The Experts below are selected from a list of 5562 Experts worldwide ranked by ideXlab platform
Xin Chen - One of the best experts on this subject based on the ideXlab platform.
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Soybean Golgi SNARE 12 protein interacts with Soybean mosaic virus encoded P3N-PIPO protein.
Biochemical and biophysical research communications, 2016Co-Authors: Puwen Song, Haijian Zhi, Xiaoyan Cui, Xin ChenAbstract:Soybean mosaic virus (SMV), a member of the Potyvirus genus, is one of the most prevalent and devastating Viral pathogens in soybean-growing regions worldwide. It is generally accepted that symptom development of a Viral Plant Disease results from molecular interactions between the virus and its host Plant. P3N-PIPO, as a trans-frame protein consisting of the amino-terminal half of P3 fused to PIPO of the Potyvirus, plays a key role of Viral cell-to-cell movement. This study provides evidence that Golgi SNARE 12 (designated as GOS12) protein of soybean interacts with SMV P3N-PIPO via a two-hybrid yeast system by screening a soybean cDNA library. Bimolecular fluorescence complementation (BiFC) assay further confirmed the interaction, which occurred in the cytomembrane of Nicotiana benthamiana cells. We also confirmed that the domain involved in the interaction is PIPO domain of P3N-PIPO by two-hybrid yeast system and BiFC. It is possible that the GOS12 is an essential host factor for PD targeting of P3N-PIPO protein of potyvirus.
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soybean actin depolymerizing factor 2 interacts with soybean mosaic virus encoded p3 protein
Virus Genes, 2015Co-Authors: Lu Lu, Hexiang Luan, Guanwei Wu, Xiaoming Xu, Xin ChenAbstract:Soybean mosaic virus (SMV), a member of the Potyvirus genus, is one of the most prevalent and devastating Viral pathogens in soybean-growing regions worldwide. It is generally accepted that symptom development of a Viral Plant Disease results from molecular interactions between the virus and its host Plant. P3 protein is the most variable polyprotein in potyviruses, which potentially plays an important role in the process of the evolution of virus type specialization. However, P3 not only plays a major role in virus replication and movement, but it is also responsible for symptom development in SMV-infected Plants. This study provides evidence that actin-depolymerizing factor 2 (designated as ADF2) of soybean interacts with SMV P3 via a two-hybrid yeast system by screening a soybean cDNA library. Bimolecular fluorescence complementation assay further confirmed the interaction, which occurred in both the cytomembrane and cytoskeleton of Nicotiana benthamiana cells. The results support the hypothesis that SMV P3 might have a role in virus movement within cells.
Puwen Song - One of the best experts on this subject based on the ideXlab platform.
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Soybean Golgi SNARE 12 protein interacts with Soybean mosaic virus encoded P3N-PIPO protein.
Biochemical and biophysical research communications, 2016Co-Authors: Puwen Song, Haijian Zhi, Xiaoyan Cui, Xin ChenAbstract:Soybean mosaic virus (SMV), a member of the Potyvirus genus, is one of the most prevalent and devastating Viral pathogens in soybean-growing regions worldwide. It is generally accepted that symptom development of a Viral Plant Disease results from molecular interactions between the virus and its host Plant. P3N-PIPO, as a trans-frame protein consisting of the amino-terminal half of P3 fused to PIPO of the Potyvirus, plays a key role of Viral cell-to-cell movement. This study provides evidence that Golgi SNARE 12 (designated as GOS12) protein of soybean interacts with SMV P3N-PIPO via a two-hybrid yeast system by screening a soybean cDNA library. Bimolecular fluorescence complementation (BiFC) assay further confirmed the interaction, which occurred in the cytomembrane of Nicotiana benthamiana cells. We also confirmed that the domain involved in the interaction is PIPO domain of P3N-PIPO by two-hybrid yeast system and BiFC. It is possible that the GOS12 is an essential host factor for PD targeting of P3N-PIPO protein of potyvirus.
Haijian Zhi - One of the best experts on this subject based on the ideXlab platform.
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Soybean Golgi SNARE 12 protein interacts with Soybean mosaic virus encoded P3N-PIPO protein.
Biochemical and biophysical research communications, 2016Co-Authors: Puwen Song, Haijian Zhi, Xiaoyan Cui, Xin ChenAbstract:Soybean mosaic virus (SMV), a member of the Potyvirus genus, is one of the most prevalent and devastating Viral pathogens in soybean-growing regions worldwide. It is generally accepted that symptom development of a Viral Plant Disease results from molecular interactions between the virus and its host Plant. P3N-PIPO, as a trans-frame protein consisting of the amino-terminal half of P3 fused to PIPO of the Potyvirus, plays a key role of Viral cell-to-cell movement. This study provides evidence that Golgi SNARE 12 (designated as GOS12) protein of soybean interacts with SMV P3N-PIPO via a two-hybrid yeast system by screening a soybean cDNA library. Bimolecular fluorescence complementation (BiFC) assay further confirmed the interaction, which occurred in the cytomembrane of Nicotiana benthamiana cells. We also confirmed that the domain involved in the interaction is PIPO domain of P3N-PIPO by two-hybrid yeast system and BiFC. It is possible that the GOS12 is an essential host factor for PD targeting of P3N-PIPO protein of potyvirus.
Xiaoyan Cui - One of the best experts on this subject based on the ideXlab platform.
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Soybean Golgi SNARE 12 protein interacts with Soybean mosaic virus encoded P3N-PIPO protein.
Biochemical and biophysical research communications, 2016Co-Authors: Puwen Song, Haijian Zhi, Xiaoyan Cui, Xin ChenAbstract:Soybean mosaic virus (SMV), a member of the Potyvirus genus, is one of the most prevalent and devastating Viral pathogens in soybean-growing regions worldwide. It is generally accepted that symptom development of a Viral Plant Disease results from molecular interactions between the virus and its host Plant. P3N-PIPO, as a trans-frame protein consisting of the amino-terminal half of P3 fused to PIPO of the Potyvirus, plays a key role of Viral cell-to-cell movement. This study provides evidence that Golgi SNARE 12 (designated as GOS12) protein of soybean interacts with SMV P3N-PIPO via a two-hybrid yeast system by screening a soybean cDNA library. Bimolecular fluorescence complementation (BiFC) assay further confirmed the interaction, which occurred in the cytomembrane of Nicotiana benthamiana cells. We also confirmed that the domain involved in the interaction is PIPO domain of P3N-PIPO by two-hybrid yeast system and BiFC. It is possible that the GOS12 is an essential host factor for PD targeting of P3N-PIPO protein of potyvirus.
Changyong Zhou - One of the best experts on this subject based on the ideXlab platform.
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In vitro binding and bimolecular fluorescence complementation assays suggest an interaction between tomato mosaic virus coat protein and tobacco chloroplast ferredoxin I.
Archives of virology, 2013Co-Authors: Xianchao Sun, Mengdie Shi, Ning Zhang, Ling Qing, Changyong ZhouAbstract:Symptom development of a Viral Plant Disease results from molecular interactions between the virus and its host Plant. Tomato mosaic virus coat protein (ToMV CP) not only plays a major role in virion assembly and long-distance movement but is also responsible for symptom development in ToMV-infected Plants. This study provides evidence that chloroplast ferredoxin I (Fd I) interacts with ToMV CP in a GAL4-based two-hybrid yeast system for screening a Nicotiana tabacum cDNA library. The interaction between CP and Fd I was confirmed by in vitro binding and bimolecular fluorescence complementation assays in Plant cells.
