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Sang Soo Sim - One of the best experts on this subject based on the ideXlab platform.
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inhibitory effect of Acteoside on melittin induced catecholamine exocytosis through inhibition of ca2 dependent phospholipase a2 and extracellular ca2 influx in pc12 cells
Archives of Pharmacal Research, 2015Co-Authors: Ho Sun Song, Wan Kyunn Whang, Sang Soo SimAbstract:To investigate the inhibitory effect of Acteoside on the process of exocytosis induced by melittin, we measured Ca2+ mobilization, arachidonic acid (AA) release and catecholamine exocytosis in PC12 chromaffin cells. Melittin significantly increased the intracellular Ca2+ mobilization via receptor-operated calcium channel but not the intracellular Ca2+ release. It caused AA release via activation of Ca2+-dependent phospholipase A2 (PLA2) and catecholamine secretion in a dose-dependent manner. Acteoside dose-dependently inhibited the release of AA and intracellular Ca2+ mobilization induced by melittin. Acteoside reduced the catecholamine release and raised the amount of intracellular chromogranin A which is co-released with catecholamine from melittin-stimulated PC12 cells. Taken together, our results suggest that Acteoside could suppress the exocytosis via inhibition of Ca2+-dependent PLA2 and extracellular Ca2+ influx in PC12 cells stimulated by melittin.
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competitive inhibition of cytosolic ca2 dependent phospholipase a2 by Acteoside in rbl 2h3 cells
Archives of Pharmacal Research, 2012Co-Authors: Ho Sun Song, Wan Kyunn Whang, Mi Young Choi, Jae Min Jeong, Yong Ho Kim, Beom Hyeon Jang, Ji Hoon Sung, Min Gyu Kim, Sang Soo SimAbstract:The aim of this study was to investigate whether Acteoside isolated from Clerodendron trichotomum Thunberg may act as a selective inhibitor of phospholipase A(2) in RBL-2H3 cells. Acteoside dose-dependently inhibited 0.5 μM melittin-induced release of [(3)H]arachidonic acid, which was due to the inhibition of cytosolic Ca(2+)-dependent phospholipase A(2) (cPLA(2)) rather than secretory PLA(2) (sPLA(2)). In Dixon plots, the apparent K ( i ) value of Acteoside on cPLA(2) was 5.9 μM and the inhibitory pattern appeared to be a competitive inhibitor. The above data, suggests that Acteoside acts as a competitive inhibitor of cPLA(2) in RBL-2H3 cells.
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the effect of Acteoside on histamine release and arachidonic acid release in rbl 2h3 mast cells
Archives of Pharmacal Research, 2006Co-Authors: Jin Hee Lee, Ji Yun Lee, Hyo Suk Kang, Chan Hun Jeong, Hee Moon, Wan Kyunn Whang, Chang Jong Kim, Sang Soo SimAbstract:The effect of Acteoside, a phenylpropanoid glycoside isolated fromClerodendron trichotomum Thunberg, on histamine and arachidonic acid release was investigated in RBL 2H3 cells. Histamine was dose-dependently released from RBL 2H3 cells by melittin, arachidonic acid and thapsigargin. In extracellular Ca2+-free solution, basal secretion of histamine increased by two fold. The response of histamine release to melittin and thapsigargin in Ca2+-free solution was significantly decreased, whereas the response to arachidonic acid was significantly increased as compared with those in normal solution. Acteoside inhibited histamine release induced by melittin, arachidonic acid and thapsigargin in a dose-dependent manner in the presence or absence of extracellular Ca2+. However, the inhibitory activity of Acteoside was more potent in normal solution than that in Ca2+-free solution. These data suggest that inhibitory mechanism of Acteoside on histamine release may be related to extracellular Ca2+. On the other hand, Acteoside significantly inhibited arachidonic acid release and prostaglandin E2 production induced by 0.5 μM melittin. It is possible that Acteoside may be developed as an anti-inflammatory agent.
Wan Kyunn Whang - One of the best experts on this subject based on the ideXlab platform.
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inhibitory effect of Acteoside on melittin induced catecholamine exocytosis through inhibition of ca2 dependent phospholipase a2 and extracellular ca2 influx in pc12 cells
Archives of Pharmacal Research, 2015Co-Authors: Ho Sun Song, Wan Kyunn Whang, Sang Soo SimAbstract:To investigate the inhibitory effect of Acteoside on the process of exocytosis induced by melittin, we measured Ca2+ mobilization, arachidonic acid (AA) release and catecholamine exocytosis in PC12 chromaffin cells. Melittin significantly increased the intracellular Ca2+ mobilization via receptor-operated calcium channel but not the intracellular Ca2+ release. It caused AA release via activation of Ca2+-dependent phospholipase A2 (PLA2) and catecholamine secretion in a dose-dependent manner. Acteoside dose-dependently inhibited the release of AA and intracellular Ca2+ mobilization induced by melittin. Acteoside reduced the catecholamine release and raised the amount of intracellular chromogranin A which is co-released with catecholamine from melittin-stimulated PC12 cells. Taken together, our results suggest that Acteoside could suppress the exocytosis via inhibition of Ca2+-dependent PLA2 and extracellular Ca2+ influx in PC12 cells stimulated by melittin.
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competitive inhibition of cytosolic ca2 dependent phospholipase a2 by Acteoside in rbl 2h3 cells
Archives of Pharmacal Research, 2012Co-Authors: Ho Sun Song, Wan Kyunn Whang, Mi Young Choi, Jae Min Jeong, Yong Ho Kim, Beom Hyeon Jang, Ji Hoon Sung, Min Gyu Kim, Sang Soo SimAbstract:The aim of this study was to investigate whether Acteoside isolated from Clerodendron trichotomum Thunberg may act as a selective inhibitor of phospholipase A(2) in RBL-2H3 cells. Acteoside dose-dependently inhibited 0.5 μM melittin-induced release of [(3)H]arachidonic acid, which was due to the inhibition of cytosolic Ca(2+)-dependent phospholipase A(2) (cPLA(2)) rather than secretory PLA(2) (sPLA(2)). In Dixon plots, the apparent K ( i ) value of Acteoside on cPLA(2) was 5.9 μM and the inhibitory pattern appeared to be a competitive inhibitor. The above data, suggests that Acteoside acts as a competitive inhibitor of cPLA(2) in RBL-2H3 cells.
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the effect of Acteoside on histamine release and arachidonic acid release in rbl 2h3 mast cells
Archives of Pharmacal Research, 2006Co-Authors: Jin Hee Lee, Ji Yun Lee, Hyo Suk Kang, Chan Hun Jeong, Hee Moon, Wan Kyunn Whang, Chang Jong Kim, Sang Soo SimAbstract:The effect of Acteoside, a phenylpropanoid glycoside isolated fromClerodendron trichotomum Thunberg, on histamine and arachidonic acid release was investigated in RBL 2H3 cells. Histamine was dose-dependently released from RBL 2H3 cells by melittin, arachidonic acid and thapsigargin. In extracellular Ca2+-free solution, basal secretion of histamine increased by two fold. The response of histamine release to melittin and thapsigargin in Ca2+-free solution was significantly decreased, whereas the response to arachidonic acid was significantly increased as compared with those in normal solution. Acteoside inhibited histamine release induced by melittin, arachidonic acid and thapsigargin in a dose-dependent manner in the presence or absence of extracellular Ca2+. However, the inhibitory activity of Acteoside was more potent in normal solution than that in Ca2+-free solution. These data suggest that inhibitory mechanism of Acteoside on histamine release may be related to extracellular Ca2+. On the other hand, Acteoside significantly inhibited arachidonic acid release and prostaglandin E2 production induced by 0.5 μM melittin. It is possible that Acteoside may be developed as an anti-inflammatory agent.
Ho Sun Song - One of the best experts on this subject based on the ideXlab platform.
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inhibitory effect of Acteoside on melittin induced catecholamine exocytosis through inhibition of ca2 dependent phospholipase a2 and extracellular ca2 influx in pc12 cells
Archives of Pharmacal Research, 2015Co-Authors: Ho Sun Song, Wan Kyunn Whang, Sang Soo SimAbstract:To investigate the inhibitory effect of Acteoside on the process of exocytosis induced by melittin, we measured Ca2+ mobilization, arachidonic acid (AA) release and catecholamine exocytosis in PC12 chromaffin cells. Melittin significantly increased the intracellular Ca2+ mobilization via receptor-operated calcium channel but not the intracellular Ca2+ release. It caused AA release via activation of Ca2+-dependent phospholipase A2 (PLA2) and catecholamine secretion in a dose-dependent manner. Acteoside dose-dependently inhibited the release of AA and intracellular Ca2+ mobilization induced by melittin. Acteoside reduced the catecholamine release and raised the amount of intracellular chromogranin A which is co-released with catecholamine from melittin-stimulated PC12 cells. Taken together, our results suggest that Acteoside could suppress the exocytosis via inhibition of Ca2+-dependent PLA2 and extracellular Ca2+ influx in PC12 cells stimulated by melittin.
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competitive inhibition of cytosolic ca2 dependent phospholipase a2 by Acteoside in rbl 2h3 cells
Archives of Pharmacal Research, 2012Co-Authors: Ho Sun Song, Wan Kyunn Whang, Mi Young Choi, Jae Min Jeong, Yong Ho Kim, Beom Hyeon Jang, Ji Hoon Sung, Min Gyu Kim, Sang Soo SimAbstract:The aim of this study was to investigate whether Acteoside isolated from Clerodendron trichotomum Thunberg may act as a selective inhibitor of phospholipase A(2) in RBL-2H3 cells. Acteoside dose-dependently inhibited 0.5 μM melittin-induced release of [(3)H]arachidonic acid, which was due to the inhibition of cytosolic Ca(2+)-dependent phospholipase A(2) (cPLA(2)) rather than secretory PLA(2) (sPLA(2)). In Dixon plots, the apparent K ( i ) value of Acteoside on cPLA(2) was 5.9 μM and the inhibitory pattern appeared to be a competitive inhibitor. The above data, suggests that Acteoside acts as a competitive inhibitor of cPLA(2) in RBL-2H3 cells.
Ming Yan - One of the best experts on this subject based on the ideXlab platform.
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Acteoside improved streptozotocin induced learning and memory impairment by upregulating hippocampal insulin glucose transport and energy metabolism
Phytotherapy Research, 2021Co-Authors: Jiayuan Chen, Li Gao, Yao Zhang, Zheng Kong, Dongqing Wang, Ming YanAbstract:Alzheimer's disease (AD), a neurodegenerative disease, has been, by and large, correlated to insulin pathway, glucose level, and energy metabolism in the brain. Intracerebroventricular administration of streptozotocin (ICV-STZ) leads to glucose and energy metabolism dysfunction, cognitive impairment, and increased oxidative stress in the brain. Acteoside has a myriad of pharmacological effects on the brain, namely, neuroprotection and recuperation of cognitive functions. The primary focus of the current study was to examine the effect of Acteoside on insulin, glucose transport, and energy metabolism in the hippocampal area of the brain. The behavioral experiments such as spatial memory, active learning, and passive memory suggested that acetoside ameliorated the ICV-STZ-induced learning and cognitive impairment. The Acteoside induced increase in the protein expression of glucose transporters (Glu T1, Glu T3, and Glu T4), glucose, and insulin levels in the hippocampus for maintaining normal learning and memory function were demonstrated by Western blot. In addition, Acteoside's long-term oral administration increased the the ratio of ATP content divided by ADP content (ATP/ADP) ratio, which, in turn, reduced the reactiveoxygen species (ROS) level and improved the cellular oxidative stress response. Compared with the model group, the above results show significant differences in different degrees (p < .05 or p < .01). This study suggests that Acteoside can ameliorate the ICV-STZ-induced learning and memory impairment caused due to insulin receptor, insulin receptor substrate 1, Glu T1, Glu T3, and Glu T4 pathways by triggering intracerebral metabolism.
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memory enhancement of Acteoside verbascoside in a senescent mice model induced by a combination of d gal and alcl3
Phytotherapy Research, 2015Co-Authors: Li Gao, Xiaoming Peng, Shixia Huo, Xinming Liu, Ming YanAbstract:Acteoside, also known as verbascoside or orobanchin, is a common compound found in many important medicinal plants including the Chinese herb Cistanche deserticola Y. C. Ma, which is used for its neuroprotective and memory enhancement properties. We have investigated the effects of Acteoside using a senescent mouse model induced by a combination of chronic intraperitoneal administration of d-gal (60 mg/kg/day) and oral administration AlCl3 (5 mg/kg/day) once daily for 90 days. After 60 days, Acteoside (30, 60, and 120 mg/kg/day) was orally administered once daily for 30 days. The memory enhancing effects of Acteoside were evaluated using the Morris water maze test. The results showed that 30–120 mg/kg/day of Acteoside reduced the escape latency in finding the platform, and increased the number of crossings of the platform. A 30–120 mg/kg/day of Acteoside increased significantly the expression of nerve growth factor and tropomycin receptor kinase A mRNA and protein in the hippocampus, measured using real-time RT-PCR, immunohistochemical analysis, and western blotting. These results support the use of C. deserticola for memory enhancement and indicate that the effects of Acteoside are induced via promotion of nerve growth factor and tropomycin receptor kinase A expression. Copyright © 2015 John Wiley & Sons, Ltd.
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pharmacokinetics of Acteoside following single dose intragastric and intravenous administrations in dogs
Chinese Journal of Natural Medicines, 2015Co-Authors: Wei Zhang, Yanli Wen, Han Xing, Di Zhao, Ming Yan, Shixia Huo, Qing Zhang, Xiaolin Sun, Xijing ChenAbstract:Acteoside (verbascoside), a phenylethanoid glycoside widely distributed in various plants, has been shown to have potential activity against Alzheimer's disease, attracting great attentions recently. The present study was designed to develop a selective and sensitive LC-MS/MS method for the determination of Acteoside in biological samples and carry our a pharmacokinetic (PK) study in beagle dogs. The PK parameters were calculated using non-compartmental models. Following a single-dose oral administration, Acteoside was rapidly absorbed and eliminated, with Tmax being between 30 to 45 min and terminal half-life being about 90 min. The areas under the time-concentration curve (AUC) were 47.28 ± 8.74, 87.86 ± 13.33, and 183.14 ± 28.69 mg · min · L(-1) for oral administration of 10, 20, and 40 mg · kg(-1), respectively, demonstrating that the exposure of Acteoside proportionally increased with the dose level. The absolute bioavailability of Acteoside was around 4%. For all the PK parameters, there were large variations between individual dogs. In conclusion, the pharmacokinetic characteristics observed in the present study can be of great value to help better understand the pharmacological properties of Acteoside and to improve the outcome of its clinical use.
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the mechanism of memory enhancement of Acteoside verbascoside in the senescent mouse model induced by a combination of d gal and alcl3
Phytotherapy Research, 2015Co-Authors: Xiaoming Peng, Li Gao, Shixia Huo, Xinmin Liu, Ming YanAbstract:Acteoside (verbsacoside), one of the main active phenylethanoid glycosides from Cistanche deserticola, is known to have antioxidant and neuroprotective activity, and herbs containing it are used to enhance memory. However, there is relatively little direct experimental evidence to support the use of Acteoside in Alzheimer's disease (AD). The purpose of this study was to elucidate the effects of Acteoside in improving learning and memory, using a mouse model of senescence induced by a combination of d-galactose and AlCl3, and investigate its potential mechanisms compared with the positive controls vitamin E and piracetam. Acteoside was administered intragastrically at doses of 30, 60 and 120 mg/kg/day for 30 days after AD was induced. Memory function was evaluated using a step-down test. The number of neuron was analysed by haematoxylin and eosin staining and the number of Nissl bodies by Nissl staining. The expression of caspase-3 protein in hippocampus was detected by immunohistochemistry and western blot. Nitric oxide and total nitric oxide synthase level in hippocampus were also assessed. Our results showed that the latency of step down was shortened in AD model mice and the number of errors decreased after treatment with all doses of Acteoside. Neurons and Nissl bodies in the hippocampus were increased significantly with higher doses (60 and 120 mg/kg/day) of Acteoside. The content of nitric oxide, the activity of nitric oxide synthase and the expression of caspase-3 protein were decreased by 120 mg/kg/day Acteoside compared with that of the AD model group. Our results support the results obtained previously using the Morris maze test in the same mouse model of senescence, and the use of traditional medicinal herbs containing Acteoside for neuroprotection and memory loss. Copyright © 2015 John Wiley & Sons, Ltd.
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protective effect of Acteoside on immunological liver injury induced by bacillus calmette guerin plus lipopolysaccharide
Planta Medica, 2009Co-Authors: Jun Zhao, Ming Yan, Tao Liu, Yu Zhao, Yi HuangAbstract:The hepatoprotective effects of Acteoside from O. coerulescens were evaluated in BCG plus LPS-induced immunological liver injury (ILI) in mice. Acteoside (50, 150, or 300 mg/kg) was administered via gavage daily for 12 days. The liver index (liver weight/body weight), liver homogenate levels of aspartate aminotransferase (AST) and alanine aminotransferase (ALT), hepatic nitric oxide (NO), malondialdehyde (MDA) content, superoxide dismutase (SOD) activity, production of tumor necrosis factor-gamma (TNF-gamma) and interleukin-2, 4, 10 (IL-2, 4, 10), as well as histopathological changes of the liver were evaluated following the 12-day treatment. Moreover, the modulation influence of Acteoside on the expression of B cell lymphoma/leukemia-2 (Bcl-2, hepatocyte apoptosis inhibitor) and Bcl-2 associated X protein (Bax, hepatocyte apoptosis promoter) in the mice liver with immunological hepatic injury was studied also. Acteoside (50, 150, or 300 mg/kg) effectively reduced the BCG/LPS-induced elevated liver index, liver homogenate AST and ALT levels, hepatic NO and MDA contents, restored hepatic SOD activity and reduced the degree of liver injury in ILI mice. The expression of Bax was decreased (vs. BCG + LPS model group), while the expression of Bcl-2 increased (vs. BCG + LPS model group). These results are close to those of DDB (as a reference drug), and suggest that Acteoside has a protective and therapeutic effect on ILI mice, which might be associated with its antioxidant properties, immunoregulatory function and regulation of hepatic apoptosis.
Jin-ao Duan - One of the best experts on this subject based on the ideXlab platform.
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a natural product of Acteoside ameliorate kidney injury in diabetes db db mice and hk 2 cells via regulating nadph oxidase tgf β smad signaling pathway
Phytotherapy Research, 2021Co-Authors: Qinwen Wang, Dawei Qian, Erxin Shang, Xinxin Dai, Xiang Xiang, Dandan Wei, Tianyao Zheng, Jin-ao DuanAbstract:This study was designed to investigate the protective effects and mechanisms of Acteoside on DKD in diabetes male db/db mice and high glucose-induced HK-2 cells. The diabetes db/db mice were divided randomly into model group, metformin group, irbesartan group, and Acteoside group. We observed the natural product of Acteoside exhibiting a significant effect in renal protection through analyzing of biochemical indicators and endogenous metabolites, histopathological observations, and western blotting. HK-2 cells subjected to high glucose were used in invitro experiments. The molecular mechanisms of them were investigated by RT-PCR and western blot. Acteoside prevents high glucose-induced HK-2 cells and diabetes db/db mice by inhibiting NADPH/oxidase-TGF-β/Smad signaling pathway. Acteoside regulated the disturbed metabolic pathway of lipid metabolism, glyoxylate and dicarboxylate metabolism, and arachidonic acid metabolism. We discovered the natural product of Acteoside exhibiting a significant effect in renal protection. This study paved the way for further exploration of pathogenesis, early diagnosis, and development of a new therapeutic agent for DKD.
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comparative pharmacokinetics of Acteoside from total glycoside extracted from leaves of rehmannia and dihuangye total glycoside capsule in normal and diabetic nephropathy rats
Biomedical Chromatography, 2017Co-Authors: Xinxin Dai, Dawei Qian, Erxin Shang, Dandan Wei, Tianyao Zheng, Hongdie Cai, Zhenhua Zhu, Hui Yan, Sheng Guo, Jin-ao DuanAbstract:Rehmannia glutinosa Libosch (RG), is officially listed in the Chinese Pharmacopoeia and is widely used in China. In this paper, a sensitive and rapid ultra-performance liquid chromatography-mass spectrometry method including multiple-reaction monitoring mode was developed and applied to study the pharmacokinetic effect of Acteoside from total glycoside extracted from the leaves of Rehmannia (TLR) and Dihuangye total glycoside capsule (DTG) in normal and diabetic nephropathy rats. The diabetic nephropathy rat model was induced by intraperitoneal injection of a small dose of streptozotocin and high-fat diet and plus 5% glucose drinking water. Samples of plasma of rats were obtained at different times after rats were administered TLR (7.2 g/kg) and DTG (360 mg/kg). After deproteinization by acetonitrile, the concentrations of Acteoside in rats at different time points were detected by UPLC-TQ-MS method and pharmacokinetics parameters were calculated using DAS 3.2.8 software. A good linearity of Acteoside was shown in the range of 8.51-3404.8 ng/m L (r2 = 0.9987). The mean extraction recovery of analyte was in the range of 63.55-79.49%, and the intra- and inter-day RSD values were <8.8%. Compared with the normal group, the maximum plasma concentration, AUC0-t , AUC0-∞ and apparent plasma clearance corresponding dose in model group rats decreased significantly. After rats were administered TLR and DTG, the Acteoside reached the maximum plasma concentration at about 15 min. The method proved to be simple, rapid and specific, and to be suitable for the determination of Acteoside in plasma of diabetic nephropathy rats and pharmacokinetic study.
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simultaneous determination of loganin morroniside catalpol and Acteoside in normal and chronic kidney disease rat plasma by uplc ms for investigating the pharmacokinetics of rehmannia glutinosa and cornus officinalis sieb drug pair extract
Journal of Chromatography B, 2016Co-Authors: Min Zhao, Dawei Qian, Pei Liu, Erxin Shang, Shu Jiang, Jianming Guo, Jinhua Tao, Jin-ao DuanAbstract:A sensitive and rapid method for determination of loganin, morroniside, catalpol and Acteoside in rat plasma after oral administration of Rehmannia glutinosa Libosch and Cornus officinalis Sieb drug pair based on ultra-high performance liquid chromatography coupled with tandem mass spectrometry (UPLC-MS). Chromatographic separation was achieved using an Acquity UPLC BEH C18 column (100mm×2.1mm, 1.7μm) at a flow rate of 0.4mL/min, using gradient mode containing 0.1% formic acid in water and acetonitrile were used as the mobile phase A and B. Loganin, morroniside, catalpol, Acteoside and the internal standard (chloramphenicol) were detected by selected reaction monitoring in the negative ion mode with the mass transition of m/z 451.0→179.0 (morroniside), m/z 435.0→227.0 (loganin), m/z 407.1→199.1 (catalpol), m/z 623.2→161.0 (Acteoside) and m/z 320.8→151.9 (chloramphenicol), respectively. All calibration curves showed good linearity (r>0.991). The precision was evaluated by intra-day and inter-day assays and the RSD% were all within 9.58%. The recovery ranged from 67.62 to 80.14%. The method was successfully applied to pharmacokinetic study of the analytes in normal and doxorubicin-induced chronic kidney disease rat plasma.
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comparative pharmacokinetics of catalpol and Acteoside in normal and chronic kidney disease rats after oral administration of rehmannia glutinosa extract
Biomedical Chromatography, 2015Co-Authors: Min Zhao, Dawei Qian, Erxin Shang, Shu Jiang, Jin-ao Duan, Shulan Su, Leyue DuAbstract:In this study, a sensitive and robust ultra-performance liquid chromatography–mass spectrometry method with multiple-reaction monitoring mode was developed, validated, and applied to determine pharmacokinetics of catalpol and Acteoside in normal and doxorubicin-induced chronic kidney disease rats after oral administration of Rehmannia glutinosa extract. The lower limits of quantification for catalpol and Acteoside in rat plasma were 2.62 and 0.61 ng/mL, with a signal-to-noise ratio of ≥10. Precision and accuracy studies showed that catalpol and Acteoside plasma concentrations were within the 10% range in all studies. The extraction recoveries of catalpol and Acteoside were both >68.24% and the matrix effects ranged from 96.59 to 101.62%. The method was successfully applied to the pharmacokinetic study of catalpol and Acteoside after oral administration of RG extract to normal and model rats, respectively. This study might further support the traditional use of RG to treat kidney diseases clinically. Copyright © 2015 John Wiley & Sons, Ltd.
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Comparative pharmacokinetics of catalpol and Acteoside in normal and chronic kidney disease rats after oral administration of Rehmannia glutinosa extract.
Biomedical chromatography : BMC, 2015Co-Authors: Min Zhao, Dawei Qian, Pei Liu, Erxin Shang, Shu Jiang, Jianming Guo, Jin-ao Duan, Jinhua TaoAbstract:In this study, a sensitive and robust ultra-performance liquid chromatography-mass spectrometry method with multiple-reaction monitoring mode was developed, validated, and applied to determine pharmacokinetics of catalpol and Acteoside in normal and doxorubicin-induced chronic kidney disease rats after oral administration of Rehmannia glutinosa extract. The lower limits of quantification for catalpol and Acteoside in rat plasma were 2.62 and 0.61 ng/mL, with a signal-to-noise ratio of ≥10. Precision and accuracy studies showed that catalpol and Acteoside plasma concentrations were within the 10% range in all studies. The extraction recoveries of catalpol and Acteoside were both >68.24% and the matrix effects ranged from 96.59 to 101.62%. The method was successfully applied to the pharmacokinetic study of catalpol and Acteoside after oral administration of RG extract to normal and model rats, respectively. This study might further support the traditional use of RG to treat kidney diseases clinically.
