The Experts below are selected from a list of 360 Experts worldwide ranked by ideXlab platform
Ying Huang - One of the best experts on this subject based on the ideXlab platform.
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Verrucosispora sonchi sp nov a novel endophytic actinobacterium isolated from the leaves of common sowthistle sonchus oleraceus l
International Journal of Systematic and Evolutionary Microbiology, 2016Co-Authors: Shanshan Zhao, Wensheng Xiang, Ying Huang, Xiangjing WangAbstract:A novel actinobacterium, designated strain NEAU-QY3T, was isolated from the leaves of Sonchus oleraceus L. and examined using a polyphasic taxonomic approach. The organism formed single spores with smooth surface on substrate mycelia. Phylogenetic analysis based on the 16S rRNA gene sequence indicated that the strain had a close association with the genus Verrucosispora and shared the highest sequence similarity with Verrucosispora qiuiae RtIII47T (99.17 %), an association that was supported by a bootstrap value of 94 % in the neighbour-joining tree and also recovered with the maximum-likelihood algorithm. The strain also showed high 16S rRNA gene sequence similarities to Xiangella phaseoli NEAU-J5T (98.78 %), Jishengella endophytica 202201T (98.51 %), Micromonospora eburnea LK2-10T (98.28 %), Verrucosispora lutea YIM 013T (98.23 %) and Salinispora pacifica CNR-114T (98.23 %). Furthermore, phylogenetic analysis based on the gyrB gene sequences supported the conclusion that strain NEAU-QY3T should be assigned to the genus Verrucosispora . However, the DNA–DNA hybridization relatedness values between strain NEAU-QY3T and V. qiuiae RtIII47T and V. lutea YIM 013T were below 70 %. With reference to phenotypic characteristics, phylogenetic data and DNA–DNA hybridization results, strain NEAU-QY3T was readily distinguished from its most closely related strains and classified as a new species, for which the name Verrucosispora sonchi sp. nov. is proposed. The type strain is NEAU-QY3T (=CGMCC 4.7312T=DSM 101530T).
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Description of Verrucosispora qiuiae sp. nov., isolated from mangrove swamp sediment, and emended description of the genus Verrucosispora
International Journal of Systematic and Evolutionary Microbiology, 2012Co-Authors: Limin Zhang, Jisheng Ruan, Ying HuangAbstract:A Micromonospora -like strain, RtIII47T, was isolated from a mangrove swamp in Sanya, Hainan Province, China. Phylogenetic analysis based on the 16S rRNA gene sequence indicated that the strain had a close association with the genus Verrucosispora and shared the highest sequence similarity with Verrucosispora lutea YIM 013T (98.0 %). The strain also showed high 16S rRNA gene sequence similarities to Micromonospora olivasterospora DSM 43868T (97.9 %), Plantactinospora mayteni YIM 61359T (97.9 %), Salinispora tropica CNB-440T (97.8 %), Micromonospora peucetia DSM 43363T (97.7 %), Micromonospora auratinigra TT1-11T (97.7 %), Verrucosispora sediminis CGMCC 4.3550T (97.6 %) and Salinispora arenicola CNH-643T (97.5 %). Phylogenetic analysis based on the gyrB gene sequence supported the conclusion that strain RtIII47T should be assigned to the genus Verrucosispora . DNA–DNA relatedness between strain RtIII47T and the most closely related type strain, V. lutea YIM 013T, was less than 40 %. Chemotaxonomic results confirmed the taxonomic position of the isolate in the genus Verrucosispora , and revealed differences at the species level in polar lipids, whole-cell sugars and DNA G+C content. A combination of physiological and biochemical tests also distinguished this strain from other Verrucosispora species. Based on genotypic and phenotypic observations, strain RtIII47T ( = CGMCC 4.5826T = NBRC 106684T) is proposed as the type strain of a novel species, Verrucosispora qiuiae sp. nov. An emended description of the genus Verrucosispora is also provided.
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Diversity and Biosynthetic Potential of Culturable Actinomycetes Associated with Marine Sponges in the China Seas
International journal of molecular sciences, 2012Co-Authors: Jisheng Ruan, Ying HuangAbstract:The diversity and secondary metabolite potential of culturable actinomycetes associated with eight different marine sponges collected from the South China Sea and the Yellow sea were investigated. A total of 327 strains were isolated and 108 representative isolates were selected for phylogenetic analysis. Ten families and 13 genera of Actinomycetales were detected, among which five genera represent first records isolated from marine sponges. Oligotrophic medium M5 (water agar) proved to be efficient for selective isolation, and “Micromonospora–Streptomyces” was proposed as the major distribution group of sponge-associated actinomycetes from the China Seas. Ten isolates are likely to represent novel species. Sponge Hymeniacidon perleve was found to contain the highest genus diversity (seven genera) of actinomycetes. Housekeeping gene phylogenetic analyses of the isolates indicated one ubiquitous Micromonospora species, one unique Streptomyces species and one unique Verrucosispora phylogroup. Of the isolates, 27.5% displayed antimicrobial activity, and 91% contained polyketide synthase and/or nonribosomal peptide synthetase genes, indicating that these isolates had a high potential to produce secondary metabolites. The isolates from sponge Axinella sp. contained the highest presence of both antimicrobial activity and NRPS genes, while those from isolation medium DNBA showed the highest presence of antimicrobial activity and PKS I genes.
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Article Diversity and Biosynthetic Potential of Culturable Actinomycetes Associated with Marine Sponges in the China Seas
2012Co-Authors: Jisheng Ruan, Ying HuangAbstract:Abstract: The diversity and secondary metabolite potential of culturable actinomycetes associated with eight different marine sponges collected from the South China Sea and the Yellow sea were investigated. A total of 327 strains were isolated and 108 representative isolates were selected for phylogenetic analysis. Ten families and 13 genera of Actinomycetales were detected, among which five genera represent first records isolated from marine sponges. Oligotrophic medium M5 (water agar) proved to be efficient for selective isolation, and ―Micromonospora–Streptomyces ‖ was proposed as the major distribution group of sponge-associated actinomycetes from the China Seas. Ten isolates are likely to represent novel species. Sponge Hymeniacidon perleve was found to contain the highest genus diversity (seven genera) of actinomycetes. Housekeeping gene phylogenetic analyses of the isolates indicated one ubiquitous Micromonospora species, one unique Streptomyces species and one unique Verrucosispora phylogroup. Of the isolates, 27.5 % displayed antimicrobial activity, and 91 % contained polyketide synthase and/or nonribosomal peptide synthetase genes, indicating that these isolates had a high potential to produce secondary metabolites. The isolates from sponge Axinella sp
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Diversity and Biosynthetic Potential of Culturable Actinomycetes Associated with Marine Sponges in the China Seas
MDPI AG, 2012Co-Authors: Ying Huang, Jisheng RuanAbstract:The diversity and secondary metabolite potential of culturable actinomycetes associated with eight different marine sponges collected from the South China Sea and the Yellow sea were investigated. A total of 327 strains were isolated and 108 representative isolates were selected for phylogenetic analysis. Ten families and 13 genera of <em>Actinomycetales</em> were detected, among which five genera represent first records isolated from marine sponges. Oligotrophic medium M5 (water agar) proved to be efficient for selective isolation, and “<em>Micromonospora</em>–<em>Streptomyces</em>” was proposed as the major distribution group of sponge-associated actinomycetes from the China Seas. Ten isolates are likely to represent novel species. Sponge <em>Hymeniacidon</em> <em>perleve</em> was found to contain the highest genus diversity (seven genera) of actinomycetes. Housekeeping gene phylogenetic analyses of the isolates indicated one ubiquitous <em>Micromonospora</em> species, one unique<em> Streptomyces</em> species and one unique <em>Verrucosispora</em> phylogroup. Of the isolates, 27.5% displayed antimicrobial activity, and 91% contained polyketide synthase and/or nonribosomal peptide synthetase genes, indicating that these isolates had a high potential to produce secondary metabolites. The isolates from sponge <em>Axinella</em> sp. contained the highest presence of both antimicrobial activity and NRPS genes, while those from isolation medium DNBA showed the highest presence of antimicrobial activity and PKS I genes
Michael Goodfellow - One of the best experts on this subject based on the ideXlab platform.
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A novel taxonomic marker that discriminates between morphologically complex actinomycetes
Open biology, 2013Co-Authors: Geneviève Girard, Michael Goodfellow, Bjørn A. Traag, Vartul Sangal, Nadine Mascini, Paul A. Hoskisson, Gilles P. Van WezelAbstract:In the era when large whole genome bacterial datasets are generated routinely, rapid and accurate molecular systematics is becoming increasingly important. However, 16S ribosomal RNA sequencing does not always offer sufficient resolution to discriminate between closely related genera. The SsgA-like proteins are developmental regulatory proteins in sporulating actinomycetes, whereby SsgB actively recruits FtsZ during sporulation-specific cell division. Here, we present a novel method to classify actinomycetes, based on the extraordinary way the SsgA and SsgB proteins are conserved. The almost complete conservation of the SsgB amino acid (aa) sequence between members of the same genus and its high divergence between even closely related genera provides high-quality data for the classification of morphologically complex actinomycetes. Our analysis validates Kitasatospora as a sister genus to Streptomyces in the family Streptomycetaceae and suggests that Micromonospora, Salinispora and Verrucosispora may represent different clades of the same genus. It is also apparent that the aa sequence of SsgA is an accurate determinant for the ability of streptomycetes to produce submerged spores, dividing the phylogenetic tree of streptomycetes into liquid-culture sporulation and no liquid-culture sporulation branches. A new phylogenetic tree of industrially relevant actinomycetes is presented and compared with that based on 16S rRNA sequences.
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Verrucosispora fiedleri sp. nov., an actinomycete isolated from a fjord sediment which synthesizes proximicins
Antonie van Leeuwenhoek, 2012Co-Authors: Michael Goodfellow, James E M Stach, Roselyn Brown, Amanda Jones, Tiago Domingues Zucchi, Lina Ahmed, Wasu Pathom-aree, Alan T. Bull, Lixin Zhang, Jian WangAbstract:A novel filamentous actinobacterial organism, designated strain MG-37T, was isolated from a Norwegian fjord sediment and examined using a polyphasic taxonomic approach. The organism was determined to have chemotaxonomic and morphological properties consistent with its classification in the genus Verrucosispora and formed a distinct phyletic line in the Verrucosispora 16S rRNA gene tree. It was most closely related to Verrucosispora maris DSM 45365T (99.5 % 16S rRNA gene similarity) and Verrucosispora gifhornensis DSM 44337T (99.4 % 16S rRNA gene similarity) but was distinguished from these strains based on low levels of DNA:DNA relatedness (~56 and ~50 %, respectively). It was readily delineated from all of the type strains of Verrucosispora species based on a combination of phenotypic properties. Isolate MG-37T (=NCIMB 14794T = NRRL-B-24892T) should therefore be classified as the type strain of a novel species of Verrucosispora for which the name Verrucosispora fiedleri is proposed.
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Verrucosispora wenchangensis sp. nov., isolated from mangrove soil
Antonie van Leeuwenhoek, 2012Co-Authors: Qing-yi Xie, Michael Goodfellow, Roselyn Brown, Hai-peng Lin, Zixin Deng, Kui HongAbstract:An actinomycete strain 234402T was isolated from a mangrove soil sample collected in Wenchang, China. Phylogenetic analysis of the 16S rRNA gene sequence of strain 234402T indicated that the highest similarity was to Verrucosispora sediminis MS426T (99.25%). The cell wall contained meso-diaminopimelic acid. The major menaquinones were MK-9(H4) and MK-9(H6), with MK-9(H8) as minor components. The characteristic whole-cell sugars were xylose, mannose and glucose. The phospholipid profile was found to comprise phosphatidylethanolamine, diphosphatidylglycerol, phosphatidylinositol mannoside and an unknown phospholipid. The DNA G+C content was 69.2 mol%. The results of physiological and biochemical tests and low DNA–DNA relatedness demonstrated strain 234402T could be readily distinguished from the closely related Verrucosispora species. On the basis of these phenotypic and genotypic data, strain 234402T represents a novel species of the genus Verrucosispora, for which the name Verrucosispora wenchangensis sp. nov. is proposed. The type strain is 234402T (=CCTCC AA 2011018T=DSM 45674T).
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Verrucosispora maris sp nov a novel deep sea actinomycete isolated from a marine sediment which produces abyssomicins
Antonie Van Leeuwenhoek International Journal of General and Molecular Microbiology, 2012Co-Authors: Michael Goodfellow, James E M Stach, Roselyn Brown, Avinash Naga Venkata Bonda, Amanda Jones, Joanne Mexson, Hanspeter Fiedler, Tiago Domingues ZucchiAbstract:Verrucosispora isolate AB-18-032T, the abyssomicin- and proximicin-producing actinomycete, has chemotaxonomic and morphological properties consistent with its classification in the genus Verrucosispora. The organism formed a distinct phyletic line in the Verrucosispora 16S rRNA gene tree sharing similarities of 99.7%, 98.7% and 98.9% with Verrucosispora gifhornensis DSM 44337T, Verrucosispora lutea YIM 013T and Verrucosispora sediminis MS 426T, respectively. It was readily distinguished from the two latter species using a range of phenotypic features and from V. gifhornensis DSM 44337T, its nearest phylogenetic neighbor, by a DNA G+C content of 65.5 mol% obtained by thermal denaturation and fluorometry and DNA:DNA relatedness values of 64.0% and 65.0% using renaturation and fluorometric methods, respectively. It is apparent from the combined genotypic and phenotypic data that strain AB-18-032T should be classified in the genus Verrucosispora as a new species. The name Verrucosispora maris sp. nov. is proposed for this taxon with isolate AB-18-032T (= DSM 45365T = NRRL B-24793T) as the type strain.
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Genome Sequence of the Abyssomicin- and Proximicin-Producing Marine Actinomycete Verrucosispora maris AB-18-032
Journal of bacteriology, 2011Co-Authors: Hanseong Roh, Michael Goodfellow, Alan T. Bull, Gabriel C. Uguru, Su Jin Kim, Byung Yong Kim, Kyoung Heon Kim, Mervyn J. Bibb, In-geol ChoiAbstract:Verrucosispora maris AB-18-032 is a marine actinomycete that produces atrop-abyssomicin C and proximicin A, both of which have novel structures and modes of action. In order to understand the biosynthesis of these compounds, to identify further biosynthetic potential, and to facilitate rational improvement of secondary metabolite titers, we have sequenced the complete 6.7-Mb genome of Verrucosispora maris AB-18-032.
Hong Jiang - One of the best experts on this subject based on the ideXlab platform.
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Identification and antimicrobial properties of a new alkaloid produced by marine-derived Verrucosispora sp. FIM06-0036.
Natural product research, 2019Co-Authors: Chen Minghong, Ru Lin, Xie Yang, Lian Yunyang, Zhang Wenlong, Fang Dongsheng, Jia Jia, Hong JiangAbstract:The actinomycete strain FIM06-0036 was isolated from marine sponge sample collected from the East China Sea and identified as Verrucosispora sp. based upon the results of 16S rRNA sequence analysis...
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FW0622, a new siderophore isolated from marine Verrucosispora sp. by genomic mining.
Natural product research, 2019Co-Authors: Zhao Wei, Chuan-xi Wang, Ru Lin, Fei Sun, Xie Yang, Peng Fei, Fang Zhikai, Lian Yunyang, Hong JiangAbstract:Using the draft genome sequence of Verrucosispora sp. FIM060022, we have identified a new desferrioxamine-like siderophore, FW0622. This is the first chemically characterized siderophore obtained f...
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Draft Genome Sequence of Broad-Spectrum Antibiotic-Producing Marine Verrucosispora sp. Strain FIM060022.
Microbiology resource announcements, 2019Co-Authors: Wei Zhao, Ru Lin, Fei Sun, Xie Yang, Fang Zhikai, Hong JiangAbstract:ABSTRACT Verrucosispora sp. strain FIM060022 shows multiple biological activities and produces polytype structure compounds, including abyssomicins, proximicin A, lumichrome, denosine, and desferrioxamine-like compounds. We present the draft genome sequence of the strain to help predict the biosynthesis of these compounds, identify further biosynthetic potential, and facilitate directed secondary metabolite production.
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FW0622, a new siderophore isolated from marine Verrucosispora sp. by genomic mining
2019Co-Authors: Wei Zhao, Fei Peng, Chuan-xi Wang, Yang Xie, Ru Lin, Zhi-kai Fang, Fei Sun, Yun-yang Lian, Hong JiangAbstract:Using the draft genome sequence of Verrucosispora sp. FIM060022, we have identified a new desferrioxamine-like siderophore, FW0622. This is the first chemically characterized siderophore obtained from Verrucosispora. The structure was elucidated by extensive spectral analyses. The biosynthetic pathway of FW0622 was proposed to occur via the non-ribosomal peptide synthetase (NRPS)-independent (NIS) synthetase pathway based on the putative biosynthetic siderophore gene cluster in FIM060022. The results demonstrate that marine-derived Verrucosispora species deserve recognition as an important source of new natural products. Furthermore, this study verified that genome mining is an effective way to identify compounds that may be overlooked by traditional methods.
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Isolation, purification and identification of two new alkaloids metabolites from marine-derived Verrucosispora sp. FIM06025.
Natural product research, 2018Co-Authors: Chen Minghong, Ru Lin, Xie Yang, Lian Yunyang, Zhang Wenlong, Fang Dongsheng, Hong JiangAbstract:Chemical investigation of a marine-derived actinomycete strain Verrucosispora sp. FIM06025 isolated from a marine sponge sample collected from the East China Sea, resulted in the discovery of two n...
Jisheng Ruan - One of the best experts on this subject based on the ideXlab platform.
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Description of Verrucosispora qiuiae sp. nov., isolated from mangrove swamp sediment, and emended description of the genus Verrucosispora
International Journal of Systematic and Evolutionary Microbiology, 2012Co-Authors: Limin Zhang, Jisheng Ruan, Ying HuangAbstract:A Micromonospora -like strain, RtIII47T, was isolated from a mangrove swamp in Sanya, Hainan Province, China. Phylogenetic analysis based on the 16S rRNA gene sequence indicated that the strain had a close association with the genus Verrucosispora and shared the highest sequence similarity with Verrucosispora lutea YIM 013T (98.0 %). The strain also showed high 16S rRNA gene sequence similarities to Micromonospora olivasterospora DSM 43868T (97.9 %), Plantactinospora mayteni YIM 61359T (97.9 %), Salinispora tropica CNB-440T (97.8 %), Micromonospora peucetia DSM 43363T (97.7 %), Micromonospora auratinigra TT1-11T (97.7 %), Verrucosispora sediminis CGMCC 4.3550T (97.6 %) and Salinispora arenicola CNH-643T (97.5 %). Phylogenetic analysis based on the gyrB gene sequence supported the conclusion that strain RtIII47T should be assigned to the genus Verrucosispora . DNA–DNA relatedness between strain RtIII47T and the most closely related type strain, V. lutea YIM 013T, was less than 40 %. Chemotaxonomic results confirmed the taxonomic position of the isolate in the genus Verrucosispora , and revealed differences at the species level in polar lipids, whole-cell sugars and DNA G+C content. A combination of physiological and biochemical tests also distinguished this strain from other Verrucosispora species. Based on genotypic and phenotypic observations, strain RtIII47T ( = CGMCC 4.5826T = NBRC 106684T) is proposed as the type strain of a novel species, Verrucosispora qiuiae sp. nov. An emended description of the genus Verrucosispora is also provided.
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Diversity and Biosynthetic Potential of Culturable Actinomycetes Associated with Marine Sponges in the China Seas
International journal of molecular sciences, 2012Co-Authors: Jisheng Ruan, Ying HuangAbstract:The diversity and secondary metabolite potential of culturable actinomycetes associated with eight different marine sponges collected from the South China Sea and the Yellow sea were investigated. A total of 327 strains were isolated and 108 representative isolates were selected for phylogenetic analysis. Ten families and 13 genera of Actinomycetales were detected, among which five genera represent first records isolated from marine sponges. Oligotrophic medium M5 (water agar) proved to be efficient for selective isolation, and “Micromonospora–Streptomyces” was proposed as the major distribution group of sponge-associated actinomycetes from the China Seas. Ten isolates are likely to represent novel species. Sponge Hymeniacidon perleve was found to contain the highest genus diversity (seven genera) of actinomycetes. Housekeeping gene phylogenetic analyses of the isolates indicated one ubiquitous Micromonospora species, one unique Streptomyces species and one unique Verrucosispora phylogroup. Of the isolates, 27.5% displayed antimicrobial activity, and 91% contained polyketide synthase and/or nonribosomal peptide synthetase genes, indicating that these isolates had a high potential to produce secondary metabolites. The isolates from sponge Axinella sp. contained the highest presence of both antimicrobial activity and NRPS genes, while those from isolation medium DNBA showed the highest presence of antimicrobial activity and PKS I genes.
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Article Diversity and Biosynthetic Potential of Culturable Actinomycetes Associated with Marine Sponges in the China Seas
2012Co-Authors: Jisheng Ruan, Ying HuangAbstract:Abstract: The diversity and secondary metabolite potential of culturable actinomycetes associated with eight different marine sponges collected from the South China Sea and the Yellow sea were investigated. A total of 327 strains were isolated and 108 representative isolates were selected for phylogenetic analysis. Ten families and 13 genera of Actinomycetales were detected, among which five genera represent first records isolated from marine sponges. Oligotrophic medium M5 (water agar) proved to be efficient for selective isolation, and ―Micromonospora–Streptomyces ‖ was proposed as the major distribution group of sponge-associated actinomycetes from the China Seas. Ten isolates are likely to represent novel species. Sponge Hymeniacidon perleve was found to contain the highest genus diversity (seven genera) of actinomycetes. Housekeeping gene phylogenetic analyses of the isolates indicated one ubiquitous Micromonospora species, one unique Streptomyces species and one unique Verrucosispora phylogroup. Of the isolates, 27.5 % displayed antimicrobial activity, and 91 % contained polyketide synthase and/or nonribosomal peptide synthetase genes, indicating that these isolates had a high potential to produce secondary metabolites. The isolates from sponge Axinella sp
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Diversity and Biosynthetic Potential of Culturable Actinomycetes Associated with Marine Sponges in the China Seas
MDPI AG, 2012Co-Authors: Ying Huang, Jisheng RuanAbstract:The diversity and secondary metabolite potential of culturable actinomycetes associated with eight different marine sponges collected from the South China Sea and the Yellow sea were investigated. A total of 327 strains were isolated and 108 representative isolates were selected for phylogenetic analysis. Ten families and 13 genera of <em>Actinomycetales</em> were detected, among which five genera represent first records isolated from marine sponges. Oligotrophic medium M5 (water agar) proved to be efficient for selective isolation, and “<em>Micromonospora</em>–<em>Streptomyces</em>” was proposed as the major distribution group of sponge-associated actinomycetes from the China Seas. Ten isolates are likely to represent novel species. Sponge <em>Hymeniacidon</em> <em>perleve</em> was found to contain the highest genus diversity (seven genera) of actinomycetes. Housekeeping gene phylogenetic analyses of the isolates indicated one ubiquitous <em>Micromonospora</em> species, one unique<em> Streptomyces</em> species and one unique <em>Verrucosispora</em> phylogroup. Of the isolates, 27.5% displayed antimicrobial activity, and 91% contained polyketide synthase and/or nonribosomal peptide synthetase genes, indicating that these isolates had a high potential to produce secondary metabolites. The isolates from sponge <em>Axinella</em> sp. contained the highest presence of both antimicrobial activity and NRPS genes, while those from isolation medium DNBA showed the highest presence of antimicrobial activity and PKS I genes
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Description of Verrucosispora qiuiae sp. nov., isolated from mangrove swamp sediment, and emended description of the genus Verrucosispora.
International journal of systematic and evolutionary microbiology, 2011Co-Authors: Limin Zhang, Jisheng Ruan, Ying HuangAbstract:A Micromonospora-like strain, RtIII47(T), was isolated from a mangrove swamp in Sanya, Hainan Province, China. Phylogenetic analysis based on the 16S rRNA gene sequence indicated that the strain had a close association with the genus Verrucosispora and shared the highest sequence similarity with Verrucosispora lutea YIM 013(T) (98.0%). The strain also showed high 16S rRNA gene sequence similarities to Micromonospora olivasterospora DSM 43868(T) (97.9%), Plantactinospora mayteni YIM 61359(T) (97.9%), Salinispora tropica CNB-440(T) (97.8%), Micromonospora peucetia DSM 43363(T) (97.7%), Micromonospora auratinigra TT1-11(T) (97.7%), Verrucosispora sediminis CGMCC 4.3550(T) (97.6%) and Salinispora arenicola CNH-643(T) (97.5%). Phylogenetic analysis based on the gyrB gene sequence supported the conclusion that strain RtIII47(T) should be assigned to the genus Verrucosispora. DNA-DNA relatedness between strain RtIII47(T) and the most closely related type strain, V. lutea YIM 013(T), was less than 40%. Chemotaxonomic results confirmed the taxonomic position of the isolate in the genus Verrucosispora, and revealed differences at the species level in polar lipids, whole-cell sugars and DNA G+C content. A combination of physiological and biochemical tests also distinguished this strain from other Verrucosispora species. Based on genotypic and phenotypic observations, strain RtIII47(T) (=CGMCC 4.5826(T) =NBRC 106684(T)) is proposed as the type strain of a novel species, Verrucosispora qiuiae sp. nov. An emended description of the genus Verrucosispora is also provided.
Xie Yang - One of the best experts on this subject based on the ideXlab platform.
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Identification and antimicrobial properties of a new alkaloid produced by marine-derived Verrucosispora sp. FIM06-0036.
Natural product research, 2019Co-Authors: Chen Minghong, Ru Lin, Xie Yang, Lian Yunyang, Zhang Wenlong, Fang Dongsheng, Jia Jia, Hong JiangAbstract:The actinomycete strain FIM06-0036 was isolated from marine sponge sample collected from the East China Sea and identified as Verrucosispora sp. based upon the results of 16S rRNA sequence analysis...
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FW0622, a new siderophore isolated from marine Verrucosispora sp. by genomic mining.
Natural product research, 2019Co-Authors: Zhao Wei, Chuan-xi Wang, Ru Lin, Fei Sun, Xie Yang, Peng Fei, Fang Zhikai, Lian Yunyang, Hong JiangAbstract:Using the draft genome sequence of Verrucosispora sp. FIM060022, we have identified a new desferrioxamine-like siderophore, FW0622. This is the first chemically characterized siderophore obtained f...
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Draft Genome Sequence of Broad-Spectrum Antibiotic-Producing Marine Verrucosispora sp. Strain FIM060022.
Microbiology resource announcements, 2019Co-Authors: Wei Zhao, Ru Lin, Fei Sun, Xie Yang, Fang Zhikai, Hong JiangAbstract:ABSTRACT Verrucosispora sp. strain FIM060022 shows multiple biological activities and produces polytype structure compounds, including abyssomicins, proximicin A, lumichrome, denosine, and desferrioxamine-like compounds. We present the draft genome sequence of the strain to help predict the biosynthesis of these compounds, identify further biosynthetic potential, and facilitate directed secondary metabolite production.
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Isolation, purification and identification of two new alkaloids metabolites from marine-derived Verrucosispora sp. FIM06025.
Natural product research, 2018Co-Authors: Chen Minghong, Ru Lin, Xie Yang, Lian Yunyang, Zhang Wenlong, Fang Dongsheng, Hong JiangAbstract:Chemical investigation of a marine-derived actinomycete strain Verrucosispora sp. FIM06025 isolated from a marine sponge sample collected from the East China Sea, resulted in the discovery of two n...
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Compounds from marine-derived Verrucosispora sp. FIM06054 and their potential antitumour activities
Natural product research, 2014Co-Authors: Nie Yi-lei, Wu Yun-dan, Wang Chuan-xi, Xie Yang, Fang Dong-sheng, Jiang Hong, Lian Yun-yangAbstract:Strain FIM06054 was isolated from a marine sponge sample collected from the East China Sea and was characterised as a strain of Verrucosispora genus on the basis of its 16S rRNA gene sequence. One new compound, FW054-1 (1), together with a known aminofuran compound proximicin A (2), was isolated from the culture broth of Verrucosispora sp. FIM06054. Their structures were elucidated on the basis of spectral analysis. 1 and 2 showed antiproliferative activity against several human tumour cell lines.
