The Experts below are selected from a list of 4230 Experts worldwide ranked by ideXlab platform
Rui Tang - One of the best experts on this subject based on the ideXlab platform.
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carbon nanotubes as vegf carriers to improve the early vascularization of porcine small intestinal submucosa in Abdominal Wall Defect repair
International Journal of Nanomedicine, 2014Co-Authors: Zhengni Liu, Xueyi Feng, Huichun Wang, Wei Liu, Daxiang Cui, Rui TangAbstract:Insufficient early vascularization in biological meshes, resulting in limited host tissue incorporation, is thought to be the primary cause for the failure of Abdominal Wall Defect repair after implantation. The sustained release of exogenous angiogenic factors from a biocompatible nanomaterial might be a way to overcome this limitation. In the study reported here, multiWalled carbon nanotubes (MWNT) were functionalized by plasma polymerization to deliver vascular endothelial growth factor165 (VEGF165). The novel VEGF165-controlled released system was incorporated into porcine small intestinal submucosa (PSIS) to construct a composite scaffold. Scaffolds incorporating varying amounts of VEGF165-loaded functionalized MWNT were characterized in vitro. At 5 weight percent MWNT, the scaffolds exhibited optimal properties and were implanted in rats to repair Abdominal Wall Defects. PSIS scaffolds incorporating VEGF165-loaded MWNT (VEGF–MWNT–PSIS) contributed to early vascularization from 2–12 weeks postimplantation and obtained more effective collagen deposition and exhibited improved tensile strength at 24 weeks postimplantation compared to PSIS or PSIS scaffolds, incorporating MWNT without VEGF165 loading (MWNT–PSIS).
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reconstruction of Abdominal Wall musculofascial Defects with small intestinal submucosa scaffolds seeded with tenocytes in rats
Tissue Engineering Part A, 2013Co-Authors: Zhicheng Song, Zhiyou Peng, Zhengni Liu, Jianjun Yang, Rui TangAbstract:The repair of Abdominal Wall Defects following surgery remains a difficult challenge. Although multiple methods have been described to restore the integrity of the Abdominal Wall, there is no clear consensus on the ideal material for reconstruction. This study explored the feasibility of in vivo reconstruction of a rat model of an Abdominal Wall Defect with a composite scaffold of tenocytes and porcine small intestinal submucosa (SIS). In the current study, we created a 2×1.5 cm Abdominal Wall Defect in the anterolateral Abdominal Wall of Sprague-Dawley rats, which were assigned into three groups: the cell-SIS construct group, the cell-free SIS scaffold group, and the Abdominal Wall Defect group. Tenocytes were obtained from the tendons of rat limbs. After isolation and expansion, cells (2×107/mL) were seeded onto the three-layer SIS scaffolds and cultured in vitro for 5 days. Cell-SIS constructs or cell-free constructs were implanted to repair the Abdominal Wall Defects. The results showed that the tenoc...
Zhengni Liu - One of the best experts on this subject based on the ideXlab platform.
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carbon nanotubes as vegf carriers to improve the early vascularization of porcine small intestinal submucosa in Abdominal Wall Defect repair
International Journal of Nanomedicine, 2014Co-Authors: Zhengni Liu, Xueyi Feng, Huichun Wang, Wei Liu, Daxiang Cui, Rui TangAbstract:Insufficient early vascularization in biological meshes, resulting in limited host tissue incorporation, is thought to be the primary cause for the failure of Abdominal Wall Defect repair after implantation. The sustained release of exogenous angiogenic factors from a biocompatible nanomaterial might be a way to overcome this limitation. In the study reported here, multiWalled carbon nanotubes (MWNT) were functionalized by plasma polymerization to deliver vascular endothelial growth factor165 (VEGF165). The novel VEGF165-controlled released system was incorporated into porcine small intestinal submucosa (PSIS) to construct a composite scaffold. Scaffolds incorporating varying amounts of VEGF165-loaded functionalized MWNT were characterized in vitro. At 5 weight percent MWNT, the scaffolds exhibited optimal properties and were implanted in rats to repair Abdominal Wall Defects. PSIS scaffolds incorporating VEGF165-loaded MWNT (VEGF–MWNT–PSIS) contributed to early vascularization from 2–12 weeks postimplantation and obtained more effective collagen deposition and exhibited improved tensile strength at 24 weeks postimplantation compared to PSIS or PSIS scaffolds, incorporating MWNT without VEGF165 loading (MWNT–PSIS).
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reconstruction of Abdominal Wall musculofascial Defects with small intestinal submucosa scaffolds seeded with tenocytes in rats
Tissue Engineering Part A, 2013Co-Authors: Zhicheng Song, Zhiyou Peng, Zhengni Liu, Jianjun Yang, Rui TangAbstract:The repair of Abdominal Wall Defects following surgery remains a difficult challenge. Although multiple methods have been described to restore the integrity of the Abdominal Wall, there is no clear consensus on the ideal material for reconstruction. This study explored the feasibility of in vivo reconstruction of a rat model of an Abdominal Wall Defect with a composite scaffold of tenocytes and porcine small intestinal submucosa (SIS). In the current study, we created a 2×1.5 cm Abdominal Wall Defect in the anterolateral Abdominal Wall of Sprague-Dawley rats, which were assigned into three groups: the cell-SIS construct group, the cell-free SIS scaffold group, and the Abdominal Wall Defect group. Tenocytes were obtained from the tendons of rat limbs. After isolation and expansion, cells (2×107/mL) were seeded onto the three-layer SIS scaffolds and cultured in vitro for 5 days. Cell-SIS constructs or cell-free constructs were implanted to repair the Abdominal Wall Defects. The results showed that the tenoc...
Michel Saintcyr - One of the best experts on this subject based on the ideXlab platform.
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use of a pre expanded propeller deep inferior epigastric perforator diep flap for a large Abdominal Wall Defect
Journal of Plastic Reconstructive and Aesthetic Surgery, 2013Co-Authors: Angela Cheng, Michel SaintcyrAbstract:Large Abdominal Wall soft tissue Defects are traditionally reconstructed using loco-regional flaps and/or tissue expanders. Pedicled perforator flaps offer similar tissue coverage with minimal donor site morbidity and do not require microsurgical skills. The deep inferior epigastric perforator (DIEP) flap has become increasingly popular for breast reconstruction and offers abundant soft tissue while permitting primary donor site closure. To minimize operative sessions, recovery time, donor site morbidity, and avoid microsurgery, we combined tissue expansion with a "propeller" flap design and report this first case of a pre-expanded pedicled perforator flap based on the DIEP to address a large Abdominal Wall Defect due to a burn injury.
Jong Bum Kwon - One of the best experts on this subject based on the ideXlab platform.
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newly developed porcine acellular dermal matrix xenoderm for adhesion prevention and rreconstruction of aAbdominal Wall Defect in rat
Tissue Engineering and Regenerative Medicine, 2013Co-Authors: Seok Bae Oh, Jong Bum KwonAbstract:Various prosthetic materials have been employed to reconstruct Abdominal Wall Defects. Prosthetic materials have been used widely to repair the Abdominal Defects; however, complications with mesh infection and adhesion have led to the recent use of more biocompatible implants derived from animal or human sources for the surgical repair of Abdominal Wall Defects. The purpose of the present study was to evaluate the effectiveness of the newly developed acellular porcine dermis (XenoDerm) to prevent adhesion in the reconstruction of Abdominal Wall Defects compared with human acellular dermal matrix (SureDerm™) in a rat model. Forty adult Sprague-Dawley rats underwent repair of surgically created ventral hernias using porcine, acellular dermal-matrix or human acellular dermal-matrix. The grade of adhesion (peritoneal adhesions by Hooker score and intra-Abdominal adhesions by Knightly score), histologic analysis (using hematoxylin-eosin stain, Masson-trichrome stain and immunohistochemical stain) and tensile strength (by MultiTest 1-i tensiometer) were assessed, after the animals were euthanized at 1 week and at 4 week postoperatively. At 4 week after the repair of Abdominal Wall Defect, the porcine acellular dermal matrix incorporated into the host tissue and revealed no significant difference in adhesion, histologic analysis, or tensile strength compared with the human acellular dermal matrix. Based on these results, we conclude that acellular porcine dermal matrix (XenoDerm) is useful for Abdominal Wall reconstruction. However, subsequent studies with longer time periods and clinical studies will be needed to reveal the consequences of its use in Abdominal Wall Defect reconstruction.
Kathleen A Derwin - One of the best experts on this subject based on the ideXlab platform.
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investigating muscle regeneration with a dermis small intestinal submucosa scaffold in a rat full thickness Abdominal Wall Defect model
Journal of Biomedical Materials Research Part B, 2015Co-Authors: Sambit Sahoo, Andrew R Baker, Kathleen A DerwinAbstract:Repair of large complex ventral Wall hernias is challenging and outcomes are often poor due to hernia recurrence and compromised Abdominal Wall function. Currently, biological grafts are used to assist in repairing these complex hernias. Dermis grafts are often chosen because of their mechanical characteristics; however, dermis does not have the ability to promote the muscle regeneration needed to regain Abdominal Wall function. In contrast, small intestinal submucosa (SIS) grafts have been shown to promote muscle generation in volumetric muscle loss (VML) models. Hence, the objective of this study is to investigate the extent to which SIS grafts can be used together with dermis grafts to repair and promote muscle regeneration in a full-thickness Abdominal Wall Defect in a rat model. The dermis layer is intended to mechanically bridge the Defect and support constructive tissue remodeling while the SIS is intended to degrade and promote neo-muscle formation. After 16 weeks of implantation, we found only a small amount of vascularized muscle (<10% of the Defect area) in the repaired Defects. No significant difference in Defect muscle area was found between the groups receiving the dermis + SIS scaffolds and the control (dermis alone) group. Our findings indicate that the SIS constructions investigated could not promote appreciable muscle regeneration in this rigorous animal model of VML and incomplete Abdominal closure. Future investigation into combination scaffold, cell and molecular therapies would be warranted to address the need for functional muscle regeneration in challenging clinical conditions such as complex Abdominal Wall repair. © 2014 Wiley Periodicals, Inc. J Biomed Mater Res Part B: Appl Biomater, 103B: 355–364, 2015.
