Zonate Leaf Spot

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Hyeon Dong Shin - One of the best experts on this subject based on the ideXlab platform.

  • first report of Zonate Leaf Spot caused by grovesinia moricola on adzuki bean in korea
    Plant Disease, 2017
    Co-Authors: Sungeun Cho, Seungbeom Hong, Inyoung Choi, Hyeon Dong Shin
    Abstract:

    Adzuki bean [Vigna angularis (Willd.) Ohwi & H. Ohashi] is cultivated in more than 30 countries worldwide, and especially in East Asia. Seeds of adzuki bean are widely used as a food by at least a billion people (Xu et al. 2008). Following a prolonged period of cool and moist weather in September 2012, previously unknown symptoms of Zonated Leaf Spot with severe defoliation were observed on a local variety of adzuki bean in Goseong, Korea (38°19′51″N; 128°28′01″E). Initial symptoms included grayish-green to brownish-gray and circular to irregular shaped Leaf Spots without borderlines. As the Spots enlarged, they became creamy colored with characteristic target-shaped rings. Finally, individual Spots coalesced to form larger lesions, leading to Leaf blight and premature defoliation. Two specimens were deposited in the Korea University Herbarium (KUS-F26871 and KUS-F28950). Fruiting structures on the Leaf lesions were dominantly hypophyllous, single, hyaline, erect, easily detachable, and as long as 720 µm....

  • Zonate Leaf Spot of prunus mandshurica caused by hinomyces pruni in korea
    Journal of Phytopathology, 2015
    Co-Authors: Sungeun Cho, Ji Hyun Park, Sanghyun Lee, Chong Kyu Lee, Hyeon Dong Shin
    Abstract:

    Zonate Leaf Spots and severe defoliation were observed on Manchurian apricot (Prunus mandshurica) growing in a humid location in Korea from 2011 through 2013. The main symptoms included greyish green to brownish grey and Zonate Leaf Spots without border lines, which mostly led to premature defoliation. The morphological characteristics of the causal agent were consistent with Hinomyces pruni. Identification was supported by analysing the sequence of the internal transcribed spacer region of ribosomal DNA from an isolate. The pathogenicity of the isolate was confirmed by artificial inoculation. This is the first report of Zonate Leaf Spot caused by H. pruni on Manchurian apricot globally as well as in Korea.

  • first report of Zonate Leaf Spot caused by hinomyces moricola on japanese hop in korea
    Plant Disease, 2013
    Co-Authors: Sungeun Cho, Ji Hyun Park, Sung Hoon Hong, Hyeon Dong Shin
    Abstract:

    Japanese hop (Humulus japonicus Siebold & Zucc. = H. scandens (Lour.) Merr.), native to East Asia, is an annual, climbing or trailing vine. The vines can spread to cover large areas of open ground or low vegetation, eventually blanketing the land and vegetation. Pollen of H. japonicus is allergenic, and this species is considered as one of the important causes of pollinosis in Korea and China. It is a notorious invasive weed in the United States and also in France, Hungary, and Italy (1). In September 2012, Zonate Leaf Spots were observed on Japanese hops growing in wetlands in Yeongdong County of Korea. A voucher specimen was preserved in the Korea University Herbarium (KUS-F26901). Initial symptoms included grayish-green to grayish-brown Spots without border lines. As the lesions enlarged, they coalesced, leading to Leaf blight. Sporophores on the Leaf lesions were dominantly hypophyllous, rarely epiphyllous, solitary, erect, easily detachable, and as long as 700 μm. The upper portion of the sporophores consisted of a pyramidal head was ventricose, 320 to 520 μm long and 110 to 150 μm wide. The fungus was isolated from Leaf lesions and maintained on potato dextrose agar (PDA). Sclerotia were produced on PDA after 4 to 5 weeks at 18°C without light, but conidia were not observed in culture. These morphological and cultural characteristics were consistent with those of Hinomyces moricola (I. Hino) Narumi-Saito & Y. Harada (= Cristulariella moricola (I. Hino) Redhead) (3,4). An isolate was preserved in the Korean Agricultural Culture Collection (Accession No. KACC46955). Genomic DNA was extracted using the DNeasy Plant Mini DNA Extraction Kit (Qiagen Inc., Valencia, CA). The complete internal transcribed spacer (ITS) region of rDNA was amplified with the primers ITS1/ITS4 and sequenced. The resulting sequence of 452 bp was deposited in GenBank (Accession No. KC460209). A BLAST search in GenBank revealed that the sequence showed an exact match with those of C. moricola (JQ036181 ex Acer negundo and JQ036182 ex Glycine max). To determine the pathogenicity of the fungus, according to the procedure of Cho et al. (2), sporophores with the pyramidal head were carefully detached from a lesion on the naturally infected Leaf using a needle. Each sporophore was transferred individually onto five places of four detached healthy leaves. The leaves were placed in dew chambers and incubated at 16°C. Symptoms were observed after 2 days on all inoculated leaves. A number of sporophores and immature sclerotia which were morphologically identical to the ones observed in the field were formed on the abaxial surface of the Leaf 2 weeks after inoculation. The pathogen was reisolated from lesions on the inoculated leaves, confirming Koch's postulates. No symptoms were observed on the control leaves kept in humid chambers for 2 weeks. H. moricola was known to cause Zonate Leaf Spots and defoliation on a wide range of woody and annual plants (3). To the best of our knowledge, this is the first report of Hinomyces infection on Japanese hops in Korea. References: (1) Anonymous. Humulus japonicus (Cannabaceae): Japanese hop. Eur. Medit. Plant Prot. Org. (EPPO). 2012. (2) S. E. Cho et al. Plant Dis. 96:906, 2012. (3) D. F. Farr and A. Y. Rossman. Fungal Databases. Syst. Mycol. Microbiol. Lab., Online publication, ARS, USDA, Retrieved December 8, 2012. (4) S. A. Redhead. Can. J. Bot. 53:700, 1975.

  • first report of Zonate Leaf Spot of glycine max caused by cristulariella moricola in korea
    Plant Disease, 2012
    Co-Authors: Sungeun Cho, Ji Hyun Park, J K Choi, Hyeon Dong Shin
    Abstract:

    Soybean (Glycine max (L.) Merr.) is native to East Asia including Korea and is widely grown and consumed as an edible seed. In August 2011, following a prolonged period of cool and moist weather, Zonate Leaf Spots were observed in local soybean (cultivar unknown) planted in a mountainous area of Goseong, central Korea. A voucher specimen was collected and entered at the Korea University herbarium (KUS-F26049). Initial symptoms included grayish green-to-grayish brown Spots without border lines. As the lesions enlarged, they coalesced, leading to Leaf blight and premature defoliation. Sporophores on the Leaf lesions were dominantly hypophyllous, rarely epiphyllous, solitary, erect, easily detachable, and as long as 750 μm. The upper portion of the sporophores consisted of a pyramidal head that was ventricose, 275 to 500 μm long, and 80 to 160 μm wide. The fungus was isolated from Leaf lesions and maintained on potato dextrose agar (PDA). Sclerotia were produced on PDA after 4 to 5 weeks at 18°C without ligh...

  • Zonate Leaf Spot of acer negundo caused by cristulariella moricola in korea
    Radiation Protection Dosimetry, 2012
    Co-Authors: Sungeun Cho, Ji Hyun Park, Sanghyun Lee, Hyang Burm Lee, Hyeon Dong Shin
    Abstract:

    In early August of 2011, following a prolonged period of cool and moist weather, several trees of boxelder maple (Acer negundo) planted in Mt. Soyo located in Dongducheon, Korea, were found to be heavily damaged by premature defoliation with Zonate Leaf Spot symptoms. Numerous number of cone-shaped, white sporophores (conidia) were observed on lesions of the abaxial Leaf surface. The morphological characteristics of conidia are of typical Cristulariella moricola, which was supported by analyzing sequences of internal transcribed spacer region of ribosomal DNA. Pathogenicity of the fungus was proved by artificial inoculation in the condition of relative humidity 100% and . This is the first report of the occurrence of Zonate Leaf Spot caused by infection of C. moricola on A. negundo in Korea.

Sungeun Cho - One of the best experts on this subject based on the ideXlab platform.

  • first report of Zonate Leaf Spot caused by grovesinia moricola on adzuki bean in korea
    Plant Disease, 2017
    Co-Authors: Sungeun Cho, Seungbeom Hong, Inyoung Choi, Hyeon Dong Shin
    Abstract:

    Adzuki bean [Vigna angularis (Willd.) Ohwi & H. Ohashi] is cultivated in more than 30 countries worldwide, and especially in East Asia. Seeds of adzuki bean are widely used as a food by at least a billion people (Xu et al. 2008). Following a prolonged period of cool and moist weather in September 2012, previously unknown symptoms of Zonated Leaf Spot with severe defoliation were observed on a local variety of adzuki bean in Goseong, Korea (38°19′51″N; 128°28′01″E). Initial symptoms included grayish-green to brownish-gray and circular to irregular shaped Leaf Spots without borderlines. As the Spots enlarged, they became creamy colored with characteristic target-shaped rings. Finally, individual Spots coalesced to form larger lesions, leading to Leaf blight and premature defoliation. Two specimens were deposited in the Korea University Herbarium (KUS-F26871 and KUS-F28950). Fruiting structures on the Leaf lesions were dominantly hypophyllous, single, hyaline, erect, easily detachable, and as long as 720 µm....

  • Zonate Leaf Spot of prunus mandshurica caused by hinomyces pruni in korea
    Journal of Phytopathology, 2015
    Co-Authors: Sungeun Cho, Ji Hyun Park, Sanghyun Lee, Chong Kyu Lee, Hyeon Dong Shin
    Abstract:

    Zonate Leaf Spots and severe defoliation were observed on Manchurian apricot (Prunus mandshurica) growing in a humid location in Korea from 2011 through 2013. The main symptoms included greyish green to brownish grey and Zonate Leaf Spots without border lines, which mostly led to premature defoliation. The morphological characteristics of the causal agent were consistent with Hinomyces pruni. Identification was supported by analysing the sequence of the internal transcribed spacer region of ribosomal DNA from an isolate. The pathogenicity of the isolate was confirmed by artificial inoculation. This is the first report of Zonate Leaf Spot caused by H. pruni on Manchurian apricot globally as well as in Korea.

  • first report of Zonate Leaf Spot caused by hinomyces moricola on japanese hop in korea
    Plant Disease, 2013
    Co-Authors: Sungeun Cho, Ji Hyun Park, Sung Hoon Hong, Hyeon Dong Shin
    Abstract:

    Japanese hop (Humulus japonicus Siebold & Zucc. = H. scandens (Lour.) Merr.), native to East Asia, is an annual, climbing or trailing vine. The vines can spread to cover large areas of open ground or low vegetation, eventually blanketing the land and vegetation. Pollen of H. japonicus is allergenic, and this species is considered as one of the important causes of pollinosis in Korea and China. It is a notorious invasive weed in the United States and also in France, Hungary, and Italy (1). In September 2012, Zonate Leaf Spots were observed on Japanese hops growing in wetlands in Yeongdong County of Korea. A voucher specimen was preserved in the Korea University Herbarium (KUS-F26901). Initial symptoms included grayish-green to grayish-brown Spots without border lines. As the lesions enlarged, they coalesced, leading to Leaf blight. Sporophores on the Leaf lesions were dominantly hypophyllous, rarely epiphyllous, solitary, erect, easily detachable, and as long as 700 μm. The upper portion of the sporophores consisted of a pyramidal head was ventricose, 320 to 520 μm long and 110 to 150 μm wide. The fungus was isolated from Leaf lesions and maintained on potato dextrose agar (PDA). Sclerotia were produced on PDA after 4 to 5 weeks at 18°C without light, but conidia were not observed in culture. These morphological and cultural characteristics were consistent with those of Hinomyces moricola (I. Hino) Narumi-Saito & Y. Harada (= Cristulariella moricola (I. Hino) Redhead) (3,4). An isolate was preserved in the Korean Agricultural Culture Collection (Accession No. KACC46955). Genomic DNA was extracted using the DNeasy Plant Mini DNA Extraction Kit (Qiagen Inc., Valencia, CA). The complete internal transcribed spacer (ITS) region of rDNA was amplified with the primers ITS1/ITS4 and sequenced. The resulting sequence of 452 bp was deposited in GenBank (Accession No. KC460209). A BLAST search in GenBank revealed that the sequence showed an exact match with those of C. moricola (JQ036181 ex Acer negundo and JQ036182 ex Glycine max). To determine the pathogenicity of the fungus, according to the procedure of Cho et al. (2), sporophores with the pyramidal head were carefully detached from a lesion on the naturally infected Leaf using a needle. Each sporophore was transferred individually onto five places of four detached healthy leaves. The leaves were placed in dew chambers and incubated at 16°C. Symptoms were observed after 2 days on all inoculated leaves. A number of sporophores and immature sclerotia which were morphologically identical to the ones observed in the field were formed on the abaxial surface of the Leaf 2 weeks after inoculation. The pathogen was reisolated from lesions on the inoculated leaves, confirming Koch's postulates. No symptoms were observed on the control leaves kept in humid chambers for 2 weeks. H. moricola was known to cause Zonate Leaf Spots and defoliation on a wide range of woody and annual plants (3). To the best of our knowledge, this is the first report of Hinomyces infection on Japanese hops in Korea. References: (1) Anonymous. Humulus japonicus (Cannabaceae): Japanese hop. Eur. Medit. Plant Prot. Org. (EPPO). 2012. (2) S. E. Cho et al. Plant Dis. 96:906, 2012. (3) D. F. Farr and A. Y. Rossman. Fungal Databases. Syst. Mycol. Microbiol. Lab., Online publication, ARS, USDA, Retrieved December 8, 2012. (4) S. A. Redhead. Can. J. Bot. 53:700, 1975.

  • first report of Zonate Leaf Spot of glycine max caused by cristulariella moricola in korea
    Plant Disease, 2012
    Co-Authors: Sungeun Cho, Ji Hyun Park, J K Choi, Hyeon Dong Shin
    Abstract:

    Soybean (Glycine max (L.) Merr.) is native to East Asia including Korea and is widely grown and consumed as an edible seed. In August 2011, following a prolonged period of cool and moist weather, Zonate Leaf Spots were observed in local soybean (cultivar unknown) planted in a mountainous area of Goseong, central Korea. A voucher specimen was collected and entered at the Korea University herbarium (KUS-F26049). Initial symptoms included grayish green-to-grayish brown Spots without border lines. As the lesions enlarged, they coalesced, leading to Leaf blight and premature defoliation. Sporophores on the Leaf lesions were dominantly hypophyllous, rarely epiphyllous, solitary, erect, easily detachable, and as long as 750 μm. The upper portion of the sporophores consisted of a pyramidal head that was ventricose, 275 to 500 μm long, and 80 to 160 μm wide. The fungus was isolated from Leaf lesions and maintained on potato dextrose agar (PDA). Sclerotia were produced on PDA after 4 to 5 weeks at 18°C without ligh...

  • Zonate Leaf Spot of acer negundo caused by cristulariella moricola in korea
    Radiation Protection Dosimetry, 2012
    Co-Authors: Sungeun Cho, Ji Hyun Park, Sanghyun Lee, Hyang Burm Lee, Hyeon Dong Shin
    Abstract:

    In early August of 2011, following a prolonged period of cool and moist weather, several trees of boxelder maple (Acer negundo) planted in Mt. Soyo located in Dongducheon, Korea, were found to be heavily damaged by premature defoliation with Zonate Leaf Spot symptoms. Numerous number of cone-shaped, white sporophores (conidia) were observed on lesions of the abaxial Leaf surface. The morphological characteristics of conidia are of typical Cristulariella moricola, which was supported by analyzing sequences of internal transcribed spacer region of ribosomal DNA. Pathogenicity of the fungus was proved by artificial inoculation in the condition of relative humidity 100% and . This is the first report of the occurrence of Zonate Leaf Spot caused by infection of C. moricola on A. negundo in Korea.

O L Pereira - One of the best experts on this subject based on the ideXlab platform.

  • periconiella lecythidis sp nov the causal agent of a Zonate Leaf Spot disease of the brazilian tree lecythis pisonis
    Mycological Progress, 2008
    Co-Authors: Reginaldo Goncalves Mafia, Eraclides M Ferreira, Francisco Alves Ferreira, Uwe Braun, O L Pereira
    Abstract:

    A new defoliating disease was observed on 20-year-old trees of Lecythis pisonis in Vicosa, Minas Gerais, Brazil. The disease is characterized by forming one or more large Zonate epiphyllous Leaf Spots with a light brown to white center, followed by two to three zones gradually varying from brown to red-brown. On the corresponding abaxial Leaf surface, the Spots are lighter with a profuse greenish gray coloration caused by the fungal colonies. In PDA culture, the fungus is characterized by a slow growth of the colonies with brown-violet pigmentation at the reverse. Inoculation tests carried out on healthy plants of L. pisonis confirmed the pathogenicity of this fungus. Periconiella lecythidis sp. nov., the causal agent of the Zonate Leaf Spot disease of L. pisonis is described, illustrated, discussed and compared with the other species of the genus Periconiella. The intricate generic affinity within a complex of morphologically similar hyphomycete genera consisting of Metulocladosporiella, Parapericoniella, Penidiella and Periconiella is discussed in detail.

Weilai Meng - One of the best experts on this subject based on the ideXlab platform.

  • first report of sorghum Zonate Leaf Spot caused by gloeocercospora sorghi in china
    Plant Disease, 2017
    Co-Authors: Yu Jiang, Kejie Liu, Zhi Liu, Weilai Meng
    Abstract:

    Sorghum [Sorghum bicolor (L.) Moench] is not only an important food crop, but also used as forage, brewing and industrial raw materials in China. In October 2016, large purple-red, irregular lesions were observed on leaves of several sorghum varieties in Chaoyang city of Liaoning Province, China. The most serious symptom occurred on sorghum variety “Jiza140” and the yield losses was 30% approximately. Lesions on leaves were purple-red or dark brown color, nearly circular or irregular with 2-8 very obvious rings. Lesions occur along the Leaf margin or near the midrib with semi oval. In severe cases, lesions join to cover a large proportion of the Leaf surface. To identify the pathogen, symptomatic Leaf lesions were collected from fields and cut into small pieces, surface-sterilized in 75% ethanol for 5 s, then disinfested in 2% NaOCl for 2 min and rinsed with sterilized water. The small pieces of leaves containing lesions were placed on water agar (WA) at 25°C to allow sporulation. After incubation for 4-5...

Ji Hyun Park - One of the best experts on this subject based on the ideXlab platform.

  • Zonate Leaf Spot of prunus mandshurica caused by hinomyces pruni in korea
    Journal of Phytopathology, 2015
    Co-Authors: Sungeun Cho, Ji Hyun Park, Sanghyun Lee, Chong Kyu Lee, Hyeon Dong Shin
    Abstract:

    Zonate Leaf Spots and severe defoliation were observed on Manchurian apricot (Prunus mandshurica) growing in a humid location in Korea from 2011 through 2013. The main symptoms included greyish green to brownish grey and Zonate Leaf Spots without border lines, which mostly led to premature defoliation. The morphological characteristics of the causal agent were consistent with Hinomyces pruni. Identification was supported by analysing the sequence of the internal transcribed spacer region of ribosomal DNA from an isolate. The pathogenicity of the isolate was confirmed by artificial inoculation. This is the first report of Zonate Leaf Spot caused by H. pruni on Manchurian apricot globally as well as in Korea.

  • first report of Zonate Leaf Spot caused by hinomyces moricola on japanese hop in korea
    Plant Disease, 2013
    Co-Authors: Sungeun Cho, Ji Hyun Park, Sung Hoon Hong, Hyeon Dong Shin
    Abstract:

    Japanese hop (Humulus japonicus Siebold & Zucc. = H. scandens (Lour.) Merr.), native to East Asia, is an annual, climbing or trailing vine. The vines can spread to cover large areas of open ground or low vegetation, eventually blanketing the land and vegetation. Pollen of H. japonicus is allergenic, and this species is considered as one of the important causes of pollinosis in Korea and China. It is a notorious invasive weed in the United States and also in France, Hungary, and Italy (1). In September 2012, Zonate Leaf Spots were observed on Japanese hops growing in wetlands in Yeongdong County of Korea. A voucher specimen was preserved in the Korea University Herbarium (KUS-F26901). Initial symptoms included grayish-green to grayish-brown Spots without border lines. As the lesions enlarged, they coalesced, leading to Leaf blight. Sporophores on the Leaf lesions were dominantly hypophyllous, rarely epiphyllous, solitary, erect, easily detachable, and as long as 700 μm. The upper portion of the sporophores consisted of a pyramidal head was ventricose, 320 to 520 μm long and 110 to 150 μm wide. The fungus was isolated from Leaf lesions and maintained on potato dextrose agar (PDA). Sclerotia were produced on PDA after 4 to 5 weeks at 18°C without light, but conidia were not observed in culture. These morphological and cultural characteristics were consistent with those of Hinomyces moricola (I. Hino) Narumi-Saito & Y. Harada (= Cristulariella moricola (I. Hino) Redhead) (3,4). An isolate was preserved in the Korean Agricultural Culture Collection (Accession No. KACC46955). Genomic DNA was extracted using the DNeasy Plant Mini DNA Extraction Kit (Qiagen Inc., Valencia, CA). The complete internal transcribed spacer (ITS) region of rDNA was amplified with the primers ITS1/ITS4 and sequenced. The resulting sequence of 452 bp was deposited in GenBank (Accession No. KC460209). A BLAST search in GenBank revealed that the sequence showed an exact match with those of C. moricola (JQ036181 ex Acer negundo and JQ036182 ex Glycine max). To determine the pathogenicity of the fungus, according to the procedure of Cho et al. (2), sporophores with the pyramidal head were carefully detached from a lesion on the naturally infected Leaf using a needle. Each sporophore was transferred individually onto five places of four detached healthy leaves. The leaves were placed in dew chambers and incubated at 16°C. Symptoms were observed after 2 days on all inoculated leaves. A number of sporophores and immature sclerotia which were morphologically identical to the ones observed in the field were formed on the abaxial surface of the Leaf 2 weeks after inoculation. The pathogen was reisolated from lesions on the inoculated leaves, confirming Koch's postulates. No symptoms were observed on the control leaves kept in humid chambers for 2 weeks. H. moricola was known to cause Zonate Leaf Spots and defoliation on a wide range of woody and annual plants (3). To the best of our knowledge, this is the first report of Hinomyces infection on Japanese hops in Korea. References: (1) Anonymous. Humulus japonicus (Cannabaceae): Japanese hop. Eur. Medit. Plant Prot. Org. (EPPO). 2012. (2) S. E. Cho et al. Plant Dis. 96:906, 2012. (3) D. F. Farr and A. Y. Rossman. Fungal Databases. Syst. Mycol. Microbiol. Lab., Online publication, ARS, USDA, Retrieved December 8, 2012. (4) S. A. Redhead. Can. J. Bot. 53:700, 1975.

  • first report of Zonate Leaf Spot of glycine max caused by cristulariella moricola in korea
    Plant Disease, 2012
    Co-Authors: Sungeun Cho, Ji Hyun Park, J K Choi, Hyeon Dong Shin
    Abstract:

    Soybean (Glycine max (L.) Merr.) is native to East Asia including Korea and is widely grown and consumed as an edible seed. In August 2011, following a prolonged period of cool and moist weather, Zonate Leaf Spots were observed in local soybean (cultivar unknown) planted in a mountainous area of Goseong, central Korea. A voucher specimen was collected and entered at the Korea University herbarium (KUS-F26049). Initial symptoms included grayish green-to-grayish brown Spots without border lines. As the lesions enlarged, they coalesced, leading to Leaf blight and premature defoliation. Sporophores on the Leaf lesions were dominantly hypophyllous, rarely epiphyllous, solitary, erect, easily detachable, and as long as 750 μm. The upper portion of the sporophores consisted of a pyramidal head that was ventricose, 275 to 500 μm long, and 80 to 160 μm wide. The fungus was isolated from Leaf lesions and maintained on potato dextrose agar (PDA). Sclerotia were produced on PDA after 4 to 5 weeks at 18°C without ligh...

  • Zonate Leaf Spot of acer negundo caused by cristulariella moricola in korea
    Radiation Protection Dosimetry, 2012
    Co-Authors: Sungeun Cho, Ji Hyun Park, Sanghyun Lee, Hyang Burm Lee, Hyeon Dong Shin
    Abstract:

    In early August of 2011, following a prolonged period of cool and moist weather, several trees of boxelder maple (Acer negundo) planted in Mt. Soyo located in Dongducheon, Korea, were found to be heavily damaged by premature defoliation with Zonate Leaf Spot symptoms. Numerous number of cone-shaped, white sporophores (conidia) were observed on lesions of the abaxial Leaf surface. The morphological characteristics of conidia are of typical Cristulariella moricola, which was supported by analyzing sequences of internal transcribed spacer region of ribosomal DNA. Pathogenicity of the fungus was proved by artificial inoculation in the condition of relative humidity 100% and . This is the first report of the occurrence of Zonate Leaf Spot caused by infection of C. moricola on A. negundo in Korea.