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Jerry J. Reeves - One of the best experts on this subject based on the ideXlab platform.

  • Estimation of inbreeding and effective population size of full‐blood Wagyu cattle registered with the American Wagyu Cattle Association
    Journal of animal breeding and genetics = Zeitschrift fur Tierzuchtung und Zuchtungsbiologie, 2013
    Co-Authors: Erik Scraggs, Jerry J. Reeves, Ricardo Zanella, Andrzej J. Wojtowicz, Jeremy F. Taylor, C.t. Gaskins, J.m. De Avila, Holly L. Neibergs
    Abstract:

    Summary The objective of this research was to examine the population structure of full-blood (100%) Wagyu cattle registered in the United States with the American Wagyu Association, with the aim of estimating and comparing the levels of inbreeding from both pedigree and genotypic data. A total of 4132 full-blood Wagyu cattle pedigrees were assessed and used to compute the inbreeding coefficients (FIT and FST) and the effective population size (Ne) from pedigree data for the period 1994 to 2011. In addition to pedigree analysis, 47 full-blood Wagyu cattle representing eight prominent sire lines in the American Wagyu cattle population were genotyped using the Illumina BovineSNP50 BeadChip. Genotypic data were then used to estimate genomic inbreeding coefficients (FROH) by calculating runs of homozygosity. The mean inbreeding coefficient based on the pedigree data was estimated at 4.80%. The effective population size averaged 17 between the years 1994 and 2011 with an increase of 42.9 in 2000 and a drop of 1.8 in 2011. Examination of the runs of homozygosity revealed that the 47 Wagyu cattle from the eight prominent sire lines had a mean genomic inbreeding coefficient (FROH) estimated at 9.08% compared to a mean inbreeding coefficient based on pedigree data of 4.8%. These data suggest that the mean genotype inbreeding coefficient of full-blood Wagyu cattle exceeds the inbreeding coefficient identified by pedigree. Inbreeding has increased slowly at a rate of 0.03% per year over the past 17 years. Wagyu breeders should continue to utilize many sires from divergent lines and consider outcrossing to other breeds to enhance genetic diversity and minimize the adverse effects of inbreeding in Wagyu.

  • Quantitative genomics of 30 complex phenotypes in Wagyu x Angus F₁ progeny.
    International journal of biological sciences, 2012
    Co-Authors: Lifan Zhang, Jerry J. Reeves, Jan R. Busboom, Xiang Zhou, Jennifer J. Michal, Charles T. Gaskins, James V. O'fallon, Zengxiang Pan, Bo Ding, Michael V Dodson
    Abstract:

    In the present study, a total of 91 genes involved in various pathways were investigated for their associations with six carcass traits and twenty-four fatty acid composition phenotypes in a Wagyu×Angus reference population, including 43 Wagyu bulls and their potential 791 F1 progeny. Of the 182 SNPs evaluated, 102 SNPs that were in Hardy-Weinberg equilibrium with minor allele frequencies (MAF>0.15) were selected for parentage assignment and association studies with these quantitative traits. The parentage assignment revealed that 40 of 43 Wagyu sires produced over 96.71% of the calves in the population. Linkage disequilibrium analysis identified 75 of 102 SNPs derived from 54 genes as tagged SNPs. After Bonferroni correction, single-marker analysis revealed a total of 113 significant associations between 44 genes and 29 phenotypes (adjusted P

  • Evaluation of American Wagyu sires for scrotal circumference by age and body weight.
    Journal of animal science, 2002
    Co-Authors: J. M. Sosa, P. L. Senger, Jerry J. Reeves
    Abstract:

    Thirty-six percent of American Wagyu bulls do not meet the current minimum standards set by the Society of Theriogenology for the breeding soundness exam. In contrast, only 15% of bulls of domestic breeds do not meet the minimum standards. Scrotal circumference measurements of Wagyu are smaller than those of other breeds. The objective of this research was to describe scrotal circumference of Wagyu bulls as it relates to age and BW. The data set consisted of 190 Wagyu bulls housed at two locations. One hundred forty-one bulls constituted the first set of data (location 1); scrotal circumference was measured one to six times per bull aged between 13 and 70 mo. Ninety-four of the bulls underwent semen evaluation for motility and morphology. Forty-nine bulls constituted the data set for which scrotal circumference and BW was measured one to nine times per bull between 5 and 21 mo of age (location 2). Mean scrotal circumference of bulls within each age group was as follows: 12 to 14 mo, 29.8 0.2 cm (mean +/- SE); 15 to 17 mo, 31.8 +/- 0.2 cm; 18 to 20 mo, 32.9 +/- 0.3 cm; 21 to 24 mo, 31.8 +/- 0.5 cm; and > 24 mo, 35.5 +/- 0.2 cm. Both age and BW were highly correlated to scrotal circumference (r = 0.81 and 0.82, respectively). Within each age group, there were a percentage of bulls that did not meet the minimum standard for scrotal circumference set by the Society of Theriogenology. The percentages were as follows: 12 to 14 mo, 46%; 15 to 17 mo, 25%; 18 to 20 mo, 33%; 21 to 24 mo, 42%; and > 24 mo, 32%. Morphology and motility were > 50% each in 91% of the bulls between ages 12 and 20 mo at location 1. Based on these data, it is recommended that Wagyu bulls be evaluated with the breed-specific minimum standards for scrotal circumference of 26 cm from 12 to 14 mo, 29 cm from 15 to 17 mo, and 30 cm from 18 to 20 mo of age.

  • Differences among Wagyu sires for USDA carcass traits and palatability attributes of cooked ribeye steaks.
    Journal of animal science, 2000
    Co-Authors: J. A. Elias Calles, Jerry J. Reeves, Jan R. Busboom, Charles T. Gaskins, J.d. Cronrath, Susan K. Duckett, Ray W. Wright
    Abstract:

    The objective of this experiment was to evaluate the effects of various Wagyu sires on carcass quality traits and palatability attributes of cooked rib- eye steaks. Wagyu sires were used and grouped as Old (n = 6) or New (n = 2) sires, based on the chronological order in which they were imported into the United States. One hundred thirteen F1 heifer and steer calves sired by Wagyu bulls out of three different sources of cows were fed a backgrounding diet for 112 d consisting of an 80:20 ratio of roughage:concentrate then grazed on a mixture of orchardgrass and bluegrass pasture for 84 d and finished on a 10:90 ratio of roughage:concen-

  • Sire variation in fatty acid composition of crossbred Wagyu steers and heifers.
    Meat science, 2000
    Co-Authors: J. A. Elias Calles, Jan R. Busboom, Charles T. Gaskins, J.d. Cronrath, Susan K. Duckett, Jerry J. Reeves
    Abstract:

    Effects of sires on lipid composition of subcutaneous adipose tissue and longissimus dorsi muscle were studied using 113 F(1) heifer and steer calves sired by eight Wagyu bulls out of three different cow herds. Wagyu sires were used and grouped as Old (n=6) and New (n =2) sires, respectively, based on the chronological order in which they were imported into the US. Animals were fed a backgrounding diet for 112 days consisting of an 80:20 ratio of roughage:concentrate, then grazed on orchard grass and bluegrass for 84 days, and finished on a 10:90 ratio of roughage:concentrate diet for 231 days in a feedlot. For longissimus dorsi muscle, progeny from Old sires had higher (P 0.05).

Jan R. Busboom - One of the best experts on this subject based on the ideXlab platform.

  • 274 Improved beef quality and yield in Wagyu compared to Angus sired cattle unaffected by protein supplementation during the late gestation
    Journal of Animal Science, 2019
    Co-Authors: Xiangdong Liu, Jan R. Busboom, Natasha Moffitt-hemmer, Jeanene Deavilla, Shannon Neibergs, Mark L. Nelson
    Abstract:

    Abstract Maternal nutrition during the late gestation affects marbling fat in offspring. Wagyu cattle are well known for their very high marbling with more unsaturated fatty acid contents, but they grow slower than Angus cattle. The interaction between maternal diet and breed on marbling fat development is unclear. To examine, Angus cows (621 ± 73kg) were selected and separated into two groups, bred with either Angus or Wagyu semen. During the last 90 d of gestation, cows in each group were further separated and received either a low protein diet (85% of the NRC metabolizable protein requirement), which mimics the protein intake common in Northwestern region when fed a wheat straw based diet, or a high protein diet (108% NRC requirement). All progeny were managed together and harvested at a final body weight of 576.5± 16.6 kg. For Wagyu sired offspring, marbling score and quality grade was higher than Angus (P < 0.01), and also had higher Ribeye area (P = 0.07). The marbling scores were higher for the low protein diet for all except Wagyu steers. No difference was detected in KPH fat and the yield grade was higher for Wagyu sired cattle than Angus (P < 0.01). In both diets, Angus offspring showed higher shear force (P < 0.05). When grouped according to sex, higher marbling was observed in heifers (P < 0.01). No difference was found in cooking yield, drip loss, but Wagyu sired offspring had thicker back fat than Angus (P < 0.01). Within the high protein diet, Wagyu beef had higher linoleic acid content compared to Angus (P < 0.05). In conclusion, Wagyu sired cattle had better quality and yield compared to Angus sired, and protein supplementation during the late gestation had no major effect on beef quality. (Supported by the USDA-NIFA grants 2015-67015-23219/2016-68006-24634 to MD)

  • 344 Protein supplementation during gestation enhances offspring growth performance of Wagyu sired but not Angus sired cattle
    Journal of Animal Science, 2019
    Co-Authors: Natasha Moffitt-hemmer, Jan R. Busboom, Xiangdong Liu, Jeanene Deavilla, Shannon Neibergs, Mark L. Nelson
    Abstract:

    Abstract Wheat straw based diets are commonly fed to pregnant cows in Pacific Northwest, which may not meet protein requirements, but its impact on growth performance of offspring is unclear. Angus is the most common breed in this region while Wagyu is gaining popularity due to enhanced meat quality. The interaction between maternal nutrition and breed on growth performance of calves was examined. Angus cross cows (621 ± 73kg) were selected and randomly allotted to two groups and inseminated with either Angus or Wagyu semen. At 185 days of pregnancy, cows in each group were further separated into two-subgroups: control diet (CON, n = 21) received 85 % of the NRC metabolizable protein requirement, and protein supplemented group (SUPP, n = 21) received 108 % of requirement until calving. Then, cows and calves were moved to pastures and the cow calf pairs grazed rangeland. After weaning, calves were backgrounded, finished, and harvested at a body weight of 576.5 ± 16.6 kg. There were no differences between CON and SUPP in birth weight. However, SUPP increased 205 days adjusted weaning weight of Wagyu progeny (P < 0.01) and average daily gain (ADG) during the period of birth to weaning (P < 0.05) compared to calves from cows fed the CON diet. Moreover, Angus progeny had higher 205 days adjusted weaning weight (P < 0.01) and ADG during birth to weaning than Wagyu fed SUPP diet (P = 0.056). In both treatments, Angus gained weight faster and showed higher weight before finishing than Wagyu (P < 0.05). Angus had higher harvest weight (P < 0.01) and maternal protein supplementation tended to increase the dressing percent (P = 0.06). In conclusion, maternal protein supplementation enhanced growth performance of Wagyu but not Angus cattle, and Angus grew faster than Wagyu sired cattle. (Supported by USDA-NIFA grants 2015-67015-23219/2016-68006-24634 to MD)

  • Quantitative genomics of 30 complex phenotypes in Wagyu x Angus F₁ progeny.
    International journal of biological sciences, 2012
    Co-Authors: Lifan Zhang, Jerry J. Reeves, Jan R. Busboom, Xiang Zhou, Jennifer J. Michal, Charles T. Gaskins, James V. O'fallon, Zengxiang Pan, Bo Ding, Michael V Dodson
    Abstract:

    In the present study, a total of 91 genes involved in various pathways were investigated for their associations with six carcass traits and twenty-four fatty acid composition phenotypes in a Wagyu×Angus reference population, including 43 Wagyu bulls and their potential 791 F1 progeny. Of the 182 SNPs evaluated, 102 SNPs that were in Hardy-Weinberg equilibrium with minor allele frequencies (MAF>0.15) were selected for parentage assignment and association studies with these quantitative traits. The parentage assignment revealed that 40 of 43 Wagyu sires produced over 96.71% of the calves in the population. Linkage disequilibrium analysis identified 75 of 102 SNPs derived from 54 genes as tagged SNPs. After Bonferroni correction, single-marker analysis revealed a total of 113 significant associations between 44 genes and 29 phenotypes (adjusted P

  • Growth, carcass characteristics, muscle conjugated linoleic acid (CLA) content, and response to intravenous glucose challenge in high percentage Wagyu, Wagyu × Limousin, and Limousin steers fed sunflower oil-containing diets12
    Journal of animal science, 2002
    Co-Authors: Priya S. Mir, Jan R. Busboom, K.a. Johnson, M.v. Dodson, Z Mir, Charles T. Gaskins, J. A. Elias Calles, P. S. Kuber, Everett L. Martin, A. J. Wood
    Abstract:

    The effect of breed and diet on insulin response to glucose challenge and its relation to intramuscular fat deposition was determined in 36 steers with 12 each of greater than 87% Wagyu (referred to as Wagyu), Wagyu x Limousin, and Limousin breeds. Weaned steers were blocked by weight into heavy, medium, and light calves and placed in six pens with two pens per weight type and with two steers of each breed per pen. Three pens with steers from each weightclass were fed backgrounding and finishing diets for 259 d, while the other three pens were fed the same diets where 6% of the barley grain was replaced with sunflower oil. Prior to initiation of the finishing phase of the study the intravenous glucose tolerance test (VGTIT) was conducted in all steers. Once steers were judged as carrying adequate 12th-rib fat, based on weight and days on feed, they were harvested and graded and samples of the longissimus muscle were procured for determination of fat content and fatty acid composition. Dietary oil improved (P = 0.011; 0.06) ADG and feed conversion efficiency of steers during the latter part of backgrounding and only ADG during early part ofthe finishing period. Generally percent kidney, pelvic, and heart fat was the only adiposity assessment increased (P = 0.003) by dietary oil. The IVGTT results indicated that insulin response to intravenous glucose was lower in Limousin steers than in Wagyu steers. Dietary oil decreased (P = 0.052) fasting plasma insulin concentration in Wagyu steers compared with Limousin steers. The correlation coefficients among the IVGTT measures and intramuscular fat content or marbling score were less than 0.4, and only a negative trend existed between fasting insulin and USDA marbling scores. However, the carcasses of the Wagyu steers graded US Choice, and 66% of the Wagyu carcasses graded US Prime, which were substantially better than the quality grades obtained for the carcasses from the other breed types. Dietary oil did not affect muscle fat content but increased (P = 0.01) conjugated linoleic acid (CLA) concentrations by 339%. Results indicated that IVGTT measures were not appropriate indices of marbling potential in cattle and that dietary oil can enhance CLA content of beef.

  • Differences among Wagyu sires for USDA carcass traits and palatability attributes of cooked ribeye steaks.
    Journal of animal science, 2000
    Co-Authors: J. A. Elias Calles, Jerry J. Reeves, Jan R. Busboom, Charles T. Gaskins, J.d. Cronrath, Susan K. Duckett, Ray W. Wright
    Abstract:

    The objective of this experiment was to evaluate the effects of various Wagyu sires on carcass quality traits and palatability attributes of cooked rib- eye steaks. Wagyu sires were used and grouped as Old (n = 6) or New (n = 2) sires, based on the chronological order in which they were imported into the United States. One hundred thirteen F1 heifer and steer calves sired by Wagyu bulls out of three different sources of cows were fed a backgrounding diet for 112 d consisting of an 80:20 ratio of roughage:concentrate then grazed on a mixture of orchardgrass and bluegrass pasture for 84 d and finished on a 10:90 ratio of roughage:concen-

Stephen B. Smith - One of the best experts on this subject based on the ideXlab platform.

  • Evidence for preadipocyte proliferation during culture of subcutaneous and intramuscular adipose tissues from Angus and Wagyu crossbred steers.
    Journal of animal science, 1994
    Co-Authors: S. G. May, David K. Lunt, J. W. Savell, J. J. Wilson, J. C. Laurenz, Stephen B. Smith
    Abstract:

    The primary objective of this study was to provide evidence for preadipocyte proliferation during culture of adipose tissue explants; a secondary objective was to compare the lipogenic activity and cellularity of adipose tissues from American Wagyu crossbred steers. Subcutaneous (s.c.) and intramuscular (i.m.) adipose tissues were obtained at slaughter from the 2nd to 6th lumbar region of the loin from Angus (n = 10) and Wagyu crossbred steers (n = 10) that had been fed for 552 d by typical Japanese production standards. Adipose tissue explants were incubated 36 h with [3H]thymidine in the absence and presence of aphidicolin (a specific inhibitor of genomic DNA replication). Adipocytes were liberated by collagenase treatment and [3H]thymidine incorporation into DNA was measured. Whereas there were no significant differences between adipose tissue depots, Wagyu s.c. and i.m. preadipocytes and stromal-vascular cells exhibited greater (P < .05) [3H]thymidine incorporation into DNA than adipocytes from Angus steers. Intramuscular adipose tissue from both breeds exhibited lower (P < .05) rates of lipogenesis from acetate both before and after long-term (36-h) incubation than s.c. adipose tissue. Furthermore, i.m. adipocytes were smaller (P < .05) than s.c. adipocytes. The activities of fatty acid synthetase and glucose-6-phosphate dehydrogenase were greater (P < .05) in Wagyu s.c. adipose tissue and less in Wagyu i.m. adipose tissue than in corresponding Angus tissues. There were no differences between breed types (P = .17) in rates of lipogenesis from acetate, either before or after explant culture.(ABSTRACT TRUNCATED AT 250 WORDS)

  • Growth and carcass characteristics of Angus and American Wagyu steers.
    Meat science, 1993
    Co-Authors: David K. Lunt, R. R. Riley, Stephen B. Smith
    Abstract:

    Ten purebred Angus and ten crossbred (3/4-7/8) American Wagyu steers were fed a corn and barley-based diet for 552 days and slaughtered. All USDA and Japanese yield and quality grade factors were evaluated and the amount of extractable lipid and moisture in totally trimmed muscle was determined. Angus steers gained 0·9 kg/head/day and American Wagyus gained 0·7 kg/head/day. This difference in growth rate resulted in Angus steers having a heavier final weight (P 0·05). Average ribeye area and kidney, pelvic and heart fat were similar for the two breeds. The calculated USDA yield grade for both breeds exceeded 6. Average USDA marbling score was nearly a degree of marbling higher for American Wagyu than for Angus, but variation within breed groups was high. Average USDA quality grade was well into USDA Prime for all carcasses and did not differ by breed (P > 0·05). Japanese yield grade factors were similar for both breeds except for cold left side weight and the yield estimation which includes an adjustment factor that favors American Wagyu. American Wagyu steers merited a higher Japanese marbling score than did Angus (P 0·05). These data clearly show that some American Wagyu steers have the genetic ability to deposit as much marbling as Japanese Black cattle raised in Japan.

  • Comparison of sensory characteristics and fatty acid composition between Wagyu crossbred and Angus steers.
    Meat science, 1993
    Co-Authors: S. G. May, David K. Lunt, C.a. Sturdivant, R.k. Miller, Stephen B. Smith
    Abstract:

    Angus (n = 10) and crossbred (34 and 78) Wagyu (n = 10) steers were fed a diet according to typical Japanese standards for 552 days. The steers were fed to gain approximately 0·90 kg/head/day. Fatty acid composition was determined for subcutaneous and intramuscular adipose tissue, and M. longissimus dorsi muscle. Trained sensory evaluation and a consumer triangle test were performed on M. longissimus dorsi muscle steaks. For subcutaneous and intramuscular tissue. Wagyu adipose tissue possessed higher (P < 0·05) percentages of 14:1, 16:1 and 18:1 and a lower (P < 0·05) percentage of 16:0 and 18:0 than corresponding tissues from Angus steers. Trained sensory panel analysis revealed no differences (P < 0·05) in any of the sensory traits between steaks from Wagyu crossbred and Angus steers. However, a consumer triangle test indicated that consumers can detect a difference between breeds.

  • Fatty acid composition of subcutaneous and intramuscular adipose tissues and M. longissimus dorsi of Wagyu cattle.
    Meat science, 1992
    Co-Authors: C.a. Sturdivant, David K. Lunt, G. C. Smith, Stephen B. Smith
    Abstract:

    Three experiments were conducted to document the fatty acid composition of tissues from purebred Wagyu cattle from Japan and North American crossbred Wagyu. In experiment 1, subcutaneous (s.c.) adipose tissues (n = 23) were obtained from Japanese cattle representing the five Japanese fat quality grades. The monounsaturated:saturated fatty acid ratio (MUFA:SFA) was greatest in fat quality grade 5 samples (2·57) and least in the fat quality grade 3 samples (2·08; P < 0·05). In experiment 2, M. longissimus dorsi and the associated intramuscular (i.m.) and s.c. adipose tissues were obtained from carcasses of Wagyu crossbred steers (1/2-7/8) raised in the USA. Fatty acid composition varied among depots, but the MUFA:SFA ratio in s.c. adipose tissue (1·46) was not different from values reported for other breeds of cattle. In experiment 3, samples of M. longissimus dorsi ribsteaks were obtained from three regions in Japan. Samples from the Gunma region had the greatest (P < 0·05) MUFA:SFA ratio (2·10), relative to samples from the Kagoshima (1·82) and Miyazaki (1·65) regions. The data indicate that beef from purebred Wagyu cattle raised in Japan is enriched in monounsaturated fatty acids, and that the degree of enrichment depends upon the region of Japan from which the samples were obtained.

I. Loxton - One of the best experts on this subject based on the ideXlab platform.

  • effect of biotin supplementation on meat quality of f1 Wagyu black angus feedlot steers of known genotype
    Meat Science, 2007
    Co-Authors: R. J. Lawrence, R. Elliott, B. W. Norton, J Doyle, I. Loxton
    Abstract:

    Biotin (D-biotin) was supplemented to F1 Wagyu/Black Angus steers fed a wheat-based ration to evaluate the effect on meat quality. One hundred and eight steers of known Wagyu sire lines were assigned to three biotin treatments (0, 10 and 20 mg/head/day) with each treatment replicated four times using an unfasted liveweight of 410.5 kg (+/- 24.42 SD). Biotin supplementation had no effect (P > 0.05) on beef marbling standard at either the 5/6th or 10/11th rib quartering site, 10/11th rib intra-muscular fat percentage, intra-muscular fat fatty acid composition or adipose melting points. Wagyu genotype had an effect (P < 0.05) on beef marbling standard and intra-muscular fat percentage at the 10/11th rib, inter-muscular and intra-muscular melting point and fatty acid composition of intra-muscular fat. A significant (P < 0.001) but poor correlation existed between beef marbling standard and intra-muscular fat percentage (R-2 = 0.198). Total conjugated linoleic acid had a highly significantly (P < 0.0001) positive correlation to intra-muscular fat percentage (R-2 = 0.446). (c) 2007 Published by Elsevier Ltd.

  • Effect of biotin supplementation on meat quality of F1 Wagyu/Black Angus feedlot steers of known genotype
    Meat science, 2007
    Co-Authors: R. J. Lawrence, J.c. Doyle, R. Elliott, B. W. Norton, I. Loxton
    Abstract:

    Biotin (D-biotin) was supplemented to F1 Wagyu/Black Angus steers fed a wheat-based ration to evaluate the effect on meat quality. One hundred and eight steers of known Wagyu sire lines were assigned to three biotin treatments (0, 10 and 20 mg/head/day) with each treatment replicated four times using an unfasted liveweight of 410.5 kg (+/- 24.42 SD). Biotin supplementation had no effect (P > 0.05) on beef marbling standard at either the 5/6th or 10/11th rib quartering site, 10/11th rib intra-muscular fat percentage, intra-muscular fat fatty acid composition or adipose melting points. Wagyu genotype had an effect (P < 0.05) on beef marbling standard and intra-muscular fat percentage at the 10/11th rib, inter-muscular and intra-muscular melting point and fatty acid composition of intra-muscular fat. A significant (P < 0.001) but poor correlation existed between beef marbling standard and intra-muscular fat percentage (R-2 = 0.198). Total conjugated linoleic acid had a highly significantly (P < 0.0001) positive correlation to intra-muscular fat percentage (R-2 = 0.446). (c) 2007 Published by Elsevier Ltd.

R. J. Lawrence - One of the best experts on this subject based on the ideXlab platform.

  • effect of biotin supplementation on meat quality of f1 Wagyu black angus feedlot steers of known genotype
    Meat Science, 2007
    Co-Authors: R. J. Lawrence, R. Elliott, B. W. Norton, J Doyle, I. Loxton
    Abstract:

    Biotin (D-biotin) was supplemented to F1 Wagyu/Black Angus steers fed a wheat-based ration to evaluate the effect on meat quality. One hundred and eight steers of known Wagyu sire lines were assigned to three biotin treatments (0, 10 and 20 mg/head/day) with each treatment replicated four times using an unfasted liveweight of 410.5 kg (+/- 24.42 SD). Biotin supplementation had no effect (P > 0.05) on beef marbling standard at either the 5/6th or 10/11th rib quartering site, 10/11th rib intra-muscular fat percentage, intra-muscular fat fatty acid composition or adipose melting points. Wagyu genotype had an effect (P < 0.05) on beef marbling standard and intra-muscular fat percentage at the 10/11th rib, inter-muscular and intra-muscular melting point and fatty acid composition of intra-muscular fat. A significant (P < 0.001) but poor correlation existed between beef marbling standard and intra-muscular fat percentage (R-2 = 0.198). Total conjugated linoleic acid had a highly significantly (P < 0.0001) positive correlation to intra-muscular fat percentage (R-2 = 0.446). (c) 2007 Published by Elsevier Ltd.

  • Effect of biotin supplementation on meat quality of F1 Wagyu/Black Angus feedlot steers of known genotype
    Meat science, 2007
    Co-Authors: R. J. Lawrence, J.c. Doyle, R. Elliott, B. W. Norton, I. Loxton
    Abstract:

    Biotin (D-biotin) was supplemented to F1 Wagyu/Black Angus steers fed a wheat-based ration to evaluate the effect on meat quality. One hundred and eight steers of known Wagyu sire lines were assigned to three biotin treatments (0, 10 and 20 mg/head/day) with each treatment replicated four times using an unfasted liveweight of 410.5 kg (+/- 24.42 SD). Biotin supplementation had no effect (P > 0.05) on beef marbling standard at either the 5/6th or 10/11th rib quartering site, 10/11th rib intra-muscular fat percentage, intra-muscular fat fatty acid composition or adipose melting points. Wagyu genotype had an effect (P < 0.05) on beef marbling standard and intra-muscular fat percentage at the 10/11th rib, inter-muscular and intra-muscular melting point and fatty acid composition of intra-muscular fat. A significant (P < 0.001) but poor correlation existed between beef marbling standard and intra-muscular fat percentage (R-2 = 0.198). Total conjugated linoleic acid had a highly significantly (P < 0.0001) positive correlation to intra-muscular fat percentage (R-2 = 0.446). (c) 2007 Published by Elsevier Ltd.

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