The Experts below are selected from a list of 231 Experts worldwide ranked by ideXlab platform
Claus Koch - One of the best experts on this subject based on the ideXlab platform.
-
Immunopurified extracellular Bartonella henselae antigen for detecting specific antibodies by enzyme immunoassay.
Apmis, 1997Co-Authors: Kraesten Engbaek, Lars Otto Uttenthal, Claus KochAbstract:Protein antigens of Bartonella henselae bacterial sonicate supernatant and concentrated cell-free culture filtrate were examined by SDS-PAGE. The sonicate supernatant gave 38 bands and the culture filtrate at least 21, of which 18 were of bacterial origin. Immunoblotting against 13 monoclonal antibodies obtained from mice infected with live B. henselae showed that 10 of these antibodies reacted with a narrow 225 kDa band and varying smears of bands ranging from 36 to 240 kDa in the sonicate, but only with a single 200 kDa band in the culture filtrate. Testing of pre- and post-infection rabbit sera in immunoblotting against culture filtrate demonstrated that the 200 kDa component gave the most prominent specific reaction with post-infection sera. The 200 kDa antigen was isolated by immunoaffinity chromatography of concentrated culture filtrate, and its molecular size determined by size-exclusion chromatography as >1000 kDa. The immunopurified antigen was compared with bacterial sonicate as coating antigen in EIA for determining humoral immune responses in rabbits inoculated with live B. henselae. The two antigens gave almost identical results for IgM and IgG responses. The specificity of the immunopurified antigen was tested in EIA against hyperimmune rabbit sera and sera of rabbits inoculated with live B. henselae, B. quintana and Afipia Felis. Only the hyperimmune serum against B. henselae and the sera of the rabbits inoculated with live B. henselae reacted with the immunopurified antigen, whereas the B. henselae sonicate cross-reacted with hyperimmune and post-infection sera of rabbits inoculated with B. quintana and A. Felis.
-
production and characterization of mouse monoclonal antibodies against Afipia Felis
Apmis, 1997Co-Authors: Kraesten Engbaek, Lars Otto Uttenthal, Claus KochAbstract:: A series of 10 monoclonal antibodies reacting with Afipia Felis antigens were selected from mice immunized with live organisms of the reference strain ATCC 53690. Immunoblotting against SDS-PAGE-separated A Felis sonicate allowed the antibodies to be classified into three groups: 1) 168-4, -6, -7 and -10 reacted with a 53 kDa antigen, 2) 168-1, -3 and -9 reacted with both 53 kDa and 60 kDa antigens, and 3) 168-2, -5 and -9 reacted with other antigens. Antibodies of group 1 did not cross-react with other Afipia species or 36 unrelated bacteria, whereas those of groups 2 and 3 reacted with other Afipia species and some unrelated bacteria. Immunoblots of crossed immunoelectrophoretic patterns of A. Felis sonicate against rabbit antiserum showed that antibodies of groups 1 and 2 bound to the same precipitin arcs. Antibodies of group 1 reacted with a species-specific epitope on the 53 kDa antigen, while those of group 2 reacted with other epitopes shared by the 53 kDa and 60 kDa antigens. The binding of antibodies of group 1 to A. Felis sonicate was inhibited by post-infection rabbit serum, whereas no inhibition was observed for antibodies of group 2. The species-specific epitope of the 53 kDa antigen and the early appearance of antibodies against this epitope after infection suggest that this antigen can be used in a serodiagnostic test for A. Felis infection.
-
identification of Afipia Felis antigens in culture medium reaction with human sera
Apmis, 1997Co-Authors: Kraesten Engbaek, Lars Otto Uttenthal, Claus KochAbstract:: Fourteen protein antigens were identified on SDS-PAGE of Afipia Felis culture supernatant. Immunoblotting against 10 monoclonal antibodies obtained from mice infected with live A. Felis showed that 4 antibodies reacted with a 56 kDa band and 3 with both 56 kDa and 62 kDa bands. Compared with A. Felis sonicate, the reacting proteins in culture supernatant showed an increase in molecular mass of 2-3 kDa, suggesting that they were more glycosylated. Purified antigen obtained by affinity chromatography of culture supernatant on the seven immobilized antibodies was tested against antibodies reacting with the 56 kDa and 62 kDa bands. All eluates contained both components, suggesting that the antibodies were directed against different epitopes of a double antigen held together during the affinity chromatography but cleaved by reduction and SDS-PAGE. The molecular size of the uncleaved protein in culture supernatant was determined by size-exclusion chromatography as > 1000 kDa. Testing of pre- and post-infection rabbit sera in immunoblotting against culture supernatant demonstrated that the 56 kDa and 62 kDa components gave the most prominent specific reactions with post-infection sera. One of fifty human sera submitted for testing for cat-scratch disease and 1 of 50 sera from healthy blood donors reacted with several bands in A. Felis culture supernatant, including the 56 kDa and 62 kDa bands.
-
antibody response in rabbits infected with rochalimaea henselae rochalimaea quintana and Afipia Felis
Apmis, 1994Co-Authors: Kraesten Engbaek, Claus KochAbstract:: Antibody responses in three pairs of rabbits inoculated with live Rochalimaea henselae, Rochalimaea quintana and Afipia Felis were studied by enzyme immunoassay with whole-cell and bacterial sonicates as antigen. No differences in measured antibody responses were found with the two types of antigen preparation. Two rabbits did not respond with antibody production. In the remaining rabbits there was a low-titred antibody response that showed no significant cross-reaction with related bacteria. After rechallenge the antibody response rose significantly and there was significant cross-reaction with related bacteria. The antigens involved in the antibody response were examined by crossed immunoelectrophoresis. After the initial inoculation 5-7 precipitin lines of the reference diagrams were deflected, including lines which cross-reacted with antigens found in related bacterial species. After reinoculation several more precipitin lines were deflected, including additional lines cross-reacting with antigens present in related bacteria and common bacterial antigens.
-
immunoelectrophoretic characterization and cross reactivity of rochalimaea henselae rochalimaea quintana and Afipia Felis
Apmis, 1994Co-Authors: Kraesten Engbaek, Claus KochAbstract:: The soluble antigens of Rochalimaea henselae, Rochalimaea quintana and Afipia Felis were characterized by crossed immunoelectrophoresis using bacterial sonicates as antigens against pooled hyperimmune rabbit sera. A precipitin pattern was drawn for each bacterium and shown to be reproducible and stable even when normal or preimmune rabbit serum was incorporated in the intermediate gel. By this technique 56 antigens were identified from R. henselae, 49 from R. quintana, and 39 from A. Felis. The serological cross-reaction between R. henselae, R. quintana and A. Felis, and between these 3 bacteria and 32 pathogenic bacteria was analysed by rocket-line immunoelectrophoresis, crossed-line immunoelectrophoresis, and tandem-crossed electrophoresis. It was concluded that (i) 4-7 antigens distinguish R. henselae, R. quintana and A. Felis from each other, (ii) both Gram-positive and Gram-negative bacteria cross-react with R. henselae, R. quintana and A. Felis antisera, (iii) the cross-reacting antigens of Gram-negative bacteria have both precipitating and non-precipitating specificities, whereas Gram-positive bacteria have mainly non-precipitating specificities, (iv) the cross-reacting antigens are common to several species, and (v) fewer cross-reacting antigens are found in phylogenetically disparate species than in more closely related species.
Didier Raoult - One of the best experts on this subject based on the ideXlab platform.
-
description of Afipia birgiae sp nov and Afipia massiliensis sp nov and recognition of Afipia Felis genospecies a
International Journal of Systematic and Evolutionary Microbiology, 2002Co-Authors: Bernard La Scola, Marienoelle Mallet, Patrick A D Grimont, Didier RaoultAbstract:On the basis of phenotypic characterization and DNA relatedness, two novel species are proposed, Afipia birgiae sp. nov. (type strain 34632T = CIP 106344T = CCUG 43108T) and Afipia massiliensis sp. nov. (type strain 34633T = CIP 107022T = CCUG 45153T). A new genospecies is described, named Afipia Felis genospecies A, closely related to Afipia Felis. The complexity encountered in the taxonomy of the Bradyrhizobiaceae group within the alpha-2 subgroup of the Proteobacteria is discussed and the description of these novel species highlights the need for new tools for phylogenetic analysis in the group. The novel species herein described are fastidious bacteria isolated from a hospital water supply in co-culture with amoebae. It is hypothesized that this group of bacteria are a potential cause of nosocomial infections.
-
Legionella-like and other amoebal pathogens as agents of community-acquired pneumonia.
Emerging Infectious Diseases, 2001Co-Authors: Thomas J. Marrie, Richard J. Birtles, Bernard La Scola, Didier Raoult, Elena De CarolisAbstract:We tested serum specimens from three groups of patients with pneumonia by indirect immunofluorescence against Legionella-like amoebal pathogens (LLAPs) 1–7, 9, 10, 12, 13; Parachlamydia acanthamoeba strains BN 9 and Hall’s coccus; and Afipia Felis. We found that LLAPs play a role (albeit an infrequent one) in community-acquired pneumonia, usually as a co-pathogen but sometimes as the sole identified pathogen. A number of bacteria that grow only within amoebae and are closely related phylogenetically to Legionella species, Legionella -like amoebal pathogens (LLAPs), have been identified and characterized (1). The role of these bacteria as human pathogens is still largely unknown. Other microorganisms, e.g., Parachlamydia acanthamoeba strains BN 9 (2) and Hall’s coccus (3), also grow within amoebae. Afipia Felis (once thought to be the etiologic agent of cat-scratch disease), a gram-negative rod, is difficult to grow on artificial medium but grows well in human monocytes and HeLa cells (4); this organism was recently reported to be an environmental bacterium probably associated with free-living amoebae and living in water (5). We tested serum specimens from three groups of patients with pneumonia to determine if any of these microorganisms cause disease. The Study We used 511 specimens from a 1985 study of a random sample of the Nova Scotia population (6); 121 acute- and convalescent-phase serum specimens from a study (Nova Scotia, 1991-1994) of 149 ambulatory patients with communityacquired pneumonia (7); and specimens from a prospective study of community-acquired pneumonia requiring hospitalization conducted at 15 teaching hospitals in eight Canadian provinces (1996-1997). All serum specimens from both groups of patients with pneumonia were tested for antibodies to Mycoplasma pneumoniae; influenza viruses A and B; parainfluenza viruses 1,2,3; adenovirus; and Respiratory syncytial virus (RSV) by a standard complement fixation technique in microtiter plates. Serum specimens from 60% of the patients (randomly selected from the group of patients with community-acquired pneumonia requiring hospitalization) were tested by the microimmunofluorescence test (8-10) for immunoglobulin (Ig) G and IgM antibodies to Chlamydia pneumoniae (AR 39 strain); C. psittaci (avian strain 6BC, feline pneumonitis strain FP, turkey strain TT 3, and pigeon strain CP 3); C. pecorum (ovine polyarthritis strain); and C. trachomatis
-
isolation of new fastidious α proteobacteria and Afipia Felis from hospital water supplies by direct plating and amoebal co culture procedures
FEMS Microbiology Ecology, 2000Co-Authors: Bernard La Scola, Lina Barrassi, Didier RaoultAbstract:As water is a source of nosocomial infections in hospitals, the presence of fastidious Gram-negative bacteria in water samples taken in a university hospital was investigated. Water samples were inoculated onto agar plates and into amoebal microplates for co-culture. Sixty-eight α proteobacteria isolates were obtained and characterized using phenotypic methods and 16S rRNA gene sequence comparison. The latter approach divided the strains into seven clusters. Of these, one corresponded to previously recognized Afipia Felis and it is likely that six were closely related new species. As these bacteria are fastidious and can not be cultivated on standard microbiological media, their possible role in hospital-acquired human infections should be investigated.
-
evaluation of serological response to bartonella henselae bartonella quintana and Afipia Felis antigens in 64 patients with suspected cat scratch disease
Scandinavian Journal of Infectious Diseases, 1996Co-Authors: M Dupon, Didier Raoult, A Savin M De Larclause, Philippe Brouqui, M Drancourt, A De Mascarel, J Y LacutAbstract:The serological response to Bartonella henselae, B. quintana, and Afipia Felis was assessed by an indirect fluorescence antibody test (IFAT) in 64 patients with suspected cat-scratch disease (CSD) recruited from the Bordeaux area in France. Blood samples were collected from 57 patients with chronic lymphadenopathy who underwent lymph-node biopsy with suggestive histopathologic features of CSD, and from an additional 7 patients with suspected CSD who underwent surgical incision and drainage because of lymph-node tenderness. Of the patients, 31 were male and 33 female, with a median age of 27 years (range 2–89). 69.8% reported cat and/or dog contact. Of the 26/64 (40.6%) patients, serum samples were positive at a titer of 1 : 100 or more for immunoglobulin G (IgG) antibodies (17 only to B. henselae, 1 only to B. quintana, 3 only to Afipia Felis, and 5 to both B. henselae and B. quintana). IgM or IgA antibodies were also detected in 10 patients with IgG antibodies to B. henselae. 11 (17.2%) of the 64 patient...
-
evaluation of serological response to bartonella henselae bartonella quintana and Afipia Felis antigens in 64 patients with suspected cat scratch disease
Interscience Conference on Antimicrobial Agents and Chemotherapy, 1996Co-Authors: M Dupon, Didier Raoult, A Savin M De Larclause, Philippe Brouqui, M Drancourt, A De Mascarel, J Y LacutAbstract:The serological response to Bartonella henselae, B. quintana, and Afipia Felis was assessed by an indirect fluorescence antibody test (IFAT) in 64 patients with suspected cat-scratch disease (CSD) recruited from the Bordeaux area in France. Blood samples were collected from 57 patients with chronic lymphadenopathy who underwent lymph-node biopsy with suggestive histopathologic features of CSD, and from an additional 7 patients with suspected CSD who underwent surgical incision and drainage because of lymph-node tenderness. Of the patients, 31 were male and 33 female, with a median age of 27 years (range 2-89). 69.8% reported cat and/or dog contact. Of the 26/64 (40.6%) patients, serum samples were positive at a titer of 1:100 or more for immunoglobulin G (IgG) antibodies (17 only to B. henselae, 1 only to B. quintana, 3 only to Afipia Felis, and 5 to both B. henselae and B. quintana). IgM or IgA antibodies were also detected in 10 patients with IgG antibodies to B. henselae. 11 (17.2%) of the 64 patient serum samples were positive at a low titer of 1:50. These data suggested that serological response assessed by standard IFAT is not enough to confirm a CSD diagnosis.
Kraesten Engbaek - One of the best experts on this subject based on the ideXlab platform.
-
Immunopurified extracellular Bartonella henselae antigen for detecting specific antibodies by enzyme immunoassay.
Apmis, 1997Co-Authors: Kraesten Engbaek, Lars Otto Uttenthal, Claus KochAbstract:Protein antigens of Bartonella henselae bacterial sonicate supernatant and concentrated cell-free culture filtrate were examined by SDS-PAGE. The sonicate supernatant gave 38 bands and the culture filtrate at least 21, of which 18 were of bacterial origin. Immunoblotting against 13 monoclonal antibodies obtained from mice infected with live B. henselae showed that 10 of these antibodies reacted with a narrow 225 kDa band and varying smears of bands ranging from 36 to 240 kDa in the sonicate, but only with a single 200 kDa band in the culture filtrate. Testing of pre- and post-infection rabbit sera in immunoblotting against culture filtrate demonstrated that the 200 kDa component gave the most prominent specific reaction with post-infection sera. The 200 kDa antigen was isolated by immunoaffinity chromatography of concentrated culture filtrate, and its molecular size determined by size-exclusion chromatography as >1000 kDa. The immunopurified antigen was compared with bacterial sonicate as coating antigen in EIA for determining humoral immune responses in rabbits inoculated with live B. henselae. The two antigens gave almost identical results for IgM and IgG responses. The specificity of the immunopurified antigen was tested in EIA against hyperimmune rabbit sera and sera of rabbits inoculated with live B. henselae, B. quintana and Afipia Felis. Only the hyperimmune serum against B. henselae and the sera of the rabbits inoculated with live B. henselae reacted with the immunopurified antigen, whereas the B. henselae sonicate cross-reacted with hyperimmune and post-infection sera of rabbits inoculated with B. quintana and A. Felis.
-
production and characterization of mouse monoclonal antibodies against Afipia Felis
Apmis, 1997Co-Authors: Kraesten Engbaek, Lars Otto Uttenthal, Claus KochAbstract:: A series of 10 monoclonal antibodies reacting with Afipia Felis antigens were selected from mice immunized with live organisms of the reference strain ATCC 53690. Immunoblotting against SDS-PAGE-separated A Felis sonicate allowed the antibodies to be classified into three groups: 1) 168-4, -6, -7 and -10 reacted with a 53 kDa antigen, 2) 168-1, -3 and -9 reacted with both 53 kDa and 60 kDa antigens, and 3) 168-2, -5 and -9 reacted with other antigens. Antibodies of group 1 did not cross-react with other Afipia species or 36 unrelated bacteria, whereas those of groups 2 and 3 reacted with other Afipia species and some unrelated bacteria. Immunoblots of crossed immunoelectrophoretic patterns of A. Felis sonicate against rabbit antiserum showed that antibodies of groups 1 and 2 bound to the same precipitin arcs. Antibodies of group 1 reacted with a species-specific epitope on the 53 kDa antigen, while those of group 2 reacted with other epitopes shared by the 53 kDa and 60 kDa antigens. The binding of antibodies of group 1 to A. Felis sonicate was inhibited by post-infection rabbit serum, whereas no inhibition was observed for antibodies of group 2. The species-specific epitope of the 53 kDa antigen and the early appearance of antibodies against this epitope after infection suggest that this antigen can be used in a serodiagnostic test for A. Felis infection.
-
identification of Afipia Felis antigens in culture medium reaction with human sera
Apmis, 1997Co-Authors: Kraesten Engbaek, Lars Otto Uttenthal, Claus KochAbstract:: Fourteen protein antigens were identified on SDS-PAGE of Afipia Felis culture supernatant. Immunoblotting against 10 monoclonal antibodies obtained from mice infected with live A. Felis showed that 4 antibodies reacted with a 56 kDa band and 3 with both 56 kDa and 62 kDa bands. Compared with A. Felis sonicate, the reacting proteins in culture supernatant showed an increase in molecular mass of 2-3 kDa, suggesting that they were more glycosylated. Purified antigen obtained by affinity chromatography of culture supernatant on the seven immobilized antibodies was tested against antibodies reacting with the 56 kDa and 62 kDa bands. All eluates contained both components, suggesting that the antibodies were directed against different epitopes of a double antigen held together during the affinity chromatography but cleaved by reduction and SDS-PAGE. The molecular size of the uncleaved protein in culture supernatant was determined by size-exclusion chromatography as > 1000 kDa. Testing of pre- and post-infection rabbit sera in immunoblotting against culture supernatant demonstrated that the 56 kDa and 62 kDa components gave the most prominent specific reactions with post-infection sera. One of fifty human sera submitted for testing for cat-scratch disease and 1 of 50 sera from healthy blood donors reacted with several bands in A. Felis culture supernatant, including the 56 kDa and 62 kDa bands.
-
antibody response in rabbits infected with rochalimaea henselae rochalimaea quintana and Afipia Felis
Apmis, 1994Co-Authors: Kraesten Engbaek, Claus KochAbstract:: Antibody responses in three pairs of rabbits inoculated with live Rochalimaea henselae, Rochalimaea quintana and Afipia Felis were studied by enzyme immunoassay with whole-cell and bacterial sonicates as antigen. No differences in measured antibody responses were found with the two types of antigen preparation. Two rabbits did not respond with antibody production. In the remaining rabbits there was a low-titred antibody response that showed no significant cross-reaction with related bacteria. After rechallenge the antibody response rose significantly and there was significant cross-reaction with related bacteria. The antigens involved in the antibody response were examined by crossed immunoelectrophoresis. After the initial inoculation 5-7 precipitin lines of the reference diagrams were deflected, including lines which cross-reacted with antigens found in related bacterial species. After reinoculation several more precipitin lines were deflected, including additional lines cross-reacting with antigens present in related bacteria and common bacterial antigens.
-
immunoelectrophoretic characterization and cross reactivity of rochalimaea henselae rochalimaea quintana and Afipia Felis
Apmis, 1994Co-Authors: Kraesten Engbaek, Claus KochAbstract:: The soluble antigens of Rochalimaea henselae, Rochalimaea quintana and Afipia Felis were characterized by crossed immunoelectrophoresis using bacterial sonicates as antigens against pooled hyperimmune rabbit sera. A precipitin pattern was drawn for each bacterium and shown to be reproducible and stable even when normal or preimmune rabbit serum was incorporated in the intermediate gel. By this technique 56 antigens were identified from R. henselae, 49 from R. quintana, and 39 from A. Felis. The serological cross-reaction between R. henselae, R. quintana and A. Felis, and between these 3 bacteria and 32 pathogenic bacteria was analysed by rocket-line immunoelectrophoresis, crossed-line immunoelectrophoresis, and tandem-crossed electrophoresis. It was concluded that (i) 4-7 antigens distinguish R. henselae, R. quintana and A. Felis from each other, (ii) both Gram-positive and Gram-negative bacteria cross-react with R. henselae, R. quintana and A. Felis antisera, (iii) the cross-reacting antigens of Gram-negative bacteria have both precipitating and non-precipitating specificities, whereas Gram-positive bacteria have mainly non-precipitating specificities, (iv) the cross-reacting antigens are common to several species, and (v) fewer cross-reacting antigens are found in phylogenetically disparate species than in more closely related species.
D. J. Brenner - One of the best experts on this subject based on the ideXlab platform.
-
cat scratch disease the rare role of Afipia Felis
Journal of Clinical Microbiology, 1998Co-Authors: Michael Giladi, D. J. Brenner, Boaz Avidor, Yehudith Kletter, Suzy Abulafia, Leonard N Slater, David F Welch, Arnold G Steigerwalt, Anne M Whitney, Moshe EphrosAbstract:Since its isolation in 1988, Afipia Felis has been associated with cat scratch disease (CSD) in only one report and its role in CSD has been questioned. We have cultured A. Felis from a lymph node of a patient with CSD. 16S rRNA gene sequencing, DNA relatedness studies, fatty acid analysis, and PCR of the A. Felis ferredoxin gene showed that the isolate is identical to the previously reported A. Felis isolate. To determine the role of A. Felis in CSD, PCR of the 16S rRNA gene followed by hybridizations with specific probes were performed with lymph node specimens from CSD patients. All 32 specimens tested positive for Bartonella henselae and negative for A. Felis. We conclude that A. Felis is a rare cause of CSD. Diagnostic tests not conducive to the identification of A. Felis might cause the diagnosis of CSD due to A. Felis to be missed.
-
Rochalimaea henselae sp. nov., a cause of septicemia, bacillary angiomatosis, and parenchymal bacillary peliosis.
Journal of Clinical Microbiology, 1992Co-Authors: David F Welch, Leonard N Slater, Arnold G Steigerwalt, D A Pickett, D. J. BrennerAbstract:Nine strains of Rochalimaea spp. that were isolated from patients over a period of 4.5 years were characterized for their enzyme activities, cellular fatty acid compositions, and DNA interrelatedness among Rochalimaea spp., Bartonella bacilliformis, and Afipia Felis (cat scratch disease bacillus). All except one isolate, which was Rochalimaea quintana, were determined to belong to a newly proposed species, Rochalimaea henselae sp. nov. After recovery from clinical material, colonies required 5 to 15 days of incubation to become apparent. Cells were small, gram-negative, curved bacilli and displayed twitching motility. Enzyme specificities for amino acid and carbohydrate substrates showed that R. henselae could be distinguished from Rochalimaea vinsonii by L-arginyl-L-arginine and L-lysyl-L-alanine peptidases, but not all strains could be distinguished from R. quintana on the basis of peptidases or carbohydrate utilization. R. henselae also closely resembled R. quintana in cellular fatty acid composition, with both consisting mainly of C18:1, C18:0, and C16:0 fatty acids. However, the strains of R. henselae all contained C18:0 in amounts averaging greater than or equal to 22%, in contrast to R. quintana, which contained this cellular fatty acid in amounts averaging 16 and 18%. DNA hybridization confirmed the identification of one clinical isolate as R. quintana and showed a close interrelatedness (92 to 100%) among the other strains. Under optimal conditions for DNA reassociation, R. henselae showed approximately 70% relatedness to R. quintana and approximately 60% relatedness to R. vinsonii. Relatedness with DNA from B. baciliformis was 43%. R. henselae was unrelated to A. Felis. R. henselae is the proposed species of a newly recognized member of the family Rickettsiaceae, which is a pathogen that may be encountered in immunocompromised or immunocompetent patients. Prolonged fever with bacteremia or vascular proliferative lesions are clinical manifestations of the agent.
-
proposal of Afipia gen nov with Afipia Felis sp nov formerly the cat scratch disease bacillus Afipia clevelandensis sp nov formerly the cleveland clinic foundation strain Afipia broomeae sp nov and three unnamed genospecies
Journal of Clinical Microbiology, 1991Co-Authors: D. J. Brenner, C. K. English, G S Hall, D G Hollis, William F. Bibb, J Vincent, C W Moss, Kristin A Birkness, J Radosevic, F. D. QuinnAbstract:On the basis of phenotypic characterization and DNA relatedness determinations, the genus Afipia gen. nov., which contains six species, is described. The type species is Afipia Felis sp. nov. (the cat scratch disease bacillus). Afipia clevelandensis sp. nov., Afipia broomeae sp. nov., and three unnamed not associated with cat-borne disease. All but one strain (Afipia genospecies 3) were isolated from human wound and respiratory sources. All Afipia species are gram-negative, oxidase-positive, nonfermentative rods in the alpha-2 subgroup of the class Proteobacteria. They are motile by means of a single flagellum. They grow on buffered charcoal-yeast extract agar and nutrient broth, but rarely on MacConkey agar, at 25 and 30 degrees C. They are urease positive; but they are negative in reactions for hemolysis, indole production, H2S production (triple sugar iron agar), gelatin hydrolysis, esculin hydrolysis, and peptonization of litmus milk. They do not produce acid oxidatively from D-glucose, lactose, maltose, or sucrose. The major cell wall fatty acids are 11-methyloctadec-12-enoic (CBr19:1), cis-octadec-11-enoic (C18:1omega7c), and generally, 9,10-methylenehexadecanote and 11,12-methyleneoctadecanoate; and there are only trace amounts of hydroxy acids. The guanineplus-cytosine content is 61.5 to 69 mol%. A. Felis is positive for nitrate reduction and is delayed positive for acid production from D-xylose, but it is catalase negative. A. clevelandensis is negative in all of these tests. A. broomeae is weakly positive for catalase production and acid production from D-xylose, but it is negative for nitrate reduction. Images
-
Proposal of Afipia gen. nov., with Afipia Felis sp. nov. (Formerly the cat scratch disease bacillus), Afipia clevelandensis sp. nov. (Formerly the Cleveland Clinic Foundation Strain), Afipia broomeae sp. nov., and three unnamed genospecies
Journal of Clinical Microbiology, 1991Co-Authors: D. J. Brenner, C. K. English, K. A. Birkness, G S Hall, D G Hollis, William F. Bibb, J Vincent, C W Moss, J. Radošević, F. D. QuinnAbstract:On the basis of phenotypic characterization and DNA relatedness determinations, the genus Afipia gen. nov., which contains six species, is described. The type species is Afipia Felis sp. nov. (the cat scratch disease bacillus). Afipia clevelandensis sp. nov., Afipia broomeae sp. nov., and three unnamed not associated with cat-borne disease. All but one strain (Afipia genospecies 3) were isolated from human wound and respiratory sources. All Afipia species are gram-negative, oxidase-positive, nonfermentative rods in the alpha-2 subgroup of the class Proteobacteria. They are motile by means of a single flagellum. They grow on buffered charcoal-yeast extract agar and nutrient broth, but rarely on MacConkey agar, at 25 and 30 degrees C. They are urease positive; but they are negative in reactions for hemolysis, indole production, H2S production (triple sugar iron agar), gelatin hydrolysis, esculin hydrolysis, and peptonization of litmus milk. They do not produce acid oxidatively from D-glucose, lactose, maltose, or sucrose. The major cell wall fatty acids are 11-methyloctadec-12-enoic (CBr19:1), cis-octadec-11-enoic (C18:1omega7c), and generally, 9,10-methylenehexadecanote and 11,12-methyleneoctadecanoate; and there are only trace amounts of hydroxy acids. The guanineplus-cytosine content is 61.5 to 69 mol%. A. Felis is positive for nitrate reduction and is delayed positive for acid production from D-xylose, but it is catalase negative. A. clevelandensis is negative in all of these tests. A. broomeae is weakly positive for catalase production and acid production from D-xylose, but it is negative for nitrate reduction.
F. D. Quinn - One of the best experts on this subject based on the ideXlab platform.
-
proposal of Afipia gen nov with Afipia Felis sp nov formerly the cat scratch disease bacillus Afipia clevelandensis sp nov formerly the cleveland clinic foundation strain Afipia broomeae sp nov and three unnamed genospecies
Journal of Clinical Microbiology, 1991Co-Authors: D. J. Brenner, C. K. English, G S Hall, D G Hollis, William F. Bibb, J Vincent, C W Moss, Kristin A Birkness, J Radosevic, F. D. QuinnAbstract:On the basis of phenotypic characterization and DNA relatedness determinations, the genus Afipia gen. nov., which contains six species, is described. The type species is Afipia Felis sp. nov. (the cat scratch disease bacillus). Afipia clevelandensis sp. nov., Afipia broomeae sp. nov., and three unnamed not associated with cat-borne disease. All but one strain (Afipia genospecies 3) were isolated from human wound and respiratory sources. All Afipia species are gram-negative, oxidase-positive, nonfermentative rods in the alpha-2 subgroup of the class Proteobacteria. They are motile by means of a single flagellum. They grow on buffered charcoal-yeast extract agar and nutrient broth, but rarely on MacConkey agar, at 25 and 30 degrees C. They are urease positive; but they are negative in reactions for hemolysis, indole production, H2S production (triple sugar iron agar), gelatin hydrolysis, esculin hydrolysis, and peptonization of litmus milk. They do not produce acid oxidatively from D-glucose, lactose, maltose, or sucrose. The major cell wall fatty acids are 11-methyloctadec-12-enoic (CBr19:1), cis-octadec-11-enoic (C18:1omega7c), and generally, 9,10-methylenehexadecanote and 11,12-methyleneoctadecanoate; and there are only trace amounts of hydroxy acids. The guanineplus-cytosine content is 61.5 to 69 mol%. A. Felis is positive for nitrate reduction and is delayed positive for acid production from D-xylose, but it is catalase negative. A. clevelandensis is negative in all of these tests. A. broomeae is weakly positive for catalase production and acid production from D-xylose, but it is negative for nitrate reduction. Images
-
Proposal of Afipia gen. nov., with Afipia Felis sp. nov. (Formerly the cat scratch disease bacillus), Afipia clevelandensis sp. nov. (Formerly the Cleveland Clinic Foundation Strain), Afipia broomeae sp. nov., and three unnamed genospecies
Journal of Clinical Microbiology, 1991Co-Authors: D. J. Brenner, C. K. English, K. A. Birkness, G S Hall, D G Hollis, William F. Bibb, J Vincent, C W Moss, J. Radošević, F. D. QuinnAbstract:On the basis of phenotypic characterization and DNA relatedness determinations, the genus Afipia gen. nov., which contains six species, is described. The type species is Afipia Felis sp. nov. (the cat scratch disease bacillus). Afipia clevelandensis sp. nov., Afipia broomeae sp. nov., and three unnamed not associated with cat-borne disease. All but one strain (Afipia genospecies 3) were isolated from human wound and respiratory sources. All Afipia species are gram-negative, oxidase-positive, nonfermentative rods in the alpha-2 subgroup of the class Proteobacteria. They are motile by means of a single flagellum. They grow on buffered charcoal-yeast extract agar and nutrient broth, but rarely on MacConkey agar, at 25 and 30 degrees C. They are urease positive; but they are negative in reactions for hemolysis, indole production, H2S production (triple sugar iron agar), gelatin hydrolysis, esculin hydrolysis, and peptonization of litmus milk. They do not produce acid oxidatively from D-glucose, lactose, maltose, or sucrose. The major cell wall fatty acids are 11-methyloctadec-12-enoic (CBr19:1), cis-octadec-11-enoic (C18:1omega7c), and generally, 9,10-methylenehexadecanote and 11,12-methyleneoctadecanoate; and there are only trace amounts of hydroxy acids. The guanineplus-cytosine content is 61.5 to 69 mol%. A. Felis is positive for nitrate reduction and is delayed positive for acid production from D-xylose, but it is catalase negative. A. clevelandensis is negative in all of these tests. A. broomeae is weakly positive for catalase production and acid production from D-xylose, but it is negative for nitrate reduction.
