The Experts below are selected from a list of 276 Experts worldwide ranked by ideXlab platform
P. Marinho - One of the best experts on this subject based on the ideXlab platform.
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Design of specie-specific primers for Virus Diagnosis in plants with PCR
Proceedings. Fourth IEEE Symposium on Bioinformatics and Bioengineering, 2004Co-Authors: K. Rocha, C. Medeiros, M. Monteiro, L. Goncalves, P. MarinhoAbstract:We propose a specialist software to diagnose viral disease in plants. Our strategy is to align nucleotide sequences of plant Virus to discover specie-specific regions of genes of the viral genomes, so as to design a primer. The program designs oligo-nucleotide primers used for polymerase chain reaction (PCR), a very cheap Diagnosis technique. The user can specify (or use default) constraints for primer and amplified product lengths, as percentage of G+C, absolute or relative melting temperatures, and primer 3' nucleotides. The program screens candidate primer sequences with displayed user-specifiable parameters in order to help minimizing nonspecific priming and primer secondary structure. We tested this tool by designing two specific primers which were used to amplify known viral species, then used to perform a Virus Diagnosis.
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BIBE - Design of specie-specific primers for Virus Diagnosis in plants with PCR
Proceedings. Fourth IEEE Symposium on Bioinformatics and Bioengineering, 2004Co-Authors: K. Rocha, C. Medeiros, M. Monteiro, L. Goncalves, P. MarinhoAbstract:We propose a specialist software to diagnose viral disease in plants. Our strategy is to align nucleotide sequences of plant Virus to discover specie-specific regions of genes of the viral genomes, so as to design a primer. The program designs oligo-nucleotide primers used for polymerase chain reaction (PCR), a very cheap Diagnosis technique. The user can specify (or use default) constraints for primer and amplified product lengths, as percentage of G+C, absolute or relative melting temperatures, and primer 3' nucleotides. The program screens candidate primer sequences with displayed user-specifiable parameters in order to help minimizing nonspecific priming and primer secondary structure. We tested this tool by designing two specific primers which were used to amplify known viral species, then used to perform a Virus Diagnosis.
Liliane Grangeot-keros - One of the best experts on this subject based on the ideXlab platform.
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Congenital HCMV infection: A collaborative and comparative study of Virus detection in amniotic fluid by culture and by PCR
Journal of Clinical Virology, 2001Co-Authors: Stephanie Gouarin, P. Palmer, D. Cointe, S. Rogez, François Freymuth, F. Denis, Flore Rozenberg, Astrid Vabret, Pierre Lebon, Liliane Grangeot-kerosAbstract:CytomegaloVirus (HCMV) infection is the leading cause of congenital Virus infection in developed countries, affecting an estimated 1% of births. This antenatal infection can cause serious sequelae. Strategies for prevention and treatment must, therefore, be agreed upon, entailing a preliminary performance assessment of antenatal Virus Diagnosis techniques. Between 1992 and 1999, HCMV serology status was established for 19 456 pregnant women in four French hospitals. Seronegative patients (55.4%) were given serology screening, and antenatal Diagnosis was given to 152 women who had shown seroconversion during their pregnancies (1.4%). The detection of HCMV transmission from mother to fetus was finally established in 95 cases, using polymerase chain reaction (PCR) and viral culture methods for detecting HCMV in the amniotic fluid. These results were compared with viral culture of children's urine after birth, enabling us to distinguish between children really infected in utero (30%) and non-infected children (70%). The results of the Virus culture and those of PCR were identical in 94 of the 95 cases, with one discrepancy (culture-/PCR+). The two Diagnosis techniques had identical sensitivity (72%), with culture proving slightly more specific than PCR (98.4% as opposed to 96.9%). Positive prediction values for culture and for PCR were, respectively, 95.6 and 91.3%. Antenatal Virus Diagnosis on amniotic fluid was negative with both techniques in 8 out of 29 cases of children born with HCMV infection (VPN=89%). Over half of these wrongly negative results can be explained by amniocentesis carried out too early in the pregnancy or too early with respect to the mother's primary infection. Copyright © 2001 Elsevier Science B.V.
Gonzalo Bearman - One of the best experts on this subject based on the ideXlab platform.
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Bacterial Pyomyositis Associated with Human Immunodeficiency Virus: Diagnosis, Management, and Review of the Recent Literature
Current Treatment Options in Infectious Diseases, 2019Co-Authors: Laura Pedersen, Kathryn Hess, Sangeeta Sastry, Gonzalo BearmanAbstract:Purpose of review We present a case and provide a review of all the confirmed cases of pyomyositis in human immunodeficiency Virus (HIV)-positive patients in the literature after the last published case series, from September 16, 2004 to February 1, 2019. The goal of this review is to examine recent trends in causative organisms, diagnostic modalities, and duration of therapy for patients with HIV and pyomyositis in the antiretroviral therapy (ART) era. Recent findings Pyomyositis affects predominately the lower extremities and can have extra-muscular manifestations. Magnetic resonance imaging is the gold standard for Diagnosis but computed topography may be sufficient. Without appropriate clinical suspicion, pyomyositis can be misdiagnosed. Summary HIV remains an important risk factor to develop pyomyositis. While magnetic resonance imaging is the preferred diagnostic imaging modality, computed tomography may also be sufficient. Treatment is non-standardized and consists of surgical source control coupled with systemic intravenous and oral antibiotics of variable duration.
K. Rocha - One of the best experts on this subject based on the ideXlab platform.
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Design of specie-specific primers for Virus Diagnosis in plants with PCR
Proceedings. Fourth IEEE Symposium on Bioinformatics and Bioengineering, 2004Co-Authors: K. Rocha, C. Medeiros, M. Monteiro, L. Goncalves, P. MarinhoAbstract:We propose a specialist software to diagnose viral disease in plants. Our strategy is to align nucleotide sequences of plant Virus to discover specie-specific regions of genes of the viral genomes, so as to design a primer. The program designs oligo-nucleotide primers used for polymerase chain reaction (PCR), a very cheap Diagnosis technique. The user can specify (or use default) constraints for primer and amplified product lengths, as percentage of G+C, absolute or relative melting temperatures, and primer 3' nucleotides. The program screens candidate primer sequences with displayed user-specifiable parameters in order to help minimizing nonspecific priming and primer secondary structure. We tested this tool by designing two specific primers which were used to amplify known viral species, then used to perform a Virus Diagnosis.
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BIBE - Design of specie-specific primers for Virus Diagnosis in plants with PCR
Proceedings. Fourth IEEE Symposium on Bioinformatics and Bioengineering, 2004Co-Authors: K. Rocha, C. Medeiros, M. Monteiro, L. Goncalves, P. MarinhoAbstract:We propose a specialist software to diagnose viral disease in plants. Our strategy is to align nucleotide sequences of plant Virus to discover specie-specific regions of genes of the viral genomes, so as to design a primer. The program designs oligo-nucleotide primers used for polymerase chain reaction (PCR), a very cheap Diagnosis technique. The user can specify (or use default) constraints for primer and amplified product lengths, as percentage of G+C, absolute or relative melting temperatures, and primer 3' nucleotides. The program screens candidate primer sequences with displayed user-specifiable parameters in order to help minimizing nonspecific priming and primer secondary structure. We tested this tool by designing two specific primers which were used to amplify known viral species, then used to perform a Virus Diagnosis.
Stephanie Gouarin - One of the best experts on this subject based on the ideXlab platform.
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Congenital HCMV infection: A collaborative and comparative study of Virus detection in amniotic fluid by culture and by PCR
Journal of Clinical Virology, 2001Co-Authors: Stephanie Gouarin, P. Palmer, D. Cointe, S. Rogez, François Freymuth, F. Denis, Flore Rozenberg, Astrid Vabret, Pierre Lebon, Liliane Grangeot-kerosAbstract:CytomegaloVirus (HCMV) infection is the leading cause of congenital Virus infection in developed countries, affecting an estimated 1% of births. This antenatal infection can cause serious sequelae. Strategies for prevention and treatment must, therefore, be agreed upon, entailing a preliminary performance assessment of antenatal Virus Diagnosis techniques. Between 1992 and 1999, HCMV serology status was established for 19 456 pregnant women in four French hospitals. Seronegative patients (55.4%) were given serology screening, and antenatal Diagnosis was given to 152 women who had shown seroconversion during their pregnancies (1.4%). The detection of HCMV transmission from mother to fetus was finally established in 95 cases, using polymerase chain reaction (PCR) and viral culture methods for detecting HCMV in the amniotic fluid. These results were compared with viral culture of children's urine after birth, enabling us to distinguish between children really infected in utero (30%) and non-infected children (70%). The results of the Virus culture and those of PCR were identical in 94 of the 95 cases, with one discrepancy (culture-/PCR+). The two Diagnosis techniques had identical sensitivity (72%), with culture proving slightly more specific than PCR (98.4% as opposed to 96.9%). Positive prediction values for culture and for PCR were, respectively, 95.6 and 91.3%. Antenatal Virus Diagnosis on amniotic fluid was negative with both techniques in 8 out of 29 cases of children born with HCMV infection (VPN=89%). Over half of these wrongly negative results can be explained by amniocentesis carried out too early in the pregnancy or too early with respect to the mother's primary infection. Copyright © 2001 Elsevier Science B.V.
