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6-Monoacetylmorphine

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Simona Pichini – One of the best experts on this subject based on the ideXlab platform.

  • Development and validation of a gas chromatography-mass spectrometry assay for opiates and cocaine in human teeth.
    Journal of pharmaceutical and biomedical analysis, 2005
    Co-Authors: Manuela Pellegrini, Oscar Garcia-algar, Roberta Pacifici, Emilia Marchei, Adriana Casá, Ruth Mayné, Vanessa Barbero, Simona Pichini
    Abstract:

    Abstract A procedure based on gas chromatography–mass spectrometry (GC–MS) is described for determination of opiates (6-Monoacetylmorphine, morphine and codeine) and cocaine and metabolites (cocaine, benzoylecgonine and cocaethylene) in human teeth. After addition of nalorphine as internal standard, pulverized samples were incubated in HCl at 37 °C for 18 h. Then, after pH adjustment to 6, and the analytes were extracted with two volumes of 3 ml of chloroform/isopropanol (9:1). Chromatography was performed on a fused silisilica capillary column and analytes were determined in the selected-ion-monitoring (SIM) mode. The assay was validated in the range 7.5 (6.0 in case of codeine) to 500 ng/g with mean absolute recoveries ranged between 74.1 and 92.1% for the different analytes and precision and accuracy always better than 15%. The method was applied to the analysis of teeth from drug-addicts to assess past chronic consumption and verify self-reported declarations. In case of opiates, concentration range was 36.5–570.0 ng/g for 6-Monoacetylmorphine, 8.7–154.8 ng/g for morphine and 7.9–127.9 ng/g for codeine. Cocaine concentration ranged between 5.6 and 57.2 ng/g with its principal metabolite benzoylecgonine varying from 12.6 to 81.7 ng/g and cocaethylene present in only one sample at 10 ng/g value. Teeth can be a promising non-invasive biological matrix in biomedical analysis for both clinical and forensic purposes.

  • Development and validation of a liquid chromatography-mass spectrometry assay for the determination of opiates and cocaine in meconium.
    Journal of chromatography. B Analytical technologies in the biomedical and life sciences, 2003
    Co-Authors: Simona Pichini, Roberta Pacifici, Manuela Pellegrini, Emilia Marchei, E. Perez-alarcòn, C. Puig, Oriol Vall, Oscar Garcia-algar
    Abstract:

    A procedure based on liquid chrochromatography-mass spectrometry (LC-MS) is described for determination of 6-Monoacetylmorphine, morphine, morphine-3-glucuronide, morphine-6-glucuronide, codeine, cocaine, benzoylecgonine and cocaethylene in meconium using nalorfine as the internal standard. The analytes are initially extracted from the matrix by methanol (6-Monoacetylmorphine, morphine, codeine, cocaine, benzoylecgonine and cocaethylene) or 0.01 M ammonium hydrogen carbonate buffer (morphine-3-glucuronide, morphine-6-glucuronide). Subsequently a solid-phase extraction with Bondelut Certify columns (6-Monoacetylmorphine, morphine, codeine, cocaine, benzoylecgonine and cocaethylene) or ethyl solid-phase extraction columns (morphine-3-glucuronide, morphine-6-glucuronide) was applied. Chromatography was performed on a C(8) reversed-phase column using a gradient of acetic acid 1%-acetonitrile as a mobile phase. Analytes were determined in LC-MS single ion monitoring mode with atmospheric pressure ionisation-electrospray (ESI) interface. The method was validated in the range 0.005-1.00 microg/g using 1 g of meconium per assay and applied to analysis of meconium in newborns to assess fetal exposure to opiates and cocaine.

  • Analysis of opiates in human hair by high-performance liquid chromatography
    Journal of Liquid Chromatography & Related Technologies, 1999
    Co-Authors: Simona Pichini, Roberta Pacifici, Manuela Pellegrini, Ilaria Altieri, Piergiorgio Zuccaro
    Abstract:

    A high-performance liquid chromatographic method with ultraviolet radiation absorbance detection has been developed for the determination of the principal opiates (heroin, 6-Monoacetylmorphine, morphine, codeine) in human hair. An amount of 100 mg hair was incubated with 2 mL HCl 0.1 M at 56°C overnight and it was then extracted by solid-phase extraction using reversed-phase/ion exchange cartridges. Chromatography has been performed using a C18 reversed-phase column with a mobile phase consisting of water-acetonitrile (70:30 v/v) containing 0.01 M NaH2PO4 and 0.002 M sodium laurylsulphate at a final pH of 2.1. The linearity of the method was obtained in the concentration range of 0.5-50 ng/mg hair for 6-Monoacetylmorphine, morphine and codeine, and of 5–200 ng/mg hair for heroin. The accuracy of the methodology was assessed using reference material from National Institute of Standards and Technology consisting of human hair segments and powdered human hair soaked with opiates. Cocaine and benzoylecgonine

Ulrike Kohls – One of the best experts on this subject based on the ideXlab platform.

  • Body Fluids Using One Isolation Procedure and Liquid Chromatography-Atmospheric-Pressure Chemical-Ionization Mass Spectrometry*
    , 2016
    Co-Authors: Maciej Bogusz J. T, Rolf-dieter Maier, Klaus-dieter Kriiger, Ulrike Kohls
    Abstract:

    Abstract I A method for determining opiate agonists (morphine, morphine-3-glucuronide, morphine-6-glucuronide, 6-Monoacetylmorphine, codeine, codeine-6-glucuronide, dihydrocodeine, dihydromorphine, buprenorphine, methadone, tramadol, and ibogaine), cocaine and its metabolites (benzoylecgonine and ecgonine methyl ester) and lysergic acid diethylamide in serum, blood, urine and other biological matrices is presented. Aliquots (0.5-1.5 mL) of biological fluids were spiked with appropriate deuterated internal standards and extracted using a common solid-phase extraction method (Ct8 cartridges). The extracts were subjected to liquid chromatographic-atmospheric-pressure chemical-ionization mass spectrometric examination using selected ion monitoring procedures. These procedures were developed after analysis of full-scan mass spectra of examined compounds. The extraction method appeared very universal; the recoveries were high for almost all drugs and the extracts were very clean. The procedure was applied for routine forensic casework

  • Determination of Common Drugs of Abuse in Body Fluids Using One Isolation Procedure and Liquid Chromatography-Atmospheric-Pressure Chemical-Ionization Mass Spectrometry
    Journal of Analytical Toxicology, 1998
    Co-Authors: Maciej J. Bogusz, Rolf-dieter Maier, Klaus-dieter Krüger, Ulrike Kohls
    Abstract:

    I A method for determining opiate agonists (morphine, morphine-3-glucuronide, morphine-6-glucuronide, 6-Monoacetylmorphine, codeine, codeine-6-glucuronide, dihydrocodeine, dihydromorphine, buprenorphine, methadone, tramadol, and ibogaine), cocaine and its metabolites (benzoylecgonine and ecgonine methyl ester) and lysergic acid diethylamide in serum, blood, urine and other biological matrices is presented. Aliquots (0.5-1.5 mL) of biological fluids were spiked with appropriate deuterated internal standards and extracted using a common solid-phase extraction method (Ct8 cartridges). The extracts were subjected to liquid chromatographic-atmospheric- pressure chemical-ionization mass spectrometric examination using selected ion monitoring procedures. These procedures were developed after analysis of full-scan mass spectra of examined compounds. The extraction method appeared very universal; the

Manuela Pellegrini – One of the best experts on this subject based on the ideXlab platform.

  • Development and validation of a gas chromatography-mass spectrometry assay for opiates and cocaine in human teeth.
    Journal of pharmaceutical and biomedical analysis, 2005
    Co-Authors: Manuela Pellegrini, Oscar Garcia-algar, Roberta Pacifici, Emilia Marchei, Adriana Casá, Ruth Mayné, Vanessa Barbero, Simona Pichini
    Abstract:

    Abstract A procedure based on gas chromatography–mass spectrometry (GC–MS) is described for determination of opiates (6-Monoacetylmorphine, morphine and codeine) and cocaine and metabolites (cocaine, benzoylecgonine and cocaethylene) in human teeth. After addition of nalorphine as internal standard, pulverized samples were incubated in HCl at 37 °C for 18 h. Then, after pH adjustment to 6, and the analytes were extracted with two volumes of 3 ml of chloroform/isopropanol (9:1). Chromatography was performed on a fused silica capillary column and analytes were determined in the selected-ion-monitoring (SIM) mode. The assay was validated in the range 7.5 (6.0 in case of codeine) to 500 ng/g with mean absolute recoveries ranged between 74.1 and 92.1% for the different analytes and precision and accuracy always better than 15%. The method was applied to the analysis of teeth from drug-addicts to assess past chronic consumption and verify self-reported declarations. In case of opiates, concentration range was 36.5–570.0 ng/g for 6-Monoacetylmorphine, 8.7–154.8 ng/g for morphine and 7.9–127.9 ng/g for codeine. Cocaine concentration ranged between 5.6 and 57.2 ng/g with its principal metabolite benzoylecgonine varying from 12.6 to 81.7 ng/g and cocaethylene present in only one sample at 10 ng/g value. Teeth can be a promising non-invasive biological matrix in biomedical analysis for both clinical and forensic purposes.

  • Development and validation of a liquid chromatography-mass spectrometry assay for the determination of opiates and cocaine in meconium.
    Journal of chromatography. B Analytical technologies in the biomedical and life sciences, 2003
    Co-Authors: Simona Pichini, Roberta Pacifici, Manuela Pellegrini, Emilia Marchei, E. Perez-alarcòn, C. Puig, Oriol Vall, Oscar Garcia-algar
    Abstract:

    A procedure based on liquid chromatography-mass spectrometry (LC-MS) is described for determination of 6-Monoacetylmorphine, morphine, morphine-3-glucuronide, morphine-6-glucuronide, codeine, cocaine, benzoylecgonine and cocaethylene in meconium using nalorfine as the internal standard. The analytes are initially extracted from the matrix by methanol (6-Monoacetylmorphine, morphine, codeine, cocaine, benzoylecgonine and cocaethylene) or 0.01 M ammonium hydrogen carbonate buffer (morphine-3-glucuronide, morphine-6-glucuronide). Subsequently a solid-phase extraction with Bondelut Certify columns (6-Monoacetylmorphine, morphine, codeine, cocaine, benzoylecgonine and cocaethylene) or ethyl solid-phase extraction columns (morphine-3-glucuronide, morphine-6-glucuronide) was applied. Chromatography was performed on a C(8) reversed-phase column using a gradient of acetic acid 1%-acetonitrile as a mobile phase. Analytes were determined in LC-MS single ion monitoring mode with atmospheric pressure ionisation-electrospray (ESI) interface. The method was validated in the range 0.005-1.00 microg/g using 1 g of meconium per assay and applied to analysis of meconium in newborns to assess fetal exposure to opiates and cocaine.

  • Analysis of opiates in human hair by high-performance liquid chromatography
    Journal of Liquid Chromatography & Related Technologies, 1999
    Co-Authors: Simona Pichini, Roberta Pacifici, Manuela Pellegrini, Ilaria Altieri, Piergiorgio Zuccaro
    Abstract:

    A high-performance liquid chromatographic method with ultraviolet radiation absorbance detection has been developed for the determination of the principal opiates (heroin, 6-Monoacetylmorphine, morphine, codeine) in human hair. An amount of 100 mg hair was incubated with 2 mL HCl 0.1 M at 56°C overnight and it was then extracted by solid-phase extraction using reversed-phase/ion exchange cartridges. Chromatography has been performed using a C18 reversed-phase column with a mobile phase consisting of water-acetonitrile (70:30 v/v) containing 0.01 M NaH2PO4 and 0.002 M sodium laurylsulphate at a final pH of 2.1. The linearity of the method was obtained in the concentration range of 0.5-50 ng/mg hair for 6-Monoacetylmorphine, morphine and codeine, and of 5–200 ng/mg hair for heroin. The accuracy of the methodology was assessed using reference material from National Institute of Standards and Technology consisting of human hair segments and powdered human hair soaked with opiates. Cocaine and benzoylecgonine …