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Liu Chang-jiang – One of the best experts on this subject based on the ideXlab platform.

  • Study on immune regulation of polysaccharide from Actinidia arguta
    Food and Fermentation Industries, 2013
    Co-Authors: Liu Chang-jiang

    Abstract:

    Actinidia arguta polysaccharide extracted by microwave was purified using a DEAE anion-exchange column,and SephadexG-100 column.The main elution fraction 0.1 brine elution was studied on the immunological activities of rat.The healthy rats were perfused with 2,10 and 20 mg/(kg·d) polysaccharides for 28 d.The effects of polysaccharide on the immune organs index,phagocytolysis of macrophage and spleen lymphocyte transformation index were determined.The results showed that,the low-dose group [2 mg/(kg·d)] of polysaccharide had almost no immunological activities.While the medium-dose group [10 mg/(kg·d)] had the obvious activities and was significant difference with the control group.Compared with the medium-dose group,the high-dose group [20 mg/(kg·d)] had no significant difference except immune organs index.

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  • Changes in Aroma Components of Actinidia arguta during Postharvest Storage at 20
    Food Science, 2012
    Co-Authors: Liu Chang-jiang

    Abstract:

    The released aroma components of Actinidia arguta(Sieb.et Zucc) fruits grown in Qianshan Mountain were extracted by solid-phase microextraction(SPME),separated and identified by gas chromatography-mass spectrometry(GC-MS).The changes in hardness and soluble solid content of Actinidia arguta were also analyzed during storage at 20 ℃.The results showed that the hardness of freshly picked fruits was 6.6 kg/cm2 and dropped to 0.95 kg/cm2 on the 10th day,soluble solid content rose from 8.50% to 11.45%.After 2,4,6,8 days and 10 days of storage,6,12,15,15 kinds and 15 kinds of aroma components were identified.A total of 22 compounds were identified from Actinidia arguta,and the major aroma components are terpinolene,β-pinene,p-cymene,D-limonene and α-pinene.

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  • Analysis of Essential Oil from Actinidia arguta by GC/MS with Pretreatment of Pectinase
    Food Research and Development, 2012
    Co-Authors: Liu Chang-jiang

    Abstract:

    The essential oils of ck processing and adding 1 % pectinase Actinidia arguta Sieb.et Zucc was obtained by using simultaneous distillation extraction(SDE),and chemical compounds were separated and identified by GC-MS.The results indicated that the compounds from two Actinidia arguta Sieb.et Zucc were different.20 chemical components were identified from ck processing.The major components in essential oil were aldehydes,alcohols,estes and other compounds.Accounting for 53.18 % of total volatile components.32 chemical components were identified from 1 % pectinase Actinidia arguta Sieb.et Zucc.The major chemical components were phenolic,alcohols,olefin and other compounds,accounting for 50.76 %,respectively.

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Sun Xiao-rong – One of the best experts on this subject based on the ideXlab platform.

  • Optimization of Ultrasound-assisted Extraction of Flavonoids from Actinidia arguta Fruits
    Food Science, 2011
    Co-Authors: Liu Chang-jiang, Wang Fei, Luan Yunfeng, Sun Xiao-rong

    Abstract:

    Objective: To explore the optimal extraction conditions of flavonoids from the fruits of Actinidia arguta.Methods: Flavonoids extraction was carried out using ethanol as the extraction solvent with the assistance of ultrasound.Response surface methodology based on Box-Behnken experimental design was used for process optimization.Results: The optimal extraction conditions were ethanol concentration of 70%,ultrasonic treatment time of 6 min and ultrasonic power of 270 W.Under these conditions,the experimental extraction rate of total flavonoids from Actinidia arguta fruits was 0.0297%,which was consistent with the prediction value.Conclusion: Ultrasound assistance is a suitable method for the extraction of total flavonoids from Actinidia arguta fruits.

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  • Optimization of Extraction of Actinidia arguta Sieb.et Zucc Protein Using Response Surface Methodology
    Food Research and Development, 2011
    Co-Authors: Sun Xiao-rong

    Abstract:

    The effects of extracting time,pH value and the ratio of fluid-solid,on the yield of protein from Actinidia arguta Sieb.et Zucc were studied and the parameters for optimum yield were obtained by using RAS software.The results showed that the optimum parameters were: extracting time was 2.3 hours,pH was 7.9,and the ratio of fluid-solid was 19∶1 mL/g.The protein extraction rate was 53.23 % under these conditions.

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  • Optimization of Microwave-assisted Extraction Conditions of Actinidia arguta Flavonoids Using Response Surface Methodology
    Food Research and Development, 2010
    Co-Authors: Sun Xiao-rong

    Abstract:

    In this study,optimum condition of microwave-assisted extraction of Actinidia arguta is optimized by response surface test.On the basis of single-factor test,the mathematical regression model is established on the dependent variable(extraction yield of Actinidia arguta flavonoids) and independent variables(ethanol concentration,microwave power and extraction time) by Box-Benhnken center composite design and response surface methodology.The optimum extraction conditions are as follows:ethanol concentration 76 %,extraction time 6 min,microwave power 300 W.Under these conditions,the yield of Actinidia arguta flavonoids is 0.322 mg/g,consistent with the prediction value.

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Chang-jiang Liu – One of the best experts on this subject based on the ideXlab platform.

  • Actinidia arguta Polysaccharide Induces Apoptosis in Hep G2 Cells
    Advance Journal of Food Science and Technology, 2015
    Co-Authors: Chang-jiang Liu, Ying Liu, Chang-hua Tan, Yang-yang Liu

    Abstract:

    In our previous study, a potential Antitumor Polysaccharide (AAP-3b) was extracted from the fruit of Actinidia arguta. Several earlier studies have indicated that AAP-3b exhibits antioxidant activity. This study was designed to determine the effect of AAP-3b on cytotoxicity in human hepatoma cell line, Hep G2 cells. Hep G2 cells were cultured in the presence of AAP-3b at various concentrations (0.005-1 mg/mL) from 24 to 96 h and the percentage of cell viability was evaluated by 3-(4, 5-dimethylthiazol-2-yl)-2, 5-diphenyl Tetrazolium bromide (MTT) assay. The results showed that AAP-3b inhibited the cells viability in concentration and time dependent characteristics at concentrations (0.05-0.5 mg/mL) from 24 to 72 h. The IC50 (50% cell growth inhibitory concentration) of tumor cell proliferation inhibition from 24 to 72 h were 0.193 (95% confidence interval 0.159 to 0.234), 0.114 (95% confidence interval 0.073 to 0.170), 0.076 (95% confidence interval 0.034 to 0.137) mg/mL respectively. We found that anti-proliferative effect of AAP-3b was associated with cell cycle blocked and apoptosis on Hep G2 cells by determinations of morphological changes, cell cycle and apoptosis. These results provide new insight into the health functions of Actinidia arguta polysaccharide.

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  • Extraction Process Optimization of Total Alkaloid from Actinidia arguta
    Proceedings of the 2015 International Conference on Materials Environmental and Biological Engineering, 2015
    Co-Authors: Yang-yang Liu, Chang-jiang Liu

    Abstract:

    To study on optimal conditions for extracting the higher content of total alkaloid from Actinidia arguta fruits, the effects of ethanol concentration, temperature, time and solid-liquid ratio on extraction efficiency total alkaloid were explored. The results showed that the optimum extraction conditions included 70% ethanol as extracting solution, temperature on 60C, extraction time by 20 min, ratio of solid- liquid on 1:30 (g/mL). By this method, the total alkaloids of Actinidia arguta fruits were extracted to 1.021 mg/g. This experiment of extraction method is simple and easy to operate. It was improved the extraction yield of the total alkaloid extraction from Actinidia arguta fruits.

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  • Determination of Total Alkaloids in Different Parts of Actinidia arguta by Spectrophotography
    Proceedings of the 2015 International Conference on Materials Environmental and Biological Engineering, 2015
    Co-Authors: Yang-yang Liu, Chang-jiang Liu

    Abstract:

    Total Alkaloids are important active materials of Actinidia arguta. However, no study has been conducted to explore the content determination method of the Actinidia arguta total alkaloid. An effective method was established in order to determine the total alkaloids from different parts of Actinidia arguta. Using ultrasonic cleaner, the total alkaloids were extracted from the samples and tested at 415nm with the spectroscopy by the means of acid dye method. The calibration curve of aconitine were linear (r=0.9997), and the recovery rate was prefect (98.9%). The results of measurement were consistent in 3 hours. The content of alkaloids in roots was the highest, with 1.25mg/g, followed by leaves and fruits, the least were stems. The method is simple and effective, and the contents of total alkaloids were different in different parts of Actinidia arguta.

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