The Experts below are selected from a list of 1179 Experts worldwide ranked by ideXlab platform

Liu Chang-jiang - One of the best experts on this subject based on the ideXlab platform.

  • Study on immune regulation of polysaccharide from Actinidia arguta
    Food and Fermentation Industries, 2013
    Co-Authors: Liu Chang-jiang
    Abstract:

    Actinidia arguta polysaccharide extracted by microwave was purified using a DEAE anion-exchange column,and SephadexG-100 column.The main elution fraction 0.1 brine elution was studied on the immunological activities of rat.The healthy rats were perfused with 2,10 and 20 mg/(kg·d) polysaccharides for 28 d.The effects of polysaccharide on the immune organs index,phagocytolysis of macrophage and spleen lymphocyte transformation index were determined.The results showed that,the low-dose group [2 mg/(kg·d)] of polysaccharide had almost no immunological activities.While the medium-dose group [10 mg/(kg·d)] had the obvious activities and was significant difference with the control group.Compared with the medium-dose group,the high-dose group [20 mg/(kg·d)] had no significant difference except immune organs index.

  • Changes in Aroma Components of Actinidia arguta during Postharvest Storage at 20
    Food Science, 2012
    Co-Authors: Liu Chang-jiang
    Abstract:

    The released aroma components of Actinidia arguta(Sieb.et Zucc) fruits grown in Qianshan Mountain were extracted by solid-phase microextraction(SPME),separated and identified by gas chromatography-mass spectrometry(GC-MS).The changes in hardness and soluble solid content of Actinidia arguta were also analyzed during storage at 20 ℃.The results showed that the hardness of freshly picked fruits was 6.6 kg/cm2 and dropped to 0.95 kg/cm2 on the 10th day,soluble solid content rose from 8.50% to 11.45%.After 2,4,6,8 days and 10 days of storage,6,12,15,15 kinds and 15 kinds of aroma components were identified.A total of 22 compounds were identified from Actinidia arguta,and the major aroma components are terpinolene,β-pinene,p-cymene,D-limonene and α-pinene.

  • Analysis of Essential Oil from Actinidia arguta by GC/MS with Pretreatment of Pectinase
    Food Research and Development, 2012
    Co-Authors: Liu Chang-jiang
    Abstract:

    The essential oils of ck processing and adding 1 % pectinase Actinidia arguta Sieb.et Zucc was obtained by using simultaneous distillation extraction(SDE),and chemical compounds were separated and identified by GC-MS.The results indicated that the compounds from two Actinidia arguta Sieb.et Zucc were different.20 chemical components were identified from ck processing.The major components in essential oil were aldehydes,alcohols,estes and other compounds.Accounting for 53.18 % of total volatile components.32 chemical components were identified from 1 % pectinase Actinidia arguta Sieb.et Zucc.The major chemical components were phenolic,alcohols,olefin and other compounds,accounting for 50.76 %,respectively.

  • Optimization of Cellulase Pretreatment for Microwave-Assisted Extraction of Flavonoids from Actinidia arguta Stem
    Food Science, 2012
    Co-Authors: Liu Chang-jiang
    Abstract:

    Cellulase pretreatment before microwave-assisted extraction of flavonoids from Actinidia arguta stem was optimized by response surface methodology.One-factor-at-a-time experiments were performed to select appropriate levels of enzyme dose,hydrolysis time,temperature and pH.Further,a mathematical model was built and subjected to variance analysis.The optimal cellulase hydrolysis conditions were determined as follows: the optimal enzyme dose was 438 U/g for 64 min of hydrolysis at 49 ℃ and pH 4.7.Under these conditions,the extraction rate of flavonoids was up to 2.89%.

  • Antioxidant Activity in vitro of Total Flavonoids from Actinidia arguta
    Food Science, 2011
    Co-Authors: Liu Chang-jiang
    Abstract:

    Objective: To investigate the antioxidant activity in vitro of total flavonoids from Actinidia arguta.Methods: The scavenging capacities of total flavonoids from Actinidia arguta against DPPH,superoxide anion and hydroxyl free radicals the ferric reducing power and the inhibitory effect on lipid peroxidation were determined spectrometrically and compared with those of vitamin C.Results: Total flavonoids from Actinidia arguta revealed a strong ability to scavenge DPPH free radicals,reduce ferric ions and inhibit lipid peroxidation and had a certain scavenging effect against superoxide anion and hydroxyl free radicals in a dose-dependent manner.Conclusion: Total flavonoids from Actinidia arguta have excellent antioxidant activity.

Sun Xiao-rong - One of the best experts on this subject based on the ideXlab platform.

  • Optimization of Extraction of Actinidia arguta Sieb.et Zucc Protein Using Response Surface Methodology
    Food Research and Development, 2011
    Co-Authors: Sun Xiao-rong
    Abstract:

    The effects of extracting time,pH value and the ratio of fluid-solid,on the yield of protein from Actinidia arguta Sieb.et Zucc were studied and the parameters for optimum yield were obtained by using RAS software.The results showed that the optimum parameters were: extracting time was 2.3 hours,pH was 7.9,and the ratio of fluid-solid was 19∶1 mL/g.The protein extraction rate was 53.23 % under these conditions.

  • Optimization of Ultrasound-assisted Extraction of Flavonoids from Actinidia arguta Fruits
    Food Science, 2011
    Co-Authors: Liu Chang-jiang, Wang Fei, Luan Yunfeng, Sun Xiao-rong
    Abstract:

    Objective: To explore the optimal extraction conditions of flavonoids from the fruits of Actinidia arguta.Methods: Flavonoids extraction was carried out using ethanol as the extraction solvent with the assistance of ultrasound.Response surface methodology based on Box-Behnken experimental design was used for process optimization.Results: The optimal extraction conditions were ethanol concentration of 70%,ultrasonic treatment time of 6 min and ultrasonic power of 270 W.Under these conditions,the experimental extraction rate of total flavonoids from Actinidia arguta fruits was 0.0297%,which was consistent with the prediction value.Conclusion: Ultrasound assistance is a suitable method for the extraction of total flavonoids from Actinidia arguta fruits.

  • Optimization of Microwave-assisted Extraction Conditions of Actinidia arguta Flavonoids Using Response Surface Methodology
    Food Research and Development, 2010
    Co-Authors: Sun Xiao-rong
    Abstract:

    In this study,optimum condition of microwave-assisted extraction of Actinidia arguta is optimized by response surface test.On the basis of single-factor test,the mathematical regression model is established on the dependent variable(extraction yield of Actinidia arguta flavonoids) and independent variables(ethanol concentration,microwave power and extraction time) by Box-Benhnken center composite design and response surface methodology.The optimum extraction conditions are as follows:ethanol concentration 76 %,extraction time 6 min,microwave power 300 W.Under these conditions,the yield of Actinidia arguta flavonoids is 0.322 mg/g,consistent with the prediction value.

Chang-jiang Liu - One of the best experts on this subject based on the ideXlab platform.

  • Actinidia arguta Polysaccharide Induces Apoptosis in Hep G2 Cells
    Advance Journal of Food Science and Technology, 2015
    Co-Authors: Chang-jiang Liu, Ying Liu, Chang-hua Tan, Yang-yang Liu
    Abstract:

    In our previous study, a potential Antitumor Polysaccharide (AAP-3b) was extracted from the fruit of Actinidia arguta. Several earlier studies have indicated that AAP-3b exhibits antioxidant activity. This study was designed to determine the effect of AAP-3b on cytotoxicity in human hepatoma cell line, Hep G2 cells. Hep G2 cells were cultured in the presence of AAP-3b at various concentrations (0.005-1 mg/mL) from 24 to 96 h and the percentage of cell viability was evaluated by 3-(4, 5-dimethylthiazol-2-yl)-2, 5-diphenyl Tetrazolium bromide (MTT) assay. The results showed that AAP-3b inhibited the cells viability in concentration and time dependent characteristics at concentrations (0.05-0.5 mg/mL) from 24 to 72 h. The IC50 (50% cell growth inhibitory concentration) of tumor cell proliferation inhibition from 24 to 72 h were 0.193 (95% confidence interval 0.159 to 0.234), 0.114 (95% confidence interval 0.073 to 0.170), 0.076 (95% confidence interval 0.034 to 0.137) mg/mL respectively. We found that anti-proliferative effect of AAP-3b was associated with cell cycle blocked and apoptosis on Hep G2 cells by determinations of morphological changes, cell cycle and apoptosis. These results provide new insight into the health functions of Actinidia arguta polysaccharide.

  • Extraction Process Optimization of Total Alkaloid from Actinidia arguta
    Proceedings of the 2015 International Conference on Materials Environmental and Biological Engineering, 2015
    Co-Authors: Yang-yang Liu, Chang-jiang Liu
    Abstract:

    To study on optimal conditions for extracting the higher content of total alkaloid from Actinidia arguta fruits, the effects of ethanol concentration, temperature, time and solid-liquid ratio on extraction efficiency total alkaloid were explored. The results showed that the optimum extraction conditions included 70% ethanol as extracting solution, temperature on 60C, extraction time by 20 min, ratio of solid- liquid on 1:30 (g/mL). By this method, the total alkaloids of Actinidia arguta fruits were extracted to 1.021 mg/g. This experiment of extraction method is simple and easy to operate. It was improved the extraction yield of the total alkaloid extraction from Actinidia arguta fruits.

  • Determination of Total Alkaloids in Different Parts of Actinidia arguta by Spectrophotography
    Proceedings of the 2015 International Conference on Materials Environmental and Biological Engineering, 2015
    Co-Authors: Yang-yang Liu, Chang-jiang Liu
    Abstract:

    Total Alkaloids are important active materials of Actinidia arguta. However, no study has been conducted to explore the content determination method of the Actinidia arguta total alkaloid. An effective method was established in order to determine the total alkaloids from different parts of Actinidia arguta. Using ultrasonic cleaner, the total alkaloids were extracted from the samples and tested at 415nm with the spectroscopy by the means of acid dye method. The calibration curve of aconitine were linear (r=0.9997), and the recovery rate was prefect (98.9%). The results of measurement were consistent in 3 hours. The content of alkaloids in roots was the highest, with 1.25mg/g, followed by leaves and fruits, the least were stems. The method is simple and effective, and the contents of total alkaloids were different in different parts of Actinidia arguta.

  • Study on Specific Enzymes in Actinidia arguta Fruit Softening
    Lecture Notes in Electrical Engineering, 2013
    Co-Authors: Li Shuqian, Chang-jiang Liu, Guang Xin, Bo Zhang
    Abstract:

    Actinidia arguta fruit postharvest softening has two phases: the first stage, softening faster plays a main role with the phases of amylase enzymes seven: the Second stage softening slowly, has the main effect since the phases of enzymes are together with more than half of lactose hyaluronic acid enzymes. Ethylene release promotes the role of fruit softening protective enzyme peroxidase, catalane appears in late fruit softening, so no fruit softening in the phases of enzyme specificity.

Shen Yu-jie - One of the best experts on this subject based on the ideXlab platform.

  • Study on the Diurnal Variation of Photosynthesis of Actinidia arguta(Seib.et Zucc.)Plangch.ex Miq.
    Northern Horticulture, 2010
    Co-Authors: Shen Yu-jie
    Abstract:

    In order to understand the eco-physiological characteristics of Actinidia arguta(Seib.et Zucc.)Plangch.ex Miq.,the studies on diurnal chang of photosynthetic characteristics of Actinidia arguta(Seib.et Zucc.)Plangch.ex Miq.And its environmental factors were conducted by the CIRAS-2 portable photosynthetic system.The results indicated that daily variation of net photosynthesis rate of Actinidia arguta(Seib.et Zucc.)Plangch.ex Miq.showed a bimodal type and obvious characteristics of photosynthesis mid-nap.The net photosynthesis rate of Actinidia arguta(Seib.et Zucc.)Plangch.ex Miq.was closely related to photosynthesis active radiation,stomatal conductance and intercellular CO2 concentrations.

  • Responses of Net Photosynthetic Rate to Light Intensity and CO_2 Concentration in Actinidia arguta(Seib.et Zucc.) Plangch.ex Miq.
    Northern Horticulture, 2010
    Co-Authors: Shen Yu-jie
    Abstract:

    This experiment was to study the responses of net photosynthetic rate to light intensity and CO2 concentration in different series Actinidia arguta(Seib.et Zucc.) Plangch.ex Miq.by the CIRAS-2 portable photosynthetic system.The results showed that the net photosynthetic rate of three different Actinidia arguta(Seib.et Zucc.) Plangch.ex Miq.increased with the raising of light intensity and the concentration of CO2.The light saturation point for the cultivar kuilv and two strains 8401,9701 were 1 590 μmol·m-2·s-1,1 321 μmol·m-2·s-1 and 1 670 μmol·m-2·s-1 respectively,and the light compensation point were 51 μmol·m-2·s-1,9 μmol·m-2·s-1,33 μmol·m-2·s-1.The CO2 saturation and compensation point were 1 290 μmol·mol-1,1 316 μmol·mol-1,1 280 μmol·mol-1,119 μmol·mol-1,123 μmol·mol-1 and 63 μmol·mol-1.In conclusion,the Actinidia arguta(Seib.et Zucc.) Plangch.ex Miq.had the same characters with heliophyte,while 8401 was resistive to low light and could be plant in the woods,the character showed that 9701 was a excellent strains with well-adapted and higher production.

Hai-feng Zhang - One of the best experts on this subject based on the ideXlab platform.

  • Flavonoid and saponin rich fractions of kiwi roots (Actinidia arguta (Sieb.et Zucc.) Planch) with antinociceptive and anti-inflammatory effects
    African Journal of Pharmacy and Pharmacology, 2013
    Co-Authors: Kun Teng, Hong-sheng Ruan, Hai-feng Zhang
    Abstract:

    The roots of kiwi (Actinidia arguta (Sieb.et Zucc.) Planch) have medicinal uses as anti-tumour, antinociceptive and anti-inflammatory agents. We aimed to evaluate the antinociceptive and anti-inflammatory effects of the major fractions of kiwi roots. The ethanolic extract of the plant roots was partitioned using a liquid–liquid extraction procedure to give five major fractions. Following phytochemical screening of isolated fractions, the total extract and each fraction were evaluated for their antinociception and anti-inflammatory effects using acetic acid, hot plate test, formalin and carrageenan-induced paw edema tests, respectively. The results indicated that the total extract, ethyl acetate fraction (EAF) and n-butanol fraction (BF) exhibited significant inhibition of acetic acid-induced writhing, and both phases of the formalin-induced pain response increased in time of response to thermal stimulation in hot plate test and exhibited significant dose-related inhibition of carrageenan induced paw oedema volumes when compared with the control group. It can be concluded that the flavonoid and saponin content of kiwi roots can be responsible for antinociception and anti-inflammatory effects of the plant, respectively.   Key words: Actinidia arguta, anti-inflammatory, antinociceptive, flavonoid, saponin.