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Xiangdong Wang - One of the best experts on this subject based on the ideXlab platform.
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regulatory roles of hspa6 in Actinidia chinensis planch root extract acroots inhibited lung cancer proliferation
Clinical and translational medicine, 2020Co-Authors: Lingyan Wang, Jiayun Hou, Jianxin Wang, Zhenghua Zhu, Wei Zhang, Xuemei Zhang, Hui Shen, Xiangdong WangAbstract:Actinidia chinensis Planch. root extract (acRoots) as one of Chinese traditional medications has been applied for antitumor therapy for decades, although the exact mechanisms have not been revealed. Our present study aimed to define the inhibitory specificity and pattern of acRoots in the lung cancer cell lines by comparing 40 types of cancer cell lines, select acRoots-associated inflammation target genes from transcriptional profiles of acRoots-sensitive and less-sensitive lung cancer cell lines, and validate the correlation of acRoots-associated inflammation target genes with prognosis of patients with lung cancer. We selected acRoots-sensitive (H1299) and less-sensitive lung cancer cells (H460) and found that the sensitivity was associated with the appearance of p53. The heat shock 70 kDa protein 6 (HSPA6) was defined as a critical factor in regulating cell sensitivity probably through the interaction with intra-HSPA family members, inter-HSP family members, and other families. The degree of cell sensitivity to acRoots increased in both sensitive and less-sensitive cells after deletion of HSPA6 genes. Thus, our data indicate that HSPA6 and HSPA6-dominated molecular network can be an alternative to modify cell sensitivity to drugs.
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regulatory roles of oasl in lung cancer cell sensitivity to Actinidia chinensis planch root extract acroots
Cell Biology and Toxicology, 2018Co-Authors: Lingyan Wang, Xiangdong Wang, Hui ShenAbstract:Lung cancer is one of the most common malignancies worldwide. Actinidia chinensis Planch root extract (acRoots) was found to have the capacity of the anti-tumor, although the molecular mechanisms remain unclear. The present study aims to investigate the molecular mechanisms by which lung cancer cells sense to inhibitory effects of acRoots with a special focus on immune-associated gene profiles. We firstly provide a preclinical evidence that acRoots can significantly inhibit lung cancer cell proliferation and apoptosis via the PI3K-OASL signal pathway. The heterogeneous alterations of immune-associated gene profiles of lung cancer cell types were measured after treatment with various doses of acRoots. The OASL gene was identified as the key regulator in molecular networks of acRoots-treated lung cancer cells and validated. The OASL gene plays an important role in the regulation of lung cancer cell sensitivity to acRoots, which modulated by the PI3K signal pathway. Thus, our data indicate that OASL can be one of the decisive regulators to maintain lung cancer cell susceptibility to acRoots and may be associated with the development of drug resistance. The regulation of OASL can be an alternative strategy to improve drug efficacy during cancer therapies.
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Actinidia chinensis planch root extract inhibits cholesterol metabolism in hepatocellular carcinoma through upregulation of pcsk9
Oncotarget, 2017Co-Authors: Jiayun Hou, Lingyan Wang, Minghuan Zheng, Tingting Fang, Xiangdong Wang, Jinglin XiaAbstract:// Mingyan He 1, * , Jiayun Hou 2, * , Lingyan Wang 2, * , Minghuan Zheng 2 , Tingting Fang 1 , Xiangdong Wang 2 and Jinglin Xia 1, 3 1 Liver Cancer Institute, Zhongshan Hospital, Fudan University, Shanghai, China 2 Clinical Science Institute, Zhongshan Hospital, Fudan University, Shanghai, China 3 Minhang Hospital, Fudan University, Shanghai, China * These authors have contributed equally to this work Correspondence to: Jinglin Xia, email: xiajinglin@fudan.edu.cn Xiangdong Wang, email: xianagdong.wang@clintransmed.org Keywords: hepatocellular carcinoma, root of Actinidia chinensis, PCSK9, cholesterol metabolism Received: September 09, 2016 Accepted: January 16, 2017 Published: February 02, 2017 ABSTRACT Actinidia chinensis Planch root extract (acRoots) is a traditional Chinese medicine with anti-tumor efficacy. To investigate the mechanisms responsible for this activity, we examined the effects of acRoots on cholesterol metabolism in hepatocellular carcinoma (HCC). mRNA chip analysis was used to identify the metabolic genes regulated by acRoots. The effects of acRoots on cholesterol synthesis and uptake were evaluated by measuring intracellular cholesterol levels and 3,3’-dioctadecylindocarbocyanine-labeled low-density lipoprotein (Dil-LDL) uptake. Expression of metabolic genes was analyzed using quantitative reverse transcription PCR, western blotting, and flow cytometry. acRoots reduced the viability of LM3 and HepG2 cells at 5 mg/mL and HL-7702 cells at 30 mg/mL. Gene expression profiling revealed that treatment with acRoots altered expression of genes involved in immune responses, inflammation, proliferation, cell cycle control, and metabolism. We also confirmed that acRoots enhances expression of PCSK9 , which is important for cholesterol metabolism. This resulted in decreased LDL receptor expression, inhibition of LDL uptake by LM3 cells, decreased total intracellular cholesterol, and reduced proliferation. These effects were promoted by PCSK9 overexpression and rescued by PCSK9 knockdown. Our data demonstrate that acRoots is a novel anti-tumor agent that inhibits cholesterol metabolism though a PCSK9 -mediated signaling pathway.
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Actinidia chinensis planch root extract acroots inhibits hepatocellular carcinoma progression by inhibiting ep3 expression
Cell Biology and Toxicology, 2016Co-Authors: Tingting Fang, Jiayun Hou, Lingyan Wang, Minghuan Zheng, Xiangdong Wang, Jinglin XiaAbstract:A wide range of studies has demonstrated the potent anticancer activity of Chinese herbs. Here, we evaluated the anticancer activity and molecular mechanisms of Actinidia chinensis root extract (acRoots) on hepatocellular carcinoma (HCC). HepG2 HCC cells were treated with various concentrations of acRoots for 72 h and examined by mRNA expression profiling, revealing alterations in cellular immunity, inflammation, proliferation, cell cycle, and metabolic signaling responses. Further analysis of the altered genes in cellular immunity and inflammation gene clusters identified prostaglandin E receptor 3 (EP3) as a key regulator of gene expression in response to acRoots. Further analysis revealed inhibition of cell growth, migration, and invasion in HCC in response to acRoots, along with increased apoptosis due to downregulation of EP3 expression. Treatment with acRoots and EP3 antagonist L-798106 led to decreases in VEGF, EGFR, MMP2, and MMP9 expression in HCC cells, along with significant effects on growth, migration, invasion, and apoptosis; the effects were reversed/blocked by the EP3 agonist sulprostone. Taken together, these data clearly demonstrated that acRoots inhibit HCC cell invasion and metastasis via inhibition of EP3 expression, resulting in decreased activation of VEGF, EGFR, MMP2, and MMP9.
Jinglin Xia - One of the best experts on this subject based on the ideXlab platform.
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Actinidia chinensis planch root extract attenuates proliferation and metastasis of hepatocellular carcinoma by inhibiting epithelial mesenchymal transition
Journal of Ethnopharmacology, 2019Co-Authors: Tingting Fang, Yuan Fang, Zhiying Zhao, Peixin Huang, Feifei Yuan, Mengzhou Guo, Biwei Yang, Jinglin XiaAbstract:Abstract Ethno-pharmacological relevance Numerous studies have demonstrated the potent anticancer activity of various Chinese herbs. Actinidia chinensis Planch root (acRoots), a traditional Chinese medicine, functions as an antitumor and detoxifying agent and plays a role in diuresis and hemostasis. Treatment with acRoots confers strong inhibition of tumor growth in various forms of cancer. Here, we evaluated the anticancer activity and molecular mechanisms of Actinidia chinensis Planch root extract (acRoots) on hepatocellular carcinoma (HCC). Materials and methods Our previous study used mRNA chip analyses to identify the genes regulated by acRoots. Further analyses of the altered genes identified a key regulator of genes in response to acRoots. Here, the effects of acRoots on HCC cell proliferation, migration, invasion, and apoptosis were evaluated by cell counting, Transwell and apoptosis assays. In addition, the in vivo anti-HCC effects of acRoots were investigated using an HCC animal model. The expression of a key regulator of genes in response to acRoots was analyzed using quantitative polymerase chain reaction and western blotting. Results Treatment with acRoots (10 mg/mL) had no cytotoxicity in L02 cells and had a positive effect on L02 cell viability; however, it significantly inhibited HCC cell proliferation. Treatment with acRoots downregulated DLX2 gene expression in HCC cells, and high DLX2 expression was associated with advanced stage and poor prognosis in patients with HCC. Treatment with acRoots inhibited proliferation, invasion and migration, clonality, and the epithelial-to-mesenchymal transition, and promoted the apoptosis of HCC cells by downregulating DLX2 expression. HCC cells with higher DLX2 expression were more sensitive to acRoots. Conclusions acRoots inhibited the malignant biological behavior of HCC cells via regulation of the epithelial-mesenchymal transition (EMT) by DLX2.
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Actinidia chinensis planch root extract inhibits cholesterol metabolism in hepatocellular carcinoma through upregulation of pcsk9
Oncotarget, 2017Co-Authors: Jiayun Hou, Lingyan Wang, Minghuan Zheng, Tingting Fang, Xiangdong Wang, Jinglin XiaAbstract:// Mingyan He 1, * , Jiayun Hou 2, * , Lingyan Wang 2, * , Minghuan Zheng 2 , Tingting Fang 1 , Xiangdong Wang 2 and Jinglin Xia 1, 3 1 Liver Cancer Institute, Zhongshan Hospital, Fudan University, Shanghai, China 2 Clinical Science Institute, Zhongshan Hospital, Fudan University, Shanghai, China 3 Minhang Hospital, Fudan University, Shanghai, China * These authors have contributed equally to this work Correspondence to: Jinglin Xia, email: xiajinglin@fudan.edu.cn Xiangdong Wang, email: xianagdong.wang@clintransmed.org Keywords: hepatocellular carcinoma, root of Actinidia chinensis, PCSK9, cholesterol metabolism Received: September 09, 2016 Accepted: January 16, 2017 Published: February 02, 2017 ABSTRACT Actinidia chinensis Planch root extract (acRoots) is a traditional Chinese medicine with anti-tumor efficacy. To investigate the mechanisms responsible for this activity, we examined the effects of acRoots on cholesterol metabolism in hepatocellular carcinoma (HCC). mRNA chip analysis was used to identify the metabolic genes regulated by acRoots. The effects of acRoots on cholesterol synthesis and uptake were evaluated by measuring intracellular cholesterol levels and 3,3’-dioctadecylindocarbocyanine-labeled low-density lipoprotein (Dil-LDL) uptake. Expression of metabolic genes was analyzed using quantitative reverse transcription PCR, western blotting, and flow cytometry. acRoots reduced the viability of LM3 and HepG2 cells at 5 mg/mL and HL-7702 cells at 30 mg/mL. Gene expression profiling revealed that treatment with acRoots altered expression of genes involved in immune responses, inflammation, proliferation, cell cycle control, and metabolism. We also confirmed that acRoots enhances expression of PCSK9 , which is important for cholesterol metabolism. This resulted in decreased LDL receptor expression, inhibition of LDL uptake by LM3 cells, decreased total intracellular cholesterol, and reduced proliferation. These effects were promoted by PCSK9 overexpression and rescued by PCSK9 knockdown. Our data demonstrate that acRoots is a novel anti-tumor agent that inhibits cholesterol metabolism though a PCSK9 -mediated signaling pathway.
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Actinidia chinensis planch root extract acroots inhibits hepatocellular carcinoma progression by inhibiting ep3 expression
Cell Biology and Toxicology, 2016Co-Authors: Tingting Fang, Jiayun Hou, Lingyan Wang, Minghuan Zheng, Xiangdong Wang, Jinglin XiaAbstract:A wide range of studies has demonstrated the potent anticancer activity of Chinese herbs. Here, we evaluated the anticancer activity and molecular mechanisms of Actinidia chinensis root extract (acRoots) on hepatocellular carcinoma (HCC). HepG2 HCC cells were treated with various concentrations of acRoots for 72 h and examined by mRNA expression profiling, revealing alterations in cellular immunity, inflammation, proliferation, cell cycle, and metabolic signaling responses. Further analysis of the altered genes in cellular immunity and inflammation gene clusters identified prostaglandin E receptor 3 (EP3) as a key regulator of gene expression in response to acRoots. Further analysis revealed inhibition of cell growth, migration, and invasion in HCC in response to acRoots, along with increased apoptosis due to downregulation of EP3 expression. Treatment with acRoots and EP3 antagonist L-798106 led to decreases in VEGF, EGFR, MMP2, and MMP9 expression in HCC cells, along with significant effects on growth, migration, invasion, and apoptosis; the effects were reversed/blocked by the EP3 agonist sulprostone. Taken together, these data clearly demonstrated that acRoots inhibit HCC cell invasion and metastasis via inhibition of EP3 expression, resulting in decreased activation of VEGF, EGFR, MMP2, and MMP9.
Lingyan Wang - One of the best experts on this subject based on the ideXlab platform.
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regulatory roles of hspa6 in Actinidia chinensis planch root extract acroots inhibited lung cancer proliferation
Clinical and translational medicine, 2020Co-Authors: Lingyan Wang, Jiayun Hou, Jianxin Wang, Zhenghua Zhu, Wei Zhang, Xuemei Zhang, Hui Shen, Xiangdong WangAbstract:Actinidia chinensis Planch. root extract (acRoots) as one of Chinese traditional medications has been applied for antitumor therapy for decades, although the exact mechanisms have not been revealed. Our present study aimed to define the inhibitory specificity and pattern of acRoots in the lung cancer cell lines by comparing 40 types of cancer cell lines, select acRoots-associated inflammation target genes from transcriptional profiles of acRoots-sensitive and less-sensitive lung cancer cell lines, and validate the correlation of acRoots-associated inflammation target genes with prognosis of patients with lung cancer. We selected acRoots-sensitive (H1299) and less-sensitive lung cancer cells (H460) and found that the sensitivity was associated with the appearance of p53. The heat shock 70 kDa protein 6 (HSPA6) was defined as a critical factor in regulating cell sensitivity probably through the interaction with intra-HSPA family members, inter-HSP family members, and other families. The degree of cell sensitivity to acRoots increased in both sensitive and less-sensitive cells after deletion of HSPA6 genes. Thus, our data indicate that HSPA6 and HSPA6-dominated molecular network can be an alternative to modify cell sensitivity to drugs.
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the root of Actinidia chinensis inhibits hepatocellular carcinomas cells through lamb3
Cell Biology and Toxicology, 2018Co-Authors: Jiayun Hou, Lingyan WangAbstract:The root of Actinidia chinensis, as traditional Chinese medicine, has been shown to inhibit cell proliferation in numerous cancer cells. However, the mechanisms underlying its inhibitory activity remain unclear. Death rates of hepatocellular carcinoma (HCC) are increasing, but therapies for advanced HCC are not well developed. We choose the extract from root of Actinidia chinensis (ERAC) to treat the HCC cell lines in vitro, displaying distinct effects on cell proliferation, S-phase cell cycle arrest, and apoptosis. LAMB3, the gene encoding laminin subunit beta-3, plays a key role in the proliferation suppression and S-phase cell cycle arrest of HepG2 cells treated with ERAC. The downstream genes ITGA3, CCND2, and TP53 in LAMB3 pathway show the same response to ERAC as LAMB3. Thus, LAMB3 pathways, along with extracellular matrix-receptor interaction, pathways in cancer, and focal adhesion, are involved in the ERAC-induced suppressive response in HepG2.
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regulatory roles of oasl in lung cancer cell sensitivity to Actinidia chinensis planch root extract acroots
Cell Biology and Toxicology, 2018Co-Authors: Lingyan Wang, Xiangdong Wang, Hui ShenAbstract:Lung cancer is one of the most common malignancies worldwide. Actinidia chinensis Planch root extract (acRoots) was found to have the capacity of the anti-tumor, although the molecular mechanisms remain unclear. The present study aims to investigate the molecular mechanisms by which lung cancer cells sense to inhibitory effects of acRoots with a special focus on immune-associated gene profiles. We firstly provide a preclinical evidence that acRoots can significantly inhibit lung cancer cell proliferation and apoptosis via the PI3K-OASL signal pathway. The heterogeneous alterations of immune-associated gene profiles of lung cancer cell types were measured after treatment with various doses of acRoots. The OASL gene was identified as the key regulator in molecular networks of acRoots-treated lung cancer cells and validated. The OASL gene plays an important role in the regulation of lung cancer cell sensitivity to acRoots, which modulated by the PI3K signal pathway. Thus, our data indicate that OASL can be one of the decisive regulators to maintain lung cancer cell susceptibility to acRoots and may be associated with the development of drug resistance. The regulation of OASL can be an alternative strategy to improve drug efficacy during cancer therapies.
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Actinidia chinensis planch root extract inhibits cholesterol metabolism in hepatocellular carcinoma through upregulation of pcsk9
Oncotarget, 2017Co-Authors: Jiayun Hou, Lingyan Wang, Minghuan Zheng, Tingting Fang, Xiangdong Wang, Jinglin XiaAbstract:// Mingyan He 1, * , Jiayun Hou 2, * , Lingyan Wang 2, * , Minghuan Zheng 2 , Tingting Fang 1 , Xiangdong Wang 2 and Jinglin Xia 1, 3 1 Liver Cancer Institute, Zhongshan Hospital, Fudan University, Shanghai, China 2 Clinical Science Institute, Zhongshan Hospital, Fudan University, Shanghai, China 3 Minhang Hospital, Fudan University, Shanghai, China * These authors have contributed equally to this work Correspondence to: Jinglin Xia, email: xiajinglin@fudan.edu.cn Xiangdong Wang, email: xianagdong.wang@clintransmed.org Keywords: hepatocellular carcinoma, root of Actinidia chinensis, PCSK9, cholesterol metabolism Received: September 09, 2016 Accepted: January 16, 2017 Published: February 02, 2017 ABSTRACT Actinidia chinensis Planch root extract (acRoots) is a traditional Chinese medicine with anti-tumor efficacy. To investigate the mechanisms responsible for this activity, we examined the effects of acRoots on cholesterol metabolism in hepatocellular carcinoma (HCC). mRNA chip analysis was used to identify the metabolic genes regulated by acRoots. The effects of acRoots on cholesterol synthesis and uptake were evaluated by measuring intracellular cholesterol levels and 3,3’-dioctadecylindocarbocyanine-labeled low-density lipoprotein (Dil-LDL) uptake. Expression of metabolic genes was analyzed using quantitative reverse transcription PCR, western blotting, and flow cytometry. acRoots reduced the viability of LM3 and HepG2 cells at 5 mg/mL and HL-7702 cells at 30 mg/mL. Gene expression profiling revealed that treatment with acRoots altered expression of genes involved in immune responses, inflammation, proliferation, cell cycle control, and metabolism. We also confirmed that acRoots enhances expression of PCSK9 , which is important for cholesterol metabolism. This resulted in decreased LDL receptor expression, inhibition of LDL uptake by LM3 cells, decreased total intracellular cholesterol, and reduced proliferation. These effects were promoted by PCSK9 overexpression and rescued by PCSK9 knockdown. Our data demonstrate that acRoots is a novel anti-tumor agent that inhibits cholesterol metabolism though a PCSK9 -mediated signaling pathway.
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Actinidia chinensis planch root extract acroots inhibits hepatocellular carcinoma progression by inhibiting ep3 expression
Cell Biology and Toxicology, 2016Co-Authors: Tingting Fang, Jiayun Hou, Lingyan Wang, Minghuan Zheng, Xiangdong Wang, Jinglin XiaAbstract:A wide range of studies has demonstrated the potent anticancer activity of Chinese herbs. Here, we evaluated the anticancer activity and molecular mechanisms of Actinidia chinensis root extract (acRoots) on hepatocellular carcinoma (HCC). HepG2 HCC cells were treated with various concentrations of acRoots for 72 h and examined by mRNA expression profiling, revealing alterations in cellular immunity, inflammation, proliferation, cell cycle, and metabolic signaling responses. Further analysis of the altered genes in cellular immunity and inflammation gene clusters identified prostaglandin E receptor 3 (EP3) as a key regulator of gene expression in response to acRoots. Further analysis revealed inhibition of cell growth, migration, and invasion in HCC in response to acRoots, along with increased apoptosis due to downregulation of EP3 expression. Treatment with acRoots and EP3 antagonist L-798106 led to decreases in VEGF, EGFR, MMP2, and MMP9 expression in HCC cells, along with significant effects on growth, migration, invasion, and apoptosis; the effects were reversed/blocked by the EP3 agonist sulprostone. Taken together, these data clearly demonstrated that acRoots inhibit HCC cell invasion and metastasis via inhibition of EP3 expression, resulting in decreased activation of VEGF, EGFR, MMP2, and MMP9.
Hongwen Huang - One of the best experts on this subject based on the ideXlab platform.
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gene expression profiling of development and anthocyanin accumulation in kiwifruit Actinidia chinensis based on transcriptome sequencing
PLOS ONE, 2015Co-Authors: Yifei Liu, Shaohua Zeng, Gong Xiao, Gan Wang, Ying Wang, Ming Peng, Hongwen HuangAbstract:Red-fleshed kiwifruit (Actinidia chinensis Planch. ‘Hongyang’) is a promising commercial cultivar due to its nutritious value and unique flesh color, derived from vitamin C and anthocyanins. In this study, we obtained transcriptome data of ‘Hongyang’ from seven developmental stages using Illumina sequencing. We mapped 39–54 million reads to the recently sequenced kiwifruit genome and other databases to define gene structure, to analyze alternative splicing, and to quantify gene transcript abundance at different developmental stages. The transcript profiles throughout red kiwifruit development were constructed and analyzed, with a focus on the biosynthesis and metabolism of compounds such as phytohormones, sugars, starch and L-ascorbic acid, which are indispensable for the development and formation of quality fruit. Candidate genes for these pathways were identified through MapMan and phylogenetic analysis. The transcript levels of genes involved in sucrose and starch metabolism were consistent with the change in soluble sugar and starch content throughout kiwifruit development. The metabolism of L-ascorbic acid was very active, primarily through the L-galactose pathway. The genes responsible for the accumulation of anthocyanin in red kiwifruit were identified, and their expression levels were investigated during kiwifruit development. This survey of gene expression during kiwifruit development paves the way for further investigation of the development of this uniquely colored and nutritious fruit and reveals which factors are needed for high quality fruit formation. This transcriptome data and its analysis will be useful for improving kiwifruit genome annotation, for basic fruit molecular biology research, and for kiwifruit breeding and improvement.
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the first complete chloroplast genome sequences in Actinidiaceae genome structure and comparative analysis
PLOS ONE, 2015Co-Authors: Ping Tang, Zuo-zhou Li, Dawei Li, Hongwen HuangAbstract:Actinidia chinensis is an important economic plant belonging to the basal lineage of the asterids. Availability of a complete Actinidia chloroplast genome sequence is crucial to understanding phylogenetic relationships among major lineages of angiosperms and facilitates kiwifruit genetic improvement. We report here the complete nucleotide sequences of the chloroplast genomes for Actinidia chinensis and A. chinensis var deliciosa obtained through de novo assembly of Illumina paired-end reads produced by total DNA sequencing. The total genome size ranges from 155,446 to 157,557 bp, with an inverted repeat (IR) of 24,013 to 24,391 bp, a large single copy region (LSC) of 87,984 to 88,337 bp and a small single copy region (SSC) of 20,332 to 20,336 bp. The genome encodes 113 different genes, including 79 unique protein-coding genes, 30 tRNA genes and 4 ribosomal RNA genes, with 16 duplicated in the inverted repeats, and a tRNA gene (trnfM-CAU) duplicated once in the LSC region. Comparisons of IR boundaries among four asterid species showed that IR/LSC borders were extended into the 5’ portion of the psbA gene and IR contraction occurred in Actinidia. The clap gene has been lost from the chloroplast genome in Actinidia, and may have been transferred to the nucleus during chloroplast evolution. Twenty-seven polymorphic simple sequence repeat (SSR) loci were identified in the Actinidia chloroplast genome. Maximum parsimony analyses of a 72-gene, 16 taxa angiosperm dataset strongly support the placement of Actinidiaceae in Ericales within the basal asterids.
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geographical distribution and morphological diversity of red fleshed kiwifruit germplasm Actinidia chinensis planchon in china
Genetic Resources and Crop Evolution, 2013Co-Authors: Liyun Sui, Caihong Zhong, Yifei Liu, Hongwen HuangAbstract:The fruit flesh color of kiwifruit (Actinidia chinensis) is generally green or yellow when ripened. Developing kiwifruit cultivars with new fruit flesh color such as red-fleshed color has stimulated much interest for kiwifruit breeders and researchers recently due to its potentially importance for meeting the increasing and changing markets and consumers. In the present study, the whole geographical distribution and morphological variation of wild red-fleshed kiwifruit in China were investigated. In total of 56 accessions of red-fleshed kiwifruit were found across 19 separated localities, representing different ecological and climatic conditions throughout South and Central China. Characterization of nine horticulturally important fruit traits of all accessions showed that there are extensive variations in fruit shape, fruit hairs and as to red intensity, spread of the red pigments and background flesh color. The results presented here have updated the current knowledge on the natural distribution and ecological adaptation of wild red-fleshed kiwifruit resource, which should be valuable for kiwifruit agriculture such as the determination of planting areas of red-fleshed kiwifruit cultivars. The accessions with extensive morphological variation can also contribute to kiwifruit breeding in future such as developing new cultivars with red color flesh.
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characterization of 15 novel single nucleotide polymorphisms snps in the Actinidia chinensis species complex Actinidiaceae
American Journal of Botany, 2011Co-Authors: Jin Zhou, Yifei Liu, Hongwen HuangAbstract:PREMISE OF THE STUDY: Based on the ESTs database, we report here for the first time 15 polymorphic single nucleotide polymorphisms (SNPs) in the Actinidia chinensis species complex that can be used to study population genetic variation. METHODS AND RESULTS: SNPs derived from ESTs of the A. chinensis species complex were identified by the in silico SNP discovery strategy. The Cleaved Amplified polymorphic Sequences (CAPS) method was used to validate putative SNPs in a sample of 24 diploid A. chinensis individuals. For the 15 nuclear SNPs identified, all loci had two alleles, and the minor allele frequency ranged from 0.146 to 0.479. The expected and observed heterozygosities ranged from 0 to 0.500 and from 0 to 0.875, respectively. CONCLUSIONS: The developed markers in this study will provide valuable tools for characterizing genetic variation in evolutionary and ecological studies of natural populations.
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morphological and cytotype variation of wild kiwifruit Actinidia chinensis complex along an altitudinal and longitudinal gradient in central west china
Botanical Journal of the Linnean Society, 2010Co-Authors: Dawei Li, Caihong Zhong, Hongwen HuangAbstract:The Actinidia chinensis complex, a group of commercially important fruits (kiwifruit), is a complex of functionally dioecious lianas of variable ploidy. To understand the cytogeography better and to facilitate breeding in this complex, we examined the ploidy and morphological variations in 16 natural populations of A. chinensis var. chinensis and A. chinensis var. deliciosa across an ecogeographical gradient. Four ploidy levels were found, var. chinensis consisting of diploids and tetraploids and var. deliciosa consisting of tetraploids, pentaploids and hexaploids. Hexaploids were centred in the western Yun-Gui plateau, tetraploids coexisted with hexaploids or diploids in the middle Yun-Gui plateau and the Wuling-Xuefeng mountains, and diploids occurred in the eastern Wuling-Xuefeng mountains and the Hunan foothills. These findings indicate a gradual, clinal transition from hexaploid to diploid across the elevational and longitudinal gradient. The clear geographical segregation of diploids and hexaploids may have arisen from their differential ecological adaptation in response to altitude and climate, whereas the coexistence of cytotypes (2x–4x, 4x–6x and 4x–5x–6x) might be a result of reproductive barriers, with a particular contribution from the postzygotic reproductive isolation between ploidy races. The geographical pattern and morphological variation of cytotypes suggest a hybrid zone between the varieties in the Wuling-Xuefeng mountains. The differences in cytotypes which have arisen as a result of ecological adaptation, distribution and morphological characteristics will provide important baseline data for the selection of germplasm and the breeding of kiwifruit. © 2010 The Linnean Society of London, Botanical Journal of the Linnean Society, 2010, 164, 72–83.
Ross A Ferguson - One of the best experts on this subject based on the ideXlab platform.
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induced polyploidy dramatically increases the size and alters the shape of fruit in Actinidia chinensis
Annals of Botany, 2012Co-Authors: Ross A Ferguson, B G Murray, P M Datson, Yilin Jia, Jingli ZhangAbstract:† Background and Aims Some otherwise promising selections of Actinidia chinensis (kiwifruit) have fruit that are too small for successful commercialization. We have therefore made the first detailed study in diploid kiwifruit of the effects of chromosome doubling induced by colchicine on fruit size, shape and crop loading. † Methods Flow cytometric analysis of young leaves and chromosome analysis of flower buds and root tips was used to confirm the stability of induced autotetraploids. Fruit weight, size and crop load were measured in the third year after planting in the field and for three consecutive years. DNA fingerprinting was used to confirm the origin of the material. † Key Results There was a very significant increase in fruit size in induced autotetraploids of different genotypes of A. chinensis. With the commercially important diploid cultivar ‘Hort16A’, most regenerants, Type A plants, had fruit which were much the same shape as fruit of the diploid but, at the same fruit load, were much larger and heavier. Some regenerants, Type B plants, produced fruit similar to ‘fasciated’ fruit. Fruit of the autotetraploids induced from three female red-fleshed A. chinensis selections were also 50 ‐60 % larger than fruit of their diploid progenitors. The main increase in fruit dimensions was in their diameters. These improved fruit characteristics were stable over several seasons. † Conclusions Chromosome doubling has been shown to increase significantly fruit size in autotetraploid A. chinensis, highlighting the considerable potential of this technique to produce new cultivars with fruit of adequate size. Other variants with differently shaped fruit were also produced but the genetic basis of this variation remains to be elucidated. Autoploids of other Actinidia species with commercial potential may also show improved fruit characteristics, opening up many new possibilities for commercial development.
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manipulation of ploidy for kiwifruit breeding in vitro chromosome doubling in diploid Actinidia chinensis planch
Plant Cell Tissue and Organ Culture, 2011Co-Authors: Ross A Ferguson, Jinhu Wu, B G MurrayAbstract:Tetraploid plants were induced by colchicine treatment of in vitro leaf petiole segment cultures of five diploid Actinidia chinensis Planch. genotypes, including the commercially important, yellow-fleshed cultivar ‘Hort16A’, three female selections with red-fleshed fruit and one male pollinizer. Petiole segments were incubated on a shoot regeneration medium for a period of 4 weeks, and subsequently microshoots were treated with 0.05 or 0.1% colchicine. About one-third of the regenerated shoots were tetraploid following 0.05% colchicine treatment, more than with 0.1% colchicine treatment. Similar rates of tetraploid induction were achieved with all the genotypes tested. The efficiency of induction of polyploidy depended on the interaction between the types of in vitro culture chosen and the concentration of colchicine used. There are no previous reports of colchicine being used so successfully to induce polyploidy in Actinidia.
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pigments in the fruit of red fleshed kiwifruit Actinidia chinensis and Actinidia deliciosa
Journal of Agricultural and Food Chemistry, 2005Co-Authors: Mirco Montefiori, Tony K Mcghie, G Costa, Ross A FergusonAbstract:Kiwifruit cultivars (Actinidia chinensis and A. deliciosa) generally have fruit with yellow or green flesh when ripe. A small number of genotypes also have red pigments, usually restricted to the inner pericarp but varying in intensity and in distribution within the fruit. Carotenoids, chorophylls, and anthocyanins were extracted from the fruit pericarp of such red-fleshed kiwifruit selections. Pigments were analyzed by HPLC and identified by comparison with authentic standards and by liquid chromatography−mass spectroscopy to obtain a tentative identification of the major anthocyanins in red-fleshed kiwifruit. The yellow and green colors of the outer fruit pericarp are due to different concentrations and proportions of carotenoids and chlorophylls. The red color found mainly in the inner pericarp is due to anthocyanins. In the A. chinensis genotypes tested the major anthocyanin was cyanidin 3-O-xylo(1-2)-galactoside, with smaller amounts of cyanidin 3-O-galactoside. In the A. deliciosa genotypes analyzed...
