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Sergio Svistoonoff - One of the best experts on this subject based on the ideXlab platform.
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Molecular Methods for Research on Actinorhiza
HAL CCSD, 2019Co-Authors: Hassen Gherbi, Mariama Ngom, Valérie Hocher, Luis G. Wall, Alyssa Carré-mlouka, Louis S Tisa, Fournier Joëlle, Nathalie Diagne, Sergio SvistoonoffAbstract:International audienceActinorhizal root nodules result from the interaction between a nitrogen-fixing actinomycete from the genus Frankia and roots of dicotyledonous trees and shrubs belonging to 25 genera within 8 plant families. Most Actinorhizal plants can reach high rates of nitrogen fixation comparable to those found in root nodule symbiosis of the legumes. As a consequence, these trees are able to grow in poor and disturbed soils and are important elements in plant communities worldwide. While the basic knowledge of these symbiotic associations is still poorly understood, Actinorhizal symbioses emerged recently as original systems to explore developmental strategies to form nitrogen-fixing nodules. Many tools have been developed in recent years to explore the interaction between Frankia and Actinorhizal plants including molecular biology, biochemistry, and genomics. However, technical difficulties are often encountered to explore these symbiotic interactions, mainly linked to the woody nature of the plant species and to the lack of genetic tools for their bacterial symbionts. In this chapter, we report an inventory of the main recent molecular tools and techniques developed for studying Actinorhizae
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molecular methods for research on Actinorhiza
2019Co-Authors: Hassen Gherbi, Mariama Ngom, Valérie Hocher, Louis S Tisa, Luis Gabriel Wall, Joëlle Fournier, Nathalie Diagne, Alyssa Carremlouka, Sergio SvistoonoffAbstract:Actinorhizal root nodules result from the interaction between a nitrogen-fixing actinomycete from the genus Frankia and roots of dicotyledonous trees and shrubs belonging to 25 genera within 8 plant families. Most Actinorhizal plants can reach high rates of nitrogen fixation comparable to those found in root nodule symbiosis of the legumes. As a consequence, these trees are able to grow in poor and disturbed soils and are important elements in plant communities worldwide. While the basic knowledge of these symbiotic associations is still poorly understood, Actinorhizal symbioses emerged recently as original systems to explore developmental strategies to form nitrogen-fixing nodules. Many tools have been developed in recent years to explore the interaction between Frankia and Actinorhizal plants including molecular biology, biochemistry, and genomics. However, technical difficulties are often encountered to explore these symbiotic interactions, mainly linked to the woody nature of the plant species and to the lack of genetic tools for their bacterial symbionts. In this chapter, we report an inventory of the main recent molecular tools and techniques developed for studying Actinorhizae.
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Signalling in Actinorhizal root nodule symbioses
Antonie van Leeuwenhoek, 2019Co-Authors: Valérie Hocher, Mariama Ngom, Hassen Gherbi, Alyssa Carré-mlouka, Pierre Tisseyre, Sergio SvistoonoffAbstract:Plants able to establish a nitrogen-fixing root nodule symbiosis with the actinobacterium Frankia are called Actinorhizal. These interactions lead to the formation of new root organs, called Actinorhizal nodules, where the bacteria are hosted intracellularly and fix atmospheric nitrogen thus providing the plant with an almost unlimited source of nitrogen for its nutrition. Like other symbiotic interactions, Actinorhizal nodulation involves elaborate signalling between both partners of the symbiosis, leading to specific recognition between the plant and its compatible microbial partner, its accommodation inside plant cells and the development of functional root nodules. Actinorhizal nodulation shares many features with rhizobial nodulation but our knowledge on the molecular mechanisms involved in Actinorhizal nodulation remains very scarce. However recent technical achievements for several Actinorhizal species are allowing major discoveries in this field. In this review, we provide an outline on signalling molecules involved at different stages of Actinorhizal nodule formation and the corresponding signalling pathways and gene networks.
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The plant-growth-promoting actinobacteria of the genus Nocardia induces root nodule formation in Casuarina glauca
Antonie van Leeuwenhoek, 2019Co-Authors: Faten Ghodhbane-gtari, Laurent Laplaze, Hassen Gherbi, Imen Nouioui, Virginie Vaissayre, Karima Hezbri, Emily Lundstedt, Timothy D’angelo, Zakkary Mcnutt, Sergio SvistoonoffAbstract:Actinorhizal plants form a symbiotic association with the nitrogen-fixing actinobacteria Frankia. These plants have important economic and ecological benefits including land reclamation, soil stabilization, and reforestation. Recently, many non-Frankia actinobacteria have been isolated from Actinorhizal root nodules suggesting that they might contribute to nodulation. Two Nocardia strains, BMG51109 and BMG111209, were isolated from Casuarina glauca nodules, and they induced root nodule-like structures in original host plant promoting seedling growth. The formed root nodule-like structures lacked a nodular root at the apex, were not capable of reducing nitrogen and had their cortical cells occupied with rod-shaped Nocardiae cells. Both Nocardia strains induced root hair deformation on the host plant. BMG111209 strain induced the expression of the ProCgNin:Gus gene, a plant gene involved in the early steps of the infection process and nodulation development. Nocardia strain BMG51109 produced three types of auxins (Indole-3-acetic acid [IAA], Indole-3-Byturic Acid [IBA] and Phenyl Acetic Acid [PAA]), while Nocardia BMG111209 only produced IAA. Analysis of the Nocardia genomes identified several important predicted biosynthetic gene clusters for plant phytohormones, secondary metabolites, and novel natural products. Co-infection studies showed that Nocardia strain BMG51109 plays a role as a helper bacteria promoting an earlier onset of nodulation. This study raises many questions on the ecological significance and functionality of Nocardia bacteria in Actinorhizal symbioses.
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The Independent Acquisition of Plant Root Nitrogen- Fixing Symbiosis in Fabids Recruited the Same Genetic Pathway for Nodule Organogenesis
2016Co-Authors: Sergio Svistoonoff, Mathish Nambiar-veetil, Virginie Vaissayre, Faiza Meriem Benabdoun, Stella Cesari, Nathalie Diagne, Françoise De Billy, Jocelyne Bonneau, Ro Imanishi, Luis WallAbstract:Only species belonging to the Fabid clade, limited to four classes and ten families of Angiosperms, are able to form nitrogen-fixing root nodule symbioses (RNS) with soil bacteria. This concerns plants of the legume family (Fabaceae) and Parasponia (Cannabaceae) associated with the Gram-negative proteobacteria collectively called rhizobia and Actinorhizal plants associated with the Gram-positive actinomycetes of the genus Frankia. Calcium and calmodulin-dependent protein kinase (CCaMK) is a key component of the common signaling pathway leading to both rhizobial and arbuscular mycorrhizal symbioses (AM) and plays a central role in cross-signaling between root nodule organogenesis and infection processes. Here, we show that CCaMK is also needed for successful Actinorhiza formation and interaction with AM fungi in the Actinorhizal tree Casuarina glauca and is also able to restore both nodulation and AM symbioses in a Medicago truncatula ccamk mutant. Besides, we expressed auto-active CgCCaMK lacking the auto-inhibitory/CaM domain in two Actinorhizal species: C. glauca (Casuarinaceae), which develops an intracellular infection pathway, and Discaria trinervis (Rhamnaceae
Katharina Pawlowski - One of the best experts on this subject based on the ideXlab platform.
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comparative analysis of the nodule transcriptomes of ceanothus thyrsiflorus rhamnaceae rosales and datisca glomerata datiscaceae cucurbitales
Frontiers in Plant Science, 2018Co-Authors: Marco G. Salgado, Kai Battenberg, Alison M. Berry, Robin Van Velzen, Thanh Van Nguyen, Daniel Lundin, Katharina PawlowskiAbstract:Two types of nitrogen-fixing root nodule symbioses are known, rhizobial and Actinorhizal symbioses. The latter involve plants of three orders, Fagales, Rosales, and Cucurbitales. To understand the diversity of plant symbiotic adaptation, we compared the nodule transcriptomes of Datisca glomerata (Datiscaceae, Cucurbitales) and Ceanothus thyrsiflorus (Rhamnaceae, Rosales); both species are nodulated by members of the uncultured Frankia clade, cluster II. The analysis focused on various features. In both species, the expression of orthologs of legume Nod factor receptor genes was elevated in nodules compared to roots. Since arginine has been postulated as export form of fixed nitrogen from symbiotic Frankia in nodules of D. glomerata, the question was whether the nitrogen metabolism was similar in nodules of C. thyrsiflorus. Analysis of the expression levels of key genes encoding enzymes involved in arginine metabolism revealed up-regulation of arginine catabolism, but no up-regulation of arginine biosynthesis, in nodules compared to roots of D. glomerata, while arginine degradation was not upregulated in nodules of C. thyrsiflorus. This new information corroborated an arginine-based metabolic exchange between host and microsymbiont for D. glomerata, but not for C. thyrsiflorus. Oxygen protection systems for nitrogenase differ dramatically between both species. Analysis of the antioxidant system suggested that the system in the nodules of D. glomerata leads to greater oxidative stress than the one in the nodules of C. thyrsiflorus, while no differences were found for the defense against nitrosative stress. However, induction of nitrite reductase in nodules of C. thyrsiflorus indicated that here, nitrite produced from nitric oxide had to be detoxified. Additional shared features were identified: genes encoding enzymes involved in thiamine biosynthesis were found to be upregulated in the nodules of both species. Orthologous nodule-specific subtilisin-like proteases that have been linked to the infection process in Actinorhizal Fagales, were also upregulated in the nodules of D. glomerata and C. thyrsiflorus. Nodule-specific defensin genes known from Actinorhizal Fagales and Cucurbitales, were also found in C. thyrsiflorus. In summary, the results underline the variability of nodule metabolism in different groups of symbiotic plants while pointing at conserved features involved in the infection process.
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Image_4_Comparative Analysis of the Nodule Transcriptomes of Ceanothus thyrsiflorus (Rhamnaceae, Rosales) and Datisca glomerata (Datiscaceae, Cucurbitales).eps
2018Co-Authors: Marco G. Salgado, Kai Battenberg, Alison M. Berry, Robin Van Velzen, Thanh Van Nguyen, Daniel Lundin, Katharina PawlowskiAbstract:Two types of nitrogen-fixing root nodule symbioses are known, rhizobial and Actinorhizal symbioses. The latter involve plants of three orders, Fagales, Rosales, and Cucurbitales. To understand the diversity of plant symbiotic adaptation, we compared the nodule transcriptomes of Datisca glomerata (Datiscaceae, Cucurbitales) and Ceanothus thyrsiflorus (Rhamnaceae, Rosales); both species are nodulated by members of the uncultured Frankia clade, cluster II. The analysis focused on various features. In both species, the expression of orthologs of legume Nod factor receptor genes was elevated in nodules compared to roots. Since arginine has been postulated as export form of fixed nitrogen from symbiotic Frankia in nodules of D. glomerata, the question was whether the nitrogen metabolism was similar in nodules of C. thyrsiflorus. Analysis of the expression levels of key genes encoding enzymes involved in arginine metabolism revealed up-regulation of arginine catabolism, but no up-regulation of arginine biosynthesis, in nodules compared to roots of D. glomerata, while arginine degradation was not upregulated in nodules of C. thyrsiflorus. This new information corroborated an arginine-based metabolic exchange between host and microsymbiont for D. glomerata, but not for C. thyrsiflorus. Oxygen protection systems for nitrogenase differ dramatically between both species. Analysis of the antioxidant system suggested that the system in the nodules of D. glomerata leads to greater oxidative stress than the one in the nodules of C. thyrsiflorus, while no differences were found for the defense against nitrosative stress. However, induction of nitrite reductase in nodules of C. thyrsiflorus indicated that here, nitrite produced from nitric oxide had to be detoxified. Additional shared features were identified: genes encoding enzymes involved in thiamine biosynthesis were found to be upregulated in the nodules of both species. Orthologous nodule-specific subtilisin-like proteases that have been linked to the infection process in Actinorhizal Fagales, were also upregulated in the nodules of D. glomerata and C. thyrsiflorus. Nodule-specific defensin genes known from Actinorhizal Fagales and Cucurbitales, were also found in C. thyrsiflorus. In summary, the results underline the variability of nodule metabolism in different groups of symbiotic plants while pointing at conserved features involved in the infection process.
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Table_6_Comparative Analysis of the Nodule Transcriptomes of Ceanothus thyrsiflorus (Rhamnaceae, Rosales) and Datisca glomerata (Datiscaceae, Cucurbitales).xlsx
2018Co-Authors: Marco G. Salgado, Kai Battenberg, Alison M. Berry, Robin Van Velzen, Thanh Van Nguyen, Daniel Lundin, Katharina PawlowskiAbstract:Two types of nitrogen-fixing root nodule symbioses are known, rhizobial and Actinorhizal symbioses. The latter involve plants of three orders, Fagales, Rosales, and Cucurbitales. To understand the diversity of plant symbiotic adaptation, we compared the nodule transcriptomes of Datisca glomerata (Datiscaceae, Cucurbitales) and Ceanothus thyrsiflorus (Rhamnaceae, Rosales); both species are nodulated by members of the uncultured Frankia clade, cluster II. The analysis focused on various features. In both species, the expression of orthologs of legume Nod factor receptor genes was elevated in nodules compared to roots. Since arginine has been postulated as export form of fixed nitrogen from symbiotic Frankia in nodules of D. glomerata, the question was whether the nitrogen metabolism was similar in nodules of C. thyrsiflorus. Analysis of the expression levels of key genes encoding enzymes involved in arginine metabolism revealed up-regulation of arginine catabolism, but no up-regulation of arginine biosynthesis, in nodules compared to roots of D. glomerata, while arginine degradation was not upregulated in nodules of C. thyrsiflorus. This new information corroborated an arginine-based metabolic exchange between host and microsymbiont for D. glomerata, but not for C. thyrsiflorus. Oxygen protection systems for nitrogenase differ dramatically between both species. Analysis of the antioxidant system suggested that the system in the nodules of D. glomerata leads to greater oxidative stress than the one in the nodules of C. thyrsiflorus, while no differences were found for the defense against nitrosative stress. However, induction of nitrite reductase in nodules of C. thyrsiflorus indicated that here, nitrite produced from nitric oxide had to be detoxified. Additional shared features were identified: genes encoding enzymes involved in thiamine biosynthesis were found to be upregulated in the nodules of both species. Orthologous nodule-specific subtilisin-like proteases that have been linked to the infection process in Actinorhizal Fagales, were also upregulated in the nodules of D. glomerata and C. thyrsiflorus. Nodule-specific defensin genes known from Actinorhizal Fagales and Cucurbitales, were also found in C. thyrsiflorus. In summary, the results underline the variability of nodule metabolism in different groups of symbiotic plants while pointing at conserved features involved in the infection process.
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Image_10_Comparative Analysis of the Nodule Transcriptomes of Ceanothus thyrsiflorus (Rhamnaceae, Rosales) and Datisca glomerata (Datiscaceae, Cucurbitales).eps
2018Co-Authors: Marco G. Salgado, Kai Battenberg, Alison M. Berry, Robin Van Velzen, Thanh Van Nguyen, Daniel Lundin, Katharina PawlowskiAbstract:Two types of nitrogen-fixing root nodule symbioses are known, rhizobial and Actinorhizal symbioses. The latter involve plants of three orders, Fagales, Rosales, and Cucurbitales. To understand the diversity of plant symbiotic adaptation, we compared the nodule transcriptomes of Datisca glomerata (Datiscaceae, Cucurbitales) and Ceanothus thyrsiflorus (Rhamnaceae, Rosales); both species are nodulated by members of the uncultured Frankia clade, cluster II. The analysis focused on various features. In both species, the expression of orthologs of legume Nod factor receptor genes was elevated in nodules compared to roots. Since arginine has been postulated as export form of fixed nitrogen from symbiotic Frankia in nodules of D. glomerata, the question was whether the nitrogen metabolism was similar in nodules of C. thyrsiflorus. Analysis of the expression levels of key genes encoding enzymes involved in arginine metabolism revealed up-regulation of arginine catabolism, but no up-regulation of arginine biosynthesis, in nodules compared to roots of D. glomerata, while arginine degradation was not upregulated in nodules of C. thyrsiflorus. This new information corroborated an arginine-based metabolic exchange between host and microsymbiont for D. glomerata, but not for C. thyrsiflorus. Oxygen protection systems for nitrogenase differ dramatically between both species. Analysis of the antioxidant system suggested that the system in the nodules of D. glomerata leads to greater oxidative stress than the one in the nodules of C. thyrsiflorus, while no differences were found for the defense against nitrosative stress. However, induction of nitrite reductase in nodules of C. thyrsiflorus indicated that here, nitrite produced from nitric oxide had to be detoxified. Additional shared features were identified: genes encoding enzymes involved in thiamine biosynthesis were found to be upregulated in the nodules of both species. Orthologous nodule-specific subtilisin-like proteases that have been linked to the infection process in Actinorhizal Fagales, were also upregulated in the nodules of D. glomerata and C. thyrsiflorus. Nodule-specific defensin genes known from Actinorhizal Fagales and Cucurbitales, were also found in C. thyrsiflorus. In summary, the results underline the variability of nodule metabolism in different groups of symbiotic plants while pointing at conserved features involved in the infection process.
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the n metabolites of roots and Actinorhizal nodules from alnus glutinosa and datisca glomerata can d glomerata change n transport forms when nodulated
Symbiosis, 2016Co-Authors: Tomas Persson, Petar Pujic, Nicole Alloisio, Philippe Normand, Alison M. Berry, Thanh Van Nguyen, Katharina PawlowskiAbstract:To gain more insight in nitrogen metabolism in Actinorhizal nodules, a comparison between the N metabolite profiles in roots vs. nodules was initiated for one host plant from the best-examined order of Actinorhizal plants, Fagales, A. glutinosa (Betulaceae), a temperate tree, and one host plant from the Cucurbitales order, Datisca glomerata (Datiscaceae). For both symbioses, the symbiotic transcriptomes have been published and can be used to assess the expression of genes representing specific metabolic pathways in nodules. The amino acid profiles of roots in this study suggest that A. glutinosa transported aspartate, glutamate and citrulline in the xylem, a combination of nitrogenous solutes not published previously for this species. The amino acid profiles of D. glomerata roots depended on whether the plants were nodulated or grown on nitrate; roots of nodulated plants contained increased amounts of arginine. Although bacterial transcriptome data showed no symbiotic auxotrophy for branched chain amino acids (leucine, isoleucine, valine) in either symbiosis, D. glomerata nodules contained comparatively high levels of these amino acids. This might represent a response to osmotic stress.
Didier Bogusz - One of the best experts on this subject based on the ideXlab platform.
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Chitotetraose activates the fungal-dependent endosymbiotic signaling pathway in Actinorhizal plant species
PLoS ONE, 2019Co-Authors: Mireille Chabaud, Claudine Franche, Valérie Hocher, Leandro Imanishi, Joëlle Fournier, Lukas Brichet, Iltaf Abdou-pavy, Laurent Brottier, Elodie Pirolles, Didier BoguszAbstract:Mutualistic plant-microbe associations are widespread in natural ecosystems and have made major contributions throughout the evolutionary history of terrestrial plants. Amongst the most remarkable of these are the so-called root endosymbioses, resulting from the intra-cellular colonization of host tissues by either arbuscular mycorrhizal (AM) fungi or nitrogen-fixing bacteria that both provide key nutrients to the host in exchange for energy-rich photo-synthates. Actinorhizal host plants, members of the Eurosid 1 clade, are able to associate with both AM fungi and nitrogen-fixing actinomycetes known as Frankia. Currently, little is known about the molecular signaling that allows these plants to recognize their fungal and bacterial partners. In this article, we describe the use of an in vivo Ca 2+ reporter to identify symbiotic signaling responses to AM fungi in roots of both Casuarina glauca and Discaria tri-nervis, Actinorhizal species with contrasting modes of Frankia colonization. This approach has revealed that, for both Actinorhizal hosts, the short-chain chitin oligomer chitotetraose is able to mimic AM fungal exudates in activating the conserved symbiosis signaling pathway (CSSP) in epidermal root cells targeted by AM fungi. These results mirror findings in other AM host plants including legumes and the monocot rice. In addition, we show that chitote-traose is a more efficient elicitor of CSSP activation compared to AM fungal lipo-chitooligo-saccharides. These findings reinforce the likely role of short-chain chitin oligomers during the initial stages of the AM association, and are discussed in relation to both our current knowledge about molecular signaling during Frankia recognition as well as the different microsymbiont root colonization mechanisms employed by Actinorhizal hosts. PLOS ONE | https://doi.org/10.1371/journal.pone.
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chitinase resistant hydrophilic symbiotic factors secreted by frankia activate both ca2 spiking and nin gene expression in the Actinorhizal plant casuarina glauca
New Phytologist, 2016Co-Authors: Mireille Chabaud, Claudine Franche, Didier Bogusz, Hassen Gherbi, Virginie Vaissayre, Louis S Tisa, Joëlle Fournier, Elodie Pirolles, Daniel Moukouanga, David G BarkerAbstract:Although it is now well-established that decorated lipo-chitooligosaccharide Nod factors are the key rhizobial signals which initiate infection/nodulation in host legume species, the identity of the equivalent microbial signaling molecules in the Frankia/Actinorhizal association remains elusive. With the objective of identifying Frankia symbiotic factors we present a novel approach based on both molecular and cellular pre-infection reporters expressed in the model Actinorhizal species Casuarina glauca. By introducing the nuclear-localized cameleon Nup-YC2.1 into Casuarina glauca we show that cell-free culture supernatants of the compatible Frankia CcI3 strain are able to elicit sustained high frequency Ca(2+) spiking in host root hairs. Furthermore, an excellent correlation exists between the triggering of nuclear Ca(2+) spiking and the transcriptional activation of the ProCgNIN:GFP reporter as a function of the Frankia strain tested. These two pre-infection symbiotic responses have been used in combination to show that the signal molecules present in the Frankia CcI3 supernatant are hydrophilic, of low molecular weight and resistant to chitinase degradation. In conclusion, the biologically active symbiotic signals secreted by Frankia appear to be chemically distinct from the currently known chitin-based rhizobial/arbuscular mycorrhizal signaling molecules. Convenient bioassays in Casuarina glauca are now available for their full characterization.
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Plant and Soil 254: 229–237, 2003. © 2003 Kluwer Academic Publishers. Printed in the Netherlands.
2013Co-Authors: Carole Santi, Didier Bogusz, Florence Auguy, Emile Duhoux, Sergio Svistoonoff, Laure Constans, Claudine FrancheAbstract:Choosing a reporter for gene expression studies in transgenic Actinorhizal plants of the Casuarinaceae famil
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Silencing of the chalcone synthase gene in Casuarina glauca highlights the important role of flavonoids during nodulation
New Phytologist, 2013Co-Authors: Khalid Abdel-lateif, Claudine Franche, Hassen Gherbi, Sergio Svistoonoff, Virginie Vaissayre, Clotilde Verries, Emmanuelle Meudec, Francine Perrine-walker, Veronique Cheynier, Didier BoguszAbstract:Nitrogen-fixing root nodulation is confined to four plant orders, including > 14 000 Leguminosae, one nonlegume genus Parasponia and c. 200 Actinorhizal species that form symbioses with rhizobia and Frankia bacterial species, respectively. Flavonoids have been identified as plant signals and developmental regulators for nodulation in legumes and have long been hypothesized to play a critical role during Actinorhizal nodulation. However, direct evidence of their involvement in Actinorhizal symbiosis is lacking. Here, we used RNA interference to silence chalcone synthase, which is involved in the first committed step of the flavonoid biosynthetic pathway, in the Actinorhizal tropical tree Casuarina glauca. Transformed flavonoid-deficient hairy roots were generated and used to study flavonoid accumulation and further nodulation. Knockdown of chalcone synthase expression reduced the level of specific flavonoids and resulted in severely impaired nodulation. Nodule formation was rescued by supplementing the plants with naringenin, which is an upstream intermediate in flavonoid biosynthesis. Our results provide, for the first time, direct evidence of an important role for flavonoids during the early stages of Actinorhizal nodulation.
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the role of flavonoids in the establishment of plant roots endosymbioses with arbuscular mycorrhiza fungi rhizobia and frankia bacteria
Plant Signaling & Behavior, 2012Co-Authors: Khalid Abdellateif, Didier Bogusz, Valérie HocherAbstract:Flavonoids are a group of secondary metabolites derived from the phenylpropanoid pathway. They are ubiquitous in the plant kingdom and have many diverse functions including key roles at different levels of root endosymbioses. While there is a lot of information on the role of particular flavonoids in the Rhizobium-legume symbiosis, yet their exact role during the establishment of arbuscular mycorrhiza and Actinorhizal symbioses still remains unclear. Within the context of the latest data suggesting a common symbiotic signaling pathway for both plant-fungal and plant bacterial endosymbioses between legumes and Actinorhiza-forming fagales, this mini-review highlights some of the recent studies on the three major types of root endosymbioses. Implication of the molecular knowledge of endosymbioses signaling and genetic manipulation of flavonoid biosynthetic pathway on the development of strategies for the transfer and optimization of nodulation are also discussed.
Claudine Franche - One of the best experts on this subject based on the ideXlab platform.
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Chitotetraose activates the fungal-dependent endosymbiotic signaling pathway in Actinorhizal plant species
PLoS ONE, 2019Co-Authors: Mireille Chabaud, Claudine Franche, Valérie Hocher, Leandro Imanishi, Joëlle Fournier, Lukas Brichet, Iltaf Abdou-pavy, Laurent Brottier, Elodie Pirolles, Didier BoguszAbstract:Mutualistic plant-microbe associations are widespread in natural ecosystems and have made major contributions throughout the evolutionary history of terrestrial plants. Amongst the most remarkable of these are the so-called root endosymbioses, resulting from the intra-cellular colonization of host tissues by either arbuscular mycorrhizal (AM) fungi or nitrogen-fixing bacteria that both provide key nutrients to the host in exchange for energy-rich photo-synthates. Actinorhizal host plants, members of the Eurosid 1 clade, are able to associate with both AM fungi and nitrogen-fixing actinomycetes known as Frankia. Currently, little is known about the molecular signaling that allows these plants to recognize their fungal and bacterial partners. In this article, we describe the use of an in vivo Ca 2+ reporter to identify symbiotic signaling responses to AM fungi in roots of both Casuarina glauca and Discaria tri-nervis, Actinorhizal species with contrasting modes of Frankia colonization. This approach has revealed that, for both Actinorhizal hosts, the short-chain chitin oligomer chitotetraose is able to mimic AM fungal exudates in activating the conserved symbiosis signaling pathway (CSSP) in epidermal root cells targeted by AM fungi. These results mirror findings in other AM host plants including legumes and the monocot rice. In addition, we show that chitote-traose is a more efficient elicitor of CSSP activation compared to AM fungal lipo-chitooligo-saccharides. These findings reinforce the likely role of short-chain chitin oligomers during the initial stages of the AM association, and are discussed in relation to both our current knowledge about molecular signaling during Frankia recognition as well as the different microsymbiont root colonization mechanisms employed by Actinorhizal hosts. PLOS ONE | https://doi.org/10.1371/journal.pone.
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Review Article Use of Frankia and Actinorhizal Plants for Degraded Lands Reclamation
2016Co-Authors: Nathalie Diagne, Mariama Ngom, Claudine Franche, Mathish Nambiar-veetil, Karthikeyan Arumugam, Krishna Kumar Narayanan, Laurent LaplazeAbstract:Copyright © 2013 Nathalie Diagne et al.This is an open access article distributed under theCreativeCommonsAttribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. Degraded lands are defined by soils that have lost primary productivity due to abiotic or biotic stresses. Among the abiotic stresses, drought, salinity, and heavy metals are the main threats in tropical areas. These stresses affect plant growth and reduce their productivity. Nitrogen-fixing plants such as Actinorhizal species that are able to grow in poor and disturbed soils are widely planted for the reclamation of such degraded lands. It has been reported that association of soil microbes especially the nitrogen-fixing bacteria Frankia with these Actinorhizal plants can mitigate the adverse effects of abiotic and biotic stresses. Inoculation of Actinorhizal plants with Frankia significantly improves plant growth, biomass, shoot and root N content, and survival rate after transplanting in fields. However, the success of establishment of Actinorhizal plantation in degraded sites depends upon the choice of effective strains of Frankia. Studies related to the beneficial role of Frankia on the establishment of Actinorhizal plants i
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chitinase resistant hydrophilic symbiotic factors secreted by frankia activate both ca2 spiking and nin gene expression in the Actinorhizal plant casuarina glauca
New Phytologist, 2016Co-Authors: Mireille Chabaud, Claudine Franche, Didier Bogusz, Hassen Gherbi, Virginie Vaissayre, Louis S Tisa, Joëlle Fournier, Elodie Pirolles, Daniel Moukouanga, David G BarkerAbstract:Although it is now well-established that decorated lipo-chitooligosaccharide Nod factors are the key rhizobial signals which initiate infection/nodulation in host legume species, the identity of the equivalent microbial signaling molecules in the Frankia/Actinorhizal association remains elusive. With the objective of identifying Frankia symbiotic factors we present a novel approach based on both molecular and cellular pre-infection reporters expressed in the model Actinorhizal species Casuarina glauca. By introducing the nuclear-localized cameleon Nup-YC2.1 into Casuarina glauca we show that cell-free culture supernatants of the compatible Frankia CcI3 strain are able to elicit sustained high frequency Ca(2+) spiking in host root hairs. Furthermore, an excellent correlation exists between the triggering of nuclear Ca(2+) spiking and the transcriptional activation of the ProCgNIN:GFP reporter as a function of the Frankia strain tested. These two pre-infection symbiotic responses have been used in combination to show that the signal molecules present in the Frankia CcI3 supernatant are hydrophilic, of low molecular weight and resistant to chitinase degradation. In conclusion, the biologically active symbiotic signals secreted by Frankia appear to be chemically distinct from the currently known chitin-based rhizobial/arbuscular mycorrhizal signaling molecules. Convenient bioassays in Casuarina glauca are now available for their full characterization.
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Plant and Soil 254: 229–237, 2003. © 2003 Kluwer Academic Publishers. Printed in the Netherlands.
2013Co-Authors: Carole Santi, Didier Bogusz, Florence Auguy, Emile Duhoux, Sergio Svistoonoff, Laure Constans, Claudine FrancheAbstract:Choosing a reporter for gene expression studies in transgenic Actinorhizal plants of the Casuarinaceae famil
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Silencing of the chalcone synthase gene in Casuarina glauca highlights the important role of flavonoids during nodulation
New Phytologist, 2013Co-Authors: Khalid Abdel-lateif, Claudine Franche, Hassen Gherbi, Sergio Svistoonoff, Virginie Vaissayre, Clotilde Verries, Emmanuelle Meudec, Francine Perrine-walker, Veronique Cheynier, Didier BoguszAbstract:Nitrogen-fixing root nodulation is confined to four plant orders, including > 14 000 Leguminosae, one nonlegume genus Parasponia and c. 200 Actinorhizal species that form symbioses with rhizobia and Frankia bacterial species, respectively. Flavonoids have been identified as plant signals and developmental regulators for nodulation in legumes and have long been hypothesized to play a critical role during Actinorhizal nodulation. However, direct evidence of their involvement in Actinorhizal symbiosis is lacking. Here, we used RNA interference to silence chalcone synthase, which is involved in the first committed step of the flavonoid biosynthetic pathway, in the Actinorhizal tropical tree Casuarina glauca. Transformed flavonoid-deficient hairy roots were generated and used to study flavonoid accumulation and further nodulation. Knockdown of chalcone synthase expression reduced the level of specific flavonoids and resulted in severely impaired nodulation. Nodule formation was rescued by supplementing the plants with naringenin, which is an upstream intermediate in flavonoid biosynthesis. Our results provide, for the first time, direct evidence of an important role for flavonoids during the early stages of Actinorhizal nodulation.
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The plant-growth-promoting actinobacteria of the genus Nocardia induces root nodule formation in Casuarina glauca
Antonie van Leeuwenhoek, 2019Co-Authors: Faten Ghodhbane-gtari, Laurent Laplaze, Hassen Gherbi, Imen Nouioui, Virginie Vaissayre, Karima Hezbri, Emily Lundstedt, Timothy D’angelo, Zakkary Mcnutt, Sergio SvistoonoffAbstract:Actinorhizal plants form a symbiotic association with the nitrogen-fixing actinobacteria Frankia. These plants have important economic and ecological benefits including land reclamation, soil stabilization, and reforestation. Recently, many non-Frankia actinobacteria have been isolated from Actinorhizal root nodules suggesting that they might contribute to nodulation. Two Nocardia strains, BMG51109 and BMG111209, were isolated from Casuarina glauca nodules, and they induced root nodule-like structures in original host plant promoting seedling growth. The formed root nodule-like structures lacked a nodular root at the apex, were not capable of reducing nitrogen and had their cortical cells occupied with rod-shaped Nocardiae cells. Both Nocardia strains induced root hair deformation on the host plant. BMG111209 strain induced the expression of the ProCgNin:Gus gene, a plant gene involved in the early steps of the infection process and nodulation development. Nocardia strain BMG51109 produced three types of auxins (Indole-3-acetic acid [IAA], Indole-3-Byturic Acid [IBA] and Phenyl Acetic Acid [PAA]), while Nocardia BMG111209 only produced IAA. Analysis of the Nocardia genomes identified several important predicted biosynthetic gene clusters for plant phytohormones, secondary metabolites, and novel natural products. Co-infection studies showed that Nocardia strain BMG51109 plays a role as a helper bacteria promoting an earlier onset of nodulation. This study raises many questions on the ecological significance and functionality of Nocardia bacteria in Actinorhizal symbioses.
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Review Article Use of Frankia and Actinorhizal Plants for Degraded Lands Reclamation
2016Co-Authors: Nathalie Diagne, Mariama Ngom, Claudine Franche, Mathish Nambiar-veetil, Karthikeyan Arumugam, Krishna Kumar Narayanan, Laurent LaplazeAbstract:Copyright © 2013 Nathalie Diagne et al.This is an open access article distributed under theCreativeCommonsAttribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. Degraded lands are defined by soils that have lost primary productivity due to abiotic or biotic stresses. Among the abiotic stresses, drought, salinity, and heavy metals are the main threats in tropical areas. These stresses affect plant growth and reduce their productivity. Nitrogen-fixing plants such as Actinorhizal species that are able to grow in poor and disturbed soils are widely planted for the reclamation of such degraded lands. It has been reported that association of soil microbes especially the nitrogen-fixing bacteria Frankia with these Actinorhizal plants can mitigate the adverse effects of abiotic and biotic stresses. Inoculation of Actinorhizal plants with Frankia significantly improves plant growth, biomass, shoot and root N content, and survival rate after transplanting in fields. However, the success of establishment of Actinorhizal plantation in degraded sites depends upon the choice of effective strains of Frankia. Studies related to the beneficial role of Frankia on the establishment of Actinorhizal plants i
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Tolerance to environmental stress by the nitrogen-fixing actinobacterium Frankia and its role in Actinorhizal plants adaptation
Symbiosis, 2016Co-Authors: Mariama Ngom, Laurent Laplaze, Sergio Svistoonoff, Maimouna Cissoko, Louis S Tisa, Nathalie Diagne, Rediet Oshone, Antony ChampionAbstract:Environmental stresses are caused by human activities or natural events. Several of them including salinity, heavy metals, and extreme temperature affect both soil characteristics and plant growth and productivity. Actinorhizal plants are pioneer species that are able to grow in poor soils and improve soil fertility. They are widely used in agroforestry for different purposes including reclamation of degraded and contaminated lands. This capacity is mainly due to the plants forming a nitrogen-fixing symbiosis with actinobacteria known as Frankia . In comparison to uninoculated plants, plants in symbiosis with Frankia have significantly improved plant growth, total biomass, and nitrogen and chlorophyll content which enhance the development of Actinorhizal plants and their resistance to abiotic stresses. However, to optimize the adaptation of Actinorhizal species to different environments, selection of both symbiotic partners is necessary. Frankia strains vary in their sensitivity and response to stress including salinity, heavy metals, extreme pH and drought. In this paper, we review the response of different Frankia strains to environmental stresses and their role that they play in the adaptation of Actinorhizal plants to stressful conditions.
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Role of auxin during intercellular infection of Discaria trinervis by Frankia
Frontiers in Plant Science, 2014Co-Authors: Leandro Imanishi, Antony Champion, Laurent Laplaze, Francine Perrine-walker, Adama Ndour, Alice Vayssieres, Geneviève Conejero, Mikael Lucas, Luis Wall, Sergio SvistoonoffAbstract:ogen-fixing nodules induced by Frankia in the Actinorhizal plant Discaria trinervis result from a primitive intercellular root invasion pathway that does not involve root hair deformation and infection threads. Here, we analyzed the role of auxin in this intercellular infection pathway at the molecular level and compared it with our previous work in the intracellular infected Actinorhizal plant Casuarina glauca. Immunolocalisation experiments showed that auxin accumulated in Frankia-infected cells in both systems. We then characterized the expression of auxin transporters in D. trinervis nodules. No activation of the heterologous CgAUX1 promoter was detected in infected cells in D. trinervis. These results were confirmed with the endogenous D. trinervis gene, DtAUX1. However, DtAUX1 was expressed in the nodule meristem. Consistently, transgenic D. trinervis plants containing the auxin response marker DR5:VENUS showed expression of the reporter gene in the meristem. Immunolocalisation experiments using an antibody against the auxin efflux carrier PIN1, revealed the presence of this transporter in the plasma membrane of infected cells. Finally, we used in silico cellular models to analyse auxin fluxes in D. trinervis nodules. Our results point to the existence of divergent roles of auxin in intercellularly- and intracellularly-infected Actinorhizal plants, an ancestral infection pathways leading to root nodule symbioses.
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casuarina root exudates alter the physiology surface properties and plant infectivity of frankia sp strain cci3
Applied and Environmental Microbiology, 2012Co-Authors: Nicholas Beauchemin, Didier Bogusz, Laurent Laplaze, Sergio Svistoonoff, Patrick Doumas, Teal Furnholm, Julien Lavenus, Louis S TisaAbstract:ABSTRACT The actinomycete genus Frankia forms nitrogen-fixing symbioses with 8 different families of Actinorhizal plants, representing more than 200 different species. Very little is known about the initial molecular interactions between Frankia and host plants in the rhizosphere. Root exudates are important in Rhizobium-legume symbiosis, especially for initiating Nod factor synthesis. We measured differences in Frankia physiology after exposure to host aqueous root exudates to assess their effects on Actinorhizal symbioses. Casuarina cunninghamiana root exudates were collected from plants under nitrogen-sufficient and -deficient conditions and tested on Frankia sp. strain CcI3. Root exudates increased the growth yield of Frankia in the presence of a carbon source, but Frankia was unable to use the root exudates as a sole carbon or energy source. Exposure to root exudates caused hyphal “curling” in Frankia cells, suggesting a chemotrophic response or surface property change. Exposure to root exudates altered Congo red dye binding, which indicated changes in the bacterial surface properties at the fatty acid level. Fourier transform infrared spectroscopy (FTIR) confirmed fatty acid changes and revealed further carbohydrate changes. Frankia cells preexposed to C. cunninghamiana root exudates for 6 days formed nodules on the host plant significantly earlier than control cells. These data support the hypothesis of early chemical signaling between Actinorhizal host plants and Frankia in the rhizosphere.
