioactive components of Velvet Antlers.
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an activity maintaining sequential protein extraction method for bioactive assay and proteome analysis of Velvet Antlers
Talanta, 2013Co-Authors: Zhigang Sui, Lihua Zhang, Zhen Liang, Huiming Yuan, Qun Zhao, Simin Xia, Yushu Huo, Yukui ZhangAbstract:The exceptional growth rate of Velvet antler makes it a valuable model for studying the development of tissues, such as blood vessels, cartilage and bone. Meanwhile, investigating the activities of extracted proteins from Velvet Antlers promisingly leads to the discovery of new active factors which regulate the development of above-mentioned tissue types. In this study, a novel sequential protein extraction method was developed for proteome profiling and bioactivity study of Velvet Antlers. Herein, four antler growing tips were pooled to create a proportional pooled sample, and three aliquots of which were extracted in parallel using the developed extraction method. For each sample, proteins were extracted sequentially by saline solvent (0.15M sodium chloride, pH 7.0), mild acid buffer (0.15M acetate buffer, pH 4.0) and mild alkaline buffer (0.15M glycine-sodium hydroxide buffer, pH 10.0) with good bio-compatibility to prevent proteins denaturation. Then STD lysis buffer, containing 4% SDS, 0.1M Tris-HCl and 0.1M DTT, was used to extract hydrophobic proteins. The tryptic digest of each fraction was analyzed by nanoRPLC-ESI-MS/MS in triplicates, with false discovery rate for peptide identification adjusted to 1% to create filtered protein group list. In total, 1423 protein groups were identified, which expanded up to 3 times of the previous published dataset. The relative standard deviation of identified peptide and protein group number for all analyses indicated the good reproducibility of the developed sequential protein extraction method. Additionally, proteins extracted by acid buffer and alkaline buffer showed obvious promoting effect on the proliferation of human umbilical vein endothelial cells. All these results demonstrate that the developed sequential extraction method is efficient for the comprehensive proteome analysis and activity investigation of Velvet Antlers.
Lihua Zhang - One of the best experts on this subject based on the ideXlab platform.
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quantitative proteomics analysis of deer antlerogenic periosteal cells reveals potential bioactive factors in Velvet Antlers
Journal of Chromatography A, 2020Co-Authors: Zhigang Sui, Yukui Zhang, Hongmei Sun, Yejing Weng, Xiaodan Zhang, Mingwei Sun, Rui Sun, Baofeng Zhao, Zhen Liang, Lihua ZhangAbstract:Abstract Velvet Antlers (VA) have been used as medicines and nutraceuticals for over 2000 years. Meanwhile, deer Antlers are the only mammalian organs that can fully regenerate after annual shedding. The antler formation and regeneration rely on the stem cells resident in antlerogenic periosteum (AP), transplantation of which can induce ectopic antler formation. Here, a comprehensive quantitative proteomic analysis of antlerogenic periosteal cells (AP cells), compared with the adjacent facial periosteal cells (FP cells), was carried out, from both extracellular and intracellular perspectives. In this study, the stable isotope labeling by amino acids in cell culture (SILAC) was applied to ensure the precision of quantification. Then, the protein equalization strategy and reverse-phase liquid chromatography (RPLC) separation in high pH were utilized to improve the depth of proteome profiling. Proteomics analysis of the conditioned media (CM) from AP and FP cells showed that significantly over-expressed extracellular proteins in AP cells were involved in cell proliferation, angiogenesis and neurogenesis. Combining the extracellular and intracellular proteomes, we found several potential secreted proteins might regulate antler formation and regeneration, such as SFRP4 and LUM. These results provide new insight into the underlying mechanism of antler formation and regeneration.
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identification of Velvet antler by random amplified polymorphism dna combined with non gel sieving capillary electrophoresis
Mitochondrial DNA, 2016Co-Authors: Guangxi Yua, Lihua ZhangAbstract:AbstractMitochondrial DNA of Velvet antler was amplified with random amplified polymorphic DNA (RAPD) technique and the PCR products were detected with non-gel sieving capillary electrophoresis to establish a RAPD-HPCE method used for identifying the authenticity of Velvet antler or it counterfeits. Factors that could affect the PCR amplification and capillary electrophoresis were optimized. Under the optimized conditions, namely, 20 mmol L−1 NaH2PO4–Na2HPO4–2 mmolL−1 EDTA buffer solution [0.8% (W/V) HPMC, 15 mmol L−1 TBAP and pH 7.3], −10 kV injection voltage and −8 kV separation voltage, Cervus nippon Temminck antler, Cervus elaphus Linnaeus antler, Rangifer tarandus antler, Cervus canadensis antler and Elaphurus davidianus antler were analyzed. The analysis on the similarity of obtained elctrophoretograms showed that there were significant differences in similarities of different Velvet Antlers, which could be used for the quick identification of the authenticity of Velvet antler samples. It can be fou...
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bioactive components of Velvet Antlers and their pharmacological properties
Journal of Pharmaceutical and Biomedical Analysis, 2014Co-Authors: Lihua Zhang, Yukui ZhangAbstract:Abstract Velvet antler is one of the most important animal medicines, and has been used with a variety of functions, such as anti-fatigue, tissue repair and health promotion. In the past few years, the investigation on chemical compositions, bioactive components, and pharmacological effects has been performed, which demonstrates that Velvet Antlers could be used as an important health-promoting tonic with great nutritional and medicinal values. This review focuses on the recent advance in studying the bioactive components of Velvet Antlers.
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an activity maintaining sequential protein extraction method for bioactive assay and proteome analysis of Velvet Antlers
Talanta, 2013Co-Authors: Zhigang Sui, Lihua Zhang, Zhen Liang, Huiming Yuan, Qun Zhao, Simin Xia, Yushu Huo, Yukui ZhangAbstract:The exceptional growth rate of Velvet antler makes it a valuable model for studying the development of tissues, such as blood vessels, cartilage and bone. Meanwhile, investigating the activities of extracted proteins from Velvet Antlers promisingly leads to the discovery of new active factors which regulate the development of above-mentioned tissue types. In this study, a novel sequential protein extraction method was developed for proteome profiling and bioactivity study of Velvet Antlers. Herein, four antler growing tips were pooled to create a proportional pooled sample, and three aliquots of which were extracted in parallel using the developed extraction method. For each sample, proteins were extracted sequentially by saline solvent (0.15M sodium chloride, pH 7.0), mild acid buffer (0.15M acetate buffer, pH 4.0) and mild alkaline buffer (0.15M glycine-sodium hydroxide buffer, pH 10.0) with good bio-compatibility to prevent proteins denaturation. Then STD lysis buffer, containing 4% SDS, 0.1M Tris-HCl and 0.1M DTT, was used to extract hydrophobic proteins. The tryptic digest of each fraction was analyzed by nanoRPLC-ESI-MS/MS in triplicates, with false discovery rate for peptide identification adjusted to 1% to create filtered protein group list. In total, 1423 protein groups were identified, which expanded up to 3 times of the previous published dataset. The relative standard deviation of identified peptide and protein group number for all analyses indicated the good reproducibility of the developed sequential protein extraction method. Additionally, proteins extracted by acid buffer and alkaline buffer showed obvious promoting effect on the proliferation of human umbilical vein endothelial cells. All these results demonstrate that the developed sequential extraction method is efficient for the comprehensive proteome analysis and activity investigation of Velvet Antlers.
Yuliasri Jamal - One of the best experts on this subject based on the ideXlab platform.
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The nutritional quality of captive sambar deer (Rusa unicolor brookei Hose, 1893) Velvet antler
2016Co-Authors: Gono Semiadi, Yuliasri JamalAbstract:time information concerning the nutrient value of Velvet antler of sambar deer (Rusa unicolor brookei Hose, 1893) is limited. Therefore, a study on the nutritional quality of Velvet antler of captive sambar deer was conducted. Velvet Antlers were obtained from captive sambar deer in Penajam Paser Utara, East Kalimantan, Indonesia, and were analyzed for its nutritional quality from the hard and soft parts. The results showed that fresh weight of a pair of Velvet antler (approx. 70 days post hard antler cast) was 523.1 g (SE = 49.99). In the soft part of the Velvet antler, ash content was 25.9 % DM (SE = 0.78) as compared to 40.4 % DM (SE = 1.07) in hard part, whilst the lipid and protein contents from the soft part were 3.3 % DM (SE = 0.20) and 70.8 % DM (SE = 2.07), respectively, higher compared to those in the hard part being 1.9 % DM (SE = 0.12) and 59.5 % DM (SE = 1.92), respectively. From the study it can be concluded that the production of Velvet antler from captive sambar deer seemed to be far from its genetic potency, and the nutritional qualities of the Velvet antler contents were not different from the red deer Cervus elaphus
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The nutritional quality of captive sambar deer (Rusa unicolor brookei Hose, 1893) Velvet antler
Biodiversitas Journal of Biological Diversity, 2015Co-Authors: Gono Semiadi, Yuliasri JamalAbstract:Semiadi G, Jamal Y. 2015. The nutritional quality of captive sambar deer (Rusa unicolor brookei Hose, 1893) Velvet antler.Biodiversitas 16: 156-160. Deer farming has been a well-developed agriculture diversification worldwide since 1970s. To the presenttime information concerning the nutrient value of Velvet antler of sambar deer (Rusa unicolor brookei Hose, 1893) is limited. Therefore,a study on the nutritional quality of Velvet antler of captive sambar deer was conducted. Velvet Antlers were obtained from captivesambar deer in Penajam Paser Utara, East Kalimantan, Indonesia, and were analyzed for its nutritional quality from the hard and softparts. The results showed that fresh weight of a pair of Velvet antler (approx. 70 days post hard antler cast) was 523.1 g (SE = 49.99). Inthe soft part of the Velvet antler, ash content was 25.9% DM (SE= 0.78) as compared to 40.4% DM (SE = 1.07) in hard part, whilst thelipid and protein contents from the soft part were 3.3% DM (SE = 0.20) and 70.8% DM (SE = 2.07), respectively, higher compared tothose in the hard part being 1.9% DM (SE = 0.12) and 59.5% DM (SE = 1.92), respectively. From the study it can be concluded that theproduction of Velvet antler from captive sambar deer seemed to be far from its genetic potency, and the nutritional qualities of the Velvetantler contents were not different from the red deer Cervus elaphus.Key words: nutritional quality, production, Rusa unicolor brookei, sambar deer, Velvet antler
Zhigang Sui - One of the best experts on this subject based on the ideXlab platform.
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quantitative proteomics analysis of deer antlerogenic periosteal cells reveals potential bioactive factors in Velvet Antlers
Journal of Chromatography A, 2020Co-Authors: Zhigang Sui, Yukui Zhang, Hongmei Sun, Yejing Weng, Xiaodan Zhang, Mingwei Sun, Rui Sun, Baofeng Zhao, Zhen Liang, Lihua ZhangAbstract:Abstract Velvet Antlers (VA) have been used as medicines and nutraceuticals for over 2000 years. Meanwhile, deer Antlers are the only mammalian organs that can fully regenerate after annual shedding. The antler formation and regeneration rely on the stem cells resident in antlerogenic periosteum (AP), transplantation of which can induce ectopic antler formation. Here, a comprehensive quantitative proteomic analysis of antlerogenic periosteal cells (AP cells), compared with the adjacent facial periosteal cells (FP cells), was carried out, from both extracellular and intracellular perspectives. In this study, the stable isotope labeling by amino acids in cell culture (SILAC) was applied to ensure the precision of quantification. Then, the protein equalization strategy and reverse-phase liquid chromatography (RPLC) separation in high pH were utilized to improve the depth of proteome profiling. Proteomics analysis of the conditioned media (CM) from AP and FP cells showed that significantly over-expressed extracellular proteins in AP cells were involved in cell proliferation, angiogenesis and neurogenesis. Combining the extracellular and intracellular proteomes, we found several potential secreted proteins might regulate antler formation and regeneration, such as SFRP4 and LUM. These results provide new insight into the underlying mechanism of antler formation and regeneration.
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an activity maintaining sequential protein extraction method for bioactive assay and proteome analysis of Velvet Antlers
Talanta, 2013Co-Authors: Zhigang Sui, Lihua Zhang, Zhen Liang, Huiming Yuan, Qun Zhao, Simin Xia, Yushu Huo, Yukui ZhangAbstract:The exceptional growth rate of Velvet antler makes it a valuable model for studying the development of tissues, such as blood vessels, cartilage and bone. Meanwhile, investigating the activities of extracted proteins from Velvet Antlers promisingly leads to the discovery of new active factors which regulate the development of above-mentioned tissue types. In this study, a novel sequential protein extraction method was developed for proteome profiling and bioactivity study of Velvet Antlers. Herein, four antler growing tips were pooled to create a proportional pooled sample, and three aliquots of which were extracted in parallel using the developed extraction method. For each sample, proteins were extracted sequentially by saline solvent (0.15M sodium chloride, pH 7.0), mild acid buffer (0.15M acetate buffer, pH 4.0) and mild alkaline buffer (0.15M glycine-sodium hydroxide buffer, pH 10.0) with good bio-compatibility to prevent proteins denaturation. Then STD lysis buffer, containing 4% SDS, 0.1M Tris-HCl and 0.1M DTT, was used to extract hydrophobic proteins. The tryptic digest of each fraction was analyzed by nanoRPLC-ESI-MS/MS in triplicates, with false discovery rate for peptide identification adjusted to 1% to create filtered protein group list. In total, 1423 protein groups were identified, which expanded up to 3 times of the previous published dataset. The relative standard deviation of identified peptide and protein group number for all analyses indicated the good reproducibility of the developed sequential protein extraction method. Additionally, proteins extracted by acid buffer and alkaline buffer showed obvious promoting effect on the proliferation of human umbilical vein endothelial cells. All these results demonstrate that the developed sequential extraction method is efficient for the comprehensive proteome analysis and activity investigation of Velvet Antlers.
J S Sim - One of the best experts on this subject based on the ideXlab platform.
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potential uses of Velvet antler as nutraceuticals functional and medical foods in the west
Journal of Nutraceuticals Functional & Medical Foods, 2000Co-Authors: Hoon H Sunwoo, J S SimAbstract:Abstract Velvet Antlers have been used as Oriental medicine for many centuries. Traditional medical reports and clinical observations from the Eastern world convincingly show that Velvet antler is biologically active. However, little information is available on chemical and biological efficacy of antler products in the West due to the incomplete understanding of the uses and pharmacological properties of Velvet Antlers. To make antler products acceptable as nutraceuticals and functional foods in the West, antler research has been conducted to isolate and characterize the chemical and biological properties of Velvet Antlers. The chemical composition of antler was determined in four sections (tip, upper, middle, and base). Contents of dry matter, collagen, ash, calcium, phosphorus, and magnesium increased (P < 0.05), and those of protein and lipid decreased (P < 0.05) downward from the tip to the base. The concentrations of uronic acid, sulfated glycosamino-glycan (GAG), and sialic acid decreased (P < 0.05)...
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Glycosaminoglycans from growing Antlers of wapiti (Cervus elaphus)
Canadian Journal of Animal Science, 1997Co-Authors: H. H. Sunwoo, L. Y. M. Sim, T. Nakano, R. J. Hudson, J S SimAbstract:The emerging wapiti industry in North America is based largely on markets for Velvet Antlers which are used in oriental medicine. Despite the economic opportunity, enthusiasm has been dampened by incomplete understanding of the chemical and pharmacological properties of Velvet antler. This study characterizes polysaccharide constituents of glycosaminoglycans in growing antler of wapiti (Cervus elaphus). Glycosaminoglycans were isolated from four sections (tip, upper, middle and base) of growing Antlers, and were studied using cellulose acetate electrophoresis, gel electrophoresis, enzymatic digestion and gel chromatography. The tip and upper sections of the antler which are rich in cartilaginous tissues contained chondroitin sulfate as a major glycosaminoglycan with small amounts of hyaluronic acid. In the middle and base sections containing bone and bone marrow, chondroitin sulfate was also a major glycosaminoglycan with small amounts of hyaluronic acid and chondroitinase-ACI resistant materials. More th...
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chemical composition of Antlers from wapiti cervus elaphus
Journal of Agricultural and Food Chemistry, 1995Co-Authors: Hoon H Sunwoo, Takuo Nakano, Robert J M Hudson, J S SimAbstract:Antlers, the fast growing structures of cervids, are fascinating tissues that are useful as a model for the study of bone growth and metabolism. They are also used for oriental medicine and tonics. However, little is known about their chemical composition. Four sets of fresh Velvet Antlers were obtained at 65 days after button casting from four 4-year-old wapiti, and the chemical composition of each antler was determined in four sections (tip, upper, middle, and base). The contents of dry matter, collagen, ash, calcium, phosphorus, and magnesium increased (p < 0.05), and those of protein and lipid decreased (p < 0.05) downward from the tip to the base. The concentrations of uronic acid, sulfated glycosaminoglycan, and sialic acid decreased (p < 0.05) downward. Amino acid and fatty acid contents, expressed as percentage of total protein and lipid, respectively, also varied (p < 0.05) among sections. The tip section, which is the growth center of the antler, had highest proportions of tyrosine and isoleucine and lowest proportions of glycine and alanine. The C18:3 omega 6 fatty acid was found in the tip section only.
