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Y C Chang - One of the best experts on this subject based on the ideXlab platform.
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Zantedeschia mild mosaic virus a new widespread virus in calla lily detected by elisa dot blot hybridization and ic rt pcr
Plant Pathology, 2007Co-Authors: C H Huang, T.-c. Yang, Y C ChangAbstract:A new potyvirus, Zantedeschia mild mosaic virus (ZaMMV), was recently identified in calla lily ( Zantedeschia spp.) in Taiwan. The sequenced 3 ′ -terminal region indicated a unique amino acid sequence of ZaMMV, the N terminus of the capsid protein (CP), containing 39 glutamine residues before the DAG motif. In order to obtain antiserum for subsequent studies, a recombinant ZaMMV CP without polyglutamine was successfully expressed in Escherichia coli and used as the antigen. The specificity of ZaMMV antiserum was confirmed by immunoblot and ELISA analyses. Three detection methods, ELISA, dot-blot hybridization and immunocapture reverse transcription PCR (IC-RT-PCR), were developed and were able to detect ZaMMV successfully. During 2003‐2004 field surveys, the results demonstrated that ZaMMV, as well as Zantedeschia mosaic virus (ZaMV), are prevalent in calla-growing areas. In contrast, infection by Dasheen mosaic virus (DsMV), the most important virus of aroid plants, decreased dramatically because DsMV-free calla lily seedlings were grown in the field. Accordingly, the detection methods developed in this study will be useful in studying ZaMMV and also in producing ZaMMV-free calla lilies.
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Zantedeschia mild mosaic virus, a new widespread virus in calla lily, detected by ELISA, dot‐blot hybridization and IC‐RT‐PCR
Plant Pathology, 2007Co-Authors: C H Huang, T.-c. Yang, Y C ChangAbstract:A new potyvirus, Zantedeschia mild mosaic virus (ZaMMV), was recently identified in calla lily ( Zantedeschia spp.) in Taiwan. The sequenced 3 ′ -terminal region indicated a unique amino acid sequence of ZaMMV, the N terminus of the capsid protein (CP), containing 39 glutamine residues before the DAG motif. In order to obtain antiserum for subsequent studies, a recombinant ZaMMV CP without polyglutamine was successfully expressed in Escherichia coli and used as the antigen. The specificity of ZaMMV antiserum was confirmed by immunoblot and ELISA analyses. Three detection methods, ELISA, dot-blot hybridization and immunocapture reverse transcription PCR (IC-RT-PCR), were developed and were able to detect ZaMMV successfully. During 2003‐2004 field surveys, the results demonstrated that ZaMMV, as well as Zantedeschia mosaic virus (ZaMV), are prevalent in calla-growing areas. In contrast, infection by Dasheen mosaic virus (DsMV), the most important virus of aroid plants, decreased dramatically because DsMV-free calla lily seedlings were grown in the field. Accordingly, the detection methods developed in this study will be useful in studying ZaMMV and also in producing ZaMMV-free calla lilies.
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identification and molecular characterization of Zantedeschia mild mosaic virus a new calla lily infecting potyvirus
Archives of Virology, 2005Co-Authors: C H Huang, Y C ChangAbstract:Tissue-cultured seedlings of calla lily (Zantedeschia spp.) with mild mosaic symptom were observed in Taiwan. A new potyvirus, Zantedeschia mild mosaic virus (ZaMMV), was identified in the diseased plants based on the result of ELISA, virion morphology and sequence data. In the host range test, ZaMMV only infected Philodendron selloum and Zantedeschia spp. causing systemic mosaic and mild mosaic symptom, respectively. The 3′-terminal region of the virus was amplified by RT-PCR from total RNA using a potyvirus-specific degenerate primer and an oligo-dT primer. Sequence analysis revealed that the highest amino acid sequence identity of the capsid protein (CP) gene between ZaMMV and thirty potyviruses was 55%, and the highest nucleotide identity of 3′ untranslated region (3′UTR) was 52%. Therefore, in terms of species demarcating criteria, ZaMMV is a new species of the genus Potyvirus. From the result of phylogenetic analysis, ZaMMV should be a member of the Bean common mosaic virus subgroup. According to the deduced amino acid sequence of ZaMMV, the N terminus of the CP contained 39 glutamine residues before DAG motif. This unique sequence has never been discovered in plant viruses.
C H Huang - One of the best experts on this subject based on the ideXlab platform.
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Zantedeschia mild mosaic virus a new widespread virus in calla lily detected by elisa dot blot hybridization and ic rt pcr
Plant Pathology, 2007Co-Authors: C H Huang, T.-c. Yang, Y C ChangAbstract:A new potyvirus, Zantedeschia mild mosaic virus (ZaMMV), was recently identified in calla lily ( Zantedeschia spp.) in Taiwan. The sequenced 3 ′ -terminal region indicated a unique amino acid sequence of ZaMMV, the N terminus of the capsid protein (CP), containing 39 glutamine residues before the DAG motif. In order to obtain antiserum for subsequent studies, a recombinant ZaMMV CP without polyglutamine was successfully expressed in Escherichia coli and used as the antigen. The specificity of ZaMMV antiserum was confirmed by immunoblot and ELISA analyses. Three detection methods, ELISA, dot-blot hybridization and immunocapture reverse transcription PCR (IC-RT-PCR), were developed and were able to detect ZaMMV successfully. During 2003‐2004 field surveys, the results demonstrated that ZaMMV, as well as Zantedeschia mosaic virus (ZaMV), are prevalent in calla-growing areas. In contrast, infection by Dasheen mosaic virus (DsMV), the most important virus of aroid plants, decreased dramatically because DsMV-free calla lily seedlings were grown in the field. Accordingly, the detection methods developed in this study will be useful in studying ZaMMV and also in producing ZaMMV-free calla lilies.
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Zantedeschia mild mosaic virus, a new widespread virus in calla lily, detected by ELISA, dot‐blot hybridization and IC‐RT‐PCR
Plant Pathology, 2007Co-Authors: C H Huang, T.-c. Yang, Y C ChangAbstract:A new potyvirus, Zantedeschia mild mosaic virus (ZaMMV), was recently identified in calla lily ( Zantedeschia spp.) in Taiwan. The sequenced 3 ′ -terminal region indicated a unique amino acid sequence of ZaMMV, the N terminus of the capsid protein (CP), containing 39 glutamine residues before the DAG motif. In order to obtain antiserum for subsequent studies, a recombinant ZaMMV CP without polyglutamine was successfully expressed in Escherichia coli and used as the antigen. The specificity of ZaMMV antiserum was confirmed by immunoblot and ELISA analyses. Three detection methods, ELISA, dot-blot hybridization and immunocapture reverse transcription PCR (IC-RT-PCR), were developed and were able to detect ZaMMV successfully. During 2003‐2004 field surveys, the results demonstrated that ZaMMV, as well as Zantedeschia mosaic virus (ZaMV), are prevalent in calla-growing areas. In contrast, infection by Dasheen mosaic virus (DsMV), the most important virus of aroid plants, decreased dramatically because DsMV-free calla lily seedlings were grown in the field. Accordingly, the detection methods developed in this study will be useful in studying ZaMMV and also in producing ZaMMV-free calla lilies.
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identification and molecular characterization of Zantedeschia mild mosaic virus a new calla lily infecting potyvirus
Archives of Virology, 2005Co-Authors: C H Huang, Y C ChangAbstract:Tissue-cultured seedlings of calla lily (Zantedeschia spp.) with mild mosaic symptom were observed in Taiwan. A new potyvirus, Zantedeschia mild mosaic virus (ZaMMV), was identified in the diseased plants based on the result of ELISA, virion morphology and sequence data. In the host range test, ZaMMV only infected Philodendron selloum and Zantedeschia spp. causing systemic mosaic and mild mosaic symptom, respectively. The 3′-terminal region of the virus was amplified by RT-PCR from total RNA using a potyvirus-specific degenerate primer and an oligo-dT primer. Sequence analysis revealed that the highest amino acid sequence identity of the capsid protein (CP) gene between ZaMMV and thirty potyviruses was 55%, and the highest nucleotide identity of 3′ untranslated region (3′UTR) was 52%. Therefore, in terms of species demarcating criteria, ZaMMV is a new species of the genus Potyvirus. From the result of phylogenetic analysis, ZaMMV should be a member of the Bean common mosaic virus subgroup. According to the deduced amino acid sequence of ZaMMV, the N terminus of the CP contained 39 glutamine residues before DAG motif. This unique sequence has never been discovered in plant viruses.
Malgorzata Waleron - One of the best experts on this subject based on the ideXlab platform.
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Pectobacterium Zantedeschiae sp. nov. a new species of a soft rot pathogen isolated from Calla lily (Zantedeschia spp.).
Systematic and Applied Microbiology, 2018Co-Authors: Malgorzata Waleron, Agnieszka Misztak, Martyna Franczuk, Joanna Jońca, Artur Mikiciński, Tatjana Popović, Bartosz Wielgomas, Krzysztof WaleronAbstract:Abstract Four Gram-negative, rod-shaped pectinolytic bacterial strains designated as 2M, 9M, DPMP599 and DPMP600 were subjected to polyphasic analyses that revealed their distinctiveness from the other Pectobacterium species. Strains 2M and 9M were isolated from Calla lily bulbs cultivated in Central Poland. DPMP599 and DPMP600 strains were isolated from Calla lily leaves from plants grown in Serbia. Phylogenetic analyses based on nine housekeeping genes (gapA, gyrA, icdA, pgi, proA, recA, recN, rpoA, and rpoS), as well as phylogeny based on the 381 most conserved universal proteins confirmed that Pectobacterium Zantedeschiae strains were distantly related to the other Pectobacterium, and indicated Pectobacterium atrosepticum, Pectobacterium betavasculorum, Pectobacterium parmentieri and Pectobacterium wasabiae as the closest relatives. Moreover, the analysis revealed that Pectobacterium Zantedeschiae strains are not akin to Pectobacterium aroidearum strains, which were likewise isolated from Calla lily. The genome sequencing of the strains 2M, 9M and DPMP600 and their comparison with whole genome sequences of other Pectobacterium type strains confirmed their distinctiveness and separate species status within the genus based on parameters of in silico DNA–DNA hybridization and average nucleotide identity (ANI) values. The MALDI-TOF MS proteomic profile supported the proposition of delineation of the P. Zantedeschiae and additionally confirmed the individuality of the studied strains. Based on of all of these data, it is proposed that the strains 2M, 9M, DPMP599, and DPMP600 isolated from Calla lily, previously assigned as P. atrosepticum should be reclassified as Pectobacterium Zantedeschiae sp. nov. with the strain 9MT (PCM2893 = DSM105717 = IFB9009) as the type strain.
Ewa Skutnik - One of the best experts on this subject based on the ideXlab platform.
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EFFECT OF PULSING WITH GROWTH REGULATORS ON SENESCENCE OF THE DETACHED COLD-STORED LEAVES OF Zantedeschia aethiopica Spr. AND Hosta 'Undulata Erromena'
Acta Scientiarum Polonorum-hortorum Cultus, 2005Co-Authors: Ewa Skutnik, J Rabiza-swiderAbstract:Cut leaves of Zantedeschia aethiopica Spr. and Hosta 'Undulata Erromena' are widely used as the florists' green. Effect of pulsing leaves with growth regulators and a storage method (dry or wet, in light or darkness, at 5°C) on postharvest longevity and chlorophyll content was investigated. Leaf vase life after 3 days storage was evaluated at 20°C and 12 h light/12 h night. Results confirmed that a dry and dark storage of Zant- edeschia leaves negatively affected their decorative values while for Hosta foliage a dry storage was improper both in darkness and in light. Negative effects of storage conditions could - at least partly - be alleviated by a 24 h puls conditioning with gibberellic acid or benzyladenine for Zantedeschia and Hosta, respectively. In Zantedeschia the pulsed leaves cold stored in darkness had their vase life seven-fold that of non pulsed leaves. In Hosta pulsing leaves with BA prior to storage increased their vase life six times relative to untreated leaves, however, the cytokinin did not completely overcome a negative effect of dry storage. Positive action of both growth regulators was reflected in the leaf higher chlorophyll contents at all sampling dates.
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Lipoxygenase in senescing cut leaves of Zantedeschia aethiopica Spr. and Hosta ‘Undulata erromena’ treated with GA_3 or BA
Acta Physiologiae Plantarum, 2004Co-Authors: Julita Rabiza- Wider, Aleksandra Łukaszewska, Ewa Skutnik, Zbigniew Rybka, Mariusz WachowiczAbstract:The effects of a 24 hrs pulse conditioning with GA_3 or BA on the lipoxygenase activity and the expression of the lipoxygenase gene were studied in senescing cut leaves of Zantedeschia aethiopica Spr. and Hosta ‘Undulata Erromena’, respectively. The GA_3 treatment in Zantedeschia and the BA treatment in Hosta prevented increases the activity of lipoxygenase in senescing cut leaves. No effects of growth regulators on the lipoxygenase gene expression were observed in cut leaves of both species.
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Ammonium and proline accumulation in senescing cut leaves of Zantedeschia
Acta Physiologiae Plantarum, 2004Co-Authors: Julita Rabiza- Wider, Aleksandra Łukaszewska, Ewa Skutnik, Magdalena LeszkoAbstract:Accumulation of ammonium and proline were reported as phenomena associated with plant response to stress and/or senescence. The effects of a preservative (8HQC + sucrose) and 24 hrs pulse conditioning with GA_3 on the ammonium and proline contents were studied in senescing cut leaves of Zantedeschia aethiopica Spr. and Z. elliottiana Engl., grown for the florists green. Generally, accumulation of both compounds was observed in senescing leaves, however, the final ammonium and proline levels depended upon the species and the treatment applied. Conditioning with GA_3, a treatment known to delay leaf senescence in Zantedeschia sp. , prevented the increases in the ammonium and proline contents. Standard preservative solution used to prolong the longevity of cut flowers enhanced the ammonium accumulation in senescing leaves of both species, and the proline accumulation in the leaves of Z. aethiopica , but not in Z. elliottiana . These observations suggest that neither ammonium nor proline accumulation would be fully reliable predictors of cut leaf freshness during their entire market life. However, proline accumulation could serve as a quick test of freshness in the first half of the useful market life of cut leaves of Zantedeschia .
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Effect of growth regulators on postharvest characteristics of Zantedeschia aethiopica
Postharvest Biology and Technology, 2001Co-Authors: Ewa Skutnik, A Lukaszewska, Margrethe Serek, Julita RabizaAbstract:Abstract Treating cut leaves of Zantedeschia aethiopica with aqueous solutions of gibberellic acid (GA 3 ) considerably extended their display life, whether applied as a 24 h pulse treatment, or as a brief postharvest dip. In contrast, a standard preservative solution used to prolong the longevity of cut flowers (8-HQC+sucrose) was deleterious to Zantedeschia foliage, reducing display life several fold from that of the water control. After harvest, chlorophyll content of the leaves fell more or less rapidly depending on the postharvest treatment, falling most rapidly in leaves placed in the ‘preservative’ solution and relatively gradually in leaves that had been treated with GA 3 . Leaf senescence was also associated with changes in characteristics of the cell sap, namely increased pH and electrical conductivity, parameters that may be a useful and rapid means of determining the senescence status of cut flowers during marketing.
Krzysztof Waleron - One of the best experts on this subject based on the ideXlab platform.
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Pectobacterium Zantedeschiae sp. nov. a new species of a soft rot pathogen isolated from Calla lily (Zantedeschia spp.).
Systematic and Applied Microbiology, 2018Co-Authors: Malgorzata Waleron, Agnieszka Misztak, Martyna Franczuk, Joanna Jońca, Artur Mikiciński, Tatjana Popović, Bartosz Wielgomas, Krzysztof WaleronAbstract:Abstract Four Gram-negative, rod-shaped pectinolytic bacterial strains designated as 2M, 9M, DPMP599 and DPMP600 were subjected to polyphasic analyses that revealed their distinctiveness from the other Pectobacterium species. Strains 2M and 9M were isolated from Calla lily bulbs cultivated in Central Poland. DPMP599 and DPMP600 strains were isolated from Calla lily leaves from plants grown in Serbia. Phylogenetic analyses based on nine housekeeping genes (gapA, gyrA, icdA, pgi, proA, recA, recN, rpoA, and rpoS), as well as phylogeny based on the 381 most conserved universal proteins confirmed that Pectobacterium Zantedeschiae strains were distantly related to the other Pectobacterium, and indicated Pectobacterium atrosepticum, Pectobacterium betavasculorum, Pectobacterium parmentieri and Pectobacterium wasabiae as the closest relatives. Moreover, the analysis revealed that Pectobacterium Zantedeschiae strains are not akin to Pectobacterium aroidearum strains, which were likewise isolated from Calla lily. The genome sequencing of the strains 2M, 9M and DPMP600 and their comparison with whole genome sequences of other Pectobacterium type strains confirmed their distinctiveness and separate species status within the genus based on parameters of in silico DNA–DNA hybridization and average nucleotide identity (ANI) values. The MALDI-TOF MS proteomic profile supported the proposition of delineation of the P. Zantedeschiae and additionally confirmed the individuality of the studied strains. Based on of all of these data, it is proposed that the strains 2M, 9M, DPMP599, and DPMP600 isolated from Calla lily, previously assigned as P. atrosepticum should be reclassified as Pectobacterium Zantedeschiae sp. nov. with the strain 9MT (PCM2893 = DSM105717 = IFB9009) as the type strain.
