The Experts below are selected from a list of 13812 Experts worldwide ranked by ideXlab platform
Liyan Song - One of the best experts on this subject based on the ideXlab platform.
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leachate microbiome profile reveals bacteria archaea and eukaryote dynamics and methanogenic function during solid Waste Decomposition
Bioresource Technology, 2021Co-Authors: Shu Yang, Xuya Peng, Liyan SongAbstract:Bacterial, archaeal, and eukaryotic community composition and dynamics in leachate during solid Waste Decomposition were investigated using Illumina MiSeq sequencing. The functional enzyme-encoding genes of methanogenic pathways were also predicted via PICRUSt. Succession of bacterial, archaeal, and eukaryotic community composition in aerobic phase (AP), anaerobic acid phase (ACP), and methanogenic phase (MP) was observed. The main representatives of microbial phyla, genera, and species significantly (p < 0.05) differed at least two phases. Protist Ciliophora occurred at ACP and was prevalent in MP, suggesting a short food chain establishment in the methanogenesis. Bacterial, archaeal, fungi and eukaryotic community structure were all pH and biochemical oxygen demand (BOD5) dependent patter. Acetoclastic and hydrogenotrophic methanogenesis pathways with associated functional genes differed during solid Waste Decomposition and were inhibited in ACP.
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Leachate microbiome profile reveals bacteria, archaea and eukaryote dynamics and methanogenic function during solid Waste Decomposition
Bioresource technology, 2020Co-Authors: Shu Yang, Xuya Peng, Liyan SongAbstract:Bacterial, archaeal, and eukaryotic community composition and dynamics in leachate during solid Waste Decomposition were investigated using Illumina MiSeq sequencing. The functional enzyme-encoding genes of methanogenic pathways were also predicted via PICRUSt. Succession of bacterial, archaeal, and eukaryotic community composition in aerobic phase (AP), anaerobic acid phase (ACP), and methanogenic phase (MP) was observed. The main representatives of microbial phyla, genera, and species significantly (p
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succession of bacterial community structure and metabolic function during solid Waste Decomposition
Bioresource Technology, 2019Co-Authors: Shu Yang, Liyan SongAbstract:Bacterial community structure and metabolic function during solid Waste Decomposition were investigated using Illumina MiSeq sequencing and phylogenetic investigation of communities by reconstruction of unobserved states, respectively. Results showed that bacterial community diversity and structure differed in aerobic phase, anaerobic acid phase, and methanogenic phase. Anaerobic acid phase had significantly (p < 0.05) higher richness. Proteobacteria, Firmicutes, and Bacteroidetes were the dominant representatives with significant (p < 0.05) difference between three phases. Total nitrogen of solid Waste and pH of leachate were the important factors in shaping bacterial community composition. Bacterial metabolism, information storage and processing, and cellular processes and signaling were the primary functions during solid Waste Decomposition. Key function genes (amo, nar, nis, and nos) profiles suggested that nitrification and denitrifiation mainly occurred in AP and both AP and MP, respectively.
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Succession of bacterial community structure and metabolic function during solid Waste Decomposition.
Bioresource technology, 2019Co-Authors: Shu Yang, Liyan SongAbstract:Bacterial community structure and metabolic function during solid Waste Decomposition were investigated using Illumina MiSeq sequencing and phylogenetic investigation of communities by reconstruction of unobserved states, respectively. Results showed that bacterial community diversity and structure differed in aerobic phase, anaerobic acid phase, and methanogenic phase. Anaerobic acid phase had significantly (p
V J Harwood - One of the best experts on this subject based on the ideXlab platform.
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microbial population dynamics in laboratory scale solid Waste bioreactors in the presence or absence of biosolids
Journal of Applied Microbiology, 2009Co-Authors: Bina Nayak, Audrey D Levine, A Cardoso, V J HarwoodAbstract:Aims: Decomposition of solid Waste is microbially mediated, yet little is known about the associated structure and temporal changes in prokaryotic communities. Bioreactors were used to simulate landfill conditions and archaeal and bacterial community development in leachate was examined over 8 months. Methods and Results: Municipal solid Waste (MSW) was deposited in laboratory bioreactors with or without biosolids and combustion residues (ash). The near-neutral pH fell about half a log by day 25, but recovered to ∼7·0 by day 50. Cell concentrations in bioreactors containing only MSW were significantly higher than those from co-disposal bioreactors. Archaeal and bacterial community structure was analysed by denaturing gradient gel electrophoresis targeting 16S rRNA genes, showing temporal population shifts for both domains. mcrA sequences retrieved from a co-disposal bioreactor were predominantly affiliated with the orders Methanosarcinales and Methanomicrobiales. Conclusion: Regardless of Waste composition, microbial communities in bioreactor leachates exhibited high diversity and distinct temporal trends. The solid Waste filled bioreactors allowed simulation of solid Waste Decomposition in landfills while also reducing the variables. Significance and Impact of the Study: This study advances the basic understanding of changes in microbial community structure during solid Waste Decomposition, which may ultimately improve the efficiency of solid Waste management.
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Microbial population dynamics in laboratory‐scale solid Waste bioreactors in the presence or absence of biosolids
Journal of applied microbiology, 2009Co-Authors: Bina Nayak, Audrey D Levine, A Cardoso, V J HarwoodAbstract:Aims: Decomposition of solid Waste is microbially mediated, yet little is known about the associated structure and temporal changes in prokaryotic communities. Bioreactors were used to simulate landfill conditions and archaeal and bacterial community development in leachate was examined over 8 months. Methods and Results: Municipal solid Waste (MSW) was deposited in laboratory bioreactors with or without biosolids and combustion residues (ash). The near-neutral pH fell about half a log by day 25, but recovered to ∼7·0 by day 50. Cell concentrations in bioreactors containing only MSW were significantly higher than those from co-disposal bioreactors. Archaeal and bacterial community structure was analysed by denaturing gradient gel electrophoresis targeting 16S rRNA genes, showing temporal population shifts for both domains. mcrA sequences retrieved from a co-disposal bioreactor were predominantly affiliated with the orders Methanosarcinales and Methanomicrobiales. Conclusion: Regardless of Waste composition, microbial communities in bioreactor leachates exhibited high diversity and distinct temporal trends. The solid Waste filled bioreactors allowed simulation of solid Waste Decomposition in landfills while also reducing the variables. Significance and Impact of the Study: This study advances the basic understanding of changes in microbial community structure during solid Waste Decomposition, which may ultimately improve the efficiency of solid Waste management.
Morton A. Barlaz - One of the best experts on this subject based on the ideXlab platform.
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Systems and Methods for Studying Microbial Processes and Communities in Landfills
Advances in Environmental Microbiology, 2019Co-Authors: Joseph E. Weaver, Ling Wang, Francis L. De Los Reyesiii, Morton A. BarlazAbstract:The objective of this chapter is to review research on the microbiology of landfills. This chapter focuses on anaerobic reactions that dominate Waste Decomposition in engineered landfills and begins with a brief description of the major components of a sanitary landfill followed by a discussion of MSW (municipal solid Waste) composition. The processes by which cellulosic substrates are converted to CH4 and CO2 are described. Systems for studying landfill processes (including testing setups and sampling) are then discussed, followed by traditional and molecular methods that have been used to investigate the microbial ecology of landfills.
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Microbial ecological succession during municipal solid Waste Decomposition.
Applied microbiology and biotechnology, 2018Co-Authors: Bryan F. Staley, Francis L. De Los Reyes, Ling Wang, Morton A. BarlazAbstract:The Decomposition of landfilled refuse proceeds through distinct phases, each defined by varying environmental factors such as volatile fatty acid concentration, pH, and substrate quality. The succession of microbial communities in response to these changing conditions was monitored in a laboratory-scale simulated landfill to minimize measurement difficulties experienced at field scale. 16S rRNA gene sequences retrieved at separate stages of Decomposition showed significant succession in both Bacteria and methanogenic Archaea. A majority of Bacteria sequences in landfilled refuse belong to members of the phylum Firmicutes, while Proteobacteria levels fluctuated and Bacteroidetes levels increased as Decomposition proceeded. Roughly 44% of archaeal sequences retrieved under conditions of low pH and high acetate were strictly hydrogenotrophic (Methanomicrobiales, Methanobacteriales). Methanosarcina was present at all stages of Decomposition. Correspondence analysis showed bacterial population shifts were attributed to carboxylic acid concentration and solids hydrolysis, while archaeal populations were affected to a higher degree by pH. T-RFLP analysis showed specific taxonomic groups responded differently and exhibited unique responses during Decomposition, suggesting that species composition and abundance within Bacteria and Archaea are highly dynamic. This study shows landfill microbial demographics are highly variable across both spatial and temporal transects.
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Practice review of five bioreactor/recirculation landfills.
Waste Management, 2006Co-Authors: Craig H. Benson, D T Lane, Morton A. Barlaz, J M RaweAbstract:Five landfills were analyzed to provide a perspective of current practice and technical issues that differentiate bioreactor and recirculation landfills in North America from conventional landfills. The bioreactor and recirculation landfills were found to function in much the same manner as conventional landfills, with designs similar to established standards for Waste containment facilities. Leachate generation rates, leachate depths and temperatures, and liner temperatures were similar for landfills operated in a bioreactor/recirculation or conventional mode. Gas production data indicate accelerated Waste Decomposition from leachate recirculation at one landfill. Ambiguities in gas production data precluded a definitive conclusion that leachate recirculation accelerated Waste Decomposition at the four other landfills. Analysis of leachate quality data showed that bioreactor and recirculation landfills generally produce stronger leachate than conventional landfills during the first two to three years of recirculation. Thereafter, leachate from conventional and bioreactor landfills is similar, at least in terms of conventional indicator variables (BOD, COD, pH). While the BOD and COD decreased, the pH remained around neutral and ammonia concentrations remained elevated. Settlement data collected from two of the landfills indicate that settlements are larger and occur much faster in landfills operated as bioreactors or with leachate recirculation. The analysis also indicated that more detailed data collection over longer time periods is needed to draw definitive conclusions regarding the effects of bioreactor and recirculation operations. For each of the sites in this study, some of the analyses were limited by sparseness or ambiguity in the data sets.
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Relationship of Compressibility Parameters to Municipal Solid Waste Decomposition
Journal of Geotechnical and Geoenvironmental Engineering, 2003Co-Authors: Sahadat Hossain, Mohammed A. Gabr, Morton A. BarlazAbstract:Recently, there has been substantial interest in the enhancement of refuse Decomposition in landfills, which results in increased settlement. In this paper, changes in Waste compressibility as a function of the state of Decomposition are reported. Samples representative of residential refuse were decomposed under conditions designed to simulate Decomposition in both control and bioreactor landfills. Twenty four one-dimensional oedometer tests (63.5 mm cell) were performed on refuse prepared in laboratory-scale reactors for measurement of primary (Cc) and secondary (Ci, representing creep, and Ci, representing biological) compression indices. The state of Decomposition was quantified by the methane yield and the cellulose (C) plus hemicellulose (H) to lignin (L) ratio. The magnitude of compressibility was shown to increase as refuse decomposed and compressibility parameters were correlated with the state of Decomposition. Initial settlement increased with decreasing (C + H)/L ratio while the creep index was fairly independent of the state of Decomposition. The coefficients of primary compression (Cc) for bioreactor samples showed an increasing trend with decreasing (C + H)/L ratios. Cc increased from 0.16 to 0.36 as (C + H)/L decreased from 1.29 to 0.25, and similar values of Cc were obtained with control samples at similar (C + H)/L ratios. The creep index range was estimated at 0.020.03 for control and bioreactor samples in various states of Decomposition. The magnitude of the biological degradation index (Ci) depended on the degradation phase with the highest value of 0.19 obtained during the phase of accelerated methane production. Proposing a single Cc for landfill settlement calculations may lead to inaccurate predictions. Properties of each Waste sublayer will change as a function of the Decomposition stage, and dominating processes with appropriate compressibility parameters should be applied to individual sublayers.
Shu Yang - One of the best experts on this subject based on the ideXlab platform.
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leachate microbiome profile reveals bacteria archaea and eukaryote dynamics and methanogenic function during solid Waste Decomposition
Bioresource Technology, 2021Co-Authors: Shu Yang, Xuya Peng, Liyan SongAbstract:Bacterial, archaeal, and eukaryotic community composition and dynamics in leachate during solid Waste Decomposition were investigated using Illumina MiSeq sequencing. The functional enzyme-encoding genes of methanogenic pathways were also predicted via PICRUSt. Succession of bacterial, archaeal, and eukaryotic community composition in aerobic phase (AP), anaerobic acid phase (ACP), and methanogenic phase (MP) was observed. The main representatives of microbial phyla, genera, and species significantly (p < 0.05) differed at least two phases. Protist Ciliophora occurred at ACP and was prevalent in MP, suggesting a short food chain establishment in the methanogenesis. Bacterial, archaeal, fungi and eukaryotic community structure were all pH and biochemical oxygen demand (BOD5) dependent patter. Acetoclastic and hydrogenotrophic methanogenesis pathways with associated functional genes differed during solid Waste Decomposition and were inhibited in ACP.
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Leachate microbiome profile reveals bacteria, archaea and eukaryote dynamics and methanogenic function during solid Waste Decomposition
Bioresource technology, 2020Co-Authors: Shu Yang, Xuya Peng, Liyan SongAbstract:Bacterial, archaeal, and eukaryotic community composition and dynamics in leachate during solid Waste Decomposition were investigated using Illumina MiSeq sequencing. The functional enzyme-encoding genes of methanogenic pathways were also predicted via PICRUSt. Succession of bacterial, archaeal, and eukaryotic community composition in aerobic phase (AP), anaerobic acid phase (ACP), and methanogenic phase (MP) was observed. The main representatives of microbial phyla, genera, and species significantly (p
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succession of bacterial community structure and metabolic function during solid Waste Decomposition
Bioresource Technology, 2019Co-Authors: Shu Yang, Liyan SongAbstract:Bacterial community structure and metabolic function during solid Waste Decomposition were investigated using Illumina MiSeq sequencing and phylogenetic investigation of communities by reconstruction of unobserved states, respectively. Results showed that bacterial community diversity and structure differed in aerobic phase, anaerobic acid phase, and methanogenic phase. Anaerobic acid phase had significantly (p < 0.05) higher richness. Proteobacteria, Firmicutes, and Bacteroidetes were the dominant representatives with significant (p < 0.05) difference between three phases. Total nitrogen of solid Waste and pH of leachate were the important factors in shaping bacterial community composition. Bacterial metabolism, information storage and processing, and cellular processes and signaling were the primary functions during solid Waste Decomposition. Key function genes (amo, nar, nis, and nos) profiles suggested that nitrification and denitrifiation mainly occurred in AP and both AP and MP, respectively.
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Succession of bacterial community structure and metabolic function during solid Waste Decomposition.
Bioresource technology, 2019Co-Authors: Shu Yang, Liyan SongAbstract:Bacterial community structure and metabolic function during solid Waste Decomposition were investigated using Illumina MiSeq sequencing and phylogenetic investigation of communities by reconstruction of unobserved states, respectively. Results showed that bacterial community diversity and structure differed in aerobic phase, anaerobic acid phase, and methanogenic phase. Anaerobic acid phase had significantly (p
Bina Nayak - One of the best experts on this subject based on the ideXlab platform.
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microbial population dynamics in laboratory scale solid Waste bioreactors in the presence or absence of biosolids
Journal of Applied Microbiology, 2009Co-Authors: Bina Nayak, Audrey D Levine, A Cardoso, V J HarwoodAbstract:Aims: Decomposition of solid Waste is microbially mediated, yet little is known about the associated structure and temporal changes in prokaryotic communities. Bioreactors were used to simulate landfill conditions and archaeal and bacterial community development in leachate was examined over 8 months. Methods and Results: Municipal solid Waste (MSW) was deposited in laboratory bioreactors with or without biosolids and combustion residues (ash). The near-neutral pH fell about half a log by day 25, but recovered to ∼7·0 by day 50. Cell concentrations in bioreactors containing only MSW were significantly higher than those from co-disposal bioreactors. Archaeal and bacterial community structure was analysed by denaturing gradient gel electrophoresis targeting 16S rRNA genes, showing temporal population shifts for both domains. mcrA sequences retrieved from a co-disposal bioreactor were predominantly affiliated with the orders Methanosarcinales and Methanomicrobiales. Conclusion: Regardless of Waste composition, microbial communities in bioreactor leachates exhibited high diversity and distinct temporal trends. The solid Waste filled bioreactors allowed simulation of solid Waste Decomposition in landfills while also reducing the variables. Significance and Impact of the Study: This study advances the basic understanding of changes in microbial community structure during solid Waste Decomposition, which may ultimately improve the efficiency of solid Waste management.
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Microbial population dynamics in laboratory‐scale solid Waste bioreactors in the presence or absence of biosolids
Journal of applied microbiology, 2009Co-Authors: Bina Nayak, Audrey D Levine, A Cardoso, V J HarwoodAbstract:Aims: Decomposition of solid Waste is microbially mediated, yet little is known about the associated structure and temporal changes in prokaryotic communities. Bioreactors were used to simulate landfill conditions and archaeal and bacterial community development in leachate was examined over 8 months. Methods and Results: Municipal solid Waste (MSW) was deposited in laboratory bioreactors with or without biosolids and combustion residues (ash). The near-neutral pH fell about half a log by day 25, but recovered to ∼7·0 by day 50. Cell concentrations in bioreactors containing only MSW were significantly higher than those from co-disposal bioreactors. Archaeal and bacterial community structure was analysed by denaturing gradient gel electrophoresis targeting 16S rRNA genes, showing temporal population shifts for both domains. mcrA sequences retrieved from a co-disposal bioreactor were predominantly affiliated with the orders Methanosarcinales and Methanomicrobiales. Conclusion: Regardless of Waste composition, microbial communities in bioreactor leachates exhibited high diversity and distinct temporal trends. The solid Waste filled bioreactors allowed simulation of solid Waste Decomposition in landfills while also reducing the variables. Significance and Impact of the Study: This study advances the basic understanding of changes in microbial community structure during solid Waste Decomposition, which may ultimately improve the efficiency of solid Waste management.
