The Experts below are selected from a list of 273 Experts worldwide ranked by ideXlab platform
Kerstin Voigt - One of the best experts on this subject based on the ideXlab platform.
-
Species Recognition and Clinical Relevance of the Zygomycetous Genus Lichtheimia (syn. Absidia Pro Parte, Mycocladus)
Journal of clinical microbiology, 2010Co-Authors: Ana Alastruey-izquierdo, Kerstin Hoffmann, Kerstin Voigt, G.s. De Hoog, J. L. Rodriguez-tudela, E. Bibashi, Grit WaltherAbstract:The zygomycete genus Lichtheimia (syn. Absidia pro parte, Mycocladus) consists of saprotrophic fungi inhabiting soil or dead plant material. Lichtheimia corymbifera (syn. Absidia corymbifera, Mycocladus corymbifer) and Lichtheimia ramosa (syn. Absidia ramosa, Mycocladus ramosus) may cause fulminant infections in patients with impaired immunity. The present study investigated the species boundaries in Lichtheimia using genealogical concordance phylogenetic species recognition (by comparison of the genealogies of the internal transcribed spacer [ITS] sequence, the D1/D2 region of the large subunit [LSU], and actin), biological species recognition by mating tests, as well as morphological and physiological characteristics. The three molecular markers used were selected by evaluating the polymorphisms and paralogies of several loci, including those for β-tubulin, translation elongation factor 1α, the two largest subunits of the RNA polymerase II (RPB1 and RPB2), the mitochondrial cytochrome c oxidase subunit I (COI), and the mitochondrial small-subunit (mtSSU) rDNA, among four strains belonging to different putative species. Comparing the genealogies of the ITS, LSU, and actin genes, we recognized seven phylogenetic species. However, mating tests did not show intrinsic reproductive barriers for two pairs of the phylogenetic species. Therefore, we regard five species in Lichtheima to be confirmed: Lichtheimia corymbifera, L. ornata comb. nov., L. ramosa, L. hyalospora, and L. sphaerocystis sp. nov. Only the first three species seem to have clinical relevance. Lichtheimia blakesleeana is reduced to a synonym of Lichtheimia hyalospora. We provide a detailed description of Lichtheimia sphaerocystis sp. nov. and a key for the identification of all accepted species identified in the present study on the basis of their morphological traits and growth at different temperatures.
-
revision of the genus Absidia mucorales zygomycetes based on physiological phylogenetic and morphological characters thermotolerant Absidia spp form a coherent group mycocladiaceae fam nov
Fungal Biology, 2007Co-Authors: Kerstin Hoffmann, Sabrina Discher, Kerstin VoigtAbstract:The genus Absidia comprises ubiquitously distributed soil fungi inhabiting different growth temperature optima ranging from 20-42 degrees C. Some of the mesophilic species are important biotechnologically in the biotransformation of steroids or as producers of rennin-like components, whereas species with higher growth temperature optima are of clinical relevance as opportunistic human pathogens. The aim of this study was to investigate the phylogenetic relationships between these species and to establish a revision of their systematics. For this purpose single and combined genealogies based on distance, MP, ML, and Bayesian analyses of aligned nucleotide sequences of the nuclear-encoded genes for actin (act) and for the 5.8S ribosomal RNA flanked by the ITS regions 1 and 2 (comprising 807 and 828 characters, respectively) of 16 Absidia species were reconstructed. The phylogenetic reconstructions suggest a trichotomy of the Absidia genus consisting of a mesophilic, a fast-growing thermotolerant, and a slowly-growing mycoparasitic Absidia group. The trichotomous phylogenetic grouping is concordant with the morphology of the zygospores, which are zygotes resulting from sexual conjugation between two compatible mating partners. Whereas the mesophilic group comprises the majority of Absidiaceaeous species forming sterile hair-like, mycelial appendages on the suspensors of their zygospores, the thermotolerant group is characterised by the formation of smooth-walled zygospores, and the mycoparasitic group, namely Absidia parricida and A. zychae, by Mucor-like rough-walled zygospores. Based on the phylogenetic coherence of mesophilic and thermotolerant Absidia species, we propose that the two groups are separated into two distinct genera, Absidia for the mesophilic Absidia species resembling the Absidiaceae and Mycocladus for the thermotolerant species A. corymbifera, A. blakesleeana and A. hyalospora. Because Mycocladus is physiologically, phylogenetically, and morphologically distinct from the Absidiaceae s. str. we suggest that they are classified as a separate family, Mycocladiaceae fam. nov., which comprises the three species M. corymbifer, M. blakesleeanus and M. hyalospora.
Kerstin Hoffmann - One of the best experts on this subject based on the ideXlab platform.
-
Species Recognition and Clinical Relevance of the Zygomycetous Genus Lichtheimia (syn. Absidia Pro Parte, Mycocladus)
Journal of clinical microbiology, 2010Co-Authors: Ana Alastruey-izquierdo, Kerstin Hoffmann, Kerstin Voigt, G.s. De Hoog, J. L. Rodriguez-tudela, E. Bibashi, Grit WaltherAbstract:The zygomycete genus Lichtheimia (syn. Absidia pro parte, Mycocladus) consists of saprotrophic fungi inhabiting soil or dead plant material. Lichtheimia corymbifera (syn. Absidia corymbifera, Mycocladus corymbifer) and Lichtheimia ramosa (syn. Absidia ramosa, Mycocladus ramosus) may cause fulminant infections in patients with impaired immunity. The present study investigated the species boundaries in Lichtheimia using genealogical concordance phylogenetic species recognition (by comparison of the genealogies of the internal transcribed spacer [ITS] sequence, the D1/D2 region of the large subunit [LSU], and actin), biological species recognition by mating tests, as well as morphological and physiological characteristics. The three molecular markers used were selected by evaluating the polymorphisms and paralogies of several loci, including those for β-tubulin, translation elongation factor 1α, the two largest subunits of the RNA polymerase II (RPB1 and RPB2), the mitochondrial cytochrome c oxidase subunit I (COI), and the mitochondrial small-subunit (mtSSU) rDNA, among four strains belonging to different putative species. Comparing the genealogies of the ITS, LSU, and actin genes, we recognized seven phylogenetic species. However, mating tests did not show intrinsic reproductive barriers for two pairs of the phylogenetic species. Therefore, we regard five species in Lichtheima to be confirmed: Lichtheimia corymbifera, L. ornata comb. nov., L. ramosa, L. hyalospora, and L. sphaerocystis sp. nov. Only the first three species seem to have clinical relevance. Lichtheimia blakesleeana is reduced to a synonym of Lichtheimia hyalospora. We provide a detailed description of Lichtheimia sphaerocystis sp. nov. and a key for the identification of all accepted species identified in the present study on the basis of their morphological traits and growth at different temperatures.
-
identification of the genus Absidia mucorales zygomycetes a comprehensive taxonomic revision
2010Co-Authors: Kerstin HoffmannAbstract:This brief review intends to survey and evaluate the present knowledge about the genus Absidia Tiegh. sensu lato regarding the traditional and current position within the order Mucorales (Mucoromycotina, Zygomycetes), nomenclatural changes and the taxonomical rearrangements of the prevalent species. Species grouped within Absidia possess some very promising industrial and medical application possibilities e.g., as mediators of biotransformations, producers of antimicrobial and wound healing stimulators. But some species are also causatives of severe and frequent fatal mucormycoses. Using traditional and modern methods of species determination had uncovered a trichotomous genera separation, namely Absidia sensu stricto, Lichtheimia, and Lentamyces, belonging to distinct families, the Absidiaceae, Lichtheimiaceae and “Lentamycetaceae” (NN), respectively. The existing medical and industrial aspects necessitate a fast and secure identification of a prominent species. Within this survey, morphological criteria and molecular markers were proposed for clear distinction of Absidia sensu lato.
-
revision of the genus Absidia mucorales zygomycetes based on physiological phylogenetic and morphological characters thermotolerant Absidia spp form a coherent group mycocladiaceae fam nov
Fungal Biology, 2007Co-Authors: Kerstin Hoffmann, Sabrina Discher, Kerstin VoigtAbstract:The genus Absidia comprises ubiquitously distributed soil fungi inhabiting different growth temperature optima ranging from 20-42 degrees C. Some of the mesophilic species are important biotechnologically in the biotransformation of steroids or as producers of rennin-like components, whereas species with higher growth temperature optima are of clinical relevance as opportunistic human pathogens. The aim of this study was to investigate the phylogenetic relationships between these species and to establish a revision of their systematics. For this purpose single and combined genealogies based on distance, MP, ML, and Bayesian analyses of aligned nucleotide sequences of the nuclear-encoded genes for actin (act) and for the 5.8S ribosomal RNA flanked by the ITS regions 1 and 2 (comprising 807 and 828 characters, respectively) of 16 Absidia species were reconstructed. The phylogenetic reconstructions suggest a trichotomy of the Absidia genus consisting of a mesophilic, a fast-growing thermotolerant, and a slowly-growing mycoparasitic Absidia group. The trichotomous phylogenetic grouping is concordant with the morphology of the zygospores, which are zygotes resulting from sexual conjugation between two compatible mating partners. Whereas the mesophilic group comprises the majority of Absidiaceaeous species forming sterile hair-like, mycelial appendages on the suspensors of their zygospores, the thermotolerant group is characterised by the formation of smooth-walled zygospores, and the mycoparasitic group, namely Absidia parricida and A. zychae, by Mucor-like rough-walled zygospores. Based on the phylogenetic coherence of mesophilic and thermotolerant Absidia species, we propose that the two groups are separated into two distinct genera, Absidia for the mesophilic Absidia species resembling the Absidiaceae and Mycocladus for the thermotolerant species A. corymbifera, A. blakesleeana and A. hyalospora. Because Mycocladus is physiologically, phylogenetically, and morphologically distinct from the Absidiaceae s. str. we suggest that they are classified as a separate family, Mycocladiaceae fam. nov., which comprises the three species M. corymbifer, M. blakesleeanus and M. hyalospora.
Claudio Viscoli - One of the best experts on this subject based on the ideXlab platform.
-
Soft-tissue infection with Absidia corymbifera and kidney complications in an AIDS patient.
Medical mycology, 2007Co-Authors: Elda Righi, C. G. Giacomazzi, Matteo Bassetti, Francesca Bisio, O. Soro, J. L. Mcdermott, Oliviero E. Varnier, Sandra Ratto, Claudio ViscoliAbstract:We describe a case of primary cutaneous Absidia corymbifera infection in an AIDS patient with renal complications. The Sensititre YeastOne panel was adopted to determine antifungal susceptibility and liposomial amphotericin B was used which initially produced a significant clinical response.
Hadi, Duaa Hassan - One of the best experts on this subject based on the ideXlab platform.
-
The Effect of Trigonella foemun-gracum Extract on the Fungi that Isolated from the Libraries Door
'University of Babylon - Physical Education and Sports Sciences', 2019Co-Authors: Hadi, Duaa HassanAbstract:تضمنت الدراسة عزل وتشخيص الفطريات من ابواب المختبرات (الاحياء المجهرية،االتقنية الاحيائية) في المعهد التقني –بابل للأقسام الطبية.اذ اظهرت الدراسة عددا من الانواع الفطرية : (Absidia sp,Penicillium spp,Candida sp,Aspergillus). وكانت نسبة ظهور الانواع الفطرية في ابواب مختبرات الاحياء المجهرية اعلى من ابواب المختبرات التقنية الاحيائية ، اذ سجل الفطر Aspergillus niger نسبة 36,8% و Absidia ،Penicillium نسبة 21,05% و Candida sp نسبة 15,7% و Aspergillus flavus نسبة 5,2% في حين ابواب المختبرات التقنية الاحيائية سجل الفطر Aspergillus terrus نسبة15,7 وAspergillus niger’ ,Candida albicans Penicillium نسبة 20% وAbsidia ,Alternaria نسبة 10%. وقد اظهرت دراسة التاثير المثبط للمستخلص المائي الحار لنبات الحلبة وبالتراكيز المستعملة (40،30،20،10) ملغم /مل على بعض الفطريات المعزولة . نسبة تثبيط 100% بالنسبة للفطر Candida albicans في التراكيز (30،40) ملغم /مل ومعدل قطر المستعمرة الخميرة بتركيز 30% ملغم /مل كان (1) سم اما الفطر Aspergillus niger في التراكيز (20،10) ملغم /مل لم يعط اي مقاومة تجاه المستخلص.The study included the isolation and diagnosis of the fungi from the door of the laboratories (microbology ,technical biology )in the technical institute of Babylon from the medical department. The study appeared many of the different kinds of fungi: Absidia sp,Candidia sp, Aspergillus sp,Penicillum sp. The rate of appearance of the type of the fungi in microbology laboratory is the higher than the technical biology laboratory other.The rate of appearance of the fungi Aspergillus niger was 36.8% and Absidia ,Penicililum was 21.05% , Candida sp was 15.7 % , Aspergillus flavus 5.2% while in the technical biology the fungi Aspergillus terrus was 15.7% and the fungi Penicililum sp,Candida albcans, Apergillus niger was 20% , Absidia sp ,Alternaria sp was 10%.The study showed the influence of the heating water extract hot watering extract the race track in the concentration which is used( 10,20,30, 40) mg /ml on some of fungi. The rate of reduction is appeared 100% of the fungi Candida albicans in the concentration (30,40) mg/ml and the range of the diameter colony of the yeast Candida albicans in the concentration 30 mg/ml the (1)sanatamtar. While the fungi Aspergillus niger in the concentration (10,20)mg/ml is not given any resistance toward the extract.
-
The Effect of Nigella sative Extract on the Fungi that Isolated from Desktop's of the Tables
'University of Babylon', 2018Co-Authors: Hadi, Duaa HassanAbstract:تضمنت الدراسة عزل وتشخيص الفطريات من طاولات سطح المكتب (غرف الموظفين، المختبرات الطبية). اذا اظهرت الدراسة عددا من الانواع الفطرية : ( Alternaria sp ,Absidia sp,Candidia sp,Aspergillus sp,Penicillum sp).وكانت نسبة ظهور الانواع الفطرية في طاولات سطح المكتب غرف الموظفين اعلى من طاولات سطح المكتب للمختبرات الطبية ,حيث سجل الفطر sp Candidia نسبة ظهورة 36.8% وAspergillus niger,Penicillum sp21.05 % وAbsidia sp %15.7و%5.2 Aspergillus flavus في حين طاولات سطح مكتب المختبرات الطبية سجل الفطرAspergillus terrus 15.7% والفطر Absidia sp ,Candidia Apergillus niger 20% وsp Penicillum sp ,Alternaria 10% . وقد كانت نتائج لكشف في المواد الفعالة هي (التامين والكلايكوسيد والقلويات والزيوت الطيارة ) نتيجة موجبة وPh 5 .وقد اظهرت دراسة التأثير اقطار منطقة التثبيط للمستخلص المائي الحار لنبات الحبة السوداء) حبة البركة) وبالتراكيز المستعملة (10،30،20،40) ملغم /مل على بعض الفطريات المعزولة. نسبة قطر منطقة التثبيط 100% بالنسبة للفطر sp Penicillum في التراكيز (40،30) ملغم /مل ومعدل قطر التثبيط الخميرة Candidia albicansبتركيز 30 ملغم /مل كان (1)ملم ،اما الفطر Aspergillus niger في التراكيز (20،10) ملغم /مل قد اعطى مقاومة تجاه المستخلص هو 2. The study included the isolation and diagnosis of the fungi from the Desktop's of the tables (instructers ,medical laboratories). The study appeared many of the different kinds of fungi:(Absidia sp,Candidia sp, Aspergillus sp,Penicillum sp and Alternaria sp) The rate of appearance of the type of the fungi in Desktop's of the tables instructers is the higher than the Desktop's of the tables of the medical laboratories . The rate of appearance of the fungi Candidia is recoded 36.8% and Penicillum sp,Aspergillus niger is recoded 21.05% and Aspergillus flavus recoded 5.2 %, Abisidia sp recoded 15.7%. While in the Desktop's of the tables medical laboratories the fungi Aspergillus terrus is recorded 15.7% and the fungi Absidia ,Candidia, Apergillus niger is recoded 20% , Penicillum sp ,Alternaria sp is recoded 10%. The study showed the influence of the hot water extract of the Nigella sative the concentration which is used(10,20,30, 40) mg /ml on some of fungi. The rate of reduction is appeared 100% of the fungi Penicillum sp in the concentration (30,40) mg/ml and the range of the diameter colony of the yeast Candidia albicanis in the concentration 30 mg/ml the (1)cm. While the fungi Aspergillus niger in the concentration (10,20)mg/ml is given resistance toward the extract 2
-
The Effect of Trigonella foemun-gracum Extract on the Fungi that Isolated from Cleaning Powder (Soap)
'University of Babylon - Physical Education and Sports Sciences', 2018Co-Authors: Hadi, Duaa HassanAbstract:تضمنت الدراسة عزل وتشخيص الفطريات من مساحيق التنظيف في الصابون (مغاسل الموظفين، مغاسل الطلاب). اذا اظهرت الدراسة عددا من الانواع الفطرية :(Absidia sp,Candidia sp, Aspergillus sp,Penicillum sp).وكانت نسبة ظهور الانواع الفطرية في مغاسل الموظفين اعلى من مغاسل الطلاب ,حيث سجل الفطر sp Absidiaنسبة ظهورة 36.8% وAspergillus niger ,Candidia 21.05% و % 5.2 Aspergillus flavus %15.7Penicillum sp في حين المغاسل الطلاب سجل الفطر Aspergillus sp 15.7% والفطر Absidia sp ,Candidia Apergillus terrus 20% وsp Penicillum sp ,Alternaria 10% وقد اظهرت دراسة التأثير المثبط للمستخلص المائي الحار لنبات الحلبة وبالتراكيز المستعملة (10،30،20،40)ملغم /مل على بعض الفطريات المعزولة. نسبة تثبيط 100% بالنسبة للفطر sp Absidia في التراكيز (40،30) ملغم /مل ومعدل قطر المستعمرة الخميرة Candidia albicansبتركيز 30 ملغم /مل كان (1)سم ،اما الفطر Aspergillus niger في التراكيز (20،10) ملغم /مل لم يعطي اي مقاومة تجاه المستخلص.The study included the isolation and diagnosis of the fungi from the cleaning powder in the soap(Laundries' staff, Laundries of student). The study appeared many of the different kinds of fungi:( Absidia sp,Candidia sp, Aspergillus sp,Penicillum sp). The rate of appearance of the type of the fungi in Laundries ' staff is the higher than the laundries' of the students.The rate of appearance of the fungi Absidia is recoded 36.8% and Candidia ,Aspergillus niger is recoded 21.05% and Aspergillus flavus recoded 5.2 % , Penicillum sp recoded 15.7% while in the laundries of students the fungi Aspergillus niger is recorded 15.7% and the fungi Absidia ,Candidia, Apergillus terrus is recoded 20% , Penicillum sp ,Alternaria sp is recoded 10%.The study showed the influence of the heating water extract hot watering extract the race track in the concentration which is used( 10,20,30, 40) mg /ml on some of fungi. The rate of reduction is appeared 100% of the fungi Absidia in the concentration (30,40) mg/ml and the range of the diameter colony of the yeast Candidia albicanis in the concentration 30 mg/ml the (1)sanatamtar. While the fungi Aspergillus niger in the concentration (10,20)mg/ml is not given any resistance toward the extract
Jacques Florence - One of the best experts on this subject based on the ideXlab platform.
-
(1949) Proposal to conserve Tieghemella Pierre (Sapotaceae) against Tieghemella Berl. & De Toni (Absidiaceae)
Taxon, 2010Co-Authors: Arnaud Mouly, Laurent Gautier, Joëlle Dupont, Hervé Chevillotte, Jean-louis Guillaumet, Jacques FlorenceAbstract:Tieghemella Pierre, Not. Bot. 1: 18. 30 Dec 1890 [Sapot.], nom. cons. prop. Typus: T. africana Pierre. Tieghemella Berl. & De Toni in Saccardo, Syll. Fungorum 7: 215. 1888 [Fungi], nom. rej. prop. Typus: T. repens (Tiegh.) Berl. & De Toni (Absidia repens Tiegh.).
