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Marc Lecuit - One of the best experts on this subject based on the ideXlab platform.
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Listeria valentina sp. nov., isolated from a water trough and the faeces of healthy sheep
International Journal of Systematic and Evolutionary Microbiology, 2020Co-Authors: Juan J Quereda, Alexandre Leclercq, Alexandra Moura, Guillaume Vales, Nathalie Tessaud-rita, Ángel Gómez-martín, Ángel García-muñoz, Pierre Thouvenot, Hélène Bracq-dieye, Marc LecuitAbstract:In the context of a study on the occurrence of Listeria species in an animal farm environment in Valencia, Spain, six Listeria-like isolates could not be assigned to any known species. Phylogenetic analysis based on the 16S rRNA gene and on 231 Listeria core genes grouped these isolates in a monophyletic clade within the genus Listeria, with highest similarity to Listeria thailandensis. Whole-genome sequence analyses based on in silico DNA-DNA hybridization, the average nucleotide blast and the pairwise amino acid identities against all currently known Listeria species confirmed that these isolates constituted a new taxon within the genus Listeria. Phenotypically, these isolates differed from other Listeria species mainly by the production of acid from inositol, the absence of acidification in presence of methyl α-d-glucoside, and the absence of α-mannosidase and nitrate reductase activities. The name Listeria valentina sp. nov. is proposed for this novel species, and the type strain is CLIP 2019/00642T (=CIP 111799T=DSM 110544T).
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Listeria thailandensis sp. nov.
International Journal of Systematic and Evolutionary Microbiology, 2019Co-Authors: Alexandre Leclercq, Alexandra Moura, Guillaume Vales, Nathalie Tessaud-rita, Christine Aguilhon, Marc LecuitAbstract:During a screening of Listeria species in food samples in Thailand, a Listeria-like bacterium was recovered from fried chicken and could not be assigned to any known species. Phylogenetic analysis based on the 16S rRNA gene and on 243 Listeria core genes placed the novel taxon within the Listeria aquatica, Listeria floridensis, Listeria fleishmannii and Listeria costaricensis clade (Listeria sensu lato), with highest similarity to L. floridensis (98.9 %) and L. costaricensis (98.8 %). Whole-genome sequence analyses based on the average nucleotide blast identity (ANI64 %) and on the percentage of conserved proteins (POCP>77 %) with currently known Listeria species confirmed that the strain constituted a new taxon within the genus Listeria. At the phenotypical level, it differs from other Listeria species by the production of acid from d-tagatose and inositol. The name Listeria thailandensis sp. nov. is proposed for the novel species, and is represented by the type strain CLIP 2015/00305T (=CIP 111635T=DSM 107638T).
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Listeria costaricensis sp. nov.
International journal of systematic and evolutionary microbiology, 2018Co-Authors: Kattia Núñez-montero, Alexandre Leclercq, Alexandra Moura, Guillaume Vales, Johnny Peraza, Javier Pizarro-cerdá, Marc LecuitAbstract:A bacterial strain isolated from a food processing drainage system in Costa Rica fulfilled the criteria as belonging to the genus Listeria , but could not be assigned to any of the known species. Phylogenetic analysis based on the 16S rRNA gene revealed highest sequence similarity with the type strain of Listeria floridensis (98.7 %). Phylogenetic analysis based on Listeria core genomes placed the novel taxon within the Listeria fleishmannii, L. floridensis and Listeria aquatica clade ( Listeria sensu lato). Whole-genome sequence analyses based on the average nucleotide blast identity (ANI 70 %) and percentage of conserved proteins (POCP>68 %) with currently known Listeria species, as well as of biochemical characterization, confirmed that the strain constituted a novel species within the genus Listeria . The name Listeria costaricensis sp. nov. is proposed for the novel species, and is represented by the type strain CLIP 2016/00682T (=CIP 111400T=DSM 105474T).
Leslie R. Goertzen - One of the best experts on this subject based on the ideXlab platform.
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Horticulture, hybrid cultivars and exotic plant invasion: a case study of Wisteria (Fabaceae)
Botanical Journal of the Linnean Society, 2008Co-Authors: Jennifer L. Trusty, Wayne C. Zipperer, B. Graeme Lockaby, Leslie R. GoertzenAbstract:Exotic Wisteria species are highly favoured for their horticultural qualities and have been cultivated in North America since the early 1800s. This study determines the identity, genetic diversity and hybrid status of 25 Asian Wisteria cultivars using plastid, mitochondrial and nuclear DNA data. Fifteen (60%) hybrid cultivars were identified. All of the ‘Wisteria sinensis’ cultivars sampled are hybrids with W. floribunda. Although W. sinensis and W. floribunda are recognized invasive species in the southeastern USA, the relationships of horticultural cultivars to naturalized plants was previously unknown. Haplotype analysis of nuclear data identifies four haplotypes shared between cultivated stock and naturalized populations in the southeastern USA. In addition, US invasive haplotypes are present in New Zealand-derived cultivars although, to date, naturalized Wisteria has not been documented in New Zealand. Finally, these data are used to make recommendations to horticulturalists of select species cultivars which may be less likely to invade US landscapes. © 2008 The Linnean Society of London, Botanical Journal of the Linnean Society, 2008, 158, 593–601.
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Identity of naturalised exotic Wisteria (Fabaceae) in the south-eastern United States
Weed Research, 2007Co-Authors: Jennifer L. Trusty, B G Lockaby, Wayne C. Zipperer, Leslie R. GoertzenAbstract:Summary Exotic Wisteria are increasingly serious invasive plants of watersheds and managed forests throughout the south-eastern United States. Naturalised individuals are frequently identified as either Wisteria floribunda or W. sinensis, but may differ significantly from the original descriptions of either of those species. Here, we use data from the nuclear and chloroplast genomes to determine the species classification or hybrid status of naturalised plants collected in five south-eastern states. Twenty-four of twenty-five collections (96%) were identified as hybrids between W. floribunda and W. sinensis. Haplotype analyses show that naturalised hybrid Wisteria is genetically diverse and that no relationship between haplotype and collection location exists. Morphological characters that clearly differentiate the introduced species cannot be used to reliably identify naturalised individuals. These data, along with observations of the continued spread of Wisteria in the south-eastern United States, suggest that hybridisation may be playing a key role in the ongoing invasion of this taxon.
Jennifer L. Trusty - One of the best experts on this subject based on the ideXlab platform.
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Horticulture, hybrid cultivars and exotic plant invasion: a case study of Wisteria (Fabaceae)
Botanical Journal of the Linnean Society, 2008Co-Authors: Jennifer L. Trusty, Wayne C. Zipperer, B. Graeme Lockaby, Leslie R. GoertzenAbstract:Exotic Wisteria species are highly favoured for their horticultural qualities and have been cultivated in North America since the early 1800s. This study determines the identity, genetic diversity and hybrid status of 25 Asian Wisteria cultivars using plastid, mitochondrial and nuclear DNA data. Fifteen (60%) hybrid cultivars were identified. All of the ‘Wisteria sinensis’ cultivars sampled are hybrids with W. floribunda. Although W. sinensis and W. floribunda are recognized invasive species in the southeastern USA, the relationships of horticultural cultivars to naturalized plants was previously unknown. Haplotype analysis of nuclear data identifies four haplotypes shared between cultivated stock and naturalized populations in the southeastern USA. In addition, US invasive haplotypes are present in New Zealand-derived cultivars although, to date, naturalized Wisteria has not been documented in New Zealand. Finally, these data are used to make recommendations to horticulturalists of select species cultivars which may be less likely to invade US landscapes. © 2008 The Linnean Society of London, Botanical Journal of the Linnean Society, 2008, 158, 593–601.
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Identity of naturalised exotic Wisteria (Fabaceae) in the south-eastern United States
Weed Research, 2007Co-Authors: Jennifer L. Trusty, B G Lockaby, Wayne C. Zipperer, Leslie R. GoertzenAbstract:Summary Exotic Wisteria are increasingly serious invasive plants of watersheds and managed forests throughout the south-eastern United States. Naturalised individuals are frequently identified as either Wisteria floribunda or W. sinensis, but may differ significantly from the original descriptions of either of those species. Here, we use data from the nuclear and chloroplast genomes to determine the species classification or hybrid status of naturalised plants collected in five south-eastern states. Twenty-four of twenty-five collections (96%) were identified as hybrids between W. floribunda and W. sinensis. Haplotype analyses show that naturalised hybrid Wisteria is genetically diverse and that no relationship between haplotype and collection location exists. Morphological characters that clearly differentiate the introduced species cannot be used to reliably identify naturalised individuals. These data, along with observations of the continued spread of Wisteria in the south-eastern United States, suggest that hybridisation may be playing a key role in the ongoing invasion of this taxon.
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Identity of naturalised exotic Wisteria (Fabaceae) in the
2007Co-Authors: Jennifer L. Trusty, B G Lockaby, W C ZipperertAbstract:. ' Summary Exotic Wisteria are increasingly serious invasive plants of watersheds and managed forests throughout the south-eastern United States. Naturalised individuals are frequently identified as either Wisteria florihunda or W. sinensis. but may differ significantly from the original descriptions of either of those species. Here, we use data from the nuclear and chloroplast genomes to determine the species classification or hybrid status of naturalised plants collected in five south-eastern states. Twenty-four of twenty-five collections (96%) were identified as hybrids between W. florihunda and W. sinensis. Raplotype analyses show that naturalised hybrid Wisteria is genetically diverse and that no relationship between haplotype and collection location exists. Morphological characters that clearly differentiate the introduced spe cies cannot be used to reliably identify naturalised individuals. These data, along with observations of the continued spread of Wisteria in the south-eastern United States, suggest that hybridisation may be playing a key role in the ongoing invasion of this taxon.
Alexandre Leclercq - One of the best experts on this subject based on the ideXlab platform.
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Listeria valentina sp. nov., isolated from a water trough and the faeces of healthy sheep
International Journal of Systematic and Evolutionary Microbiology, 2020Co-Authors: Juan J Quereda, Alexandre Leclercq, Alexandra Moura, Guillaume Vales, Nathalie Tessaud-rita, Ángel Gómez-martín, Ángel García-muñoz, Pierre Thouvenot, Hélène Bracq-dieye, Marc LecuitAbstract:In the context of a study on the occurrence of Listeria species in an animal farm environment in Valencia, Spain, six Listeria-like isolates could not be assigned to any known species. Phylogenetic analysis based on the 16S rRNA gene and on 231 Listeria core genes grouped these isolates in a monophyletic clade within the genus Listeria, with highest similarity to Listeria thailandensis. Whole-genome sequence analyses based on in silico DNA-DNA hybridization, the average nucleotide blast and the pairwise amino acid identities against all currently known Listeria species confirmed that these isolates constituted a new taxon within the genus Listeria. Phenotypically, these isolates differed from other Listeria species mainly by the production of acid from inositol, the absence of acidification in presence of methyl α-d-glucoside, and the absence of α-mannosidase and nitrate reductase activities. The name Listeria valentina sp. nov. is proposed for this novel species, and the type strain is CLIP 2019/00642T (=CIP 111799T=DSM 110544T).
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Listeria thailandensis sp. nov.
International Journal of Systematic and Evolutionary Microbiology, 2019Co-Authors: Alexandre Leclercq, Alexandra Moura, Guillaume Vales, Nathalie Tessaud-rita, Christine Aguilhon, Marc LecuitAbstract:During a screening of Listeria species in food samples in Thailand, a Listeria-like bacterium was recovered from fried chicken and could not be assigned to any known species. Phylogenetic analysis based on the 16S rRNA gene and on 243 Listeria core genes placed the novel taxon within the Listeria aquatica, Listeria floridensis, Listeria fleishmannii and Listeria costaricensis clade (Listeria sensu lato), with highest similarity to L. floridensis (98.9 %) and L. costaricensis (98.8 %). Whole-genome sequence analyses based on the average nucleotide blast identity (ANI64 %) and on the percentage of conserved proteins (POCP>77 %) with currently known Listeria species confirmed that the strain constituted a new taxon within the genus Listeria. At the phenotypical level, it differs from other Listeria species by the production of acid from d-tagatose and inositol. The name Listeria thailandensis sp. nov. is proposed for the novel species, and is represented by the type strain CLIP 2015/00305T (=CIP 111635T=DSM 107638T).
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Listeria costaricensis sp. nov.
International journal of systematic and evolutionary microbiology, 2018Co-Authors: Kattia Núñez-montero, Alexandre Leclercq, Alexandra Moura, Guillaume Vales, Johnny Peraza, Javier Pizarro-cerdá, Marc LecuitAbstract:A bacterial strain isolated from a food processing drainage system in Costa Rica fulfilled the criteria as belonging to the genus Listeria , but could not be assigned to any of the known species. Phylogenetic analysis based on the 16S rRNA gene revealed highest sequence similarity with the type strain of Listeria floridensis (98.7 %). Phylogenetic analysis based on Listeria core genomes placed the novel taxon within the Listeria fleishmannii, L. floridensis and Listeria aquatica clade ( Listeria sensu lato). Whole-genome sequence analyses based on the average nucleotide blast identity (ANI 70 %) and percentage of conserved proteins (POCP>68 %) with currently known Listeria species, as well as of biochemical characterization, confirmed that the strain constituted a novel species within the genus Listeria . The name Listeria costaricensis sp. nov. is proposed for the novel species, and is represented by the type strain CLIP 2016/00682T (=CIP 111400T=DSM 105474T).
Kazuo Yamamoto - One of the best experts on this subject based on the ideXlab platform.
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terminal n acetylgalactosamine specific leguminous lectin from Wisteria japonica as a probe for human lung squamous cell carcinoma
PLOS ONE, 2013Co-Authors: Keisuke Soga, Futaba Teruya, Hiroaki Tateno, Jun Hirabayashi, Kazuo YamamotoAbstract:Millettia japonica was recently reclassified into the genus Wisteria japonica based on chloroplast and nuclear DNA sequences. Because the seed of Wisteria floribunda expresses leguminous lectins with unique N-acetylgalactosamine-binding specificity, we purified lectin from Wisteria japonica seeds using ion exchange and gel filtration chromatography. Glycan microarray analysis demonstrated that unlike Wisteria floribunda and Wisteria brachybotrys lectins, which bind to both terminal N-acetylgalactosamine and galactose residues, Wisteria japonica lectin (WJA) specifically bound to both α- and β-linked terminal N-acetylgalactosamine, but not galactose residues on oligosaccharides and glycoproteins. Further, frontal affinity chromatography using more than 100 2-aminopyridine-labeled and p-nitrophenyl-derivatized oligosaccharides demonstrated that the ligands with the highest affinity for Wisteria japonica lectin were GalNAcβ1-3GlcNAc and GalNAcβ1-4GlcNAc, with Ka values of 9.5 × 104 and 1.4 × 105 M-1, respectively. In addition, when binding was assessed in a variety of cell lines, Wisteria japonica lectin bound specifically to EBC-1 and HEK293 cells while other Wisteria lectins bound equally to all of the cell lines tested. Wisteria japonica lectin binding to EBC-1 and HEK293 cells was dramatically decreased in the presence of N-acetylgalactosamine, but not galactose, mannose, or N-acetylglucosamine, and was completely abrogated by β-hexosaminidase-digestion of these cells. These results clearly demonstrate that Wisteria japonica lectin binds to terminal N-acetylgalactosamine but not galactose. In addition, histochemical analysis of human squamous cell carcinoma tissue sections demonstrated that Wisteria japonica lectin specifically bound to differentiated cancer tissues but not normal tissue. This novel binding characteristic of Wisteria japonica lectin has the potential to become a powerful tool for clinical applications.
