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Actinobacillus pleuropneumoniae
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John H. E. Nash – One of the best experts on this subject based on the ideXlab platform.
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Microarray-based comparative genomic profiling of reference strains and selected Canadian field isolates of Actinobacillus pleuropneumoniae
BMC genomics, 2009Co-Authors: Julien Gouré, Vincent Deslandes, John H. E. Nash, James W. Coulton, Simon J. Foote, Anne B. Bouevitch, Wendy A Findlay, Janet I. Macinnes, Mario JacquesAbstract:Background
Actinobacillus pleuropneumoniae, the causative agent of porcine pleuropneumonia, is a highly contagious respiratory pathogen that causes severe losses to the swine industry worldwide. Current commercially-available vaccines are of limited value because they do not induce cross-serovar immunity and do not prevent development of the carrier state. Microarray-based comparative genomic hybridizations (M-CGH) were used to estimate whole genomic diversity of representative Actinobacillus pleuropneumoniae strains. Our goal was to identify conserved genes, especially those predicted to encode outer membrane proteins and lipoproteins because of their potential for the development of more effective vaccines. -
multiplex pcr that can distinguish between immunologically cross reactive serovar 3 6 and 8 Actinobacillus pleuropneumoniae strains
Journal of Clinical Microbiology, 2008Co-Authors: L Zhou, Huanchun Chen, Janine T. Bossé, Sophie Jones, J S Kroll, Joachim Frey, John H. E. Nash, Øystein Angen, R Zhou, Andrew N. RycroftAbstract:We describe a highly sensitive and specific multiplex PCR, based on capsular loci and the species specific apxIV gene, that unequivocally differentiates serovar 3, 6, and 8 Actinobacillus pleuropneumoniae strains that are cross-reactive in conventional immunological tests.
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The Complete Genome Sequence of Actinobacillus pleuropneumoniae L20 (Serotype 5b)
Journal of bacteriology, 2007Co-Authors: Simon J. Foote, Paul R. Langford, Janine T. Bossé, Anne B. Bouevitch, N. Martin Young, John H. E. NashAbstract:There are 16 capsule-based serotypes of Actinobacillus pleuropneumoniae, all of which are capable of causing disease in pigs. Here we report the finished and annotated genome sequence of the reference serotype 5b strain L20. This strain has a rough appearance and readily forms biofilms, as is typical for most field isolates (6).
Janine T. Bossé – One of the best experts on this subject based on the ideXlab platform.
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complete genome sequence of midg2331 a genetically tractable serovar 8 clinical isolate of Actinobacillus pleuropneumoniae
Genome Announcements, 2016Co-Authors: Janine T. Bossé, Duncan J Maskell, Roy R Chaudhuri, Matthew T G Holden, Leon G Leanse, Roberto Fernandez Crespo, Paul Coupland, Denise Mara Soares Bazzolli, Alexander W Tucker, Brendan W WrenAbstract:We report here the complete annotated genome sequence of a clinical serovar 8 isolate Actinobacillus pleuropneumoniae MIDG2331. Unlike the serovar 8 reference strain 405, MIDG2331 is amenable to genetic manipulation via natural transformation as well as conjugation, making it ideal for studies of gene function.
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characterisation of a mobilisable plasmid conferring florfenicol and chloramphenicol resistance in Actinobacillus pleuropneumoniae
Veterinary Microbiology, 2015Co-Authors: Janine T. Bossé, Yanwen Li, Tom G Atherton, Stephanie Walker, Susanna Williamson, Jon Rogers, Roy R Chaudhuri, Lucy A Weinert, Matthew T G Holden, Duncan J MaskellAbstract:The complete nucleotide sequence of a 7.7 kb mobilisable plasmid (pM3446F), isolated from a florfenicol resistant isolate of Actinobacillus pleuropneumoniae, showed extended similarity to plasmids found in other members of the Pasteurellaceae containing the floR gene as well as replication and mobilisation genes. Mobilisation into other Pasteurellaceae species confirmed that this plasmid can be transferred horizontally.
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Prevalence of Actinobacillus pleuropneumoniae serovars in England and Wales
The Veterinary record, 2010Co-Authors: Ciaragh O’neill, Andrew N. Rycroft, Janine T. Bossé, Sophie Jones, Conrad M. Watson, S. M. Williamson, J S Kroll, Helen M. Hartley, Paul R. LangfordAbstract:Actinobacillus pleuropneumoniae is the causative agent of porcine pleuropneumonia, an economically important endemic bacterial disease of pigs worldwide ([Bosse and others 2002][1]). Epidemiologically, serotyping is the gold standard method, with A pleuropneumoniae being classified into 16
Øystein Angen – One of the best experts on this subject based on the ideXlab platform.
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Draft genome sequences of Actinobacillus pleuropneumoniae serotypes 2 and 6.
Journal of bacteriology, 2010Co-Authors: Bujie Zhan, Øystein Angen, Jakob Hedegaard, Christian Bendixen, Frank PanitzAbstract:Actinobacillus pleuropneumoniae is a bacterial pathogen that causes highly contagious respiratory infection in pigs and has a serious impact on the production economy and animal welfare. As clear differences in virulence between serotypes have been observed, the genetic basis should be investigated at the genomic level. Here, we present the draft genome sequences of the A. pleuropneumoniae serotypes 2 (strain 4226) and 6 (strain Femo).
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multiplex pcr that can distinguish between immunologically cross reactive serovar 3 6 and 8 Actinobacillus pleuropneumoniae strains
Journal of Clinical Microbiology, 2008Co-Authors: L Zhou, Huanchun Chen, Janine T. Bossé, Sophie Jones, J S Kroll, Joachim Frey, John H. E. Nash, Øystein Angen, R Zhou, Andrew N. RycroftAbstract:We describe a highly sensitive and specific multiplex PCR, based on capsular loci and the species specific apxIV gene, that unequivocally differentiates serovar 3, 6, and 8 Actinobacillus pleuropneumoniae strains that are cross-reactive in conventional immunological tests.
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detection and identification of Actinobacillus pleuropneumoniae serotypes 1 2 and 8 by multiplex pcr
Journal of Clinical Microbiology, 2004Co-Authors: Jennifer A Schuchert, Øystein Angen, Thomas J Inzana, Stine Graakjaer JessingAbstract:Multiplex PCR assays were developed to identify Actinobacillus pleuropneumoniae serotypes 1, 2, and 8. Primers designed for the conserved capsular polysaccharide (CP) export region amplified a 489-bp DNA fragment from all serotypes. Primers specific to the CP biosynthesis regions of serotypes 1, 2, and 8 amplified fragments of 1.6 kb, 1.7 kb, and 970 bp from only their respective serotypes.