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Kathryn A Mason - One of the best experts on this subject based on the ideXlab platform.

M.e. Vergara Alcaide - One of the best experts on this subject based on the ideXlab platform.

  • ruminal fermentation and degradation patterns protozoa population and urinary purine derivatives excretion in goats and wethers fed diets based on olive leaves
    Journal of Animal Science, 2004
    Co-Authors: D Yanez R Ruiz, A Martin I Garcia, Ahmed El Moumen, M.e. Vergara Alcaide
    Abstract:

    Olives leaves, accrued during the processing of olive harvests for oil extraction, are poor in N, rich in crude fat and ADF (1.19, 8.03 and 28.2 g/100 g of DM, respectively), and relatively low in condensed tannins (11.1 mg/g of DM). Three experiments were conducted in a 2 x 3 (two animal species: goats vs. wethers; and three experimental diets: olive leaves without or with polyethylene glycol supply and olive leaves supplemented with barley and faba beans) factorial design to evaluate ruminal degradation and passage kinetics (Exp. 1), fermentation pattern and protozoa population (Exp. 2), and urinary purine derivatives excretion (Exp. 3). Polyethylene glycol was supplied to evaluate the effects of condensed tannins contained in olive leaves. Ruminal degradability of CP was low in both goats and wethers, although goats showed higher (P < 0.05) values than wethers. Supplementation of olive leaves with barley and faba beans increased (P < 0.001) ruminal degradability of DM and CP. Both goats and wethers fed olive leaves showed similarly low particulate fractional passage rates (0.021 and 0.023/h, respectively). Ingestion of olive leaves promoted low NH3-N and VFA concentrations, which reflect poor microbial activity. These concentrations, especially that of VFA, increased when barley and faba beans were added. Ingestion of olive leaves affected ruminal protozoa: Entodiniomorphida showed low concentrations and Holotricha completely disappeared. When animals received a diet based on olive leaves, barley, and faba beans, Holotricha appeared in the ruminal liquor and Entodiniomorphida increased (P < 0.001). In goats and wethers fed olive leaves alone, urinary allantoin excretion was very low (163 and 164 micromol/kg BW0.75 in goats and wethers, respectively), and moderate values (352 and 389 micromol/kg BW0.75 in goats and wethers, respectively) were observed when a diet of olive leaves, barley, and faba beans was fed. The polyethylene glycol supply did not have an effect in goats or in wethers, indicating the lack of an effect of condensed tannins in olive leaves. Ingestion of olive leaves promotes a low microbial activity, although its supplementation with readily degraded carbohydrates and protein improves microbial activity and, as a consequence, increases its ruminal degradation. In general, for most of the measured variables, there were no animal species x diet interactions. Thus, goats and wethers had similar ruminal activities when fed diets based on olive leaves.

  • ruminal fermentation and degradation patterns protozoa population and urinary purine derivatives excretion in goats and wethers fed diets based on two stage olive cake effect of peg supply
    Journal of Animal Science, 2004
    Co-Authors: D Yanez R Ruiz, A Martin I Garcia, Ahmed El Moumen, M.e. Vergara Alcaide
    Abstract:

    : Three experiments were conducted in Granadina goats and Segurena wethers fed at maintenance level to evaluate the effect of including a mixture of barley and a new by-product derived from olive oil extraction (two-stage dried olive cake) on ruminal degradation and passage kinetics (Exp. 1), fermentation pattern and protozoa population (Exp. 2), and urinary purine derivatives excretion (Exp. 3). Polyethylene glycol was supplied to the animals to evaluate the effects of tannins contained in the by-product. The experimental diets were as follows: alfalfa hay and alfalfa hay plus a concentrate, formulated with two-stage dried olive cake, barley, and a mineral-vitamin mixture either with or without the addition of polyethylene glycol to the drinking water. The inclusion of two-stage dried olive cake in the diet resulted in an increase of condensed tannins. Ruminal VFA concentration in goats and wethers increased (P < 0.05) and ammonia N (NH3-N) concentration decreased (P < 0.05). The inclusion of two-stage dried olive cake decreased (P < 0.001) urinary allantoin excretion only in wethers. Ruminal degradation profiles and fractional passage rates were similar in goats and wethers. The polyethylene glycol supply increased (P < 0.001) DM and N degradation rates in both animal species but did not modify the fractional passage rate. Ruminal fermentation patterns were also similar in goats and wethers and were affected by polyethylene glycol supply. In general, Entodiniomorphida and Holotricha protozoa counts were higher (P < 0.05) in the rumen of goats than of wethers. Protozoa count in wethers responded more to polyethylene glycol supply than in goats. The present work presents the first data obtained from a comparative study with sheep and goats concerning urinary excretion of purine derivatives. The excretion was similar in both animal species when fed alfalfa hay; however, polyethylene glycol affected only urinary allantoin excretion in wethers. Results suggest a greater sensitivity of wethers than of goats to two-stage olive cake condensed tannins.

Howard D Thames - One of the best experts on this subject based on the ideXlab platform.

Ricardo Andrade Reis - One of the best experts on this subject based on the ideXlab platform.

  • inoculation of corn silage with lactobacillus plantarum and bacillus subtilis associated with amylolytic enzyme supply at feeding 1 feed intake apparent digestibility and microbial protein synthesis in wethers
    Animal Feed Science and Technology, 2018
    Co-Authors: E. C. Lara, Carlos Henrique Silveira Rabelo, Uly Carneiro Bragiato, Juliana Duarte Messana, Ricardo Andrade Reis
    Abstract:

    Abstract The current study aimed to investigate the effects of corn silage inoculation and dietary supplementation with amylolytic enzymes, and their interactions, on feed intake, apparent digestibility, and microbial protein synthesis in wethers. Whole-crop corn forage was ensiled either with water (untreated) or with Lactobacillus plantarum MA 18/5U at 1 × 10 5 cfu/g fresh forage combined with Bacillus subtilis AT553098 at 1 × 10 5 cfu/g fresh forage (inoculated). Eight ruminally cannulated Dorper × Santa Ines crossbred wethers were used in a double 4 × 4 Latin square design. Wethers were assigned to one of four diets, as follows: 1) untreated corn silage with no amylolytic enzyme supply (US-NE); 2) untreated corn silage with amylolytic enzyme supply (US-WE); 3) inoculated corn silage with no amylolytic enzyme supply (IS-NE); and 4) inoculated corn silage with amylolytic enzyme supply (IS-WE). α-Amylase (602 dextrinizing units/kg of dry matter (DM)) was supplied to the wethers at total mixed ration delivery. Dietary supplementation with amylolytic enzyme increased ( P  =  0.045) DM and starch ( P  =  0.014) intake in wethers fed the US diet (+245 and +136 g/d, respectively), but not in those fed the IS diet. Inoculation of corn silage ( P  =  0.026) improved organic matter (OM), crude protein (CP), neutral detergent fiber (aNDFom), and gross energy (GE) digestibility by 5.3%, 11.5%, 25.8%, and 7.1%, respectively. Likewise, enzyme supply increased ( P  =  0.042) the apparent digestibility of DM, OM, CP, aNDFom, and GE by 5.1%, 4.7%, 9.4%, 20.7%, and 6.2%, respectively. Enzyme supply increased the molar proportion of propionic acid ( P  =  0.002; 24.1 vs. 19.6 mM / 100 mM) in wethers fed the US diet, but not in those fed the IS diet. Enzyme supply decreased microbial N synthesis ( P  =  0.025; 6.33 vs. 8.05 g/d) when the wethers were fed the IS diet, but not the US diet. In conclusion, despite the improved OM digestibility, dietary supplementation with amylolytic enzyme yielded inconsistent results with respect to feed intake and microbial protein synthesis in wethers fed diets differing in the use of silage inoculant.

  • effects of lactobacillus buchneri as a silage inoculant and as a probiotic on feed intake apparent digestibility and ruminal fermentation and microbiology in wethers fed low dry matter whole crop maize silage
    Grass and Forage Science, 2018
    Co-Authors: Carlos Henrique Silveira Rabelo, Fernanda Carvalho Basso, E. C. Lara, Campbell J L Harter, L G O Jorge, Luis Felipe Prada E Silva, L G Mesquita, Ricardo Andrade Reis
    Abstract:

    Lactobacillus buchneri was investigated as a silage inoculant and as a probiotic on feed intake, apparent digestibility, and ruminal fermentation and microbiology in wethers fed low-dry-matter (DM) whole-crop maize silage. Maize forage (279 g/kg DM) was ensiled without inoculant (untreated) and with L. buchneri CNCM I-4323 at 1 × 105 cfu/g fresh forage (inoculated). Six cannulated wethers were arranged in a double 3 × 3 Latin square and assigned to one of three diets: (i) untreated maize silage (untreated), (ii) inoculated maize silage (inoculated), and (iii) untreated maize silage with a daily dose of L. buchneri (1 × 107 cfu/g supplied silage) injected directly into the rumen (LB-probiotic). Wethers fed the inoculated diet had a higher (p = .050) DM intake (1.30% body weight [BW]) than wethers fed untreated and LB-probiotic diets (1.17% and 1.18% BW respectively). The relative proportion of Ruminococcus flavefaciens (proportion of total estimated rumen bacterial 16S rDNA) in the rumen of wethers fed inoculated and LB-probiotic diets (both 0.42%) tended (p = .098) to be lower than in the untreated diet (0.83%). Lactobacillus buchneri as a silage inoculant or as a probiotic had little effect on the variables measured in wethers.

G D Snowder - One of the best experts on this subject based on the ideXlab platform.

  • collagen characteristics of skin fell and epimysium from rams wethers and zeranol implanted ram lambs
    Journal of Animal Science, 1995
    Co-Authors: Giuseppe Maiorano, Richard J Mccormick, R.a. Field, G D Snowder
    Abstract:

    Eighteen spring-born Columbia ram, wether, and zeranol-implanted ram lambs were examined to determine the influence of castration or zeranol implantation on collagen characteristics of skin, fell, and epimysium and possible relationships between collagen properties of each tissue and difficulty of pelt removal. Pelt removal force was lower in wethers than in rams (P < .05) and intermediate for zeranol-implanted rams. Collagen concentration in skin of rams was greater (P < .05) than that in wethers or implanted rams, but percentage of heat-soluble collagen in skin was higher in implanted rams. Percentage of type III collagen in skin was highest in rams and lowest in wethers (P < .05); that in the skin of implanted rams was intermediate. The fell of wethers contained a higher (P < .05) collagen concentration, higher insoluble collagen amount, and lower percentage of soluble collagen than that of rams or implanted rams. The fell had a lower percentage of type III collagen than that of rams (P < .05). Epimysium collagen concentration of rams and wethers was higher (P < .05) than that of implanted rams; however, percentage of soluble collagen was higher for the implanted rams than for the other classes. Type III collagen percentage in the epimysium did not differ by animal class (P < .05). Zeranol-implanted ram lambs had a higher percentage of soluble collagen in all tissues examined than did non-implanted rams and force required for pelt removal was reduced in implanted rams.(ABSTRACT TRUNCATED AT 250 WORDS)

  • intramuscular collagen characteristics of ram wether and zeranol implanted ram lambs
    Journal of Animal Science, 1993
    Co-Authors: G. Maiorano, Richard J Mccormick, R.a. Field, G D Snowder
    Abstract:

    Eighteen spring-born Columbia ram, wether, and zeranol-implanted ram lambs were studied to determine the influence of castration or zeranol implants on intramuscular collagen (IMC) properties and muscle shear force values. Warner-Bratzler shear force values for longissimus muscle were greatest for ram lambs, intermediate for implanted rams, and least for wethers (P < .05). Nonreducible collagen crosslink concentration was greater in IMC of rams and implanted rams (P < .05). The IMC from rams compared with that from wethers contained proportionately more Type III than Type I collagen (P < .05); values for implanted rams were intermediate. Heat-soluble muscle collagen concentration was greater for rams and implanted rams than for wethers (P < .05); however, insoluble collagen concentration did not differ by treatment. Muscle collagen concentrations were not different for rams, wethers, or implanted rams. Increased shear force values in rams were associated with elevated collagen crosslink concentration and increased proportion of Type III collagen. Greater concentration of soluble collagen in ram IMC neither diminished nor diluted IMC crosslinking. The proportion of heat-labile collagen in the fractions did not reflect the IMC crosslinking profile for ram and wether lambs. Zeranol implantation modified IMC characteristics of rams such that shear force values and some collagen properties were similar to those of wethers.