The Experts below are selected from a list of 5580 Experts worldwide ranked by ideXlab platform
John H. E. Nash - One of the best experts on this subject based on the ideXlab platform.
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Microarray-based comparative genomic profiling of reference strains and selected Canadian field isolates of Actinobacillus pleuropneumoniae
BMC genomics, 2009Co-Authors: Julien Gouré, Vincent Deslandes, John H. E. Nash, James W. Coulton, Simon J. Foote, Anne B. Bouevitch, Wendy A Findlay, Janet I. Macinnes, Mario JacquesAbstract:Background Actinobacillus pleuropneumoniae, the causative agent of porcine pleuropneumonia, is a highly contagious respiratory pathogen that causes severe losses to the swine industry worldwide. Current commercially-available vaccines are of limited value because they do not induce cross-serovar immunity and do not prevent development of the carrier state. Microarray-based comparative genomic hybridizations (M-CGH) were used to estimate whole genomic diversity of representative Actinobacillus pleuropneumoniae strains. Our goal was to identify conserved genes, especially those predicted to encode outer membrane proteins and lipoproteins because of their potential for the development of more effective vaccines.
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multiplex pcr that can distinguish between immunologically cross reactive serovar 3 6 and 8 Actinobacillus pleuropneumoniae strains
Journal of Clinical Microbiology, 2008Co-Authors: L Zhou, Huanchun Chen, Janine T. Bossé, Sophie Jones, J S Kroll, Joachim Frey, John H. E. Nash, Øystein Angen, R Zhou, Andrew N. RycroftAbstract:We describe a highly sensitive and specific multiplex PCR, based on capsular loci and the species specific apxIV gene, that unequivocally differentiates serovar 3, 6, and 8 Actinobacillus pleuropneumoniae strains that are cross-reactive in conventional immunological tests.
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The Complete Genome Sequence of Actinobacillus pleuropneumoniae L20 (Serotype 5b)
Journal of bacteriology, 2007Co-Authors: Simon J. Foote, Paul R. Langford, Janine T. Bossé, Anne B. Bouevitch, N. Martin Young, John H. E. NashAbstract:There are 16 capsule-based serotypes of Actinobacillus pleuropneumoniae, all of which are capable of causing disease in pigs. Here we report the finished and annotated genome sequence of the reference serotype 5b strain L20. This strain has a rough appearance and readily forms biofilms, as is typical for most field isolates (6).
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Transcriptional profiling of Actinobacillus pleuropneumoniae under iron-restricted conditions
BMC genomics, 2007Co-Authors: Vincent Deslandes, John H. E. Nash, Josée Harel, James W. Coulton, Mario JacquesAbstract:Background To better understand effects of iron restriction on Actinobacillus pleuropneumoniae and to identify new potential vaccine targets, we conducted transcript profiling studies using a DNA microarray containing all 2025 ORFs of the genome of A. pleuropneumoniae serotype 5b strain L20. This is the first study involving the use of microarray technology to monitor the transcriptome of A. pleuropneumoniae grown under iron restriction.
Janine T. Bossé - One of the best experts on this subject based on the ideXlab platform.
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complete genome sequence of midg2331 a genetically tractable serovar 8 clinical isolate of Actinobacillus pleuropneumoniae
Genome Announcements, 2016Co-Authors: Janine T. Bossé, Roy R Chaudhuri, Matthew T G Holden, Duncan J Maskell, Leon G Leanse, Roberto Fernandez Crespo, Paul Coupland, Denise Mara Soares Bazzolli, Alexander W Tucker, Brendan W WrenAbstract:We report here the complete annotated genome sequence of a clinical serovar 8 isolate Actinobacillus pleuropneumoniae MIDG2331. Unlike the serovar 8 reference strain 405, MIDG2331 is amenable to genetic manipulation via natural transformation as well as conjugation, making it ideal for studies of gene function.
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characterisation of a mobilisable plasmid conferring florfenicol and chloramphenicol resistance in Actinobacillus pleuropneumoniae
Veterinary Microbiology, 2015Co-Authors: Janine T. Bossé, Yanwen Li, Tom G Atherton, Stephanie Walker, Susanna Williamson, Jon Rogers, Roy R Chaudhuri, Lucy A Weinert, Matthew T G Holden, Duncan J MaskellAbstract:The complete nucleotide sequence of a 7.7 kb mobilisable plasmid (pM3446F), isolated from a florfenicol resistant isolate of Actinobacillus pleuropneumoniae, showed extended similarity to plasmids found in other members of the Pasteurellaceae containing the floR gene as well as replication and mobilisation genes. Mobilisation into other Pasteurellaceae species confirmed that this plasmid can be transferred horizontally.
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Prevalence of Actinobacillus pleuropneumoniae serovars in England and Wales
The Veterinary record, 2010Co-Authors: Ciaragh O’neill, Andrew N. Rycroft, Janine T. Bossé, Sophie Jones, Conrad M. Watson, S. M. Williamson, J S Kroll, Helen M. Hartley, Paul R. LangfordAbstract:Actinobacillus pleuropneumoniae is the causative agent of porcine pleuropneumonia, an economically important endemic bacterial disease of pigs worldwide ([Bosse and others 2002][1]). Epidemiologically, serotyping is the gold standard method, with A pleuropneumoniae being classified into 16
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Natural competence in strains of Actinobacillus pleuropneumoniae.
FEMS microbiology letters, 2009Co-Authors: Janine T. Bossé, J. Simon Kroll, Sunita Sinha, Timo Schippers, Rosemary J. Redfield, Paul R. LangfordAbstract:We have identified a highly transformable strain of Actinobacillus pleuropneumoniae whose competence is regulated by the competence-activator Sxy as in other Pasteurellaceae. Other strains were poorly transformable or nontransformable. The genomes of two poorly transformable strains contain intact sets of competence genes. Moreover, we show that the low competence of one of these strains is not due to an inability to induce sxy expression or to a defect in Sxy function, suggesting that some other component of the competence system is defective. Although the A. pleuropneumoniae sxy gene has only 24% identity to its Haemophilus influenzae homologue, both genes fully complemented an H. influenzae sxy knockout, demonstrating that Sxy function is conserved throughout the Pasteurellaceae.
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multiplex pcr that can distinguish between immunologically cross reactive serovar 3 6 and 8 Actinobacillus pleuropneumoniae strains
Journal of Clinical Microbiology, 2008Co-Authors: L Zhou, Huanchun Chen, Janine T. Bossé, Sophie Jones, J S Kroll, Joachim Frey, John H. E. Nash, Øystein Angen, R Zhou, Andrew N. RycroftAbstract:We describe a highly sensitive and specific multiplex PCR, based on capsular loci and the species specific apxIV gene, that unequivocally differentiates serovar 3, 6, and 8 Actinobacillus pleuropneumoniae strains that are cross-reactive in conventional immunological tests.
Øystein Angen - One of the best experts on this subject based on the ideXlab platform.
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Draft genome sequences of Actinobacillus pleuropneumoniae serotypes 2 and 6.
Journal of bacteriology, 2010Co-Authors: Bujie Zhan, Øystein Angen, Jakob Hedegaard, Christian Bendixen, Frank PanitzAbstract:Actinobacillus pleuropneumoniae is a bacterial pathogen that causes highly contagious respiratory infection in pigs and has a serious impact on the production economy and animal welfare. As clear differences in virulence between serotypes have been observed, the genetic basis should be investigated at the genomic level. Here, we present the draft genome sequences of the A. pleuropneumoniae serotypes 2 (strain 4226) and 6 (strain Femo).
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multiplex pcr that can distinguish between immunologically cross reactive serovar 3 6 and 8 Actinobacillus pleuropneumoniae strains
Journal of Clinical Microbiology, 2008Co-Authors: L Zhou, Huanchun Chen, Janine T. Bossé, Sophie Jones, J S Kroll, Joachim Frey, John H. E. Nash, Øystein Angen, R Zhou, Andrew N. RycroftAbstract:We describe a highly sensitive and specific multiplex PCR, based on capsular loci and the species specific apxIV gene, that unequivocally differentiates serovar 3, 6, and 8 Actinobacillus pleuropneumoniae strains that are cross-reactive in conventional immunological tests.
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detection and identification of Actinobacillus pleuropneumoniae serotypes 1 2 and 8 by multiplex pcr
Journal of Clinical Microbiology, 2004Co-Authors: Jennifer A Schuchert, Øystein Angen, Thomas J Inzana, Stine Graakjaer JessingAbstract:Multiplex PCR assays were developed to identify Actinobacillus pleuropneumoniae serotypes 1, 2, and 8. Primers designed for the conserved capsular polysaccharide (CP) export region amplified a 489-bp DNA fragment from all serotypes. Primers specific to the CP biosynthesis regions of serotypes 1, 2, and 8 amplified fragments of 1.6 kb, 1.7 kb, and 970 bp from only their respective serotypes.
Paul R. Langford - One of the best experts on this subject based on the ideXlab platform.
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necrosis from needlestick injury with live Actinobacillus pleuropneumoniae porcine vaccine
BMJ, 2011Co-Authors: Andrew N. Rycroft, Pornchalit Assavacheep, Michael Jacobs, Paul R. LangfordAbstract:Actinobacillus pleuropneumoniae causes porcine pleuropneumonia, a highly infectious and commonly lethal disease in pigs.1 It is present in porcine respiratory tract wherever pig production is industrialised, but it is not considered to be zoonotic.2 To our knowledge, no cases of infection have been reported in animals other than pigs or in humans, including abattoir and farm workers. Vaccinating pigs against pleuropneumonia is an important …
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Prevalence of Actinobacillus pleuropneumoniae serovars in England and Wales
The Veterinary record, 2010Co-Authors: Ciaragh O’neill, Andrew N. Rycroft, Janine T. Bossé, Sophie Jones, Conrad M. Watson, S. M. Williamson, J S Kroll, Helen M. Hartley, Paul R. LangfordAbstract:Actinobacillus pleuropneumoniae is the causative agent of porcine pleuropneumonia, an economically important endemic bacterial disease of pigs worldwide ([Bosse and others 2002][1]). Epidemiologically, serotyping is the gold standard method, with A pleuropneumoniae being classified into 16
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Natural competence in strains of Actinobacillus pleuropneumoniae.
FEMS microbiology letters, 2009Co-Authors: Janine T. Bossé, J. Simon Kroll, Sunita Sinha, Timo Schippers, Rosemary J. Redfield, Paul R. LangfordAbstract:We have identified a highly transformable strain of Actinobacillus pleuropneumoniae whose competence is regulated by the competence-activator Sxy as in other Pasteurellaceae. Other strains were poorly transformable or nontransformable. The genomes of two poorly transformable strains contain intact sets of competence genes. Moreover, we show that the low competence of one of these strains is not due to an inability to induce sxy expression or to a defect in Sxy function, suggesting that some other component of the competence system is defective. Although the A. pleuropneumoniae sxy gene has only 24% identity to its Haemophilus influenzae homologue, both genes fully complemented an H. influenzae sxy knockout, demonstrating that Sxy function is conserved throughout the Pasteurellaceae.
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The Complete Genome Sequence of Actinobacillus pleuropneumoniae L20 (Serotype 5b)
Journal of bacteriology, 2007Co-Authors: Simon J. Foote, Paul R. Langford, Janine T. Bossé, Anne B. Bouevitch, N. Martin Young, John H. E. NashAbstract:There are 16 capsule-based serotypes of Actinobacillus pleuropneumoniae, all of which are capable of causing disease in pigs. Here we report the finished and annotated genome sequence of the reference serotype 5b strain L20. This strain has a rough appearance and readily forms biofilms, as is typical for most field isolates (6).
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Actinobacillus pleuropneumoniae: pathobiology and pathogenesis of infection.
Microbes and infection, 2002Co-Authors: Janine T. Bossé, Andrew N. Rycroft, Håkan Janson, Brian J. Sheehan, Amanda Beddek, J. Simon Kroll, Paul R. LangfordAbstract:Actinobacillus pleuropneumoniae causes porcine pleuropneumonia, a highly contagious disease for which there is no effective vaccine. This review considers how adhesins, iron-acquisition factors, capsule and lipopolysaccharide, RTX cytotoxins and other potential future vaccine components contribute to colonisation, to avoidance of host clearance mechanisms and to damage of host tissues.
Stine Graakjaer Jessing - One of the best experts on this subject based on the ideXlab platform.
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detection and identification of Actinobacillus pleuropneumoniae serotypes 1 2 and 8 by multiplex pcr
Journal of Clinical Microbiology, 2004Co-Authors: Jennifer A Schuchert, Øystein Angen, Thomas J Inzana, Stine Graakjaer JessingAbstract:Multiplex PCR assays were developed to identify Actinobacillus pleuropneumoniae serotypes 1, 2, and 8. Primers designed for the conserved capsular polysaccharide (CP) export region amplified a 489-bp DNA fragment from all serotypes. Primers specific to the CP biosynthesis regions of serotypes 1, 2, and 8 amplified fragments of 1.6 kb, 1.7 kb, and 970 bp from only their respective serotypes.