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Gebol Huallpacusi, Edith Merylou - One of the best experts on this subject based on the ideXlab platform.
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Estimación del contenido de carbono en la biomasa aérea de las especies Virola Elongata y Xylopia Ligustrifolia de un aguajal renacal en la reserva ecológica de Tingana, San Martin
'Universidad Cesar Vallejo', 2018Co-Authors: Gebol Huallpacusi, Edith MerylouAbstract:En la presente investigación se determinó el contenido de carbono en la biomasa aérea en las especies Virola elongata “Cumala” y Xylopia ligustrigolia “Espintana” presente en el ecosistema de Aguajal Renacal de la Reserva Ecológica de Tingana (2867.74 ha). Para la determinación de especies se procedió a la identificación con apoyo de los guías de la reserva y posteriormente se realizó la recolección de información de datos biométricos. La muestra de estudio es constituida por los individuos de las especies Virola elongata “Cumala” y Xylopia ligustrifolia “Espintana”, en un bosque de terraza baja presente en 10 hectáreas. Se empleó un muestreo estratificado para la ubicación de las 6 parcelas: 4 parcelas de 1 hectárea (100x100m), 1 parcela de 2 hectáreas (140x160m) y 1 parcela de 4 hectáreas (200x200m); de acuerdo a la accesibilidad según las condiciones del terreno y un muestro indirecto para la toma de datos biométricos (DAP>30cm y altura) para aplicarlos en formulas alométricas para determinar la biomasa aérea de ambas especies. La altura se midió con ayuda de varas graduadas de 1.5 metros y el DAP>30 cm se midió con ayuda de una cinta métrica a 1.30 metros a partir de la base del suelo. La captura de carbono contenida en biomasa aérea de las especies Virola elongata “Cumala” fue 10.37 MgC/ha y Xylopia ligustrifolia “Espintana” fue 1.79 MgC/ha. El contenido de carbono se obtuvo multiplicando la biomasa aérea por la concentración de carbono 0.50 (IPCC, 1996)
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Estimación del contenido de carbono en la biomasa aérea de las especies Virola Elongata y Xylopia Ligustrifolia de un aguajal renacal en la reserva ecológica de Tingana, San Martin
'Universidad Cesar Vallejo', 2018Co-Authors: Gebol Huallpacusi, Edith MerylouAbstract:TesisLima NorteEscuela Profesional de Ingeniería AmbientalGestión de Riesgo y Adaptación al Cambio ClimáticoEn la presente investigación se determinó el contenido de carbono en la biomasa aérea en las especies Virola elongata “Cumala” y Xylopia ligustrigolia “Espintana” presente en el ecosistema de Aguajal Renacal de la Reserva Ecológica de Tingana (2867.74 ha). Para la determinación de especies se procedió a la identificación con apoyo de los guías de la reserva y posteriormente se realizó la recolección de información de datos biométricos. La muestra de estudio es constituida por los individuos de las especies Virola elongata “Cumala” y Xylopia ligustrifolia “Espintana”, en un bosque de terraza baja presente en 10 hectáreas. Se empleó un muestreo estratificado para la ubicación de las 6 parcelas: 4 parcelas de 1 hectárea (100x100m), 1 parcela de 2 hectáreas (140x160m) y 1 parcela de 4 hectáreas (200x200m); de acuerdo a la accesibilidad según las condiciones del terreno y un muestro indirecto para la toma de datos biométricos (DAP>30cm y altura) para aplicarlos en formulas alométricas para determinar la biomasa aérea de ambas especies. La altura se midió con ayuda de varas graduadas de 1.5 metros y el DAP>30 cm se midió con ayuda de una cinta métrica a 1.30 metros a partir de la base del suelo. La captura de carbono contenida en biomasa aérea de las especies Virola elongata “Cumala” fue 10.37 MgC/ha y Xylopia ligustrifolia “Espintana” fue 1.79 MgC/ha. El contenido de carbono se obtuvo multiplicando la biomasa aérea por la concentración de carbono 0.50 (IPCC, 1996)
Alaide Braga De Oliveira - One of the best experts on this subject based on the ideXlab platform.
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phytochemistry and antiplasmodial activity of Xylopia sericea leaves
Natural Product Research, 2020Co-Authors: Douglas Costa Gontijo, Geraldo Celio Brandao, Maria Fernanda Alves Do Nascimento, Alaide Braga De OliveiraAbstract:Aiming to investigate the antiplasmodial activity and the phytochemical composition of Xylopia sericea leaves, the essential oil and dichloromethane extract were analyzed by gas and liquid chromato...
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antiplasmodial activity and cytotoxicity isolation of active alkaloids and dereplication of Xylopia sericea leaves ethanol extract by uplc dad esi ms ms
Journal of Pharmacy and Pharmacology, 2019Co-Authors: Douglas Costa Gontijo, Geraldo Celio Brandao, Maria Fernanda Alves Do Nascimento, Alaide Braga De OliveiraAbstract:Objectives To assess the antiplasmodial activity of the ethanol extract of Xylopia sericea leaves, Annonaceae, often associated with antimalarial use and to perform a bioguided isolation of active compounds. Methods Dereplication of ethanol extract by the UPLC-DAD-ESI-MS/MS technique allowed the identification of the major constituents, isolation and identification of alkaloids. The antiplasmodial and cytotoxic activity of the extract, fractions and isolated compounds was evaluated against the chloroquine-resistant W2 strain Plasmodium falciparum and HepG2 cells, respectively. Key findings Ethanol extract showed high reduction of parasitemia as well as moderate cytotoxicity (86.5 ± 3.0% growth inhibition at 50 μg/ml and CC50 72.1 ± 5.1 μg/ml, respectively). A total of eight flavonoids were identified, and two aporphine alkaloids, anonaine and O-methylmoschatoline, were isolated. Anonaine disclosed significant antiplasmodial effect and moderate cytotoxicity (IC50 23.2 ± 2.7 μg/ml, CC50 38.3 ± 2.3 μg/ml, SI 1.6) while O-methylmoschatoline was not active against P. falciparum and showed a low cytotoxicity (33.5 ± 1.9% growth inhibition at 50 μg/ml, CC50 274.4 ± 0.5 μg/ml). Conclusions Characterization of Xylopia sericea leaves ethanol extract by UPLC-DAD-ESI-MS/MS as well as its antiplasmodial activity and the occurrence of anonaine and O-methylmoschatoline in this Xylopia species are reported by the first time.
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mono and diterpenes from seeds of Xylopia sericea
Química Nova, 2001Co-Authors: Jacqueline A Takahashi, Maria Amelia D Boaventura, Henriete S Vieira, James R Hanson, Peter B Hitchcock, Alaide Braga De OliveiraAbstract:A monoterpene, 3b,6b-dihydroxy-p-menth-1-ene has been isolated from the seeds of Xylopia sericea along with four kaurane, one beyerene, one atisene and four trachylobane diterpenoids, including the trachyloban-18- and 19-methyl esters. The X-ray crystal structure of methyl ent-trachyloban-18-oate was determined in order to make an unambiguous distinction between the 18- and 19-esters. The 13C NMR data for ent-15a-hydroxy-trachyloban-19-oic acid has been revised.
Elvis Ofori Ameyaw - One of the best experts on this subject based on the ideXlab platform.
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isobolographic analysis of co administration of two plant derived antiplasmodial drug candidates cryptolepine and xylopic acid in plasmodium berghei
Malaria Journal, 2018Co-Authors: Elvis Ofori Ameyaw, Kodwo B Asmah, Robert Peter Biney, Isaac Tabiri Henneh, Phyllis Owusuagyei, James Prah, Arnold Donkor ForkuoAbstract:Increasing resistance to current anti-malarial therapies requires a renewed effort in searching for alternative therapies to combat this challenge, and combination therapy is the preferred approach to address this. The present study confirms the anti-plasmodial effects of two compounds, cryptolepine and xylopic acid and the relationship that exists in their combined administration determined. Anti-plasmodial effect of cryptolepine (CYP) (3, 10, 30 mg kg−1) and xylopic acid (XA) (3, 10, 30 mg kg−1) was evaluated in Plasmodium berghei-infected male mice after a 6-day drug treatment. The respective doses which produced 50% chemosuppression (ED50) was determined by iterative fitting of the log-dose responses of both drugs. CYP and XA were then co-administered in a fixed dose combination of their ED50s (1:1) as well as different fractions of these combinations (1/2, 1/4, 1/8, 1/16 and 1/32) to find the experimental ED50 (Zexp). The nature of interaction between cryptolepine and xylopic acid was determined by constructing an isobologram to compare the Zexp with the theoretical ED50 (Zadd). Additionally, the effect of cryptolepine/xylopic acid co-administration on vital organs associated with malarial parasiticidal action was assessed. The Zadd and Zexp were determined to be 12.75 ± 0.33 and 2.60 ± 0.41, respectively, with an interaction index of 0.2041. The Zexp was significantly (P < 0.001) below the additive isobole indicating that co-administration of cryptolepine and xylopic acid yielded a synergistic anti-plasmodial effect. This observed synergistic antiplasmodial effect did not have any significant deleterious effect on the kidney, liver and spleen. However, the testis were affected at high doses. The co-administration of cryptolepine and xylopic acid produces synergistic anti-malarial effect with minimal toxicity.
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Isobolographic analysis of co-administration of two plant-derived antiplasmodial drug candidates, cryptolepine and xylopic acid, in Plasmodium berghei
'Springer Science and Business Media LLC', 2018Co-Authors: Elvis Ofori Ameyaw, Kodwo B Asmah, Robert Peter Biney, Isaac Tabiri Henneh, James Prah, Phyllis Owusu-agyei, Arnold Donkor ForkuoAbstract:Abstract Background Increasing resistance to current anti-malarial therapies requires a renewed effort in searching for alternative therapies to combat this challenge, and combination therapy is the preferred approach to address this. The present study confirms the anti-plasmodial effects of two compounds, cryptolepine and xylopic acid and the relationship that exists in their combined administration determined. Methods Anti-plasmodial effect of cryptolepine (CYP) (3, 10, 30 mg kg−1) and xylopic acid (XA) (3, 10, 30 mg kg−1) was evaluated in Plasmodium berghei-infected male mice after a 6-day drug treatment. The respective doses which produced 50% chemosuppression (ED50) was determined by iterative fitting of the log-dose responses of both drugs. CYP and XA were then co-administered in a fixed dose combination of their ED50s (1:1) as well as different fractions of these combinations (1/2, 1/4, 1/8, 1/16 and 1/32) to find the experimental ED50 (Zexp). The nature of interaction between cryptolepine and xylopic acid was determined by constructing an isobologram to compare the Zexp with the theoretical ED50 (Zadd). Additionally, the effect of cryptolepine/xylopic acid co-administration on vital organs associated with malarial parasiticidal action was assessed. Results The Zadd and Zexp were determined to be 12.75 ± 0.33 and 2.60 ± 0.41, respectively, with an interaction index of 0.2041. The Zexp was significantly (P
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effects of an ethanol extract and the diterpene xylopic acid of Xylopia aethiopica fruits in murine models of musculoskeletal pain
Pharmaceutical Biology, 2016Co-Authors: Eric Woode, Elvis Ofori Ameyaw, Eric Boakyegyasi, Wonder Kofi Mensah Abotsi, James Oppong Kyekyeku, Reimmel Kwame Adosraku, Robert Peter BineyAbstract:AbstractContext: Fruits of Xylopia aethiopica (Dunal) A. Rich. (Annonaceae) are used traditionally to manage arthritis, headache and other pain disorders.Objective: The analgesic properties of the X. aethiopica ethanol fruit extract (XAE) and xylopic acid (XA) were evaluated in musculoskeletal pain models.Materials and methods: Acute muscle pain was induced in gastrocnemius muscle of Sprague–Dawley rats with 3% carrageenan (i.m.). Rats received XAE (30–300 mg/kg), XA (10–100 mg/kg) or morphine (1–10 mg/kg) after 12 h. Effects of XAE and XA on muscle pain were assessed by measuring post-treatment grip strength of the rats. Chronic muscle pain was similarly induced, but drug treatment was on the eighth day and effects of XAE and XA assessed with Randall–Selitto test for hyperlagesia. Acute-skeletal pain was induced in knee joints of rats with 3% carrageenan-kaolin mixture and effects determined 12-h later. Similar induction protocol was used for chronic knee pain with treatment and measurement as done for c...
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Xylopia aethiopica fruit extract exhibits antidepressant like effect via interaction with serotonergic neurotransmission in mice
Journal of Ethnopharmacology, 2016Co-Authors: Robert Peter Biney, Elvis Ofori Ameyaw, Eric Boakyegyasi, Charles Kwaku Benneh, Eric WoodeAbstract:Abstract Ethnopharmacological relevance Xylopia aethiopica has been used traditionally to treat some central nervous system disorders including epilepsy. Aim of the study Despite the central analgesic and sedative effects, there is little evidence for its traditional use for CNS disorders. This study thus assessed the antidepressant potential of Xylopia aethiopica ethanolic fruit extract (XAE). Material and methods Antidepressant effect was assessed in the forced swim test (FST) and tail suspension test (TST) models in mice. The role of monoamines in the antidepressant effects of XAE was evaluated by selective depletion of serotonin and noradrenaline, whereas involvement of NMDA/nitric oxide was assessed with NMDA receptor co-modulators; d -serine and d -cycloserine and NOS inhibitor, l -NAME. Results Xylopia aethiopica (30, 100, 300 mg kg−1) dose dependently reduced immobility in both FST and TST. The reduced immobility was reversed after 5-hydroxytryptamine (5-HT) depletion with tryptophan hydroxylase inhibitor—p-chlorophenylalanine (pCPA) and after monoamine depletion with vesicular monoamine transporter inhibitor—reserpine. The observed antidepressant effect was not affected by catecholamine depletion with the tyrosine hydroxylase inhibitor, α-methyl-p-tyrosine (AMPT). Similarly XAE did not potentiate the toxicity of a sub-lethal dose of noradrenaline. XAE had a synergistic effect with the glycineB receptor partial agonist, d -cycloserine and nitric oxide synthase inhibitor, l -NAME. However established antidepressant effects of XAE were abolished by NMDA and NOS activation with d -serine and l - arginine. Conclusion This study shows that Xylopia aethiopica has antidepressant potential largely due to effects on 5-HT neurotransmission with possible glutamatergic effect through the glycineB co-binding site and nitric oxide synthase inhibition.
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anti allodynic and anti hyperalgesic effects of an ethanolic extract and xylopic acid from the fruits of Xylopia aethiopica in murine models of neuropathic pain
Pharmacognosy Research, 2014Co-Authors: Elvis Ofori Ameyaw, Eric Woode, Eric Boakyegyasi, Wonder Kofi Mensah Abotsi, James Oppong Kyekyeku, Reimmel Kwame AdosrakuAbstract:Background: Fruit extracts of Xylopia aethiopica are used traditionally in the management of pain disorders including headache and neuralgia. An animal model of vincristine-induced sensory neuropathy was developed after repeated intraperitoneal injection in rats and used in the present work to study the effects of the ethanolic extract of X. aethiopica (XAE) and its diterpene xylopic acid (XA) in vincristine-induced neuropathic pain. Materials and Methods: Vincristine (0.1 mg kg -1 day -1 ) was administered during two cycles of five consecutive days to induce chemotherapy-induced neuropathic pain. Static tactile anti-allodynic, anti-hyperalgesic, and cold anti-allodynic effects of XAE (30-300 mg kg -1 ) and XA (10-100 mg kg -1 ) were assessed using Von Frey filaments of bending forces of 4, 8, and 15 g, the Randall-Selitto paw pressure test, and cold water (4.5°C), respectively. Results: Administration of vincristine caused the development of allodynia and hyperalgesia with no significant motor deficit, spontaneous pain, and foot deformity. XAE (30-300 mg kg -1 ) and XA (10-100 mg kg -1 ) exhibited anti-hyperalgesic, tactile, and cold anti-allodynic properties with XA exhibiting greater potency than XAE. Pregabalin (10-100 mg kg -1 ) used as control produced similar effect. Conclusion: These findings establish the anti-allodynic and anti-hyperalgesic effects of the ethanolic fruit XAE and its major diterpene XA in vincristine-induced neuropathtic pain.
Arnold Donkor Forkuo - One of the best experts on this subject based on the ideXlab platform.
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isobolographic analysis of co administration of two plant derived antiplasmodial drug candidates cryptolepine and xylopic acid in plasmodium berghei
Malaria Journal, 2018Co-Authors: Elvis Ofori Ameyaw, Kodwo B Asmah, Robert Peter Biney, Isaac Tabiri Henneh, Phyllis Owusuagyei, James Prah, Arnold Donkor ForkuoAbstract:Increasing resistance to current anti-malarial therapies requires a renewed effort in searching for alternative therapies to combat this challenge, and combination therapy is the preferred approach to address this. The present study confirms the anti-plasmodial effects of two compounds, cryptolepine and xylopic acid and the relationship that exists in their combined administration determined. Anti-plasmodial effect of cryptolepine (CYP) (3, 10, 30 mg kg−1) and xylopic acid (XA) (3, 10, 30 mg kg−1) was evaluated in Plasmodium berghei-infected male mice after a 6-day drug treatment. The respective doses which produced 50% chemosuppression (ED50) was determined by iterative fitting of the log-dose responses of both drugs. CYP and XA were then co-administered in a fixed dose combination of their ED50s (1:1) as well as different fractions of these combinations (1/2, 1/4, 1/8, 1/16 and 1/32) to find the experimental ED50 (Zexp). The nature of interaction between cryptolepine and xylopic acid was determined by constructing an isobologram to compare the Zexp with the theoretical ED50 (Zadd). Additionally, the effect of cryptolepine/xylopic acid co-administration on vital organs associated with malarial parasiticidal action was assessed. The Zadd and Zexp were determined to be 12.75 ± 0.33 and 2.60 ± 0.41, respectively, with an interaction index of 0.2041. The Zexp was significantly (P < 0.001) below the additive isobole indicating that co-administration of cryptolepine and xylopic acid yielded a synergistic anti-plasmodial effect. This observed synergistic antiplasmodial effect did not have any significant deleterious effect on the kidney, liver and spleen. However, the testis were affected at high doses. The co-administration of cryptolepine and xylopic acid produces synergistic anti-malarial effect with minimal toxicity.
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Isobolographic analysis of co-administration of two plant-derived antiplasmodial drug candidates, cryptolepine and xylopic acid, in Plasmodium berghei
'Springer Science and Business Media LLC', 2018Co-Authors: Elvis Ofori Ameyaw, Kodwo B Asmah, Robert Peter Biney, Isaac Tabiri Henneh, James Prah, Phyllis Owusu-agyei, Arnold Donkor ForkuoAbstract:Abstract Background Increasing resistance to current anti-malarial therapies requires a renewed effort in searching for alternative therapies to combat this challenge, and combination therapy is the preferred approach to address this. The present study confirms the anti-plasmodial effects of two compounds, cryptolepine and xylopic acid and the relationship that exists in their combined administration determined. Methods Anti-plasmodial effect of cryptolepine (CYP) (3, 10, 30 mg kg−1) and xylopic acid (XA) (3, 10, 30 mg kg−1) was evaluated in Plasmodium berghei-infected male mice after a 6-day drug treatment. The respective doses which produced 50% chemosuppression (ED50) was determined by iterative fitting of the log-dose responses of both drugs. CYP and XA were then co-administered in a fixed dose combination of their ED50s (1:1) as well as different fractions of these combinations (1/2, 1/4, 1/8, 1/16 and 1/32) to find the experimental ED50 (Zexp). The nature of interaction between cryptolepine and xylopic acid was determined by constructing an isobologram to compare the Zexp with the theoretical ED50 (Zadd). Additionally, the effect of cryptolepine/xylopic acid co-administration on vital organs associated with malarial parasiticidal action was assessed. Results The Zadd and Zexp were determined to be 12.75 ± 0.33 and 2.60 ± 0.41, respectively, with an interaction index of 0.2041. The Zexp was significantly (P
Douglas Costa Gontijo - One of the best experts on this subject based on the ideXlab platform.
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phytochemistry and antiplasmodial activity of Xylopia sericea leaves
Natural Product Research, 2020Co-Authors: Douglas Costa Gontijo, Geraldo Celio Brandao, Maria Fernanda Alves Do Nascimento, Alaide Braga De OliveiraAbstract:Aiming to investigate the antiplasmodial activity and the phytochemical composition of Xylopia sericea leaves, the essential oil and dichloromethane extract were analyzed by gas and liquid chromato...
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antiplasmodial activity and cytotoxicity isolation of active alkaloids and dereplication of Xylopia sericea leaves ethanol extract by uplc dad esi ms ms
Journal of Pharmacy and Pharmacology, 2019Co-Authors: Douglas Costa Gontijo, Geraldo Celio Brandao, Maria Fernanda Alves Do Nascimento, Alaide Braga De OliveiraAbstract:Objectives To assess the antiplasmodial activity of the ethanol extract of Xylopia sericea leaves, Annonaceae, often associated with antimalarial use and to perform a bioguided isolation of active compounds. Methods Dereplication of ethanol extract by the UPLC-DAD-ESI-MS/MS technique allowed the identification of the major constituents, isolation and identification of alkaloids. The antiplasmodial and cytotoxic activity of the extract, fractions and isolated compounds was evaluated against the chloroquine-resistant W2 strain Plasmodium falciparum and HepG2 cells, respectively. Key findings Ethanol extract showed high reduction of parasitemia as well as moderate cytotoxicity (86.5 ± 3.0% growth inhibition at 50 μg/ml and CC50 72.1 ± 5.1 μg/ml, respectively). A total of eight flavonoids were identified, and two aporphine alkaloids, anonaine and O-methylmoschatoline, were isolated. Anonaine disclosed significant antiplasmodial effect and moderate cytotoxicity (IC50 23.2 ± 2.7 μg/ml, CC50 38.3 ± 2.3 μg/ml, SI 1.6) while O-methylmoschatoline was not active against P. falciparum and showed a low cytotoxicity (33.5 ± 1.9% growth inhibition at 50 μg/ml, CC50 274.4 ± 0.5 μg/ml). Conclusions Characterization of Xylopia sericea leaves ethanol extract by UPLC-DAD-ESI-MS/MS as well as its antiplasmodial activity and the occurrence of anonaine and O-methylmoschatoline in this Xylopia species are reported by the first time.
