The Experts below are selected from a list of 75 Experts worldwide ranked by ideXlab platform
Yuanzhong Wang - One of the best experts on this subject based on the ideXlab platform.
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multivariate characterization of elements accumulated in Wolfiporia extensa mushroom from yunnan province of china
Journal of Environmental Science and Health Part B-pesticides Food Contaminants and Agricultural Wastes, 2017Co-Authors: Ji Zhang, Yuanzhong WangAbstract:ABSTRACTDried sclerotia of Wolfiporia extensa have been used as medicine in Asia from Eastern Han Dynasty, and also used as traditional snack called “fulingjiabing” in Beijing, China. In this paper, 18 macro and trace elements (Ag, As, Ba, Cd, Co, Cr, Cs, Cu, Fe, Li, Mn, Ni, Pb, Rb, Se, Sr, V, and Zn) in both flesh and peel of Wolfiporia extensa from seven sites of Yunnan province in China were determined by inductively coupled plasma mass spectrometer. The average recovery rates of certified reference materials for GBW10015 (spinach leaves) ranged from 90.5 to 113%, for GBW10028 (citrus leaves) from 92.8 to 106%, and for GBW07603 (bush branch and leaves) from 83.3 to 114.6%. Generally speaking, the concentration of all elements determined was at common level. The results of this survey indicate that mineral compositions in peel were higher than in flesh. In peel, the contents of investigated trace metals in mushroom samples were found to be in the range of 1,660–13,400 µg·g−1 dry matter (dm) for Fe and 2...
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a comprehensive and comparative study of Wolfiporia extensa cultivation regions by fourier transform infrared spectroscopy and ultra fast liquid chromatography
PLOS ONE, 2016Co-Authors: Yan Li, Ji Zhang, Tao Li, Yuanzhong WangAbstract:Nowadays, Wolfiporia extensa as a popular raw material in food and medicine industry has received increasing interests. Due to supply shortage, this species of edible and medicinal mushroom has been cultivated in some provinces of China. In the present study, cultivated W. extensa collected from six regions in Yunnan Province of China were analyzed by an integrated method based on Fourier transform infrared (FT-IR) spectroscopy and ultra-fast liquid chromatography (UFLC) coupled with multivariate analysis including partial least squares discriminant analysis (PLS-DA) and hierarchical cluster analysis (HCA) in order to investigate the differences and similarities in different origins and parts. In the tested mushroom samples, characteristic FT-IR spectra were obtained for acquiring comprehensive fuzz chemical information and pachymic acid was determinated as a biomarker in the meantime. From the results, the comparison of samples was achieved successfully according to their geographical regions and different parts. All the samples displayed regional dependence and the inner parts showed better quality consistency. In addition, the chemical constituents of cultivated W. extensa could be also affected by the cultivation methods. Meanwhile, there was an interesting finding that the soil properties of cultivation regions may have a relationship with the chemical constituents of the epidermis of soil-cultured W. extensa, rather than the inner parts. Collectively, it demonstrated that the present study could provide comprehensive chemical evidence for the critical complement of quality evaluation on the cultivated W. extensa. Moreover, it may be available for the further researches of complicated mushrooms in practice.
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mercury in sclerotia of Wolfiporia extensa peck ginns fungus collected across of the yunnan land
Spectroscopy and Spectral Analysis, 2016Co-Authors: Anna Wiejak, Ji Zhang, Yuanzhong Wang, Jerzy FalandyszAbstract:: Dried sclerotia of Wolfiporia extensa has a long history of medicinal uses in Asia and also is a traditional snack in Beijing that is called “fuling jiabing”. This study aimed at providing and evaluating data on total Hg contents of sclerotia collected across of the Yunnan land in China, which is generally lacking information. Sclerotia of W. extensa showed a low contamination with Hg when compared to fruiting bodies of many mushroom species. The Hg contents ranged from 0.004 1 to 0.019 mg Hg per kg dry matter with a median value of 0.011 mg·kg-1 dry matter and an overall mean value of (0.011±0.004) mg·kg-1 dry matter. Mercury content of sclerotia varied between the places of collection in mountainous Yunnan. Assessed intake of Hg by adult eating 50 g of the “average” Yunnan’s origin sclerotia contained in the “Fuling jiabing” snack could be between 0.000 009 2 and 0.000 55 mg per capita or between 0.000 003 4 and 0.000 016 mg per kg body mass for a typical adult of mass 60 kg, which is a low intake and Hg intakes would even be much less if taking decoctions of sclerotia. This study has revealed that sclerotia of W. extensa showed a weak contamination with Hg and possible Hg intake eating sclerotia of W. extensa is below health limits. Also Hg intake from the decoctions of W. extensa is much below health limits and “fuling jiabing” snack made of sclerotia of W. extensa provides little Hg.
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ultraviolet spectroscopy combined with ultra fast liquid chromatography and multivariate statistical analysis for quality assessment of wild Wolfiporia extensa from different geographical origins
Spectrochimica Acta Part A: Molecular and Biomolecular Spectroscopy, 2016Co-Authors: Yan Li, Ji Zhang, Yuanzhong WangAbstract:A quality assessment system comprised of a tandem technique of ultraviolet (UV) spectroscopy and ultra-fast liquid chromatography (UFLC) aided by multivariate analysis was presented for the determination of geographic origin of Wolfiporia extensa collected from five regions in Yunnan Province of China. Characteristic UV spectroscopic fingerprints of samples were determined based on its methanol extract. UFLC was applied for the determination of pachymic acid (a biomarker) presented in individual test samples. The spectrum data matrix and the content of pachymic acid were integrated and analyzed by partial least squares discriminant analysis (PLS-DA) and hierarchical cluster analysis (HCA). The results showed that chemical properties of samples were clearly dominated by the epidermis and inner part as well as geographical origins. The relationships among samples obtained from these five regions have been also presented. Moreover, an interesting finding implied that geographical origins had much greater influence on the chemical properties of epidermis compared with that of the inner part. This study demonstrated that a rapid tool for accurate discrimination of W. extensa by UV spectroscopy and UFLC could be available for quality control of complicated medicinal mushrooms.
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arsenic cadmium and lead in sclerotia of Wolfiporia extensa of yunnan china
Food Additives & Contaminants Part B-surveillance, 2016Co-Authors: Ji Zhang, Yuanzhong Wang, Yan-li Zhao, Wan-yi LiAbstract:ABSTRACTConsidering the environmental pollution, edible mushroom safety is of great concern to consumers. This study aimed at providing and evaluating data on As, Cd and Pb content of sclerotia of Wolfiporia extensa collected across Yunnan in China. For the presented survey As, Cd, and Pb concentration in sclerotia of wild and cultivated W. extensa was determined by inductively coupled plasma mass spectrometry. The results showed that As, Cd, and Pb content were below the limit promulgated by WHO, with the ranges 5.27–161, 1.51–42.1 and < 1–634 ng g−1 dry matter. Calculated hazard quotient (HQ) and hazard index (HI) were used to evaluate the non-carcinogenic health risk from individual and combined metals via daily consumption of 50 g sclerotia. Both HQ and HI through consumption of sclerotia were below 1, indicating that weekly consumption of sclerotia at the indicated doses poses no significant health risk to an adult consumer.
Ji Zhang - One of the best experts on this subject based on the ideXlab platform.
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multivariate characterization of elements accumulated in Wolfiporia extensa mushroom from yunnan province of china
Journal of Environmental Science and Health Part B-pesticides Food Contaminants and Agricultural Wastes, 2017Co-Authors: Ji Zhang, Yuanzhong WangAbstract:ABSTRACTDried sclerotia of Wolfiporia extensa have been used as medicine in Asia from Eastern Han Dynasty, and also used as traditional snack called “fulingjiabing” in Beijing, China. In this paper, 18 macro and trace elements (Ag, As, Ba, Cd, Co, Cr, Cs, Cu, Fe, Li, Mn, Ni, Pb, Rb, Se, Sr, V, and Zn) in both flesh and peel of Wolfiporia extensa from seven sites of Yunnan province in China were determined by inductively coupled plasma mass spectrometer. The average recovery rates of certified reference materials for GBW10015 (spinach leaves) ranged from 90.5 to 113%, for GBW10028 (citrus leaves) from 92.8 to 106%, and for GBW07603 (bush branch and leaves) from 83.3 to 114.6%. Generally speaking, the concentration of all elements determined was at common level. The results of this survey indicate that mineral compositions in peel were higher than in flesh. In peel, the contents of investigated trace metals in mushroom samples were found to be in the range of 1,660–13,400 µg·g−1 dry matter (dm) for Fe and 2...
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a comprehensive and comparative study of Wolfiporia extensa cultivation regions by fourier transform infrared spectroscopy and ultra fast liquid chromatography
PLOS ONE, 2016Co-Authors: Yan Li, Ji Zhang, Tao Li, Yuanzhong WangAbstract:Nowadays, Wolfiporia extensa as a popular raw material in food and medicine industry has received increasing interests. Due to supply shortage, this species of edible and medicinal mushroom has been cultivated in some provinces of China. In the present study, cultivated W. extensa collected from six regions in Yunnan Province of China were analyzed by an integrated method based on Fourier transform infrared (FT-IR) spectroscopy and ultra-fast liquid chromatography (UFLC) coupled with multivariate analysis including partial least squares discriminant analysis (PLS-DA) and hierarchical cluster analysis (HCA) in order to investigate the differences and similarities in different origins and parts. In the tested mushroom samples, characteristic FT-IR spectra were obtained for acquiring comprehensive fuzz chemical information and pachymic acid was determinated as a biomarker in the meantime. From the results, the comparison of samples was achieved successfully according to their geographical regions and different parts. All the samples displayed regional dependence and the inner parts showed better quality consistency. In addition, the chemical constituents of cultivated W. extensa could be also affected by the cultivation methods. Meanwhile, there was an interesting finding that the soil properties of cultivation regions may have a relationship with the chemical constituents of the epidermis of soil-cultured W. extensa, rather than the inner parts. Collectively, it demonstrated that the present study could provide comprehensive chemical evidence for the critical complement of quality evaluation on the cultivated W. extensa. Moreover, it may be available for the further researches of complicated mushrooms in practice.
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mercury in sclerotia of Wolfiporia extensa peck ginns fungus collected across of the yunnan land
Spectroscopy and Spectral Analysis, 2016Co-Authors: Anna Wiejak, Ji Zhang, Yuanzhong Wang, Jerzy FalandyszAbstract:: Dried sclerotia of Wolfiporia extensa has a long history of medicinal uses in Asia and also is a traditional snack in Beijing that is called “fuling jiabing”. This study aimed at providing and evaluating data on total Hg contents of sclerotia collected across of the Yunnan land in China, which is generally lacking information. Sclerotia of W. extensa showed a low contamination with Hg when compared to fruiting bodies of many mushroom species. The Hg contents ranged from 0.004 1 to 0.019 mg Hg per kg dry matter with a median value of 0.011 mg·kg-1 dry matter and an overall mean value of (0.011±0.004) mg·kg-1 dry matter. Mercury content of sclerotia varied between the places of collection in mountainous Yunnan. Assessed intake of Hg by adult eating 50 g of the “average” Yunnan’s origin sclerotia contained in the “Fuling jiabing” snack could be between 0.000 009 2 and 0.000 55 mg per capita or between 0.000 003 4 and 0.000 016 mg per kg body mass for a typical adult of mass 60 kg, which is a low intake and Hg intakes would even be much less if taking decoctions of sclerotia. This study has revealed that sclerotia of W. extensa showed a weak contamination with Hg and possible Hg intake eating sclerotia of W. extensa is below health limits. Also Hg intake from the decoctions of W. extensa is much below health limits and “fuling jiabing” snack made of sclerotia of W. extensa provides little Hg.
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ultraviolet spectroscopy combined with ultra fast liquid chromatography and multivariate statistical analysis for quality assessment of wild Wolfiporia extensa from different geographical origins
Spectrochimica Acta Part A: Molecular and Biomolecular Spectroscopy, 2016Co-Authors: Yan Li, Ji Zhang, Yuanzhong WangAbstract:A quality assessment system comprised of a tandem technique of ultraviolet (UV) spectroscopy and ultra-fast liquid chromatography (UFLC) aided by multivariate analysis was presented for the determination of geographic origin of Wolfiporia extensa collected from five regions in Yunnan Province of China. Characteristic UV spectroscopic fingerprints of samples were determined based on its methanol extract. UFLC was applied for the determination of pachymic acid (a biomarker) presented in individual test samples. The spectrum data matrix and the content of pachymic acid were integrated and analyzed by partial least squares discriminant analysis (PLS-DA) and hierarchical cluster analysis (HCA). The results showed that chemical properties of samples were clearly dominated by the epidermis and inner part as well as geographical origins. The relationships among samples obtained from these five regions have been also presented. Moreover, an interesting finding implied that geographical origins had much greater influence on the chemical properties of epidermis compared with that of the inner part. This study demonstrated that a rapid tool for accurate discrimination of W. extensa by UV spectroscopy and UFLC could be available for quality control of complicated medicinal mushrooms.
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arsenic cadmium and lead in sclerotia of Wolfiporia extensa of yunnan china
Food Additives & Contaminants Part B-surveillance, 2016Co-Authors: Ji Zhang, Yuanzhong Wang, Yan-li Zhao, Wan-yi LiAbstract:ABSTRACTConsidering the environmental pollution, edible mushroom safety is of great concern to consumers. This study aimed at providing and evaluating data on As, Cd and Pb content of sclerotia of Wolfiporia extensa collected across Yunnan in China. For the presented survey As, Cd, and Pb concentration in sclerotia of wild and cultivated W. extensa was determined by inductively coupled plasma mass spectrometry. The results showed that As, Cd, and Pb content were below the limit promulgated by WHO, with the ranges 5.27–161, 1.51–42.1 and < 1–634 ng g−1 dry matter. Calculated hazard quotient (HQ) and hazard index (HI) were used to evaluate the non-carcinogenic health risk from individual and combined metals via daily consumption of 50 g sclerotia. Both HQ and HI through consumption of sclerotia were below 1, indicating that weekly consumption of sclerotia at the indicated doses poses no significant health risk to an adult consumer.
Yan Li - One of the best experts on this subject based on the ideXlab platform.
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a comprehensive and comparative study of Wolfiporia extensa cultivation regions by fourier transform infrared spectroscopy and ultra fast liquid chromatography
PLOS ONE, 2016Co-Authors: Yan Li, Ji Zhang, Tao Li, Yuanzhong WangAbstract:Nowadays, Wolfiporia extensa as a popular raw material in food and medicine industry has received increasing interests. Due to supply shortage, this species of edible and medicinal mushroom has been cultivated in some provinces of China. In the present study, cultivated W. extensa collected from six regions in Yunnan Province of China were analyzed by an integrated method based on Fourier transform infrared (FT-IR) spectroscopy and ultra-fast liquid chromatography (UFLC) coupled with multivariate analysis including partial least squares discriminant analysis (PLS-DA) and hierarchical cluster analysis (HCA) in order to investigate the differences and similarities in different origins and parts. In the tested mushroom samples, characteristic FT-IR spectra were obtained for acquiring comprehensive fuzz chemical information and pachymic acid was determinated as a biomarker in the meantime. From the results, the comparison of samples was achieved successfully according to their geographical regions and different parts. All the samples displayed regional dependence and the inner parts showed better quality consistency. In addition, the chemical constituents of cultivated W. extensa could be also affected by the cultivation methods. Meanwhile, there was an interesting finding that the soil properties of cultivation regions may have a relationship with the chemical constituents of the epidermis of soil-cultured W. extensa, rather than the inner parts. Collectively, it demonstrated that the present study could provide comprehensive chemical evidence for the critical complement of quality evaluation on the cultivated W. extensa. Moreover, it may be available for the further researches of complicated mushrooms in practice.
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ultraviolet spectroscopy combined with ultra fast liquid chromatography and multivariate statistical analysis for quality assessment of wild Wolfiporia extensa from different geographical origins
Spectrochimica Acta Part A: Molecular and Biomolecular Spectroscopy, 2016Co-Authors: Yan Li, Ji Zhang, Yuanzhong WangAbstract:A quality assessment system comprised of a tandem technique of ultraviolet (UV) spectroscopy and ultra-fast liquid chromatography (UFLC) aided by multivariate analysis was presented for the determination of geographic origin of Wolfiporia extensa collected from five regions in Yunnan Province of China. Characteristic UV spectroscopic fingerprints of samples were determined based on its methanol extract. UFLC was applied for the determination of pachymic acid (a biomarker) presented in individual test samples. The spectrum data matrix and the content of pachymic acid were integrated and analyzed by partial least squares discriminant analysis (PLS-DA) and hierarchical cluster analysis (HCA). The results showed that chemical properties of samples were clearly dominated by the epidermis and inner part as well as geographical origins. The relationships among samples obtained from these five regions have been also presented. Moreover, an interesting finding implied that geographical origins had much greater influence on the chemical properties of epidermis compared with that of the inner part. This study demonstrated that a rapid tool for accurate discrimination of W. extensa by UV spectroscopy and UFLC could be available for quality control of complicated medicinal mushrooms.
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exploring geographical differentiation of the hoelen medicinal mushroom Wolfiporia extensa agaricomycetes using fourier transform infrared spectroscopy combined with multivariate analysis
International Journal of Medicinal Mushrooms, 2016Co-Authors: Yan Li, Ji Zhang, Yan-li Zhao, Yuanzhong WangAbstract:: In this study the geographical differentiation of dried sclerotia of the medicinal mushroom Wolfiporia extensa, obtained from different regions in Yunnan Province, China, was explored using Fourier-transform infrared (FT-IR) spectroscopy coupled with multivariate data analysis. The FT-IR spectra of 97 samples were obtained for wave numbers ranging from 4000 to 400 cm-1. Then, the fingerprint region of 1800-600 cm-1 of the FT-IR spectrum, rather than the full spectrum, was analyzed. Different pretreatments were applied on the spectra, and a discriminant analysis model based on the Mahalanobis distance was developed to select an optimal pretreatment combination. Two unsupervised pattern recognition procedures- principal component analysis and hierarchical cluster analysis-were applied to enhance the authenticity of discrimination of the specimens. The results showed that excellent classification could be obtained after optimizing spectral pretreatment. The tested samples were successfully discriminated according to their geographical locations. The chemical properties of dried sclerotia of W. extensa were clearly dependent on the mushroom's geographical origins. Furthermore, an interesting finding implied that the elevations of collection areas may have effects on the chemical components of wild W. extensa sclerotia. Overall, this study highlights the feasibility of FT-IR spectroscopy combined with multivariate data analysis in particular for exploring the distinction of different regional W. extensa sclerotia samples. This research could also serve as a basis for the exploitation and utilization of medicinal mushrooms.
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characteristic fingerprint based on low polar constituents for discrimination of Wolfiporia extensa according to geographical origin using uv spectroscopy and chemometrics methods
Journal of Automated Methods & Management in Chemistry, 2014Co-Authors: Yan Li, Ji Zhang, Yan-li Zhao, Zhimin Li, Tao Li, Yuanzhong WangAbstract:The fungus species Wolfiporia extensa has a long history of medicinal usage and has also been commercially used to formulate nutraceuticals and functional foods in certain Asian countries. In the present study, a practical and promising method has been developed to discriminate the dried sclerotium of W. extensa collected from different geographical sites based on UV spectroscopy together with chemometrics methods. Characteristic fingerprint of low polar constituents of sample extracts that originated from chloroform has been obtained in the interval 250–400 nm. Chemometric pattern recognition methods such as partial least squares discriminant analysis (PLS-DA) and hierarchical cluster analysis (HCA) were applied to enhance the authenticity of discrimination of the specimens. The results showed that W. extensa samples were well classified according to their geographical origins. The proposed method can fully utilize diversified fingerprint characteristics of sclerotium of W. extensa and requires low-cost equipment and short-time analysis in comparison with other techniques. Meanwhile, this simple and efficient method may serve as a basis for the authentication of other medicinal fungi.
Tao Li - One of the best experts on this subject based on the ideXlab platform.
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a comprehensive and comparative study of Wolfiporia extensa cultivation regions by fourier transform infrared spectroscopy and ultra fast liquid chromatography
PLOS ONE, 2016Co-Authors: Yan Li, Ji Zhang, Tao Li, Yuanzhong WangAbstract:Nowadays, Wolfiporia extensa as a popular raw material in food and medicine industry has received increasing interests. Due to supply shortage, this species of edible and medicinal mushroom has been cultivated in some provinces of China. In the present study, cultivated W. extensa collected from six regions in Yunnan Province of China were analyzed by an integrated method based on Fourier transform infrared (FT-IR) spectroscopy and ultra-fast liquid chromatography (UFLC) coupled with multivariate analysis including partial least squares discriminant analysis (PLS-DA) and hierarchical cluster analysis (HCA) in order to investigate the differences and similarities in different origins and parts. In the tested mushroom samples, characteristic FT-IR spectra were obtained for acquiring comprehensive fuzz chemical information and pachymic acid was determinated as a biomarker in the meantime. From the results, the comparison of samples was achieved successfully according to their geographical regions and different parts. All the samples displayed regional dependence and the inner parts showed better quality consistency. In addition, the chemical constituents of cultivated W. extensa could be also affected by the cultivation methods. Meanwhile, there was an interesting finding that the soil properties of cultivation regions may have a relationship with the chemical constituents of the epidermis of soil-cultured W. extensa, rather than the inner parts. Collectively, it demonstrated that the present study could provide comprehensive chemical evidence for the critical complement of quality evaluation on the cultivated W. extensa. Moreover, it may be available for the further researches of complicated mushrooms in practice.
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characteristic fingerprint based on low polar constituents for discrimination of Wolfiporia extensa according to geographical origin using uv spectroscopy and chemometrics methods
Journal of Automated Methods & Management in Chemistry, 2014Co-Authors: Yan Li, Ji Zhang, Yan-li Zhao, Zhimin Li, Tao Li, Yuanzhong WangAbstract:The fungus species Wolfiporia extensa has a long history of medicinal usage and has also been commercially used to formulate nutraceuticals and functional foods in certain Asian countries. In the present study, a practical and promising method has been developed to discriminate the dried sclerotium of W. extensa collected from different geographical sites based on UV spectroscopy together with chemometrics methods. Characteristic fingerprint of low polar constituents of sample extracts that originated from chloroform has been obtained in the interval 250–400 nm. Chemometric pattern recognition methods such as partial least squares discriminant analysis (PLS-DA) and hierarchical cluster analysis (HCA) were applied to enhance the authenticity of discrimination of the specimens. The results showed that W. extensa samples were well classified according to their geographical origins. The proposed method can fully utilize diversified fingerprint characteristics of sclerotium of W. extensa and requires low-cost equipment and short-time analysis in comparison with other techniques. Meanwhile, this simple and efficient method may serve as a basis for the authentication of other medicinal fungi.
Seung-chun Park - One of the best experts on this subject based on the ideXlab platform.
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Morphological characteristics of fruit bodies and basidiospores of Wolfiporia extensa
Journal of Mushroom, 2017Co-Authors: Woo-sik Jo, Min-gu Kang, Seung-chun ParkAbstract:Wolfiporia cocos is a well-known traditional medicine in China, Japan, Korea, and other Asian countries owing to its numerous therapeutic properties. With the aim to determine the morphology and genetic characteristics of W. cocosten strains of W. cocos were cultivated in vitro, and subsequently, rapid amplification of polymorphic DNA was performed. To the best of our knowledge, this is the first study to examine the morphology of fruit bodies of W. cocos in Korea. W. cocos were cultured on PDA agar at different temperatures (12, 16, 20, 24, and 28oC) under 12-hour light (600 Lux) / 12-hour dark photoperiod condition for 1 month. Appearance of fruit body was the highest at 28°C condition in all the strains investigated. Honeycomb-like structure on sclerotia was observed in Andong 01, Andong 02, Andong 03, KFRI 1104, KFRI 1105, KFRI 1106, KFRI 1107, KFRI 1108, and ASI 13007 strains of . The KFRI 1103 strain formed cosmos petal-like structure on sclerotia. The average size of basidiospores was recorded as 7.55 μm in height and 3.35 μ in width.
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comparison of ingredients and antioxidant activity of the domestic regional Wolfiporia extensa
The Korean Journal of Mycology, 2016Co-Authors: Suhee Choi, Woo-sik Jo, Jongwoon Choi, Seung-chun ParkAbstract:본 연구에서는 복령의 성장 환경조건이 다른 지역(강원도, 경상도, 전라도)별 복령의 성분 분석 및 생리활성 비교를 위해 연구하였다. 일반성분 분석 결과, oxygen 46~48%로 가장 많이 포함하고 있었고 다음으로 38~39%의 Carbon, 6.05~6.1% Hydrogen, 0.17~0.21% Nitrogen으로 나타났다. 그리고 3개도 복령 모두에서 sulphur 성분은 발견되지 않았다. 지역별 복령을 inductively coupled plasma-mass spectrometry(ICP) 분석으로 11종의 무기질을 분석한 결과 S는 145~149 ppm, Mg은 69~72 ppm, P는 122~154 ppm로 비슷한 함유량을 나타냈고, Ca은 경상도(509.98 ppm)가 강원도(210.61 ppm)와 전라도 (223.88 ppm)보다 약 2~2.35배보다 많이 함유하였다. GC-MS 분석 결과 모든 복령에서 oleic aid 계열의 지방산이 확인되었다. 50% 에탄올 복령 추출물의 DPPH와 ABTS radical 소거활성과 FRAP 방법을 실행하여 항산화 효과를 알아보았다. 복령 추출물 10mg/ml의 농도에서 강원도, 전라도 복령의 DPPH와 ABTS의 $IC_{50}$ 값은 강원도(3 mg/mL), 경상도(23.03 mg/mL), 전라도(4.16 mg/mL)와 강원도(3.52 mg/mL), 경상도(12.17 mg/mL), 전라도(7.40 mg/mL)로 계산되었다. FRAP에서 $IC_{50}$ 값은 강원도(6.59 mg/mL), 경상도(19.06 mg/mL), 전라도(18.97 mg/mL)로 계산되었다. 연구에 사용한 복령의 항산화 활성은 농도 의존적인 증가를 보였으며 강원도, 전라도, 경상도 복령의 순으로 항산화 활성이 나타났다. 【This study was conducted for comparison of ingredients, phytochemical compounds and antioxidant activity of Wofiporia extensa cultured in Gangwon-do, Gyeongsang-do, and Jeolla-do. Three contents of Wofiporia extensa were analyzed as oxygen (46~48%), carbon (38~39%), hydrogen (6.05~6.1%) and nitrogen (0.17~0.21%). The mineral contents of 50% ethanol Wofiporia extensa extracts were measured as sulfur (S) 145~149 ppm, Magnesium (Mg) 69~72 ppm, phosphorus (P) 122~154 ppm and calcium (Ca) 210.61~509.98 ppm. Wofiporia extensa from Gyeongsang-do (509.98 ppm) contained a significantly higher quantity of Ca than that from Gangwon-do (210.62 ppm) and Jeolla-do (223.88 ppm). In the gas chromatograph-mass spectrometry (GC-MS) analysis, oleic acid was identified in three 50% ethanol Wofiporia extensa extracts. In the 1,1-diphenyl-2-picrylhydrazyl (DPPH) and 2,2'-azino-bis-3-ethylbenzothiazoline-6-sulphonic acid (ABTS) assay for antioxidant activity, the $IC_{50}$ values of Wofiporia extensa cultured in Gangwon-do, Gyeongsang-do and Jeolla-do were calculated as 2.966 mg/mL, 23.03 mg/mL, and 4.16 mg/mL and 3.521 mg/mL, 12.17 mg/mL, and 7.40 mg/mL. In the ferric reducing antioxidant power (FRAP) assay, the $IC_{50}$ values of Wofiporia extensa cultured in Gangwon-do, Gyeongsang-do, Jeolla-do were 6.585 mg/mL, 19.06 mg/mL, and 18.97 mg/mL, respectively. In summary, Wofiporia extensa cultured in Gangwon-do had stronger antioxidant activity and higher concentration of oleic acid than that of Geyongsang-do and Jeolla-do. However, Wofiporia extensa cultured in Geyongsang-do contained a much higher concentration of Ca than that of Gangwon-do and Jeolla-do.】
